IndraLab

Statements


ASXL1 affects BAP1
11 | 1 119 87
ASXL1 binds BAP1.
11 | 97 81
11 | 86 69

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

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"ASXL1 forms a complex with BRCA1 associated protein 1 (BAP1) that physically interacts with PRC2 and deubiquitinylates histone H2A [XREF_BIBR]."
| PMC

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"We investigated which BAP1 protein binding partners, and thus which regulatory complexes, mediate TRAIL sensitivity identifying the BAP1 and ASXL1 complex, the Polycomb repressive deubiquitinase (PR-DUB), as key."

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"BAP1 function is intimately linked with that of ASXL1, commonly mutated in CMML, and it was recently shown that the interaction between ASXL1 and BAP1 was restored in ASXL1-mutation corrected clones (Valletta et al, 2015)."

reach
"These data show that the specificity of the BAP1 and ASXL1 complex for the Polycomb site (K119) is not determined by the amino acids surrounding the scissile isopeptide bond, but at regions outside the ubiquitinated tail such as the nucleosome core."

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"The interaction between ASXL1 and BAP1 and the ubiquitin C-terminal hydrolase (UCH) domain of BAP1 are abso- lutely required for cooperation."

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"ASXL1 binds a deubiquitinase BAP1 to form a critical complex for H2A K119 deubiquitination through the catalysis of polycomb repressive complex 1 [XREF_BIBR, XREF_BIBR]."

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"BAP1 and ASXL1 recruitment and activation for H2A deubiquitination."

sparser
"The tumor suppressors BAP1 and ASXL1 interact to form a polycomb deubiquitinase complex that removes monoubiquitin from histone H2A lysine 119 (H2AK119Ub)."
| PMC

sparser
"Additional sex combs-like 1 (ASXL1) is frequently mutated in a variety of myeloid malignancies, resulting in expression of a C-terminal-truncated ASXL1 protein that confers gain of function on the ASXL1-BAP1 deubiquitinase (DUB) complex."
Mutated ASXL1 binds BAP1. 9 / 9
| 9

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"This hyperactive mutant ASXL1/BAP1 complex is thought to promote impairment of myeloid differentiation through inhibition of H2AK119ub at posterior HOXA genes and IRF8, encoding an essential transcription factor in myeloid lineages [18]."

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"This hyperactive mutant ASXL1 and BAP1 complex induces upregulation of posterior HOXA genes and IRF8 through inhibition of H2AK119ub, impairing multilineage differentiation of haematopoietic progenitors (except for that toward monocytes), and accelerates RUNX1-ETO-induced leukaemogenesis."

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"Mechanistically, the mutant ASXL1 and BAP1 complex induced the upregulation of HOXA5, HOXA7, HOXA9, and IRF8 via a reduction in H2AK119ub."

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"Cancer associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1 and BAP1 complex."

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"[Molecular mechanisms by which the mutant ASXL1 and BAP1 complex aggravates myeloid leukemia]."

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"We have examined the role of H2AK119Ub in regulating gene expression by using hyperactive mutations of the ASXL1 and BAP1 complex that allowed us to deplete ~ 90% of total H2AK119Ub."

reach
"Based of the possibility that ASXL1 truncations might act as gain-of-function mutations of the ASXL1 and BAP1 complex, we examined whether hyperactive ASXL-BAP1 complexes could alter the fate of haematopoietic cells in vivo."

reach
"The mutant ASXL1/BAP1 complex inhibits the differentiation of HSPCs toward multilineage except for immature monocytes, and promoted myeloid leukemogenesis induced by RUNX1-ETO, an oncogenic fusion protein frequently coexisting with ASXL1 mutations [72]."

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"Our studies here raise the possibility that ASXL1 mutations may act as gain-of-function mutations of the ASXL1 and BAP1 complex."
ASXL1 binds EED and BAP1. 4 / 4
| 4

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

sparser
"BAP1 interacts with ASXL1 polycomb chromatin binding protein forming a polycomb repressive deubiquitinase complex that regulates histone H2A ubiquitination xref ."

sparser
"BAP1 also interacts with ASXL1 to form the Polycomb group repressive deubiquitinase complex (PR-DUB), which is involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"8 BAP1 interacts with ASXL1 (MIM 612990 ) to form the Polycomb group Repressive Deubiquitinase complex (PR-DUB)."
ASXL1 binds ASXL2 and BAP1. 3 / 3
| 3

sparser
"Although both ASXL1 and ASXL2 have been identified to bind to BAP1, recent biochemical characterization of BAP1 complexes by xref suggest that BAP1 forms two mutually exclusive complexes with ASXL1 and ASXL2."

sparser
"Depletion of the PHD fingers did not affect the stability of ASXL1 or ASXL2, or the interaction between ASXL1, ASXL2, and BAP1 (Additional file xref : Fig. S2A, B)."

sparser
"Proteomic analysis of BAP1 interacting partners using this system revealed that BAP1 interacts with ASXL1 and ASXL2 in vivo as well as host cell factor 1 (HCF-1), O-GlcNAc transferase (OGT), and Forkhead box protein K1/2 (FOXK1/2) ( xref )."
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
ASXL1 binds BAP1 and ASXH domain. 2 / 2
| 2

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"As mentioned earlier, the interaction between BAP1 and ASXL1 occurs through the ASXH domain."

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"ASXL1 directly binds to BAP1 through the ASXH domain and its adjoining region."
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
AKT binds ASXL1 and BAP1. 1 / 1
| 1

sparser
"We found that wild-type/mutant ASXL1 and BAP1 bound to AKT in both cytoplasm and nucleus in 293T cells, suggesting that they form a complex (Fig.  xref a, xref , and Supplementary Fig.  xref )."
ASXL1 activates BAP1.
| 1 22 6
ASXL1 activates BAP1. 10 / 29
| 1 22 6

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"In this paper we describe that similar to the homologous UCH-L5 and RPN13 DEU complex, the DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing BAP1 's affinity specifically for the ubiquitin in the substrate, through a combination of mild effects."

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"Like UCH37, BAP1 associates with a partner protein, ASXL1, which activates BAP1 and is required for the BAP1 mediated deubiquitylation of H2A in nucleosomes."

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"As part of the Polycomb repression machinery, BAP1 is activated by the deubiquitinase adaptor domain of ASXL1 mediating gene repression by cleaving ubiquitin (Ub) from histone H2A in nucleosomes."

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"BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression, but the mechanism of this reaction remains poorly defined."

sparser
"A recent study suggests that ASXL1 might activate Bap1 in a similar manner xref ."

sparser
"BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression, but the mechanism of this reaction remains poorly defined."

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"ASXL1 DEU stimulates BAP1 by lowering the K M on Ub-AMC (XREF_SUPPLEMENTARY)."

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"A previous report showed that the deubiquitinase adapter (DEUBAD) domain of ASXL1 increases BAP1 's affinity for ubiquitin on H2A 15, and we showed that monoubiquitinated ASXL1-MT enhances nuclear retention of BAP1."

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"BAP1 is activated by ASXL1 in a similar manner where the DEUBAD domain of ASXL1 increases affinity of BAP1 for the ubiquitinated substrate [98]."

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"In summary, we have shown that ASXL1 DEU activates BAP1 by increasing the affinity for ubiquitin through a combination of mild effects including stabilization of the BAP1 ULD anchor."
BAP1 affects ASXL1
11 | 101 81
BAP1 binds ASXL1.
11 | 97 81
11 | 86 69

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

reach
"ASXL1 forms a complex with BRCA1 associated protein 1 (BAP1) that physically interacts with PRC2 and deubiquitinylates histone H2A [XREF_BIBR]."
| PMC

reach
"We investigated which BAP1 protein binding partners, and thus which regulatory complexes, mediate TRAIL sensitivity identifying the BAP1 and ASXL1 complex, the Polycomb repressive deubiquitinase (PR-DUB), as key."

reach
"BAP1 function is intimately linked with that of ASXL1, commonly mutated in CMML, and it was recently shown that the interaction between ASXL1 and BAP1 was restored in ASXL1-mutation corrected clones (Valletta et al, 2015)."

reach
"These data show that the specificity of the BAP1 and ASXL1 complex for the Polycomb site (K119) is not determined by the amino acids surrounding the scissile isopeptide bond, but at regions outside the ubiquitinated tail such as the nucleosome core."

reach
"The interaction between ASXL1 and BAP1 and the ubiquitin C-terminal hydrolase (UCH) domain of BAP1 are abso- lutely required for cooperation."

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"ASXL1 binds a deubiquitinase BAP1 to form a critical complex for H2A K119 deubiquitination through the catalysis of polycomb repressive complex 1 [XREF_BIBR, XREF_BIBR]."

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"BAP1 and ASXL1 recruitment and activation for H2A deubiquitination."

sparser
"The tumor suppressors BAP1 and ASXL1 interact to form a polycomb deubiquitinase complex that removes monoubiquitin from histone H2A lysine 119 (H2AK119Ub)."
| PMC

sparser
"Additional sex combs-like 1 (ASXL1) is frequently mutated in a variety of myeloid malignancies, resulting in expression of a C-terminal-truncated ASXL1 protein that confers gain of function on the ASXL1-BAP1 deubiquitinase (DUB) complex."
Mutated ASXL1 binds BAP1. 9 / 9
| 9

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"This hyperactive mutant ASXL1/BAP1 complex is thought to promote impairment of myeloid differentiation through inhibition of H2AK119ub at posterior HOXA genes and IRF8, encoding an essential transcription factor in myeloid lineages [18]."

reach
"This hyperactive mutant ASXL1 and BAP1 complex induces upregulation of posterior HOXA genes and IRF8 through inhibition of H2AK119ub, impairing multilineage differentiation of haematopoietic progenitors (except for that toward monocytes), and accelerates RUNX1-ETO-induced leukaemogenesis."

reach
"Mechanistically, the mutant ASXL1 and BAP1 complex induced the upregulation of HOXA5, HOXA7, HOXA9, and IRF8 via a reduction in H2AK119ub."

reach
"Cancer associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1 and BAP1 complex."

reach
"[Molecular mechanisms by which the mutant ASXL1 and BAP1 complex aggravates myeloid leukemia]."

reach
"We have examined the role of H2AK119Ub in regulating gene expression by using hyperactive mutations of the ASXL1 and BAP1 complex that allowed us to deplete ~ 90% of total H2AK119Ub."

reach
"Based of the possibility that ASXL1 truncations might act as gain-of-function mutations of the ASXL1 and BAP1 complex, we examined whether hyperactive ASXL-BAP1 complexes could alter the fate of haematopoietic cells in vivo."

reach
"The mutant ASXL1/BAP1 complex inhibits the differentiation of HSPCs toward multilineage except for immature monocytes, and promoted myeloid leukemogenesis induced by RUNX1-ETO, an oncogenic fusion protein frequently coexisting with ASXL1 mutations [72]."

reach
"Our studies here raise the possibility that ASXL1 mutations may act as gain-of-function mutations of the ASXL1 and BAP1 complex."
ASXL1 binds EED and BAP1. 4 / 4
| 4

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

sparser
"BAP1 interacts with ASXL1 polycomb chromatin binding protein forming a polycomb repressive deubiquitinase complex that regulates histone H2A ubiquitination xref ."

sparser
"BAP1 also interacts with ASXL1 to form the Polycomb group repressive deubiquitinase complex (PR-DUB), which is involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"8 BAP1 interacts with ASXL1 (MIM 612990 ) to form the Polycomb group Repressive Deubiquitinase complex (PR-DUB)."
ASXL1 binds ASXL2 and BAP1. 3 / 3
| 3

sparser
"Although both ASXL1 and ASXL2 have been identified to bind to BAP1, recent biochemical characterization of BAP1 complexes by xref suggest that BAP1 forms two mutually exclusive complexes with ASXL1 and ASXL2."

sparser
"Depletion of the PHD fingers did not affect the stability of ASXL1 or ASXL2, or the interaction between ASXL1, ASXL2, and BAP1 (Additional file xref : Fig. S2A, B)."

sparser
"Proteomic analysis of BAP1 interacting partners using this system revealed that BAP1 interacts with ASXL1 and ASXL2 in vivo as well as host cell factor 1 (HCF-1), O-GlcNAc transferase (OGT), and Forkhead box protein K1/2 (FOXK1/2) ( xref )."
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
ASXL1 binds BAP1 and ASXH domain. 2 / 2
| 2

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"As mentioned earlier, the interaction between BAP1 and ASXL1 occurs through the ASXH domain."

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"ASXL1 directly binds to BAP1 through the ASXH domain and its adjoining region."
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
AKT binds ASXL1 and BAP1. 1 / 1
| 1

sparser
"We found that wild-type/mutant ASXL1 and BAP1 bound to AKT in both cytoplasm and nucleus in 293T cells, suggesting that they form a complex (Fig.  xref a, xref , and Supplementary Fig.  xref )."
BAP1 ubiquitinates ASXL1.
| 2
BAP1 leads to the ubiquitination of mutated ASXL1. 2 / 2
| 2

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"BAP1 induces stabilization and monoubiquitination of mutant ASXL1, and monoubiquitinated ASXL1-MT increases the catalytic function of BAP1."

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"BAP1 induces monoubiquitination of mutant ASXL1."
BAP1 activates ASXL1.
| 2
BAP1 activates ASXL1. 2 / 2
| 2

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"Truncated ASXL1 increases BAP1 protein stability, enhances BAP1 recruitment to chromatin and promotes the expression of a pro-leukemic transcriptional signature."

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"Reduced BAP1 activity prevents ASXL1 truncation driven myeloid malignancy in vivo."
BAP1 affects BRCA1
4 1 1 | 56 45
BAP1 binds BRCA1.
4 1 | 24 45
4 1 | 23 38

sparser
"31,32 Because BAP1 can interact with BRCA1 and because BAP1FL reconstitution in BAP1-null cell lines leads to resistance to PARP inhibition (unlike in BAP1Δ-reconstituted ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"These data reveal that the interaction between BAP1 and BRCA1 is specific (BAP1 does not bind to any other RING finger domain tested to date) and suggests that the absence of BAP1 binding to mutat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"BAP1-BRCA1 interaction is however still controversial [ xref ]."

sparser
"Although the direct interaction of BAP1 with BRCA1 remains controversial [ xref ], several studies report a potential role of BAP1 in DNA damage repair, and particularly in homologous recombination (HR)."

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"Although the molecular genetics of cutaneous melanomas have been investigated in numerous studies [XREF_BIBR, XREF_BIBR, XREF_BIBR], the genetic events that lead to the development of CBN, MABN or ACBN are poorly understood and are limited to a few genes [XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR], including BAP1 (BRCA1 binding protein 1), an oncogenic deubiquitinase, which has been found to be mutated in metastatic uveal melanomas and MABNs [XREF_BIBR]."

sparser
"This suggests that the effects of BAP1 on foci assembly could involve direct histone H2A-deubiquitination, although other mechanisms, for example involving the interaction of BAP1 with BRCA1 ( Jensen [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Several lines of evidences indicate that PARP inhibitor administration might be an effective treatment in presence of BAP1 and/or RAD21 alterations since both BAP1 and RAD21 are involved in the DNA repair pathway, BAP1 interacts with BRCA1 and BRCA1-mediated DNA repair pathway alterations enhance the sensitivity to PARP inhibitor administration."

sparser
"Because of the involvement of RAD21 in the DNA repair pathway, the interaction of BAP1 with BRCA1 and the enhanced sensitivity to PARP inhibitor administration in presence of alterations in the BRCA1-mediated DNA repair pathway, it was decided first to treat the patient with FOLFIRI every 2 weeks [irinotecan 180 mg/m 2 , folinic acid 400 mg/m 2 , 5-fluorouracil (5-FU) 400 mg/m 2 intravenous infusion bolus, then 5-FU 2400 mg/m 2 intravenous infusion over 46 h] and then to start a PARP inhibitor."

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"However, it is possible that BAP1 interacts with BRCA1 under a particular set of circumstances, such as in response to DNA damage."

sparser
"Thus, NUMB enhances Notch signaling by regulating the ubiquitinating activity of the BAP1BRCA1 complex."
BRCA1 binds BAP1 and BARD1. 6 / 6
| 1 5

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear and needs to be studied in more detail."

sparser
"BAP1 interacts with BARD1 and BRCA1 and inhibits the E3 ligase activity of BRCA1/BARD1."

sparser
"The ring domains of BRCA1 (residues 1-109) are associated with BARD1 [ xref ] and BAP1 [ xref ]."

sparser
"BAP1 and BRCA1-BARD1 complex coordinately regulate ubiquitination during the DNA damage response and the cell cycle [ xref , xref ]."

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"Until recently, the combination of pemetrexed and cisplatin was the only approved first-line therapy, yielding a median overall survival (OS) of 12.1 months.2 Nivolumab plus ipilimumab was approved recently as initial treatment for malignant pleural mesothelioma (MPM) on the basis of a phase 3 clinical trial that resulted in a median OS of 18.1 months.3 4 There are no approved therapies for patients with tumor relapse after first-line therapies.Risk factors in developing mesothelioma include exposure to asbestos5 and other carcinogenic fibers such as erionite,6 and previous radiation therapy.7 8 Studies of familial clusters of mesothelioma have revealed genetic predisposition to develop MM.9 10 Recent work has identified germline mutations in BAP1 that can predispose to mesothelioma11 12 and other cancers such as uveal and cutaneous melanoma, basal cell carcinoma, meningioma, and renal cell cancers.13 14 We previously identified deleterious germline mutations in 12% of 241 consecutive patients with MM who enrolled on a prospective natural history study, with BAP1 being the most frequent germline mutation.15 BAP1 was also recurrently inactivated at the somatic level, suggesting BAP1 variants undergoing positive selection in the context of the classic “two-hit” model.BAP1, a nuclear deubiquitylase, was initially discovered as an interaction partner of the tumor suppressor BRCA1.16 Subsequent studies revealed that BAP1 binds to BARD1, which, in turn, binds to BRCA1, forming the BRCA1-BARD1-BAP1 complex that is crucial for the homologous recombination (HR)–mediated repair of DNA double-strand breaks (DSBs).17 PARP enzymes play a major role in DNA single-strand break repair and base excision repair pathways."

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear."
BRCA1 binds BAP1 and RING. 2 / 2
| 2

sparser
"BAP1 interacts with the RING domain of human BRCA1, a predominately nuclear, ovarian and breast cancer susceptibility gene product with tumor suppressor activity."

sparser
"The BRCA1 RING domain also binds BAP1 (BRCA1-associated protein-1), a de-ubiquitinating enzyme 26 ."
BAP1 inhibits BRCA1.
| 15
BAP1 inhibits BRCA1. 10 / 18
| 14

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"BAP1 enhances BRCA1 mediated suppression of cell growth in colony formation assays, and this suppression by BAP1 is augmented by its UCH enzymatic domain."

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"BAP1 enhances BRCA1 mediated inhibition of breast cancer cell growth and is the first nuclear localized ubiquitin carboxy-terminal hydrolase to be identified."

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"In our study, we found that BAP1 depletion decreased the assembly of constitutive BRCA1 foci, which are associated with HR, but had minimal effect on gamma-H2AX foci."

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"Biochemical studies revealed that BARD1 potentiates the ubiquitin ligase activity of BRCA1, which can be reversed through the inhibition of BRCA1-BARD1 association by BRCA1 associated protein 1 (BAP1) 64."

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"A previous study on the expression of BAP1 tumor suppressor gene in 1222 patients with TCGA breast cancer data reported that the expression of BAP1, which enhances BRCA1-mediated suppression of cell proliferation through BRCA1 stabilization, is highly correlated with USP19 expression."
| PMC

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"XREF_BIBR suggested that BAP1 inhibits the E3 ligase activity of BRCA1 in a manner independent of its deubiquitination activity."

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"XREF_BIBR It was initially suggested that BAP1 bound BRCA1 cleaves ubiquitin, and enhances the growth-suppressive effects of BRCA1."

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"We further found that BAP1 depletion suppressed the assembly of constitutive BRCA1 foci, which are associated with homologous recombination (HR), but had minimal effect on gamma-H2AX foci and did not affect proteins associated with nonhomologous end joining, suggesting that BAP1 affects radiosensitivity in HNSCC by modifying HR."

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"Early experiments found that BAP1 enhanced BRCA1 mediated inhibition of breast cancer cell growth."

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"BAP1 enhances BRCA1 mediated suppression of cell proliferation through stabilizing BRCA1 [XREF_BIBR]."
BAP1 bound to BARD1 inhibits BRCA1. 1 / 1
| 1

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"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1 and BARD1."
BAP1 activates BRCA1.
| 10
BAP1 activates BRCA1. 10 / 13
| 10

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"Surprisingly, BAP1 mediated growth suppression is independent of wild-type BRCA1."

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"XREF_BIBR suggested that BAP1 could function for HR repair in the BRCA1 related pathway, and they hypothesized that BAP1 enables BRCA1 to readily accumulate DSB sites."

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"Initially identified in a yeast two-hybrid screen using the RING-finger domain of the breast cancer type 1 susceptibility protein BRCA1 as bait, BAP1 was shown to enhance BRCA1 mediated growth suppression in MCF7 cells [XREF_BIBR]."

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"BAP1 can deubiquitylate Ub-H2AK119 at the site of UV-induced DNA damage, and promote DNA repair by regulating the accumulation of repair proteins (BRCA1, RAD51, and RPA) (7)."

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"Interestingly, BRCA1 proteins were decreased in the MM cells with BAP1 deletion, and transduction of the mutants as well as WT BAP1 increased BRCA1 proteins, suggesting that BAP1 may promote DNA repair partly through stabilizing BRCA1."

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"The association of BAP1, a Ub carboxy-terminal hydrolase, suggests that BRCA1 mediated ubiquitination is tightly regulated, and there is evidence that BAP1 can potentiate BRCA1 tumor suppressor functi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"It is still not known whether BAP1 functions to modulate BRCA1 ubiquitynating activity and/or to regulate ubiquitin dependent degradation of BRCA1 itself."

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"BRCA1-associated protein 1 (BAP1) is the translation product of BRCA1 and belongs to the family of deubiquitinating enzymes."

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"Conceivably, BAP1 might modulate BRCA1 mediated tumor suppression by hydrolyzing ubiquitin moieties from the BRCA1 and BARD1 heterodimer, its associated proteins, or even its substrates [61]."

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"Although BAP1 has been hypothesized to function in BRCA1 mediated processes, our results, and others ', support the possibility of BRCA1 independent functions of BAP1."
BAP1 increases the amount of BRCA1.
| 5
BAP1 increases the amount of BRCA1. 5 / 5
| 5

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"XREF_BIBR previously suggested that BAP1 enhanced BRCA1 mediated inhibition of cancer cell growth, although they did not directly establish the possibility that BAP1 enhances the BRCA1 protein level."

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"BAP1 mediated growth suppression is independent of wild-type BRCA1 expression."

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"Noticeably, we found that exogenous BAP1 significantly increased the BRCA1 protein level."

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"Although BRCA1 expression significantly decreased in an MM cell line with BAP1 deletion, Y-MESO-25, the transduction of the WT and even mutant forms of BAP1 induced upregulation of the BRCA1 protein level."

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"It is worth noting that BAP1 can restore the protein expression of BRCA1."
BAP1 deubiquitinates BRCA1.
1 | 2
BAP1 deubiquitinates BRCA1. 3 / 3
1 | 2

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"As a possible mechanism of BRCA1 stabilization by BAP1 transduction in this study, we speculated that the ubiquitination of BRCA1 protein might be directly deubiquitinated by BAP1."

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"We hypothesized that BAP1 might deubiquitinate BRCA1 and protect it from proteasome mediated degradation; this might explain the BRCA1 stabilization by the mutant BAP1 forms that retained the DUB activity."

"BAP1 also controls G1/S cell cycle progression by regulating BRCA-1 [111], Ying Yang 1 (YY-1), and host cell factor 1 (HCF-1)"
BAP1 affects HCFC1
18 1 | 55 37
BAP1 binds HCFC1.
18 | 42 37
18 | 38 32

No evidence text available

sparser
"BAP1 is a 729 amino acid nuclear ubiquitin hydrolase with multiple functional domains and it has, therefore, been implicated in several cellular processes such as cell proliferation, DNA repair response, and chromatin dynamics. xref Specifically, an interaction between BAP1 and host cell factor 1 (HCF1), which interacts with histone-modifying complexes during cell division, has been described. xref More recently, it has been shown that BAP1, through an interaction with ASXL1 forms the polycomb group repressive deubiquitinase complex, which affects stem cell pluripotency. xref "

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"2 Specifically, an interaction between BAP1 and host cell factor 1 (HCF1), which interacts with histone modifying complexes during cell division, has been described."

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"In fact, BAP1 binds and deubiquitinates the transcriptional regulator host cell factor 1 that interacts with histone modifying complexes [19]."

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"Thus, BAP1 binds HCF-1, and binding to HCF-1, but not H2Aub1 deubiquitination, is important for the inhibition of cell proliferation."

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"Knock-in mice expressing BAP1 with a 3xFlag tag revealed that BAP1 interacts with HCF-1, OGT, and the polycomb group proteins ASXL1 and ASXL2 in vivo."

sparser
"BAP1 binds to HCF-1 in renal cancer cell lines. xref BAP1 binding was demonstrated through reciprocal immunoprecipitation experiments, and most BAP1 protein co-fractionated with HCF-1 by gel filtration chromatography. xref BAP1 binding to and co-fractionation with HCF-1 was also observed in orthotopic tumorgrafts in mice directly derived from surgically removed tumors of patients. xref Consistent with previous observations, xref immunoprecipitations of BAP1 and HCF-1 deplete BAP1 protein to a similar extent suggesting that most BAP1 is bound to HCF-1 (at least in cells reconstituted with BAP1). xref Binding to HCF-1 is important for BAP1-supression of cell proliferation. xref BAP1 reintroduction into two different BAP1-deficient ccRCC cell lines reduced cell growth. xref The inhibition of cell proliferation by BAP1 was compromised by disruption of the HCF-1 binding motif. xref Taken together these data suggest that BAP1 binding to HCF-1 is important for its tumor suppressor function in renal cancer."

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"The HCFC1 and BAP1 complex contributes to cell cycle progression and BAP1 deubiquitinating activity is probably involved in HCFC1 turnover."

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"Finally, monoubiquitination of the DEUBAD is not impaired when this domain is expressed with BAP1 suggesting that BAP1 interaction with HCF-1 is not required for this modification (Fig. 2d)."

No evidence text available
YY1 binds HCFC1 and BAP1. 7 / 7
| 2 5

sparser
"BAP1 forms a ternary complex with HCF1 and YY1, which acts as transcriptional repressor of genes involved in cell proliferation ( xref )."

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"31 Further, BAP1 forms a protein complex with the host cell factor 1 and Yin and Yang 1."

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"3,10,12 BAP1 and HCF-1 can also form a complex with the transcription factor YY1, which recruits BAP1 and HCF-1 to various promoters to regulate gene expression."

reach
"Prominent among the positively enriched gene sets were those related to writers of histone H3K27Me3, including PRC2 and SUZ12 (XREF_FIG), as well as genes participating in cell proliferation and transcription regulation involving ubiquitin signaling mediated by the Bap1, HCF1, and YY1 complex, EED related stem cell pluripotency, cytokine signaling, and cell adhesion; 100 of the top pathways differentially affected in MMs from TKO versus DKO mice are presented in XREF_SUPPLEMENTARY."

reach
"Our RNA-seq analysis revealed that MMs from Bap1; Nf2; Cdkn2a CKO mice exhibit positively enriched gene sets consistent with cellular processes previously implicated in Bap1 function, such as genes mediated by the Bap1, HCF1, and YY1 complex, which play a role in cellular proliferation and transcription regulation involving ubiquitin signaling."

sparser
"Yu H, Mashtalir N, Daou S, et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression."

sparser
"Finally, BAP1 forms complexes with the transcription factors Yin Yang 1 (YY1) and host cell factor 1 (HCF-1), and controls cell-cycle progression at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters [ xref , xref ]."
HCFC1 binds OGT and BAP1. 2 / 2
| 2

reach
"The fact that HCF-1 acts as an important modulator of cellular circadian rhythm [XREF_BIBR] raises the possibility that the OGT, HCF-1, and BAP1 complex interacts with the BMAL1, CLOCK, and PER complex to modulate circadian rhythm."

reach
"Within the BAP1/HCF-1/OGT complex, HCF1 is modified by O-GlcNacylation, the addition of single O-linked N-acetylglucosamine moiety to specific serine or threonine residues (50,51)."
BAP1 deubiquitinates HCFC1.
1 | 6
BAP1 deubiquitinates HCFC1. 7 / 7
1 | 6

reach
"HCF-1 is modified with ubiquitin in vivo, and ectopic studies suggest BAP1 deubiquitinates HCF-1."

reach
"It has been proposed that BAP1 may modulate cell proliferation by deubiquitylating the transcriptional regulator HCF1."

reach
"BAP1 also interacts with and deubiquitinates the transcriptional regulator host cell factor 1 (HCF1)."

reach
"The massive increase of BAP1 expression with tumor cell proliferation was expected, as BAP1 regulates cell proliferation via deubiquitination of its target protein host cell factor-1 (HCF1), which plays a critical role at multiple stages of the cell cycle [55, 56]."

"The tumor suppressor BAP1 associates with ASXL1/2 to form the core Polycomb complex PR-DUB, which catalyzes the removal of mono-ubiquitin from several substrates including histone H2A. This complex also mediates the poly-deubiquitination of HCFC1, OGT and PCG1-α, preventing them from proteasomal degradation."

reach
"It has been proposed that ubiquitylation of HCF1 blocks E2F responsive promoter activity, and that HCF1 deubiquitylation by BAP1 would remove this inhibition and promote cell proliferation 33."

reach
"SETD2 is a histone H3 lysine 36 (H3K36) methyltransferase that regulates mRNA splicing and transcription elongation; BAP1 interacts with and deubiquitinates host cell factor-1 (HCF-1), a transcription co-activator, that regulates cell proliferation; and KDM5C a histone 3 trimethyl-lysine 4 (H3K4Me3) demethylase that erase active transcription marks."
BAP1 activates HCFC1.
| 4
BAP1 activates HCFC1. 4 / 5
| 4

reach
"The BAP1 nuclear deubiquitinase is known to target histones (together with ASXL1 as a Polycomb repressor subunit) and the HCF1 transcriptional co-factor, and we show that BAP1 knockdown in MPM cell lines affects E2F and Polycomb target genes."

reach
"BAP1 is a deubiquitinase targeting histones and the host cell factor-1 transcriptional co-factor, and plays a role in transcriptional regulation, chromatin modulation, cell cycle regulation, and DNA repair."

reach
"In a mouse model, absence of one such shared binding partner, BAP1, led to a lack of HCFC1 protein (since BAP1 normally prevents HCFC1 degradation), and these mice exhibited features of myeloproliferative disease."

reach
"Among these substrates, OGT and HCF-1 levelswere found to decrease upon Bap1 deletion in mice (Dey et al., 2012), supporting a previous report on the stabilization of HCF-1 by BAP1 (Machida et al., 20[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
BAP1 inhibits HCFC1.
| 1
BAP1 inhibits HCFC1. 1 / 2
| 1

reach
"BAP1 loss induces a modest over-accumulation of HCF-1, leading G1 to S phase cell cycle transition, promoting cell proliferation (4)."
BAP1 increases the amount of HCFC1.
| 2
BAP1 increases the amount of HCFC1. 2 / 2
| 2

reach
"OGT and HCF-1 levels were decreased by Bap1 deletion, indicating a critical role for BAP1 in stabilizing these epigenetic regulators."

reach
"OGT and HCF-1 levels were decreased by Bap1 deletion, and were associated with myeloid transformation ."
BAP1 is modified
17 | 62 15
BAP1 is phosphorylated.
17 | 27 15
BAP1 is phosphorylated. 10 / 33
| 21 12

sparser
"As a fraction of the F679Lfs37X BAP1 mutant protein resides in the nucleus (Fig. xref , fourth lane from the right), even if much less abundant than in the cytoplasm, the remarkably increased p-S592 BAP1 signal of F679Lfs37X may just reflect the phosphorylation of the mutant BAP1 that has been localized in the nucleus from the beginning."

sparser
"Interestingly, BAP1 phosphorylation is cell cycle regulated, with a peak of modification at the G1/S transition."

sparser
"Therefore, it is possible that upon DNA damage, BAP1 is phosphorylated and its function modified to mediate growth suppression."

rlimsp
"Additionally, it was intriguing to find that phosphorylation of mutant BAP1 seemed to induce its translocation from the cytoplasm to the nucleus, as Western blot analysis detected strong p-S592 BAP1 in the nucleus of the mutant F679LfsX37-transduced cells (Fig.4d, second lane from the right)."

sparser
"Recruitment to DNA double-strand break sites is mediated via phosphorylation of BAP1, and the role of BAP in DNA damage response involves its catalytic activity [ xref ]."

rlimsp
"In this matter, the regulation of its ubiquitination and the activity of the kinases responsible for BAP1 phosphorylation are not well defined and could be altered in the tumoral context."

sparser
"Such dissociation of DUBs induced by ATM-dependent phosphorylation has been reported for another UCH family DUB, BAP1, the substrate of which is ubiquitylated H2A. Phosphorylation of BAP1 that promotes HR resulted in reduced chromatin binding of BAP1 xref ."

rlimsp
"Irradiation of MM cells induces phosphorylation of BAP1."

rlimsp
"(e) Exogenous BAP1 increased both BRCA1 protein and its phosphorylation levels."

rlimsp
"Finally, we identified six IR-induced phosphorylation sites in BAP1 and showed that mutation of these residues inhibits BAP1 recruitment to DSB sites."
BAP1 is phosphorylated on S592. 10 / 10
2 | 5 3

rlimsp
"In this study we characterized DNA damage-induced phosphorylation of BAP1 at serine 592 (pS592) and the cellular outcomes of this modification."

sparser
"In particular, the phosphatidylinositol 3-kinase-related kinases ATM/ATR/DNA-PK phosphorylate BAP1 at S592, which is one of the five serines in its carboxyl terminus that are modified in response to DNA damage [ xref , xref , xref , xref ]."

No evidence text available

sparser
"Phosphorylation of BAP1 at S592 is an important regulatory mechanism to dissociate BAP1 from chromatin and regulate-specific genes during DNA replication and repair xref ."

sparser
"BAP1 is also phosphorylated at serine 592 in response to DNA damage."

sparser
"Phosphorylation of BAP1 at serine 592 in response to DNA damage by ATM/ATR kinases retains BAP1 in the nucleus; however, it is predominantly dissociated from chromatin, suggesting that this phosphorylation removes BAP1 from specific promoters [ xref ]."

rlimsp
"Eletr et al.27 reported that BAP1 is phosphorylated at serine 592 following DNA damage, that phospho-BAP1 causes deubiquitination of H2AK119ub1, and that this signal promotes the HR pathway."

sparser
"In this study we characterized DNA damage-induced phosphorylation of BAP1 at serine 592 (pS592) and the cellular outcomes of this modification."

No evidence text available

rlimsp
"BAP1 is phosphorylated at serine 592 in S-phase following DNA damage."
BAP1 is phosphorylated on S597. 4 / 4
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
BAP1 is phosphorylated on S583. 3 / 3
2 | 1

No evidence text available

sparser
"However, several studies have shown that BAP1 Ser583 is phosphorylated in human cells after UV light exposure, providing functional significance of the serine at position 583 ( xref , xref )."

No evidence text available
BAP1 is phosphorylated on S384. 2 / 2
2 |

No evidence text available

No evidence text available
BAP1 is phosphorylated on S596. 2 / 2
2 |

No evidence text available

No evidence text available
BAP1 is phosphorylated on S395. 2 / 2
2 |

No evidence text available

No evidence text available
BAP1 is phosphorylated on S582. 2 / 2
2 |

No evidence text available

No evidence text available
BAP1 is phosphorylated on S464. 1 / 1
1 |

No evidence text available
BAP1 is ubiquitinated.
| 20
BAP1 is ubiquitinated. 10 / 20
| 20

sparser
"Then ubiquitinated BAP1 was also evaluated by anti-BAP1 IP followed by anti-Myc western analysis (Fig. xref c,d)."

sparser
"However, BAP1 can be ubiquitylated by ubiquitin-conjugating enzyme E2 O (UBE2O) at its NLS to promote nuclear export xref ."

sparser
"Controlling UBE2O import itself would provide another level of regulation for the fine-tuning of BAP1 ubiquitination."

sparser
"On the other hand, since ubiquitination of BAP1 is counteracted by autodeubiquitination, one can predict that a yet-to-be-identified signal is needed to inactivate BAP1 catalytic activity."

sparser
"Notably, we did not detect ubiquitination of wild-type BAP1 as opposed to C91S, and this ubiquitination was completely abolished by the UBE2O inhibitor PAO ( Figures 2 A and S2 B)."

sparser
"An siRNA screen for DUBs that affect levels of ubiquitylated γ-tubulin identified BAP1 and UCHL1 as candidates [ xref ]."

sparser
"Further, deletion of N or C terminal regions of UBE2O has been found to entirely disrupt ubiquitylation of BAP1 [ xref ]."

sparser
"Therefore, we evaluated whether the ubiquitination of BAP1 affects its subcellular localization."

sparser
"UBE2O could be imported into the nucleus to ubiquitinate BAP1 and promote its ubiquitin-mediated nuclear export as observed for other proteins ( Groulx and Lee, 2002; Li et al., 2003 )."

sparser
"This is also due to the ubiquitination of BAP1 near the NLS by UBE2O. Studies have shown that the auto-deubiquitination of BAP1 can counteract this process through intramolecular interactions, thereby ensuring its function in tumour suppression ( xref ; xref )."
BAP1 is methylated.
| 14
BAP1 is methylated. 10 / 14
| 14

sparser
"Therefore, we investigated whether BAP1 body methylation may influence BAP1 gene expression."

sparser
"To determine whether the low BAP1 mRNA levels in class 2 tumors without detectable BAP1 mutations may be explained by DNA methyation, we performed a preliminary analysis of DNA methylation of BAP1."

sparser
"Similarly, no BAP1 methylation alterations were found in clear cell renal carcinoma xref ."

sparser
"There are also no reports that review the methylation of BAP1 in ICC or GBC."

sparser
"BAP1 methylation: a prognostic marker of uveal melanoma metastasis."

sparser
"Here, we show that assessment of BAP1 methylation is an accurate and readily clinically actionable assay to accurately identify high-risk uveal melanoma patients."

sparser
"Interestingly, a recent study also showed that BAP1 methylation at a single genomic locus is strongly correlated with BAP1 mutations, loss of BAP1 genomic copy and BAP1 protein levels [126]."

sparser
"BAP1 promoter and body Methylation are not altered in Malignant Mesothelioma."

sparser
"By analyzing 22 sporadic MM biopsies, Nasu and colleagues found that BAP1 promoter methylation was not altered in MM ( xref )."

sparser
"Besides this, higher levels of BAP1 methylation significantly correlated with worse survival in UM patients [126]."
BAP1 is glycosylated.
| 1
BAP1 is glycosylated. 1 / 1
| 1

sparser
"Consistent with this, Jensen et al , who first described BAP1, identified a number of putative N‐linked BAP1 glycosylation sites, suggesting that BAP1 may traverse the Golgi during maturation xref ."
| 1 42 4

reach
"BAP1 enhances BRCA1 mediated suppression of cell proliferation through stabilizing BRCA1 [XREF_BIBR]."

reach
"BAP1 suppresses MM cell proliferation."

reach
"In contrast to melanoma cells, stable overexpression of BAP1 into immortalized but non transformed melanocytes did suppress proliferation and reduce survivin."

eidos
"Results BAP1 promotes proliferation in HNSCC cells regardless of HPV status Studies of the effects of BAP1 on cell proliferation have shown conflicting results , with some reporting that BAP1 suppresses cell proliferation ( 12 , 13 ) , and others reporting that BAP1 enhances proliferation ( 14 , 15 ) ."

reach
"As shown in XREF_FIG, stable introduction of BAP1 into Pmel cells (i.e. Pmel (BAP1)) reduced proliferation, colony forming capacity and survivin levels."

reach
"Wild-type BAP1 but not mutant BAP1 significantly inhibited HCC cell proliferation, invasion, epithelial-mesenchymal transition (EMT) in vitro, and tumor progression and metastasis in vivo."

reach
"BAP1 suppresses the proliferation of some cell types."

reach
"BRCA1 Associated Protein-1 Suppresses Osteosarcoma Cell Proliferation and Migration Through Regulation PI3K and Akt Pathway."

sparser
"One intact allele of the BAP1 gene on chromosome 3 is requisite for the expression and translocation to the nucleus where BAP1 can inhibit tumor proliferation. xref Suppression of the nuclear localization of BAP1 (biallelic suppression) occurs in monosomy 3 because it is combined with assorted mutations on the remaining allele that may truncate the BAP1 protein, or affect nuclear localizer regions. xref BAP1 has definite additional prognostic value compared to the aforementioned tests and unlike the other prognostic tests permits microscopic confirmation of tumor."

reach
"As BAP1 can both promote and suppress cell proliferation based on the cell types, its ability to repress cellular growth in vitro does not necessarily correlate with its major tumor suppressor function in vivo [XREF_BIBR]."

reach
"Overexpression of wild-type but not mutant BAP1 significantly suppressed ICC cell proliferation, cell cycle progression, and invasion in vitro, as well as tumor progression in vivo."

reach
"XREF_BIBR showed that BAP1 overexpression in BAP1-null malignant pleural mesothelioma cell lines promoted cell proliferation and that BAP1 knockdown decreased proliferation in three malignant pleural mesothelioma cell lines."

reach
"In cutaneous melanoma cell lines, however, stable over-expression of BAP1 promoted cell growth while knockdown of BAP1 suppressed cell proliferation with concomitant decrease of survivin, an anti-apoptotic protein [17]."

eidos
"Our data suggest that BAP1 promotes cell proliferation and migration , and enhances the expression of KLF5 and its downstream genes , including CyclinD1 and FGF-BP1 , in the esophageal carcinoma cell line ECA109 ."

reach
"In both cell lines, knockdown of BAP1 significantly inhibited cell proliferation (P < 0.05; XREF_FIG and XREF_FIG) and clonogenic survival (XREF_FIG - XREF_FIG)."

reach
"In conclusion, BAP1 is a KLF5 DUB and BAP1 promotes basal breast cell proliferation, migration, tumour growth and metastasis partially through stabilizing KLF5 (XREF_SUPPLEMENTARY)."

reach
"BAP1 contributes to colon cancer cell proliferation by accelerating DNA replication and suppressing replication stress and concomitant apoptosis."

reach
"In summary, despite the findings implicating BAP1 in suppression of proliferation XREF_BIBR, XREF_BIBR, some groups have reported that BAP1 promotes cell proliferation XREF_BIBR, XREF_BIBR, XREF_BIBR."

reach
"These results confirmed that BAP1 promotes cell proliferation in vitro through KLF5 in part."

reach
"BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5."

reach
"Reintroduction of BAP1 into BAP1 deficient mesothelioma cells either had no effect or modestly increased cell proliferation."

reach
"In CMML, UTX mutations co-occurred with ASXL1, TET2, or CBL mutations, and in bladder cancer with somatic BAP1 mutations [75] and enhanced in vitro proliferation, in vivo tumor growth and cell migrati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In CMML, UTX mutations co-occurred with ASXL1, TET2, or CBL mutations, and in bladder cancer with somatic BAP1 mutations [XREF_BIBR] and enhance in vitro proliferation, in vivo tumor growth and cell migration [XREF_BIBR]."
BAP1 bound to ASXL1-MT activates cell population proliferation. 1 / 1
| 1

reach
"The ASXL1-MT and BAP1 complex enhances transient myeloid proliferation, but does not increase the long-term proliferation potential of murine and human HSPCs."
BRCA1 affects BAP1
4 1 | 24 45
4 1 | 23 38

sparser
"31,32 Because BAP1 can interact with BRCA1 and because BAP1FL reconstitution in BAP1-null cell lines leads to resistance to PARP inhibition (unlike in BAP1Δ-reconstituted ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"These data reveal that the interaction between BAP1 and BRCA1 is specific (BAP1 does not bind to any other RING finger domain tested to date) and suggests that the absence of BAP1 binding to mutat[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"BAP1-BRCA1 interaction is however still controversial [ xref ]."

sparser
"Although the direct interaction of BAP1 with BRCA1 remains controversial [ xref ], several studies report a potential role of BAP1 in DNA damage repair, and particularly in homologous recombination (HR)."

reach
"Although the molecular genetics of cutaneous melanomas have been investigated in numerous studies [XREF_BIBR, XREF_BIBR, XREF_BIBR], the genetic events that lead to the development of CBN, MABN or ACBN are poorly understood and are limited to a few genes [XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR], including BAP1 (BRCA1 binding protein 1), an oncogenic deubiquitinase, which has been found to be mutated in metastatic uveal melanomas and MABNs [XREF_BIBR]."

sparser
"This suggests that the effects of BAP1 on foci assembly could involve direct histone H2A-deubiquitination, although other mechanisms, for example involving the interaction of BAP1 with BRCA1 ( Jensen [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Several lines of evidences indicate that PARP inhibitor administration might be an effective treatment in presence of BAP1 and/or RAD21 alterations since both BAP1 and RAD21 are involved in the DNA repair pathway, BAP1 interacts with BRCA1 and BRCA1-mediated DNA repair pathway alterations enhance the sensitivity to PARP inhibitor administration."

sparser
"Because of the involvement of RAD21 in the DNA repair pathway, the interaction of BAP1 with BRCA1 and the enhanced sensitivity to PARP inhibitor administration in presence of alterations in the BRCA1-mediated DNA repair pathway, it was decided first to treat the patient with FOLFIRI every 2 weeks [irinotecan 180 mg/m 2 , folinic acid 400 mg/m 2 , 5-fluorouracil (5-FU) 400 mg/m 2 intravenous infusion bolus, then 5-FU 2400 mg/m 2 intravenous infusion over 46 h] and then to start a PARP inhibitor."

reach
"However, it is possible that BAP1 interacts with BRCA1 under a particular set of circumstances, such as in response to DNA damage."

sparser
"Thus, NUMB enhances Notch signaling by regulating the ubiquitinating activity of the BAP1BRCA1 complex."
BRCA1 binds BAP1 and BARD1. 6 / 6
| 1 5

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear and needs to be studied in more detail."

sparser
"BAP1 interacts with BARD1 and BRCA1 and inhibits the E3 ligase activity of BRCA1/BARD1."

sparser
"The ring domains of BRCA1 (residues 1-109) are associated with BARD1 [ xref ] and BAP1 [ xref ]."

sparser
"BAP1 and BRCA1-BARD1 complex coordinately regulate ubiquitination during the DNA damage response and the cell cycle [ xref , xref ]."

reach
"Until recently, the combination of pemetrexed and cisplatin was the only approved first-line therapy, yielding a median overall survival (OS) of 12.1 months.2 Nivolumab plus ipilimumab was approved recently as initial treatment for malignant pleural mesothelioma (MPM) on the basis of a phase 3 clinical trial that resulted in a median OS of 18.1 months.3 4 There are no approved therapies for patients with tumor relapse after first-line therapies.Risk factors in developing mesothelioma include exposure to asbestos5 and other carcinogenic fibers such as erionite,6 and previous radiation therapy.7 8 Studies of familial clusters of mesothelioma have revealed genetic predisposition to develop MM.9 10 Recent work has identified germline mutations in BAP1 that can predispose to mesothelioma11 12 and other cancers such as uveal and cutaneous melanoma, basal cell carcinoma, meningioma, and renal cell cancers.13 14 We previously identified deleterious germline mutations in 12% of 241 consecutive patients with MM who enrolled on a prospective natural history study, with BAP1 being the most frequent germline mutation.15 BAP1 was also recurrently inactivated at the somatic level, suggesting BAP1 variants undergoing positive selection in the context of the classic “two-hit” model.BAP1, a nuclear deubiquitylase, was initially discovered as an interaction partner of the tumor suppressor BRCA1.16 Subsequent studies revealed that BAP1 binds to BARD1, which, in turn, binds to BRCA1, forming the BRCA1-BARD1-BAP1 complex that is crucial for the homologous recombination (HR)–mediated repair of DNA double-strand breaks (DSBs).17 PARP enzymes play a major role in DNA single-strand break repair and base excision repair pathways."

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear."
BRCA1 binds BAP1 and RING. 2 / 2
| 2

sparser
"BAP1 interacts with the RING domain of human BRCA1, a predominately nuclear, ovarian and breast cancer susceptibility gene product with tumor suppressor activity."

sparser
"The BRCA1 RING domain also binds BAP1 (BRCA1-associated protein-1), a de-ubiquitinating enzyme 26 ."
BAP1 affects ASXL2
10 | 19 49
BAP1 binds ASXL2.
10 | 15 49
10 | 15 42

sparser
"Importantly, these new studies elucidate the molecular dynamics of these interactions, measure the kinetic and stoichiometric impact of mutations on protein binding and on the enzymatic activity of BAP1, and provide novel insights about the structural and dynamic parameters of the BAP1-ASXL2 interaction into single cell datasets that can inform future small-molecule approaches designed to reactivate latent wild-type UCH activity in BAP1 -mutant malignancies."

sparser
"Here, we show how these mutations affect BAP1 interactions with the Polycomb group-like protein ASXL2, using combinations of computational modeling technology, molecular biology, and in vitro reconstitution biochemistry."

reach
"Thus, we hypothesized that Ub binding by the BAP1/DEUBAD complex without actual catalysis is important for DEUBAD monoubiquitination and we sought to investigate this possibility."

sparser
"Combined computational and biochemical approaches demonstrate that the BAP1-ASXL2 interaction is direct and high affinity and that many BAP1 mutations act allosterically to inhibit BAP1-ASXL2 binding."

reach
"Purification of DEUBAD/BAP1 complexes."

No evidence text available

reach
"We demonstrate that interactions between BAP1 and ASXL2 are direct, specific, and stable to biochemical and biophysical manipulations as detected by isothermal titration calorimetry, GST association, and optical biosensor assays."

No evidence text available

sparser
"Because most ASXL2 mutations are out-of-frame frameshift mutations in exons 11 and 12, at least in AML with t (18;21) [ xref ], the BAP1 binding region in ASXL2 is also unaffected, although it remains to be determined whether truncated ASXL2-BAP1 complexes exhibit enhanced DUB activity."

reach
"These results suggest that BAP1, ASXL2, and UBE2Es expression are correlated in mesothelioma tissues and are in further support of the functional interaction between UBE2Es and the BAP1/ASXL2 complex."
ASXL1 binds ASXL2 and BAP1. 3 / 3
| 3

sparser
"Although both ASXL1 and ASXL2 have been identified to bind to BAP1, recent biochemical characterization of BAP1 complexes by xref suggest that BAP1 forms two mutually exclusive complexes with ASXL1 and ASXL2."

sparser
"Depletion of the PHD fingers did not affect the stability of ASXL1 or ASXL2, or the interaction between ASXL1, ASXL2, and BAP1 (Additional file xref : Fig. S2A, B)."

sparser
"Proteomic analysis of BAP1 interacting partners using this system revealed that BAP1 interacts with ASXL1 and ASXL2 in vivo as well as host cell factor 1 (HCF-1), O-GlcNAc transferase (OGT), and Forkhead box protein K1/2 (FOXK1/2) ( xref )."
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
BAP1 ubiquitinates ASXL2.
| 4
BAP1 leads to the ubiquitination of ASXL2. 4 / 4
| 4

reach
"Thus, BAP1 promotes the ubiquitination of its co-factor ASXL2 regulating its stability, and this in turn regulates the enzymatic activity and function of the DUB complex."

reach
"As BAP1 promotes ASXL2 monoubiquitination in vivo, we evaluated whether BAP1 with E2-conjugating enzymes are sufficient to direct this event in vitro."

reach
"BAP1 promotes ASXL2 monoubiquitination on its DEUBAD domain."

reach
"Our results, obtained from cell-based studies, suggest that BAP1 might either directly stimulate ASXL2 monoubiquitination or preserve monoubiquitinated ASXL2 from degradation."
ASXL2 affects BAP1
10 | 18 49
ASXL2 binds BAP1.
10 | 15 49
10 | 15 42

sparser
"Importantly, these new studies elucidate the molecular dynamics of these interactions, measure the kinetic and stoichiometric impact of mutations on protein binding and on the enzymatic activity of BAP1, and provide novel insights about the structural and dynamic parameters of the BAP1-ASXL2 interaction into single cell datasets that can inform future small-molecule approaches designed to reactivate latent wild-type UCH activity in BAP1 -mutant malignancies."

sparser
"Here, we show how these mutations affect BAP1 interactions with the Polycomb group-like protein ASXL2, using combinations of computational modeling technology, molecular biology, and in vitro reconstitution biochemistry."

reach
"Thus, we hypothesized that Ub binding by the BAP1/DEUBAD complex without actual catalysis is important for DEUBAD monoubiquitination and we sought to investigate this possibility."

sparser
"Combined computational and biochemical approaches demonstrate that the BAP1-ASXL2 interaction is direct and high affinity and that many BAP1 mutations act allosterically to inhibit BAP1-ASXL2 binding."

reach
"Purification of DEUBAD/BAP1 complexes."

No evidence text available

reach
"We demonstrate that interactions between BAP1 and ASXL2 are direct, specific, and stable to biochemical and biophysical manipulations as detected by isothermal titration calorimetry, GST association, and optical biosensor assays."

No evidence text available

sparser
"Because most ASXL2 mutations are out-of-frame frameshift mutations in exons 11 and 12, at least in AML with t (18;21) [ xref ], the BAP1 binding region in ASXL2 is also unaffected, although it remains to be determined whether truncated ASXL2-BAP1 complexes exhibit enhanced DUB activity."

reach
"These results suggest that BAP1, ASXL2, and UBE2Es expression are correlated in mesothelioma tissues and are in further support of the functional interaction between UBE2Es and the BAP1/ASXL2 complex."
ASXL1 binds ASXL2 and BAP1. 3 / 3
| 3

sparser
"Although both ASXL1 and ASXL2 have been identified to bind to BAP1, recent biochemical characterization of BAP1 complexes by xref suggest that BAP1 forms two mutually exclusive complexes with ASXL1 and ASXL2."

sparser
"Depletion of the PHD fingers did not affect the stability of ASXL1 or ASXL2, or the interaction between ASXL1, ASXL2, and BAP1 (Additional file xref : Fig. S2A, B)."

sparser
"Proteomic analysis of BAP1 interacting partners using this system revealed that BAP1 interacts with ASXL1 and ASXL2 in vivo as well as host cell factor 1 (HCF-1), O-GlcNAc transferase (OGT), and Forkhead box protein K1/2 (FOXK1/2) ( xref )."
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
ASXL2 activates BAP1.
| 3
ASXL2 activates BAP1. 3 / 3
| 3

reach
"BAP1 promotes DEUBAD monoubiquitination resulting in an increased stability of ASXL2, which in turn stimulates BAP1 DUB activity."

reach
"The DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing its affinity for ubiquitin."

reach
"In this paper we describe that similar to the homologous UCH-L5 and RPN13 DEU complex, the DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing BAP1 's affinity specifically for the ubiquitin in the substrate, through a combination of mild effects."
BAP1 affects SLC7A11
| 7 53
BAP1 decreases the amount of SLC7A11.
| 32
BAP1 decreases the amount of SLC7A11. 10 / 30
| 30

reach
"Studies have shown that BAP1 inhibits the expression of solute carrier family 7 member 11 (SLC7A11) by decreasing H2Aub occupancy on it."

reach
"SLC7A11 is an essential BAP1 target in human cancers, and BAP1 represses SLC7A11 expression via reducing H2Aub occupancy on SLC7A11 promoter in a deubiquitinating dependent manner [XREF_BIBR, XREF_BIBR]."

reach
"In this manner, BAP1 reduces SLC7A11 expression and cysteine uptake, leading lipid peroxidation upregulation and the ferroptosis induction (71)."

reach
"Although the precise mechanism by which BAP1 induces SLC7A11 repression needs further elucidation, it was proposed that BAP1, a H2A deubiquitinase, represses SLC7A11 expression by regulating the levels of H2A ubiquitination (H2Aub) on the SLC7A11 promoter (Figure 2) [41]."

reach
"Specifically, our study suggests a model in which the tumor suppressor BAP1 normally functions to repress the expression of SLC7A11 at least partly by deubiquitinating H2Aub on SLC7A11, thereby inhibiting cystine uptake into cells and rendering them more sensitive to ferroptosis."

reach
"Functional studies reveal that BAP1 decreases H2Aub occupancy on the SLC7A11 promoter and represses SLC7A11 expression in a deubiquitinating dependent manner, and that BAP1 inhibits cystine uptake by repressing SLC7A11 expression, leading to elevated lipid peroxidation and ferroptosis."

reach
"Epigenetically, BAP1 inhibits SLC7A11 expression through de-ubiquitinating H2A [13], while chromatin remodeling factor ARID1A increases SLC7A11 expression [14]."

reach
"In addition, erastin potently induced SLC7A11 expression in BAP1 deficient cells (XREF_SUPPLEMENTARY - XREF_SUPPLEMENTARY), suggesting that BAP1 represses the basal expression of SLC7A11 and that erastin induces SLC7A11 expression likely through BAP1 independent mechanisms."

reach
"BRCA1-associated protein 1 (BAP1) [18], the crucial constituent of the deubiquitinase complex, attenuated the initiation and extension of SLC7A11 transcription by deubiquitinating histone 2A, while the steady-state levels and half-life of SLC7A11 were improved by combination with the ovarian tumor family member deubiquitinase (OTUB1) [19]."

reach
"Both BAP1 and PRC1 (the main H2Aub ubiquitin ligase) inhibit the expression of SLC7A11 [XREF_BIBR]."
Modified BAP1 decreases the amount of SLC7A11. 2 / 2
| 2

reach
"We confirmed that re-expression of BAP1 WT, but not its C91A mutant, in UMRC6 cells decreased SLC7A11 expression (XREF_FIG), suggesting that BAP1 mediated repression of SLC7A11 expression requires BAP1 's DUB activity."

reach
"Similarly, BAP1 re-expression in NCI-H226 cells, a BAP1 deficient mesothelioma cell line with high SLC7A11 expression (see XREF_FIG), also repressed SLC7A11 expression (XREF_SUPPLEMENTARY - XREF_SUPPLEMENTARY)."
BAP1 inhibits SLC7A11.
| 7 11
| 7 11

reach
"It was reported that the BRCA1-associated protein 1 (BAP1) tumor suppressor is also able to promote ferroptosis by repressing SLC7A11 [41]."

eidos
"Further mechanistic studies demonstrated that BAP1 suppresses SLC7A11 expression partly by deubiquitinating histone 2A ubiquitination to promote ferroptosis ."

reach
"In addition, we studied how BAP1 coordinates with other transcription factors to regulate SLC7A11 expression and show that BAP1 mediated SLC7A11 repression does not require NRF2 and ATF4 transcription factors."

reach
"It has been recently reported that BAP1 facilitates lipid peroxidation and promotes ferroptosis through repressing SLC7A11 [24]."

reach
"Genomic analyses together with robust statistics have revealed that the restoration of BAP1 facilitates the formation of the polycomb repressive deubiquitinase (PR-DUB) complex and inhibits ubiquitinated histone 2A (H2Aub) occupancy on the SLC7A11 promoter."

eidos
"As a deubiquitinating enzyme , BAP1 inhibits the expression of SLC7A11 by reducing the ubiquitination of H2A to inhibit the activity of SLC7A11 promoter ( 23 ) ."

eidos
"Both BAP1 and PRC1 ( the main H2Aub ubiquitin ligase ) inhibit the expression of SLC7A11 [ 54 ] ."

reach
"Together, our data suggested a model that the BAP1 containing PR and DUB complex binds on the SLC7A11 promoter, where BAP1 removes ubiquitin from H2Aub, and BAP1 dependent H2Aub reduction on SLC7A11 is associated with BAP1 mediated SLC7A11 repression."

reach
"Several studies have revealed that BAP1 can inhibit ubiquitinated histone 2A (H2Aub) occupancy on the SLC7A11 promoter (Zhang et al., 2018)."

reach
"The knockdown of SLC7A11 in UMRC6 cells (a BAP1-deficient renal cancer cell line) marginally affects cancer cell proliferation, suggesting that the BAP1-mediated tumor suppression through regulating SLC7A11 [80]."
BAP1 increases the amount of SLC7A11.
| 8
BAP1 increases the amount of SLC7A11. 8 / 8
| 8

reach
"The tumor suppressor BRCA1 associated protein 1 (BAP1) represses SLC7A11 expression via reducing H2Aub occupancy on SLC7A11 promoter in a deubiquitinating dependent manner [XREF_BIBR]."

reach
"As a particular SMG of C1, BAP1 has been certified to block cystine uptake by inhibiting the expression of SLC7A11, leading to lipid peroxidation and ferroptosis, thereby inhibiting tumor progression."

reach
"As discussed in Boxes 2 and 3, inactivation of tumour suppressors such as p53, BAP1, kelch-like ECH associated protein 1 (KEAP1) and ARF (p14), or activation of oncogenic KRAS, upregulates SLC7A11 expression, which can be dependent or independent of nuclear factor erythroid 2-related factor 2 (NRF2), conferring ferroptosis evasion and promoting tumour growth ."

reach
"Functional studies reveal that BAP1 decreases H2Aub occupancy on the SLC7A11 promoter and represses SLC7A11 expression in a deubiquitinating dependent manner, and that BAP1 inhibits cystine uptake by repressing SLC7A11 expression, leading to elevated lipid peroxidation and ferroptosis."

reach
"The tumor suppressor BRCA1-associated protein 1 inhibits the growth of cancer cells by regulating the expression of SLC7A11 (Zhang et al., 2019), and its effect is similar to Erastin."

reach
"The tumor suppressor BAP1, an H2A deubiquitinating enzyme, can reduce SLC7A11 expression by inhibiting H2A ubiquitination (H2Aub) on the SLC7A11 promoter, thus controlling ferroptosis (Zhang Y.L."

reach
"Conversely, BAP1 deficiency by CRISPR technology increased SLC7A11 expression in several BAP1-proficient renal cancer cells with low SLC7A11 levels, including 786-O, Caki1, and ACHN cells (XREF_FIG - XREF_FIG and XREF_SUPPLEMENTARY - XREF_SUPPLEMENTARY)."

reach
"The tumor suppressor protein BRCA1-associated protein 1 (BAP1) inhibits SLC7A11 expression in a deubiquitinating-dependent manner and induces lipid peroxidation to promote ferroptosis (53)."
BAP1 ubiquitinates SLC7A11.
| 2
BAP1 ubiquitinates SLC7A11. 2 / 2
| 2

reach
"Specifically, our study suggests a model in which the tumor suppressor BAP1 normally functions to repress the expression of SLC7A11 at least partly by deubiquitinating H2Aub on SLC7A11, thereby inhibiting cystine uptake into cells and rendering them more sensitive to ferroptosis."

reach
"Regulation of H2A ubiquitination and SLC7A11 expression by BAP1 and PRC1."
BAP1 affects PBRM1
| 10 46
BAP1 binds PBRM1.
| 8 46
| 8 33

sparser
"Complementing the analysis of the clinical significance of mutations in PBRM1 and BAP1 in ccRCC, multiple groups, including ours, assessed the association of PBRM1 and BAP1 protein expression with clinical outcomes."

sparser
"Mutations in some of these genes, such as PBRM1 and BAP1 in RCC, are associated with poor patient outcome (Joseph et al ., xref ; Pawlowski et al ., xref ), indicating that their downstream pathways may also contribute to cancer progression and metastasis."

sparser
"Kaplan-Meier and Cox regression models were used to evaluate association of PBRM1 and BAP1 expression with the risk of death from renal cell carcinoma and the risk of metastasis after adjustment for age and the Mayo Clinic SSIGN (stage, size, grade and necrosis) score."

sparser
"Ongoing investigation is needed to determine the clinical significance of additional mutations in ccRCC and whether PBRM1 or BAP1 expression is associated with the response to currently available therapies for ccRCC."

sparser
"Gerlinger and colleagues have shown that intragenic VHL mutations and loss of 3p are the only uniformly truncal events in ccRCC.( xref ) We have shown that loss of PBRM1 and BAP1 are mutually exclusive and that loss of PBRM1 is associated with better outcomes than loss of BAP1.( xref , xref , xref ) Herein we validate previous results from a meta-analysis showing that PBRM1 and SETD2 mutations cooperate in ccRCC.( xref ) Specifically, we find that H3K36me3 loss is 3 times more likely in PBRM1-negative tumors than in those that express PBRM1."

sparser
"BAP1 and PBRM1 mutations are associated with characteristic and non-overlapping gene expression signatures, and outcomes for patients with ccRCCs mutated in BAP1 or PBRM1 are different [ xref ]."

sparser
"Germline mutations in BAP1 and PBRM1 have been associated with ccRCC familial syndromes, but these mutations are rare and BAP1 germline mutations are primarily associated with other tumor types such as melanoma and mesothelioma ( xref – xref )."

sparser
"The strong associations of PBRM1 and BAP1 expression with pathological and clinical outcomes further emphasize the biological importance of these genes for classifying ccRCC."

sparser
"With first-line everolimus, PBRM1 and BAP1 mutations were associated with longer (median [95% confidence interval {CI}] 12.8 [8.1, 18.4] vs 5.5 [3.1, 8.4] mo) and shorter (median [95% CI] 4.9 [2.9, 8.1] vs 10.5 [7.3, 12.9] mo) PFS1L, respectively."

sparser
"Finally and perhaps most importantly, despite the unknown biological rationale of the genetic interaction between PBRM1 and BAP1 we conclude that these data support the simultaneous analysis of PBRM1 and BAP1."
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
SETD2 binds PBRM1 and BAP1. 3 / 3
| 3

sparser
"The mutations in the chromatin regulators PBRM1 , BAP1 , and SETD2 were differentially associated with altered expression patterns of large numbers of genes when compared to samples bearing a background of VHL mutation ( xref , xref )."

sparser
"In fact this association with chromosome 3p loss has recently been strengthened by several publications demonstrating that 4 out of five ( VHL , PBRM1 , SETD2 and BAP1 ) of the most frequently mutated genes associated with CCRCC are on chromosome 3p xref – xref and that the mutation of either SETD2 , BAP1 or PBRM1 associates with poorer prognosis or progression in those patients xref , xref , xref ."

sparser
"On univariable analysis SETD2 (HR: 3.30), BAP1 (HR: 2.44) and PBRM1 (HR: 1.78) were significantly associated with a higher risk of metastases."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
PBRM1 binds BAP1 and H3K36me3. 2 / 2
| 2

sparser
"We previously evaluated the prognostic impact of BAP1 and PBRM1 to define epigenetic subtypes of clear cell renal cell carcinoma.( xref , xref ) To examine the association of H3K36me3 with BAP1 and PBRM1 protein expression, we evaluated the expression of all 3 markers dichotomized as positive versus negative in clear cell renal cell carcinoma samples with available staining ( xref )."

sparser
"Association of H3K36me3 with Epigenetic Tumor Suppressors BAP1 and PBRM1."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
BAP1 activates PBRM1.
| 2
BAP1 activates PBRM1. 2 / 2
| 2

reach
"Interestingly, BAP1 loss reduced the protein levels of STAT2 and IRF9 independent of PBRM1 status, since RCC4 cells express no endogenous PBRM1 (XREF_FIG)."

reach
"XREF_BIBR, XREF_BIBR Together these findings led us to propose a model where following inactivation of VHL and 3p loss, inactivation of the remaining copy of BAP1 caused aggressive ccRCC and inactivation of PBRM1, less aggressive ccRCC."
PBRM1 affects BAP1
| 9 46
PBRM1 binds BAP1.
| 8 46
| 8 33

sparser
"Complementing the analysis of the clinical significance of mutations in PBRM1 and BAP1 in ccRCC, multiple groups, including ours, assessed the association of PBRM1 and BAP1 protein expression with clinical outcomes."

sparser
"Mutations in some of these genes, such as PBRM1 and BAP1 in RCC, are associated with poor patient outcome (Joseph et al ., xref ; Pawlowski et al ., xref ), indicating that their downstream pathways may also contribute to cancer progression and metastasis."

sparser
"Kaplan-Meier and Cox regression models were used to evaluate association of PBRM1 and BAP1 expression with the risk of death from renal cell carcinoma and the risk of metastasis after adjustment for age and the Mayo Clinic SSIGN (stage, size, grade and necrosis) score."

sparser
"Ongoing investigation is needed to determine the clinical significance of additional mutations in ccRCC and whether PBRM1 or BAP1 expression is associated with the response to currently available therapies for ccRCC."

sparser
"Gerlinger and colleagues have shown that intragenic VHL mutations and loss of 3p are the only uniformly truncal events in ccRCC.( xref ) We have shown that loss of PBRM1 and BAP1 are mutually exclusive and that loss of PBRM1 is associated with better outcomes than loss of BAP1.( xref , xref , xref ) Herein we validate previous results from a meta-analysis showing that PBRM1 and SETD2 mutations cooperate in ccRCC.( xref ) Specifically, we find that H3K36me3 loss is 3 times more likely in PBRM1-negative tumors than in those that express PBRM1."

sparser
"BAP1 and PBRM1 mutations are associated with characteristic and non-overlapping gene expression signatures, and outcomes for patients with ccRCCs mutated in BAP1 or PBRM1 are different [ xref ]."

sparser
"Germline mutations in BAP1 and PBRM1 have been associated with ccRCC familial syndromes, but these mutations are rare and BAP1 germline mutations are primarily associated with other tumor types such as melanoma and mesothelioma ( xref – xref )."

sparser
"The strong associations of PBRM1 and BAP1 expression with pathological and clinical outcomes further emphasize the biological importance of these genes for classifying ccRCC."

sparser
"With first-line everolimus, PBRM1 and BAP1 mutations were associated with longer (median [95% confidence interval {CI}] 12.8 [8.1, 18.4] vs 5.5 [3.1, 8.4] mo) and shorter (median [95% CI] 4.9 [2.9, 8.1] vs 10.5 [7.3, 12.9] mo) PFS1L, respectively."

sparser
"Finally and perhaps most importantly, despite the unknown biological rationale of the genetic interaction between PBRM1 and BAP1 we conclude that these data support the simultaneous analysis of PBRM1 and BAP1."
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
SETD2 binds PBRM1 and BAP1. 3 / 3
| 3

sparser
"The mutations in the chromatin regulators PBRM1 , BAP1 , and SETD2 were differentially associated with altered expression patterns of large numbers of genes when compared to samples bearing a background of VHL mutation ( xref , xref )."

sparser
"In fact this association with chromosome 3p loss has recently been strengthened by several publications demonstrating that 4 out of five ( VHL , PBRM1 , SETD2 and BAP1 ) of the most frequently mutated genes associated with CCRCC are on chromosome 3p xref – xref and that the mutation of either SETD2 , BAP1 or PBRM1 associates with poorer prognosis or progression in those patients xref , xref , xref ."

sparser
"On univariable analysis SETD2 (HR: 3.30), BAP1 (HR: 2.44) and PBRM1 (HR: 1.78) were significantly associated with a higher risk of metastases."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
PBRM1 binds BAP1 and H3K36me3. 2 / 2
| 2

sparser
"We previously evaluated the prognostic impact of BAP1 and PBRM1 to define epigenetic subtypes of clear cell renal cell carcinoma.( xref , xref ) To examine the association of H3K36me3 with BAP1 and PBRM1 protein expression, we evaluated the expression of all 3 markers dichotomized as positive versus negative in clear cell renal cell carcinoma samples with available staining ( xref )."

sparser
"Association of H3K36me3 with Epigenetic Tumor Suppressors BAP1 and PBRM1."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
PBRM1 inhibits BAP1.
| 1
PBRM1 inhibits BAP1. 1 / 1
| 1

reach
"Genetic markers of ccRCC include biallelic inactivation of Von Hippel-Lindau (VHL) tumor suppressor genes; negative regulators of hypoxia inducible factor (HIF) protein; copy number changes of chromosome 3p, 5q, and 14q genes; and chromatin modifying enzyme high mutation frequencies, such as protein polybromo-1 (PBRM1), SET domain containing 2 (SETD2), and BRCA1 associated protein-1 (BAP1) [XREF_BIBR]."
BAP1 affects Neoplasms
| 3 33
BAP1 inhibits Neoplasms.
| 3 22
| 3 22

reach
"BAP1-inactivated melanocytic tumors represent a subset of epithelioid melanocytic neoplasms resulting from biallelic inactivation of the BAP1 gene and by a driver mutation that activate the MAP kinase pathway, most commonly BRAFV600E."

reach
"Fusions involved known tumor suppressors BAP1, MTAP, and LRP1B, and a clonal oncogenic fusion involving CACNA1D-ERC2, PARD3B-NT5DC2, and STAB2-NT5DC2 fusions were also identified as clonal fusions."

eidos
"Zhang et al. showed that BAP1 inhibits tumor progression by promoting cellular ferroptosis ( 15 ) ."

reach
"BINs are highly penetrant, present in up to 90% of mutation carriers, and typically present as multiple, skin colored or reddish-brown, dome-shaped melanocytic tumors (also or previously called BAP1-inactivlated melanocytic tumors, Wiesner nevi, BAPomas, nevoid melanoma-like melanocytic proliferations, BAP1 mutant Spitz nevi, and melanocytic BAP1-mutated atypical intradermal tumors)."

reach
"Multiple Flesh-Colored Nodules: A Patient with Multiple BAP 1-Inactivated Melanocytic Tumors."

eidos
"In vivo results using a chicken embryo choriollantoic membrane ( CAM ) assay showed that BaP1 inhibits tumor growth , angiogenesis , and tumor proliferation ."

reach
"As for MPM, next-generation sequencing of 216 MPM patients showed that the tumor suppressors BAP1, NF2, and SETD2 were significantly mutated through gene fusions and splicing alterations (Bueno et al., 2015)."

reach
"These cases demonstrate the importance of routine BAP1 tumor testing in meningioma with rhabdoid features regardless of grade, germline testing for patients with BAP1 inactivated tumors, and tailored cancer screening in this population."

reach
"Zhang et al. showed that BAP1 inhibits tumor progression by promoting cellular ferroptosis (15)."

reach
"Germline mutations in BAP1 predispose carriers to the BAP1 tumor predisposition syndrome (BAP1-TPDS), characterized by BAP1-inactivated nevi (BINs), uveal melanoma, cutaneous melanoma, mesothelioma, renal cell carcinoma, and other tumors."
BAP1 activates Neoplasms.
| 11
| 8

reach
"Germline or somatic mutations and deletions of BAP1 have been identified in various tumor types, and downregulation or inactivation of BAP1 could accelerate tumor onset, invasion, recurrence, and metastases ."

reach
"This raises the possibility for genetic cooperation with BAP1-driven pathogenicity in tumor B, but this particular mutation has not been previously noted to be pathogenic.At present, more than 7 years post- resection, Patient A has no clinical or radiographic evidence of newly recurrent or progressive disease."

reach
"Whereas the tumor suppressive role has been attributed to BAP1’s important involvement in DNA double strand breakage repair [37], there is emerging evidence that BAP1 can also promote tumor growth when it is overexpressed in particular molecular environments."

reach
"Furthermore, BAP1 germline mutations cause a tumor predisposition syndrome inherited in an autosomal dominant pattern, which confers a higher risk for developing a spectrum of benign and malignant tumors , including pancreatic cancer ."

reach
"Through a biochemical screen, we identified BAP1 catalytic inhibitors that inhibit truncated-ASXL1-driven leukemic gene expression and impair tumor progression in vivo."

reach
"Pathogenic germline variants in the BRCA1-associated protein 1 (BAP1) gene cause a dominantly inherited tumor predisposition syndrome, BAP1-TPDS, in which MM is the second most common associated cancer."

reach
"The findings are in accordance with previous reports that reduced expression or inactivation of BAP1 in tumors, which often results from germline-inactivating or somatic-inactivating BAP1 mutations or deletions, increases tumor susceptibility, or predicts worse clinical outcomes ."

reach
"Here, we show that BAP1 is upregulated in colon cancer cells and tissues and that BAP1 depletion reduces colon cancer cell proliferation and tumor growth."
Mutated BAP1 activates Neoplasms. 3 / 3
| 3

reach
"Moreover, BAP1 mutations may elevate tumor responsiveness to immunotherapy (36)."

reach
"For example, mesothelioma cells that carry germline BAP1 mutations have upregulated aerobic glycolysis (17), and BAP1 mutant ccRCC tumor samples display elevated glycolysis (22)."

reach
"Cutaneous melanoma, melanocytic tumors and other skin tumors:. The frequency of cutaneous melanoma (CM) development is likely to increase with germline BAP1 mutations and tumor-promoting effects of these BAP1 mutations are strongly influenced by co-occurrence of associated oncogenes such as mutant BRAF (2,43)."
BAP1 affects KLF5
5 1 | 26 6
BAP1 binds KLF5.
5 | 9 6
5 | 9 6

No evidence text available

No evidence text available

reach
"BAP1 interacts directly with KLF5 and stabilizes KLF5 via deubiquitination."

reach
"Because KLF5 interacted with BAP1 directly, we wondered whether KLF5 was in the BAP1 and HCF -1 complex."

sparser
"Another KLF5 DUB that stood out in siRNA screening was BAP1 (BRCA1-associated protein 1), which also directly binds KLF5 and stabilizes KLF5 through deubiquitination [ xref ]."

reach
"BAP1 interacts directly with KLF5."

sparser
"BAP1 interacts directly with KLF5 and stabilizes KLF5 via deubiquitination."

sparser
"The deubiquitinase (DUB) BAP1 had been reported to regulate KLF5 stability in breast cancer cells ( xref , xref ), so we hypothesize that LINC00152 mediates KLF5 depending on the binding of BAP1 and KLF5."

No evidence text available

sparser
"As shown in xref , LINC00152 knockdown inhibits BAP1 binding with KLF5."
BAP1 deubiquitinates KLF5.
1 | 6
BAP1 deubiquitinates KLF5. 7 / 7
1 | 6

reach
"Compared with GST and BAP1-C91S, BAP1 specifically decreased KLF5 polyubiquitination (XREF_FIG; XREF_SUPPLEMENTARY)."

"BAP1 interacts directly with KLF5 and stabilizes KLF5 via deubiquitination."

reach
"In breast cancer cell lines, BAP1 plays an oncogenic function by directly deubiquitinating and stabilizing KLF5 (Kruppel-like factor 5)."

reach
"BAP1, but not BAP1-C91S, markedly decreased KLF5 protein polyubiquitination (XREF_FIG, lanes 3 and 4), whereas another DUB, A20, did not decrease KLF5 polyubiquitination (XREF_FIG, lane 5)."

reach
"Second, BAP1 decreases KLF5 polyubiquitination and increases KLF5 protein stability in a DUB activity dependent manner."

reach
"BAP1 antagonizes WWP1 mediated transcription factor KLF5 ubiquitination and inhibits autophagy to promote melanoma progression."

reach
"Finally, we demonstrated that knockdown of endogenous BAP1 increased the ubiquitination of endogenous KLF5 in HCC1806 (XREF_FIG)."
BAP1 activates KLF5.
| 7
BAP1 activates KLF5. 7 / 7
| 7

reach
"To test whether BAP1 can antagonize E3 ligase mediated KLF5 degradation, we transfected KLF5 E3 ligases and BAP1 into HEK293FT cells and detected the KLF5 protein levels."

reach
"To further confirm whether BAP1 increases KLF5 protein stability, we transfected two different siRNAs targeting different regions of BAP1 into MCF10A and breast cancer cell lines (HCC1806 and HCC1937)."

reach
"Taken together, these results demonstrate that BAP1 promotes HCC1806 breast cancer tumorigenesis partially by stabilizing KLF5."

reach
"However, BAP1 positively regulates KLF5 at the protein level."

reach
"Collectively, these results suggest that BAP1 is a specific DUB for KLF5 and that BAP1 increases KLF5 protein stability in a DUB activity dependent manner."

reach
"These results indicate that BAP1 promotes KLF5 positive breast cell proliferation in vitro."

reach
"Besides, BAP1 promoted KLF5 mediated growth of melanoma in vivo."
BAP1 increases the amount of KLF5.
| 4
BAP1 increases the amount of KLF5. 4 / 4
| 4

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"BAP1 knockdown decreased the endogenous protein levels of KLF5 and its downstream target gene FGF-BP XREF_BIBR (XREF_FIG)."

reach
"The KLF5 mRNA levels were not decreased by BAP1 knockdown in the MCF10A and HCC1806 cells (XREF_SUPPLEMENTARY)."

reach
"Our data suggest that BAP1 promotes cell proliferation and migration, and enhances the expression of KLF5 and its downstream genes, including CyclinD1 and FGF-BP1, in the esophageal carcinoma cell line ECA109."

reach
"In addition, expression of KLF5, CyclinD1, and FGF-BP1 was increased by BAP1 overexpression and decreased by BAP1 knockdown."
IHC affects BAP1
| 37
IHC increases the amount of BAP1.
| 13
IHC increases the amount of BAP1. 10 / 13
| 13

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"Bap1 protein expression by IHC was reduced across lesions and completely absent in approximately 30% (XREF_FIG)."

reach
"As anticipated, we identified a significant association of p16 loss with poor outcomes that was independent of histologic subtype, and as previously reported, that BAP1 expression by IHC is not an ind[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Our observation that a greater number of tumors exhibited loss of BAP1 protein expression by IHC compared with BAP1 deletions or mutations indicates that BAP1 may become functionally inactivated by mechanisms other than deletions or mutations in the coding region; for example, epigenetic changes leading to silencing of the BAP1 gene or other, yet to be identified, alterations that prevent BAP1 expression."

reach
"2.2 PBRM1 and BAP1 Protein Expression by IHC."

reach
"Assessment of BAP1 protein expression by IHC has been shown to be an excellent surrogate for the functional status of BAP1.4,6 Loss of IHC expression correlates with biallelic inactivation of BAP1."

reach
"We previously demonstrated that BAP1 and PBRM1 expression by immunohistochemistry (IHC) are highly correlated with mutation status (P = 3E-58 and 4E-23 respectively 34 and that BAP1 and PBRM1 expression by IHC are independent predictors of both disease-free survival and overall survival in the localized disease setting."

reach
"Loss of BAP1 Expression by IHC Identifies a Morphologically Distinct Subset of Tumors."

reach
"These data are consistent with those of XREF_BIBR) for both patient survival based on the BAP1 mutational status (32 vs 133 months for BAP1 mutation positive vs BAP1 mutation negative tumours) or BAP1 protein expression (31 vs 133 months for tumours showing negative BAP1 expression by IHC vs those with positive BAP1 expression) and thus the proposed role of BAP1 in the pathogenesis of UM with an aggressive phenotype."

reach
"XREF_BIBR, XREF_BIBR Loss of PBRM1 or BAP1 expression by IHC is associated with a worse RCC specific survival in ccRCC supporting a role for distinguishing molecular subtypes of ccRCC."

reach
"In absence of invasion, loss of BAP1 expression by IHC and/or the presence of a homozygous deletion of CDKN2A (p16) by FISH or by methylthioadenosine phosphorylase (MTAP) IHC allow a differential diagnosis between neoplastic and non-neoplastic cells (16-18)."
IHC inhibits BAP1.
| 12
IHC inhibits BAP1. 10 / 12
| 12

reach
"Detection of BAP1 loss by IHC has been recognized as highly specific for MM, although the sensitivity of BAP1 loss is relatively low at approximately 50% in epithelioid type of MM and much lower in sa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"There was a significant association between BRAF mutation status and loss of BAP1 by IHC."

reach
"BAP1 loss by IHC was observed in 76% (29/38), including five wildtype on NGS."

reach
"These findings are comparable, as loss of BAP1 by IHC has been shown to correlate well with biallelic inactivation of BAP1.9 Although BAP1 loss was seen in only a small proportion (5%) of melanomas ov[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Furthermore, our results suggested that BAP1 might be functionally inactivated in 44% of ESCC by IHC analysis, because BAP1 has been reported to function within the nucleus and BAP1 was not expressed within the nucleus in 44% of ESCCs."

reach
"To our surprise, none of the sarcomatoid cases in our study demonstrated BAP1 loss; and perhaps more surprisingly, there were multiple sarcomatoid-predominant biphasic cases that did demonstrate loss [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Long-term follow-up was not available for these series [5,11]; thus, the question remains as to whether these tumors are atypical Spitz tumors or sporadic BAP1 associated melanocytic lesions.In summar[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The most commonly referred to markers in malignant mesothelioma are loss of BAP1 by IHC, and the deletion of p16 by FISH."

reach
"In several studies, the loss of BAP1 by IHC and the homozygous deletion of p16 by FISH is very specific for mesothelioma but still may lack sensitivity to a certain extent."

reach
"Another issue is that most mesotheliomas of peritoneal origin do show a loss of BAP1 by IHC but do not show loss of p16 by FISH."
IHC decreases the amount of BAP1.
| 10
IHC decreases the amount of BAP1. 10 / 10
| 10

reach
"In this pilot study, we inves- tigated whether loss of BAP1 expression by IHC is associated with prognosis in uveal melanoma patients."

reach
"In absence of invasion, loss of BAP1 expression by IHC and/or the presence of a homozygous deletion of CDKN2A (p16) by FISH or by methylthioadenosine phosphorylase (MTAP) IHC allow a differential diagnosis between neoplastic and non-neoplastic cells (16-18)."

reach
"In the current pilot study, we explored the hypothesis that loss of BAP1 expression by IHC is associated with prognosis in patients with uveal melanoma."

reach
"Both the naevus and melanoma components showed loss of BAP1 expression by IHC, XREF_BIBR suggesting that some BAP1 mutated epithelioid melanocytic tumors may progress to melanoma."

reach
"Here we report loss of BAP1 expression by IHC in 61% of MPM cases overall, with 77% of epithelioid cases demonstrating BAP1 loss and retention in all of the sarcomatoid cases examined."

reach
"XREF_BIBR, XREF_BIBR, XREF_BIBR Given that real rate of germline BAP1 mutations is likely to be less than 1%, it is reasonable that formal genetic testing for BAP1 mutation be reserved for those patients who are considered high-risk based on family and personal history and demonstrate loss of BAP1 expression by IHC."

reach
"Our observation that a greater number of tumors exhibited loss of BAP1 protein expression by IHC compared with BAP1 deletions or mutations indicates that BAP1 may become functionally inactivated by mechanisms other than deletions or mutations in the coding region; for example, epigenetic changes leading to silencing of the BAP1 gene or other, yet to be identified, alterations that prevent BAP1 expression."

reach
"The melanomas that have been tested show biallelic BAP1 inactivation.34 One of the melanomas described in this setting arose in association with an epithelioid naevus (similar to those described above[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"XREF_BIBR, XREF_BIBR Loss of PBRM1 or BAP1 expression by IHC is associated with a worse RCC specific survival in ccRCC supporting a role for distinguishing molecular subtypes of ccRCC."

reach
"Loss of BAP1 Expression by IHC Identifies a Morphologically Distinct Subset of Tumors."
IHC activates BAP1.
| 2
IHC activates BAP1. 2 / 2
| 2

reach
"FISH for p16 and CDKN2A deletion and loss of BAP1 by IHC are useful tests for confirming the diagnosis of MM."

reach
"[XREF_BIBR] The finding of homozygous deletion of p16 by FISH or loss of BAP1 by IHC can be very useful diagnostic tools in differentiating benign mesothelial proliferation from MM."
BAP1 affects FOXK2
11 1 | 10 15
BAP1 binds FOXK2.
11 | 6 15
11 | 6 14

No evidence text available

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sparser
"Furthermore, treatment of cells with the CDK inhibitor alsterpaullone had little effect on FOXK2BAP1 interactions (Supplementary Figure S2C)."

sparser
"It therefore appears likely that the R58A mutation does more than affect phospho-dependent interactions but underscores the importance of this domain in mediating interactions between FOXK2 and BAP1."

sparser
"Functional interactions between FOXK2 and BAP1 in target locus regulation."

No evidence text available

No evidence text available

sparser
"We also tested whether there was a reciprocal effect on FOXK2 binding upon BAP1 depletion but little effect was seen on the ChIP signal for FOXK2 following reductions in BAP1 expression levels by siRNA treatment (Figure xref ; Supplementary Figure S6B)."
FOXK2 binds PGR and BAP1. 1 / 1
| 1

sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
BAP1 inhibits FOXK2.
| 3
BAP1 inhibits FOXK2. 3 / 3
| 3

reach
"Moreover, BAP1 is part of a ternary complex bridging FOXK2 and HCF1 and represses FOXK2 target genes, an effect requiring BAP1 DUB activity but not the interaction with HCF1 binding."

reach
"BAP1 represses FoxK2 target genes, and this effect requires BAP1 DUB activity but not interaction with HCF-1."

reach
"Loss of BAP1 causes the increase of FoxK2 target genes, which is dependent on the Ring1B and Bmi1 complex."
BAP1 deubiquitinates FOXK2.
1 |
BAP1 deubiquitinates FOXK2. 1 / 1
1 |

"BRCA1-associated protein 1 (BAP1) deubiquitinase antagonizes the ubiquitin-mediated activation of FoxK2 target genes. Okino Y(1), Machida Y(1), Frankland-Searby S(2), Machida YJ(3). Author information: (1)From the Departments of Oncology and. (2)Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905. (3)From the Departments of Oncology and Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, Minnesota 55905 machida.yuichi@mayo.edu. BRCA1-associated protein 1 (BAP1), which is frequently mutated in cancer, functions as a deubiquitinase (DUB) for histone H2A.BAP1 represses FoxK2 target genes, and this effect requires BAP1 DUB activity but not interaction with HCF-1"
BAP1 activates FOXK2.
| 1
BAP1 activates FOXK2. 1 / 1
| 1

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"Importantly, BAP1 depletion causes up-regulation of FoxK2 target genes only in the presence of the Ring1B and Bmi1 complex, an E3 ubiquitin ligase for histone H2A, indicating an antagonizing role of BAP1 against Ring1B-Bmi1."
| 35
| 20

reach
"Furthermore, BAP1 knockdown inhibits tumorigenicity and lung metastasis, which can be rescued partially by ectopic expression of KLF5."

reach
"Finally, we wondered whether BAP1 and KLF5 promoted metastasis in vivo."

reach
"In conclusion, BAP1 is a KLF5 DUB and BAP1 promotes basal breast cell proliferation, migration, tumour growth and metastasis partially through stabilizing KLF5 (XREF_SUPPLEMENTARY)."

reach
"BAP1 promotes breast cancer cell proliferation and metastasis by deubiquitinating KLF5."

reach
"BAP1 and KLF5 promote breast cancer migration, invasion and metastasis may not through p27; however, KLF5 is a transcription factor regulating numerous downstream target genes, such as FGF-BP and mPGES1."

reach
"Nevertheless, our results suggest that BAP1 promotes breast tumour growth and metastasis partially through stabilizing KLF5."

reach
"BAP1 and KLF5 promote breast cancer lung metastasis."

reach
"Therefore, these findings clearly indicate that BAP1 promotes breast cancer lung metastasis partially through stabilizing KLF5."

reach
"Furthermore, BAP1 loss abrogates tumor growth and lung metastasis in murine syngeneic tumor models."

reach
"Germline or somatic mutations and deletions of BAP1 have been identified in various tumor types, and downregulation or inactivation of BAP1 could accelerate tumor onset, invasion, recurrence, and metastases ."
Mutated BAP1 activates Neoplasm Metastasis. 3 / 3
| 3

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"XREF_BIBR Therefore, both somatic mutation of BAP1 in primary UM tumors and germline mutations increase the risk of metastasis and impart low survival rate."

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"Germline BAP1 mutations increased risk of metastasis independent of ciliary body involvement (P =.02)."

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"37 Therefore, both somatic mutation of BAP1 in primary UM tumors and germline mutations increase the risk of metastasis and impart low survival rate."
| 15

reach
"The strong association between BAP1 and HIF1α might determine why BAP1 loss increases the risk of metastasis in certain cancer types such UM."

reach
"Finally, to elucidate the mechanism by which BAP1 suppresses invasion and metastasis in LAC cells, we analyzed the expression levels of metastasis related proteins in transfected H1299 cells and H1650 cells using IB, respectively."

reach
"We find that this process requires VCAM mediated adhesion between UM cells and ECs and that loss of the metastasis suppressor BAP1 enhances TEM."

reach
"BAP1 promotes progenitor cell differentiation into melanocytes by increasing MITF expression and BAP1 loss may promote metastasis in UM by remodeling the epigenome to resemble that of stem-like migratory neural crest cells."

reach
"Knockdown of BAP1 in TNBC cells inhibited tumorigenesis and metastasis, which could be partially rescued by restoration of KLF5 expression, suggesting that KLF5 mediates, at least in part, the breast cancer promoting function of BAP1 [XREF_BIBR]."

reach
"Prompted by these transcriptomic findings, we wished to explore further the possibility that BAP1 inhibits metastasis of uveal melanoma cells by maintaining their differentiated state and impeding their reversion to a stem like state."

reach
"The authors concluded that loss of BAP1 might increase susceptibility to uveal melanoma metastasis and serve as a valuable therapeutic target."

reach
"Our results reveal a novel route of transendothelial migration for uveal melanoma cells, and they provide insight into the mechanism by which loss of BAP1 promotes metastasis."

reach
"XREF_BIBR, XREF_BIBR The precise molecular explanation for why loss of BAP1 leads to metastasis in UM remains unclear."

reach
"19 However, GNA11 mutations are also more common in tumors involving the ciliary body, which is an independent risk factor for metastasis, and in tumors with mutations in the metastasis suppressor BAP1 (Breast cancer 1, early onset (BRCA1)-associated protein 1) (see below), so it remains possible or even likely that the association between GNA11 and metastasis is not causal."
| 2 29
BAP1 activates apoptotic process.
| 1 25
| 1 25

reach
"For example, BAP1 can enhance progression through the G1-S checkpoint and subsequently induce cell death by a process with similarities to both apoptosis and necrosis [XREF_BIBR]."

reach
"In the cytoplasm, BAP1 interacts with type 3 inositol-1,4,5-trisphosphate receptor (IP3R3) at the endoplasmic reticulum and promotes Ca2+ signaling and apoptosis in response to stress ."

reach
"Likewise, A549 cells transfected with the BAP1 siRNA showed suppression of cell apoptosis (XREF_SUPPLEMENTARY), whereas overexpression of BAP1 significantly promoted cell apoptosis (XREF_SUPPLEMENTARY)."

reach
"It is also prudent to note that expression of BAP1 increased the population of cells that stain positive for both AnnexinV and PI, an indication of late apoptosis and/or necrosis."

reach
"As a deubiquitinating enzyme, BAP1 contributes to gene transcription, cell differentiation, DNA damage repair, apoptosis, and cell metabolism in tumor inhibition."

reach
"However, the reduced amount of BAP1 in the cytoplasm of BAP1 cells impairs apoptosis and allows survival of cells that have acquired oncogenic mutations."

reach
"BAP1 downregulation by siRNA inhibited apoptosis induced by the combined treatment of ODN and oxaliplatin/etoposide."

reach
"In functional experiments, BAP1 has been shown to suppress lung cancer tumorigenesis in athymic nude mice, promote the cell cycle and induce both apoptosis and necrosis."

reach
"We show that BAP1 inactivation causes apoptosis in mouse embryonic stem cells, fibroblasts, liver, and pancreatic tissue but not in melanocytes and mesothelial cells."

reach
"It was recently implicated as the target of the tumor suppressor BAP1 that triggers apoptosis following exposure to genotoxic stress."
| 1 4

reach
"For instance, while loss of Bap1 activates intrinsic apoptosis in several mouse cell types (hepatocytes, keratinocytes, fibroblasts, and embryonic stem cells) in an RNF2 dependent fashion, the Bap1 loss enhances proliferation of melanocytes in association with upregulation of lineage specific oncogenes MITF and BCL2, independently of RNF2."

eidos
"Thus , loss of BAP1 activity prevents Bcl2 and Mcl1 expression and triggers apoptosis ."

reach
"Since both BAP1 and PTEN inhibit apoptosis in a similar way, increasing the likelihood for pre-malignant cells to survive, it is tempting to speculate that also PTEN mutations may contribute to mesothelioma development in carriers of PTEN mutations, as demonstrated in animal models carrying germline BAP1 mutations with even minimal exposure to asbestos [30]."

reach
"The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death."

reach
"In RCC cells, BAP1 inhibited glucose deprivation-induced apoptosis through interaction with the activating transcription factor 3 (ATF3) and DNA damage-inducible transcript 3 protein (DDIT3) gene promoters in a polycomb complex protein BMI-1-dependent manner ."
UBE2O affects BAP1
5 | 18 6
UBE2O ubiquitinates BAP1.
| 12 5
UBE2O ubiquitinates BAP1. 10 / 17
| 12 5

sparser
"Strikingly, UBE2O ubiquitinates BAP1, and modified forms were more abundant in the C91S mutant ( Figure 1 C)."

reach
"In addition, UBE2O mediated multi monoubiquitylation of BAP1 sequesters this DUB to the cytoplasm during adipocyte differentiation [XREF_BIBR]."

reach
"Ubiquitination of BAP1 by UBE2O results in sequestration of BAP1 in the cytoplasm, rendering it unable to find its nuclear targets."
| PMC

sparser
"Ubiquitination of BAP1 by UBE2O results in sequestration of BAP1 in the cytoplasm, rendering it unable to find its nuclear targets."
| PMC

reach
"UBE2O can also ubiquitinate the tumor-suppressive DUB BAP1 and regulate its subcellular localization and function in chromatin remodeling."

sparser
"UBE2O can also ubiquitinate the tumor-suppressive DUB BAP1 and regulate its subcellular localization and function in chromatin remodeling ( xref )."

reach
"While ubiquitination enzyme UBE2O monoubiquitinates the NLS of BAP1 and induces translocation (inactivation) of BAP1 from the nucleus to the cytoplasm, DUB activity of the UCH-domain of BAP1 counteracts the UBE2O activity mediated through the intramolecular interaction between the UCH-domain and COOH-terminal domain of BAP1."

sparser
"Interaction with UBE2O is also lost, this corroborates with the UBE2O interaction site that is mapped to the C-terminus of BAP1 and that ubiquitination of BAP1 by UBE2O requires its NLS [ xref ]."
| PMC

reach
"For example, UBE2O mediates multiple mono-ubiquitination of BAP1, a nuclear localization signal, and leads to its cytoplasmic sequestration [XREF_BIBR]."

sparser
"To further confirm that autodeubiquitination prevents accumulation of ubiquitin on BAP1, the bead-bound wild-type complex was pretreated with N-ethylmaleimide (NEM), which resulted in a significant ub[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
UBE2O activates BAP1.
| 5
UBE2O activates BAP1. 5 / 8
| 5

reach
"UBE2O-mediated ubiquitylation causes BAP1 to accumulate in the cytoplasm, whereas BAP1 autodeubiquitylation promotes nuclear retention."

reach
"The ubiquitin conjugating enzyme UBE2O induces BAP1 sequestration in the cytoplasm by multi monoubiquitylation of its nuclear localization signal (NLS)."

reach
"Indeed, the subcellular localization of BAP1 is coordinated by the E3 ligase UBE2O which promotes BAP1 cytoplasmic retention ."

reach
"The antagonistic relationship between UBE2O and BAP1 is also observed during adipogenesis, whereby UBE2O promotes differentiation and cytoplasmic localization of BAP1."

reach
"Of note, overexpression of UBE2O, but not its catalytic dead form, also promoted cytoplasmic localization of endogenous BAP1."
UBE2O binds BAP1.
5 | 1 1
5 | 1 1

No evidence text available

No evidence text available

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sparser
"Since BAP1 5K/R mutant still interact with UBE2O and is ubiquitinated on other sites, we also replaced the NLS region of BAP1, containing the UBE2O-interacting motif, with T-large antigen NLS which pr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"For example, UBE2O binds to and multi-monoubiquitinates BAP1 to induce its cytoplasmic localization and promotes adipocyte differentiation [XREF_BIBR]."
FOXK2 affects BAP1
11 | 6 15
11 | 6 14

No evidence text available

No evidence text available

No evidence text available

No evidence text available

sparser
"Furthermore, treatment of cells with the CDK inhibitor alsterpaullone had little effect on FOXK2BAP1 interactions (Supplementary Figure S2C)."

sparser
"It therefore appears likely that the R58A mutation does more than affect phospho-dependent interactions but underscores the importance of this domain in mediating interactions between FOXK2 and BAP1."

sparser
"Functional interactions between FOXK2 and BAP1 in target locus regulation."

No evidence text available

No evidence text available

sparser
"We also tested whether there was a reciprocal effect on FOXK2 binding upon BAP1 depletion but little effect was seen on the ChIP signal for FOXK2 following reductions in BAP1 expression levels by siRNA treatment (Figure xref ; Supplementary Figure S6B)."
FOXK2 binds PGR and BAP1. 1 / 1
| 1

sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
BAP1 affects cell death
| 1 29 1
BAP1 activates cell death.
| 1 21
| 1 21

reach
"Reduced BAP1 levels impair DNA repair as well as different forms of cell death and induce metabolic alterations that together favor cancer development and growth."

reach
"Although this remains to be investigated, these results, suggest that the majority of BAP1 partners regulate SLC7A11 expression and possibility impact ferroptosis.Because ferroptosis and apoptosis are two distinct programs in BAP1-mediated cell death, it will be worth to investigate whether these processes act in concert or independently during cancer development."

reach
"BAP1 induces cell death via interaction with 14-3-3 in neuroblastoma."

eidos
"- If BAP1 triggers cell death as discussed by the authors , could the authors be selecting for cells with reduced BAP1 knockdown in Fig. S2B ?"

reach
"Two different strategies were applied to analyze the mechanism of BAP1 inducing cell death in neuroblastoma."

reach
"Conversely, silencing BAP1 expression reduced the early stage apoptotic cell population to 2.71%, significantly lower than that of the control (5.25%, p < 0.05), suggesting that BAP1 promotes tumor cell death by apoptosis and necrosis."

reach
"Processes other than apoptosis could be also involved in the cell death mechanism mediated by BaP1 on BAEC and HeLa cell lines [[XREF_BIBR]]."

reach
"24 For example, BAP1 has been shown to enhance progression through the G1-S checkpoint and subsequently induce cell death by a process with similarities to both apoptosis and necrosis."

reach
"In contrast, at higher concentrations, BaP1 stains lipid droplets and induces a regulated cell death process mediated by vacuolar membrane permeabilization."

reach
"For example, BAP1 can enhance progression through the G1-S checkpoint and subsequently induce cell death by a process with similarities to both apoptosis and necrosis [XREF_BIBR]."
BAP1 inhibits cell death.
| 8 1
| 8 1

reach
"Changes in mitochondrial permeability are required for the activation of the apoptotic process and the loss of BAP1 protects the cells from caspase-induced cell death as a consequence of IP3R3 level reduction and Ca2 + signalling decrease [45]."

reach
"The powerful activity of heterozygous germline BAP1 mutations to facilitate asbestos induced transformation and cause mesothelioma has been linked to the alterations in the mechanisms that regulate DNA repair and cell death caused by decreased BAP1 protein levels."

reach
"BAP1 depletion, however, triggers cell death in many cell types, which could seem paradoxical considering its tumor suppressor function."

reach
"Thus, BAP1 might suppress tumorigenesis by facilitating tumor cell death by activation of both these pathways."

reach
"XREF_BIBR reported that BAP1 overexpression in the BAP1-null NCI-H226 human non-small-cell lung cancer cell line suppressed growth in DUB activity- and nuclear localization dependent manners by accelerating the cell cycle G1/S transition and inducing cell death."

reach
"Here we show that BAP1 inhibits cell death induced by unresolved metabolic stress."

reach
"Arabidopsis C2 domain proteins, BAP1, and its homologue BAP2, negatively regulate biotic and abiotic cell death."

sparser
"Here we show that BAP1 inhibits cell death induced by unresolved metabolic stress."

reach
"The group of flg22 induced MPK4 dependent genes encode important regulators of plant defence such as the cell death inhibitor BAP1 [XREF_BIBR], the calcium dependent protein kinase CPK5 [XREF_BIBR], the exocyst complex subunit EXO70B2 [XREF_BIBR], the cyclic nucleotide gated ion channel CNGC11 [XREF_BIBR] or the BAK1 like receptor kinase BKK1 and SERK4 [XREF_BIBR]."
BARD1 affects BAP1
| 1 7 19
| 1 6 11

reach
"BAP1 interacts with BARD1 (182aeuro " 365), HCF-1 (365aeuro " 385), BRCA1 (596aeuro " 721) and YY1 (642- 686).10,88 Numbers refer to amino acid positions."

reach
"BAP1 binds and deubiquitylates BARD1, thus modulating the E3 ligase activity of BRCA1-BARD1 28."

sparser
"The loss of the ring finger domain abrogates the binding of BARD1 and BAP1, which are important regulators of E3 ubiquitin ligase activity of BRCA1."

sparser
"Since BARD1 directly interacts with BAP1, the major impact of mutations in BARD1 (E182G, G185R) as compared to BRCA1 (E602D) can be seen in the structural analysis."

sparser
"The RING finger domain has been shown to mediate interactions with both BARD1 (see below) and a ubiquitin C-terminal hydrolase BAP1 [18] ."

sparser
"BAP1 binds and deubiquitylates BARD1, thus modulating the E3 ligase activity of BRCA1–BARD1 xref ."

sparser
"UCH is involved in the de-ubiquitin activity of BAP1 [ xref ], and BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1-BARD1 complex."

reach
"According to this hypothesis, Nishikawa et al showed that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of the BRCA1 and BARD1 complex, and that BAP1 and BRCA1 and BARD1 mutually regulate ubiquitination in the cell cycle and in the DNA damage response pathway."

sparser
"Domains comprised by residues 182-365 of BAP1 interact with the RING finger domain of BARD1, and surface plasmon resonance spectroscopy (BIAcore) analyses showed that BAP1 interferes with the BRCA1/BARD1 association."

reach
"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1 and BARD1."
BRCA1 binds BAP1 and BARD1. 6 / 6
| 1 5

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear and needs to be studied in more detail."

sparser
"BAP1 interacts with BARD1 and BRCA1 and inhibits the E3 ligase activity of BRCA1/BARD1."

sparser
"The ring domains of BRCA1 (residues 1-109) are associated with BARD1 [ xref ] and BAP1 [ xref ]."

sparser
"BAP1 and BRCA1-BARD1 complex coordinately regulate ubiquitination during the DNA damage response and the cell cycle [ xref , xref ]."

reach
"Until recently, the combination of pemetrexed and cisplatin was the only approved first-line therapy, yielding a median overall survival (OS) of 12.1 months.2 Nivolumab plus ipilimumab was approved recently as initial treatment for malignant pleural mesothelioma (MPM) on the basis of a phase 3 clinical trial that resulted in a median OS of 18.1 months.3 4 There are no approved therapies for patients with tumor relapse after first-line therapies.Risk factors in developing mesothelioma include exposure to asbestos5 and other carcinogenic fibers such as erionite,6 and previous radiation therapy.7 8 Studies of familial clusters of mesothelioma have revealed genetic predisposition to develop MM.9 10 Recent work has identified germline mutations in BAP1 that can predispose to mesothelioma11 12 and other cancers such as uveal and cutaneous melanoma, basal cell carcinoma, meningioma, and renal cell cancers.13 14 We previously identified deleterious germline mutations in 12% of 241 consecutive patients with MM who enrolled on a prospective natural history study, with BAP1 being the most frequent germline mutation.15 BAP1 was also recurrently inactivated at the somatic level, suggesting BAP1 variants undergoing positive selection in the context of the classic “two-hit” model.BAP1, a nuclear deubiquitylase, was initially discovered as an interaction partner of the tumor suppressor BRCA1.16 Subsequent studies revealed that BAP1 binds to BARD1, which, in turn, binds to BRCA1, forming the BRCA1-BARD1-BAP1 complex that is crucial for the homologous recombination (HR)–mediated repair of DNA double-strand breaks (DSBs).17 PARP enzymes play a major role in DNA single-strand break repair and base excision repair pathways."

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear."

sparser
"They found that BAP1 did not appear to be involved in the deubiquitination of the BRCA1/BARD1 complex,29 and BAP1-mediated growth suppression in vitro was shown to be independent of wild-type BRCA1.4 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"According to this hypothesis, Nishikawa et al showed that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of the BRCA1/BARD1 complex, and that BAP1 and BRCA1/BARD1 mutually regulate ubiquitination in the cell cycle and in the DNA damage response pathway. xref According to the present state of knowledge, it is reasonable to speculate that BAP1 may exert a regulatory role in the DNA damage response."

sparser
"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1/BARD1."
YUH1 affects BAP1
| 26
| 24

sparser
"These data suggest that the AB box binds the ULD domain first, and this complex then interacts with the BAP1-UCH domain to stimulate enzyme activity."

sparser
"Of note, the BAP1-UCH loop is larger than the ones in other UCH proteins, with a high conservation of both these loop amino acids and of multiple amino acids structurally near this loop ( xref , xref , xref ), implying that larger substrates may be available to BAP1’s catalytic cleavage site than for other UCH domains, yielding a BAP1-specific recruitment of proteins/domains such as ASX and ULD for enzyme regulation ( xref )."

sparser
"Several mutations significantly destabilize BAP1-UCH and increase its aggregation propensity."

sparser
"Homology of the BAP1-UCH and other UCH-like proteins infers that this BAP1 motif functions in either ubiquitin-mediated, proteasomal degradation or some other ubiquitin-facilitated regulatory pathways that are involved in BRCA1 function, cellular proliferation, differentiation, and/or homeostatic processes ( xref , xref , xref )."

sparser
"Loss of the DUB activity due to catalytic mutations within the ubiquitin C-terminal hydrolase (UCH) domain of BAP1 (BAP1-UCH) directly contributes to oncogenesis."

sparser
"Our findings gave a comprehensive and multiscale account of the molecular basis of how these non-catalytic mutations within BAP1-UCH may be implicated in oncogenesis."

sparser
"Nevertheless, it is non-trivial to rationalize how the other high-frequency but non-catalytic mutations within the BAP1-UCH lead to malignancies."

sparser
"Here we used multiplex spectroscopic, thermodynamic and biophysical analyses to investigate the impacts of eleven high-occurrence mutations within BAP1-UCH on the structure, folding and function."

sparser
"Here, we demonstrate that the ubiquitin C-terminal hydrolase (UCH) domain of BAP1 (BAP1-UCH) is highly sensitive to moderate oxidative stress."

sparser
"Therefore, we initiated biochemical and biophysical analyses of the BAP1-UCH, BAP1-ULD, ASXL2-AB domains and protein complex."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
SETD2 affects BAP1
| 1 24
| 1 9

sparser
"Our results showed that CGN expression was positively associated with tumor suppressors BAP1 ( xref ) and SETD2 ( xref )."

sparser
"Mutations in other genes like SETD2 and BAP1 which have been associated with inferior clinical outcomes in other studies were not found to be statistically significant in our analysis after adjustment."

sparser
"BAP1 and SETD2 mutations are associated with worse cancer specific survival and likely take place during the disease progression phase."

sparser
"BAP1 and SETD2 mutations (6–12%) are associated with worse CSS, suggesting their roles in disease progression."

reach
"The association between VHL, PBRM1, BAP1, SETD2 AND KDM5C mutation status and overall survival was evaluated in 167 patients with metastatic ccRCC who underwent genomic sequencing of their primary tumor."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."

sparser
"Here, we focus on the impact of individual mutations of PBRM1 , BAP1 , and SETD2 on cancer specific survival in an expanded version of our original cohort (3 additional patients and several more months of followup and events) and further demonstrate the association of BAP1 and SETD2 mutations with worse cancer specific survival (the TCGA cohort n=421), providing a molecular link between gene mutations and cancer specific outcomes in ccRCC."

sparser
"Similarly, a study of 609 patients with ccRCC from two distinct cohorts revealed that mutations of 3p21 epigenetic regulators BAP1 and SETD2 were associated with worse cancer-specific survival (CSS), suggesting that they play key roles in disease progression[ xref ]."

sparser
"Furthermore, PBRM1 mutations have been found to confer a slight increase of death risk, while SETD2 or BAP1 mutations were associated with serious death risks in ccRCC patients [ xref – xref ]."

sparser
"Across clear cell- and papillary-associated RCC subtypes in TCGA cohort, worse survival was associated with SETD2 or BAP1 mutation, with their related gene transcriptional signatures involving greater numbers of RCC cases and also being predictive of worse outcome ( xref , xref )."
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
SETD2 binds PBRM1 and BAP1. 3 / 3
| 3

sparser
"The mutations in the chromatin regulators PBRM1 , BAP1 , and SETD2 were differentially associated with altered expression patterns of large numbers of genes when compared to samples bearing a background of VHL mutation ( xref , xref )."

sparser
"In fact this association with chromosome 3p loss has recently been strengthened by several publications demonstrating that 4 out of five ( VHL , PBRM1 , SETD2 and BAP1 ) of the most frequently mutated genes associated with CCRCC are on chromosome 3p xref – xref and that the mutation of either SETD2 , BAP1 or PBRM1 associates with poorer prognosis or progression in those patients xref , xref , xref ."

sparser
"On univariable analysis SETD2 (HR: 3.30), BAP1 (HR: 2.44) and PBRM1 (HR: 1.78) were significantly associated with a higher risk of metastases."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
SETD2 binds MTOR and BAP1. 2 / 2
| 2

sparser
"By comparing tumours which cluster with the cell lines based on CNAs with tumours that cluster away from the cell lines, we found that the tumours likely to be best represented by the cell lines carry hallmarks of aggressive disease, such as higher stage, higher grade, greater extent of CNAs, and more frequent mutations in genes such as SETD2, BAP1 and MTOR , which have been associated with more aggressive disease and poorer outcomes."

sparser
"These tumours also display a higher extent of copy number aberrations (mean fraction genome altered: 19% versus 12%), and more frequent mutations in genes such as BAP1 (12.3% versus 5.4%), SETD2 (12.7% versus 8.1%) and MTOR (6% versus 4.7%), which are associated with more aggressive disease (though only BAP1 has a statistically significant difference— P value 0.025, Fisher's exact test)."
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
KMT2C affects BAP1
| 4 21
| 4 19

sparser
"More recently, PHD domain mutations in KMT2C were studied in human cells, revealing that these disrupt the interaction between KMT2C and the tumor suppressor BAP1."

sparser
"In fact, the crystal structure (PDB: 2YSM) of MLL3-PHD2–3 reveals that those mutations that are important for MLL3-BAP1 complex binding, are closely associated with the zinc atoms, which help maintain the structure of PHD fingers ( xref )."

sparser
"To investigate if the endogenous MLL3 also interacts with BAP1, we immunoprecipitated MLL3, MLL4, SET1A, and SET1B components of the COMPASS family from HEK293T cells using antibodies specific to each methyltransferase ( xref ; xref )."

sparser
"For instance, the BAP1 H2A deubiquitinase recruits H3K4 monomethylase MLL3 to monomethylate gene enhancers, while disruption of the interaction between BAP1 and MLL3 contributes to the pathogenesis of multiple cancers [ xref ]."

sparser
"Using a human breast cancer cell line, CAL51, we confirmed that these point mutations strongly diminish the interaction between BAP1 and MLL3-NTD ( xref )."

reach
"Recently identified missense mutations within the PHD fingers 1-3 have been shown to disrupt the interaction between KMT2C and BAP1 leading to reduced recruitment of KMT2C to gene enhancers 1."

sparser
"Recently identified missense mutations within the PHD fingers 1–3 have been shown to disrupt the interaction between KMT2C and BAP1 leading to reduced recruitment of KMT2C to gene enhancers xref ."

sparser
"In addition, a mutation hotspot within the PHD cluster of the KMT2C gene was described, the mutations of which disrupted the interaction of KMT2C with the BAP1 (BRCA1 associated protein-1) tumor suppressor, resulting in poor patient survival in many types of cancer (liver, lung, bladder, and breast)."

sparser
"Cancer-associated MLL3 PHD mutations disrupt the interaction between MLL3 and BAP1 and correlate with poor patient survival."

sparser
"Notably, BAP1 selectively associates with MLL3 but not with MLL4 indicating a bifurcation in the function of these complexes."
KDM6A binds KMT2C and BAP1. 2 / 2
| 2

sparser
"We found that cancer hotspot mutations in the MLL3 amino terminal PHD repeats disrupt its association with the BAP1 complex and result in reduced MLL3 and UTX recruitment to enhancers misregulating the epigenetic chromatin state at these loci."

sparser
"Mutations within the MLL3 PHD domains abolish the interaction between the BAP1 tumor suppressor complex and MLL3-UTX, which leads to attenuation of enhancer activity."
BAP1 affects SETD2
| 1 24
| 1 9

sparser
"Our results showed that CGN expression was positively associated with tumor suppressors BAP1 ( xref ) and SETD2 ( xref )."

sparser
"Mutations in other genes like SETD2 and BAP1 which have been associated with inferior clinical outcomes in other studies were not found to be statistically significant in our analysis after adjustment."

sparser
"BAP1 and SETD2 mutations are associated with worse cancer specific survival and likely take place during the disease progression phase."

sparser
"BAP1 and SETD2 mutations (6–12%) are associated with worse CSS, suggesting their roles in disease progression."

reach
"The association between VHL, PBRM1, BAP1, SETD2 AND KDM5C mutation status and overall survival was evaluated in 167 patients with metastatic ccRCC who underwent genomic sequencing of their primary tumor."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."

sparser
"Here, we focus on the impact of individual mutations of PBRM1 , BAP1 , and SETD2 on cancer specific survival in an expanded version of our original cohort (3 additional patients and several more months of followup and events) and further demonstrate the association of BAP1 and SETD2 mutations with worse cancer specific survival (the TCGA cohort n=421), providing a molecular link between gene mutations and cancer specific outcomes in ccRCC."

sparser
"Similarly, a study of 609 patients with ccRCC from two distinct cohorts revealed that mutations of 3p21 epigenetic regulators BAP1 and SETD2 were associated with worse cancer-specific survival (CSS), suggesting that they play key roles in disease progression[ xref ]."

sparser
"Furthermore, PBRM1 mutations have been found to confer a slight increase of death risk, while SETD2 or BAP1 mutations were associated with serious death risks in ccRCC patients [ xref – xref ]."

sparser
"Across clear cell- and papillary-associated RCC subtypes in TCGA cohort, worse survival was associated with SETD2 or BAP1 mutation, with their related gene transcriptional signatures involving greater numbers of RCC cases and also being predictive of worse outcome ( xref , xref )."
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
SETD2 binds PBRM1 and BAP1. 3 / 3
| 3

sparser
"The mutations in the chromatin regulators PBRM1 , BAP1 , and SETD2 were differentially associated with altered expression patterns of large numbers of genes when compared to samples bearing a background of VHL mutation ( xref , xref )."

sparser
"In fact this association with chromosome 3p loss has recently been strengthened by several publications demonstrating that 4 out of five ( VHL , PBRM1 , SETD2 and BAP1 ) of the most frequently mutated genes associated with CCRCC are on chromosome 3p xref – xref and that the mutation of either SETD2 , BAP1 or PBRM1 associates with poorer prognosis or progression in those patients xref , xref , xref ."

sparser
"On univariable analysis SETD2 (HR: 3.30), BAP1 (HR: 2.44) and PBRM1 (HR: 1.78) were significantly associated with a higher risk of metastases."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
SETD2 binds MTOR and BAP1. 2 / 2
| 2

sparser
"By comparing tumours which cluster with the cell lines based on CNAs with tumours that cluster away from the cell lines, we found that the tumours likely to be best represented by the cell lines carry hallmarks of aggressive disease, such as higher stage, higher grade, greater extent of CNAs, and more frequent mutations in genes such as SETD2, BAP1 and MTOR , which have been associated with more aggressive disease and poorer outcomes."

sparser
"These tumours also display a higher extent of copy number aberrations (mean fraction genome altered: 19% versus 12%), and more frequent mutations in genes such as BAP1 (12.3% versus 5.4%), SETD2 (12.7% versus 8.1%) and MTOR (6% versus 4.7%), which are associated with more aggressive disease (though only BAP1 has a statistically significant difference— P value 0.025, Fisher's exact test)."
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
BAP1 affects KMT2C
| 4 21
| 4 19

sparser
"More recently, PHD domain mutations in KMT2C were studied in human cells, revealing that these disrupt the interaction between KMT2C and the tumor suppressor BAP1."

sparser
"In fact, the crystal structure (PDB: 2YSM) of MLL3-PHD2–3 reveals that those mutations that are important for MLL3-BAP1 complex binding, are closely associated with the zinc atoms, which help maintain the structure of PHD fingers ( xref )."

sparser
"To investigate if the endogenous MLL3 also interacts with BAP1, we immunoprecipitated MLL3, MLL4, SET1A, and SET1B components of the COMPASS family from HEK293T cells using antibodies specific to each methyltransferase ( xref ; xref )."

sparser
"For instance, the BAP1 H2A deubiquitinase recruits H3K4 monomethylase MLL3 to monomethylate gene enhancers, while disruption of the interaction between BAP1 and MLL3 contributes to the pathogenesis of multiple cancers [ xref ]."

sparser
"Using a human breast cancer cell line, CAL51, we confirmed that these point mutations strongly diminish the interaction between BAP1 and MLL3-NTD ( xref )."

reach
"Recently identified missense mutations within the PHD fingers 1-3 have been shown to disrupt the interaction between KMT2C and BAP1 leading to reduced recruitment of KMT2C to gene enhancers 1."

sparser
"Recently identified missense mutations within the PHD fingers 1–3 have been shown to disrupt the interaction between KMT2C and BAP1 leading to reduced recruitment of KMT2C to gene enhancers xref ."

sparser
"In addition, a mutation hotspot within the PHD cluster of the KMT2C gene was described, the mutations of which disrupted the interaction of KMT2C with the BAP1 (BRCA1 associated protein-1) tumor suppressor, resulting in poor patient survival in many types of cancer (liver, lung, bladder, and breast)."

sparser
"Cancer-associated MLL3 PHD mutations disrupt the interaction between MLL3 and BAP1 and correlate with poor patient survival."

sparser
"Notably, BAP1 selectively associates with MLL3 but not with MLL4 indicating a bifurcation in the function of these complexes."
KDM6A binds KMT2C and BAP1. 2 / 2
| 2

sparser
"We found that cancer hotspot mutations in the MLL3 amino terminal PHD repeats disrupt its association with the BAP1 complex and result in reduced MLL3 and UTX recruitment to enhancers misregulating the epigenetic chromatin state at these loci."

sparser
"Mutations within the MLL3 PHD domains abolish the interaction between the BAP1 tumor suppressor complex and MLL3-UTX, which leads to attenuation of enhancer activity."
| 3 21
| 3 12

reach
"Early changes resulting in gain of chromosome 8q may activate macrophage infiltration, while sequential loss of BAP1 expression seems to drive T cell infiltration in UM."

eidos
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation , cell cycle progression , and invasion via inhibiting ERK1 / 2 and JNK / c-Jun signaling pathways ."

reach
"Importantly, loss of BAP1 decreased the formation of stress fibers and membrane protrusions and induced migration and invasion defects."

reach
"Likewise, cell proliferation, G1-phase to S-phase cell cycle progression, and invasion of HCCC9810 cells were significantly inhibited by transfection of wild-type BAP1 but not inactive mutant BAP1C91A (Fig. 4c, d)."

reach
"Functionally, BAP1 promotes apoptosis and necrosis, and down-regulates the migration and invasion abilities of LAC cells."

reach
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways."

reach
"In functional assays, BAP1 was found to inhibit the migration and invasion of LAC cells, and promoted their apoptosis and necrosis."

reach
"Moreover, both scratching assay and Matrigel invasion assay indicated that overexpression of BAP1 in HCCC9810 cells significantly reduced cell migratory and invasion capacities, while the motility and invasion potential of RBE-shBAP1 cells were evidently enhanced compared to RBE-Mock cells (Fig. 2d, e)."

reach
"These results indicated that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways.To further validate these findings, we used inhibitors of the ERK1/2 pathway (U0126) and JNK/c-Jun pathway (SP600125) in RBE-shBAP1 cells."

reach
"Wild-type BAP1 but not mutant BAP1 significantly inhibited HCC cell proliferation, invasion, epithelial-mesenchymal transition (EMT) in vitro, and tumor progression and metastasis in vivo."
| 9

reach
"Consistently, silence of BAP1 or KLF5 expression significantly decreased the invasion of HCC1937 cells in transwell matrigel invasion assays (XREF_FIG)."

reach
"We also report a positive correlation between BAP1 and patient outcome and provide evidence that BAP1 overexpression promotes migration and invasion of adenocarcinoma."

reach
"BRCA1 associated protein 1 deficiency in lung adenocarcinoma predicts poor outcome and increased tumor invasion."

reach
"BAP1 may inhibit the progression of LAC by attenuating tumor invasiveness, a function that was supported by the findings of the Transwell assay."

reach
"BAP1 and KLF5 promote breast cancer migration, invasion and metastasis may not through p27; however, KLF5 is a transcription factor regulating numerous downstream target genes, such as FGF-BP and mPGES1."

reach
"Germline or somatic mutations and deletions of BAP1 have been identified in various tumor types, and downregulation or inactivation of BAP1 could accelerate tumor onset, invasion, recurrence, and metastases ."

reach
"Taken together, these data revealed that downregulation of BAP1 promotes proliferation, G1 to S cell cycle progression, and invasion of ICC in vitro."

reach
"Downregulation of BAP1 promotes proliferation, cell cycle progression, and invasion of ICC in vitro."
| 1

reach
"BAP1 mutation increased the infiltration degree of Treg cells."
| 18 5
BAP1 activates Multiple Myeloma.
| 11

reach
"Importantly, however, our data suggest that dysregulation of the Rb path way may be required for Bap1 driven MM pathogenesis, at least in an experimental model."

reach
"While homozygous CKO of Bap1, Cdkn2a, or Nf2 alone gave rise to few or no MMs, inactivation of Bap1 cooperated with loss of either Nf2 or Cdkn2a to drive development of MM in ~ 20% of double-CKO mice, and a high incidence (22/26, 85%) of MMs was observed in Bap1; Nf2; Cdkn2a (triple)-CKO mice."

reach
"Rather, the presence of a wild type allele in most studied cases, suggests that BAP1 haplo-insufficiency may lead to peritoneal MM."

reach
"Recently, Bap1 +/- knockout mice were found to have increased susceptibility to asbestos induced MM compared to wild type litter mates (Xu et al., 2014)."

reach
"There was no association between total SNV and survival; ever versus never smokers; or patients with epithelioid versus non epithelioid histologies.The three archetypical somatic drivers of MM - BAP1,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"BAP1 stabilizes BRCA1 protein and restores cell survival rates of MM cells with BAP1 deletion after IR."

reach
"While most human MMs exhibit somatic alterations of BAP1, NF2 and/or CDKN2A, currently it is not known if inactivation of BAP1 cooperates with loss of NF2 and/or CDKN2A to drive a more aggressive MM phenotype."
Mutated BAP1 activates Multiple Myeloma. 4 / 4
| 4

reach
"We recently reported in vivo genetic evidence that germline heterozygous mutation of Bap1 accelerates development of asbestos induced MM."

reach
"BAP1 is the most promising driver gene in MM pathogenesis, because more than 60% of MM biopsies contain BAP1 mutations and inactivation [XREF_BIBR, XREF_BIBR] and germline BAP1 mutations cause a cancer syndrome with high incidence of MM [XREF_BIBR, XREF_BIBR]."

reach
"A possible explanation is that germline BAP1 mutations alone can cause MM and many other tumor types."

reach
"We are investigating how germline BAP1 mutations on one hand promote MM development, while on the other hand they are associated to reduce disease aggressiveness."
| 7

reach
"Loss of BAP1 is frequently observed in MM and has been proven to increase the risk of MM [XREF_BIBR - XREF_BIBR]."

reach
"Transduction of the WT BAP1 vector inhibited cell proliferation of the Y-MESO-25 MM cell line with BAP1 deletion by approximately 50% compared to the cells with control vector (GFP)."

reach
"Our results suggested that, whereas WT BAP1 suppresses MM cell proliferation and restores cell survival after IR damage, some mutant BAP1 may also moderately retain these functions."

reach
"BAP1 suppresses MM cell proliferation."

reach
"In effusions with mesotheliomatous cells or atypical mesothelial cells of uncertain significance, negative BAP1 IHC strongly supports a diagnosis of MM."

reach
"Bap1 deletion alone does not cause MM but dramatically accelerates MM development when combined with Nf2 and Cdkn2ab (hereafter BNC) disruption."

reach
"With the colony formation assay, we also found that WT BAP1 suppressed the anchorage independent cell growth of the MM cells with BAP1 deletion, whereas mutant BAP1 showed little or no reduced effect."
| 5

sparser
"BAP1 knockdown in MM cell lines has been paradoxically associated to a decreased proliferation, with an accumulation of cells in the S phase of the cell cycle xref , suggesting that BAP1 loss might promote tumorigenesis inducing a delayed, but more permissive, G1/S checkpoint xref ."

sparser
"BRCA1-associated protein-1 ( BAP1 ) mutations is known to be related to a high rate of MM and MM associated with germline BAP1 mutations has a better prognosis of overall 7-fold increased long-term s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The association of germline BAP1 mutations with familial MM has been confirmed in a series of recent reports ( xref - xref )."

sparser
"Here, we tested the hypothesis that MM associated with germline BAP1 mutations has a better prognosis compared with sporadic MM."

sparser
"The demonstration that BAP1 mutations are strongly associated with MM has provided a real breakthrough in the study of genetic predisposition in MM, that may explain why only a fraction of asbestos-exposed individuals go on to develop MM."
| 22
| 12

reach
"Both ASXL1 and BAP1 were downregulated during RA induced P19 cell differentiation with concomitant increase of ubiquitinated H2B, leading to activation of Hox genes."

reach
"BAP1 promotes progenitor cell differentiation into melanocytes by increasing MITF expression and BAP1 loss may promote metastasis in UM by remodeling the epigenome to resemble that of stem-like migratory neural crest cells."

reach
"Downregulation of ASXL1 and BAP1 Promotes Hox Gene Exp (A) Downregulation of ASXL1 and BAP1 during RA induced differentiation."

reach
"As a deubiquitinating enzyme, BAP1 contributes to gene transcription, cell differentiation, DNA damage repair, apoptosis, and cell metabolism in tumor inhibition."

reach
"Concomitant Bap1 and Ezh2 loss reduced myeloid progenitor expansion, reduced the proportion of Mac1 + Gr1 + myeloid cells, restored erythroid differentiation (CD71 + Ter119 +) and decreased proliferation of Bap1 and Ezh2 deficient progenitors (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"6 In line with these findings, several reports have indicated that truncated ASXL1 enhances BAP1 complex activity, thereby promoting depletion of the H2AK119Ub mark and aberrant myeloid differentiation."

reach
"Interestingly, ASXL1-MT and BAP1 promotes monocyte differentiation, while it inhibits terminal differentiation to macrophage, which may account for the frequent (40-50%) detection of ASXL1 mutations in CMML patients."

reach
"In the monocyte and macrophage differentiation assay, coexpression of ASXL1-MT and BAP1 substantially promoted differentiation toward CD115 + monocytes while inhibiting their terminal differentiation to CD115 + F4/80 + macrophages."

reach
"BAP1 depletion caused a reduction in mRNA levels of neural crest migration genes (ROBO1), melanocyte differentiation genes (CTNNB1, EDNRB and SOX10) and other genes that are down-regulated in class 2 tumors (LMCD1 and LTA4H)."

reach
"Paradoxically, recent findings have confirmed that BAP1 also promotes growth and differentiation even in uveal melanomas where loss of BAP1 expression is a common event."
BAP1 bound to ASXL1-MT activates cell differentiation. 2 / 2
| 2

reach
"Thus, Irf8 is also a downstream target of the ASXL1-MT and BAP1 complex, which is likely to underlie the enhanced monocytic differentiation of HSPCs coexpressing ASXL1-MT and BAP1."

reach
"This hyperactive ASXL1-MT and BAP1 complex promotes aberrant myeloid differentiation of haematopoietic progenitor cells and accelerates RUNX1-ETO-driven leukaemogenesis."
| 10

reach
"Consistent with those results, treatment of BAP1 deficient uveal melanoma cells with an HDAC inhibitor restored the expression of the melanocyte differentiation markers, which were down-regulated by BAP1 depletion, in a dose dependent manner."

reach
"The recent finding that BAP1 loss leads to defective differentiation and an arrested primitive phenotype in vertebrate development and uveal melanoma suggests that BAP1 loss can promote tumor progression by inducing cell dedifferentiation and stem like behavior."

reach
"However, as previously reported, depletion of BAP1 in UM cells induced loss of melanocytic differentiation together with acquisition of a gene expression profile seen in advanced metastasizing tumors."

reach
"Studies suggest that down regulated expression of BAP1 led to impaired differentiation in UM cells."

reach
"ASXL1-MT alone modestly, and coexpression of ASXL1-MT and BAP1 dramatically blocked G-CSF-induced granulocytic differentiation of 32Dcl3."

reach
"In functional studies, depletion of BAP1 protein in the 92.1 UM cell line using siRNAs led to a change in cell morphology from spindle to a more epithelioid phenotype, loss of melanocytic differentiation and transition from a ' class 1 ' to a ' class 2 ' gene expression profile."

reach
"Depletion of BAP1 in cultured class 1 UM cells induced a loss of melanocytic differentiation and acquisition of a class 2 gene expression profile, suggesting that the loss of BAP1 may be mechanistically linked to metastasis."

reach
"ASXL1-MT and BAP1 impairs multilineage differentiation of HSPCs."
BAP1 bound to ASXL1-MT inhibits cell differentiation. 2 / 2
| 2

reach
"The ASXL1-MT and BAP1 complex is not a simple growth-accelerator, but rather inhibits terminal differentiation of HSPCs toward granulocytes, macrophages, mast cells, and erythrocytes."

reach
"Together, these results indicate that the ASXL1-MT and BAP1 complex impairs terminal differentiation of HSPCs, except for differentiation toward monocytes."
BAP1 affects YY1
5 1 | 6 7
BAP1 binds YY1.
5 | 6 7
5 | 4 2

sparser
"Instead, association of Yin Yang 1 with BAP1 could be the remnant of an ancient association between Pleohomeotic and Calypso, which would have allowed for the recognition of Polycomb response elements by the Drosophila PR-DUB complex."

No evidence text available

reach
"may be important for the growth effects of HCF-1, which are partly mediated by modulation, at E2F responsive promoters, of progression of cells through the G1/S-phase boundary and transcription of gen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Moreover, HCF-1 interacts with the middle region of YY1 encompassing the glycine-lysine-rich domain and is essential for the formation of a ternary complex with YY1 and BAP1 in vivo."

No evidence text available

reach
"93 In addition, the transcriptional repressor protein YY1, which functions as a PcG protein in Drosophila, 99 also interacts with BAP1."

No evidence text available

No evidence text available

No evidence text available

reach
"BAP1 interacts with BARD1 (182aeuro " 365), HCF-1 (365aeuro " 385), BRCA1 (596aeuro " 721) and YY1 (642- 686).10,88 Numbers refer to amino acid positions."
YY1 binds HCFC1 and BAP1. 7 / 7
| 2 5

sparser
"BAP1 forms a ternary complex with HCF1 and YY1, which acts as transcriptional repressor of genes involved in cell proliferation ( xref )."

sparser
"31 Further, BAP1 forms a protein complex with the host cell factor 1 and Yin and Yang 1."

sparser
"3,10,12 BAP1 and HCF-1 can also form a complex with the transcription factor YY1, which recruits BAP1 and HCF-1 to various promoters to regulate gene expression."

reach
"Prominent among the positively enriched gene sets were those related to writers of histone H3K27Me3, including PRC2 and SUZ12 (XREF_FIG), as well as genes participating in cell proliferation and transcription regulation involving ubiquitin signaling mediated by the Bap1, HCF1, and YY1 complex, EED related stem cell pluripotency, cytokine signaling, and cell adhesion; 100 of the top pathways differentially affected in MMs from TKO versus DKO mice are presented in XREF_SUPPLEMENTARY."

reach
"Our RNA-seq analysis revealed that MMs from Bap1; Nf2; Cdkn2a CKO mice exhibit positively enriched gene sets consistent with cellular processes previously implicated in Bap1 function, such as genes mediated by the Bap1, HCF1, and YY1 complex, which play a role in cellular proliferation and transcription regulation involving ubiquitin signaling."

sparser
"Yu H, Mashtalir N, Daou S, et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression."

sparser
"Finally, BAP1 forms complexes with the transcription factors Yin Yang 1 (YY1) and host cell factor 1 (HCF-1), and controls cell-cycle progression at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters [ xref , xref ]."
BAP1 deubiquitinates YY1.
1 |
BAP1 deubiquitinates YY1. 1 / 1
1 |

"BAP1 also controls G1/S cell cycle progression by regulating BRCA-1 [111], Ying Yang 1 (YY-1), and host cell factor 1 (HCF-1)"
BAP1 affects ITPR3
| 2 14 4
BAP1 binds ITPR3.
| 3 4
| 2 4

sparser
"Then BAP1 binds and deubiquitinates IP3R3 in the ER, leading to Calcium release into the cytosol and mitochondria, and initiation of apoptotic pathways."

reach
"The data clearly indicated that BAP1 physically interacts with the type-3 inositol-1,4,5-trisphosphate-receptor (IP3R3), and BAP1 expression level is directly correlated with IP3R3 in wild-type and mutant fibroblasts."

sparser
"We confirmed the specificity of the BAP1 interaction with IP3R3 in HEK-293 cells expressing FLAG-BAP1 ( xref )."

sparser
"Recently, a novel BAP1 cytoplasmic function was introduced, showing that ER-localized BAP1 can bind and stabilize IP3R3 to modulate Ca2+ release resulting in apoptosis [ xref ]."

sparser
"We found that BAP1 binds the N-terminus domain of IP3R3 [IP3R3(NT)] ( xref )."

reach
"Overexpression of the N-terminus of the IP3R3 binds and sequesters BAP1 leading to decreased IP3R3 levels, an effect counter balanced by BAP1 co-overexpression (XREF_FIG)."
ITPR3 binds BAP1-C91S. 1 / 2
| 1

reach
"Instead, overexpression of the Myc tagged truncated BAP1 L-mutant [Myc-BAP1 (L)] (the L family mutant BAP1 which lacks the N-terminus domain 5) showed an almost complete loss of interaction with IP3R3 compared to Myc tagged WT BAP1 (Myc-BAP1) (XREF_FIG), while catalytic inactive BAP1 (C91S) XREF_BIBR, XREF_BIBR did bind IP3R3 (XREF_FIG)."
BAP1 increases the amount of ITPR3.
| 6
Modified BAP1 increases the amount of ITPR3. 3 / 3
| 3

reach
"Consequently, heterozygous loss of BAP1 leads to decreased IP3R3 levels and resistance to apoptotic stimuli, as demonstrated in human fibroblasts or mesothelial cells derived from mutant BAP1 +/- carriers and in mesothelioma cell lines 174."

reach
"Loss of BAP1 therefore leads to loss of ITPR3, which in turn reduces apoptotic mitochondrial calcium signals [XREF_BIBR]."

reach
"Reduced levels of BAP1 in BAP1 +/- carriers caused reduction of both IP3R3 levels and Ca 2+ flux, preventing BAP1 +/- cells that had accumulated DNA damage from executing apoptosis."
BAP1 increases the amount of ITPR3. 3 / 3
| 3

reach
"Remarkably, depletion of BAP1 or IP3R3 promoted crocidolite asbestos and TNF-alpha-induced transformation of primary mesothelial cells, and re-expression of IP3R3 in BAP1 depleted cells partially reverted the phenotype."

reach
"Transduction of BAP1 +/- fibroblasts with adenoviruses encoding BAP1, but not the catalytic inactive mutant BAP1 (C91S) XREF_BIBR, XREF_BIBR, rescued IP3R3 protein levels (XREF_FIG), mitochondrial Ca 2+ uptake (XREF_FIG, XREF_FIG, XREF_SUPPLEMENTARY) and resulted in enhanced apoptosis (XREF_FIG)."

reach
"Silencing of BAP1 in BAP1 WT fibroblasts consistently caused a reduction of IP3R3 protein levels (XREF_FIG), with concomitant reduction of mitochondrial Ca 2+ responses (XREF_FIG, and XREF_SUPPLEMENTARY), and protection from apoptosis (XREF_FIG)."
BAP1 deubiquitinates ITPR3.
| 3
BAP1 deubiquitinates ITPR3. 3 / 3
| 3

reach
"We hypothesized that BAP1 might deubiquitylate and stabilize IP3R3."

reach
"At the ER, BAP1-dependent deubiquitination of the type 3 inositol-1,4,5-trisphosphate receptor (IP3R3) is necessary to sustain the IP3R3 ER channel integrity (11,55)."

reach
"Along with maintenance of IP3R3 ER channel integrity, BAP1-dependent deubiquitination of IP3R3 may change the threshold for IP3R3 activation by Ca itself or IP3."
BAP1 activates ITPR3.
| 2 1
BAP1 activates ITPR3. 3 / 3
| 2 1

reach
"In the cytoplasm, BAP1 modulates the stability of the IP3R3 channel, which allows the flux of Ca + from the endoplasmic reticulum into the mitochondria, where Ca + is required for the Krebs cycle and, at higher doses, to execute apoptosis."

eidos
"Thus , loss of BAP1 leads to decreased ITPR3 expression , decreased mitochondrial Ca2 + and reduced sensitivity of cancer cells to apoptosis ."

eidos
"Loss of BAP1 therefore leads to loss of ITPR3 , which in turn reduces apoptotic mitochondrial calcium signals [ 6 ] ."
BAP1 decreases the amount of ITPR3.
| 1
BAP1 decreases the amount of ITPR3. 1 / 2
| 1

reach
"BAP1 deletion increased tumorigenesis by decreasing levels of IP3R3, Ca flux, and apoptosis upon stimulation by various stressors (55)."
YY1 affects BAP1
5 | 6 7
5 | 4 2

sparser
"Instead, association of Yin Yang 1 with BAP1 could be the remnant of an ancient association between Pleohomeotic and Calypso, which would have allowed for the recognition of Polycomb response elements by the Drosophila PR-DUB complex."

No evidence text available

reach
"may be important for the growth effects of HCF-1, which are partly mediated by modulation, at E2F responsive promoters, of progression of cells through the G1/S-phase boundary and transcription of gen[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Moreover, HCF-1 interacts with the middle region of YY1 encompassing the glycine-lysine-rich domain and is essential for the formation of a ternary complex with YY1 and BAP1 in vivo."

No evidence text available

reach
"93 In addition, the transcriptional repressor protein YY1, which functions as a PcG protein in Drosophila, 99 also interacts with BAP1."

No evidence text available

No evidence text available

No evidence text available

reach
"BAP1 interacts with BARD1 (182aeuro " 365), HCF-1 (365aeuro " 385), BRCA1 (596aeuro " 721) and YY1 (642- 686).10,88 Numbers refer to amino acid positions."
YY1 binds HCFC1 and BAP1. 7 / 7
| 2 5

sparser
"BAP1 forms a ternary complex with HCF1 and YY1, which acts as transcriptional repressor of genes involved in cell proliferation ( xref )."

sparser
"31 Further, BAP1 forms a protein complex with the host cell factor 1 and Yin and Yang 1."

sparser
"3,10,12 BAP1 and HCF-1 can also form a complex with the transcription factor YY1, which recruits BAP1 and HCF-1 to various promoters to regulate gene expression."

reach
"Prominent among the positively enriched gene sets were those related to writers of histone H3K27Me3, including PRC2 and SUZ12 (XREF_FIG), as well as genes participating in cell proliferation and transcription regulation involving ubiquitin signaling mediated by the Bap1, HCF1, and YY1 complex, EED related stem cell pluripotency, cytokine signaling, and cell adhesion; 100 of the top pathways differentially affected in MMs from TKO versus DKO mice are presented in XREF_SUPPLEMENTARY."

reach
"Our RNA-seq analysis revealed that MMs from Bap1; Nf2; Cdkn2a CKO mice exhibit positively enriched gene sets consistent with cellular processes previously implicated in Bap1 function, such as genes mediated by the Bap1, HCF1, and YY1 complex, which play a role in cellular proliferation and transcription regulation involving ubiquitin signaling."

sparser
"Yu H, Mashtalir N, Daou S, et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression."

sparser
"Finally, BAP1 forms complexes with the transcription factors Yin Yang 1 (YY1) and host cell factor 1 (HCF-1), and controls cell-cycle progression at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters [ xref , xref ]."
KLF5 affects BAP1
5 | 9 6
5 | 9 6

No evidence text available

No evidence text available

reach
"BAP1 interacts directly with KLF5 and stabilizes KLF5 via deubiquitination."

reach
"Because KLF5 interacted with BAP1 directly, we wondered whether KLF5 was in the BAP1 and HCF -1 complex."

sparser
"Another KLF5 DUB that stood out in siRNA screening was BAP1 (BRCA1-associated protein 1), which also directly binds KLF5 and stabilizes KLF5 through deubiquitination [ xref ]."

reach
"BAP1 interacts directly with KLF5."

sparser
"BAP1 interacts directly with KLF5 and stabilizes KLF5 via deubiquitination."

sparser
"The deubiquitinase (DUB) BAP1 had been reported to regulate KLF5 stability in breast cancer cells ( xref , xref ), so we hypothesize that LINC00152 mediates KLF5 depending on the binding of BAP1 and KLF5."

No evidence text available

sparser
"As shown in xref , LINC00152 knockdown inhibits BAP1 binding with KLF5."
BAP1 affects EZH2
| 3 15 2
BAP1 inhibits EZH2.
| 3 8
BAP1 inhibits EZH2. 10 / 11
| 3 8

reach
"BAP1 loss leads to reduced L3MBTL2 stability and increased EZH2 transcriptional output (XREF_FIG)."

eidos
"BAP1 loss also up-regulates the expression of EZH2 , a Polycomb Repressive Complex-2 ( PRC2 ) component involved in epigenetic silencing ( 59 ) and oncogenic pathways ( 60 ) , suggesting sensitivity of BAP1-deficient MPM tumors to EZH2 inhibition ."

eidos
"Indeed , we observed that loss of BAP1 results in elevated EZH2 expression and increased H3K27me3 levels , and knock-down of Ezh2 leads to decreased H3K27me3 levels in BNC cells ( Fig. 5 , B and C ; and Fig. S2 J ) ."

reach
"Overexpression of EZH2 is reported in a range of malignancies, and a link between BAP1 loss and overexpression of EZH2 suggests a role for the PRC2 pathway and H3K27 hypermethylation in mesothelioma development and progression (122)."

reach
"Loss of BAP1 leads to reduced L3MBTL2 stability and increased EZH2 transcriptional output in mesothelioma [XREF_BIBR]."

reach
"Concomitant Bap1 and Ezh2 loss reduced myeloid progenitor expansion, reduced the proportion of Mac1 + Gr1 + myeloid cells, restored erythroid differentiation (CD71 + Ter119 +) and decreased proliferation of Bap1 and Ezh2 deficient progenitors (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"Loss of BAP1 has been shown to be synthetically lethal with inhibition of EZH2 or PRC2."

reach
"To that end, a systematic literature review was performed of articles dealing with a loss of BRCA1-associated protein 1 (BAP1), methylthioadenosine (MTAP), 5-hydroxymethylcitosine (5-hmC), glucose transporter 1 (GLUT1), insulin like-growth factor II messenger RNA-binding protein 3 (IMP3), enhanced zeste homologue 2 (EZH2) staining, cyclin-dependent kinase inhibitor 2A (CDKN2A) homozygous deletion (HD) testing, soluble mesothelin, and microRNA quantification in cytological samples for the diagnosis of MPM versus reactive atypical mesothelial cells."

eidos
"BAP1 loss leads to increased expression of EZH2 ( 15 ) ."

reach
"As for the reported tumor suppressive mechanism, BAP1 can antagonize EZH2 and PRC2 or RING1B (RNF2)/PRC1 in a tissue specific manner."
BAP1 decreases the amount of EZH2.
| 3
Modified BAP1 decreases the amount of EZH2. 3 / 3
| 3

reach
"Re-expression of BAP1 in Bap1 KO bone marrow cells reduced Ezh2 mRNA expression to normal levels (XREF_SUPPLEMENTARY)."

reach
"This could represent a possible connection between BAP1 and expression of HLA in uveal melanoma, as study in mice showed that loss of BAP1 leads to increased levels of EZH2 (and PRC2) with repressed expression of its targets [XREF_BIBR], including thus CIITA, leading ultimately to less HLA class II expression."

reach
"Recurrent BAP1 mutations have also been observed in intrahepatic cholangiocarcinoma 25, and a recent study showed that loss of Bap1 in mice increases expression of H3K27me3 and Ezh2 while suppressing expression of PRC2 targets 26."
BAP1 binds EZH2.
| 1 2
| 1 2

sparser
"In these lung-iPSCs, the expression of EZH2 (the main component of PRC2 complex) is also very high, and the physical interactions of AXSL proteins with EZH2 and BAP1 is also reported [ xref , xref , xref ]."

sparser
"Survival analysis revealed that BAP1 loss, but not high EZH2 expression, was associated with a better prognosis."

reach
"We did not identify an interaction between BAP1 and EZH2 by co-immunoprecipitation (XREF_FIG)."
BAP1 activates EZH2.
| 3
BAP1 activates EZH2. 3 / 3
| 3

reach
"Frequent loss-of-function mutations in epigenetic factors, such as ARID1A, SMARCA4, SMARCB1, BAP1, and KDM6A, are likely to elicit the EZH2 and PRC2 addicted situation."

reach
"In line with the observations in myeloid cells, Bap1 deleted mouse MM cell proliferation is impaired upon shRNA mediated down-regulation of Ezh2."

reach
"99 In addition, RCC derived cell lines deficient in BAP1 overexpress EZH2, and are sensitive to EZH2 inhibitors in vitro."
BAP1 affects BAP1
| 17
BAP1 inhibits BAP1.
| 4
BAP1 inhibits BAP1. 4 / 6
| 4

reach
"In these haplo-insufficient cells (designated as HAP1 BAP1 KO), there was a complete loss of BAP1 compared with the parental HAP1 WT cells."

reach
"There were no chromosomal losses in either the Bap1 or Nf2 regions."

reach
"Overexpression of wild-type BAP1 reversed the sensitivity of BAP1 knockout cells to RITA, while overexpression of the mutant BAP1 (C91S) with inactivated ubiquitinase did not produce any rescue effect."

reach
"Tumors with both miR-31 and BAP1 overexpression exhibited significantly higher levels of BAP1 compared to tumors with miR-31 overexpression, suggesting that BAP1 overexpression could rescue the BAP1 suppression caused by miR-31."
BAP1 activates BAP1.
| 6
BAP1 activates BAP1. 6 / 6
| 6

reach
"The BAP1 cancer syndrome (Mendelian Inheritance in Man Tumor Predisposition Syndrome # 614327) is caused by heterozygous germline mutation in the BRCA1 associated protein-1 gene (BAP1) located at chromosome 3p21.1."

reach
"Identified in 2011, the inherited cancer predisposition syndrome caused by germline mutations in the tumor suppressor gene BAP1 (BRCA1 Associated Protein 1) has shed light for the first time on monoge[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Heterozygous loss of BAP1 would reduce overall levels of BAP1 protein, disfavouring bidentate complex assembly and decreasing specific activity."

reach
"Pathogenic germline variants in the BRCA1 associated protein 1 (BAP1) gene cause the BAP1 tumor predisposition syndrome (BAP1-TPDS) with increased risk of several cancers, the most frequent of which is uveal melanoma (UM)."

reach
"While ubiquitination enzyme UBE2O monoubiquitinates the NLS of BAP1 and induces translocation (inactivation) of BAP1 from the nucleus to the cytoplasm, DUB activity of the UCH-domain of BAP1 counteracts the UBE2O activity mediated through the intramolecular interaction between the UCH-domain and COOH-terminal domain of BAP1."

reach
"Germline pathogenic variants in the BRCA1 associated protein-1 (BAP1) gene cause the BAP1 tumor predisposition syndrome (TPDS)."
BAP1 increases the amount of BAP1.
| 5
BAP1 increases the amount of BAP1. 5 / 5
| 5

reach
"Genetic mutations in BAP1 could modulate BAP1 protein expression or could alter BAP1 function in the absence of alterations in protein expression."

reach
"Deletion of chromosome 3 and BAP1 inactivating mutations cause loss of BAP1 expression and function in cancer."

reach
"BAP1-IP3R3 CoIP was barely detectable in BAP1 +/- fibroblasts; BAP1 rescue significantly increased the amount of BAP1 that CoIP with IP3R3 (XREF_FIG)."

reach
"We also detect the mRNA and protein expression level of BAP1, tumors with BAP1 plasmid transfected group exhibited higher BAP1 mRNA and protein expression compared with the control group, suggesting that BAP1 plasmid overexpression could rescue the expression of BAP1 suppressed by miR-31."

reach
"Mechanically, we show that BAP1 represses metabolic stress induced UPR and cell death through activating transcription factor 3 (ATF3) and C/EBP homologous protein (CHOP), and reveal that BAP1 binds to ATF3 and CHOP promoters and inhibits their transcription."
BAP1 decreases the amount of BAP1.
| 2
BAP1 decreases the amount of BAP1. 2 / 3
| 2

reach
"This result is perhaps surprising given that BAP1 inactivation or loss of BAP1 expression has been detected using IHC and whole-exome sequencing in approximately 25% of the intrahepatic CCAs [XREF_BIBR, XREF_BIBR, XREF_BIBR]."

reach
"Using patient derived lymphoblastoid cell lines, we found that carriers of pathogenic BAP1 germline variants (c. 852_del and c. 1358_1359del) had reduced expression of full-length BAP1, Vimentin and Snail, as compared to controls with wild-type BAP1 (BAP1 WT), whereas BAP1 germline VUS (c. 299T> C and c. 551A> G) or likely benign carriers were not significantly different from wild-type BAP1 WT."
ULD affects BAP1
| 19
| 15

sparser
"We have demonstrated that ASXL, via its AB box, acts as a molecular scaffold to recruit the BAP1-ULD domain to transcription factors that bind to specific target genes; the BAP1-UCH catalytic domain then removes ubiquitin from histones on chromatin to regulate the expression of these transcriptional targets ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"From our previous computer modeling studies, BAP1-ULD is predicted to form a few long helices, while ASXL-AB box is predicted to form five helices ( xref )."

sparser
"Biophysical and biochemical characterization of BAP1-UCH, BAP1-ULD, ASXL2-AB, and the UCH/ULD/AB complex."

sparser
"Using computer molecular modeling of UCHL5 structures, we predicted that the BAP1-ULD domain folds back to the BAP1-UCH catalytic domain and that the ASXL2-AB box stabilizes the UCH catalytic loop via a unique BAP1 mechanism not seen in other UCH proteins, allowing for ubiquitin to fit into the active site ( xref , xref )."

sparser
"The N-terminus of ASXL, which includes ASXH, binds the ULD domain of BAP1, allowing the UCH catalytic site to be brought in proximity to the target promoter."

sparser
"We next tested the affinity and kinetics of BAP1-ULD and ASXL2-AB using SPR."

sparser
"Upon loss of binding of the ASXL-AB box to BAP1-ULD, BAP1 enzymatic activity is abolished, resulting in disturbances in BAP1-dependent chromatin state/gene expression that contribute to the pathogenesis of human malignancy and other diseases."

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
BAP1 affects cell growth
| 17
BAP1 inhibits cell growth.
| 14
| 14

reach
"Restoration of BAP1 inhibits cell growth in cancer cell lines that lack BAP1 (NCI-H226 cells) or express defective BAP1 (769-P cells) [XREF_BIBR, XREF_BIBR]."

reach
"BAP1 knockdown in vitro has resulted in decreased cell proliferation and mediation of apoptosis in MSTO211H, HMeso, and H2373 mesothelioma cell lines, and reintroduction of wild-type BAP1 in BAP1-null cell line NCI-H226 promoted cell growth, yet another study reported that this was counterbalanced by increased apoptosis, indicating that the consequences of in vitro manipulation may be cell type dependent [XREF_BIBR, XREF_BIBR]."

reach
"BAP1 enhances BRCA1 mediated inhibition of breast cancer cell growth and is the first nuclear localized ubiquitin carboxy-terminal hydrolase to be identified."

reach
"With the colony formation assay, we also found that WT BAP1 suppressed the anchorage independent cell growth of the MM cells with BAP1 deletion, whereas mutant BAP1 showed little or no reduced effect."

reach
"BAP1 enhances BRCA1 mediated suppression of cell growth in colony formation assays, and this suppression by BAP1 is augmented by its UCH enzymatic domain."

reach
"BAP1 has been reported to suppress cell growth through its catalytic activity and nuclear localization XREF_BIBR XREF_BIBR."

reach
"BAP1 overexpression also suppressed cell growth in the 769-P renal cancer cell line in vitro XREF_BIBR."

reach
"Again, depletion of either BAP1 or KLF5 did not significantly decreased MDA-MB-231 cell growth (XREF_SUPPLEMENTARY)."

reach
"That study also showed that BAP1 slows cell growth through altered G1-S checkpoint regulation."

reach
"In the absence of a carcinogenic insult however, BAP1 loss may not promote cell growth."
BAP1 activates cell growth.
| 3
| 3

reach
"In this study, we showed that BAP1 knockdown decreased DNA synthesis, the number of S-phase cells and cell growth in HCC1806 cells."

reach
"In cutaneous melanoma cell lines, however, stable over-expression of BAP1 promoted cell growth while knockdown of BAP1 suppressed cell proliferation with concomitant decrease of survivin, an anti-apoptotic protein [17]."

reach
"When KLF5 is depleted, BAP1 failed to promote cell growth (XREF_SUPPLEMENTARY)."
BAP1 affects ULD
| 19
| 15

sparser
"We have demonstrated that ASXL, via its AB box, acts as a molecular scaffold to recruit the BAP1-ULD domain to transcription factors that bind to specific target genes; the BAP1-UCH catalytic domain then removes ubiquitin from histones on chromatin to regulate the expression of these transcriptional targets ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"From our previous computer modeling studies, BAP1-ULD is predicted to form a few long helices, while ASXL-AB box is predicted to form five helices ( xref )."

sparser
"Biophysical and biochemical characterization of BAP1-UCH, BAP1-ULD, ASXL2-AB, and the UCH/ULD/AB complex."

sparser
"Using computer molecular modeling of UCHL5 structures, we predicted that the BAP1-ULD domain folds back to the BAP1-UCH catalytic domain and that the ASXL2-AB box stabilizes the UCH catalytic loop via a unique BAP1 mechanism not seen in other UCH proteins, allowing for ubiquitin to fit into the active site ( xref , xref )."

sparser
"The N-terminus of ASXL, which includes ASXH, binds the ULD domain of BAP1, allowing the UCH catalytic site to be brought in proximity to the target promoter."

sparser
"We next tested the affinity and kinetics of BAP1-ULD and ASXL2-AB using SPR."

sparser
"Upon loss of binding of the ASXL-AB box to BAP1-ULD, BAP1 enzymatic activity is abolished, resulting in disturbances in BAP1-dependent chromatin state/gene expression that contribute to the pathogenesis of human malignancy and other diseases."

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
BAP1 affects MCRS1
2 | 8 7
BAP1 binds MCRS1.
2 | 3 7
2 | 3 7

sparser
"BAP1 binds to MCRS1 and stabilizes MCRS1 by de-ubiquitination."

reach
"Taken together, these results indicated that BAP1 forms a complex with MCRS1 in vivo."

reach
"BAP1 binds to MCRS1 and stabilizes MCRS1 by de-ubiquitination."

sparser
"Taken together, these results indicated that BAP1 forms a complex with MCRS1 ."

sparser
"Since BAP1MCRS1 interaction has not been reported in the literature, we aimed to investigate the potential functional relationship between BAP1 and MCRS1."

sparser
"To gain more insight into the BAP1MCRS1 interaction, we determined which region of MCRS1 mediated its interaction with BAP1."

sparser
"Next, we investigated whether endogenous BAP1 and MCRS1 can interact with each other."

No evidence text available

reach
"Since BAP1-MCRS1 interaction has not been reported in the literature, we aimed to investigate the potential functional relationship between BAP1 and MCRS1.To verify that MCRS1 is a bona fide BAP1 inte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We next determined the region of BAP1 that binds to MCRS1."
BAP1 activates MCRS1.
| 3
BAP1 activates MCRS1. 3 / 4
| 3

reach
"BAP1 also contributes to chromosome stability by binding the microspherule protein 1 (MCRS1) which plays an essential role in spindle assembly XREF_BIBR."

reach
"In the absence of de novo protein synthesis, the half-life of endogenous MCRS1 protein was much shorter in the BAP1 depleted cells than that in control cells, further suggesting that BAP1 regulates MC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"To determine whether BAP1 increases MCRS1 by extending its half-life, the protein level of MCRS1 was monitored after treatment with the protein synthesis inhibitor cycloheximide (CHX)."
BAP1 deubiquitinates MCRS1.
| 2
BAP1 deubiquitinates MCRS1. 2 / 2
| 2

reach
"Since BAP1 is a de-ubiquitinating enzyme, we explored whether BAP1 de-ubiquitinates and stabilizes MCRS1."

reach
"Moreover, de-ubiquitination of MCRS1 by BAP1 plays important roles in regulating proper mitotic progression and prevents chromosome instability.The yeast two-hybrid screen was performed with full leng[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
BAP1 affects INO80
1 | 15 1
BAP1 activates INO80.
| 6
BAP1 activates INO80. 6 / 7
| 6

reach
"One reason for this could be that the simultaneous knockdown of CHIP and BAP1 decreased Ino80 only marginally compared to the individual knockdown, which is likely due to a limitation of siRNA knockdown."

reach
"Ino80 is downregulated in BAP1 defective mesothelioma, owing to the lack of a BAP1 mediated Ino80 stabilization mechanism, thus raising the possibility of a tumorigenic role for INO80 in this cancer."

reach
"Conversely, knockdown of CHIP, BAP1, or both decreased Ino80 preferentially in the chromatin fractions (XREF_FIG)."

reach
"Importantly, Ino80 levels are often reduced in BAP1-null mesothelioma cells, which lack a BAP1 mediated Ino80 stabilization mechanism, and downregulated in BAP1 defective cancer cells in mesothelioma patients [XREF_BIBR]."

reach
"By recruiting INO80 to the stalled forks, BAP1 allows stress induced stalled replication forks to restart, a mechanism that suppresses genome instability and thus cancer development."

reach
"While individual expression of CHIP and BAP1 increased Ino80, as expected, coexpression of these proteins increased endogenous Ino80 (XREF_FIG) and transfected Flag-Ino80 beyond the levels achieved by individual expression of either protein (XREF_FIG)."
BAP1 deubiquitinates INO80.
| 6
BAP1 deubiquitinates INO80. 6 / 6
| 6

reach
"In line with this notion, BAP1 depletion resulted in reduced localization of RPA, RAD51 and BRCA1 to IRIF or microlaser irradiation site [100,101], although it remains ambiguous whether either histone[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In addition to MCRS1, a recent study revealed that BAP1 deubiquitinates and stabilizes Ino80, a key subunit of the INO80 chromatin remodeling complex implicated in DNA replication during stress."

reach
"BAP1 overexpression also abolished basal Ino80 ubiquitination (XREF_FIG and XREF_FIG, rightmost lanes)."

reach
"BAP1 interacts with and deubiquitylates INO80, playing a critical role in stabilizing and targeting the INO80 chromatin remodeling complex to the replication forks."

reach
"Since BAP1 deubiquitinates and stabilizes Ino80, we investigated the relationship between CHIP and BAP1 in Ino80 ubiquitination."

reach
"BAP1 deubiquitylates INO80 and thereby protects the protein from ubiquitin mediated proteolysis."
BAP1 binds INO80.
1 | 3 1
1 | 3 1

reach
"BAP1 interacts with and deubiquitylates INO80, playing a critical role in stabilizing and targeting the INO80 chromatin remodeling complex to the replication forks."

reach
"A recent study demonstrates that Ino80 interacts with BRCA1 associated protein-1 (BAP1), a tumor suppressor that also stabilizes Ino80, in normal DNA replication [XREF_BIBR]."

reach
"Both BAP1 and INO80 bind chromatin at replication forks upon HU treatment."

sparser
"Both BAP1 and INO80 bind chromatin at replication forks upon HU treatment."

No evidence text available
BAP1 affects ASXL1-MT
| 18
BAP1 binds ASXL1-MT.
| 13
BAP1 binds ASXL1-MT. 10 / 13
| 13

reach
"In addition to these reports implicating BAP1 in myeloid transformation, the present results identify the underlying molecular mechanisms by which ASXL1-MT and BAP1 complex induce myeloid transformation; BAP1 plays a necessary role in maintaining aberrant posterior HOXA expression in ASXL1-mutant leukemia and in sustaining their leukemic proliferation."

reach
"These data indicate that the ASXL1-MT and BAP1 complex induces upregulation of posterior HOXA genes and IRF8 via removal of H2AK119ub."

reach
"The ASXL1-MT and BAP1 complex enhances transient myeloid proliferation, but does not increase the long-term proliferation potential of murine and human HSPCs."

reach
"This hyperactive ASXL1-MT and BAP1 complex promotes aberrant myeloid differentiation of haematopoietic progenitor cells and accelerates RUNX1-ETO-driven leukaemogenesis."

reach
"Mechanisms underlying the enhancement of the catalytic activity of ASXL1-MT and BAP1 complex warrant further investigation."

reach
"Monoubiquitinated ASXL1-MT in turn enhances the catalytic activity of BAP1, creating a positive feedback loop that establishes the hyperactive ASXL1-MT and BAP1 complex."

reach
"Together, these results indicate that the ASXL1-MT and BAP1 complex impairs terminal differentiation of HSPCs, except for differentiation toward monocytes."

reach
"Cells expressing ASXL1-MT and BAP1 did not produce colonies beyond the fourth passage, suggesting that the ASXL1-MT and BAP1 complex itself is not sufficient to transform murine HSPCs."

reach
"The ASXL1-MT and BAP1 complex is not a simple growth-accelerator, but rather inhibits terminal differentiation of HSPCs toward granulocytes, macrophages, mast cells, and erythrocytes."

reach
"Thus, Irf8 is also a downstream target of the ASXL1-MT and BAP1 complex, which is likely to underlie the enhanced monocytic differentiation of HSPCs coexpressing ASXL1-MT and BAP1."
BAP1 ubiquitinates ASXL1-MT.
| 3
BAP1 ubiquitinates ASXL1-MT. 3 / 3
| 3

reach
"We have shown here that BAP1 recruits ubiquitin ligases to induce both mono- and polyubiquitination of ASXL1-MT."

reach
"Thus, BAP1 enhances monoubiquitination of ASXL1-MT probably through the recruitment of ubiquitin ligases and the concurrent removal of the polyubiquitin chain from it."

reach
"BAP1 expression results in increased monoubiquitination of ASXL1-MT, which in turn increases the catalytic function of BAP1."
BAP1 activates ASXL1-MT.
| 2
BAP1 activates ASXL1-MT. 2 / 2
| 2

reach
"In the mast cell differentiation assay, ASXL1-MT alone modestly impaired mast cell maturation of murine HSPCs, and this inhibitory effect was enhanced by BAP1 coexpression."

reach
"However, UBE2O depletion did not inhibit the BAP1 induced mobility shift of ASXL1-MT, indicating involvement of other ligases promoting monoubiquitination of ASXL1-MT."
BAP1 affects Ubiquitin
| 6 10
BAP1 binds Ubiquitin.
| 2 10
| 2 10

sparser
"The CTD of BAP1 interacts with monoubiquinated DEBAUD domain, which allosterically increases BAP1’s affinity for ubiquitin on H2A at Lys-119 H2A and stimulates deubiquitination [ xref , xref ]."

reach
"Thus, we hypothesized that Ub binding by the BAP1/DEUBAD complex without actual catalysis is important for DEUBAD monoubiquitination and we sought to investigate this possibility."

sparser
"The thermodynamic analysis reveals that BAP1ubiquitin interaction is entropically driven."

sparser
"Thus it seems likely that ASXL1 DEU also uses this ubiquitin interface to stabilize ubiquitin binding of BAP1 and stimulate activity."

sparser
"We have predicted a few residues in BAP1N that might play an important role in BAP1ubiquitin binding based on the ubiquitin-binding interface of analogous UCHs."

sparser
"Our comparative thermodynamic analysis reveals that BAP1ubiquitin interaction is majorly driven by entropy factor which is unique amongst UCHs."

reach
"BAP1 Ub binding mutants, Y33A/I226A/F228A, L8A/F168A/N229A, and L35A/I214A/F228A/L230A were generated using gene synthesis (BioBasic) and then subcloned into a modified pENTR D-Topo plasmid (Life Technologies)."

sparser
"However, mechanistic insights into BAP1ubiquitin interaction remain enigmatic."

sparser
"In the present work, we have shown how BAP1 interacts with the ubiquitin by studying the enzyme kinetics and thermodynamics of BAP1N–ubiquitin interaction."

sparser
"In this complex, BAP1 is the catalytic subunit and its activity is strongly dependent on the interaction with conserved DEUBiquitinase Adaptor (DEUBAD) domain of ASXLs that induces conformational changes able to increase BAP1 affinity for ubiquitin [ xref ]."
BAP1 activates Ubiquitin.
| 4
| 4

reach
"The BAP1 gene produces a 729 AA protein containing several functional domains: a ubiquitin C-terminal hydrolase (UCH) domain, a BRCA1-associated RING domain protein 1 (BARD1) binding domain, an HCF-C1 binding motif (HBM) and a nuclear localization signal ."

reach
"BAP1 and the BRCA1 and BARD1 complex BRCA1 participates in several multiprotein complexes, and plays roles in : repair of double stranded DNA (dsDNA) breaks by homologous recombination; chromatin remo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The deubiquitinase (DUB) and tumor suppressor BAP1 catalyzes ubiquitin removal from histone H2A Lys 119 and coordinates cell proliferation, but how BAP1 partners modulate its function remains poorly understood."

reach
"As part of the Polycomb repression machinery, BAP1 is activated by the deubiquitinase adaptor domain of ASXL1 mediating gene repression by cleaving ubiquitin (Ub) from histone H2A in nucleosomes."
| 1 10 1

reach
"As a deubiquitinating enzyme, BAP1 contributes to gene transcription, cell differentiation, DNA damage repair, apoptosis, and cell metabolism in tumor inhibition."

reach
"In addition, melanoma specific network analysis followed by Kaplan-Meier analysis along with log-rank tests identified tyrosinase, hedgehog acyltransferase, BRCA1 associated protein 1 and melanocyte inducing transcription factor as potential therapeutic targets for melanoma."

reach
"These included the SETD1A, KMT2C and MLL3, KMT2E and MLL5, and KMT2A and MLL1 methyltransferases that install activating histone H3-lysine 4 trimethylation (H3K4me3) marks; OGT (O-GlcNac transferase), a protein that modulates multiple cell pathways but more recently has been implicated in promoting promoter occupancy of RNAPII; and BAP1 (BRCA1 Associated Protein 1), a deubiquitinase that stimulates transcription, at least in part, by removing repressive histone H2A-K119 monoubiquitin."

reach
"These data indicate that BaP1 triggers transcription mechanisms in FLSs that result in an increase in COX-2 protein content."

reach
"The observation that BaP1 induced translocation of the NF-κB p50 and RelA (p65) subunits into the nucleus gives further support to the hypothesis that this transcription factor is activated by BaP1 and provides a mechanism for this activation."

reach
"p53 binds to the SLC7A11 promoter, directly suppressing its transcription; the nuclear deubiquitinase (DUB) BAP1 represses SLC7A11 transcription by removing histone 2A ubiquitination from the SLC7A11 promoter; and KEAP1 represses SLC7A11 transcription through degrading NRF2, a master transcription factor of antioxidant response and regulator of SLC7A11."

eidos
"Taken together , these results establish that PRC1 is epistatic to BAP1.com and that BAP1 promotes transcription by counteracting PRC1-mediated H2A ubiquitination ( Fig. 7 ) ."

reach
"Previously, we found that hypoxia-inducible factor 2α (HIF2α) and BAP1 activate interferon-stimulated gene factor 3 (ISGF3), a transcription factor activated by type I interferons and a tumor suppressor in ccRCC xenograft models."

reach
"BAP1 activates transcription in an enzymatic-activity-dependent manner and regulates the expression of a variety of genes involved in numerous cellular processes."

reach
"RNA sequencing and chromatin immunoprecipitation coupled with quantitative PCR analyses revealed that reduced BAP1 expression suppressed upregulation of the transcription factors AP-1 and EGR1/2, as well as myeloid dysplasia associated genes, by retarding H2AK119Ub removal caused by ASXL1 mutation."

reach
"In general, various molecules regulate SLC7A11, such as tumor suppressor P53, tumor suppressor BRCA1-associated protein 1 (BAP1), activating transcription factor 3/4 (ATF3/ATF4), beclin 1 (BECN1), and nuclear factor erythroid 2-related factor 2 (NRF2) (Figure 3)."

eidos
"Moreover , ZEB1 promoted down-regulation of crucial genes involved in melanocyte differentiation , including BAP1 , melanocyte inducing transcription factor ( MITF9 ) , tyrosinase ( TYR ) , and tyrosinase-related protein 1 ( TYRP ) ."

eidos
"Similarly , BAP1 , an epigenetic regulator and tumor suppressor mutated in various types of cancer , can downregulate the transcription of SLC7A11 and thus potentiate ferroptosis ( Zhang et al ., 2018 ) ."

eidos
"Under glucose starvation , BAP1 can directly bind to the promoter of ATF3 and CHOP , inhibit its transcription , and thus inhibit the UPR and cell death induced by the ER stress ( Figure 2p ) ."
BAP1 affects OGT
4 1 | 8 1
BAP1 binds OGT.
4 | 3 1
4 | 1 1

No evidence text available

No evidence text available

reach
"Knock-in mice expressing BAP1 with a 3xFlag tag revealed that BAP1 interacts with HCF-1, OGT, and the polycomb group proteins ASXL1 and ASXL2 in vivo."

No evidence text available

sparser
"BAP1 interacts with OGT in the PR-DUB complex, and our data suggest that the PR-DUB complex plays a multifunctional role in repression of the injury program."

No evidence text available
HCFC1 binds OGT and BAP1. 2 / 2
| 2

reach
"The fact that HCF-1 acts as an important modulator of cellular circadian rhythm [XREF_BIBR] raises the possibility that the OGT, HCF-1, and BAP1 complex interacts with the BMAL1, CLOCK, and PER complex to modulate circadian rhythm."

reach
"Within the BAP1/HCF-1/OGT complex, HCF1 is modified by O-GlcNacylation, the addition of single O-linked N-acetylglucosamine moiety to specific serine or threonine residues (50,51)."
BAP1 increases the amount of OGT.
| 3
BAP1 increases the amount of OGT. 3 / 4
| 3

reach
"BAP1 also increases OGT protein levels, suggesting that BAP1 is a potential deubiquitinase for OGT as well (XREF_SUPPLEMENTARY)."

reach
"OGT and HCF-1 levels were decreased by Bap1 deletion, indicating a critical role for BAP1 in stabilizing these epigenetic regulators."

reach
"OGT and HCF-1 levels were decreased by Bap1 deletion, and were associated with myeloid transformation ."
BAP1 deubiquitinates OGT.
1 | 2
BAP1 deubiquitinates OGT. 3 / 3
1 | 2

reach
"XREF_BIBR, XREF_BIBR, XREF_BIBR BAP1 also deubiquitylates OGT and regulates its levels."

reach
"To investigate whether BAP1 can deubiquitylate and stabilize OGT directly, rather than decreased OGT being secondary to a reduction in HCF-1, we affinity purified ubiquitylated and Myc tagged human OGT from HEK293T cells, and then incubated it with human BAP1 and human ASXL1 residues 2-365 co-purified from Sf9 cells."

"The tumor suppressor BAP1 associates with ASXL1/2 to form the core Polycomb complex PR-DUB, which catalyzes the removal of mono-ubiquitin from several substrates including histone H2A. This complex also mediates the poly-deubiquitination of HCFC1, OGT and PCG1-α, preventing them from proteasomal degradation."
Ubiquitin affects BAP1
| 4 10
Ubiquitin binds BAP1.
| 2 10
| 2 10

sparser
"The CTD of BAP1 interacts with monoubiquinated DEBAUD domain, which allosterically increases BAP1’s affinity for ubiquitin on H2A at Lys-119 H2A and stimulates deubiquitination [ xref , xref ]."

reach
"Thus, we hypothesized that Ub binding by the BAP1/DEUBAD complex without actual catalysis is important for DEUBAD monoubiquitination and we sought to investigate this possibility."

sparser
"The thermodynamic analysis reveals that BAP1ubiquitin interaction is entropically driven."

sparser
"Thus it seems likely that ASXL1 DEU also uses this ubiquitin interface to stabilize ubiquitin binding of BAP1 and stimulate activity."

sparser
"We have predicted a few residues in BAP1N that might play an important role in BAP1ubiquitin binding based on the ubiquitin-binding interface of analogous UCHs."

sparser
"Our comparative thermodynamic analysis reveals that BAP1ubiquitin interaction is majorly driven by entropy factor which is unique amongst UCHs."

reach
"BAP1 Ub binding mutants, Y33A/I226A/F228A, L8A/F168A/N229A, and L35A/I214A/F228A/L230A were generated using gene synthesis (BioBasic) and then subcloned into a modified pENTR D-Topo plasmid (Life Technologies)."

sparser
"However, mechanistic insights into BAP1ubiquitin interaction remain enigmatic."

sparser
"In the present work, we have shown how BAP1 interacts with the ubiquitin by studying the enzyme kinetics and thermodynamics of BAP1N–ubiquitin interaction."

sparser
"In this complex, BAP1 is the catalytic subunit and its activity is strongly dependent on the interaction with conserved DEUBiquitinase Adaptor (DEUBAD) domain of ASXLs that induces conformational changes able to increase BAP1 affinity for ubiquitin [ xref ]."
Ubiquitin inhibits BAP1.
| 2
| 2

reach
"The fact that an ubiquitin conjugate can inhibit BAP1 to a greater extent than ubiquitin alone, illustrates how this DUB prefers binding its target (ubiquitin conjugate) over its product (ubiquitin alone)."

reach
"ASXL1 WT could induce H2AK119 ubiquitination (Ub) through negatively regulating BAP1 which removes ubiquitination of H2AK119 as a deubiquitinase (Fig. 2b)."
ASXL3 affects BAP1
2 | 7 5
ASXL3 binds BAP1.
2 | 5 4
2 | 5 4

reach
"While ASXL1 and ASXL2 were known to interact with BAP1 from proteomics studies XREF_BIBR XREF_BIBR, ASXL3 was not detected as a BAP1 binding partner, possibly because of the low levels of BAP1 and ASXL3 complexes in cells."

No evidence text available

No evidence text available

reach
"ASXL3 interacts with BAP1, a ubiquitin terminal hydroxylase and removes the mono-ubiquitin (Ub1) from the H2AK119Ub1 [XREF_BIBR]."

reach
"It suggests that there may be a functional association between BAP1 and ASXL3 in cells and further cell based work is required to shed more light on this issue."

reach
"We found that ASXL3 interacts with BAP1, a hydrolase that removes mono-ubiquitin from histone H2A lysine 119 (H2AK119Ub1) as a component of the Polycomb repressive deubiquitination (PR-DUB) complex."

sparser
"We found that ASXL3 interacts with BAP1, a hydrolase that removes mono-ubiquitin from histone H2A lysine 119 (H2AK119Ub1) as a component of the Polycomb repressive deubiquitination (PR-DUB) complex."

sparser
"Interestingly, the truncated ASXL3 protein interacts with BAP1 protein ( xref )."

sparser
"ASXL3 interacts with BAP1, a ubiquitin terminal hydroxylase and removes the mono-ubiquitin (Ub1) from the H2AK119Ub1 [ xref ]."

sparser
"Our in vitro analysis indicates that also ASXL3 can bind and activate BAP1, which is not surprising in the light of the high sequence similarity between the DEUBAD domains in the ASXL family."
ASXL3 activates BAP1.
| 2 1
ASXL3 activates BAP1. 3 / 3
| 2 1

sparser
"Our in vitro analysis indicates that also ASXL3 can bind and activate BAP1, which is not surprising in the light of the high sequence similarity between the DEUBAD domains in the ASXL family."

reach
"The DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing its affinity for ubiquitin."

reach
"In this paper we describe that similar to the homologous UCH-L5 and RPN13 DEU complex, the DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing BAP1 's affinity specifically for the ubiquitin in the substrate, through a combination of mild effects."
ASXL1-MT affects BAP1
| 15
ASXL1-MT binds BAP1.
| 13
BAP1 binds ASXL1-MT. 10 / 13
| 13

reach
"In addition to these reports implicating BAP1 in myeloid transformation, the present results identify the underlying molecular mechanisms by which ASXL1-MT and BAP1 complex induce myeloid transformation; BAP1 plays a necessary role in maintaining aberrant posterior HOXA expression in ASXL1-mutant leukemia and in sustaining their leukemic proliferation."

reach
"These data indicate that the ASXL1-MT and BAP1 complex induces upregulation of posterior HOXA genes and IRF8 via removal of H2AK119ub."

reach
"The ASXL1-MT and BAP1 complex enhances transient myeloid proliferation, but does not increase the long-term proliferation potential of murine and human HSPCs."

reach
"This hyperactive ASXL1-MT and BAP1 complex promotes aberrant myeloid differentiation of haematopoietic progenitor cells and accelerates RUNX1-ETO-driven leukaemogenesis."

reach
"Mechanisms underlying the enhancement of the catalytic activity of ASXL1-MT and BAP1 complex warrant further investigation."

reach
"Monoubiquitinated ASXL1-MT in turn enhances the catalytic activity of BAP1, creating a positive feedback loop that establishes the hyperactive ASXL1-MT and BAP1 complex."

reach
"Together, these results indicate that the ASXL1-MT and BAP1 complex impairs terminal differentiation of HSPCs, except for differentiation toward monocytes."

reach
"Cells expressing ASXL1-MT and BAP1 did not produce colonies beyond the fourth passage, suggesting that the ASXL1-MT and BAP1 complex itself is not sufficient to transform murine HSPCs."

reach
"The ASXL1-MT and BAP1 complex is not a simple growth-accelerator, but rather inhibits terminal differentiation of HSPCs toward granulocytes, macrophages, mast cells, and erythrocytes."

reach
"Thus, Irf8 is also a downstream target of the ASXL1-MT and BAP1 complex, which is likely to underlie the enhanced monocytic differentiation of HSPCs coexpressing ASXL1-MT and BAP1."
ASXL1-MT activates BAP1.
| 2
ASXL1-MT activates BAP1. 2 / 2
| 2

reach
"Overexpression of ASXL1-MT promoted nuclear import of BAP1, which was observed only weakly when we used the ubiquitination deficient ASXL1-MT-K351R."

reach
"Together, these data suggest that ASXL1-MT, especially the monoubiquitinated form of ASXL1-MT, increases catalytic function of BAP1."
BAP1 affects cell cycle
| 2 12
BAP1 activates cell cycle.
| 8
| 7

reach
"In functional experiments, BAP1 has been shown to suppress lung cancer tumorigenesis in athymic nude mice, promote the cell cycle and induce both apoptosis and necrosis."

reach
"In sharp contrast, RNA interference mediated depletion of BAP1 induced cell cycle progression defects and inhibited cell proliferation in vitro XREF_BIBR XREF_BIBR, suggesting that BAP1 may have dual roles in cancer development."

reach
"The E2F1 targets pathway was involved in the BAP1-mediated cell cycle progression of uveal melanoma cells [3]."

reach
"In the spleen, Bap1 deletion reduced cell cycle proteins (p = 6.2e-20), consistent with pancytopenia often observed in myelodysplastic disorders (XREF_FIG)."

reach
"BAP1 loss causes transient cell cycle inhibition."

reach
"Moreover, RNA mediated depletion of BAP1 promoted cell cycle G1-S progression XREF_BIBR."

reach
"Downregulation of BAP1 promotes proliferation, cell cycle progression, and invasion of ICC in vitro."
BAP1 bound to HCFC1 and KLF5 activates cell cycle. 1 / 1
| 1

reach
"To test whether the KLF5, BAP1, and HCF complex promotes cell cycle progression through p27, we knocked down p27 together with KLF5, BAP1, HCF-1 or OGT (XREF_FIG)."
BAP1 inhibits cell cycle.
| 2 4
| 2 4

eidos
"These results indicated that BAP1 suppressed ICC cell proliferation , cell cycle progression , and invasion via inhibiting ERK1 / 2 and JNK / c-Jun signaling pathways ."

reach
"These results indicated that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways.To further validate these findings, we used inhibitors of the ERK1/2 pathway (U0126) and JNK/c-Jun pathway (SP600125) in RBE-shBAP1 cells."

reach
"XREF_BIBR reported that BAP1 overexpression in the BAP1-null NCI-H226 human non-small-cell lung cancer cell line suppressed growth in DUB activity- and nuclear localization dependent manners by accelerating the cell cycle G1/S transition and inducing cell death."

reach
"Interestingly, there was no substantial difference in cell viability, BrdU incorporation or cell cycle profile between BAP1 deficient and control cells after stable expression of the shRNA constructs for at least 14days, indicating that the initial cell cycle inhibition caused by BAP1 depletion was transient."

eidos
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation , cell cycle progression , and invasion via inhibiting ERK1 / 2 and JNK / c-Jun signaling pathways ."

reach
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways."
BAP1 affects H2Aub
| 1 13
BAP1 inhibits H2Aub.
| 1 4
BAP1 inhibits H2Aub. 5 / 5
| 1 4

reach
"Monoubiquitinated histone H2A, H2Aub (K118 in Drosophila or K119 in vertebrates) is catalyzed by the Polycomb group (PcG) repressive complex 1 (PRC1) and reversed by the PcG-repressive deubiquitinase (PR-DUB) and BAP1 complex."

reach
"Genomic analyses together with robust statistics have revealed that the restoration of BAP1 facilitates the formation of the polycomb repressive deubiquitinase (PR-DUB) complex and inhibits ubiquitinated histone 2A (H2Aub) occupancy on the SLC7A11 promoter."

eidos
"Our own findings suggest that BAP1 inhibition leads to increased level of H2Aub , which interferes with chromatin and histone modifications at double-strand breaks ."

reach
"Analysis of our H2Aub ChIP-seq data revealed that restoration of BAP1 WT, but not BAP1 C91A, markedly decreased H2Aub occupancy at both the promoter and gene body of SLC7A11 (XREF_FIG), which was further confirmed by H2Aub ChIP assay on the SLC7A11 promoter and representative exons (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"Several studies have revealed that BAP1 can inhibit ubiquitinated histone 2A (H2Aub) occupancy on the SLC7A11 promoter (Zhang et al., 2018)."
BAP1 decreases the amount of H2Aub.
| 4
BAP1 decreases the amount of H2Aub. 4 / 4
| 4

reach
"SLC7A11 appears to be a direct target of BAP1, which reduces the levels of H2Aub on SLC7A11 promoter, but yet represses its transcriptional activity."

reach
"As the expression of BAP1, but not its catalytic dead form, is known to reduce the global levels of H2Aub, this strategy has been used to capture genomic locations that exhibit higher levels of H2Aub using chromatin immunoprecipitation in combination with next-generation genome sequencing (ChIP-Seq) ."

reach
"Our own findings suggest that BAP1 inhibition leads to increased level of H2Aub, which interferes with chromatin and histone modifications at double-strand breaks."

reach
"To test this hypothesis, we conducted gene set enrichment analysis (GSEA) on the genes with different fold changes in BAP1 dependent H2Aub reduction (5837 genes whose H2Aub levels were reduced by BAP1 by> 1.6-fold, 1494 genes by> 2-fold, and 101 genes by> 2.5-fold)."
BAP1 deubiquitinates H2Aub.
| 3
BAP1 deubiquitinates H2Aub. 3 / 3
| 3

reach
"Other studies in mammalian cells have also shown that BAP1 represses the expression of some target genes with BAP1 dependent deubiquitination of H2Aub on the corresponding gene promoters XREF_BIBR, XREF_BIBR."

reach
"In conclusion, we found that BAP1 can deubiquitinate H2Aub and induces radioresistance in HNSCC cells via modulation of HR."

reach
"BAP1 deubiquitinates H2Aub."
BAP1 increases the amount of H2Aub.
| 2
BAP1 increases the amount of H2Aub. 2 / 2
| 2

reach
"Few thousands genomic locations have been inferred as potentially regulated by BAP1 owing to changes of H2Aub  levels."

reach
"In addition, BAP1 KO in UMSCC47 cells led to increased levels of H2Aub as compared with control cells (XREF_FIG)."
| 14
| 7

reach
"BAP1 promotes progenitor cell differentiation into melanocytes by increasing MITF expression and BAP1 loss may promote metastasis in UM by remodeling the epigenome to resemble that of stem-like migratory neural crest cells."

reach
"BAP1 knockout in mice leads to MM growth, larger tumors and increased number of metastases and BAP1 protein expression is also correlated with poor prognosis and shorter survival in RCC and cholangiocarcinoma, as well as UM (22–25)."

reach
"Early changes resulting in gain of chromosome 8q may activate macrophage infiltration, while sequential loss of BAP1 expression seems to drive T cell infiltration in UM."

reach
"Therefore, we hypothesized that decreasing BAP1 might increase the ability of UM cells to perform TEM in our model system."

reach
"Loss of BAP1 enhances UM transmigration."

reach
"Next, we used movie analysis of living cells to examine BAP1 depleted UM cells interacting with EC monolayers."

reach
"Interestingly, recent research has revealed that the gain of chromosomal 8q may worsen prognosis by activating macrophage infiltration, and the loss of BAP1 expression may drive T cell infiltration in UM (Gezgin et al., 2017), suggesting that immune infiltration plays a crucial role in the prognosis of UM.Emerging evidence shows that the immune microenvironment is crucial for cancer progression and response to therapeutics (Quail and Joyce, 2013)."
| 7

reach
"XREF_BIBR UM in BAP1 germ line mutants is usually diagnosed between the ages of 30 and 59 years, and is driven by inactivating mutations in the lone functional BAP1 gene, analogous to the frequent loss of chromosome 3 observed in high-risk sporadic disease."

reach
"However, the molecular mechanisms through which BAP1 promotes UM metastasis is still unclear.An important open question is whether the sunlight exposure could cooperate with BAP1 inactivation in UM development and progression."

reach
"BAP1 loss-of-function mutations promote the metastatic spread of UM cancers in patients XREF_BIBR, and TEM should be a rate limiting step of metastasis."

reach
"BAP1 depletion appears to promote UM cell migration underneath the monolayer."

reach
"The results suggest that knowledge of mutations in BAP1 and EIF1AX can enhance prognostication of UM beyond that determined by chromosome 3 and tumor characteristics."

reach
"Nevertheless, the key node of BAP1 pathway in driving or mediating UM metastasis remains unexplored.LaFave LM et al. reported that knockout of BAP1 results in elevated transcription of EZH2 gene in acute myeloid leukemia mouse bone marrow cells and human mesothelioma cells [48]."

reach
"This technique has generated a new and expanded list of BAP1 targets in UM that provides important insight into metastasis pathways and identifies novel potential therapeutic targets."
MiR-31 affects BAP1
| 13
MiR-31 decreases the amount of BAP1.
| 6
Modified miR-31 decreases the amount of BAP1. 3 / 3
| 3

reach
"Increased expression of miR-31 reduced the BAP1 expression, leading to cell proliferation and suppressed apoptosis [XREF_BIBR]."

reach
"Inhibition of miR-31 expression enhanced the level of BAP1 in the two cell lines."

reach
"Thus, the 1.5-2 fold increases of miR-31 levels in lung cancer tissues are sufficient to reduce the expression of BAP1 protein in vivo."
MiR-31 decreases the amount of BAP1. 3 / 3
| 3

reach
"In this study, we identified BAP1 as a direct target gene of miR-31 and showed that miR-31 inhibited BAP1 expression."

reach
"As anticipated, the expression of the BAP1 protein was significantly reduced by the introduction of miR-31, whereas the miR-31 inhibitor significantly increased the BAP1 protein levels in A549 cells."

reach
"We also detect the mRNA and protein expression level of BAP1, tumors with BAP1 plasmid transfected group exhibited higher BAP1 mRNA and protein expression compared with the control group, suggesting that BAP1 plasmid overexpression could rescue the expression of BAP1 suppressed by miR-31."
MiR-31 inhibits BAP1.
| 3
MiR-31 inhibits BAP1. 3 / 3
| 3

reach
"Finally, the cells transfected with miR-31 and the BAP1 overexpression plasmid exhibited significantly lower proliferation rates compared with the cells transfected with miR-31 and the control plasmid, suggesting that miR-31-resistant BAP1 could rescue the suppression of BAP1 by miR-31 and attenuate the proliferative effect of miR-31."

reach
"Tumors with both miR-31 and BAP1 overexpression exhibited significantly higher levels of BAP1 compared to tumors with miR-31 overexpression, suggesting that BAP1 overexpression could rescue the BAP1 suppression caused by miR-31."

reach
"BAP1 suppresses lung cancer progression and is inhibited by miR-31."
MiR-31 binds BAP1.
| 2
BAP1 binds miR-31. 2 / 2
| 2

reach
"These results indicate that miR-31 binds to BAP1 3 '-UTR directly and negatively regulates BAP1 expression in cervical cancer cells."

reach
"The aim of this study was to evaluate the interaction between BAP1 and miR-31 in MPM and their prognostic role in MPM."
MiR-31 activates BAP1.
| 2
MiR-31 activates BAP1. 2 / 2
| 2

reach
"These results indicated that miR-31 directly targeted BAP1 and induced EMT like changes in cervical cancer."

reach
"In summary, we demonstrated that miR-31 can induce EMT by downregulating BAP1 in cervical cancer, and miR-31-induced acceleration of cellar migration and invasion was partially by its regulation of BAP1 in vitro and in vivo."
MCRS1 affects BAP1
2 | 3 7
2 | 3 7

sparser
"BAP1 binds to MCRS1 and stabilizes MCRS1 by de-ubiquitination."

reach
"Taken together, these results indicated that BAP1 forms a complex with MCRS1 in vivo."

reach
"BAP1 binds to MCRS1 and stabilizes MCRS1 by de-ubiquitination."

sparser
"Taken together, these results indicated that BAP1 forms a complex with MCRS1 ."

sparser
"Since BAP1MCRS1 interaction has not been reported in the literature, we aimed to investigate the potential functional relationship between BAP1 and MCRS1."

sparser
"To gain more insight into the BAP1MCRS1 interaction, we determined which region of MCRS1 mediated its interaction with BAP1."

sparser
"Next, we investigated whether endogenous BAP1 and MCRS1 can interact with each other."

No evidence text available

reach
"Since BAP1-MCRS1 interaction has not been reported in the literature, we aimed to investigate the potential functional relationship between BAP1 and MCRS1.To verify that MCRS1 is a bona fide BAP1 inte[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We next determined the region of BAP1 that binds to MCRS1."
BAP1 affects DNA repair
| 4 9
| 4 9

reach
"Most of them are involved in DNA repair signaling, including MLH1, BRCA1/2, MUTYH, ATM, PMS2, MSH6, BAP1, and FANCA."

reach
"Indeed, by using a green fluorescent protein (GFP)-based reporter assay employing a recognition site for the rare-cutting I-SceI endonuclease for induction of DSBs , we found that loss of BAP1 caused a substantial reduction of GFP-positive cells, indicating loss of proper DNA repair (Supplementary Fig. 5k)."

reach
"BAP1 can deubiquitylate Ub-H2AK119 at the site of UV-induced DNA damage, and promote DNA repair by regulating the accumulation of repair proteins (BRCA1, RAD51, and RPA) (7)."

reach
"BAP1 promotes double-strand DNA repair by homologous recombination (HR), a key process to reduce genetic damage and prevent cancer."

reach
"Reduced BAP1 levels impair DNA repair as well as different forms of cell death and induce metabolic alterations that together favor cancer development and growth."

eidos
"BAP1 can deubiquitylate Ub-H2AK119 at the site of UV-induced DNA damage , and promote DNA repair by regulating the accumulation of repair proteins ( BRCA1 , RAD51 , and RPA ) ( 7 ) ."

eidos
"Reduced BAP1 levels impair DNA repair ( 11 ) as well as different forms of cell death ( 3 , 12 ) and induce metabolic alterations ( 13-15 ) that together favor cancer development and growth ."

reach
"Although LOH of additional genes within Chr 3p21.1 may contribute to tumor suppression, and loss of BAP1 in pancreatic cancer may be secondary—rather than the cause of genomic instability—our mouse model suggests Bap1 ablation suffices to cause defective DNA repair, pancreatitis, and cooperates with oncogenic Kras to promote pancreatic cancer."

reach
"Interestingly, BRCA1 proteins were decreased in the MM cells with BAP1 deletion, and transduction of the mutants as well as WT BAP1 increased BRCA1 proteins, suggesting that BAP1 may promote DNA repair partly through stabilizing BRCA1."

reach
"In heterozygous BAP1 +/- conditions, as in family members affected by the BAP1 cancer syndrome, the reduced BAP1 levels impair both DNA repair, making their cells accumulate more DNA damage, and the apoptotic response."
ATM affects BAP1
| 6 7
ATM phosphorylates BAP1.
| 5 6
ATM phosphorylates BAP1. 10 / 11
| 5 6

sparser
"BAP1 is phosphorylated by ATM kinase and promotes repair of DSBs through PAR-polymerase-dependent recruitment of the polycomb DUB complex to DNA damage sites [76] ."

sparser
"Intriguingly, BAP1 is directly phosphorylated by ATM upon DNA damage and is required for efficient assembly of homologous recombination (HR) repair factors BRCA1 and RAD51 at IR-induced foci to promote repair of DNA double-strand breaks (DSBs) xref , xref ."

reach
"BAP1 is phosphorylated by ATM kinase and promotes repair of DSBs through PAR-polymerase-dependent recruitment of the polycomb DUB complex to DNA damage sites [76]."

sparser
"It was further demonstrated that both of the catalytic activity and the phosphorylation of BAP1 by ATM is required for efficient assembly of the homologous recombination (HR) factors BRCA1 and RAD51 at the DSB loci (Fig. xref ) xref ."

reach
"BAP1 is phosphorylated by ATM kinase, and promotes repair of DSBs through PARP dependent recruitment of the polycomb DUB complex to DNA damage sites [XREF_BIBR]."

reach
"BAP1 was shown to mediate poly (ADP-ribose)-dependent recruitment of the Polycomb-repressive deubiquitinase complex (PR-DUB) to sites of DNA damage, was phosphorylated by ATM, and promoted repair of DNA double-strand breaks ."

reach
"Intriguingly, BAP1 is directly phosphorylated by ATM upon DNA damage and is required for efficient assembly of homologous recombination (HR) repair factors BRCA1 and RAD51 at IR-induced foci to promote repair of DNA double-strand breaks (DSBs) ."

sparser
"BAP1 is phosphorylated by ATM, which promotes its recruitment to the DSB site."

sparser
"Interestingly, during the cellular response to DNA double-strand breaks (DSBs) induced by UV irradiation or DNA damaging agents xref , BAP1 could be phosphorylated by DDR kinase ATM, and then recruited to the chromatin near DSB sites xref , xref ."

sparser
"BAP1 is phosphorylated by ATM kinase, and promotes repair of DSBs through PARP-dependent recruitment of the polycomb DUB complex to DNA damage sites [ xref ]."
ATM binds BAP1.
| 1 1
| 1 1

reach
"8 BAP1 also interacts with ataxia telangiectasia mutated and ataxia telangiectasia and Rad3 related proteins, which regulate the DNA damage induced by UV radiation."

sparser
"Further large germline studies of biliary tract cancers are warranted to better assess the association of ATM and BAP1 with this rare cancer type."
BAP1 affects Histone
| 7 3
BAP1 binds Histone.
| 2 3
| 2 1

sparser
"ASXL3 is less well studied than its paralogs but was shown to promote BAP1 binding to its histone substrate like its paralogs [ xref ]."
| PMC

reach
"Complex formation between BAP1 and the cell cycle regulator, HCF-1, bridges HCF-1 to histone-modifying complexes to regulate cell growth (2,4)."

reach
"We find that ASXL1 directs the binding of the positively charged C-terminal extension of BAP1 to nucleosomal DNA and histones H3-H4 near the dyad, an addition to its role in forming the ubiquitin-binding cleft."
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."
BAP1 deubiquitinates Histone.
| 3
BAP1 leads to the deubiquitination of Histone on A2. 3 / 3
| 3

reach
"p53 binds to the SLC7A11 promoter, directly suppressing its transcription; the nuclear deubiquitinase (DUB) BAP1 represses SLC7A11 transcription by removing histone 2A ubiquitination from the SLC7A11 promoter; and KEAP1 represses SLC7A11 transcription through degrading NRF2, a master transcription factor of antioxidant response and regulator of SLC7A11."

reach
"Further mechanistic studies demonstrated that BAP1 suppresses SLC7A11 expression partly by deubiquitinating histone 2A ubiquitination to promote ferroptosis."

reach
"Recently, it has been found that BAP1 can inhibit the expression of SLC7A11 to suppress the uptake of cystine by deubiquitinating histone 2A on the SLC7A11 promoter."
BAP1 activates Histone.
| 2
BAP1 activates Histone. 2 / 2
| 2

reach
"The BAP1 nuclear deubiquitinase is known to target histones (together with ASXL1 as a Polycomb repressor subunit) and the HCF1 transcriptional co-factor, and we show that BAP1 knockdown in MPM cell lines affects E2F and Polycomb target genes."

reach
"BAP1 is a deubiquitinase targeting histones and the host cell factor-1 transcriptional co-factor, and plays a role in transcriptional regulation, chromatin modulation, cell cycle regulation, and DNA repair."
BAP1 affects ASXL3
2 | 5 4
2 | 5 4

reach
"While ASXL1 and ASXL2 were known to interact with BAP1 from proteomics studies XREF_BIBR XREF_BIBR, ASXL3 was not detected as a BAP1 binding partner, possibly because of the low levels of BAP1 and ASXL3 complexes in cells."

No evidence text available

No evidence text available

reach
"ASXL3 interacts with BAP1, a ubiquitin terminal hydroxylase and removes the mono-ubiquitin (Ub1) from the H2AK119Ub1 [XREF_BIBR]."

reach
"It suggests that there may be a functional association between BAP1 and ASXL3 in cells and further cell based work is required to shed more light on this issue."

reach
"We found that ASXL3 interacts with BAP1, a hydrolase that removes mono-ubiquitin from histone H2A lysine 119 (H2AK119Ub1) as a component of the Polycomb repressive deubiquitination (PR-DUB) complex."

sparser
"We found that ASXL3 interacts with BAP1, a hydrolase that removes mono-ubiquitin from histone H2A lysine 119 (H2AK119Ub1) as a component of the Polycomb repressive deubiquitination (PR-DUB) complex."

sparser
"Interestingly, the truncated ASXL3 protein interacts with BAP1 protein ( xref )."

sparser
"ASXL3 interacts with BAP1, a ubiquitin terminal hydroxylase and removes the mono-ubiquitin (Ub1) from the H2AK119Ub1 [ xref ]."

sparser
"Our in vitro analysis indicates that also ASXL3 can bind and activate BAP1, which is not surprising in the light of the high sequence similarity between the DEUBAD domains in the ASXL family."
SETD2 affects PBRM1
| 11
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
SETD2 binds PBRM1 and BAP1. 3 / 3
| 3

sparser
"The mutations in the chromatin regulators PBRM1 , BAP1 , and SETD2 were differentially associated with altered expression patterns of large numbers of genes when compared to samples bearing a background of VHL mutation ( xref , xref )."

sparser
"In fact this association with chromosome 3p loss has recently been strengthened by several publications demonstrating that 4 out of five ( VHL , PBRM1 , SETD2 and BAP1 ) of the most frequently mutated genes associated with CCRCC are on chromosome 3p xref – xref and that the mutation of either SETD2 , BAP1 or PBRM1 associates with poorer prognosis or progression in those patients xref , xref , xref ."

sparser
"On univariable analysis SETD2 (HR: 3.30), BAP1 (HR: 2.44) and PBRM1 (HR: 1.78) were significantly associated with a higher risk of metastases."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
MP46 affects BAP1
| 11
BAP1 binds MP46. 10 / 11
| 11

sparser
"Following PAL treatment, OCR levels increased significantly in MP46, but were unchanged in MP46-BAP1 ( xref ), suggesting that MP46 utilized or metabolized FA more efficiently than MP46-BAP1."

sparser
"Of note, BAP1 expression was increased in hypoxic conditions in MP46-BAP1 ( xref ), implying the association between hypoxic-induced HIF1α and exogenous BAP1 expression."

sparser
"We did not observe significant differences in glycolysis, glycolytic capacity or OXPHOS parameters between MP46 and MP46-BAP1 ( xref ), although SRC levels were lower in MP46 than MP46-BAP1 ( xref )."

sparser
"We then compared FAO-oxygen consumption rate (OCR) levels between MP46 and MP46-BAP1 by Seahorse analysis."

sparser
"As expected, neither WZB117 nor 6-AN had any significant effect on the MP46 and MP46-BAP1 growth ( xref )."

sparser
"We further investigated the metabolite levels related to FA metabolism and observed MP46 had significantly lower levels of FAs and palmitoylcarnitine, which is involved in FA transport into mitochondria for oxidation compared to MP46-BAP1, but higher acetyl-carnitine levels, a byproduct of FAO ( xref )."

sparser
"In comparison to MP46-BAP1, the predominant (56%) metabolites increased in MP46 were related to nucleotide biosynthesis pathways, while most metabolite decreases were associated with amino acid and FA metabolism ( xref and xref )."

sparser
"First, we measured FA utilization levels between MP46 and MP46-BAP1 by incubating cells with palmitate (PAL) as the only oxidizable energy substrate."

sparser
"Next, we treated MP46 and MP46-BAP1 with IACS or ETO."

sparser
"We also tested for FAO-dependent OCR between MP46 and MP46-BAP1 by treatment with the FAO inhibitor, perhexiline (PHX)."
BAP1 affects Mesothelioma
| 11
BAP1 inhibits Mesothelioma.
| 6
| 6

reach
"Loss of BAP1 was shown to increase enhancer of zeste homolog 2 (EZH2) levels in a mesothelioma model , and to increase sensitivity to EZH2 inhibition."

reach
"We investigated whether loss of BAP1 expression can be used to support a diagnosis of mesothelioma in effusion cytology."

reach
"BAP1 loss induces mitotic defects in mesothelioma cells through BRCA1-dependent and independent mechanisms."

reach
"While the potential link between BAP1 loss—a known driver of mesothelioma—with response to ICI treatments is intriguing, an important caveat is that BAP1 resides at 3p21.1, a chromosomal region that also contains two other genes encoding epigenetic regulators, PBRM1 and SETD2."

reach
"EZH2 inhibitor tazemetostat in patients with relapsed or refractory, BAP1-inactivated malignant pleural mesothelioma: a multicentre, open-label, phase 2 study."

reach
"Germline mutations in BAP1 predispose carriers to the BAP1 tumor predisposition syndrome (BAP1-TPDS), characterized by BAP1-inactivated nevi (BINs), uveal melanoma, cutaneous melanoma, mesothelioma, renal cell carcinoma, and other tumors."
BAP1 activates Mesothelioma.
| 5
| 5

reach
"The authors demonstrated a possible role of the polycomb repressive complex 2 (PRC2) in Bap1-mediated mesothelioma."

reach
"Interestingly, it has been shown that germline mutations in Bap1 can induce epigenetic deregulation of the Rb protein in mice, facilitating the development of malignant mesothelioma [72]."

reach
"BAP1 PGVs accelerate mesothelioma tumorigenesis after asbestos exposure in preclinical models and may be partly predicted by clinical criteria."

reach
"Germline mutations in BAP1 greatly enhanced the sensitivity of mice to develop mesothelioma when exposed to asbestos 50."

reach
"In this regard, mesothelioma is considered a malignancy that cannot be cured surgically; however, there are a few cases of patients from families with malignant mesothelioma caused by germline BAP1 or other tumor suppressor mutations who, because of screening and a high degree of suspicion, underwent surgery at a very early stage, and most of them are alive and apparently tumor-free 10 years postsurgical resection (2 of them at 18 years and 21 years, respectively, postsurgery)."
BAP1 affects MP46
| 11
BAP1 binds MP46. 10 / 11
| 11

sparser
"Following PAL treatment, OCR levels increased significantly in MP46, but were unchanged in MP46-BAP1 ( xref ), suggesting that MP46 utilized or metabolized FA more efficiently than MP46-BAP1."

sparser
"Of note, BAP1 expression was increased in hypoxic conditions in MP46-BAP1 ( xref ), implying the association between hypoxic-induced HIF1α and exogenous BAP1 expression."

sparser
"We did not observe significant differences in glycolysis, glycolytic capacity or OXPHOS parameters between MP46 and MP46-BAP1 ( xref ), although SRC levels were lower in MP46 than MP46-BAP1 ( xref )."

sparser
"We then compared FAO-oxygen consumption rate (OCR) levels between MP46 and MP46-BAP1 by Seahorse analysis."

sparser
"As expected, neither WZB117 nor 6-AN had any significant effect on the MP46 and MP46-BAP1 growth ( xref )."

sparser
"We further investigated the metabolite levels related to FA metabolism and observed MP46 had significantly lower levels of FAs and palmitoylcarnitine, which is involved in FA transport into mitochondria for oxidation compared to MP46-BAP1, but higher acetyl-carnitine levels, a byproduct of FAO ( xref )."

sparser
"In comparison to MP46-BAP1, the predominant (56%) metabolites increased in MP46 were related to nucleotide biosynthesis pathways, while most metabolite decreases were associated with amino acid and FA metabolism ( xref and xref )."

sparser
"First, we measured FA utilization levels between MP46 and MP46-BAP1 by incubating cells with palmitate (PAL) as the only oxidizable energy substrate."

sparser
"Next, we treated MP46 and MP46-BAP1 with IACS or ETO."

sparser
"We also tested for FAO-dependent OCR between MP46 and MP46-BAP1 by treatment with the FAO inhibitor, perhexiline (PHX)."
VHL affects BAP1
| 3 7
| 3 3

reach
"The association between VHL, PBRM1, BAP1, SETD2 AND KDM5C mutation status and overall survival was evaluated in 167 patients with metastatic ccRCC who underwent genomic sequencing of their primary tumor."

sparser
"In keeping with their previous work demonstrating that BAP1 loss results in mTOR activation, the authors found that Vhl-Bap1 –deficient tumors showed strong phospho-S6 (pS6) expression."

reach
"Mice deficient for either VHL or VHL together with one allele of BAP1 developed multiple lesions spanning from benign cysts to cystic and solid ccRCC XREF_BIBR suggesting a tumor suppressive cooperation between BAP1 and pVHL."

sparser
"The faithful Vhl-Bap1 and Vhl-Pbrm1 GEM models developed by Gu and colleagues add to the recent salvo of GEM models of ccRCC based upon concurrent deletion of Vhl, p53 , and Rb1 by Frew and colleagues or the inactivation of Vhl and Cdkn2a with concurrent activation of MYC by our group ( xref , xref )."

reach
"All three genes are located on chromosome 3p, as is VHL and as noted earlier, inactivation of both VHL and Bap1 in mice leads to both cystic and neoplastic lesions [86]; clearly, definition of the functional interactions between VHL, BAP1, PBRM1, and SET is a topic of urgent interest."

sparser
"A significantly increased expression of HIF1a, and a decreased expression of VHL were associated with monosomy 3/loss of BAP1 expression."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
ITPR3 affects BAP1
| 5 4
ITPR3 binds BAP1.
| 3 4
| 2 4

sparser
"Then BAP1 binds and deubiquitinates IP3R3 in the ER, leading to Calcium release into the cytosol and mitochondria, and initiation of apoptotic pathways."

reach
"The data clearly indicated that BAP1 physically interacts with the type-3 inositol-1,4,5-trisphosphate-receptor (IP3R3), and BAP1 expression level is directly correlated with IP3R3 in wild-type and mutant fibroblasts."

sparser
"We confirmed the specificity of the BAP1 interaction with IP3R3 in HEK-293 cells expressing FLAG-BAP1 ( xref )."

sparser
"Recently, a novel BAP1 cytoplasmic function was introduced, showing that ER-localized BAP1 can bind and stabilize IP3R3 to modulate Ca2+ release resulting in apoptosis [ xref ]."

sparser
"We found that BAP1 binds the N-terminus domain of IP3R3 [IP3R3(NT)] ( xref )."

reach
"Overexpression of the N-terminus of the IP3R3 binds and sequesters BAP1 leading to decreased IP3R3 levels, an effect counter balanced by BAP1 co-overexpression (XREF_FIG)."
ITPR3 binds BAP1-C91S. 1 / 2
| 1

reach
"Instead, overexpression of the Myc tagged truncated BAP1 L-mutant [Myc-BAP1 (L)] (the L family mutant BAP1 which lacks the N-terminus domain 5) showed an almost complete loss of interaction with IP3R3 compared to Myc tagged WT BAP1 (Myc-BAP1) (XREF_FIG), while catalytic inactive BAP1 (C91S) XREF_BIBR, XREF_BIBR did bind IP3R3 (XREF_FIG)."
ITPR3 activates BAP1.
| 2
ITPR3 activates BAP1. 2 / 2
| 2

reach
"Reduced levels of BAP1 in BAP1 +/- carriers cause reduction both of IP3R3 levels and of Ca 2+ flux, preventing BAP1 +/- cells that accumulate DNA damage from executing apoptosis."

reach
"Reduced levels of BAP1 in BAP1 +/- carriers caused reduction of both IP3R3 levels and Ca 2+ flux, preventing BAP1 +/- cells that had accumulated DNA damage from executing apoptosis."
FlaG affects BAP1
| 10
| 10

sparser
"In order to identify the domains of BAP1 that interact with KEAP1, we generated FLAG-BAP1 mutant plasmids lacking individual domains (FLAG-BAP1ΔCD, FLAG-BAP1ΔCC1, FLAG-BAP1ΔCL, FLAG-BAP1ΔCC2, FLAG-BAP1ΔULD, and FLAG-BAP1ΔCTE)."

sparser
"Our result shows that FLAG-BAP1 does not selectively bind to HA-KEAP1ΔBTB ( xref A, right panel), suggesting that BAP1 binds to the BTB domain of KEAP1."

sparser
"Our results show that transduced FLAG-BAP1 is mostly located in the cytosol ( xref A)."

sparser
"In addition, lentiviral transduction of FLAG-BAP1 suppressed the migration ( xref E) and the invasion ( xref F) of A549 cells, and potentiated cisplatin-induced apoptosis in A549 cells ( xref G,H)."

sparser
"Similar transverse distributions of each protein were observed in biofilms formed by a Δbap1 mutant expressing Bap1-FLAG from a plasmid alone and by a ΔrbmA mutant expressing RbmA-FLAG from a plasmid alone (data not shown)."

sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."

sparser
"However, FLAG-BAP1 failed to attenuate a decrease in the level of HA-KEAP1ΔBTB induced by treatment of CHX in 293T-HA-KEAP1ΔBTB cells ( xref C, right panel), suggesting that the BTB domain is responsible for maintaining the protein stability of KEAP1 by BAP1."

sparser
"Real-time RT-PCR analysis demonstrates that lentiviral transduction of FLAG-BAP1 significantly decreased the mRNA level of NRF2 target genes such as heme oxygenase-1 (Hmox1), NADPH:quinone oxidoreductase-1 (Nqo1), glutamate cysteine ligase catalytic subunit (Gclc), and aldo-keto reductase family 1 member B10 (Akr1b10) in A549 cells ( xref D)."

sparser
"However, FLAG-BAP1 did not bind to HA-NRF2 ( xref C)."

sparser
"Our results show that treatment of CHX decreased the level of HA-KEAP1, and this event was significantly attenuated by overexpression of FLAG-BAP1 in 293T-HA-KEAP1 cells ( xref C, left panel)."

reach
"Phosphorylation of BAP1 that promotes HR resulted in reduced chromatin binding of BAP1 ."

reach
"BAP1 deubiquitylates Ub-H2AK119 at sites of DNA damage, and the loss of BAP1 has been shown to impair homologous recombination (HR) repair (10)."

reach
"The function of UCHL1 in DSB repair is still unclear, but it has been established that UCHL5 promotes repair by enhancing DNA-end resection, whereas BAP1 promotes HR via an unknown mechanism ."

reach
"This suggests that deubiquitination of H2A by BAP1 promotes HR which is complementary to the finding that ubiquitination of K119 in response to DNA damage favors NHEJ [95]."

reach
"BAP1 loss impairs HR and double-strand break repair, promoting error-prone nonhomologous end joining, with consequent genomic instability (XREF_FIG)."

reach
"BAP1 deubiquitylates Ub-H2AK119 at sites of DNA damage, and the loss of BAP1 has been shown to impair homologous recombination (HR) repair (10)."

reach
"In the nucleus, BAP1 modulates the transcriptional regulation of several gene programs and promotes DNA repair by facilitating homologous recombination ."

reach
"BAP1 is rapidly recruited to DSB sites, and BAP1 depletion leads to reduced BRCA1, RAD51 and RPA foci and has been suggested to decrease HR."

reach
"BAP1 loss specifically reduces HR but not NHEJ [68,69]."

reach
"As BAP1 loss causes deficient HR, BAP1-mutant tumors were reported to be particularly addicted to alternative DNA repair pathways, e.g., PARP1 mediated ones."
BAP1 affects VHL
| 3 7
| 3 3

reach
"The association between VHL, PBRM1, BAP1, SETD2 AND KDM5C mutation status and overall survival was evaluated in 167 patients with metastatic ccRCC who underwent genomic sequencing of their primary tumor."

sparser
"In keeping with their previous work demonstrating that BAP1 loss results in mTOR activation, the authors found that Vhl-Bap1 –deficient tumors showed strong phospho-S6 (pS6) expression."

reach
"Mice deficient for either VHL or VHL together with one allele of BAP1 developed multiple lesions spanning from benign cysts to cystic and solid ccRCC XREF_BIBR suggesting a tumor suppressive cooperation between BAP1 and pVHL."

sparser
"The faithful Vhl-Bap1 and Vhl-Pbrm1 GEM models developed by Gu and colleagues add to the recent salvo of GEM models of ccRCC based upon concurrent deletion of Vhl, p53 , and Rb1 by Frew and colleagues or the inactivation of Vhl and Cdkn2a with concurrent activation of MYC by our group ( xref , xref )."

reach
"All three genes are located on chromosome 3p, as is VHL and as noted earlier, inactivation of both VHL and Bap1 in mice leads to both cystic and neoplastic lesions [86]; clearly, definition of the functional interactions between VHL, BAP1, PBRM1, and SET is a topic of urgent interest."

sparser
"A significantly increased expression of HIF1a, and a decreased expression of VHL were associated with monosomy 3/loss of BAP1 expression."
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
BAP1 affects Melanoma
| 10
BAP1 inhibits Melanoma.
| 8
| 8

reach
"BINs are highly penetrant, present in up to 90% of mutation carriers, and typically present as multiple, skin colored or reddish-brown, dome-shaped melanocytic tumors (also or previously called BAP1-inactivlated melanocytic tumors, Wiesner nevi, BAPomas, nevoid melanoma-like melanocytic proliferations, BAP1 mutant Spitz nevi, and melanocytic BAP1-mutated atypical intradermal tumors)."

reach
"Germline mutations in BAP1 predispose carriers to the BAP1 tumor predisposition syndrome (BAP1-TPDS), characterized by BAP1-inactivated nevi (BINs), uveal melanoma, cutaneous melanoma, mesothelioma, renal cell carcinoma, and other tumors."

reach
"To the best of our knowledge, this is the first case of BAP1-inactivated melanoma with a documented TERT-p hot spot mutation manifesting as the first presentation of BAP1 tumor predisposition syndrome."

reach
"Cutaneous BAP1-inactivated melanocytic tumors, previously classified as atypical Spitz Nevi, may have a dermal mitotic activity that can resemble melanoma and on the other hand, atypical Spitz tumors are sometimes difficult to differentiate from BAP1-inactivated melanoma."

reach
"The addition of BAP1 loss increased tumor proliferation and melanoma size."

reach
"BAP1 Loss Promotes Suppressive Tumor Immune Microenvironment via Upregulation of PROS1 in Class 2 Uveal Melanomas."

reach
"Harbour et al. do not demonstrate that BAP1 re-expression is sufficient to suppress the metastatic potential of uveal melanomas; however, if it does as one would predict from this study, substrates critical in migration, anoikis, and colonization should be considered."

reach
"BAP1-Inactivated Melanoma Arising From BAP1-Inactivated Melanocytic Tumor in a Patient With BAP1 Germline Mutation: A Case Report and Review of the Literature."
BAP1 activates Melanoma.
| 2
BAP1 activates Melanoma. 2 / 2
| 2

reach
"CDKN2A, CDK4, MCR1, BAP1, and TERT promoter germline mutations increase melanoma risk within families."

reach
"Melanoma predisposition syndromes caused by pathogenic variants in POT1 and BAP1 are more recently described, and both are associated with Spitzoid tumors."
BAP1 affects FlaG
| 10
| 10

sparser
"In order to identify the domains of BAP1 that interact with KEAP1, we generated FLAG-BAP1 mutant plasmids lacking individual domains (FLAG-BAP1ΔCD, FLAG-BAP1ΔCC1, FLAG-BAP1ΔCL, FLAG-BAP1ΔCC2, FLAG-BAP1ΔULD, and FLAG-BAP1ΔCTE)."

sparser
"Our result shows that FLAG-BAP1 does not selectively bind to HA-KEAP1ΔBTB ( xref A, right panel), suggesting that BAP1 binds to the BTB domain of KEAP1."

sparser
"Our results show that transduced FLAG-BAP1 is mostly located in the cytosol ( xref A)."

sparser
"In addition, lentiviral transduction of FLAG-BAP1 suppressed the migration ( xref E) and the invasion ( xref F) of A549 cells, and potentiated cisplatin-induced apoptosis in A549 cells ( xref G,H)."

sparser
"Similar transverse distributions of each protein were observed in biofilms formed by a Δbap1 mutant expressing Bap1-FLAG from a plasmid alone and by a ΔrbmA mutant expressing RbmA-FLAG from a plasmid alone (data not shown)."

sparser
"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."

sparser
"However, FLAG-BAP1 failed to attenuate a decrease in the level of HA-KEAP1ΔBTB induced by treatment of CHX in 293T-HA-KEAP1ΔBTB cells ( xref C, right panel), suggesting that the BTB domain is responsible for maintaining the protein stability of KEAP1 by BAP1."

sparser
"Real-time RT-PCR analysis demonstrates that lentiviral transduction of FLAG-BAP1 significantly decreased the mRNA level of NRF2 target genes such as heme oxygenase-1 (Hmox1), NADPH:quinone oxidoreductase-1 (Nqo1), glutamate cysteine ligase catalytic subunit (Gclc), and aldo-keto reductase family 1 member B10 (Akr1b10) in A549 cells ( xref D)."

sparser
"However, FLAG-BAP1 did not bind to HA-NRF2 ( xref C)."

sparser
"Our results show that treatment of CHX decreased the level of HA-KEAP1, and this event was significantly attenuated by overexpression of FLAG-BAP1 in 293T-HA-KEAP1 cells ( xref C, left panel)."
| 1 7
BAP1 inhibits Cell Survival.
| 1 3
| 1 3

reach
"In summary, it seems that loss of BAP1 foster genomic instability in tumor pathogenesis, however, the activity of BAP1 promotes tumor cell survival and contributes to therapeutic resistance during irradiation."

eidos
"We found that BaP1 is selective for CRC cells and reduces cell proliferation , cell survival , and cell migration ."

reach
"Crucially, concomitant BAP1 and HDAC2 depletion did not additively reduce cell viability in the presence of vorinostat."

reach
"BAP1 suppresses cell viability and growth."
BAP1 activates Cell Survival.
| 4
| 4

reach
"Our results suggested that, whereas WT BAP1 suppresses MM cell proliferation and restores cell survival after IR damage, some mutant BAP1 may also moderately retain these functions."

reach
"Further, downregulation of BAP1 in SK-N-FI cells by using small interfering RNA (siRNA) enhanced cell survival compared to siRNA treated control cells."

reach
"BAP1 promotes stalled fork restart and cell survival via INO80 in response to replication stress."

reach
"BAP1 stabilizes BRCA1 protein and restores cell survival rates of MM cells with BAP1 deletion after IR."
| 10
BAP1 activates Carcinogenesis.
| 8
| 5

reach
"BAP1 PGVs accelerate mesothelioma tumorigenesis after asbestos exposure in preclinical models and may be partly predicted by clinical criteria."

reach
"While RNF2, through H2A monoubiquitination, silences the BCL2 and MCL1 prosurvival genes and thereby induces apoptosis in some cell types, this does not occur in melanocytes, suggesting that BAP1 promotes tumorigenesis in cells that do not engage such RNF2 apoptotic programme.Moving to the p53 tumor suppressor, Karen Vousden (London, UK), who was awarded the CDD Award 2018, retraced the long road leading to her key discoveries of p53 functions."

reach
"Besides, a study of families with BAP1 germline mutations shows that mutated BAP1 may promote oncogenesis and increase the risk of KIRC by 8-fold through altering cellular metabolism and the tumor microenvironment (25)."

reach
"For instance, in the ~40% of tumors that are seemingly wild-type for both PBRM1 and BAP1, what other events cooperate with VHL loss to promote oncogenesis?"

reach
"Among them, BAP1 and BLM are present as a germline inactivation in a small subset of patients and increases predisposition to tumorigenesis."
Mutated BAP1 activates Carcinogenesis. 3 / 3
| 3

reach
"There has been increased interest in the mechanisms by which BAP1 mutations lead to carcinogenesis."

reach
"We aim to give a concise review of the carcinogenesis caused by BAP1 mutations, and discuss clinical implications including suggested screening guidelines for patients with germline BAP1 mutations, and potential targeted therapeutic options in patients with mutated BAP1."

reach
"With respect to cancer biology, BAP1 loss confers metabolic advantages to cancer cells that might partially account for how BAP1 mutations increase the susceptibility of tumorigenesis (62,65)."
BAP1 inhibits Carcinogenesis.
| 2

reach
"A subsequent study reported that BAP1 modulated gene expression in a cell-type specific manner, and loss of BAP1 promoted tumorigenesis in cells that did not engage an RNF2-dependent apoptotic program ."

reach
"Loss of one BAP1 allele either inherited or acquired during life has been associated with environmental stress-induced carcinogenesis, like UV light for uveal melanoma and asbestos for mesothelioma."
PGR affects BAP1
| 9
| 6

sparser
"Our results reveal that the physiological modulation of BAP1 determines the invasive properties of the trophoblast, delineating a new role of the BAP1 PR-DUB complex in regulating early placentation."

sparser
"Together, our data suggested a model that the BAP1-containing PR-DUB complex binds on the S LC7A11 promoter, where BAP1 removes ubiquitin from H2Aub, and BAP1-dependent H2Aub reduction on SLC7A11 is associated with BAP1-mediated SLC7A11 repression."

sparser
"These BAP1 mutants did not significantly affect BAP1’s interaction with other components of the PR-DUB complex ( xref )."

sparser
"Polycomb group complexes, including the BAP1 PR-DUB, are well conserved throughout evolution ( xref ), leading to the question whether BAP1 also functions to regulate trophoblast differentiation and invasion during human placentation."

sparser
"BAP1 PR-DUB complex is also regulated during human trophoblast differentiation."

sparser
"In human cells, ASXL3 protein was shown to interact with BAP1 to form the PR-DUB complex ( xref )."
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
FOXK2 binds PGR and BAP1. 1 / 1
| 1

sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
OGT affects BAP1
4 | 3 1
4 | 1 1

No evidence text available

No evidence text available

reach
"Knock-in mice expressing BAP1 with a 3xFlag tag revealed that BAP1 interacts with HCF-1, OGT, and the polycomb group proteins ASXL1 and ASXL2 in vivo."

No evidence text available

sparser
"BAP1 interacts with OGT in the PR-DUB complex, and our data suggest that the PR-DUB complex plays a multifunctional role in repression of the injury program."

No evidence text available
HCFC1 binds OGT and BAP1. 2 / 2
| 2

reach
"The fact that HCF-1 acts as an important modulator of cellular circadian rhythm [XREF_BIBR] raises the possibility that the OGT, HCF-1, and BAP1 complex interacts with the BMAL1, CLOCK, and PER complex to modulate circadian rhythm."

reach
"Within the BAP1/HCF-1/OGT complex, HCF1 is modified by O-GlcNacylation, the addition of single O-linked N-acetylglucosamine moiety to specific serine or threonine residues (50,51)."
FOXK1 affects BAP1
7 | 1 1
7 | 1 1

No evidence text available

sparser
"For example, analysis of 407 pleural mesothelioma cases and 389 controls with a comprehensive history of asbestos exposure revealed an increased risk of abnormalities in chromosomal region 7p22.2, which includes the gene encoding the Forkhead box protein K1 (FOXK1) that interacts with BAP1 [ xref ]."
| PMC

No evidence text available

reach
"Although BAP1 interacts with a transcriptional regulator, HCF-1, and transcription factors FoxK1 and FoxK2, how BAP1 controls gene expression remains unclear."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
EED affects BAP1
| 9
| 5

sparser
"The interaction with BRCA1 and BARD1 form a tumor suppressor heterodimeric complex. [ xref ] BAP1 regulates cell proliferation and interacts with histone-modifying complexes during cell division. [ xref , xref ] BAP1 also forms the Polycomb group repressive deubiquitinase complex (PR-DUB) through interaction with ASXL1, which is involved several developmental processes. [ xref ] Because of this functional complexity, BAP1 germline mutations predispose individuals to the aggressive tumor phenotypes."

sparser
"BAP1 interacts with ASXL family members to form the polycomb group repressive deubiquitinase complex involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"Deubiquitination of nucleosomal H2A is performed using a minimal Polycomb Repressive Deubiquitinase (PR-DUB) complex formed by the human deubiquitinase BAP1 and the DEUBiquitinase ADaptor (DEUBAD) domain of its co-factor ASXL2."

sparser
"In this study, we have identified a critical link between Polycomb repressive deubiquitinase-BAP1 (PR-DUB) complex and BRD4, which is bridged by the physical interaction between additional sex combs-like protein 3 (ASXL3) in SCLC."

sparser
"Here, we show how these mutations affect BAP1 interactions with the Polycomb group-like protein, ASXL2, using combinations of computational modeling technology, molecular biology, and in vitro reconstitution biochemistry."
ASXL1 binds EED and BAP1. 4 / 4
| 4

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

sparser
"BAP1 interacts with ASXL1 polycomb chromatin binding protein forming a polycomb repressive deubiquitinase complex that regulates histone H2A ubiquitination xref ."

sparser
"BAP1 also interacts with ASXL1 to form the Polycomb group repressive deubiquitinase complex (PR-DUB), which is involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"8 BAP1 interacts with ASXL1 (MIM 612990 ) to form the Polycomb group Repressive Deubiquitinase complex (PR-DUB)."
BAP1 affects ferroptosis
| 2 7
BAP1 activates ferroptosis.
| 2 4
| 2 4

reach
"These results are intriguing and suggest that, although BAP1 is a multimeric complex with several activities involved in its recruitment and control of its activity, some associated partners might impose another layer of regulation on BAP1-mediated ferroptosis."

eidos
"It was reported that the BRCA1-associated protein 1 ( BAP1 ) tumor suppressor is also able to promote ferroptosis by repressing SLC7A11 [ 41 ] ."

reach
"Unlike other tumour suppressors (e.g., p53, BAP1, KEAP1 and ARF (p14), which promote ferroptosis; see Box 2), this tumour-suppressive axis suppresses ferroptosis ."

eidos
"Studies have reported that BAP1 could promote ferroptosis by blocking the expression of SLC7A11 ."

reach
"It has been recently reported that BAP1 facilitates lipid peroxidation and promotes ferroptosis through repressing SLC7A11 [24]."

reach
"It was reported that the BRCA1-associated protein 1 (BAP1) tumor suppressor is also able to promote ferroptosis by repressing SLC7A11 [41]."
BAP1 inhibits ferroptosis.
| 3
| 3

reach
"Furthermore, the tumor suppressor BRCA1-associated protein 1 suppresses SLC7A11 transcription by decreasing H2Aub, leading to elevated lipid peroxidation and thus, increased ferroptosis (49)."

reach
"Zhang et al. showed that BAP1 inhibits tumor progression by promoting cellular ferroptosis (15)."

reach
"In addition, Zhang et al. discovered that another tumor suppressor BAP1 promoted ferroptosis and inhibited the growth of cancer cells by inhibiting SLC7A11 (49)."
BAP1 affects Syndrome
| 9
BAP1 activates Syndrome. 9 / 9
| 9

reach
"Furthermore, BAP1 germline mutations cause a tumor predisposition syndrome inherited in an autosomal dominant pattern, which confers a higher risk for developing a spectrum of benign and malignant tumors , including pancreatic cancer ."

reach
"Familial cancer predisposition syndrome caused by germline mutations in BAP1 is inherited in an autosomal dominant pattern."

reach
"The presence of multiple BAP1-negative melanocytic neoplasms is a hallmark of familial cancer susceptibility syndrome caused by germline mutations in BAP1."

reach
"Screening of genealogies from affected families led to the discovery of an inherited cancer syndrome caused by BAP1 germline mutations [68,69]."

reach
"Pathogenic germline variants in the BRCA1-associated protein 1 (BAP1) gene cause a dominantly inherited tumor predisposition syndrome, BAP1-TPDS, in which MM is the second most common associated cancer."

reach
"A rare familial syndrome at risk for ccRCC is caused by germline mutations in BAP1 ."

reach
"BIMTs can occur as a sporadic lesion or, less commonly, in the setting of an autosomal dominant cancer susceptibility syndrome caused by a BAP1 germline inactivating mutation."

reach
"Melanoma predisposition syndromes caused by pathogenic variants in POT1 and BAP1 are more recently described, and both are associated with Spitzoid tumors."

reach
"Altogether, these results define a clinical syndrome caused by rare germline missense BAP1 variants that alter chromatin remodeling through abnormal histone ubiquitination and lead to transcriptional dysregulation of developmental genes."
BAP1 affects PTEN
1 | 3 5
BAP1 binds PTEN.
1 | 1 5
1 | 1 3

sparser
"These findings demonstrate that BAP1 is an important deubiquitinase of PTEN for its stability and the BAP1-PTEN signaling axis plays a crucial role in tumor suppression."

sparser
"BAP1 can physically bind to and deubiquitinate PTEN, which inhibits the ubiquitination-mediated degradation of PTEN and thus stabilizes PTEN protein."

reach
"BAP1 can physically bind to and deubiquitinate PTEN, which inhibits the ubiquitination mediated degradation of PTEN and thus stabilizes PTEN protein."

No evidence text available

sparser
"In analyses adjusted only for cohort, mutations in BAP1 (median: 8 vs 5.2 cm; p = 0.001) and PTEN (median: 8 vs 5.9 cm; p = 0.026) were significantly associated with larger median tumor diameter."
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
BAP1 deubiquitinates PTEN.
| 2
BAP1 deubiquitinates PTEN. 2 / 2
| 2

reach
"BAP1 can physically bind to and deubiquitinate PTEN, which inhibits the ubiquitination mediated degradation of PTEN and thus stabilizes PTEN protein."

reach
"However, another study suggests that BAP1 may deubiquitinate and stabilize PTEN, leading to Akt inhibition [133]."
BAP1 affects PGR
| 9
| 6

sparser
"Our results reveal that the physiological modulation of BAP1 determines the invasive properties of the trophoblast, delineating a new role of the BAP1 PR-DUB complex in regulating early placentation."

sparser
"Together, our data suggested a model that the BAP1-containing PR-DUB complex binds on the S LC7A11 promoter, where BAP1 removes ubiquitin from H2Aub, and BAP1-dependent H2Aub reduction on SLC7A11 is associated with BAP1-mediated SLC7A11 repression."

sparser
"These BAP1 mutants did not significantly affect BAP1’s interaction with other components of the PR-DUB complex ( xref )."

sparser
"Polycomb group complexes, including the BAP1 PR-DUB, are well conserved throughout evolution ( xref ), leading to the question whether BAP1 also functions to regulate trophoblast differentiation and invasion during human placentation."

sparser
"BAP1 PR-DUB complex is also regulated during human trophoblast differentiation."

sparser
"In human cells, ASXL3 protein was shown to interact with BAP1 to form the PR-DUB complex ( xref )."
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
FOXK2 binds PGR and BAP1. 1 / 1
| 1

sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
BAP1 affects FOXK1
7 | 1 1
7 | 1 1

No evidence text available

sparser
"For example, analysis of 407 pleural mesothelioma cases and 389 controls with a comprehensive history of asbestos exposure revealed an increased risk of abnormalities in chromosomal region 7p22.2, which includes the gene encoding the Forkhead box protein K1 (FOXK1) that interacts with BAP1 [ xref ]."
| PMC

No evidence text available

reach
"Although BAP1 interacts with a transcriptional regulator, HCF-1, and transcription factors FoxK1 and FoxK2, how BAP1 controls gene expression remains unclear."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
BAP1 affects EED
| 9
| 5

sparser
"The interaction with BRCA1 and BARD1 form a tumor suppressor heterodimeric complex. [ xref ] BAP1 regulates cell proliferation and interacts with histone-modifying complexes during cell division. [ xref , xref ] BAP1 also forms the Polycomb group repressive deubiquitinase complex (PR-DUB) through interaction with ASXL1, which is involved several developmental processes. [ xref ] Because of this functional complexity, BAP1 germline mutations predispose individuals to the aggressive tumor phenotypes."

sparser
"BAP1 interacts with ASXL family members to form the polycomb group repressive deubiquitinase complex involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"Deubiquitination of nucleosomal H2A is performed using a minimal Polycomb Repressive Deubiquitinase (PR-DUB) complex formed by the human deubiquitinase BAP1 and the DEUBiquitinase ADaptor (DEUBAD) domain of its co-factor ASXL2."

sparser
"In this study, we have identified a critical link between Polycomb repressive deubiquitinase-BAP1 (PR-DUB) complex and BRD4, which is bridged by the physical interaction between additional sex combs-like protein 3 (ASXL3) in SCLC."

sparser
"Here, we show how these mutations affect BAP1 interactions with the Polycomb group-like protein, ASXL2, using combinations of computational modeling technology, molecular biology, and in vitro reconstitution biochemistry."
ASXL1 binds EED and BAP1. 4 / 4
| 4

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

sparser
"BAP1 interacts with ASXL1 polycomb chromatin binding protein forming a polycomb repressive deubiquitinase complex that regulates histone H2A ubiquitination xref ."

sparser
"BAP1 also interacts with ASXL1 to form the Polycomb group repressive deubiquitinase complex (PR-DUB), which is involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"8 BAP1 interacts with ASXL1 (MIM 612990 ) to form the Polycomb group Repressive Deubiquitinase complex (PR-DUB)."
P14_3_3 affects BAP1
| 8
| 8

sparser
"The reciprocal IP using 14-3-3 antibodies also indicated the formation of the complex between BAP1 and 14-3-3 (Fig.  xref )."

sparser
"We could also confirm that the short fragment of BAP1-ULD domain and 14-3-3-alpha helices 7-9 regions facilitate interaction between these two proteins (Fig.  xref ), while the interaction between BAP1 and 14-3-3-σ inactive mutants were reduced compared to the full-length 14-3-3-σ (Fig.  xref )."

sparser
"In addition, fluorescence resonance energy transfer (FRET) assay demonstrated a direct interaction between BAP1 and 14-3-3 (Fig.  xref )."

sparser
"These results suggest that BAP1 interaction with 14-3-3 prevents cell survival signaling, which is essential for growth of the neuroblastoma cells (Fig.  xref )."

sparser
"The interaction between BAP1 and 14-3-3 protein was confirmed by performing the co-IP of FLAG-tagged BAP1 and pull-down of endogenous 14-3-3 in neuroblastoma cells (Fig.  xref )."

sparser
"Next, we tested the hypothesis that the BAP1 interaction with 14-3-3 releases the binding between 14-3-3 and Bax, which further facilitates cell death."

sparser
"BAP1 binding to 14-3-3 facilitate the apoptotic signaling in neuroblastoma."

sparser
"Another study in 2018, showed that BAP1 interacts with the protein 14-3-3 in neuroblastoma cells causing the release of Bax and the decrease of pro-survival factors such as Bcl-2, to promote apoptosis via the intrinsic pathway.[ xref ]"
OmpT affects BAP1
| 3 5
| 3 5

sparser
"In presence of PmB, Bap1 was not able to bind to the OmpT protein from the Δ ompT /pBR- ompT * (LDV mutant) mutant to the same extent as to the wild type OmpT protein ( xref ), suggesting a role for the LDV domain in Bap1 binding to OmpT on OMVs ( xref )."

sparser
"Bap1-OmpT interaction requires the presence of the integrin-binding domain (leucine-aspartic acid-valine LDV peptide) of OmpT and the FG-GAP domains of Bap1 ( xref )."

reach
"Taken together, our results strongly support the hypothesis that Bap1 binds to OmpT on OMVs of V. cholerae A1552 through the LDV tripeptide integrin binding domain of OmpT."

reach
"We investigated Bap1 binding to OmpT, a major outer membrane porin of V. cholerae containing an integrin binding domain (LDV peptide)."

sparser
"We investigated Bap1 binding to OmpT, a major outer membrane porin of V. cholerae containing an integrin-binding domain (LDV peptide)."

sparser
"Taken together, our results strongly support the hypothesis that Bap1 binds to OmpT on OMVs of V. cholerae A1552 through the LDV tripeptide integrin-binding domain of OmpT."

sparser
"It was also reported that RbmC does not interact with OmpT in the OMVs, although it is currently unclear why Bap1, but not RbmC, binds to OmpT."

reach
"It has been reported that Bap1 could associate with outer membrane vesicles (OMVs) by binding to OmpT."
TMED2 affects BAP1
| 8
| 8

sparser
"Although the absolute and relative amounts of COPI that interacts with BAP1 in the AP-MS is low ( xref and xref ), the interaction can still be biologically relevant and is validated with endogenous protein as seen in the immunoblot experiments ( xref )."
| PMC

sparser
"Validation of the BAP1 COPI interaction."
| PMC

sparser
"To exclude that the BAP1 interaction with the COPI complex is an artifact of overexpression of the exogenous BAP1 construct, we immunoprecipitated endogenous BAP1 using different antibodies against BAP1 in cell lysate from HeLa FRT parental cells ( xref )."
| PMC

sparser
"We agree with reviewer 2 that the interaction between BAP1 and the COPI complex may be a substrate interaction as evidenced by the low protein stoichiometry observed in the affinity purification (Fig 5)."
| PMC

sparser
"We must be absolutely sure that the BAP1-COPI interaction is not an artifact."
| PMC

sparser
"AP-immunoblotting of GFP-COPE shows interaction of COPA as expected, however BAP1 cannot be observed which is probably due to the low levels of BAP1 that bind to the COPI complex ( xref )."
| PMC

sparser
"In order to confirm the interaction between BAP1 and the heptameric COPI complex we performed AP-immunoblot experiments ( xref )."
| PMC

sparser
"It was intriguing that BAP1 has such a sequence in its C-terminus, but our mutational analysis suggests that the interaction between BAP1 and the COPI complex is not mediated through the C-terminal KxKxx sequence in BAP1."
| PMC
MBD6 affects MBD5
| 8
MBD5 binds MBD6 and BAP1. 8 / 8
| 8

sparser
"This result indicates an indirect interaction between MBD5, MBD6, and BAP1."

sparser
"After testing the specificity of the antibodies, we performed immunoprecipitation in HEK293T cells and demonstrated a stable protein-protein interaction between endogenous MBD5, MBD6, and BAP1 (Fig. xref C, D)."

sparser
"Therefore, to determine whether ASXLs play a role in mediating the interaction between MBD5, MBD6, and BAP1, we knocked out all three ASXL genes (TKO) by CRISPR in HEK293T cells."

sparser
"Furthermore, we have demonstrated that MBD5 or MBD6 do not directly interact with BAP1; instead, they bind to the C-terminal PHD fingers of all three ASXL subunits (ASXL1-3)."

sparser
"Therefore, MBD5 and MBD6 have been excluded from the BAP1 complex due to a lack of evidence on endogenous protein-protein interaction between MBD5, MBD6, and BAP1 [ xref , xref – xref ]."

sparser
"To determine whether MBD5 and MBD6 can also bind to chromatin similar to other BAP1 subunits, we conducted Chromatin Immunopreciptiation Sequencing (ChIP-seq) and determined the chromatin binding profiles of MBD5 and MBD6 with our validated homemade antibodies (Additional file xref : Fig. S1F)."

sparser
"To investigate how MBD5 and MBD6 subunits are incorporated into the BAP1 complex, we first hypothesized that MBD5 and MBD6 may directly interact with BAP1 itself, similar to how other subunits function (e.g., ASXL1-3 and FOXK1/2)."

sparser
"Surprisingly, as shown in Fig. xref B, the protein-protein interaction between MBD5, MBD6, and BAP1 is completely abolished in ASXL-TKO cells (Fig. xref B)."
KDM5C affects BAP1
| 8
| 3

sparser
"Mutations of KDM5C and BAP1 were significantly associated with evidence of renal vein invasion (P = ."

sparser
"Mutations of the KDM5C and BAP1 genes were significantly associated with evidence of renal vein invasion on contrast-enhanced CT of ccRCC (p=0.022 and 0.046, respectively) The genotype of solid ccRCC differed significantly from the one of multicystic ccRCC."

sparser
"Mutations of KDM5C and BAP1 were significantly associated with evidence of renal vein invasion (p=0.022 and 0.046, respectively)."
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
BAP1 affects p14_3_3
| 8
| 8

sparser
"The reciprocal IP using 14-3-3 antibodies also indicated the formation of the complex between BAP1 and 14-3-3 (Fig.  xref )."

sparser
"We could also confirm that the short fragment of BAP1-ULD domain and 14-3-3-alpha helices 7-9 regions facilitate interaction between these two proteins (Fig.  xref ), while the interaction between BAP1 and 14-3-3-σ inactive mutants were reduced compared to the full-length 14-3-3-σ (Fig.  xref )."

sparser
"In addition, fluorescence resonance energy transfer (FRET) assay demonstrated a direct interaction between BAP1 and 14-3-3 (Fig.  xref )."

sparser
"These results suggest that BAP1 interaction with 14-3-3 prevents cell survival signaling, which is essential for growth of the neuroblastoma cells (Fig.  xref )."

sparser
"The interaction between BAP1 and 14-3-3 protein was confirmed by performing the co-IP of FLAG-tagged BAP1 and pull-down of endogenous 14-3-3 in neuroblastoma cells (Fig.  xref )."

sparser
"Next, we tested the hypothesis that the BAP1 interaction with 14-3-3 releases the binding between 14-3-3 and Bax, which further facilitates cell death."

sparser
"BAP1 binding to 14-3-3 facilitate the apoptotic signaling in neuroblastoma."

sparser
"Another study in 2018, showed that BAP1 interacts with the protein 14-3-3 in neuroblastoma cells causing the release of Bax and the decrease of pro-survival factors such as Bcl-2, to promote apoptosis via the intrinsic pathway.[ xref ]"
BAP1 affects ompT
| 3 5
| 3 5

sparser
"In presence of PmB, Bap1 was not able to bind to the OmpT protein from the Δ ompT /pBR- ompT * (LDV mutant) mutant to the same extent as to the wild type OmpT protein ( xref ), suggesting a role for the LDV domain in Bap1 binding to OmpT on OMVs ( xref )."

sparser
"Bap1-OmpT interaction requires the presence of the integrin-binding domain (leucine-aspartic acid-valine LDV peptide) of OmpT and the FG-GAP domains of Bap1 ( xref )."

reach
"Taken together, our results strongly support the hypothesis that Bap1 binds to OmpT on OMVs of V. cholerae A1552 through the LDV tripeptide integrin binding domain of OmpT."

reach
"We investigated Bap1 binding to OmpT, a major outer membrane porin of V. cholerae containing an integrin binding domain (LDV peptide)."

sparser
"We investigated Bap1 binding to OmpT, a major outer membrane porin of V. cholerae containing an integrin-binding domain (LDV peptide)."

sparser
"Taken together, our results strongly support the hypothesis that Bap1 binds to OmpT on OMVs of V. cholerae A1552 through the LDV tripeptide integrin-binding domain of OmpT."

sparser
"It was also reported that RbmC does not interact with OmpT in the OMVs, although it is currently unclear why Bap1, but not RbmC, binds to OmpT."

reach
"It has been reported that Bap1 could associate with outer membrane vesicles (OMVs) by binding to OmpT."

reach
"These results indicated that P-I metalloproteinases and LAAO induced both necrosis and late apoptotic mode of cells death (Fig. 3A, Additional file 3B)."

reach
"In functional assays, BAP1 was found to inhibit the migration and invasion of LAC cells, and promoted their apoptosis and necrosis."

reach
"P-I metalloproteinases and LAAO arrested the cells in G0/G1 phase of cell cycle and also led to cytotoxic activity in endothelial cells by inducing necrosis/late apoptotic mode of cell death."

reach
"Functionally, BAP1 promotes apoptosis and necrosis, and down-regulates the migration and invasion abilities of LAC cells."

reach
"It is also prudent to note that expression of BAP1 increased the population of cells that stain positive for both AnnexinV and PI, an indication of late apoptosis and/or necrosis."

reach
"In functional experiments, BAP1 has been shown to suppress lung cancer tumorigenesis in athymic nude mice, promote the cell cycle and induce both apoptosis and necrosis."
BAP1 affects miR-31
| 8
BAP1 inhibits miR-31.
| 3
BAP1 inhibits miR-31. 3 / 3
| 3

reach
"Additionally, BAP1 overexpression attenuated the promotive effect of miR-31 on tumor growth, suggesting that miR-31 might promote tumor growth by silencing BAP1."

reach
"Xenografts with both miR-31 and BAP1 overexpression exhibited reduced cell mitosis compared to xenografts with miR-31 overexpression, suggesting that BAP1 overexpression could attenuate the pro proliferative effect of miR-31."

reach
"Finally, the cells transfected with miR-31 and the BAP1 overexpression plasmid exhibited significantly lower proliferation rates compared with the cells transfected with miR-31 and the control plasmid, suggesting that miR-31-resistant BAP1 could rescue the suppression of BAP1 by miR-31 and attenuate the proliferative effect of miR-31."
BAP1 activates miR-31.
| 3
BAP1 activates miR-31. 3 / 3
| 3

reach
"In this study, we showed that BAP1 was a direct target of miR-31 and an inverse correlation between miR-31 and BAP1 expression in cervical cancer cells and tissues."

reach
"Bioinformatic and dual-luciferase reporter gene assay showed that BAP1 was the direct target of miR-31."

reach
"We further examined whether BAP1 was a direct target of miR-31 in cervical cancer cells using dual-luciferase reporter assay."
BAP1 binds miR-31.
| 2
BAP1 binds miR-31. 2 / 2
| 2

reach
"These results indicate that miR-31 binds to BAP1 3 '-UTR directly and negatively regulates BAP1 expression in cervical cancer cells."

reach
"The aim of this study was to evaluate the interaction between BAP1 and miR-31 in MPM and their prognostic role in MPM."
BAP1 affects TMED2
| 8
| 8

sparser
"Although the absolute and relative amounts of COPI that interacts with BAP1 in the AP-MS is low ( xref and xref ), the interaction can still be biologically relevant and is validated with endogenous protein as seen in the immunoblot experiments ( xref )."
| PMC

sparser
"Validation of the BAP1 COPI interaction."
| PMC

sparser
"To exclude that the BAP1 interaction with the COPI complex is an artifact of overexpression of the exogenous BAP1 construct, we immunoprecipitated endogenous BAP1 using different antibodies against BAP1 in cell lysate from HeLa FRT parental cells ( xref )."
| PMC

sparser
"We agree with reviewer 2 that the interaction between BAP1 and the COPI complex may be a substrate interaction as evidenced by the low protein stoichiometry observed in the affinity purification (Fig 5)."
| PMC

sparser
"We must be absolutely sure that the BAP1-COPI interaction is not an artifact."
| PMC

sparser
"AP-immunoblotting of GFP-COPE shows interaction of COPA as expected, however BAP1 cannot be observed which is probably due to the low levels of BAP1 that bind to the COPI complex ( xref )."
| PMC

sparser
"In order to confirm the interaction between BAP1 and the heptameric COPI complex we performed AP-immunoblot experiments ( xref )."
| PMC

sparser
"It was intriguing that BAP1 has such a sequence in its C-terminus, but our mutational analysis suggests that the interaction between BAP1 and the COPI complex is not mediated through the C-terminal KxKxx sequence in BAP1."
| PMC
BAP1 affects PTGS2
| 8
BAP1 increases the amount of PTGS2. 8 / 8
| 8

reach
"Accordingly, our data show that BaP1 induced a marked increase in protein expression of both COX-2 and mPGES-1, the increase in COX-2 expression occurring before that of mPGES-1."

reach
"We found that the EP4 receptor positively regulates BaP1-triggered PGE release and expression of COX-2 and mPGES-1, which are critically involved in the biosynthesis of PGE induced by this metalloprotease."

reach
"Furthermore, our findings that BaP1 induced gene expression of COX-2 and mPGES-1 demonstrate that BaP1 can trigger transcriptional mechanisms in FLSs that result in increased protein expression."

reach
"Expression of COX-2 and mPGES-1 are induced by BaP1 via activation of NF-kappaB pathway."

reach
"Having shown that the inducible COX-2 isoform participates in the signaling pathway triggered by BaP1 that leads to PGE production, we next investigated whether BaP1 is able to induce protein expression of COX-2 in FLSs."

reach
"In view of previous reports that IL-1β induces PGE production via up-regulation of COX-2 and PGESm-1 expression in several cell types, including synovial fibroblasts , it is plausible to suggest that, under the present experimental conditions, IL-1β up-regulates BaP1-stimulated production of PGE by inducing expression of COX-2 and/or mPGES-1 in FLSs."

reach
"Expression of COX-2 and mPGES-1 are induced by BaP1 via activation of NF-kappaB pathway."

reach
"Gene and protein expression of both COX-2 and mPGES-1 are induced by BaP1 via activation of distinct subunits of the NF-κB transcription factor."
BAP1 affects MBD6
| 8
MBD5 binds MBD6 and BAP1. 8 / 8
| 8

sparser
"This result indicates an indirect interaction between MBD5, MBD6, and BAP1."

sparser
"After testing the specificity of the antibodies, we performed immunoprecipitation in HEK293T cells and demonstrated a stable protein-protein interaction between endogenous MBD5, MBD6, and BAP1 (Fig. xref C, D)."

sparser
"Therefore, to determine whether ASXLs play a role in mediating the interaction between MBD5, MBD6, and BAP1, we knocked out all three ASXL genes (TKO) by CRISPR in HEK293T cells."

sparser
"Furthermore, we have demonstrated that MBD5 or MBD6 do not directly interact with BAP1; instead, they bind to the C-terminal PHD fingers of all three ASXL subunits (ASXL1-3)."

sparser
"Therefore, MBD5 and MBD6 have been excluded from the BAP1 complex due to a lack of evidence on endogenous protein-protein interaction between MBD5, MBD6, and BAP1 [ xref , xref – xref ]."

sparser
"To determine whether MBD5 and MBD6 can also bind to chromatin similar to other BAP1 subunits, we conducted Chromatin Immunopreciptiation Sequencing (ChIP-seq) and determined the chromatin binding profiles of MBD5 and MBD6 with our validated homemade antibodies (Additional file xref : Fig. S1F)."

sparser
"To investigate how MBD5 and MBD6 subunits are incorporated into the BAP1 complex, we first hypothesized that MBD5 and MBD6 may directly interact with BAP1 itself, similar to how other subunits function (e.g., ASXL1-3 and FOXK1/2)."

sparser
"Surprisingly, as shown in Fig. xref B, the protein-protein interaction between MBD5, MBD6, and BAP1 is completely abolished in ASXL-TKO cells (Fig. xref B)."
BAP1 affects KEAP1
| 3 5
BAP1 binds KEAP1.
| 5
| 5

sparser
"Immunoprecipitation of endogenous BAP1 demonstrates that BAP1 interacts with endogenous KEAP1 ( xref D)."

sparser
"In order to identify the domain of KEAP1 that interacts with BAP1, we generated pcDNA3-HA-KEAP1 mutant plasmids lacking individual domains (HA-KEAP1ΔBTB, HA-KEAP1ΔBACK, and HA-KEAP1ΔKELCH)."

sparser
"Alternatively, it can be speculated that BAP1 might competitively bind to KEAP1 at the BTB domain with unknown protein(s) affecting the stability of KEAP1."

sparser
"In order to identify the domains of BAP1 that interact with KEAP1, we generated FLAG-BAP1 mutant plasmids lacking individual domains (FLAG-BAP1ΔCD, FLAG-BAP1ΔCC1, FLAG-BAP1ΔCL, FLAG-BAP1ΔCC2, FLAG-BAP1ΔULD, and FLAG-BAP1ΔCTE)."

sparser
"Our result shows that FLAG-BAP1 does not selectively bind to HA-KEAP1ΔBTB ( xref A, right panel), suggesting that BAP1 binds to the BTB domain of KEAP1."
BAP1 deubiquitinates KEAP1.
| 3
BAP1 deubiquitinates KEAP1. 3 / 3
| 3

reach
"Together, our study demonstrates that KEAP1 deubiquitination by BAP1 is novel tumor suppressive mechanism of LUAD."

reach
"BAP1 Downregulates NRF2 Target Genes and Exerts Anti-Tumorigenic Effects by Deubiquitinating KEAP1 in Lung Adenocarcinoma."

reach
"In the present study, we have identified that BAP1 deubiquitinates KEAP1 by binding to the BTB domain."
BAP1 affects KDM5C
| 8
| 3

sparser
"Mutations of KDM5C and BAP1 were significantly associated with evidence of renal vein invasion (P = ."

sparser
"Mutations of the KDM5C and BAP1 genes were significantly associated with evidence of renal vein invasion on contrast-enhanced CT of ccRCC (p=0.022 and 0.046, respectively) The genotype of solid ccRCC differed significantly from the one of multicystic ccRCC."

sparser
"Mutations of KDM5C and BAP1 were significantly associated with evidence of renal vein invasion (p=0.022 and 0.046, respectively)."
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
BAP1 affects H2AK119ub1
| 1 7
BAP1 deubiquitinates H2AK119ub1.
| 3
BAP1 deubiquitinates H2AK119ub1. 3 / 3
| 3

reach
"XREF_BIBR reported that BAP1 is phosphorylated at serine 592 following DNA damage, that phospho-BAP1 causes deubiquitination of H2AK119ub1, and that this signal promotes the HR pathway."

reach
"87 As Drosophila Calypso, human BAP1 deubiquitylates H2AK119ub1."

reach
"Mammalian BAP1 deubiquitylates H2AK119ub1, 87 and BAP1 reintroduction into BAP1 deficient ccRCC cell lines affected global levels of H2AK119ub1."
BAP1 inhibits H2AK119ub1.
| 1 1
BAP1 inhibits H2AK119ub1. 2 / 2
| 1 1

eidos
"Loss of BAP1 results in a broad increase in H2AK119ub1 levels that is primarily dependent on PCGF3 / 5-PRC1 complexes ."

reach
"Using embryonic stem cells, we show that BAP1 restricts H2AK119ub1 deposition to Polycomb target sites."
BAP1 activates H2AK119ub1.
| 2
BAP1 activates H2AK119ub1. 2 / 2
| 2

reach
"BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation."

reach
"In human tumors, H2AK119ub1 deposition is enhanced by frequent inactivating mutations of the H2AK119ub1-specific deubiquitinase BAP1 (Carbone et al., 2013)."
BAP1 decreases the amount of H2AK119ub1.
| 1
BAP1 decreases the amount of H2AK119ub1. 1 / 1
| 1

reach
"Moreover, BAP1 knockdown leads to increased H2AK119ub1 levels on E2F responsive promoters."
YY1 affects HCFC1
| 2 5
YY1 binds HCFC1 and BAP1. 7 / 7
| 2 5

sparser
"BAP1 forms a ternary complex with HCF1 and YY1, which acts as transcriptional repressor of genes involved in cell proliferation ( xref )."

sparser
"31 Further, BAP1 forms a protein complex with the host cell factor 1 and Yin and Yang 1."

sparser
"3,10,12 BAP1 and HCF-1 can also form a complex with the transcription factor YY1, which recruits BAP1 and HCF-1 to various promoters to regulate gene expression."

reach
"Prominent among the positively enriched gene sets were those related to writers of histone H3K27Me3, including PRC2 and SUZ12 (XREF_FIG), as well as genes participating in cell proliferation and transcription regulation involving ubiquitin signaling mediated by the Bap1, HCF1, and YY1 complex, EED related stem cell pluripotency, cytokine signaling, and cell adhesion; 100 of the top pathways differentially affected in MMs from TKO versus DKO mice are presented in XREF_SUPPLEMENTARY."

reach
"Our RNA-seq analysis revealed that MMs from Bap1; Nf2; Cdkn2a CKO mice exhibit positively enriched gene sets consistent with cellular processes previously implicated in Bap1 function, such as genes mediated by the Bap1, HCF1, and YY1 complex, which play a role in cellular proliferation and transcription regulation involving ubiquitin signaling."

sparser
"Yu H, Mashtalir N, Daou S, et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression."

sparser
"Finally, BAP1 forms complexes with the transcription factors Yin Yang 1 (YY1) and host cell factor 1 (HCF-1), and controls cell-cycle progression at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters [ xref , xref ]."
PTEN affects BAP1
1 | 1 5
1 | 1 3

sparser
"These findings demonstrate that BAP1 is an important deubiquitinase of PTEN for its stability and the BAP1-PTEN signaling axis plays a crucial role in tumor suppression."

sparser
"BAP1 can physically bind to and deubiquitinate PTEN, which inhibits the ubiquitination-mediated degradation of PTEN and thus stabilizes PTEN protein."

reach
"BAP1 can physically bind to and deubiquitinate PTEN, which inhibits the ubiquitination mediated degradation of PTEN and thus stabilizes PTEN protein."

No evidence text available

sparser
"In analyses adjusted only for cohort, mutations in BAP1 (median: 8 vs 5.2 cm; p = 0.001) and PTEN (median: 8 vs 5.9 cm; p = 0.026) were significantly associated with larger median tumor diameter."
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
Histone affects BAP1
| 2 3
| 2 1

sparser
"ASXL3 is less well studied than its paralogs but was shown to promote BAP1 binding to its histone substrate like its paralogs [ xref ]."
| PMC

reach
"Complex formation between BAP1 and the cell cycle regulator, HCF-1, bridges HCF-1 to histone-modifying complexes to regulate cell growth (2,4)."

reach
"We find that ASXL1 directs the binding of the positively charged C-terminal extension of BAP1 to nucleosomal DNA and histones H3-H4 near the dyad, an addition to its role in forming the ubiquitin-binding cleft."
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."
HCFC1 affects YY1
| 2 5
YY1 binds HCFC1 and BAP1. 7 / 7
| 2 5

sparser
"BAP1 forms a ternary complex with HCF1 and YY1, which acts as transcriptional repressor of genes involved in cell proliferation ( xref )."

sparser
"31 Further, BAP1 forms a protein complex with the host cell factor 1 and Yin and Yang 1."

sparser
"3,10,12 BAP1 and HCF-1 can also form a complex with the transcription factor YY1, which recruits BAP1 and HCF-1 to various promoters to regulate gene expression."

reach
"Prominent among the positively enriched gene sets were those related to writers of histone H3K27Me3, including PRC2 and SUZ12 (XREF_FIG), as well as genes participating in cell proliferation and transcription regulation involving ubiquitin signaling mediated by the Bap1, HCF1, and YY1 complex, EED related stem cell pluripotency, cytokine signaling, and cell adhesion; 100 of the top pathways differentially affected in MMs from TKO versus DKO mice are presented in XREF_SUPPLEMENTARY."

reach
"Our RNA-seq analysis revealed that MMs from Bap1; Nf2; Cdkn2a CKO mice exhibit positively enriched gene sets consistent with cellular processes previously implicated in Bap1 function, such as genes mediated by the Bap1, HCF1, and YY1 complex, which play a role in cellular proliferation and transcription regulation involving ubiquitin signaling."

sparser
"Yu H, Mashtalir N, Daou S, et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression."

sparser
"Finally, BAP1 forms complexes with the transcription factors Yin Yang 1 (YY1) and host cell factor 1 (HCF-1), and controls cell-cycle progression at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters [ xref , xref ]."
EZH2 affects BAP1
| 5 2
EZH2 binds BAP1.
| 1 2
| 1 2

sparser
"In these lung-iPSCs, the expression of EZH2 (the main component of PRC2 complex) is also very high, and the physical interactions of AXSL proteins with EZH2 and BAP1 is also reported [ xref , xref , xref ]."

sparser
"Survival analysis revealed that BAP1 loss, but not high EZH2 expression, was associated with a better prognosis."

reach
"We did not identify an interaction between BAP1 and EZH2 by co-immunoprecipitation (XREF_FIG)."
EZH2 inhibits BAP1.
| 2
EZH2 inhibits BAP1. 2 / 2
| 2

reach
"Concomitant Bap1 and Ezh2 loss reduced myeloid progenitor expansion, reduced the proportion of Mac1 + Gr1 + myeloid cells, restored erythroid differentiation (CD71 + Ter119 +) and decreased proliferation of Bap1 and Ezh2 deficient progenitors (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"Ezh2 haploinsufficiency reduced, but did not abrogate, Bap1 deficient myeloproliferation (XREF_SUPPLEMENTARY) consistent with a dose dependent requirement for Ezh2."
EZH2 activates BAP1.
| 2
EZH2 activates BAP1. 2 / 2
| 2

reach
"In BAP1-deficient human mesothelioma cells, EZH2 inhibition also produced synthetic lethality ."

reach
"Interestingly, EZH2 depletion by both genetic and pharmacologic methods abrogated the oncogenic effect of BAP1 loss."
| 7
| 4

reach
"Diminished Pgc1alpha signaling likely contributes to some of the observed phenotypes in Bap1ko mice, but Bap1 likely modulates metabolism by additional means."

reach
"This resulted in a reduced list revealing that BAP1-downregulated genes were enriched in cell metabolism-associated genes."

reach
"Moreover, BAP1 deficiency drives the reprogramming of cell metabolism, promoting anaerobic glycolysis for energy production rather than mitochondrial respiration and increasing extracellular lactate secretion which induces immune evasion, tumour growth and cell malignant transformation.BAP1 emerges as a highly tissue-specific and context-specific tumour suppressor participating to the biology of the tumour with multiple mechanisms and different levels (summary in Table 1)."

reach
"Gene ontology (GO) analysis of 354 BAP1 upregulated and 187 BAP1 downregulated genes revealed that, while BAP1 upregulated genes were enriched in diverse cellular processes (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY), the genes that were downregulated by BAP1 showed striking enrichment in metabolism related biological processes, among which " response to oxidative stress " was the most significantly enriched (XREF_FIG and XREF_SUPPLEMENTARY)."
BAP1 activates metabolic process.
| 3

reach
"BAP1 Modulates Cellular Metabolism."

reach
"In non-tumoral cells, reduced BAP1 levels induced the Warburg effect, a shift of cell metabolism from oxidative phosphorylation (Krebs cycle) to aerobic glycolysis, originally identified as typical of cancer cells."

reach
"As a deubiquitinating enzyme, BAP1 contributes to gene transcription, cell differentiation, DNA damage repair, apoptosis, and cell metabolism in tumor inhibition."
| 1 6
BAP1 inhibits cell migration.
| 1 3
| 1 3

reach
"However, BAP1 is closely related to lymph node status, suggesting that BAP1 also mediates suppression of cell migration in LAC which is in keeping with the findings of the wound healing assay."

reach
"Knockdown of BAP1 and KLF5 significantly decreased cell migration compared with the Lucsh negative control in HCC1806 and HCC1937 cells using wound healing and transwell assays (XREF_FIG; XREF_SUPPLEMENTARY)."

eidos
"We found that BaP1 is selective for CRC cells and reduces cell proliferation , cell survival , and cell migration ."

reach
"The overexpression of CAST rescued the cell migration phenotype caused by BAP1 loss."
BAP1 activates cell migration.
| 3
| 3

reach
"We found that dual knockdown of BAP1 and miR-31 significantly reversed cell migration ability of HeLa and C33A cells induced by single-knockdown of miR-31."

reach
"Upregulation of miR-31 and Knockdown of BAP1 Promoted Cell Migration In Vitro."

reach
"BAP1 depletion appears to promote UM cell migration underneath the monolayer."
BAP1 affects calcium(2+)
| 7
BAP1 increases the amount of calcium(2+).
| 4
BAP1 increases the amount of calcium(2+). 4 / 4
| 4

reach
"A recent factor that deserves attention is the tumour suppressor BAP1, which has been shown to increase the amount of Ca 2+ released from the ER into the cytosol and mitochondria, promoting apoptosis XREF_BIBR and increasing aerobic glycolysis XREF_BIBR."

reach
"Additionally, BAP1 promotes tumor cell apoptosis by regulating ER-mitochondrial calcium release (19)."

reach
"Although the exact mechanism underpinning the metabolic alterations associated with BAP1 loss are currently unknown, a study from 2017 reported that BAP1 promotes calcium (Ca ) release from the ER to the mitochondria ."

reach
"In another report, BAP1 was found to modulate endoplasmic reticulum (ER)-mitochondria calcium release (4)."
BAP1 activates calcium(2+).
| 3
| 3

reach
"(ii) The tumor suppressor BRCA1 associated protein 1 (BAP1), a deubiquitylating enzyme, promotes ER-mitochondrial Ca 2+ transfer by stabilizing the IP 3 R3 114."

reach
"In a paper in press in Nature, 19 we report that BAP1 modulates ER-to-mitochondria calcium (Ca 2+) release."

reach
"In a paper in press in Nature, 19 we report that BAP1 modulates ER-to-mitochondria calcium (Ca 2+) release."
BAP1 affects UBE2O
5 | 1 1
5 | 1 1

No evidence text available

No evidence text available

No evidence text available

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sparser
"Since BAP1 5K/R mutant still interact with UBE2O and is ubiquitinated on other sites, we also replaced the NLS region of BAP1, containing the UBE2O-interacting motif, with T-large antigen NLS which pr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"For example, UBE2O binds to and multi-monoubiquitinates BAP1 to induce its cytoplasmic localization and promotes adipocyte differentiation [XREF_BIBR]."
BAP1 affects PPARGC1A
| 6 1
BAP1 binds PPARGC1A.
| 2 1
| 2 1

reach
"PUGNAc treatment promotes the interaction between PGC-1alpha and BAP1, while it has no effect on the S333A mutant (XREF_FIG), suggesting that O GlcNAcylation of PGC-1alpha facilitates BAP1 binding."

reach
"38 Furthermore, the authors found that high-glucose conditions potentiated PGC1alpha glycosylation, suggesting that this O-GlcNAc-induced PGC1alpha and BAP1 complex may provide a functional explanation for the aberrant induction of hepatic gluconeogenesis in hyperglycemic patients."

sparser
"Conversely, O -GlcNAcylation of the transcriptional regulator PGC1α facilitates the binding of the deubiquitinase BAP1 to PGC1α, leading thus to its stabilization by blocking its ubiquitination ( xref )."
BAP1 increases the amount of PPARGC1A.
| 2
Modified BAP1 increases the amount of PPARGC1A. 2 / 2
| 2

reach
"In line with our observation that BAP1 is a deubiquitinase for PGC-1alpha in vitro, hepatic BAP1 overexpression increases the protein level of PGC-1alpha (XREF_FIG)."

reach
"Overexpression of BAP1 does not increase global ubiquitination, but substantially increases the level of PGC-1alpha and decreases PGC-1alpha ubiquitination as normalized to the protein level (XREF_FIG)."
BAP1 activates PPARGC1A.
| 2
BAP1 activates PPARGC1A. 2 / 2
| 2

reach
"Bap1 overexpression in the liver slightly enhances gluconeogenesis, possibly by stabilizing Pgc1alpha."

reach
"Conversely, knockdown of BAP1 in cells (XREF_SUPPLEMENTARY) decreases the stability of PGC-1alpha (XREF_FIG)."
BAP1 affects HDAC2
| 2 2
BAP1 inhibits HDAC2.
| 1
BAP1 inhibits HDAC2. 1 / 3
| 1

reach
"In addition, we found that BAP1 depletion did not reduce the stability of residual HDAC2 under conditions where translation was blocked."
BAP1 decreases the amount of HDAC2.
| 1
BAP1 decreases the amount of HDAC2. 1 / 2
| 1

reach
"We found that depletion of the tumor suppressor BAP1 reduced HDAC2 expression, acting at the level of transcription rather than ubiquitylation."
BAP1 activates HDAC2.
| 2
BAP1 activates HDAC2. 2 / 2
| 2

eidos
"Another relevant study demonstrated that BAP1 can transcriptionally promote the expression of HDAC2 ."
| PMC

eidos
"The BAP1 knockdown led to a decrease in HDAC2 but an increase in HDAC1 expression ."
| PMC
BAP1 affects HCFC1, and YY1
| 2 5
YY1 binds HCFC1 and BAP1. 7 / 7
| 2 5

sparser
"BAP1 forms a ternary complex with HCF1 and YY1, which acts as transcriptional repressor of genes involved in cell proliferation ( xref )."

sparser
"31 Further, BAP1 forms a protein complex with the host cell factor 1 and Yin and Yang 1."

sparser
"3,10,12 BAP1 and HCF-1 can also form a complex with the transcription factor YY1, which recruits BAP1 and HCF-1 to various promoters to regulate gene expression."

reach
"Prominent among the positively enriched gene sets were those related to writers of histone H3K27Me3, including PRC2 and SUZ12 (XREF_FIG), as well as genes participating in cell proliferation and transcription regulation involving ubiquitin signaling mediated by the Bap1, HCF1, and YY1 complex, EED related stem cell pluripotency, cytokine signaling, and cell adhesion; 100 of the top pathways differentially affected in MMs from TKO versus DKO mice are presented in XREF_SUPPLEMENTARY."

reach
"Our RNA-seq analysis revealed that MMs from Bap1; Nf2; Cdkn2a CKO mice exhibit positively enriched gene sets consistent with cellular processes previously implicated in Bap1 function, such as genes mediated by the Bap1, HCF1, and YY1 complex, which play a role in cellular proliferation and transcription regulation involving ubiquitin signaling."

sparser
"Yu H, Mashtalir N, Daou S, et al. The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression."

sparser
"Finally, BAP1 forms complexes with the transcription factors Yin Yang 1 (YY1) and host cell factor 1 (HCF-1), and controls cell-cycle progression at G1/S by co-regulating transcription from HCF-1/E2F-governed promoters [ xref , xref ]."
BAP1 affects DNA Damage
| 7
| 6

reach
"BAP1 modulates double-strand DNA damage repair."

reach
"BAP1 can deubiquitylate Ub-H2AK119 at the site of UV-induced DNA damage, and promote DNA repair by regulating the accumulation of repair proteins (BRCA1, RAD51, and RPA) (7)."

reach
"Here, we show that BAP1 promotes the repair of ultraviolet (UV)-induced DNA damage via its deubiquitination activity in various cell types, including primary melanocytes."

reach
"In summary, reduced BAP1 levels cause cells to accumulate DNA damage, a process that normally triggers apoptosis."

reach
"In heterozygous BAP1 conditions, as in the individuals of the families with the BAP1 cancer syndrome, the reduced BAP1 dosage impairs both the DNA repair, accumulating DNA damage, and the apoptotic response."

reach
"Molecularly, Bap1 null melanoma cell lines have increased DNA damage measured by gammaH2aX and hyperubiquitination of histone H2a."
Mutated BAP1 activates DNA Damage. 1 / 1
| 1

reach
"However, the spontaneous development of mesotheliomas in Bap1 +/- mice not exposed to asbestos, and the development of multiple cancer types in carriers of BAP1 mutations (XREF_FIG), including tumor types that have not been associated with known carcinogens, suggests that BAP1 mutations also drive tumor growth independently of genotoxic stress, perhaps by favoring the accumulation of age related DNA damage."
BAP1 affects ASXL
| 7
BAP1 binds ASXL. 7 / 7
| 7

reach
"To be noticed, both leukemias and UMs associated with MBD4 inactivation share a consistent inactivation of the BAP1 and ASXL complex."

reach
"This was in congruence with the fact that BAP1 binds to ASXL to form the Polycomb repressive deubiquitinase complex (PR-DUB) that deubiquitinates histone H2A [XREF_BIBR]."

reach
"BAP1 and ASXL complex modulation regulates epithelial-mesenchymal transition during trophoblast differentiation and invasion."

reach
"Human ASXL1 is mutated frequently in chronic myelomonocytic leukemia (CMML) so an ASXL and BAP1 complex may suppress CMML."

reach
"Our data raise the possibility that ASXL1 truncation mutations confer gain-of-function on the ASXL and BAP1 complex."

reach
"This was in congruence with the fact that BAP1 binds to ASXL to form the Polycomb repressive deubiquitinase complex (PR-DUB) that deubiquitinates histone H2A [73]."

reach
"Although H2Aub was previously involved in cell proliferation , it is not clear, at this time, whether the observed effects of ASXL2/BAP1 defective in monoubiquitination are solely due to disruption of H2A deubiquitination or the effect of BAP1/ASXL complexes on other substrates.Our data suggest that the signaling pathway we characterized here is important for tumor suppression."
N1ICD affects BAP1
| 6
BAP1 binds N1ICD. 6 / 6
| 6

sparser
"Moreover, the presence of full-length NUMB, but not the AB-deleted NUMB, significantly enhances the interaction between BAP1 and N1ICD."

sparser
"Next, we asked whether BAP1 is associated with N1ICD."

sparser
"Parallelly, protein levels of activated NOTCH1 was found to be highly expressed in cortical NPCs ( xref ), and BAP1 associates with endogenous activated N1ICD in cortical NPCs ( xref )."

sparser
"In a more stringent assay, the interaction between N1ICD and endogenous BAP1 was validated by FLAG-bound IP in HeLa N1ICD-FLAG cells ( xref )."

sparser
"Finally, NUMB overexpression enhanced the interaction between BAP1 and N1ICD ( xref ), but co-expression of non-N1ICD-bound NUMB-∆2–15 could not promote BAP1N1ICD association, thus failing to boost stabilization of N1ICD ( xref )."

sparser
"Interestingly, we found that the RAM–ANK and PEST domains of N1ICD, regions required for NUMB association, mediate N1ICD interaction with BAP1 as well ( xref ), and BAP1 regulates N1ICD stability through the PEST domain ( xref )."
BRCA1 affects BARD1
| 1 5
BRCA1 binds BAP1 and BARD1. 6 / 6
| 1 5

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear and needs to be studied in more detail."

sparser
"BAP1 interacts with BARD1 and BRCA1 and inhibits the E3 ligase activity of BRCA1/BARD1."

sparser
"The ring domains of BRCA1 (residues 1-109) are associated with BARD1 [ xref ] and BAP1 [ xref ]."

sparser
"BAP1 and BRCA1-BARD1 complex coordinately regulate ubiquitination during the DNA damage response and the cell cycle [ xref , xref ]."

reach
"Until recently, the combination of pemetrexed and cisplatin was the only approved first-line therapy, yielding a median overall survival (OS) of 12.1 months.2 Nivolumab plus ipilimumab was approved recently as initial treatment for malignant pleural mesothelioma (MPM) on the basis of a phase 3 clinical trial that resulted in a median OS of 18.1 months.3 4 There are no approved therapies for patients with tumor relapse after first-line therapies.Risk factors in developing mesothelioma include exposure to asbestos5 and other carcinogenic fibers such as erionite,6 and previous radiation therapy.7 8 Studies of familial clusters of mesothelioma have revealed genetic predisposition to develop MM.9 10 Recent work has identified germline mutations in BAP1 that can predispose to mesothelioma11 12 and other cancers such as uveal and cutaneous melanoma, basal cell carcinoma, meningioma, and renal cell cancers.13 14 We previously identified deleterious germline mutations in 12% of 241 consecutive patients with MM who enrolled on a prospective natural history study, with BAP1 being the most frequent germline mutation.15 BAP1 was also recurrently inactivated at the somatic level, suggesting BAP1 variants undergoing positive selection in the context of the classic “two-hit” model.BAP1, a nuclear deubiquitylase, was initially discovered as an interaction partner of the tumor suppressor BRCA1.16 Subsequent studies revealed that BAP1 binds to BARD1, which, in turn, binds to BRCA1, forming the BRCA1-BARD1-BAP1 complex that is crucial for the homologous recombination (HR)–mediated repair of DNA double-strand breaks (DSBs).17 PARP enzymes play a major role in DNA single-strand break repair and base excision repair pathways."

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear."
| 6
| 6

reach
"Because exogenously provided Bap1 restored biofilm surface adhesion to a Deltabap1DeltarbmC mutant, we questioned whether Bap1 synthesis could be a joint venture in the biofilm community."

reach
"This suggests that communal Bap1 secreted by nearby biofilm cells may also increase surface adhesion of bystanders that have not yet been reprogrammed for biofilm matrix synthesis."

reach
"Two of the proteins, RbmC and Bap1, are seemingly redundant paralogs that coat substrate surfaces and promotes cell adhesion, while another protein RbmA coats cell surfaces and promotes cell-cell cohesion."

reach
"Bap1 mediates adhesion of bystander cells."

reach
"As shown in XREF_FIG and quantified in XREF_FIG, Bap1 increased surface adhesion of wild-type V. cholerae, a Deltabap1DeltarbmC mutant, and a DeltavpsL mutant in monolayer minimal medium, while a control protein, bovine serum albumin (BSA), had no effect."

reach
"Exogenous Bap1 increases surface adhesion of planktonic bystanders as well."
BAP1 affects TNFSF10
| 6
BAP1 activates TNFSF10.
| 4
BAP1 activates TNFSF10. 4 / 4
| 4

reach
"rTRAIL sensitivity of the parental BAP1-null H226 MM line was significantly diminished following expression of wild-type BAP1 and each of the mutant constructs except those with an inactive deubiquitinating or ASXL protein binding site (XREF_FIG), implicating the function of these sites in BAP1 induced TRAIL resistance."

reach
"BAP1 modulates TRAIL sensitivity through PR-DUB activity."

reach
"While we highlight the extrinsic apoptotic pathway and proteins as being significantly altered by BAP1 status, identifying a single factor to explain BAP1 induced TRAIL resistance is extremely challenging."

reach
"To elucidate the mechanism by which BAP1 modulates sensitivity to TRAIL we generated expression vectors containing wild-type or mutant forms of BAP1, each with an inactive functional site or protein binding domain."
BAP1 decreases the amount of TNFSF10.
| 2
BAP1 decreases the amount of TNFSF10. 2 / 2
| 2

reach
"As a potential underlying mechanism, here we report that BAP1 negatively regulates the expression of TRAIL receptors: death receptors 4 (DR4) and 5 (DR5)."

reach
"These results suggest that BAP1 and YY1 cooperatively repress transcription of TRAIL receptors."
BAP1 affects TERT
| 3 3
BAP1 decreases the amount of TERT.
| 3
BAP1 decreases the amount of TERT. 3 / 3
| 3

reach
"In conclusion, the results from the present study provide evidence to suggest that BAP1 and possibly PARP-3 repress hTERT transcription within breast cancer cells, which supports the hypothesis that multiple sequences on human chromosome 3p may be responsible for regulating hTERT transcription."

reach
"Interestingly, literature data suggest that BAP1 could downregulate TERT expression (Linne et al., 2017)."

reach
"Chromosome 3p21.1-21.3 encoded sequences BAP1, but not SETD2 PARP-3 and PBRM1, repress hTERT transcription within 21NT cells."
BAP1 binds TERT.
| 3
| 3

sparser
"The presence of BAP1 or TERT promoter mutations was associated with a significantly worse OS."

sparser
"An exploratory analysis was performed to investigate if acquired mutations in BAP1 or TERT were associated with MSKCC prognostic risk group or the presence of sarcomatoid features on histopathologic review."

sparser
"Shorter overall survival was associated with TERT mutations ( p  = 0.03, median 29.6 months for MT versus 52.6 months for WT) or BAP1 mutations ( p  = 0.02, median 28.7 months for MT versus not reached for WT)."
BAP1 affects PTGES
| 6
BAP1 increases the amount of PTGES. 6 / 6
| 6

reach
"Gene and protein expression of both COX-2 and mPGES-1 are induced by BaP1 via activation of distinct subunits of the NF-κB transcription factor."

reach
"We found that the EP4 receptor positively regulates BaP1-triggered PGE release and expression of COX-2 and mPGES-1, which are critically involved in the biosynthesis of PGE induced by this metalloprotease."

reach
"Accordingly, our data show that BaP1 induced a marked increase in protein expression of both COX-2 and mPGES-1, the increase in COX-2 expression occurring before that of mPGES-1."

reach
"As shown in Fig. 5, pretreatment of cells with SN50 inhibited BaP1-induced COX-2 protein expression (Fig. 5C,D) and BaP1-induced mPGES-1 protein expression (Fig. 5G,H)."

reach
"Expression of COX-2 and mPGES-1 are induced by BaP1 via activation of NF-kappaB pathway."

reach
"Furthermore, our findings that BaP1 induced gene expression of COX-2 and mPGES-1 demonstrate that BaP1 can trigger transcriptional mechanisms in FLSs that result in increased protein expression."
BAP1 affects PRC2_complex
| 3 3
| 1 3

sparser
"In addition, BAP1 mutations in ccRCC tumors were associated with downregulated expression of target genes of polycomb repressive complex 2 (PRC2) [ xref ] ( xref )."

sparser
"Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells."

sparser
"BAP1 does not interact with PRC2 proteins ( xref )."

reach
"Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells."
BAP1 inhibits PRC2_complex.
| 2
| 2

reach
"Loss of BAP1 has been shown to be synthetically lethal with inhibition of EZH2 or PRC2."

reach
"As for the reported tumor suppressive mechanism, BAP1 can antagonize EZH2 and PRC2 or RING1B (RNF2)/PRC1 in a tissue specific manner."
BAP1 affects N1ICD
| 6
BAP1 binds N1ICD. 6 / 6
| 6

sparser
"Moreover, the presence of full-length NUMB, but not the AB-deleted NUMB, significantly enhances the interaction between BAP1 and N1ICD."

sparser
"Next, we asked whether BAP1 is associated with N1ICD."

sparser
"Parallelly, protein levels of activated NOTCH1 was found to be highly expressed in cortical NPCs ( xref ), and BAP1 associates with endogenous activated N1ICD in cortical NPCs ( xref )."

sparser
"In a more stringent assay, the interaction between N1ICD and endogenous BAP1 was validated by FLAG-bound IP in HeLa N1ICD-FLAG cells ( xref )."

sparser
"Finally, NUMB overexpression enhanced the interaction between BAP1 and N1ICD ( xref ), but co-expression of non-N1ICD-bound NUMB-∆2–15 could not promote BAP1N1ICD association, thus failing to boost stabilization of N1ICD ( xref )."

sparser
"Interestingly, we found that the RAM–ANK and PEST domains of N1ICD, regions required for NUMB association, mediate N1ICD interaction with BAP1 as well ( xref ), and BAP1 regulates N1ICD stability through the PEST domain ( xref )."
BAP1 affects Histone_H2A
| 6
BAP1 ubiquitinates Histone_H2A.
| 3
BAP1 ubiquitinates Histone_H2A. 3 / 3
| 3

reach
"BRCA1-associated protein 1 (BAP1) [18], the crucial constituent of the deubiquitinase complex, attenuated the initiation and extension of SLC7A11 transcription by deubiquitinating histone 2A, while the steady-state levels and half-life of SLC7A11 were improved by combination with the ovarian tumor family member deubiquitinase (OTUB1) [19]."

reach
"Several studies have revealed that BAP1 can inhibit ubiquitinated histone 2A (H2Aub) occupancy on the SLC7A11 promoter (Zhang et al., 2018)."

reach
"Genomic analyses together with robust statistics have revealed that the restoration of BAP1 facilitates the formation of the polycomb repressive deubiquitinase (PR-DUB) complex and inhibits ubiquitinated histone 2A (H2Aub) occupancy on the SLC7A11 promoter."
BAP1 deubiquitinates Histone_H2A.
| 3
BAP1 deubiquitinates Histone_H2A. 3 / 3
| 3

reach
"BRCA1-associated protein 1 (BAP1) [18], the crucial constituent of the deubiquitinase complex, attenuated the initiation and extension of SLC7A11 transcription by deubiquitinating histone 2A, while the steady-state levels and half-life of SLC7A11 were improved by combination with the ovarian tumor family member deubiquitinase (OTUB1) [19]."

reach
"BAP1 deubiquitinates histone 2A in the regulatory chromatin of the of SLC7A11 gene, which encodes, a key transporter of extracellular cysteine."

reach
"The mechanism may be that BAP1 represses the transcription of SLC7A11 by reducing histone 2A ubiquitination (H2Aub) on the SLC7A11 gene (49)."
BAP1 affects HIF1A
| 1 2 3
BAP1 binds HIF1A.
| 1 3
| 1 3

sparser
"We demonstrated that during hypoxia, BAP1 binds, deubiquitylates, and stabilizes HIF-1α, the master regulator of the hypoxia response and tumor cell invasion."

sparser
"We found that BAP1 binds to the N-terminal region of HIF-1α, where HIF-1α binds DNA and dimerizes with HIF-1β forming the heterodimeric transactivating complex HIF."

reach
"HIF1alpha-dependent metabolism:. Bioinformatics interrogation of The Cancer Genome Atlas (TCGA) data have associated BAP1 alterations with changes in hypoxia-inducible factor-1 alpha (HIFα) expression (25,76,77)."

sparser
"In addition, we observed that hypoxic conditions increase exogenous BAP1 expression, raising the possibility of future studies to investigate HIF1α-BAP1 cross-talk, and its role in metabolic alterations."
BAP1 activates HIF1A.
| 1 1
BAP1 activates HIF1A. 2 / 2
| 1 1

reach
"It has been proposed that BAP1 may lead to HIF-1alpha upregulation via the NF-kappaB pathway [XREF_BIBR]."

eidos
"It has been proposed that BAP1 may lead to HIF-1alpha upregulation via the NF-kappaB pathway [ 228 ] ."
BAP1 affects E3_Ub_ligase
| 2 4
BAP1 inhibits E3_Ub_ligase.
| 2 1
| 2 1

reach
"XREF_BIBR suggested that BAP1 inhibits the E3 ligase activity of BRCA1 in a manner independent of its deubiquitination activity."

sparser
"In this regard, however, Nishikawa et al . xref suggested that BAP1 inhibits the E3 ligase activity of BRCA1 in a manner independent of its deubiquitination activity."

reach
"The BRCA1 associated protein 1 (BAP1), a de-ubiquitinating enzyme (DUB), also inhibits the E3 ligase activity of BRCA1-BARD1 (Nishikawa et al., 2009)."
| 3

sparser
"They found that BAP1 did not appear to be involved in the deubiquitination of the BRCA1/BARD1 complex,29 and BAP1-mediated growth suppression in vitro was shown to be independent of wild-type BRCA1.4 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"According to this hypothesis, Nishikawa et al showed that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of the BRCA1/BARD1 complex, and that BAP1 and BRCA1/BARD1 mutually regulate ubiquitination in the cell cycle and in the DNA damage response pathway. xref According to the present state of knowledge, it is reasonable to speculate that BAP1 may exert a regulatory role in the DNA damage response."

sparser
"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1/BARD1."

reach
"BAP1 can modulate the DDR via several different interactions, including deubiquitinylation of histone H2A."

reach
"In such an environment, an impaired DDR caused by BAP1 deletion, might be expected to favour the accumulation of genetic damage that eventually gives rise to a malignant clone."

eidos
"Thus , BAP1 is thought to modulate DDR through H2Aub ."

reach
"Thus, BAP1 is thought to modulate DDR through H2Aub."

eidos
"BAP1 can modulate the DDR via several different interactions , including deubiquitinylation of histone H2A ( 6 , 7 ) ."

reach
"Indeed, inhibition of BAP1 by short hairpin RNAs (shRNAs) impaired the DDR and caused HeLa cells to become hypersensitive to ionizing radiation resulting in S-phase retardation, a phenotype similar to BRCA1 deficiency."
BAP1 affects BARD1, and BRCA1
| 1 5
BRCA1 binds BAP1 and BARD1. 6 / 6
| 1 5

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear and needs to be studied in more detail."

sparser
"BAP1 interacts with BARD1 and BRCA1 and inhibits the E3 ligase activity of BRCA1/BARD1."

sparser
"The ring domains of BRCA1 (residues 1-109) are associated with BARD1 [ xref ] and BAP1 [ xref ]."

sparser
"BAP1 and BRCA1-BARD1 complex coordinately regulate ubiquitination during the DNA damage response and the cell cycle [ xref , xref ]."

reach
"Until recently, the combination of pemetrexed and cisplatin was the only approved first-line therapy, yielding a median overall survival (OS) of 12.1 months.2 Nivolumab plus ipilimumab was approved recently as initial treatment for malignant pleural mesothelioma (MPM) on the basis of a phase 3 clinical trial that resulted in a median OS of 18.1 months.3 4 There are no approved therapies for patients with tumor relapse after first-line therapies.Risk factors in developing mesothelioma include exposure to asbestos5 and other carcinogenic fibers such as erionite,6 and previous radiation therapy.7 8 Studies of familial clusters of mesothelioma have revealed genetic predisposition to develop MM.9 10 Recent work has identified germline mutations in BAP1 that can predispose to mesothelioma11 12 and other cancers such as uveal and cutaneous melanoma, basal cell carcinoma, meningioma, and renal cell cancers.13 14 We previously identified deleterious germline mutations in 12% of 241 consecutive patients with MM who enrolled on a prospective natural history study, with BAP1 being the most frequent germline mutation.15 BAP1 was also recurrently inactivated at the somatic level, suggesting BAP1 variants undergoing positive selection in the context of the classic “two-hit” model.BAP1, a nuclear deubiquitylase, was initially discovered as an interaction partner of the tumor suppressor BRCA1.16 Subsequent studies revealed that BAP1 binds to BARD1, which, in turn, binds to BRCA1, forming the BRCA1-BARD1-BAP1 complex that is crucial for the homologous recombination (HR)–mediated repair of DNA double-strand breaks (DSBs).17 PARP enzymes play a major role in DNA single-strand break repair and base excision repair pathways."

sparser
"Therefore, the association of BAP1 with BRCA1 and BARD1 is currently unclear."
TP53 affects BAP1
| 5
| 3

sparser
"Concurrent mutations in BAP1 and TP53 were associated with adverse outcome, with similar effects observed for acquired alterations in either of the two genes alone ( xref )."

sparser
"Mutations in BAP1 ( q = 0.004) and TP53 ( q = 0.001) were associated with decreased CSS in a multivariable model; only TP53 ( q = 0.005) remained significant when SSIGN score was included."

sparser
"On analyses adjusted only for cohort, we found that mutations in BAP1 ( n = 1049 for analysis; hazard ratio [HR]: 2.10; 95% confidence interval [CI]: 1.44–3.04; q < 0.001) and TP53 ( n = 784 for analysis; HR: 2.63; 95% CI: 1.36–5.08; q = 0.028) were significantly associated with increased risk of death from cancer after adjustment for multiple comparisons using competing risks regression ( xref )."
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
TERT affects BAP1
| 2 3
TERT binds BAP1.
| 3
| 3

sparser
"The presence of BAP1 or TERT promoter mutations was associated with a significantly worse OS."

sparser
"An exploratory analysis was performed to investigate if acquired mutations in BAP1 or TERT were associated with MSKCC prognostic risk group or the presence of sarcomatoid features on histopathologic review."

sparser
"Shorter overall survival was associated with TERT mutations ( p  = 0.03, median 29.6 months for MT versus 52.6 months for WT) or BAP1 mutations ( p  = 0.02, median 28.7 months for MT versus not reached for WT)."
TERT increases the amount of BAP1.
| 2
TERT increases the amount of BAP1. 2 / 2
| 2

reach
"Chromosome 3p21.1-21.3 encoded sequences BAP1, but not SETD2 PARP-3 and PBRM1, repress hTERT transcription within 21NT cells."

reach
"In conclusion, the results from the present study provide evidence to suggest that BAP1 and possibly PARP-3 repress hTERT transcription within breast cancer cells, which supports the hypothesis that multiple sequences on human chromosome 3p may be responsible for regulating hTERT transcription."
SF3B1 affects BAP1
| 5
| 3

sparser
"Uveal melanoma (UM) is a relative rare disease and has a high mortality rate due to metastasis in about half of all patients within 15 y after diagnosis. xref , xref , xref It is the most common primary intra‐ocular malignancy in adults in the Western world. xref UM specific mutations in the alpha subunit genes GNAQ and GNA11 are described as well as mutations in BAP1 , SF3B1 , and EIF1AX . xref , xref , xref Mutations in the latter 3 genes are found in ∼75% of all UM and are useful for prognostication of patients. xref , xref , xref BAP1 ‐mutated UM gives rise to early‐onset metastasis whereas SF3B1 ‐mutated UM gives rise to late‐onset metastasis and EIF1AX ‐mutated UM hardly metastasizes. xref Mutations in PLCB4 and CYSLTR2 are described in UM in a mutually exclusive manner to GNAQ or GNA11 mutations but so far have not been associated with prognosis. xref , xref Copy number alterations in chromosomes 1, 3, 6, and 8 are correlated with prognosis of the UM patient. xref , xref UM with EIF1AX , SF3B1 and BAP1 mutations are associated with unique chromosomal patterns, suggesting distinct UM subclasses."

sparser
"The Rotterdam Ocular Melanoma Study Group investigated the association of EIF1AX , SF3B1 and BAP1 mutation with disease-free survival and metastatic risk of patients with uveal melanoma. xref In their recent article, they published a Kaplan-Meier survival probability curve regarding these mutations."

sparser
"Other significantly mutated genes in UM are BAP1 , EIF1AX and SF3B1 , which also form a second mutually‐exclusive subgroup xref (Table  xref )."
| 2

sparser
"To further investigate the genetic interaction between BAP1 and SF3B1 mutations and to exclude that other alterations in Mel202 might contribute to the incompatibility with BAP1 deletion, we asked whether deletion of the SF3B1 ‐mutant allele in Mel202 cells could reverse BAP1 KO‐induced growth arrest."

sparser
"That study highlighted the role of coding and non-coding genes that were differentially expressed between SCNA subtypes of monosomy 3 uveal melanomas that were associated with metastasis. xref Five genes have been reported to be frequently mutated in uveal melanoma. xref Mutations in GNAQ and GNA11 occur early in tumor formation while BAP1 , SF3B1 , and EIF1AX mutations likely occur later in tumor progression. xref Mutation in EIF1AX is an indicator of good prognosis, whereas mutations in SF3B1 and BAP1 are associated with intermediate and poor prognosis."
SETD2 affects KDM5C
| 5
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
PARP3 affects BAP1
| 1 4
PARP3 binds BAP1.
| 4
| 4

sparser
"Thus, we suspect that the novel interaction of BAP1 and PARP3 may also be perturbed by PARP inhibitors, leading to inhibition of cancer growth."

sparser
"In this scenario, it is possible that the novel interaction of BAP1 with PARP3 in cancerous cells may be promoting cancerous growth, possibly through regulation of DNA repair and apoptosis."

sparser
"Several studies and clinical trials [ xref ], have shown that PARP inhibitors influence cancers in which mutations in BRCA1 or BRCA2 are observed, which led us to assume that the cancerous growth-inhibiting interaction of BAP1 with BRCA1 may already be perturbed in this case, and that PARP inhibitors may actually be blocking the novel interaction of BAP1 with PARP3 which enhances cancer growth."

sparser
"Perturbation of the interaction of BAP1 with PARP3 , using PARP3 inhibitors, may then suppress cancerous growth, at least in sarcomatoid MPM."
PARP3 activates BAP1.
| 1
PARP3 activates BAP1. 1 / 1
| 1

reach
"In the present study, overexpression of BAP1, but not PARP3, PBRM1 or SETD2, was associated with significant (but not complete) repression of hTERT transcription within 21NT breast cancer cells."
| 5

sparser
"BAP1 knockdown in MM cell lines has been paradoxically associated to a decreased proliferation, with an accumulation of cells in the S phase of the cell cycle xref , suggesting that BAP1 loss might promote tumorigenesis inducing a delayed, but more permissive, G1/S checkpoint xref ."

sparser
"BRCA1-associated protein-1 ( BAP1 ) mutations is known to be related to a high rate of MM and MM associated with germline BAP1 mutations has a better prognosis of overall 7-fold increased long-term s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The association of germline BAP1 mutations with familial MM has been confirmed in a series of recent reports ( xref - xref )."

sparser
"Here, we tested the hypothesis that MM associated with germline BAP1 mutations has a better prognosis compared with sporadic MM."

sparser
"The demonstration that BAP1 mutations are strongly associated with MM has provided a real breakthrough in the study of genetic predisposition in MM, that may explain why only a fraction of asbestos-exposed individuals go on to develop MM."
KEAP1 affects BAP1
| 5
| 5

sparser
"Immunoprecipitation of endogenous BAP1 demonstrates that BAP1 interacts with endogenous KEAP1 ( xref D)."

sparser
"In order to identify the domain of KEAP1 that interacts with BAP1, we generated pcDNA3-HA-KEAP1 mutant plasmids lacking individual domains (HA-KEAP1ΔBTB, HA-KEAP1ΔBACK, and HA-KEAP1ΔKELCH)."

sparser
"Alternatively, it can be speculated that BAP1 might competitively bind to KEAP1 at the BTB domain with unknown protein(s) affecting the stability of KEAP1."

sparser
"In order to identify the domains of BAP1 that interact with KEAP1, we generated FLAG-BAP1 mutant plasmids lacking individual domains (FLAG-BAP1ΔCD, FLAG-BAP1ΔCC1, FLAG-BAP1ΔCL, FLAG-BAP1ΔCC2, FLAG-BAP1ΔULD, and FLAG-BAP1ΔCTE)."

sparser
"Our result shows that FLAG-BAP1 does not selectively bind to HA-KEAP1ΔBTB ( xref A, right panel), suggesting that BAP1 binds to the BTB domain of KEAP1."
KDM5C affects SETD2
| 5
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
KDM1B affects BAP1
4 | 1
4 | 1

No evidence text available

sparser
"In addition, the mass spectrometric analysis by xref also identified a physical interaction between BAP1 and the H3K4 demethylase KDM1B (also known as LSD1B), an intriguing interaction that might implicate BAP1/ASXL in KDM1B function and will need to be validated further."

No evidence text available

No evidence text available

No evidence text available
INO80 affects BAP1
1 | 3 1
1 | 3 1

reach
"BAP1 interacts with and deubiquitylates INO80, playing a critical role in stabilizing and targeting the INO80 chromatin remodeling complex to the replication forks."

reach
"A recent study demonstrates that Ino80 interacts with BRCA1 associated protein-1 (BAP1), a tumor suppressor that also stabilizes Ino80, in normal DNA replication [XREF_BIBR]."

reach
"Both BAP1 and INO80 bind chromatin at replication forks upon HU treatment."

sparser
"Both BAP1 and INO80 bind chromatin at replication forks upon HU treatment."

No evidence text available
H2AC20 affects BAP1
3 | 2
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."
GEP affects BAP1
| 5
BAP1 binds GEP. 3 / 3
| 3

sparser
"Inactivating mutations in the tumor suppressor BAP1 are associated with the class 2 GEP and high metastatic risk xref , whereas single nucleotide substitutions in SF3B1 and EIF1AX are found mainly in class 1 tumors and are associated with intermediate and low metastatic risk, respectively xref , xref ."

sparser
"Mutations in SF3B1 and EIF1AX are associated with Class 1 GEP and have been reported to be good prognostic factors in UM, whereas BAP1 mutations are associated with Class 2 GEP and poor prognosis ( xref , xref )."

sparser
"BAP1 mutations were associated with class 2 GEP ( P < ."
BAP1 binds GEP and class 2. 2 / 2
| 2

sparser
"12,36,39 BAP1 mutation is associated with monosomy 3 or class 2 GEP tumors and impart poor survival."

sparser
"BAP1 is associated with monosomy 3, poor prognosis, and class 2 GEP tumors, while EIF1AX is associated with class 1 GEP tumors and good prognosis."
| 2 3
BAP1 translocates to the cytoplasm. 3 / 3
| 1 2

reach
"Our data do not exclude the possibility that, under specific conditions, BAP1 might be exported to the cytoplasm prior to its ubiquitination, although we did not identify any export signal in BAP1."

sparser
"Our data do not exclude the possibility that, under specific conditions, BAP1 might be exported to the cytoplasm prior to its ubiquitination, although we did not identify any export signal in BAP1."

sparser
"The putative E2/E3 hybrid ubiquitin ligase, UBE2O, has been reported to mediate cytoplasmic translocation of BAP1 by multi‐monoubiquitylation of the NLS."
BAP1 translocates to the cytosol. 2 / 2
| 1 1

reach
"Alternatively, UBE2O, which has been reported to suppress its function by recruiting BAP1 to the cytosol, XREF_BIBR is mutated in ESCC and in other esophageal carcinomas (see COSMIC data base)."

sparser
"Alternatively, UBE2O, which has been reported to suppress its function by recruiting BAP1 to the cytosol, xref is mutated in ESCC and in other esophageal carcinomas (see COSMIC data base). xref It is possible that altered UBE2O behaves as an oncoprotein like activated EGFR or RAS family proteins."
BAP1 affects mutations
| 5
BAP1 inhibits mutations. 5 / 5
| 5

sparser
"That being said, it is clear that many metastatic UM cases demonstrate chromosome 3 loss, polysomy 8q, and have BAP1 inactivating mutations."

sparser
"Because poly-(ADP-ribose) polymerase inhibitors (PARPIs) induce synthetic lethality in BRCA1/2 mutant cancers, we evaluated whether BAP1 inactivating mutations confer sensitivity to PARPIs in mesothelioma and if combination therapy with temozolomide (TMZ) is beneficial."

sparser
"BAP1 has inactivating mutations in approximately 47% of primary UMs and 84% of metastatic UMs, consistent with the association of BAP1 mutations with inferior outcomes. xref SF3B1 encodes for splicing factor 3B subunit 1, which is involved in pre-messenger RNA splicing."

sparser
"Moreover, 2 of the 3 KDM1B mutations in LUSC and LUAD are inactivating, and these are also exclusive of BAP1 inactivating mutations, suggesting that KDM1B mutations might play a role in cancer."

sparser
"All carriers of inherited heterozygous germline BAP1 inactivating mutations (BAP1 +/- )developed one and often severalBAP1 -/- malignancies in their lifetime xref , mostly malignant mesothelioma (MM), uveal melanoma (UVM) xref , xref , etc xref - xref ."
BAP1 affects lactose
| 5
BAP1 inhibits lactose. 5 / 5
| 5

reach
"In functional assays, BAP1 was found to inhibit the migration and invasion of LAC cells, and promoted their apoptosis and necrosis."

reach
"Functionally, BAP1 promotes apoptosis and necrosis, and down-regulates the migration and invasion abilities of LAC cells."

reach
"BAP1 may inhibit the progression of LAC by attenuating tumor invasiveness, a function that was supported by the findings of the Transwell assay."

reach
"Finally, to elucidate the mechanism by which BAP1 suppresses invasion and metastasis in LAC cells, we analyzed the expression levels of metastasis related proteins in transfected H1299 cells and H1650 cells using IB, respectively."

reach
"However, BAP1 is closely related to lymph node status, suggesting that BAP1 also mediates suppression of cell migration in LAC which is in keeping with the findings of the wound healing assay."

reach
"In addition to a potentially critical role for BAP1 inactivation in epigenetic deregulation in ccRCC, BAP1 has been found to promote DNA double-strand break repair by homologous recombination, thereby adding to its tumor suppressor function [XREF_BIBR]."

reach
"BAP1 protein was reported to form a complex with host cell factor-1 (HCF-1) and promotes DNA double-strand break repair."

reach
"The literature suggests that BAP1 promotes DNA double-strand break repair and that its loss leads to homologous recombination repair defects, reminiscent of BRCA1/2 deficiency."

reach
"Although BAP1 stimulates DNA double-strand break repair, whether it functions in nucleotide excision repair (NER) is unknown."

reach
"BAP1 loss impairs HR and double-strand break repair, promoting error-prone nonhomologous end joining, with consequent genomic instability (XREF_FIG)."
BAP1 affects TP53
| 5
| 3

sparser
"Concurrent mutations in BAP1 and TP53 were associated with adverse outcome, with similar effects observed for acquired alterations in either of the two genes alone ( xref )."

sparser
"Mutations in BAP1 ( q = 0.004) and TP53 ( q = 0.001) were associated with decreased CSS in a multivariable model; only TP53 ( q = 0.005) remained significant when SSIGN score was included."

sparser
"On analyses adjusted only for cohort, we found that mutations in BAP1 ( n = 1049 for analysis; hazard ratio [HR]: 2.10; 95% confidence interval [CI]: 1.44–3.04; q < 0.001) and TP53 ( n = 784 for analysis; HR: 2.63; 95% CI: 1.36–5.08; q = 0.028) were significantly associated with increased risk of death from cancer after adjustment for multiple comparisons using competing risks regression ( xref )."
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
BAP1 affects SF3B1
| 5
| 3

sparser
"Uveal melanoma (UM) is a relative rare disease and has a high mortality rate due to metastasis in about half of all patients within 15 y after diagnosis. xref , xref , xref It is the most common primary intra‐ocular malignancy in adults in the Western world. xref UM specific mutations in the alpha subunit genes GNAQ and GNA11 are described as well as mutations in BAP1 , SF3B1 , and EIF1AX . xref , xref , xref Mutations in the latter 3 genes are found in ∼75% of all UM and are useful for prognostication of patients. xref , xref , xref BAP1 ‐mutated UM gives rise to early‐onset metastasis whereas SF3B1 ‐mutated UM gives rise to late‐onset metastasis and EIF1AX ‐mutated UM hardly metastasizes. xref Mutations in PLCB4 and CYSLTR2 are described in UM in a mutually exclusive manner to GNAQ or GNA11 mutations but so far have not been associated with prognosis. xref , xref Copy number alterations in chromosomes 1, 3, 6, and 8 are correlated with prognosis of the UM patient. xref , xref UM with EIF1AX , SF3B1 and BAP1 mutations are associated with unique chromosomal patterns, suggesting distinct UM subclasses."

sparser
"The Rotterdam Ocular Melanoma Study Group investigated the association of EIF1AX , SF3B1 and BAP1 mutation with disease-free survival and metastatic risk of patients with uveal melanoma. xref In their recent article, they published a Kaplan-Meier survival probability curve regarding these mutations."

sparser
"Other significantly mutated genes in UM are BAP1 , EIF1AX and SF3B1 , which also form a second mutually‐exclusive subgroup xref (Table  xref )."
| 2

sparser
"To further investigate the genetic interaction between BAP1 and SF3B1 mutations and to exclude that other alterations in Mel202 might contribute to the incompatibility with BAP1 deletion, we asked whether deletion of the SF3B1 ‐mutant allele in Mel202 cells could reverse BAP1 KO‐induced growth arrest."

sparser
"That study highlighted the role of coding and non-coding genes that were differentially expressed between SCNA subtypes of monosomy 3 uveal melanomas that were associated with metastasis. xref Five genes have been reported to be frequently mutated in uveal melanoma. xref Mutations in GNAQ and GNA11 occur early in tumor formation while BAP1 , SF3B1 , and EIF1AX mutations likely occur later in tumor progression. xref Mutation in EIF1AX is an indicator of good prognosis, whereas mutations in SF3B1 and BAP1 are associated with intermediate and poor prognosis."
BAP1 affects RCC
| 5
BAP1 inhibits RCC.
| 3
BAP1 inhibits RCC. 3 / 3
| 3

reach
"Taken together these data show that BAP1 binding to HCF-1 is likely to be important for BAP1 suppression of RCC development."

reach
"XREF_BIBR Immunohistochemistry for BAP1 was performed on tissue microarray sections from 559 non metastatic RCC cases treated with nephrectomy : BAP1 was negative in 82 of 559 tumors (14.7%), and Cox regression indicated a significantly worse disease-free and overall survival for patients negative for BAP1 protein compared to patients with BAP1 positive tumors."

reach
"Thus, BAP1 mutation in the germline may predispose to RCC, in which case, RCC development may also be initiated by loss of BAP1."
BAP1 activates RCC.
| 2
BAP1 activates RCC. 2 / 2
| 2

reach
"Multiple lines of evidence implicate HCF-1 in BAP1 mediated RCC tumor suppression function."

reach
"Here, we report a novel RCC GEMM based on Pax8-Cre deletion of Vhl together with either Bap1 or Pbrm1, and show that Bap1 and Pbrm1 are not only drivers of RCC, but also determinants of tumor grade."
BAP1 affects KDM1B
4 | 1
4 | 1

No evidence text available

sparser
"In addition, the mass spectrometric analysis by xref also identified a physical interaction between BAP1 and the H3K4 demethylase KDM1B (also known as LSD1B), an intriguing interaction that might implicate BAP1/ASXL in KDM1B function and will need to be validated further."

No evidence text available

No evidence text available

No evidence text available
BAP1 affects H3K27me3
| 5
BAP1 decreases the amount of H3K27me3.
| 4
BAP1 decreases the amount of H3K27me3. 2 / 2
| 2

reach
"We next sought to delineate the mechanism by which Bap1 deletion increased H3K27me3 levels."

reach
"Indeed, we observed that loss of BAP1 results in elevated EZH2 expression and increased H3K27me3 levels, and knock-down of Ezh2 leads to decreased H3K27me3 levels in BNC cells (XREF_FIG; and XREF_FIG)."
Modified BAP1 decreases the amount of H3K27me3. 2 / 2
| 2

reach
"Recurrent BAP1 mutations have also been observed in intrahepatic cholangiocarcinoma 25, and a recent study showed that loss of Bap1 in mice increases expression of H3K27me3 and Ezh2 while suppressing expression of PRC2 targets 26."

reach
"Coexpression of ASXL1-MT and BAP1 also reduced bulk H3K27me3 levels, which is probably a direct consequence of loss of H2AK119Ub 24 in 32Dcl3 cells."
BAP1 methylates H3K27me3.
| 1
BAP1 leads to the methylation of H3K27me3 on lysine. 1 / 1
| 1

reach
"BAP1 loss results in increased global trimethylation of histone H3 lysine 27 (H3K27me3), which is catalyzed by the polycomb repressive complex 2 enzyme EZH2."
BAP1 affects H2AC20
3 | 2
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."
BAP1 affects GEP
| 5
BAP1 binds GEP. 3 / 3
| 3

sparser
"Inactivating mutations in the tumor suppressor BAP1 are associated with the class 2 GEP and high metastatic risk xref , whereas single nucleotide substitutions in SF3B1 and EIF1AX are found mainly in class 1 tumors and are associated with intermediate and low metastatic risk, respectively xref , xref ."

sparser
"Mutations in SF3B1 and EIF1AX are associated with Class 1 GEP and have been reported to be good prognostic factors in UM, whereas BAP1 mutations are associated with Class 2 GEP and poor prognosis ( xref , xref )."

sparser
"BAP1 mutations were associated with class 2 GEP ( P < ."
BAP1 binds GEP and class 2. 2 / 2
| 2

sparser
"12,36,39 BAP1 mutation is associated with monosomy 3 or class 2 GEP tumors and impart poor survival."

sparser
"BAP1 is associated with monosomy 3, poor prognosis, and class 2 GEP tumors, while EIF1AX is associated with class 1 GEP tumors and good prognosis."
BAP1 affects CDKN1B
| 5
BAP1 increases the amount of CDKN1B. 5 / 5
| 5

reach
"Depletion of KLF5, BAP1 or HCF-1 also upregulated p27 mRNA levels (XREF_FIG)."

reach
"Knockdown of either KLF5 or BAP1 upregulated p27 protein levels in these breast cell lines as well (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"Indeed, depletion of KLF5, BAP1, HCF-1 or OGT upregulated p27 protein expression and blocked cell cycle progression (XREF_FIG)."

reach
"Given that depletion of BAP1 or KLF5 markedly increased p27 protein levels, we speculated that the protein complex might regulate the expression of p27 and FGF-BP (a KLF5 target gene)."

reach
"Knockdown of KLF5, BAP1 or HCF-1, but not OGT, significantly increased p27 mRNA levels (XREF_FIG)."
ASXL proteins affects BAP1
| 5
ASXL proteins binds BAP1.
| 3
BAP1 binds ASXL proteins. 3 / 3
| 3

reach
"The ASXL proteins directly interact with BAP1, KDM1A, NHRs and NCOA1 to target specific genomic loci using histone modifications."

reach
"The interaction between BAP1 and the ASXL proteins is mediated by the ~ 120-residue DEUBAD (DEUBiquitinase ADaptor) domain of the ASXL proteins [XREF_BIBR], and this region is sufficient for activation of BAP1 in in vitro H2AK119ub1 nucleosome deubiquitination assays [XREF_BIBR]."

reach
"ASXL proteins interact with BAP1 to form the polycomb repressive deubiquitinase (PR-DUB) complex, which removes monoubiquitin from histone 2A at lysine 119 (H2AK119) [XREF_BIBR, XREF_BIBR]."
ASXL proteins activates BAP1.
| 2
ASXL proteins activates BAP1. 2 / 2
| 2

reach
"ASXL proteins activate BAP1 by forming the polycomb repressive deubiquitinase (PR-DUB) complex which acts on H2AK119ub1."

reach
"ASXL proteins activate BAP1 by forming the polycomb repressive deubiquitinase (PR-DUB) complex which acts on H2AK119ub1."
| PMC
3p21.1 losses affects BAP1
| 5
3p21.1 losses activates BAP1. 5 / 5
| 5

reach
"The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma."

reach
"The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma."

reach
"The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma."

reach
"The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma."

reach
"The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma."
Ab affects ULD
| 4
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
VHL affects SETD2
| 4
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
ULD affects ab
| 4
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
SETD2 affects VHL
| 4
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
| 4

reach
"It has recently been reported that the RING finger domain of the Breast and Ovarian Cancer Susceptibility Gene product, BCRA-1, binds to the nuclear localized ubiquitin carboxy-terminal hydrolase BAP1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"BAP1 was originally found to interact with the RING finger domain of BRCA1 and to perform the cell growth-suppressive function."

reach
"BAP1 interacts with the BRCA1 RING finger domain, which mediates BRCA1 's E3 ubiquitin ligase activity [XREF_BIBR]."

reach
"BAP1 direct physical interaction with BRCA1-RING finger domain was associated to enhancement of BRCA1 tumour suppressor activity in breast cancer [11] (Fig. 1a)."
PRC2_complex affects BAP1
| 1 3

sparser
"In addition, BAP1 mutations in ccRCC tumors were associated with downregulated expression of target genes of polycomb repressive complex 2 (PRC2) [ xref ] ( xref )."

sparser
"Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells."

sparser
"BAP1 does not interact with PRC2 proteins ( xref )."

reach
"Although PR-DUB previously was shown to cooperate with PRC2, we observed minimal overlap and functional interaction between BAP1 and PRC2 in embryonic stem cells."
PDH affects BAP1
| 4
PDH activates mutated BAP1. 4 / 4
| 4

reach
"Moreover, we shown that inhibition of PDH phosphorylation reduces BAP1 mutant UM cell growth by induction of cell cycle arrest."

reach
"Our results suggest that PDH inactivation causes increased glucose utilization in BAP1 mutant UM."

reach
"Suppression of PDH phosphorylation significantly decreases UM cell growth and glycolytic abilities of BAP1 mutant UM cells, but not BAP1 wild type UM cells."

reach
"These results indicate that inhibition of PDH phosphorylation by PDHK1 inhibitions decreases BAP1 mutant UM cell growth via cell cycle arrest."
PBRM1 affects VHL
| 4
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
MP65 affects BAP1
| 4
BAP1 binds MP65. 4 / 4
| 4

sparser
"In addition, these inhibitors did not alter MP65 and MP65-BAP1 growth ( xref )."

sparser
"Both inhibitors diminished MP65 and MP65-BAP1 growth; however, MP65 were more sensitive to these compounds compared to MP65-BAP1 ( xref )."

sparser
"Since we have demonstrated MP65 have higher glycolysis, glycolytic capacity and increased nucleotide biosynthesis metabolites compared to MP65-BAP1, we examined key enzymes involved in glycolysis and the PPP."

sparser
"We found that MP65 (OXPHOS high ) were more resistant to both metabolic stress environments than MP65-BAP1, whereas MP46 (OXPHOS low ) were more vulnerable to stressful conditions compared to MP46-BAP1 ( xref and xref )."
HIF1A affects BAP1
| 1 3
| 1 3

sparser
"We demonstrated that during hypoxia, BAP1 binds, deubiquitylates, and stabilizes HIF-1α, the master regulator of the hypoxia response and tumor cell invasion."

sparser
"We found that BAP1 binds to the N-terminal region of HIF-1α, where HIF-1α binds DNA and dimerizes with HIF-1β forming the heterodimeric transactivating complex HIF."

reach
"HIF1alpha-dependent metabolism:. Bioinformatics interrogation of The Cancer Genome Atlas (TCGA) data have associated BAP1 alterations with changes in hypoxia-inducible factor-1 alpha (HIFα) expression (25,76,77)."

sparser
"In addition, we observed that hypoxic conditions increase exogenous BAP1 expression, raising the possibility of future studies to investigate HIF1α-BAP1 cross-talk, and its role in metabolic alterations."
HDAC affects BAP1
| 4
HDAC inhibits BAP1. 4 / 4
| 4

reach
"We then investigated whether HDAC inhibition could reverse the phenotypic consequences of BAP1 loss : loss of melanocytic differentiation and acquisition of the class 2 gene expression profile."

reach
"Histone deacetylase (HDAC) inhibitors have been shown to reverse the phenotypic effects of BAP1 loss by inducing morphologic differentiation and transition from a high-risk to a low-risk gene expression profile in uveal melanoma cells."

reach
"The Bap1 deficient phenotype can be rescued with human BAP1, by pharmacologic inhibition of histone deacetylase (HDAC) activity or by specific knockdown of Hdac4."

reach
"Histone deacetylase (HDAC) inhibitors, such as the readily available valproic acid, can reverse this effect of BAP1 loss and may play a role in treated patients with high-risk class 2 uveal melanomas in an adjuvant setting prior to the emergence of overt metastatic disease [XREF_BIBR]."
EED affects ASXL1
| 4
ASXL1 binds EED and BAP1. 4 / 4
| 4

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

sparser
"BAP1 interacts with ASXL1 polycomb chromatin binding protein forming a polycomb repressive deubiquitinase complex that regulates histone H2A ubiquitination xref ."

sparser
"BAP1 also interacts with ASXL1 to form the Polycomb group repressive deubiquitinase complex (PR-DUB), which is involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"8 BAP1 interacts with ASXL1 (MIM 612990 ) to form the Polycomb group Repressive Deubiquitinase complex (PR-DUB)."
COPA affects BAP1
| 2 2
| 2 2

sparser
"We note that only a small fraction of total COPA binds to BAP1 as input levels are much higher than COPA levels in the coIP, correlating with the stoichiometry observed in the AP-MS experiment ( xref and xref )."
| PMC

reach
"These data confirm the interaction between BAP1 and COPA on endogenous protein and indicate that this interaction is not due to overexpression artefacts."
| PMC

reach
"In all BAP1 IP samples, but not the negative control normal IgG samples endogenous COPA coimmunoprecipitates with endogenous BAP1.We note that only a small fraction of total COPA binds to BAP1 as input levels are much higher than COPA levels in the coIP, correlating with the stoichiometry observed in the AP-MS experiment (Fig 5A and S2 File)."
| PMC

sparser
"These data confirm the interaction between BAP1 and COPA on endogenous protein and indicate that this interaction is not due to overexpression artefacts."
| PMC
BAP1 affects radioresistance
| 4
BAP1 activates radioresistance. 4 / 4
| 4

eidos
"BAP1 was found to mediate radioresistance in an in vivo xenograft model and HNSCC cell lines via the deubiquitination of H2A and modulation of HR ."

eidos
"In conclusion , we found that BAP1 can deubiquitinate H2Aub and induces radioresistance in HNSCC cells via modulation of HR ."

eidos
"Thus , BAP1 seems to regulate H2Aub in HNSCC cells and may be a mechanism by which BAP1 induces radioresistance via facilitating DNA repair ."

eidos
"We observed that 2 Gy x 5 days of radiation treatment had minimal effect on control tumors ( Fig. 5 ) but led to a significant decrease in growth of BAP1-KD tumors ( Fig. 5 ; P < 0.05 ) , suggesting that BAP1 mediated radioresistance in this xenograft model ."
| 4
| 2

reach
"XREF_FIG shows that BAP1 +/- fibroblasts transduced with AdBAP1 had reduced glycolysis and glycolytic capacity; in contrast, AdC91S was ineffective at reducing glycolysis (rate of glycolysis in BAP1 +/- transduced with : AdGFP 24.94 +/-1.77 mpH and min, AdBAP1 6.92 +/-0.24 mpH and min, AdBAP1 (C91S) 20.33 +/-2.92 mpH and min; glycolytic capacity : AdGFP 30.33 +/-1.71 mpH and min, AdBAP1 11.50 +/-0.43 mpH and min, AdC91S 25.08 +/-3.25 mpH and min)."

reach
"Additionally, reduced levels of BAP1 induce aerobic glycolysis, which favors tumor growth (64)."
| 2
Mutated BAP1 activates glycolytic process. 2 / 2
| 2

reach
"These data suggest that BAP1 mutant UM cells have increased aerobic glycolysis compared to BAP1 wild type UM cells."

reach
"For example, mesothelioma cells that carry germline BAP1 mutations have upregulated aerobic glycolysis (17), and BAP1 mutant ccRCC tumor samples display elevated glycolysis (22)."
BAP1 affects gemcitabine
| 4
| 4

reach
"BAP1 modulates sensitivity to gemcitabine in CCA cells."

reach
"BAP1 dependent expression of long non coding RNA NEAT-1 contributes to sensitivity to gemcitabine in cholangiocarcinoma."

reach
"Taken together these studies indicate that BAP1 expression modulates sensitivity to gemcitabine."

reach
"Parasramka et al [158] found that BAP1 expression could modulate CCA cell sensitivity to gemcitabine, or more specifically, sensitivity to gemcitabine was increased in low BAP1 expressing CCA cells than in high BAP1 expressing CCA cells."
BAP1 affects angiogenesis
| 1 3
| 1 3

reach
"P-I metalloproteinases and LAAO from Bothrops spp. inhibit angiogenesis ."

eidos
"In vivo results using a chicken embryo choriollantoic membrane ( CAM ) assay showed that BaP1 inhibits tumor growth , angiogenesis , and tumor proliferation ."

reach
"In vivo results using a chicken embryo choriollantoic membrane (CAM) assay showed that BaP1 inhibits tumor growth, angiogenesis, and tumor proliferation."

reach
"P-I metalloproteinases and L-amino acid oxidases from Bothrops species inhibit angiogenesis."
BAP1 affects ab
| 4
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
BAP1 affects TUBG
| 4
BAP1 deubiquitinates TUBG. 4 / 4
| 4

reach
"A recent study has linked the deubiquitination of gamma-tubulin by BAP1 to breast cancer."

reach
"While the mechanism remains to be fully elucidated, it seems that deubiquitylation of gamma-tubulin by BAP1 during mitosis allows proper spindle organisation and function [XREF_BIBR]."

reach
"Another study showed de-ubiquitination of gamma-tubulin by BAP1 prevents chromosome instability in breast cancer cells [15]."

reach
"Deubiquitination of gamma-tubulin by BAP1 prevents chromosome instability in breast cancer cells."
BAP1 affects TNMD
| 1 3
BAP1 inhibits TNMD.
| 1 1
BAP1 inhibits TNMD. 2 / 2
| 1 1

eidos
"These results suggest that BAP1 expression favors activation of the RhoA pathway over the Rac1 pathway , such that loss of BAP1 shifts the balance toward Rac1-driven motility and increased TEM ."

reach
"We find that this process requires VCAM mediated adhesion between UM cells and ECs and that loss of the metastasis suppressor BAP1 enhances TEM."
BAP1 activates TNMD.
| 2
BAP1 activates TNMD. 2 / 2
| 2

reach
"As predicted, shRNA mediated depletion of BAP1 increased the overall rate of TEM by UM cells in transwell assays."

reach
"BAP1 Depletion Enhances TEM."
| 4

reach
"It has recently been reported that the RING finger domain of the Breast and Ovarian Cancer Susceptibility Gene product, BCRA-1, binds to the nuclear localized ubiquitin carboxy-terminal hydrolase BAP1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"BAP1 was originally found to interact with the RING finger domain of BRCA1 and to perform the cell growth-suppressive function."

reach
"BAP1 interacts with the BRCA1 RING finger domain, which mediates BRCA1 's E3 ubiquitin ligase activity [XREF_BIBR]."

reach
"BAP1 direct physical interaction with BRCA1-RING finger domain was associated to enhancement of BRCA1 tumour suppressor activity in breast cancer [11] (Fig. 1a)."
BAP1 affects PARP3
| 4
| 4

sparser
"Thus, we suspect that the novel interaction of BAP1 and PARP3 may also be perturbed by PARP inhibitors, leading to inhibition of cancer growth."

sparser
"In this scenario, it is possible that the novel interaction of BAP1 with PARP3 in cancerous cells may be promoting cancerous growth, possibly through regulation of DNA repair and apoptosis."

sparser
"Several studies and clinical trials [ xref ], have shown that PARP inhibitors influence cancers in which mutations in BRCA1 or BRCA2 are observed, which led us to assume that the cancerous growth-inhibiting interaction of BAP1 with BRCA1 may already be perturbed in this case, and that PARP inhibitors may actually be blocking the novel interaction of BAP1 with PARP3 which enhances cancer growth."

sparser
"Perturbation of the interaction of BAP1 with PARP3 , using PARP3 inhibitors, may then suppress cancerous growth, at least in sarcomatoid MPM."
BAP1 affects MP65
| 4
BAP1 binds MP65. 4 / 4
| 4

sparser
"In addition, these inhibitors did not alter MP65 and MP65-BAP1 growth ( xref )."

sparser
"Both inhibitors diminished MP65 and MP65-BAP1 growth; however, MP65 were more sensitive to these compounds compared to MP65-BAP1 ( xref )."

sparser
"Since we have demonstrated MP65 have higher glycolysis, glycolytic capacity and increased nucleotide biosynthesis metabolites compared to MP65-BAP1, we examined key enzymes involved in glycolysis and the PPP."

sparser
"We found that MP65 (OXPHOS high ) were more resistant to both metabolic stress environments than MP65-BAP1, whereas MP46 (OXPHOS low ) were more vulnerable to stressful conditions compared to MP46-BAP1 ( xref and xref )."
BAP1 affects H2AK119ub
| 4
BAP1 activates H2AK119ub. 4 / 4
| 4

reach
"32Dcl3 cells expressing ASXL1-MT showed global reduction of H2AK119ub, which was dramatically enhanced by the coexpression of BAP1, but not by that of BAP1-C91S."

reach
"Moreover, BAP1 depletion also abrogated the global reduction of H2AK119ub induced by ASXL1-MT."

reach
"In line with our results using murine 32Dcl3 cells, ASXL1-MT was associated with global reduction of H2AK119ub, which was dramatically enhanced by the coexpression of BAP1."

reach
"Expression of ASXL1-WT showed no effect on global reduction of H2AK119ub, which was only slightly enhanced by the coexpression of BAP1."
BAP1 affects ERK
| 4
BAP1 inhibits ERK.
| 2
BAP1 inhibits ERK. 2 / 2
| 2

reach
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways."

reach
"Taken together, our data suggested that BAP1 negatively regulated the ERK1/2 and JNK/c-Jun signaling pathways to exert its tumor-suppressive functions in ICC."
BAP1 dephosphorylates ERK.
| 2
BAP1 leads to the dephosphorylation of ERK. 1 / 1
| 1

reach
"More importantly, our results also confirmed that BAP1 deficiency increased the phosphorylation and activity of ERK1/2 and JNK/c-Jun signaling to promote ICC progression."
Mutated BAP1 leads to the dephosphorylation of ERK. 1 / 1
| 1

reach
"As expected, we indeed found that phosphorylation of ERK1/2 and JNK/c-Jun was inhibited by wild-type but not mutant BAP1 in ICC, as shown by western blotting and immunohistochemistry."
BAP1 affects DEUBAD
| 4
BAP1 leads to the ubiquitination of DEUBAD. 2 / 2
| 2

reach
"BAP1 promotes DEUBAD monoubiquitination resulting in an increased stability of ASXL2, which in turn stimulates BAP1 DUB activity."

reach
"However, monoubiquitination of DEUBAD was induced to a similar extent by WT BAP1 or a catalytically inactive (C91S) mutant (Fig. 2d)."
BAP1 leads to the ubiquitination of DEUBAD on K325. 1 / 1
| 1

reach
"Interestingly, we also found that overexpression of BAP1 or Calypso in HEK293T cells promoted the monoubiquitination of the DEUBAD of Asx and ASXL2 on K325 and K370, respectively (Supplementary Fig. 2c, d)."
BAP1 leads to the ubiquitination of DEUBAD on K370. 1 / 1
| 1

reach
"Interestingly, we also found that overexpression of BAP1 or Calypso in HEK293T cells promoted the monoubiquitination of the DEUBAD of Asx and ASXL2 on K325 and K370, respectively (Supplementary Fig. 2c, d)."
BAP1 affects COPA
| 2 2
| 2 2

sparser
"We note that only a small fraction of total COPA binds to BAP1 as input levels are much higher than COPA levels in the coIP, correlating with the stoichiometry observed in the AP-MS experiment ( xref and xref )."
| PMC

reach
"These data confirm the interaction between BAP1 and COPA on endogenous protein and indicate that this interaction is not due to overexpression artefacts."
| PMC

reach
"In all BAP1 IP samples, but not the negative control normal IgG samples endogenous COPA coimmunoprecipitates with endogenous BAP1.We note that only a small fraction of total COPA binds to BAP1 as input levels are much higher than COPA levels in the coIP, correlating with the stoichiometry observed in the AP-MS experiment (Fig 5A and S2 File)."
| PMC

sparser
"These data confirm the interaction between BAP1 and COPA on endogenous protein and indicate that this interaction is not due to overexpression artefacts."
| PMC
BAP1 affects ASXL1/2
| 3 1
BAP1 binds ASXL1/2. 4 / 4
| 3 1

reach
"The tumor suppressor BAP1 associates with ASXL1/2 to form the core Polycomb complex PR and DUB, which catalyzes the removal of mono-ubiquitin from several substrates including histone H2A."

reach
"The core BAP1 and ASXL1/2 complex interacts with host cell factor-1 (HCF-1) and histone modifying complexes during cell division."

sparser
"This work provides a structural basis upon which to interpret previous studies showing that the ASXL1/2 Deubad domains bind tightly to BAP1, and activate the complex by forming a composite binding site for ubiquitin xref , xref ."

reach
"Cancer-associated BAP1 mutations lead loss of DUB activity and impair function of the BAP1-ASXL1/2 complex, causing an elevation in H2A ubiquitination levels, and subsequent cell cycle progression dysregulation (3)."
ASXL2 affects ab
| 4
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
ASXL1/2 affects BAP1
| 3 1
BAP1 binds ASXL1/2. 4 / 4
| 3 1

reach
"The tumor suppressor BAP1 associates with ASXL1/2 to form the core Polycomb complex PR and DUB, which catalyzes the removal of mono-ubiquitin from several substrates including histone H2A."

reach
"The core BAP1 and ASXL1/2 complex interacts with host cell factor-1 (HCF-1) and histone modifying complexes during cell division."

sparser
"This work provides a structural basis upon which to interpret previous studies showing that the ASXL1/2 Deubad domains bind tightly to BAP1, and activate the complex by forming a composite binding site for ubiquitin xref , xref ."

reach
"Cancer-associated BAP1 mutations lead loss of DUB activity and impair function of the BAP1-ASXL1/2 complex, causing an elevation in H2A ubiquitination levels, and subsequent cell cycle progression dysregulation (3)."
ASXL1 affects EED
| 4
ASXL1 binds EED and BAP1. 4 / 4
| 4

sparser
"In flies and humans, the Polycomb repressive deubiquitinase (PR-DUB) complex is formed through interactions of BAP1 and ASXL1 [ xref ]."

sparser
"BAP1 interacts with ASXL1 polycomb chromatin binding protein forming a polycomb repressive deubiquitinase complex that regulates histone H2A ubiquitination xref ."

sparser
"BAP1 also interacts with ASXL1 to form the Polycomb group repressive deubiquitinase complex (PR-DUB), which is involved in stem cell pluripotency and other developmental processes ( xref , xref )."

sparser
"8 BAP1 interacts with ASXL1 (MIM 612990 ) to form the Polycomb group Repressive Deubiquitinase complex (PR-DUB)."
| 2

reach
"We show that the ubiquitin conjugating enzyme UBE2O multi-monoubiquitinates the nuclear localization signal of BAP1, thereby inducing its cytoplasmic sequestration."

reach
"It was recently shown that the ubiquitin conjugating enzyme UBE2O multi-monoubiquitinates tumor suppressor BAP1 on its NLS bp to promote cytoplasmic localization."

reach
"The ubiquitin conjugating enzyme UBE2O induces BAP1 sequestration in the cytoplasm by multi monoubiquitylation of its nuclear localization signal (NLS)."

reach
"BAP1 binds and deubiquitinates the transcriptional regulator host cell factor 1 (HCF-1), which interacts with histone modifying complexes [8,9]."

reach
"Although BAP1 interacts with a transcriptional regulator, HCF-1, and transcription factors FoxK1 and FoxK2, how BAP1 controls gene expression remains unclear."

reach
"In fact, BAP1 binds and deubiquitinates the transcriptional regulator host cell factor 1 that interacts with histone modifying complexes [19]."
| 3
Glycolic acid inhibits BAP1.
| 2
| 2

reach
"Glycolic acid inhibited the proteolytic activity of BaP1 on azocasein, with an IC50 of 1.67 mM."

reach
"In fact, a recent study showed that glycolic acid, a potent antioxidant and metal chelator agent, was able to inhibit the enzymatic and biological effects of the SVMP BaP1, a metalloproteinase isolated from Bothrops asper snake venom XREF_BIBR."
| 1

reach
"Results of these assays suggest that glycolic acid interacts directly with BaP1 and chelates the Zn (2) + ion at the active site."
VPS affects BAP1
| 1 2
| 1 2

sparser
"Next, we investigated how the RbmC and Bap1 matrix proteins interact with VPS during biofilm formation."

reach
"These observations indicate that Bap1 and RbmC could bind VPS, and these interactions may influence the spatial localization of these matrix proteins."

sparser
"These observations indicate that Bap1 and RbmC could bind VPS, and these interactions may influence the spatial localization of these matrix proteins."
SF3B1 affects EIF1AX
| 3
| 3

sparser
"Uveal melanoma (UM) is a relative rare disease and has a high mortality rate due to metastasis in about half of all patients within 15 y after diagnosis. xref , xref , xref It is the most common primary intra‐ocular malignancy in adults in the Western world. xref UM specific mutations in the alpha subunit genes GNAQ and GNA11 are described as well as mutations in BAP1 , SF3B1 , and EIF1AX . xref , xref , xref Mutations in the latter 3 genes are found in ∼75% of all UM and are useful for prognostication of patients. xref , xref , xref BAP1 ‐mutated UM gives rise to early‐onset metastasis whereas SF3B1 ‐mutated UM gives rise to late‐onset metastasis and EIF1AX ‐mutated UM hardly metastasizes. xref Mutations in PLCB4 and CYSLTR2 are described in UM in a mutually exclusive manner to GNAQ or GNA11 mutations but so far have not been associated with prognosis. xref , xref Copy number alterations in chromosomes 1, 3, 6, and 8 are correlated with prognosis of the UM patient. xref , xref UM with EIF1AX , SF3B1 and BAP1 mutations are associated with unique chromosomal patterns, suggesting distinct UM subclasses."

sparser
"The Rotterdam Ocular Melanoma Study Group investigated the association of EIF1AX , SF3B1 and BAP1 mutation with disease-free survival and metastatic risk of patients with uveal melanoma. xref In their recent article, they published a Kaplan-Meier survival probability curve regarding these mutations."

sparser
"Other significantly mutated genes in UM are BAP1 , EIF1AX and SF3B1 , which also form a second mutually‐exclusive subgroup xref (Table  xref )."
PSME3 affects BAP1
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
PPARGC1A affects BAP1
| 2 1
| 2 1

reach
"PUGNAc treatment promotes the interaction between PGC-1alpha and BAP1, while it has no effect on the S333A mutant (XREF_FIG), suggesting that O GlcNAcylation of PGC-1alpha facilitates BAP1 binding."

reach
"38 Furthermore, the authors found that high-glucose conditions potentiated PGC1alpha glycosylation, suggesting that this O-GlcNAc-induced PGC1alpha and BAP1 complex may provide a functional explanation for the aberrant induction of hepatic gluconeogenesis in hyperglycemic patients."

sparser
"Conversely, O -GlcNAcylation of the transcriptional regulator PGC1α facilitates the binding of the deubiquitinase BAP1 to PGC1α, leading thus to its stabilization by blocking its ubiquitination ( xref )."
OCM affects BAP1
| 3
| 2

sparser
"These findings suggest that BAP1 alterations may be associated with a more aggressive OM phenotype."

sparser
"Germline BAP1 mutations are associated with a more aggressive OM phenotype and a recurrent phenotypic complex of c utaneous/ o cular m elanoma, atypical m elanocytic proliferations and o ther internal n eoplasms (ie."
OCM binds BAP1 and CM. 1 / 1
| 1

sparser
"In conclusion, our evidence suggests that germline BAP1 inactivation is preferentially associated with metastatic OM and hereditary CM-OM in a small but significant proportion of cases."
NUMB affects BAP1
| 3
| 3

sparser
"Third, similar to N1ICD, NUMB could bind to BAP1 N-terminal or C-terminal part ( xref )."

sparser
"First, both exogenously expressed and endogenous BAP1 could bind to endogenous NUMB ( xref )."

sparser
"We next examined whether BAP1 binds to NUMB and whether this association regulates N1ICD stability."
NCIT:C94600 affects BAP1
| 3
| 3

sparser
"Therefore, our results strongly suggest that BCR-ABL-induced inhibition of BAP1 (at mRNA and protein levels) is a tyrosine kinase-dependent event."

sparser
"In conclusion, the present work points out the significance of BAP1 inhibition by BCR-ABL in the CML."

sparser
"We next asked whether the inhibition of BAP1 by BCR-ABL was dependent on the tyrosine kinase activity of the latter."
MYB affects BAP1
3 |
MYB decreases the amount of BAP1. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
HDAC inhibitors affects BAP1
| 3
HDAC inhibitors inhibits BAP1. 3 / 3
| 3

reach
"XREF_BIBR, XREF_BIBR Moreover, HDAC inhibitors, such as valproic acid, reverse the effect of BAP1 loss in UM and inhibit tumor metastasis XREF_BIBR, XREF_BIBR (XREF_TABLE)."

reach
"Indeed, BAP1 might regulate the expression of histone deacetylases (HDACs), suggesting a potential window of opportunity for clinically available HDAC inhibitors targeting BAP1 mutated tumors [37]."

reach
"However, in short term cultures, HDAC inhibitors did indeed reverse the biochemical, transcriptomic and morphologic consequences of BAP1 loss in primary UM cells."
HAT1 affects BAP1
2 | 1
2 | 1

No evidence text available

reach
"HAT1 and COPI interaction with BAP1 is observed in the cytoplasm and not in the nuclear fractions (Figs 1A and 1B and 4)."
| PMC

No evidence text available
EIF1AX affects SF3B1
| 3
| 3

sparser
"Uveal melanoma (UM) is a relative rare disease and has a high mortality rate due to metastasis in about half of all patients within 15 y after diagnosis. xref , xref , xref It is the most common primary intra‐ocular malignancy in adults in the Western world. xref UM specific mutations in the alpha subunit genes GNAQ and GNA11 are described as well as mutations in BAP1 , SF3B1 , and EIF1AX . xref , xref , xref Mutations in the latter 3 genes are found in ∼75% of all UM and are useful for prognostication of patients. xref , xref , xref BAP1 ‐mutated UM gives rise to early‐onset metastasis whereas SF3B1 ‐mutated UM gives rise to late‐onset metastasis and EIF1AX ‐mutated UM hardly metastasizes. xref Mutations in PLCB4 and CYSLTR2 are described in UM in a mutually exclusive manner to GNAQ or GNA11 mutations but so far have not been associated with prognosis. xref , xref Copy number alterations in chromosomes 1, 3, 6, and 8 are correlated with prognosis of the UM patient. xref , xref UM with EIF1AX , SF3B1 and BAP1 mutations are associated with unique chromosomal patterns, suggesting distinct UM subclasses."

sparser
"The Rotterdam Ocular Melanoma Study Group investigated the association of EIF1AX , SF3B1 and BAP1 mutation with disease-free survival and metastatic risk of patients with uveal melanoma. xref In their recent article, they published a Kaplan-Meier survival probability curve regarding these mutations."

sparser
"Other significantly mutated genes in UM are BAP1 , EIF1AX and SF3B1 , which also form a second mutually‐exclusive subgroup xref (Table  xref )."
| 3

sparser
"They found that BAP1 did not appear to be involved in the deubiquitination of the BRCA1/BARD1 complex,29 and BAP1-mediated growth suppression in vitro was shown to be independent of wild-type BRCA1.4 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"According to this hypothesis, Nishikawa et al showed that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of the BRCA1/BARD1 complex, and that BAP1 and BRCA1/BARD1 mutually regulate ubiquitination in the cell cycle and in the DNA damage response pathway. xref According to the present state of knowledge, it is reasonable to speculate that BAP1 may exert a regulatory role in the DNA damage response."

sparser
"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1/BARD1."
E2F1 affects BAP1
| 1 2
| 1 2

sparser
"Furthermore, in chromatin immunoprecipitation experiments, BAP1 could bind to E2F1 responsive promoters and the localization of BAP1 to E2F1-responsive promoters is host cell factor-1 dependent."

reach
"BAP1 binds to E2F1-responsive gene promoters through HCF-1 and upregulates their expression, and BAP1 KO inhibits the growth of uveal melanoma cells ."

sparser
"Pan et al. have indicated that BAP1 may interact with promoters regulated by E2F1 and affect the cell cycle progression genes controlled by E2F. xref "
E2F affects BAP1
| 3
| 3

sparser
"14 At present, it is not known whether BAP1 directly interacts with ubiquitinated E2F transcription factors."

sparser
"In addition, co-immunoprecipitation experiments have shown that BAP1 directly associates with E2F family members, suggesting a possible additional direct control of these transcriptional factors by BAP1 xref ."

sparser
"Presently, however, direct evidence of BAP1 interacting with ubiquitinated E2F transcription factors is still lacking."
COPI complex affects BAP1
| 3
BAP1 binds COPI complex. 3 / 3
| 3

reach
"Overall, the loss of mass of COPA on blot could not be tied to the loss of ubiquitin and additional experiments are required to explain this observation.To exclude that the BAP1 interaction with the COPI complex is an artifact of overexpression of the exogenous BAP1 construct, we immunoprecipitated endogenous BAP1 using different antibodies against BAP1 in cell lysate from HeLa FRT parental cells (Fig 5D)."
| PMC

reach
"It was intriguing that BAP1 has such a sequence in its C-terminus, but our mutational analysis suggests that the interaction between BAP1 and the COPI complex is not mediated through the C-terminal KxKxx sequence in BAP1."
| PMC

reach
"AP-immunoblotting of GFP-COPE shows interaction of COPA as expected, however BAP1 cannot be observed which is probably due to the low levels of BAP1 that bind to the COPI complex (Fig 5E)."
| PMC
CAMP affects BAP1
| 3
| 3

sparser
"However, the estimated charge of the Bap1 protein monomer is −12.9 at pH 7.0 (), providing a possible explanation for the binding of the LL-37 peptide to the negatively charged target protein, the Bap1 protein, by an electrostatic interaction."

sparser
"We suggest that due to PmB treatment of the bacterial strain, the OmpT LDV residues become exposed during biogenesis on the surface of the OMVs, allowing for binding of the Bap1 protein and thereby promoting LL-37 binding to Bap1."

sparser
"The mechanism(s) of interaction between the LL-37 and the Bap1 protein is under investigation in our laboratory."
BIRC5 affects BAP1
| 1 2
| 1 2

reach
"Furthermore, co-immunoprecipitation experiments were done, but did not demonstrate direct protein protein interaction between BAP1 and survivin (data not shown)."

sparser
"Furthermore, co-immunoprecipitation experiments were done, but did not demonstrate direct protein-protein interaction between BAP1 and survivin (data not shown)."

sparser
"In untreated A375 and C918 cells, the loss of BAP1 was associated with a dramatic reduction in survivin levels ( xref , control “C” lanes), consistent with the prior analysis."
| 3

sparser
"They found that BAP1 did not appear to be involved in the deubiquitination of the BRCA1/BARD1 complex,29 and BAP1-mediated growth suppression in vitro was shown to be independent of wild-type BRCA1.4 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"According to this hypothesis, Nishikawa et al showed that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of the BRCA1/BARD1 complex, and that BAP1 and BRCA1/BARD1 mutually regulate ubiquitination in the cell cycle and in the DNA damage response pathway. xref According to the present state of knowledge, it is reasonable to speculate that BAP1 may exert a regulatory role in the DNA damage response."

sparser
"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1/BARD1."

reach
"BAP1 binds and deubiquitinates the transcriptional regulator host cell factor 1 (HCF-1), which interacts with histone modifying complexes [8,9]."

reach
"Although BAP1 interacts with a transcriptional regulator, HCF-1, and transcription factors FoxK1 and FoxK2, how BAP1 controls gene expression remains unclear."

reach
"In fact, BAP1 binds and deubiquitinates the transcriptional regulator host cell factor 1 that interacts with histone modifying complexes [19]."
BAP1 affects mTORC1
| 1 2
BAP1 inhibits mTORC1.
| 1 1
BAP1 inhibits mTORC1. 2 / 2
| 1 1

eidos
"Nonetheless , the mechanisms underlying how mTORC1 is activated by BAP1 loss and whether BAP1 mutation truly imparts enhanced sensitivity to mTOR inhibition remain unclear ."

sparser
"Interestingly, there is a correlation between BAP1 inactivation and activation of mTORC1. xref , xref However, whether BAP1 loss correlates with sensitivity with mTORC1 inhibitors remains to be established."
BAP1 binds mTORC1.
| 1
| 1

sparser
"PBRM1 and BAP1 likely affect different epigenetic programs, and BAP1 loss is associated with high grade and mTORC1 activation."
BAP1 affects localization
| 3
| 3

reach
"For example, UBE2O binds to and multi-monoubiquitinates BAP1 to induce its cytoplasmic localization and promotes adipocyte differentiation [XREF_BIBR]."

reach
"It was recently shown that the ubiquitin conjugating enzyme UBE2O multi-monoubiquitinates tumor suppressor BAP1 on its NLS bp to promote cytoplasmic localization."

reach
"Nuclear BAP1 is also associated in chromatin remodeling (49), DNA double-strand repair (50,51), and auto-deubiquitylation promoting its own nuclear localization (52)."
BAP1 affects laminin
| 3
BAP1 inhibits laminin. 3 / 3
| 3

reach
"However, BaP1 was able to degrade laminin in PCV, evidencing its ability to reach the BM of this vessel type."

reach
"The ability of BaP1 to reduce the immunostaining of laminin to a greater extent than type IV collagen might be due to the fact that type IV collagen constitutes a more stable covalently linked network, in contrast to laminin [XREF_BIBR - XREF_BIBR]."

reach
"In the ex vivo model, BaP1 was able to degrade type IV collagen and laminin from the BM of microvessels."

reach
"These results highlight a mechanism by which BaP1 induces PGE biosynthesis and suggest that activation of mPGES-1 gene expression is the primary mechanism leading to an increase in this protein."

reach
"As activation of this receptor by PGE can induce synthesis of other inflammatory proteins, including COX-2 and mPGES-1, we investigated the involvement of EP4 in BaP1-induced effects."
Mutated BAP1 activates biosynthetic process. 1 / 1
| 1

reach
"We identified two distinct metabolic phenotypes in BAP1 mutant UM cells, which have either increased glycolysis-nucleotide biosynthesis or FAO."
BAP1 affects VPS
| 1 2
| 1 2

sparser
"Next, we investigated how the RbmC and Bap1 matrix proteins interact with VPS during biofilm formation."

reach
"These observations indicate that Bap1 and RbmC could bind VPS, and these interactions may influence the spatial localization of these matrix proteins."

sparser
"These observations indicate that Bap1 and RbmC could bind VPS, and these interactions may influence the spatial localization of these matrix proteins."
BAP1 affects SP1
| 3
BAP1 activates SP1.
| 2
BAP1 activates SP1. 2 / 2
| 2

reach
"BAP1 phosphorylation-mediated Sp1 stabilization plays a critical role in cathepsin K inhibition-induced C-terminal p53-dependent Bax upregulation."

reach
"Mitochondrial ROS scavengers prevented DNA damage, BAP1-mediated Sp1 stabilization, and Bax upregulation by ODN."
BAP1 decreases the amount of SP1.
| 1
BAP1 decreases the amount of SP1. 1 / 1
| 1

reach
"The knockdown or catalytic mutant form of BAP1 abolished the ODN-induced upregulation of Sp1 and Bax expression."
BAP1 affects SNAI1
| 3
BAP1 increases the amount of SNAI1. 3 / 3
| 3

reach
"Loss of BAP1 has been reported to downregulate the expression of Snail, promoting ccRCC cells towards mesenchymal-epithelial transition 25."

reach
"BAP1 knockout in ccRCC cells also downregulated the expression of transcriptional repressor protein Snail and decreased the activity of Rho family GTPases, promoting the cells to undergo mesenchymal-epithelial transition."

reach
"BAP1 knockout in ccRCC cells also downregulated the expression of transcriptional repressor protein Snail and decreased the activity of Rho family GTPases, promoting the cells to undergo mesenchymal-epithelial transition."
BAP1 affects RAR
| 3
BAP1 inhibits RAR. 3 / 3
| 3

reach
"See also Figures equirement of BAP1 for ASXL1 mediated RAR repression."

reach
"(B) R ASXL1 for BAP1 mediated RAR repression."

reach
"BAP1 Is a Histone H2B Specific DUB In Vivo and In Vitro To gain insight into themechanisms of RAR repressionmediated by ASXL1 and BAP1, we first determined whether the deubiquiti- nase (DUB) activity [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
BAP1 affects PTGER4
| 3
BAP1 increases the amount of PTGER4. 3 / 3
| 3

reach
"In addition, BaP1 up-regulates EP4 receptor expression."

reach
"These findings are supported by the increased EP4 protein expression following BaP1 stimulation in our experimental model, confirming the ability of this receptor to activate FLSs."

reach
"BaP1 also up-regulates EP4 receptor expression."
BAP1 affects PSME3
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
BAP1 affects PBRM1, and SETD2
| 3
SETD2 binds PBRM1 and BAP1. 3 / 3
| 3

sparser
"The mutations in the chromatin regulators PBRM1 , BAP1 , and SETD2 were differentially associated with altered expression patterns of large numbers of genes when compared to samples bearing a background of VHL mutation ( xref , xref )."

sparser
"In fact this association with chromosome 3p loss has recently been strengthened by several publications demonstrating that 4 out of five ( VHL , PBRM1 , SETD2 and BAP1 ) of the most frequently mutated genes associated with CCRCC are on chromosome 3p xref – xref and that the mutation of either SETD2 , BAP1 or PBRM1 associates with poorer prognosis or progression in those patients xref , xref , xref ."

sparser
"On univariable analysis SETD2 (HR: 3.30), BAP1 (HR: 2.44) and PBRM1 (HR: 1.78) were significantly associated with a higher risk of metastases."
BAP1 affects OCM
| 3
| 2

sparser
"These findings suggest that BAP1 alterations may be associated with a more aggressive OM phenotype."

sparser
"Germline BAP1 mutations are associated with a more aggressive OM phenotype and a recurrent phenotypic complex of c utaneous/ o cular m elanoma, atypical m elanocytic proliferations and o ther internal n eoplasms (ie."
OCM binds BAP1 and CM. 1 / 1
| 1

sparser
"In conclusion, our evidence suggests that germline BAP1 inactivation is preferentially associated with metastatic OM and hereditary CM-OM in a small but significant proportion of cases."
BAP1 affects Nevus
| 3
BAP1 inhibits Nevus. 3 / 3
| 3

reach
"BINs are highly penetrant, present in up to 90% of mutation carriers, and typically present as multiple, skin colored or reddish-brown, dome-shaped melanocytic tumors (also or previously called BAP1-inactivlated melanocytic tumors, Wiesner nevi, BAPomas, nevoid melanoma-like melanocytic proliferations, BAP1 mutant Spitz nevi, and melanocytic BAP1-mutated atypical intradermal tumors)."

reach
"Morphology of this secondary clone strictly depends on the type of second genetic hit: inactivation of the BAP1 (BRCA1-associated protein) gene is the hallmark of BAP1-inactivated nevus (BIN) (33, 34); gain-of-function mutations of CTNNB1 or loss of APC is found in deep penetrating nevus (DPN) (35, 36); loss-of-function of PRKAR1A is typical of pigmented epithelioid melanocytoma (PEM) (37, 38)."

reach
"Pathologists can enable identification of patients at risk by recognizing BAP1-inactivated nevi."
BAP1 affects NUMB
| 3
| 3

sparser
"Third, similar to N1ICD, NUMB could bind to BAP1 N-terminal or C-terminal part ( xref )."

sparser
"First, both exogenously expressed and endogenous BAP1 could bind to endogenous NUMB ( xref )."

sparser
"We next examined whether BAP1 binds to NUMB and whether this association regulates N1ICD stability."
BAP1 affects KDM5C, PBRM1, and SETD2
| 3
| 3

sparser
"Our study indicates that mutations of PBRM1 , SETD2 , BAP1 and/or KDM5C in kidney cancers are associated with advanced stage, grade and tumor invasiveness."

sparser
"Mutations in PBRM1, SETD2, BAP1 , and KDM5C are associated with tumors of advanced stage (odds ratio range 2.34 to 6.40, 2.5, 2.62 to 4.57, 7.6, respectively)."

sparser
"We and others have reported the associations of PBRM1 , SETD2 , BAP1 , and KDM5C mutations with advanced stage, grade, and tumor invasiveness [ xref , xref ], and discovered that SETD2 and BAP1 mutations are associated with lower cancer-specific survival rates [ xref , xref ]."
BAP1 affects JUN
| 3
BAP1 activates JUN. 3 / 3
| 3

reach
"Furthermore, we also demonstrated that hyperactivity of ERK1/2 and JNK/c-Jun signaling induced by BAP1 downregulation was necessary for ICC cell proliferation, cell cycle progression, and invasion in vitro and in vivo using the inhibitors of the ERK1/2 pathway (U0126) and JNK/c-Jun pathway (SP600125)."

reach
"These data clearly suggested that hyperactivity of ERK1/2 and JNK/c-Jun signaling induced by BAP1 downregulation is necessary for ICC cell proliferation, cell cycle progression, and invasion."

reach
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways."
BAP1 affects IRF8
| 3
BAP1 activates IRF8. 3 / 3
| 3

reach
"We also examined whether ASXL1-MT and BAP1 upregulated posterior HOXA genes and IRF8 in human RUNX1-ETO cells by qRT-PCR."

reach
"ASXL1-MT and BAP1 induces upregulation of HOXA genes and IRF8."

reach
"In addition to posterior Hoxa genes, we found that coexpression of ASXL1-MT and BAP1 in c-Kit + cells led to upregulation of Irf8, a master regulator of monocyte differentiation, at both mRNA and protein levels 37."
BAP1 affects HAT1
2 | 1
2 | 1

No evidence text available

reach
"HAT1 and COPI interaction with BAP1 is observed in the cytoplasm and not in the nuclear fractions (Figs 1A and 1B and 4)."
| PMC

No evidence text available
BAP1 affects FGFBP1
| 3
BAP1 increases the amount of FGFBP1. 3 / 3
| 3

reach
"WT BAP1, but not mutant BAP1 C91S, obviously elevated the KLF5 and FGF-BP protein levels and inhibited the p27 protein levels in both cell lines (XREF_FIG)."

reach
"As expected, knockdown of BAP1, HCF-1, OGT or KLF5 markedly increased p27 and decreased FGF-BP protein levels in HCC1806 cells (XREF_FIG)."

reach
"In addition, expression of KLF5, CyclinD1, and FGF-BP1 was increased by BAP1 overexpression and decreased by BAP1 knockdown."
BAP1 affects EIF1AX, and SF3B1
| 3
| 3

sparser
"Uveal melanoma (UM) is a relative rare disease and has a high mortality rate due to metastasis in about half of all patients within 15 y after diagnosis. xref , xref , xref It is the most common primary intra‐ocular malignancy in adults in the Western world. xref UM specific mutations in the alpha subunit genes GNAQ and GNA11 are described as well as mutations in BAP1 , SF3B1 , and EIF1AX . xref , xref , xref Mutations in the latter 3 genes are found in ∼75% of all UM and are useful for prognostication of patients. xref , xref , xref BAP1 ‐mutated UM gives rise to early‐onset metastasis whereas SF3B1 ‐mutated UM gives rise to late‐onset metastasis and EIF1AX ‐mutated UM hardly metastasizes. xref Mutations in PLCB4 and CYSLTR2 are described in UM in a mutually exclusive manner to GNAQ or GNA11 mutations but so far have not been associated with prognosis. xref , xref Copy number alterations in chromosomes 1, 3, 6, and 8 are correlated with prognosis of the UM patient. xref , xref UM with EIF1AX , SF3B1 and BAP1 mutations are associated with unique chromosomal patterns, suggesting distinct UM subclasses."

sparser
"The Rotterdam Ocular Melanoma Study Group investigated the association of EIF1AX , SF3B1 and BAP1 mutation with disease-free survival and metastatic risk of patients with uveal melanoma. xref In their recent article, they published a Kaplan-Meier survival probability curve regarding these mutations."

sparser
"Other significantly mutated genes in UM are BAP1 , EIF1AX and SF3B1 , which also form a second mutually‐exclusive subgroup xref (Table  xref )."
BAP1 affects E2F1
| 1 2
| 1 2

sparser
"Furthermore, in chromatin immunoprecipitation experiments, BAP1 could bind to E2F1 responsive promoters and the localization of BAP1 to E2F1-responsive promoters is host cell factor-1 dependent."

reach
"BAP1 binds to E2F1-responsive gene promoters through HCF-1 and upregulates their expression, and BAP1 KO inhibits the growth of uveal melanoma cells ."

sparser
"Pan et al. have indicated that BAP1 may interact with promoters regulated by E2F1 and affect the cell cycle progression genes controlled by E2F. xref "
BAP1 affects E2F
| 3
| 3

sparser
"14 At present, it is not known whether BAP1 directly interacts with ubiquitinated E2F transcription factors."

sparser
"In addition, co-immunoprecipitation experiments have shown that BAP1 directly associates with E2F family members, suggesting a possible additional direct control of these transcriptional factors by BAP1 xref ."

sparser
"Presently, however, direct evidence of BAP1 interacting with ubiquitinated E2F transcription factors is still lacking."
BAP1 affects COPI complex
| 3
BAP1 binds COPI complex. 3 / 3
| 3

reach
"Overall, the loss of mass of COPA on blot could not be tied to the loss of ubiquitin and additional experiments are required to explain this observation.To exclude that the BAP1 interaction with the COPI complex is an artifact of overexpression of the exogenous BAP1 construct, we immunoprecipitated endogenous BAP1 using different antibodies against BAP1 in cell lysate from HeLa FRT parental cells (Fig 5D)."
| PMC

reach
"It was intriguing that BAP1 has such a sequence in its C-terminus, but our mutational analysis suggests that the interaction between BAP1 and the COPI complex is not mediated through the C-terminal KxKxx sequence in BAP1."
| PMC

reach
"AP-immunoblotting of GFP-COPE shows interaction of COPA as expected, however BAP1 cannot be observed which is probably due to the low levels of BAP1 that bind to the COPI complex (Fig 5E)."
| PMC
BAP1 affects CCR5
| 3
BAP1 increases the amount of CCR5.
| 2
Mutated BAP1 increases the amount of CCR5. 2 / 2
| 2

reach
"Additionally, the mechanism by which BAP1 mutation leads to increased expression of CCR5 and its ligands remains to be investigated."

reach
"We demonstrated that BAP1 mutation led to increased expression of CCR5 on Tregs and tumor cells."
BAP1 binds CCR5.
| 1
| 1

reach
"The association between BAP1 and CCR5 or its ligands was assessed by immunohistochemistry, flow cytometry, real-time PCR and ELISA."
BAP1 affects CAMP
| 3
| 3

sparser
"However, the estimated charge of the Bap1 protein monomer is −12.9 at pH 7.0 (), providing a possible explanation for the binding of the LL-37 peptide to the negatively charged target protein, the Bap1 protein, by an electrostatic interaction."

sparser
"We suggest that due to PmB treatment of the bacterial strain, the OmpT LDV residues become exposed during biogenesis on the surface of the OMVs, allowing for binding of the Bap1 protein and thereby promoting LL-37 binding to Bap1."

sparser
"The mechanism(s) of interaction between the LL-37 and the Bap1 protein is under investigation in our laboratory."
BAP1 affects BIRC5
| 1 2
| 1 2

reach
"Furthermore, co-immunoprecipitation experiments were done, but did not demonstrate direct protein protein interaction between BAP1 and survivin (data not shown)."

sparser
"Furthermore, co-immunoprecipitation experiments were done, but did not demonstrate direct protein-protein interaction between BAP1 and survivin (data not shown)."

sparser
"In untreated A375 and C918 cells, the loss of BAP1 was associated with a dramatic reduction in survivin levels ( xref , control “C” lanes), consistent with the prior analysis."
BAP1 affects BARD1, and E3_Ub_ligase
| 3

sparser
"They found that BAP1 did not appear to be involved in the deubiquitination of the BRCA1/BARD1 complex,29 and BAP1-mediated growth suppression in vitro was shown to be independent of wild-type BRCA1.4 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"According to this hypothesis, Nishikawa et al showed that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of the BRCA1/BARD1 complex, and that BAP1 and BRCA1/BARD1 mutually regulate ubiquitination in the cell cycle and in the DNA damage response pathway. xref According to the present state of knowledge, it is reasonable to speculate that BAP1 may exert a regulatory role in the DNA damage response."

sparser
"Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1/BARD1."
BAP1 affects ATF3
| 1 1 1
BAP1 inhibits ATF3.
| 1 1
BAP1 inhibits ATF3. 2 / 2
| 1 1

reach
"In RCC cells, BAP1 inhibited glucose deprivation-induced apoptosis through interaction with the activating transcription factor 3 (ATF3) and DNA damage-inducible transcript 3 protein (DDIT3) gene promoters in a polycomb complex protein BMI-1-dependent manner ."

eidos
"BAP1 repressed glucose deprivation-induced ER stress cell death by inhibiting transcription of two major UPR-TFs , activating transcription factor 3 ( ATF3 ) and C / EBP homologous protein ( CHOP ) ( 70 ) ."
BAP1 binds ATF3.
| 1
DDIT3 binds ATF3 and BAP1. 1 / 1
| 1

sparser
"Mechanically, we show that BAP1 represses metabolic stress-induced UPR and cell death through activating transcription factor 3 (ATF3) and C/EBP homologous protein (CHOP), and reveal that BAP1 binds to ATF3 and CHOP promoters and inhibits their transcription."
BAP1 affects ASXL proteins
| 3
BAP1 binds ASXL proteins. 3 / 3
| 3

reach
"The ASXL proteins directly interact with BAP1, KDM1A, NHRs and NCOA1 to target specific genomic loci using histone modifications."

reach
"The interaction between BAP1 and the ASXL proteins is mediated by the ~ 120-residue DEUBAD (DEUBiquitinase ADaptor) domain of the ASXL proteins [XREF_BIBR], and this region is sufficient for activation of BAP1 in in vitro H2AK119ub1 nucleosome deubiquitination assays [XREF_BIBR]."

reach
"ASXL proteins interact with BAP1 to form the polycomb repressive deubiquitinase (PR-DUB) complex, which removes monoubiquitin from histone 2A at lysine 119 (H2AK119) [XREF_BIBR, XREF_BIBR]."
BAP1 affects ADGRB1
| 1 2
| 1 2

reach
"Interaction between BAI1 and BAP1 was mediated by a QTEV motif in the carboxy-terminal region of BAI1 and PDZ domains of BAP1."

sparser
"Using a two-hybrid system, we isolated a cDNA that encodes a protein, named BAP1 (BAI1-associated protein), which interacts with the cytoplasmic region of BAI1."

sparser
"Interaction between BAI1 and BAP1 was mediated by a QTEV motif in the carboxy-terminal region of BAI1 and PDZ domains of BAP1."
ASXL2 affects ASXL1
| 3
ASXL1 binds ASXL2 and BAP1. 3 / 3
| 3

sparser
"Although both ASXL1 and ASXL2 have been identified to bind to BAP1, recent biochemical characterization of BAP1 complexes by xref suggest that BAP1 forms two mutually exclusive complexes with ASXL1 and ASXL2."

sparser
"Depletion of the PHD fingers did not affect the stability of ASXL1 or ASXL2, or the interaction between ASXL1, ASXL2, and BAP1 (Additional file xref : Fig. S2A, B)."

sparser
"Proteomic analysis of BAP1 interacting partners using this system revealed that BAP1 interacts with ASXL1 and ASXL2 in vivo as well as host cell factor 1 (HCF-1), O-GlcNAc transferase (OGT), and Forkhead box protein K1/2 (FOXK1/2) ( xref )."
ADGRB1 affects BAP1
| 1 2
| 1 2

reach
"Interaction between BAI1 and BAP1 was mediated by a QTEV motif in the carboxy-terminal region of BAI1 and PDZ domains of BAP1."

sparser
"Using a two-hybrid system, we isolated a cDNA that encodes a protein, named BAP1 (BAI1-associated protein), which interacts with the cytoplasmic region of BAI1."

sparser
"Interaction between BAI1 and BAP1 was mediated by a QTEV motif in the carboxy-terminal region of BAI1 and PDZ domains of BAP1."
2 |
Hsa-miR-200c-3p decreases the amount of BAP1. 2 / 2
2 |

No evidence text available

No evidence text available
2 |
Hsa-miR-200b-3p decreases the amount of BAP1. 2 / 2
2 |

No evidence text available

No evidence text available
2 |
Hsa-miR-200a-3p decreases the amount of BAP1. 2 / 2
2 |

No evidence text available

No evidence text available
2 |
Hsa-miR-141-3p decreases the amount of BAP1. 2 / 2
2 |

No evidence text available

No evidence text available
Class 2 affects GEP
| 2
BAP1 binds GEP and class 2. 2 / 2
| 2

sparser
"12,36,39 BAP1 mutation is associated with monosomy 3 or class 2 GEP tumors and impart poor survival."

sparser
"BAP1 is associated with monosomy 3, poor prognosis, and class 2 GEP tumors, while EIF1AX is associated with class 1 GEP tumors and good prognosis."
Cas9 affects BAP1
| 2
Cas9 activates BAP1. 2 / 2
| 2

reach
"32Dcl3 cells were transduced with Cas9 and two independent guide RNAs (gRNAs) targeting Bap1."

reach
"Next, we profiled PANC1 cells that were exposed to IR in the context of BAP1 downregulation (sgBAP1) mediated by CRISPR/Cas9."
Bisphenol A affects BAP1
2 |
Bisphenol A increases the amount of BAP1. 2 / 2
2 |

No evidence text available

No evidence text available
| 2

reach
"This result also further supports the assertion that the that the driving interaction for the binding of betulinic acid to the metalloproteinase BaP1 is the contact between the Zn2+ ion and the carboxylate group."

reach
"To determine the contributions of enthalpy and entropy to the binding of BaP1 and betulinic acid [49,50], we performed equilibrium simulations in the free energy minima A and B, as well as the unbound state."
Ab affects YUH1
| 2
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
VHL affects KDM5C
| 2
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
VEGFA affects BAP1
| 2
| 2

reach
"This study investigated the prognostic role of BAP1 in matched cytology and surgical specimens and aimed to investigate the association between BAP1 and the established prognostic marker VEGFA from a cohort of 81 patients."

reach
"Levels of VEGFA, an established prognostic factor in MM, were correlated with survival to assess potential interaction of BAP1 and VEGFA."
ULD affects YUH1
| 2
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
UCHL5 affects BAP1
| 1 1
| 1 1

sparser
"These differences can rationalize why GK13S does not bind to UCHL5 and BAP1."

reach
"This suggests that these residues play functionally important roles that have yet to be defined but may include binding of specific substrates or regions of partner proteins within their respective UCH37 and BAP1 complexes."
UBC affects BAP1
2 |
2 |

No evidence text available

No evidence text available
TP53 affects SETD2
| 2
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
TBCB affects BAP1
| 2
| 2

sparser
"Here we show that BAP1 assembles multiprotein complexes containing numerous transcription factors and cofactors, including HCF-1 and the transcription factor Yin Yang 1 (YY1)."

sparser
"BAP1 is associated with transcriptionally active chromatin and interacts with several transcription factors and cofactors, thus acting as a chromatin scaffold for chromatin-remodeling complexes ( xref , xref )."
SMAD6 affects BAP1
| 2
SMAD6 activates BAP1. 2 / 2
| 2

reach
"Recent biochemical and cell based studies have showed that UBE2O ubiquitinates SMAD6 during bone morphogenetic protein signaling, induces cytoplasmic sequestration of nuclear BAP1, and coordinates endosomal protein trafficking."

reach
"Recent biochemical and cell based studies have shown that UBE2O ubiquitinates SMAD6 during bone morphogenetic protein signaling (Zhang et al., 2013a), induces cytoplasmic sequestration of nuclear BAP1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
SLC7A11 affects BAP1
| 2
SLC7A11 increases the amount of BAP1. 2 / 2
| 2

reach
"p53 binds to the SLC7A11 promoter, directly suppressing its transcription; the nuclear deubiquitinase (DUB) BAP1 represses SLC7A11 transcription by removing histone 2A ubiquitination from the SLC7A11 promoter; and KEAP1 represses SLC7A11 transcription through degrading NRF2, a master transcription factor of antioxidant response and regulator of SLC7A11."

reach
"A luciferase assay with the SLC7A11 promoter showed that overexpression of BAP1 WT, but not its C91A mutant, decreased luciferase activity, whereas BAP1 knockdown increased it, indicating that BAP1 regulates SLC7A11 transcription (XREF_SUPPLEMENTARY - XREF_SUPPLEMENTARY)."
SH3PXD2A affects BAP1
| 2
SH3PXD2A decreases the amount of BAP1. 2 / 2
| 2

reach
"Loss of BAP1 expression by ICC and homozygous deletion of p16/CDKN2a by FISH are specific but not sensitive for MM."

reach
"Loss of BAP1 expression by ICC and homozygous deletion of p16/CDKN2a by FISH are specific but not sensitive for MM."
SETD2 affects TP53
| 2
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
SETD2 affects MTOR
| 2
SETD2 binds MTOR and BAP1. 2 / 2
| 2

sparser
"By comparing tumours which cluster with the cell lines based on CNAs with tumours that cluster away from the cell lines, we found that the tumours likely to be best represented by the cell lines carry hallmarks of aggressive disease, such as higher stage, higher grade, greater extent of CNAs, and more frequent mutations in genes such as SETD2, BAP1 and MTOR , which have been associated with more aggressive disease and poorer outcomes."

sparser
"These tumours also display a higher extent of copy number aberrations (mean fraction genome altered: 19% versus 12%), and more frequent mutations in genes such as BAP1 (12.3% versus 5.4%), SETD2 (12.7% versus 8.1%) and MTOR (6% versus 4.7%), which are associated with more aggressive disease (though only BAP1 has a statistically significant difference— P value 0.025, Fisher's exact test)."
RITA1 affects BAP1
| 2
RITA1 inhibits BAP1. 2 / 2
| 2

reach
"[RITA selectively inhibits proliferation of BAP1-deficient cutaneous melanoma cells in vitro]."

reach
"The p53 activator RITA from the compound library was shown to selectively inhibit the viability of BAP1 knockout cells."
RING affects BRCA1
| 2
BRCA1 binds BAP1 and RING. 2 / 2
| 2

sparser
"BAP1 interacts with the RING domain of human BRCA1, a predominately nuclear, ovarian and breast cancer susceptibility gene product with tumor suppressor activity."

sparser
"The BRCA1 RING domain also binds BAP1 (BRCA1-associated protein-1), a de-ubiquitinating enzyme 26 ."
RAG2 affects BAP1
| 2
RAG2 inhibits BAP1. 2 / 2
| 2

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14, which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7."

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14 which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7 [XREF_BIBR]."
RAD21 affects BAP1
| 2
| 2

sparser
"Nevertheless, only mutated RAD21 and BAP1 were associated with PD-L1 expression in tumor cells."

sparser
"The somatically mutated RAD21 and BAP1 are associated with PD-L1 expression in ccRCC tumor cells and might serve as a potential predictor of the response to immunotherapy with PD-1/PD-L1 inhibitors in ccRCC patients."
RA-9 affects BAP1
| 2
RA-9 inhibits BAP1. 2 / 2
| 2

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14 which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7 [XREF_BIBR]."

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14, which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7."
PTEN affects ASXL1
| 2
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
PRC1 affects BAP1
| 2
| 2

sparser
"Recent CRISPR screening studies by Dixit’s group further identified that depletion of PRC1 subunit Ring1B (but not Ring1A) could rescue the cell death induced by loss of BAP1 xref , indicating that there is a robust and direct epigenetic dynamic occurring between the PRC1 and BAP1 complexes that require a balanced state to properly regulate gene expression and determine cell fate."

sparser
"Given that the composition and expression of PRC1 and BAP1 complexes changes extensively during development ( xref ; xref , xref ; xref ; xref ), in future work, it will be interesting to investigate how the balance between these two opposing activities is regulated at different developmental stages and how cell type-specific H2AK119ub1 levels influence the transcriptional potential of the genome."
PGR affects ASXL1
| 2
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
PBRM1 affects H3K36me3
| 2
PBRM1 binds BAP1 and H3K36me3. 2 / 2
| 2

sparser
"We previously evaluated the prognostic impact of BAP1 and PBRM1 to define epigenetic subtypes of clear cell renal cell carcinoma.( xref , xref ) To examine the association of H3K36me3 with BAP1 and PBRM1 protein expression, we evaluated the expression of all 3 markers dichotomized as positive versus negative in clear cell renal cell carcinoma samples with available staining ( xref )."

sparser
"Association of H3K36me3 with Epigenetic Tumor Suppressors BAP1 and PBRM1."
PARP1 affects BAP1
| 1
| 1

reach
"Poly(ADP-ribose) polymerase 1 (PARP1) interacts with and recruits BAP1 to damage sites, with BAP1 recruitment peaking after the DDB2 and XPC damage sensors."
OGT affects HCFC1
| 2
HCFC1 binds OGT and BAP1. 2 / 2
| 2

reach
"The fact that HCF-1 acts as an important modulator of cellular circadian rhythm [XREF_BIBR] raises the possibility that the OGT, HCF-1, and BAP1 complex interacts with the BMAL1, CLOCK, and PER complex to modulate circadian rhythm."

reach
"Within the BAP1/HCF-1/OGT complex, HCF1 is modified by O-GlcNacylation, the addition of single O-linked N-acetylglucosamine moiety to specific serine or threonine residues (50,51)."
NP_828865 affects BAP1
2 |
BAP1 binds NP_828865. 2 / 2
2 |

No evidence text available

No evidence text available
NEAT-1 affects BAP1
| 2
NEAT-1 activates BAP1. 2 / 2
| 2

reach
"Using qPCR and primer sets that target both NEAT1_1 and NEAT1_2 (Total NEAT1), or NEAT1_1 alone, we observed an increase in both total NEAT-1 and NEAT1_1 with siRNA mediated modulation of BAP1 (Additional file 3)."

reach
"The mechanisms by which NEAT-1 can modulate BAP1 effects are unknown."
MTOR affects SETD2
| 2
SETD2 binds MTOR and BAP1. 2 / 2
| 2

sparser
"By comparing tumours which cluster with the cell lines based on CNAs with tumours that cluster away from the cell lines, we found that the tumours likely to be best represented by the cell lines carry hallmarks of aggressive disease, such as higher stage, higher grade, greater extent of CNAs, and more frequent mutations in genes such as SETD2, BAP1 and MTOR , which have been associated with more aggressive disease and poorer outcomes."

sparser
"These tumours also display a higher extent of copy number aberrations (mean fraction genome altered: 19% versus 12%), and more frequent mutations in genes such as BAP1 (12.3% versus 5.4%), SETD2 (12.7% versus 8.1%) and MTOR (6% versus 4.7%), which are associated with more aggressive disease (though only BAP1 has a statistically significant difference— P value 0.025, Fisher's exact test)."
L3MBTL2 affects BAP1
| 1 1
| 1 1

sparser
"By targeting atypical polycomb protein L3MBTL2, BAP1 interacts with and stabilizes L3MBTL2, co-occupying and maintaining H4K20me1 at target gene loci, such as EZH2 locus."

reach
"By targeting atypical polycomb protein L3MBTL2, BAP1 interacts with and stabilizes L3MBTL2, co-occupying and maintaining H4K20me1 at target gene loci, such as EZH2 locus."
KMT2C affects KDM6A
| 2
KDM6A binds KMT2C and BAP1. 2 / 2
| 2

sparser
"We found that cancer hotspot mutations in the MLL3 amino terminal PHD repeats disrupt its association with the BAP1 complex and result in reduced MLL3 and UTX recruitment to enhancers misregulating the epigenetic chromatin state at these loci."

sparser
"Mutations within the MLL3 PHD domains abolish the interaction between the BAP1 tumor suppressor complex and MLL3-UTX, which leads to attenuation of enhancer activity."
KDM5C affects VHL
| 2
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
ITPR1 affects BAP1
| 2
| 2

sparser
"Taken together, these results indicated that DIM could induce ferroptosis to exert anti-cancer effects via BAP1-IP3R axis, suggesting its effective therapeutic potential in gastric cancer."

sparser
"3,3'-Diindolylmethane induces ferroptosis by BAP1-IP3R axis in BGC-823 gastric cancer cells."
Histone affects H2AC20
| 2
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."
HDAC_I affects BAP1
| 2
HDAC_I inhibits BAP1. 2 / 2
| 2

reach
"Histone deacetylase (HDAC) inhibitors, such as the readily available valproic acid, can reverse this effect of BAP1 loss and may play a role in treated patients with high-risk class 2 uveal melanomas in an adjuvant setting prior to the emergence of overt metastatic disease [XREF_BIBR]."

reach
"Histone deacetylase (HDAC) inhibitors have been shown to reverse the phenotypic effects of BAP1 loss by inducing morphologic differentiation and transition from a high-risk to a low-risk gene expression profile in uveal melanoma cells."
HCFC1 affects OGT
| 2
HCFC1 binds OGT and BAP1. 2 / 2
| 2

reach
"The fact that HCF-1 acts as an important modulator of cellular circadian rhythm [XREF_BIBR] raises the possibility that the OGT, HCF-1, and BAP1 complex interacts with the BMAL1, CLOCK, and PER complex to modulate circadian rhythm."

reach
"Within the BAP1/HCF-1/OGT complex, HCF1 is modified by O-GlcNacylation, the addition of single O-linked N-acetylglucosamine moiety to specific serine or threonine residues (50,51)."
H3K36me3 affects PBRM1
| 2
PBRM1 binds BAP1 and H3K36me3. 2 / 2
| 2

sparser
"We previously evaluated the prognostic impact of BAP1 and PBRM1 to define epigenetic subtypes of clear cell renal cell carcinoma.( xref , xref ) To examine the association of H3K36me3 with BAP1 and PBRM1 protein expression, we evaluated the expression of all 3 markers dichotomized as positive versus negative in clear cell renal cell carcinoma samples with available staining ( xref )."

sparser
"Association of H3K36me3 with Epigenetic Tumor Suppressors BAP1 and PBRM1."
H2AC20 affects Histone
| 2
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."
GEP affects class 2
| 2
BAP1 binds GEP and class 2. 2 / 2
| 2

sparser
"12,36,39 BAP1 mutation is associated with monosomy 3 or class 2 GEP tumors and impart poor survival."

sparser
"BAP1 is associated with monosomy 3, poor prognosis, and class 2 GEP tumors, while EIF1AX is associated with class 1 GEP tumors and good prognosis."
Flag affects BAP1
| 1 1
| 1 1

sparser
"Moreover, we observed that Flag-ASXL1 interacted with endogenous HCF-1, FOXK1 and FOXK2 in BAP1 knockdown HEK293T cells (Fig.  xref G), and that Flag-BAP1 interacted with endogenous HCF-1, FOXK1, and FOXK2 in ASXL1 knockdown HEK293T cells (Fig.  xref H)."

reach
"Full-length BAP1 (1-729), the BAP1 N terminus (1-240) and the BAP1 C terminus (597-729) interacted with KLF5-3 x Flag (XREF_FIG)."
Dbi affects BAP1
| 2
Dbi phosphorylates BAP1. 2 / 2
| 2

sparser
"Interestingly, ODN-induced BAP1 phosphorylation and DNA damage were modulated by the production of mitochondrial reactive oxygen species (ROS)."

sparser
"Mechanistically, ODN induced BAP1 phosphorylation and enhanced Sp1-BAP1 interaction, resulting in Sp1 ubiquitination and degradation."
DIDO1 affects BAP1
| 1 1
| 1 1

sparser
"BAP1 binds to DIDO1 and stabilizes DIDO1 through de-ubiquitination."

reach
"BAP1 binds to DIDO1 and stabilizes DIDO1 through de-ubiquitination."
CRISPR affects BAP1
| 2
CRISPR inhibits BAP1. 2 / 2
| 2

reach
"We also identified an association of clinical BAP1 mutations with high B7H4/3 expression, and validated it as a causal relationship between CRISPR-mediated BAP1 loss and B7H4 upregulation in iCCA cell line models."

reach
"Artegiani et al. found that after loss-of-function of BAP1 by CRISPR/Cas9 in normal human cholangiocyte organoids, the cells became more motile and fused with other organoids, features that resemble the metastatic invasion of cancer."
| PMC
CDKN2A affects BAP1
| 2
CDKN2A inhibits BAP1. 2 / 2
| 2

reach
"The 2 peritoneal mesothelioma cases demonstrated loss of BAP1 but not p16."

reach
"To that end, a systematic literature review was performed of articles dealing with a loss of BRCA1-associated protein 1 (BAP1), methylthioadenosine (MTAP), 5-hydroxymethylcitosine (5-hmC), glucose transporter 1 (GLUT1), insulin like-growth factor II messenger RNA-binding protein 3 (IMP3), enhanced zeste homologue 2 (EZH2) staining, cyclin-dependent kinase inhibitor 2A (CDKN2A) homozygous deletion (HD) testing, soluble mesothelin, and microRNA quantification in cytological samples for the diagnosis of MPM versus reactive atypical mesothelial cells."
CD3EAP affects BAP1
| 1 1
| 1 1

sparser
"BAP1 interacts with CAST in UM cells, and CAST and its subsequent calpain pathway may mediate BAP1-related cell migration regulation."

reach
"BAP1 interacts with CAST in UM cells, and CAST and its subsequent calpain pathway may mediate BAP1 related cell migration regulation."
CCDC6 affects BAP1
| 2
| 2

sparser
"Coiled-coil domain containing 6 (CCDC6) interacts with BAP1 and has been reported to regulate both homologous recombination and PARP inhibitor sensitivity."

sparser
"The interaction between CCDC6 and BAP1 was confirmed in MPM cells, by co-immunoprecipitation."
BRCA1 affects RING
| 2
BRCA1 binds BAP1 and RING. 2 / 2
| 2

sparser
"BAP1 interacts with the RING domain of human BRCA1, a predominately nuclear, ovarian and breast cancer susceptibility gene product with tumor suppressor activity."

sparser
"The BRCA1 RING domain also binds BAP1 (BRCA1-associated protein-1), a de-ubiquitinating enzyme 26 ."
BRCA affects BAP1
| 2
| 2

reach
"We assessed whether tumour BRCA associated protein 1 (BAP1) status is a predictive biomarker for survival in patients receiving first-line combination platinum/pemetrexed therapy.introduction METHODS: PM cases (n=114) from Aalborg, Denmark were stained for BAP1 on tissue microarrays."

reach
"Recent work has shown that BRCA-associated protein 1 (BAP1), which functions as part of the polycomb repressive deubiquitinase complex that removes monoubiquitination on H2AK119, regulates SLC7A11, a subunit of the heterodimeric system x cystine-glutamate antiporter."
BRAP affects BAP1
| 1
BRAP inhibits BAP1. 1 / 2
| 1

reach
"Morphology of this secondary clone strictly depends on the type of second genetic hit: inactivation of the BAP1 (BRCA1-associated protein) gene is the hallmark of BAP1-inactivated nevus (BIN) (33, 34); gain-of-function mutations of CTNNB1 or loss of APC is found in deep penetrating nevus (DPN) (35, 36); loss-of-function of PRKAR1A is typical of pigmented epithelioid melanocytoma (PEM) (37, 38)."
BCR affects BAP1
| 2
| 2

reach
"Likewise, the mutants carrying box-1 (residues 166-208) of the tau-isoform, a rat counterpart of mouse Bcr binding protein Bap-1 [11], bound Bcr, while the mutants lacking box-1 did not.To further eva[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As shown in Figure 5, tyrosine phosphorylation of Bcr by Fes greatly reduced Bcr binding to Bap-1."
BAP1 affects vorinostat
| 2
| 2

reach
"BAP1 downregulation increases the sensitivity to HDACi in mesothelioma cell lines, and therefore, it would be important to see whether BAP1 aberrations modulate response to vorinostat in VANTAGE 014 study."

reach
"The BAP1 deficient developmental phenotype could be rescued using SAHA or specific depletion of HDAC4."
BAP1 affects viability migration ECA109 cells
| 2
BAP1 activates viability migration ECA109 cells. 2 / 2
| 2

eidos
"BAP1 promotes viability and migration of ECA109 cells through KLF5 / CyclinD1 / FGF-BP1 ."

eidos
"Erratum to : BAP1 promotes viability and migration of ECA109 cells through KLF5 / CyclinD1 / FGF-BP1 ."
BAP1 affects ubiquitination
| 1
BAP1 inhibits ubiquitination. 1 / 2
| 1

sparser
"NPM/B23 is stabilized by ubiquitination ( Sato et al., 2004 ), which is inhibited by BAP1 ( Nishikawa et al., 2009 )."
BAP1 affects olaparib
| 2
Mutated BAP1 activates olaparib. 2 / 2
| 2

reach
"A BAP1 mutant by alternative splicing resulting in a 54-bp deletion increased sensitivity to the PARP inhibitor olaparib."

reach
"Mutant BAP1 in combination with CCDC6 silencing enhanced the HR-DNA repair defects and sensitivity to olaparib."
BAP1 affects mitochondrial protein
| 2
BAP1 increases the amount of mitochondrial protein. 2 / 2
| 2

reach
"In pancreas, Bap1 knockout elevated Reg family pancreatitis markers (Graf et al., 2002) and significantly reduced mitochondrial protein expression (p = 2.3e-4)."

reach
"In pancreas, Bap1 knockout elevated Reg family pancreatitis markers, and significantly reduced mitochondrial protein expression (p = 2.3e-4) (XREF_FIG)."
BAP1 affects mastoparan-M
| 2
| 2

sparser
"The EZH2 inhibitor tazemetostat was administered to patients with BAP1 inactivated MPM (loss of nuclear expression)."

sparser
"Based on this model, EZH2 inhibition was tested in BAP1 inactivated MPMs [ xref ]."

reach
"It is concluded that MT-III and BaP1 induce a local inflammatory response associated with the synthesis of IL-1beta, IL-6 and MMPs."

reach
"These results indicated that BAP1 affects the expression of chemokines and cytokines, T and B cell migration, and activated inflammation associating with innate immunity."
BAP1 affects hydrolase
| 2
| 2

reach
"The BAP1 gene produces a 729 AA protein containing several functional domains: a ubiquitin C-terminal hydrolase (UCH) domain, a BRCA1-associated RING domain protein 1 (BARD1) binding domain, an HCF-C1 binding motif (HBM) and a nuclear localization signal ."

reach
"BAP1 enhances BRCA1 mediated inhibition of breast cancer cell growth and is the first nuclear localized ubiquitin carboxy-terminal hydrolase to be identified."

reach
"Modulation of Schwann cell homeostasis by the BAP1 deubiquitinase."

reach
"Overall, our results indicate Bap1 is required to restrict the spread of polycomb-associated histone modifications in Schwann cells and to promote myelin homeostasis in peripheral nerve."
BAP1 affects growth
| 1
BAP1 inhibits growth. 1 / 2
| 1

sparser
"It has been demonstrated that BAP1 inhibits tumor growth in vitro and in animal models [6,22] ."
BAP1 affects glucose
| 1 1
BAP1 inhibits glucose. 2 / 2
| 1 1

reach
"BAP1 inhibits glucose deprivation induced reactive oxygen species and ATP depletion, two cellular events contributing to the ER stress induced cell death."

sparser
"In RCC cells, BAP1 inhibited glucose deprivation-induced apoptosis through interaction with the activating transcription factor 3 ( ATF3 ) and DNA damage-inducible transcript 3 protein ( DDIT3 ) gene promoters in a polycomb complex protein BMI-1-dependent manner xref ."
| 2

reach
"Through a biochemical screen, we identified BAP1 catalytic inhibitors that inhibit truncated-ASXL1-driven leukemic gene expression and impair tumor progression in vivo."

reach
"Furthermore, our findings that BaP1 induced gene expression of COX-2 and mPGES-1 demonstrate that BaP1 can trigger transcriptional mechanisms in FLSs that result in increased protein expression."
| 1 1

sparser
"Investigating the cytoplasmic function of BAP1, the authors focused on the possible interaction between BAP1 and ER channels that control Ca 2+ flux to the cytoplasm."
| 1
| 1

reach
"BAP1 +/- fibroblasts indeed displayed a reduced level of Ca 2+ release from the ER, which was restored by re-expression of chimeric BAP1 mutants selectively targeting ER and cytoplasm, but not the nucleus."
| 2

reach
"The recent finding that BAP1 loss leads to defective differentiation and an arrested primitive phenotype in vertebrate development and uveal melanoma suggests that BAP1 loss can promote tumor progression by inducing cell dedifferentiation and stem like behavior."

reach
"Loss of BAP1 induces dedifferentiation of melanoma cells and the development of stem cell like characteristics XREF_BIBR, XREF_BIBR."
BAP1 affects class 2
| 2
BAP1 binds GEP and class 2. 2 / 2
| 2

sparser
"12,36,39 BAP1 mutation is associated with monosomy 3 or class 2 GEP tumors and impart poor survival."

sparser
"BAP1 is associated with monosomy 3, poor prognosis, and class 2 GEP tumors, while EIF1AX is associated with class 1 GEP tumors and good prognosis."

reach
"Recent studies show that BAP1 loss in cancer cells causes pivotal changes in cellular metabolism (16–21)."

reach
"Glycolytic alterations:. In addition to regulating nutrient homeostasis in normal cells and tissues, BAP1 loss increases cancer susceptibility by regulating cellular metabolism (55,62)."
BAP1 affects cell
| 2
BAP1 inhibits cell. 2 / 2
| 2

eidos
"These results indicated that BAP1 suppressed ICC cell proliferation , cell cycle progression , and invasion via inhibiting ERK1 / 2 and JNK / c-Jun signaling pathways ."

eidos
"Similarly , downregulation of BAP1 markedly increased cells in the S phase and reduced cells in the G0 / G1 phase , consistent with the results of a recent study that depletion of BAP1 resulted in a modest accumulation of host cell factor 1 , which promoted the transition from G1 to S phase41 ."
| 2

reach
"This result also further supports the assertion that the that the driving interaction for the binding of betulinic acid to the metalloproteinase BaP1 is the contact between the Zn2+ ion and the carboxylate group."

reach
"To determine the contributions of enthalpy and entropy to the binding of BaP1 and betulinic acid [49,50], we performed equilibrium simulations in the free energy minima A and B, as well as the unbound state."
BAP1 affects autophagy
| 1 1
| 1 1

eidos
"BAP1 antagonizes WWP1-mediated transcription factor KLF5 ubiquitination and inhibits autophagy to promote melanoma progression ."

reach
"BAP1 antagonizes WWP1 mediated transcription factor KLF5 ubiquitination and inhibits autophagy to promote melanoma progression."
BAP1 affects assembly constitutive BRCA1 foci
| 2
BAP1 activates assembly constitutive BRCA1 foci. 2 / 2
| 2

eidos
"We further found that BAP1 depletion suppressed the assembly of constitutive BRCA1 foci , which are associated with homologous recombination ( HR ) , but had minimal effect on gamma-H2AX foci and did not affect proteins associated with nonhomologous end joining , suggesting that BAP1 affects radiosensitivity in HNSCC by modifying HR ."

eidos
"In our study , we found that BAP1 depletion decreased the assembly of constitutive BRCA1 foci , which are associated with HR , but had minimal effect on gamma-H2AX foci ."
BAP1 affects activity
| 2
BAP1 inhibits activity. 2 / 2
| 2

sparser
"BAP1 interacts with BARD1 and BRCA1 and inhibits the E3 ligase activity of BRCA1/BARD1."

sparser
"The BRCA1 associated protein 1 (BAP1), a de-ubiquitinating enzyme (DUB), also inhibits the E3 ligase activity of BRCA1-BARD1 ( Nishikawa et al., 2009 )."
BAP1 affects VEGFA
| 2
| 2

reach
"This study investigated the prognostic role of BAP1 in matched cytology and surgical specimens and aimed to investigate the association between BAP1 and the established prognostic marker VEGFA from a cohort of 81 patients."

reach
"Levels of VEGFA, an established prognostic factor in MM, were correlated with survival to assess potential interaction of BAP1 and VEGFA."
BAP1 affects UCHL5
| 1 1
| 1 1

sparser
"These differences can rationalize why GK13S does not bind to UCHL5 and BAP1."

reach
"This suggests that these residues play functionally important roles that have yet to be defined but may include binding of specific substrates or regions of partner proteins within their respective UCH37 and BAP1 complexes."
BAP1 affects UBC
2 |
2 |

No evidence text available

No evidence text available
BAP1 affects TRAIL receptors
| 2
BAP1 decreases the amount of TRAIL receptors. 2 / 2
| 2

reach
"As a potential underlying mechanism, here we report that BAP1 negatively regulates the expression of TRAIL receptors: death receptors 4 (DR4) and 5 (DR5)."

reach
"These results suggest that BAP1 and YY1 cooperatively repress transcription of TRAIL receptors."
BAP1 affects TBCB
| 2
| 2

sparser
"Here we show that BAP1 assembles multiprotein complexes containing numerous transcription factors and cofactors, including HCF-1 and the transcription factor Yin Yang 1 (YY1)."

sparser
"BAP1 is associated with transcriptionally active chromatin and interacts with several transcription factors and cofactors, thus acting as a chromatin scaffold for chromatin-remodeling complexes ( xref , xref )."
BAP1 affects SETD2, and TP53
| 2
TP53 binds SETD2 and BAP1. 2 / 2
| 2

sparser
"Mutations in BAP1 , SETD2, and TP53 have been associated with inferior clinical results in those patients who undergo primary treatment with surgical resection [ xref ]."

sparser
"Among the prevalent non- VHL alterations, BAP1, SETD2 , and TP53 mutations are associated with a poor prognosis [ xref , xref , xref ]."
BAP1 affects RING
| 2
BRCA1 binds BAP1 and RING. 2 / 2
| 2

sparser
"BAP1 interacts with the RING domain of human BRCA1, a predominately nuclear, ovarian and breast cancer susceptibility gene product with tumor suppressor activity."

sparser
"The BRCA1 RING domain also binds BAP1 (BRCA1-associated protein-1), a de-ubiquitinating enzyme 26 ."
BAP1 affects RB1
| 2
BAP1 inhibits RB1. 2 / 2
| 2

reach
"A recent study demonstrated that, in contrast to wild-type animals, CDKN2A loss is not required for tumorigenesis in BAP1 +/- mice, They further demonstrated that BAP1 mediates Rb1 expression loss via epigenetic down-regulation independent of CDKN2A status [XREF_BIBR]."

reach
"MM cells from Bap1 +/- mice show biallelic inactivation of Bap1 and Cdkn2a independent downregulation of Rb."
BAP1 affects RAD51
| 2
BAP1 activates RAD51. 2 / 2
| 2

reach
"BAP1 depletion abrogates the binding of INO80 to replication forks and increases the formation of RAD51 foci following HU treatment."

reach
"BAP1 can deubiquitylate Ub-H2AK119 at the site of UV-induced DNA damage, and promote DNA repair by regulating the accumulation of repair proteins (BRCA1, RAD51, and RPA) (7)."
BAP1 affects RAD21
| 2
| 2

sparser
"Nevertheless, only mutated RAD21 and BAP1 were associated with PD-L1 expression in tumor cells."

sparser
"The somatically mutated RAD21 and BAP1 are associated with PD-L1 expression in ccRCC tumor cells and might serve as a potential predictor of the response to immunotherapy with PD-1/PD-L1 inhibitors in ccRCC patients."
BAP1 affects Polycomb
| 1 1
BAP1 inhibits Polycomb. 2 / 2
| 1 1

eidos
"BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation ."

reach
"BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation."
BAP1 affects Pancreatitis
| 2
| 2

reach
"Deletion or heterozygous loss of Bap1 in murine pancreata causes genomic instability, tissue damage, and pancreatitis with full penetrance."

reach
"Although LOH of additional genes within Chr 3p21.1 may contribute to tumor suppression, and loss of BAP1 in pancreatic cancer may be secondary—rather than the cause of genomic instability—our mouse model suggests Bap1 ablation suffices to cause defective DNA repair, pancreatitis, and cooperates with oncogenic Kras to promote pancreatic cancer."
BAP1 affects PRC1
| 2
| 2

sparser
"Recent CRISPR screening studies by Dixit’s group further identified that depletion of PRC1 subunit Ring1B (but not Ring1A) could rescue the cell death induced by loss of BAP1 xref , indicating that there is a robust and direct epigenetic dynamic occurring between the PRC1 and BAP1 complexes that require a balanced state to properly regulate gene expression and determine cell fate."

sparser
"Given that the composition and expression of PRC1 and BAP1 complexes changes extensively during development ( xref ; xref , xref ; xref ; xref ), in future work, it will be interesting to investigate how the balance between these two opposing activities is regulated at different developmental stages and how cell type-specific H2AK119ub1 levels influence the transcriptional potential of the genome."
BAP1 affects PDH
| 2
Mutated BAP1 phosphorylates PDH. 2 / 2
| 2

reach
"These findings suggest that further examination of PDH complex activity, detection of non-phosphorylated PDH, and the roles of elevated total PDH expression may be important to understand why BAP1 mutant UM cells has both increased total and phosphorylated PDH."

reach
"In addition, BAP1 mutant UM cells showed increased total and phosphorylated PDH levels compared to BAP1 wild type UM cells, while the expression of PDHK1 and DLAT remained unaltered (Fig. 2b and Supplementary Fig. 1b)."
BAP1 affects PCBD1
| 2
BAP1 inhibits PCBD1. 2 / 2
| 2

reach
"Overexpression of BAP1 or BAP2, with their partner BON1, inhibits PCD induced by pathogens."

reach
"Overexpression of BAP1 or BAP2 with their partner BON1 inhibits PCD induced by pathogens, the proapoptotic gene BAX, and superoxide generating paraquat in Arabidopsis or Nicotiana benthamiana."
BAP1 affects PBRM1, SETD2, and VHL
| 2
VHL binds SETD2, PBRM1, and BAP1. 2 / 2
| 2

sparser
"The present data indicated that VHL, PBRM1, SETD2 and BAP1 are not associated with OS in all stages of ccRCC."

sparser
"Unlike other cancer types that are found to have recurrent mutations in oncogenes [ xref – xref ], ccRCC tumors are mainly associated with somatic mutations in tumor suppressor genes such as VHL , PBRM1 , BAP1 and SETD2 [ xref – xref ]."
BAP1 affects PARP1
| 1
| 1

reach
"Poly(ADP-ribose) polymerase 1 (PARP1) interacts with and recruits BAP1 to damage sites, with BAP1 recruitment peaking after the DDB2 and XPC damage sensors."
BAP1 affects NP_828865
2 |
BAP1 binds NP_828865. 2 / 2
2 |

No evidence text available

No evidence text available
BAP1 affects NFkappaB
| 1 1
BAP1 activates NFkappaB. 2 / 2
| 1 1

sparser
"BaP1 activates NF-κB."

reach
"BaP1 activates NF-kappaB."

eidos
"In addition , loss of BAP1 has also been described to deplete mitochondrial proteins in pancreas cells137 ."

eidos
"In the pancreas , knockdown of Bap1 diminished mitochondrial protein levels ( e.g ."
BAP1 affects Mice
| 2
BAP1 inhibits Mice. 2 / 2
| 2

reach
"Deubiquitinase BAP1 regulates stability of BRCA1 protein and inactivates the NF-κB signaling to protect mice from sepsis-induced acute kidney injury."

reach
"We found that loss of BAP1 leads to the development of chronic pancreatitis in mice and is associated with poor prognosis in PDA patients."
BAP1 affects MTOR, and SETD2
| 2
SETD2 binds MTOR and BAP1. 2 / 2
| 2

sparser
"By comparing tumours which cluster with the cell lines based on CNAs with tumours that cluster away from the cell lines, we found that the tumours likely to be best represented by the cell lines carry hallmarks of aggressive disease, such as higher stage, higher grade, greater extent of CNAs, and more frequent mutations in genes such as SETD2, BAP1 and MTOR , which have been associated with more aggressive disease and poorer outcomes."

sparser
"These tumours also display a higher extent of copy number aberrations (mean fraction genome altered: 19% versus 12%), and more frequent mutations in genes such as BAP1 (12.3% versus 5.4%), SETD2 (12.7% versus 8.1%) and MTOR (6% versus 4.7%), which are associated with more aggressive disease (though only BAP1 has a statistically significant difference— P value 0.025, Fisher's exact test)."
BAP1 affects L3MBTL2
| 1 1
| 1 1

sparser
"By targeting atypical polycomb protein L3MBTL2, BAP1 interacts with and stabilizes L3MBTL2, co-occupying and maintaining H4K20me1 at target gene loci, such as EZH2 locus."

reach
"By targeting atypical polycomb protein L3MBTL2, BAP1 interacts with and stabilizes L3MBTL2, co-occupying and maintaining H4K20me1 at target gene loci, such as EZH2 locus."
BAP1 affects KDM6A, and KMT2C
| 2
KDM6A binds KMT2C and BAP1. 2 / 2
| 2

sparser
"We found that cancer hotspot mutations in the MLL3 amino terminal PHD repeats disrupt its association with the BAP1 complex and result in reduced MLL3 and UTX recruitment to enhancers misregulating the epigenetic chromatin state at these loci."

sparser
"Mutations within the MLL3 PHD domains abolish the interaction between the BAP1 tumor suppressor complex and MLL3-UTX, which leads to attenuation of enhancer activity."
BAP1 affects KDM5C, PBRM1, SETD2, and VHL
| 2
| 2

sparser
"The objective of this hypotheses-generating Radiogenomics study was to investigate associations between CT features of ccRCC and mutations of the genes VHL, PBRM1, SETD2, KDM5C , and BAP1."

sparser
"The identification of genetic mutations is clinically relevant because both an advanced stage and poor survival have been associated with VHL, PBRM1, BAP1, SETD2, and KDM5C mutations [ xref , xref – xref ]."
BAP1 affects JNK
| 2
BAP1 inhibits JNK. 2 / 2
| 2

reach
"All of these results further confirmed that BAP1 suppressed ICC cell proliferation, cell cycle progression, and invasion via inhibiting ERK1/2 and JNK/c-Jun signaling pathways."

reach
"Taken together, our data suggested that BAP1 negatively regulated the ERK1/2 and JNK/c-Jun signaling pathways to exert its tumor-suppressive functions in ICC."
BAP1 affects Interferon
| 1 1
| 1 1

reach
"Previously, we found that hypoxia-inducible factor 2α (HIF2α) and BAP1 activate interferon-stimulated gene factor 3 (ISGF3), a transcription factor activated by type I interferons and a tumor suppressor in ccRCC xenograft models."

eidos
"Our results indicate that BAP1 loss reduces type I interferon signaling , and reactivating this pathway may be a novel therapeutic strategy for treating ccRCC ."
BAP1 affects ITPR1
| 2
| 2

sparser
"Taken together, these results indicated that DIM could induce ferroptosis to exert anti-cancer effects via BAP1-IP3R axis, suggesting its effective therapeutic potential in gastric cancer."

sparser
"3,3'-Diindolylmethane induces ferroptosis by BAP1-IP3R axis in BGC-823 gastric cancer cells."
BAP1 affects IL1B
| 2
BAP1 increases the amount of IL1B. 2 / 2
| 2

reach
"This study showed that the snake venom metalloprotease (SVMP) BaP1 activates FLSs to produce PGE 2 by a mechanism dependent on COX-2, mPGES-1 and iPLA 2 s. BaP1 also induces IL-1beta release, which up-regulates the production of PGE 2 at a late stage of the stimulation."

reach
"Taken together, our findings show that the metalloprotease BaP1 directly activates FLSs to produce PGE by a COX-2-dependent mechanism involving the coupling of the inducible enzymes COX-2 and mPGES-1 and intracellular PLA s. BaP1 also induced release of IL-1β, which up-regulates the production of PGE at a later stage of the stimulation."
BAP1 affects IFNB1
| 1 1
BAP1 increases the amount of IFNB1. 2 / 2
| 1 1

sparser
"In UMRC6 cells, in which BAP1-WT and BAP1-C91G were expressed to a similar extent ( xref ), BAP1-WT induced IFNB1 expression, whereas BAP1-C91G did not ( xref )."

reach
"BAP1 enhanced the expression of IFN-β and stimulator of interferon genes (STING), both of which activate ISGF3."
BAP1 affects Histone_H3
| 2
BAP1 leads to the methylation of Histone_H3 at position 27. 1 / 1
| 1

reach
"BAP1 loss results in increased global trimethylation of histone H3 lysine 27 (H3K27me3), which is catalyzed by the polycomb repressive complex 2 enzyme EZH2."
BAP1 leads to the methylation of Histone_H3 on lysine. 1 / 1
| 1

reach
"BAP1 loss results in increased global trimethylation of histone H3 lysine 27 (H3K27me3), which is catalyzed by the polycomb repressive complex 2 enzyme EZH2."
BAP1 affects Histone_H2B
| 2
BAP1 deubiquitinates Histone_H2B. 2 / 2
| 2

reach
"Loss of Asx in flies led to an increase in Ub-H2A levels without influencing other chromatin marks (H3K4 me3, H3K27me3), and assays using purified proteins found Asx stimulates Calypso activity towards Ub-AMC, and that Asx and Calypso and the human orthologs BAP1 and ASXL1 deubiquitinate H2A but not H2B in reconstituted nucleosomes [XREF_BIBR]."

reach
"Subsequent WB using anti-HA and anti- Flag antibodies showed that the BAP1 WT, but not a MT, deubiquitinated H2B in vivo."
BAP1 affects HOXA genes
| 2
BAP1 activates HOXA genes. 2 / 2
| 2

reach
"We also examined whether ASXL1-MT and BAP1 upregulated posterior HOXA genes and IRF8 in human RUNX1-ETO cells by qRT-PCR."

reach
"ASXL1-MT and BAP1 induces upregulation of HOXA genes and IRF8."
BAP1 affects HDAC1
| 1 1
BAP1 inhibits HDAC1. 2 / 2
| 1 1

reach
"We find that BAP1 loss promotes a switch from HDAC2 to HDAC1."

eidos
"The BAP1 knockdown led to a decrease in HDAC2 but an increase in HDAC1 expression ."
| PMC
BAP1 affects HCFC1, and OGT
| 2
HCFC1 binds OGT and BAP1. 2 / 2
| 2

reach
"The fact that HCF-1 acts as an important modulator of cellular circadian rhythm [XREF_BIBR] raises the possibility that the OGT, HCF-1, and BAP1 complex interacts with the BMAL1, CLOCK, and PER complex to modulate circadian rhythm."

reach
"Within the BAP1/HCF-1/OGT complex, HCF1 is modified by O-GlcNacylation, the addition of single O-linked N-acetylglucosamine moiety to specific serine or threonine residues (50,51)."
BAP1 affects H3K36me3, and PBRM1
| 2
PBRM1 binds BAP1 and H3K36me3. 2 / 2
| 2

sparser
"We previously evaluated the prognostic impact of BAP1 and PBRM1 to define epigenetic subtypes of clear cell renal cell carcinoma.( xref , xref ) To examine the association of H3K36me3 with BAP1 and PBRM1 protein expression, we evaluated the expression of all 3 markers dichotomized as positive versus negative in clear cell renal cell carcinoma samples with available staining ( xref )."

sparser
"Association of H3K36me3 with Epigenetic Tumor Suppressors BAP1 and PBRM1."
BAP1 affects H2AK119Ub
| 2
BAP1 inhibits H2AK119Ub. 2 / 2
| 2

reach
"Loss of BAP1 increased the intensity of both H2AK119Ub and H3K27me3, and repressed the majority of DEG (66.2% downregulated vs. 33.8% upregulated, Fig. 6d)."

reach
"Consequently, H2AK119Ub can also be dynamically reversed by the BAP1 complex, an evolutionarily conserved multiprotein complex that functions as a general transcriptional activator."
BAP1 affects H2AC20, and Histone
| 2
| 2

sparser
"Emerging studies have demonstrated that dysregulations in BAP1 histone H2A deubiquitinase complex are collectively associated with pathogenesis in human SCLC."

sparser
"Mutations of ASXL1, encoding a component of the BAP1 histone H2A deubiquitinase complex, occur in human myeloid neoplasms and are uniformly associated with poor prognosis."

reach
"This indicates that BAP1 loss did not significantly augment genomic instability in our BNC mouse model."

reach
"Loss of Bap1 causes genomic instability."
BAP1 affects GEP, and class 2
| 2
BAP1 binds GEP and class 2. 2 / 2
| 2

sparser
"12,36,39 BAP1 mutation is associated with monosomy 3 or class 2 GEP tumors and impart poor survival."

sparser
"BAP1 is associated with monosomy 3, poor prognosis, and class 2 GEP tumors, while EIF1AX is associated with class 1 GEP tumors and good prognosis."
BAP1 affects Flag
| 1 1
| 1 1

sparser
"Moreover, we observed that Flag-ASXL1 interacted with endogenous HCF-1, FOXK1 and FOXK2 in BAP1 knockdown HEK293T cells (Fig.  xref G), and that Flag-BAP1 interacted with endogenous HCF-1, FOXK1, and FOXK2 in ASXL1 knockdown HEK293T cells (Fig.  xref H)."

reach
"Full-length BAP1 (1-729), the BAP1 N terminus (1-240) and the BAP1 C terminus (597-729) interacted with KLF5-3 x Flag (XREF_FIG)."
BAP1 affects FLS
| 2
BAP1 activates FLS. 2 / 2
| 2

sparser
"This study showed that the snake venom metalloprotease (SVMP) BaP1 activates FLSs to produce PGE 2 by a mechanism dependent on COX-2, mPGES-1 and iPLA 2 s."

sparser
"Taken together, our findings show that the metalloprotease BaP1 directly activates FLSs to produce PGE 2 by a COX-2-dependent mechanism involving the coupling of the inducible enzymes COX-2 and mPGES-1 and intracellular PLA 2 s."
BAP1 affects EP4 receptor
| 2
BAP1 increases the amount of EP4 receptor. 2 / 2
| 2

reach
"BaP1 also up-regulates EP4 receptor expression."

reach
"In addition, BaP1 up-regulates EP4 receptor expression."
| 2

reach
"However, our previous work showed that the stability of Ino80 is regulated by the ubiquitin-proteasome system and that BAP1 functions as a deubiquitinase that stabilizes Ino80 and thus promotes DNA replication."

reach
"BAP1 contributes to colon cancer cell proliferation by accelerating DNA replication and suppressing replication stress and concomitant apoptosis."
BAP1 affects DNA break repair
| 2
BAP1 activates DNA break repair. 2 / 2
| 2

eidos
"The literature suggests that BAP1 promotes DNA double-strand break repair and that its loss leads to homologous recombination repair defects , reminiscent of BRCA1 / 2 deficiency ."

eidos
"Although BAP1 stimulates DNA double-strand break repair , whether it functions in nucleotide excision repair ( NER ) is unknown ."
BAP1 affects DIDO1
| 1 1
| 1 1

sparser
"BAP1 binds to DIDO1 and stabilizes DIDO1 through de-ubiquitination."

reach
"BAP1 binds to DIDO1 and stabilizes DIDO1 through de-ubiquitination."
BAP1 affects DGC
| 2
BAP1 inhibits DGC. 2 / 2
| 2

reach
"As in NCI-H226 cells, 25 BAP1 expression suppressed the growth of our DGC cells, suggesting that BAP1 is a novel tumor suppressor candidate in DGC."

reach
"All 4 of the BAP1 somatic mutations found in EODGC-WES were associated with loss of heterozygosity, and BAP1 over-expression suppressed the growth of DGC cells (XREF_FIG)."
BAP1 affects DDIT3
| 1 1
BAP1 inhibits DDIT3.
| 1
BAP1 inhibits DDIT3. 1 / 1
| 1

eidos
"BAP1 repressed glucose deprivation-induced ER stress cell death by inhibiting transcription of two major UPR-TFs , activating transcription factor 3 ( ATF3 ) and C / EBP homologous protein ( CHOP ) ( 70 ) ."
BAP1 binds DDIT3.
| 1
DDIT3 binds ATF3 and BAP1. 1 / 1
| 1

sparser
"Mechanically, we show that BAP1 represses metabolic stress-induced UPR and cell death through activating transcription factor 3 (ATF3) and C/EBP homologous protein (CHOP), and reveal that BAP1 binds to ATF3 and CHOP promoters and inhibits their transcription."
BAP1 affects Collagen
| 2
| 2

reach
"In the ex vivo model, BaP1 was able to degrade type IV collagen and laminin from the BM of microvessels."

reach
"In addition, BaP1 did not inhibit collagen induced platelet aggregation in vitro."

eidos
"BAP1 participates in homologous recombination ( HR ) repair and prevents chromosomal instability through modulation of various substrates , including MCRS1 and INO80 ."

reach
"BAP1 participates in homologous recombination (HR) repair and prevents chromosomal instability through modulation of various substrates, including MCRS1 and INO80."
BAP1 affects CTNNB1
| 2
BAP1 activates CTNNB1. 2 / 2
| 2

reach
"Mechanistically, PVT1 binds with KLF5 and increases its stability via BAP1, which upregulates beta-catenin signaling, resulting in enhanced TNBC tumorigenesis."

reach
"BAP1 depletion caused a reduction in mRNA levels of neural crest migration genes (ROBO1), melanocyte differentiation genes (CTNNB1, EDNRB and SOX10) and other genes that are down-regulated in class 2 tumors (LMCD1 and LTA4H)."
BAP1 affects CDH1
| 2
BAP1 decreases the amount of CDH1. 2 / 2
| 2

reach
"BAP1 re-expression in UM cells reduced E-cadherin and CADM1 levels."

reach
"BAP1 re-expression in UM cells reduced E-cadherin and CADM1 levels."
BAP1 affects CD3EAP
| 1 1
| 1 1

sparser
"BAP1 interacts with CAST in UM cells, and CAST and its subsequent calpain pathway may mediate BAP1-related cell migration regulation."

reach
"BAP1 interacts with CAST in UM cells, and CAST and its subsequent calpain pathway may mediate BAP1 related cell migration regulation."
BAP1 affects CCDC6
| 2
| 2

sparser
"Coiled-coil domain containing 6 (CCDC6) interacts with BAP1 and has been reported to regulate both homologous recombination and PARP inhibitor sensitivity."

sparser
"The interaction between CCDC6 and BAP1 was confirmed in MPM cells, by co-immunoprecipitation."
BAP1 affects CADM1
| 2
BAP1 decreases the amount of CADM1. 2 / 2
| 2

reach
"BAP1 re-expression in UM cells reduced E-cadherin and CADM1 levels."

reach
"BAP1 re-expression in UM cells reduced E-cadherin and CADM1 levels."
BAP1 affects BRCA1, and RING
| 2
BRCA1 binds BAP1 and RING. 2 / 2
| 2

sparser
"BAP1 interacts with the RING domain of human BRCA1, a predominately nuclear, ovarian and breast cancer susceptibility gene product with tumor suppressor activity."

sparser
"The BRCA1 RING domain also binds BAP1 (BRCA1-associated protein-1), a de-ubiquitinating enzyme 26 ."
BAP1 affects BRCA
| 2
| 2

reach
"We assessed whether tumour BRCA associated protein 1 (BAP1) status is a predictive biomarker for survival in patients receiving first-line combination platinum/pemetrexed therapy.introduction METHODS: PM cases (n=114) from Aalborg, Denmark were stained for BAP1 on tissue microarrays."

reach
"Recent work has shown that BRCA-associated protein 1 (BAP1), which functions as part of the polycomb repressive deubiquitinase complex that removes monoubiquitination on H2AK119, regulates SLC7A11, a subunit of the heterodimeric system x cystine-glutamate antiporter."
BAP1 affects BCR
| 2
| 2

reach
"Likewise, the mutants carrying box-1 (residues 166-208) of the tau-isoform, a rat counterpart of mouse Bcr binding protein Bap-1 [11], bound Bcr, while the mutants lacking box-1 did not.To further eva[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As shown in Figure 5, tyrosine phosphorylation of Bcr by Fes greatly reduced Bcr binding to Bap-1."
BAP1 affects ATP
| 2
BAP1 inhibits ATP. 2 / 2
| 2

reach
"BAP1 +/- fibroblasts displayed increased aerobic glycolysis and lactate secretion, and reduced mitochondrial respiration and ATP production compared with BAP1 WT."

reach
"BAP1 inhibits glucose deprivation induced reactive oxygen species and ATP depletion, two cellular events contributing to the ER stress induced cell death."
BAP1 affects ATM
| 1 1
| 1 1

reach
"8 BAP1 also interacts with ataxia telangiectasia mutated and ataxia telangiectasia and Rad3 related proteins, which regulate the DNA damage induced by UV radiation."

sparser
"Further large germline studies of biliary tract cancers are warranted to better assess the association of ATM and BAP1 with this rare cancer type."
BAP1 affects ASXH domain
| 2
ASXL1 binds BAP1 and ASXH domain. 2 / 2
| 2

reach
"As mentioned earlier, the interaction between BAP1 and ASXL1 occurs through the ASXH domain."

reach
"ASXL1 directly binds to BAP1 through the ASXH domain and its adjoining region."
BAP1 affects ANKRD17
2 |
2 |

No evidence text available

No evidence text available
BAP1 affects 6-dEB
| 2
BAP1 activates 6-dEB. 2 / 2
| 2

reach
"It has been found that BAP1 could produce 6-dEB in the absence of propionate and methylmalonate."

reach
"BAP1 produced 6-dEB at a titer of 0.32 +/- 0.11 mg l -1 from 20 mM methylmalonate (XREF_FIG)."
ASXL2 affects YUH1
| 2
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
ASXL2 affects BAP1, ULD, and ab
| 2
ASXL2 binds BAP1, ab, and ULD. 2 / 2
| 2

sparser
"These data are consistent with our previous studies that used computer modeling technology to predict the molecular model of BAP1-ULD interacting with ASXL2-AB ( xref )."

sparser
"Biochemical analyses of purified recombinant protein complexes from bacteria reveal a direct interaction between the BAP1-ULD domain and ASXL2-AB box."
ASXL2 affects BAP1, ULD, YUH1, and ab
| 2
ASXL2 binds BAP1, YUH1, ab, and ULD. 2 / 2
| 2

sparser
"Instead, the BAP1 ULD domain binds to the AB box, which leads to the formation of a stable ternary complex consisting of the BAP1-UCH, BAP1-ULD and ASXL2-AB domains ( xref – xref )."

sparser
"The peak fraction of the AB box in the complex shifted dramatically from #17 to #15 compared to AB alone, supporting our hypothesis that BAP1-UCH, BAP1-ULD and ASXL2-AB form a ternary stable complex in vitro and that ULD recruits ASXL2-AB to access the BAP1-UCH catalytic motif."
ASXL1 affects PTEN
| 2
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
ASXL1 affects PGR
| 2
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
ASXL1 affects BAP1, and PTEN
| 2
ASXL1 binds BAP1 and PTEN. 2 / 2
| 2

sparser
"Then we performed ChIP-qPCR analyses in 32D cells to validate whether Asxl1 and Bap1 are bound to the Pten promoter and examined the accompanying changes of histone modifications in different conditions."

sparser
"As shown in xref , Asxl1 and Bap1 are specifically bound to the Pten promoter, and their enrichment is further induced by the absence of IL3 in WT cells."
ASXL1 affects BAP1, and PGR
| 2
ASXL1 binds PGR and BAP1. 2 / 2
| 2

sparser
"The K119 Ub-H2A is removed by the deubiquitinase complex PR-DUB (Polycomb Repressive-Deubiquitinase complex) formed by the proteins ASXL1 (Additional Sex Combs like 1) and BAP1 (BRAC1 Associated Protein 1) [ xref ]."

sparser
"Like Drosophila Calypso and ASX, human BAP1 and ASXL1 proteins could thus also be assembled into a stable PR-DUB complex ( xref , right, lane 8), demonstrating the evolutionary conservation of this interaction."
ASXL1 affects ASXH domain
| 2
ASXL1 binds BAP1 and ASXH domain. 2 / 2
| 2

reach
"As mentioned earlier, the interaction between BAP1 and ASXL1 occurs through the ASXH domain."

reach
"ASXL1 directly binds to BAP1 through the ASXH domain and its adjoining region."
ANKRD17 affects BAP1
2 |
2 |

No evidence text available

No evidence text available
AM146 affects BAP1
| 2
AM146 inhibits BAP1. 2 / 2
| 2

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14, which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7."

reach
"UCHL1, UCHL3, USP2 and USP8 were found to be inhibited by AM146, RA-9, and RA-14 which did not inhibit Ataxin-3, A20, BAP1, Otubain 1 or USP7 [XREF_BIBR]."
ABL1 affects BAP1
| 1 1
ABL1 phosphorylates BAP1 on tyrosine. 2 / 2
| 1 1

rlimsp
"Bap-1 is a substrate for the Bcr serine-threonine kinase and is also phosphorylated on tyrosine by Bcr-Abl but not by c-Abl."

reach
"Bap-1 is a substrate for the Bcr serine threonine kinase and is also phosphorylated on tyrosine by Bcr-Abl but not by c-Abl."
| 2

reach
"Global DNA methylation was analyzed in 47 Class 1 and 45 Class 2 primary UMs and in UM cells engineered to inducibly deplete BAP1."

reach
"The peculiar UM proneness in MBD4-mutant carriers (13 out of 23 carriers; XREF_TABLE) remains unexplained, but the fact that the frequent M3 in UM inactivates wild-type copies of both BAP1 and MBD4 suppressor genes may at least in part explain the frequent inactivation of MBD4 in UM."
| 1

sparser
"BAP1 and ASXL proteins form mutually exclusive complexes of the PR-DUB tumour suppressor, which maintains transcriptional silencing of polycomb target genes."
SiPDHK1 affects BAP1
| 1
SiPDHK1 leads to the phosphorylation of mutated BAP1. 1 / 1
| 1

reach
"As expected, knockdown of PDHK1 (siPDHK1) also significantly decreased phosphorylation of PDH and BAP1 mutant MP65 cell growth (Fig. 5c)."

reach
"BAP1 expression may serve as a biomarker of radioresponse, and further investigation of the interaction between BAP1, HR, and radiation in this disease is warranted."
RebG affects BAP1
| 1
| 1

reach
"Major components of the V. cholerae biofilm are Vibrio polysaccharide (VPS), which is produced by proteins encoded in the vps-I and vps-II gene clusters, as well as matrix proteins (RbmA, RbmC, and Bap1) that facilitate cell-cell and cell-surface interactions and contribute to biofilm architecture [XREF_BIBR - XREF_BIBR]."
Protein affects BAP1
| 1
Protein activates BAP1. 1 / 1
| 1

eidos
"ASXL proteins activate BAP1 by forming the polycomb repressive deubiquitinase ( PR-DUB ) complex which acts on H2AK119ub1 ."
Monomethylarsonous acid increases the amount of BAP1. 1 / 1
1 |

No evidence text available
MTORC1 affects BAP1
| 1
| 1

sparser
"PBRM1 and BAP1 likely affect different epigenetic programs, and BAP1 loss is associated with high grade and mTORC1 activation."
Hsa-miR-4489 affects BAP1
1 |
Hsa-miR-4489 decreases the amount of BAP1. 1 / 1
1 |

No evidence text available
Hsa-miR-4283 affects BAP1
1 |
Hsa-miR-4283 decreases the amount of BAP1. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-1229-3p decreases the amount of BAP1. 1 / 1
1 |

No evidence text available
Folic acid affects BAP1
1 |
Folic acid methylates BAP1. 1 / 1
1 |

No evidence text available
Extrascleral extension affects BAP1
| 1
Extrascleral extension inhibits BAP1. 1 / 1
| 1

eidos
"Lower PPARG levels also correlated with an increased tumor thickness , advanced clinical stage , extrascleral extension , reduced BAP1 expression , epithelioid structure , and an adverse prognosis ."

sparser
"Investigating the cytoplasmic function of BAP1, the authors focused on the possible interaction between BAP1 and ER channels that control Ca 2+ flux to the cytoplasm."
1 |
1 |

No evidence text available
CKO affects BAP1
| 1
| 1

sparser
"Based on their findings, BAP1-cKO mice leads to the development of splenomegaly within 4 weeks of BAP1 depletion, and myeloid cells were significantly increased in lymph nodes and bone marrow in BAP1-cKO mice, resulting in myeloid transformation xref ."
| 1

reach
"Since VPS contains these sugars, it is possible that Bap1 and RbmC may bind to the galactose, mannose and/or glucose residues of VPS."
ZC3H13 affects BAP1
1 |
1 |

No evidence text available
ZAT10 affects BAP1
| 1
ZAT10 activates BAP1. 1 / 1
| 1

reach
"In LTC primed plants, higher light intensity and cold blocked ZAT10 induction similarly and elevated light blocked BAP1 even stronger."
TSNAX affects BAP1
1 |
1 |

No evidence text available
TFCP2 affects BAP1
1 |
TFCP2 decreases the amount of BAP1. 1 / 1
1 |

No evidence text available
SETD2 affects KMT2D
| 1
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
SEMA3F affects BAP1
| 1
SEMA3F binds BAK1 and BAP1. 1 / 1
| 1

sparser
"TNK2 is known to encourage prostate tumorigenesis, xref – xref Some of the down-regulated genes, like SEMA3F , BAK1 , and BAP1 are associated with tumor suppression. xref , xref – xref Many of the down-regulated genes are also associated with decreased invasion and motility, such as DDR1 , ZYX , NES , and PLEC . xref – xref "
RP23-49C8.1 affects BAP1
1 |
BAP1 binds RP23-49C8.1. 1 / 1
1 |

No evidence text available

eidos
"BAP1 downregulation by siRNA inhibited apoptosis induced by the combined treatment of ODN and oxaliplatin / etoposide ."
PI3K affects BAP1
| 1
PI3K inhibits BAP1. 1 / 1
| 1

reach
"BAP1 isoform lacking part of the catalytic domain sensitized MPM cells to the PARP1 inhibitor, olaparid, and this sensitivity could be augmented by concomitant treatment with apitolisib (GDC-0980), a dual inhibitor of class 1 PI3K, and mTOR kinase, which downregulates BRCA-1."
| PMC
PGR affects FOXK2
| 1
FOXK2 binds PGR and BAP1. 1 / 1
| 1

sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
PDC affects BAP1
| 1
| 1

sparser
"In addition, KMT2C was shown to bind to the tumor suppressor BAP1 through its PHD domain in cancer cells, and disruption of this interaction via KMT2C mutations led to impaired KMT2C and UTX recruitment to enhancers and increased cancer cell proliferation [ xref ]."
PBRM1 affects KMT2D
| 1
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
OCM affects CM
| 1
OCM binds BAP1 and CM. 1 / 1
| 1

sparser
"In conclusion, our evidence suggests that germline BAP1 inactivation is preferentially associated with metastatic OM and hereditary CM-OM in a small but significant proportion of cases."
Notch affects BAP1
| 1
| 1

reach
"Prompted by the upregulation of Notch pathway members in metastasis-prone class 2 tumors and the association between Notch and BAP1 in normal development, we examined whether Notch activity was required in uveal melanoma."
NUP214 affects BAP1
| 1
| 1

reach
"When tagging BAP1 for AP-MS experiments, the tagged protein can either form a dimer with another tagged BAP1, or an endogenous BAP1 protein."
| PMC
Mutation affects BAP1
| 1
| 1

eidos
"Alternatively , BAP1 deficiency may not only be induced by mutations ."
MYC affects BAP1
1 |
MYC decreases the amount of BAP1. 1 / 1
1 |

No evidence text available
MVD affects BAP1
| 1
| 1

sparser
"Concluding, vascular density of UM relates to its genetic profile: Monosomy 3 and BAP1-loss are associated with an increased MVD, while an early event (gain of 8q) is not independently related to MVD, but may initiate a preparation phase towards development of vessels."
KMT2D affects SETD2
| 1
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
H2AK119 affects BAP1
| 1
H2AK119 activates BAP1. 1 / 1
| 1

reach
"In the absence of BAP1 in certain cell types, the ubiquitin ligase RING1B (RNF2)in polycomb complex 1 subtypes promotes apoptosis by ubiquitination of H2AK119 on Bcl2 and Mcl1 prosurvival genes, resulting in repression of these survival factors [12]."
| PMC
Gon4l/Udu affects BAP1
| 1
BAP1 binds Gon4l/Udu. 1 / 1
| 1

sparser
"Based on these data and the newly identified interaction between Bap1 and Gon4l/Udu, Bap1 may provide a key connection between Gon4l/Udu and DDR."
FOXK2 affects PGR
| 1
FOXK2 binds PGR and BAP1. 1 / 1
| 1

sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
ELF2 affects BAP1
1 |
1 |

No evidence text available
E2/E3 affects BAP1
| 1
E2/E3 ubiquitinates BAP1. 1 / 1
| 1

reach
"Thus, substrate ubiquitination by UBE2O can induce cytoplasmic localization, which might be accompanied by further proteasomal degradation as a consequence of ubiquitin chain extension by other E3 lig[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
DUB inhibitor affects BAP1
| 1
DUB inhibitor activates BAP1. 1 / 1
| 1

reach
"b-AP15 is not an indiscriminate DUB inhibitor, since in isolated DUB assays it does not inhibit USP2/7/8, UCHL1, UCHL3 or BAP1 at 50 μM. Even though 19S inhibitory potency was modest, the Linder group showed that 37 inhibited tumour progression in four different in vivo solid tumour models in mice (2.5-5 mg/kg by subcutaneous injection), validating the 19S regulatory particle as a new anticancer drug target."
DNASE1 affects BAP1
| 1
DNASE1 activates BAP1. 1 / 1
| 1

reach
"RNA from BAP1 knock-down and out cells were treated with DNase I and 2mug of DNase treated RNA was reverse transcribed."
DDIT3 affects BAP1
| 1
DDIT3 binds ATF3 and BAP1. 1 / 1
| 1

sparser
"Mechanically, we show that BAP1 represses metabolic stress-induced UPR and cell death through activating transcription factor 3 (ATF3) and C/EBP homologous protein (CHOP), and reveal that BAP1 binds to ATF3 and CHOP promoters and inhibits their transcription."
Cyclin affects BAP1
| 1
Cyclin activates BAP1. 1 / 1
| 1

reach
"XREF_BIBR A hereditary predisposition to melanoma is most commonly caused by a heterozygous germline mutation in cyclin dependent kinase (CDK) gene CDKN2A and, to a lesser extent, CDK4, BRCA1 associated protein 1, MITF, and telomerase reverse transcriptase genes."
CM affects OCM
| 1
OCM binds BAP1 and CM. 1 / 1
| 1

sparser
"In conclusion, our evidence suggests that germline BAP1 inactivation is preferentially associated with metastatic OM and hereditary CM-OM in a small but significant proportion of cases."
CCR5 affects BAP1
| 1
| 1

reach
"The association between BAP1 and CCR5 or its ligands was assessed by immunohistochemistry, flow cytometry, real-time PCR and ELISA."
CAMK2D affects BAP1
1 |
1 |

No evidence text available
BRD4 affects BAP1
| 1
| 1

sparser
"In HEK293T cells, which express very low levels of ASXL3, transfection with GFP-tagged ASXL3 showed a dose-dependent increase in the protein-protein interaction between BAP1 and BRD4 (Additional file xref : Figure S2I)."
| 1

sparser
"BAP1 and ASXL proteins form mutually exclusive complexes of the PR-DUB tumour suppressor, which maintains transcriptional silencing of polycomb target genes."
BAP1 affects tumor development
| 1
BAP1 inhibits tumor development. 1 / 1
| 1

eidos
"BAP1 loss accelerates tumor development in a mouse model of mesothelioma ."
BAP1 affects tamoxifen
| 1
| 1

reach
"This screen, along with subsequent validation experiments, identifies a compendium of genes whose silencing causes tamoxifen resistance (including BAP1, CLPP, GPRC5D, NAE1, NF1, NIPBL, NSD1, RAD21, RARG, SMC3, and UBA3) and also a set of genes whose silencing causes sensitivity to this endocrine agent (C10orf72, C15orf55 and NUT, EDF1, ING5, KRAS, NOC3L, PPP1R15B, RRAS2, TMPRSS2, and TPM4)."
BAP1 affects sites
| 1
BAP1 inhibits sites. 1 / 1
| 1

eidos
"Poly ( ADP-ribose ) polymerase 1 ( PARP1 ) interacts with and recruits BAP1 to damage sites , with BAP1 recruitment peaking after the DDB2 and XPC damage sensors ."
BAP1 affects sensitivity radiation
| 1
BAP1 inhibits sensitivity radiation. 1 / 1
| 1

eidos
"Interestingly , we found that inhibition of BAP1 universally increased sensitivity to radiation in all cell line variants tested and that forced expression of BAP1 WT reversed this phenomenon ( Fig. 2 ) ."
BAP1 affects reprogramming cell metabolism
| 1
BAP1 inhibits reprogramming cell metabolism. 1 / 1
| 1

eidos
"Moreover , BAP1 deficiency drives the reprogramming of cell metabolism , promoting anaerobic glycolysis for energy production rather than mitochondrial respiration and increasing extracellular lactate secretion which induces immune evasion , tumour growth and cell malignant transformation ."
| 1

eidos
"We previously reported that the stability of Ino80 , the catalytic ATPase subunit of INO80 , is regulated by the ubiquitin proteasome system and that BRCA1-associated protein-1 ( BAP1 ) , a nuclear deubiquitinase with tumor suppressor activity , stabilizes Ino80 via deubiquitination and promotes replication fork progression ."

reach
"BAP1 expression may serve as a biomarker of radioresponse, and further investigation of the interaction between BAP1, HR, and radiation in this disease is warranted."
BAP1 affects rebG
| 1
| 1

reach
"Major components of the V. cholerae biofilm are Vibrio polysaccharide (VPS), which is produced by proteins encoded in the vps-I and vps-II gene clusters, as well as matrix proteins (RbmA, RbmC, and Bap1) that facilitate cell-cell and cell-surface interactions and contribute to biofilm architecture [XREF_BIBR - XREF_BIBR]."
BAP1 affects phosphorylation ERK1 JNK
| 1
BAP1 inhibits phosphorylation ERK1 JNK. 1 / 1
| 1

eidos
"More importantly , our results also confirmed that BAP1 deficiency increased the phosphorylation and activity of ERK1 / 2 and JNK / c-Jun signaling to promote ICC progression ."
BAP1 affects phenotypical evolution WDPM
| 1
BAP1 inhibits phenotypical evolution WDPM. 1 / 1
| 1

eidos
"Well-differentiated papillary mesothelioma ( WDPM ) with BAP1 loss progresses to MPM in the long term , showing that BAP1 loss may induce phenotypical evolution of WDPM ."
BAP1 affects pathogenesis human cancers
| 1
BAP1 activates pathogenesis human cancers. 1 / 1
| 1

eidos
"However , it has not yet been elucidated whether BAP1 modulates the pathogenesis and progression of human cancers through some common cellular and molecular mechanisms , and a pan-cancer analysis for the roles of BAP1 has not yet been conducted ."
BAP1 affects nuclear export134
| 1
BAP1 activates nuclear export134. 1 / 1
| 1

eidos
"However , BAP1 can be ubiquitylated by ubiquitin-conjugating enzyme E2 O ( UBE2O ) at its NLS to promote nuclear export134 ."
BAP1 affects multinucleation
| 1
BAP1 inhibits multinucleation. 1 / 1
| 1

eidos
"Similarly , BAP1 depletion induced multipolar spindle formation and multinucleation , which were partially rescued by the stable overexpression of MCRS1 in RCC cells147 ."
BAP1 affects mesenchymal-epithelial transition kidney tumor cells
| 1
BAP1 inhibits mesenchymal-epithelial transition kidney tumor cells. 1 / 1
| 1

eidos
"For BAP1 , it had been proven that BAP1 served as a tumor suppressor , and the loss of BAP1 could result in enhanced mesenchymal-epithelial transition in kidney tumor cells [ 17 , 18 ] ."
BAP1 affects interaction
| 1
BAP1 activates interaction. 1 / 1
| 1

eidos
"PVT1 binds KLF5 , an interaction that is enhanced by BAP1 ( BRCA1-associated protein ) , to upregulate beta-catenin signaling and promote TNBC tumorigenesis [ 237 ] ."
| PMC
| 1

reach
"Results of these assays suggest that glycolic acid interacts directly with BaP1 and chelates the Zn (2) + ion at the active site."
BAP1 affects glucose deprivation-induced apoptosis
| 1
BAP1 inhibits glucose deprivation-induced apoptosis. 1 / 1
| 1

eidos
"In RCC cells , BAP1 inhibited glucose deprivation-induced apoptosis through interaction with the activating transcription factor 3 ( ATF3 ) and DNA damage-inducible transcript 3 protein ( DDIT3 ) gene promoters in a polycomb complex protein BMI-1-dependent manner136 ."
BAP1 affects deubiquitylase adaptor module DEUBAD
| 1
BAP1 deubiquitinates deubiquitylase adaptor module DEUBAD. 1 / 1
| 1

reach
"Furthermore, BAP1 deubiquitylates the deubiquitylase adaptor module DEUBAD of ASXL2, leading to its stabilization."
BAP1 affects casein
| 1
BAP1 inhibits casein. 1 / 1
| 1

reach
"P-I metalloproteinases (BaP1) isolated from B. asper degraded the casein at 17.8 units/mg protein [44]."
BAP1 affects cKO
| 1
| 1

sparser
"Based on their findings, BAP1-cKO mice leads to the development of splenomegaly within 4 weeks of BAP1 depletion, and myeloid cells were significantly increased in lymph nodes and bone marrow in BAP1-cKO mice, resulting in myeloid transformation xref ."
BAP1 affects anaerobic glycolysis energy
| 1
BAP1 inhibits anaerobic glycolysis energy. 1 / 1
| 1

eidos
"Moreover , BAP1 deficiency drives the reprogramming of cell metabolism , promoting anaerobic glycolysis for energy production rather than mitochondrial respiration and increasing extracellular lactate secretion which induces immune evasion , tumour growth and cell malignant transformation ."
| 1

reach
"Since VPS contains these sugars, it is possible that Bap1 and RbmC may bind to the galactose, mannose and/or glucose residues of VPS."
BAP1 affects activation cysteine-dependent cell death
| 1
BAP1 activates activation cysteine-dependent cell death. 1 / 1
| 1

eidos
"In addition to apoptosis , BAP1 also modulates the activation of cysteine-dependent cell death , ferroptosis , by downregulating the expression of SLC7A11 , the major transporter for extracellular cysteine uptake ."
BAP1 affects ZC3H13
1 |
1 |

No evidence text available
BAP1 affects VIM
| 1
BAP1 decreases the amount of VIM. 1 / 1
| 1

reach
"Using patient derived lymphoblastoid cell lines, we found that carriers of pathogenic BAP1 germline variants (c. 852_del and c. 1358_1359del) had reduced expression of full-length BAP1, Vimentin and Snail, as compared to controls with wild-type BAP1 (BAP1 WT), whereas BAP1 germline VUS (c. 299T> C and c. 551A> G) or likely benign carriers were not significantly different from wild-type BAP1 WT."
BAP1 affects UM metastasis
| 1
BAP1 activates UM metastasis. 1 / 1
| 1

eidos
"However , the molecular mechanisms through which BAP1 promotes UM metastasis is still unclear ."
BAP1 affects TSNAX
1 |
1 |

No evidence text available

reach
"In small cell lung carcinoma (SCLC), BAP1 promotes oncogenic roles inducing the expression of ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1), a key lineage-specific oncogenic driver in SCLC."
BAP1 affects SEMA3F
| 1
SEMA3F binds BAK1 and BAP1. 1 / 1
| 1

sparser
"TNK2 is known to encourage prostate tumorigenesis, xref – xref Some of the down-regulated genes, like SEMA3F , BAK1 , and BAP1 are associated with tumor suppression. xref , xref – xref Many of the down-regulated genes are also associated with decreased invasion and motility, such as DDR1 , ZYX , NES , and PLEC . xref – xref "
BAP1 affects S phase OCM1 cells NCI-H226 lung carcinoma cell line
| 1
BAP1 activates S phase OCM1 cells NCI-H226 lung carcinoma cell line. 1 / 1
| 1

eidos
"Moreover , BAP1 knockdown leads to G1 arrest and decreases the expression of S phase genes in OCM1 cells and NCI-H226 lung carcinoma cell line ( 47 , 48 , 80 ) ."
BAP1 affects RP23-49C8.1
1 |
BAP1 binds RP23-49C8.1. 1 / 1
1 |

No evidence text available
BAP1 affects Protease
| 1
BAP1 deubiquitinates Protease. 1 / 1
| 1

reach
"Downstream of ASXN is the ASX homology (ASXH) domain (also known as the DEUBAD domain) encoded by exons 9-11, which participates in interactions with epigenetic regulatory proteins, including the BRCA1 Associated Protein 1 (BAP1) deubiquitinating protease [XREF_BIBR - XREF_BIBR]."
BAP1 affects PDC
| 1
| 1

sparser
"In addition, KMT2C was shown to bind to the tumor suppressor BAP1 through its PHD domain in cancer cells, and disruption of this interaction via KMT2C mutations led to impaired KMT2C and UTX recruitment to enhancers and increased cancer cell proliferation [ xref ]."
BAP1 affects Notch
| 1
| 1

reach
"Prompted by the upregulation of Notch pathway members in metastasis-prone class 2 tumors and the association between Notch and BAP1 in normal development, we examined whether Notch activity was required in uveal melanoma."
BAP1 affects NUP214
| 1
| 1

reach
"When tagging BAP1 for AP-MS experiments, the tagged protein can either form a dimer with another tagged BAP1, or an endogenous BAP1 protein."
| PMC
BAP1 affects NRPSs
| 1
BAP1 activates NRPSs. 1 / 1
| 1

reach
"On the other hand, the E. coli BAP1 strain carrying a chromosomal Sfp gene is able to produce several functional cyanobacterial FASs, PK synthases (PKSs) and NRPSs XREF_BIBR - XREF_BIBR, indirectly suggesting the versatility of Sfp in activating cyanobacterial CPs."
BAP1 affects NFE2L2
| 1
BAP1 decreases the amount of NFE2L2. 1 / 1
| 1

reach
"Lentiviral transduction of BAP1 decreased the expression of NRF2 target genes, suppressed the migration and invasion, and sensitized cisplatin-induced apoptosis in human lung adenocarcinoma (LUAD) A549 cells."
BAP1 affects MVD
| 1
| 1

sparser
"Concluding, vascular density of UM relates to its genetic profile: Monosomy 3 and BAP1-loss are associated with an increased MVD, while an early event (gain of 8q) is not independently related to MVD, but may initiate a preparation phase towards development of vessels."
BAP1 affects MAPK
| 1
BAP1 inhibits MAPK. 1 / 1
| 1

reach
"BAP1, a tumor suppressor gene that functions in the BRCA1 growth pathway [XREF_BIBR]; HIF1AN, inhibits HIF1A transcriptional activity [XREF_BIBR]; MAPK transduction protein such as MAP4K5; RAS homologues RAB14, RAB6B, and RASA1 were found as potential targets of miR-31."
BAP1 affects KMT2D
| 1
| 1

sparser
"Several recent studies have shown that, in cc RCC, in addition to inactivating mutations in VHL tumor suppressor genes, genes such as BAP1, PBRM1, SETD2, and KDM5C play a role in tumor development, for example, causing mutations in the ubiquitin-mediated protein hydrolysis pathway (UMPP) in cc RCC; recent sequencing studies of cc RCC sequencing studies have recently identified chromatin modification genes such as PBRM1, KDM6A/UTX, KDM5C/JARID1C, SETD2, MLL2, and BAP1 that are highly associated with inactivating point mutations in their progression and poor prognosis."
BAP1 affects KLF5-mediated growth melanoma
| 1
BAP1 activates KLF5-mediated growth melanoma. 1 / 1
| 1

eidos
"Besides , BAP1 promoted KLF5-mediated growth of melanoma in vivo ."
BAP1 affects KIRC
| 1
BAP1 activates KIRC. 1 / 1
| 1

reach
"Besides, a study of families with BAP1 germline mutations shows that mutated BAP1 may promote oncogenesis and increase the risk of KIRC by 8-fold through altering cellular metabolism and the tumor microenvironment (25)."
BAP1 affects ISGF3
| 1
Modified BAP1 increases the amount of ISGF3. 1 / 1
| 1

reach
"Moreover, loss of SETD2 or BAP1 also reduces the ISGF3 level."
BAP1 affects HOXA gene
| 1
BAP1 decreases the amount of HOXA gene. 1 / 1
| 1

reach
"Importantly, BAP1 depletion inhibits posterior HOXA gene expression and leukaemogenicity of ASXL1-MT-expressing myeloid leukemia cells."
BAP1 affects H2AK119
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BAP1 deubiquitinates H2AK119. 1 / 1
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"28 ASXL1 may also collaborate with BAP1, loss of which led to a myelodysplastic syndrome like syndrome in a mouse model, to deubiquitinate H2AK119 at polycomb group targets."
BAP1 affects Gon4l/Udu
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BAP1 binds Gon4l/Udu. 1 / 1
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sparser
"Based on these data and the newly identified interaction between Bap1 and Gon4l/Udu, Bap1 may provide a key connection between Gon4l/Udu and DDR."
BAP1 affects FOXK2, and PGR
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FOXK2 binds PGR and BAP1. 1 / 1
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sparser
"FOXK2 binds to the BAP1-containing PR-DUB complex."
BAP1 affects ELF2
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No evidence text available
BAP1 affects DSB repair
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BAP1 activates DSB repair. 1 / 1
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eidos
"In conclusion , BAP1 deficiency might lead to impaired DSB repair by HR and could potentially increase the reliance on parallel repair pathways in a similar manner as BRCA1 deficiency ."
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"In addition, BAP1 mutations also cause basal cell carcinoma (BCC, 6.3% frequency) and squamous cell carcinoma (SCC, 1.1% frequency) in BAP1 carriers (11)."
BAP1 affects CYCD1-1
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BAP1 increases the amount of CYCD1-1. 1 / 1
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"In addition, expression of KLF5, CyclinD1, and FGF-BP1 was increased by BAP1 overexpression and decreased by BAP1 knockdown."
BAP1 affects CM, and OCM
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OCM binds BAP1 and CM. 1 / 1
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"In conclusion, our evidence suggests that germline BAP1 inactivation is preferentially associated with metastatic OM and hereditary CM-OM in a small but significant proportion of cases."
BAP1 affects CASP14
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BAP1 inhibits CASP14. 1 / 1
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"Kdm6a, Kmt2c, Asxl2, Bap1, Setd2 and Apc Suppress Breast Cancer in Mice."
BAP1 affects CAMK2D
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No evidence text available
BAP1 affects BRD4
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sparser
"In HEK293T cells, which express very low levels of ASXL3, transfection with GFP-tagged ASXL3 showed a dose-dependent increase in the protein-protein interaction between BAP1 and BRD4 (Additional file xref : Figure S2I)."
BAP1 affects BNC1
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BAP1 inhibits BNC1. 1 / 1
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"This indicates that BAP1 loss did not significantly augment genomic instability in our BNC mouse model."
BAP1 affects BCL2L11
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BAP1 inhibits BCL2L11. 1 / 1
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"The tumour was histopathologically atypical because of the presence of confluent pleomorphism, solid sheets of cells and grouped mitotic figures : these features were consistent with a melanocytic neoplasm with intermediate morphology (' BAP1 inactivated melanocytoma '; BIM) between a BAP1 inactivated melanocytic naevus and a BAP1 inactivated melanoma."
BAP1 affects BCL2
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BAP1 activates BCL2. 1 / 1
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"Independent of the neuroblastoma cell line tested, BAP1 expression promoted the downregulation of Bcl-2."
BAP1 affects BAK1
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SEMA3F binds BAK1 and BAP1. 1 / 1
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"TNK2 is known to encourage prostate tumorigenesis, xref – xref Some of the down-regulated genes, like SEMA3F , BAK1 , and BAP1 are associated with tumor suppression. xref , xref – xref Many of the down-regulated genes are also associated with decreased invasion and motility, such as DDR1 , ZYX , NES , and PLEC . xref – xref "
BAP1 affects Atrophy
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BAP1 activates Atrophy. 1 / 1
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"In pancreas, BAP1 inactivation causes organ atrophy while triggers the inactivation of tumour suppressor Hippo pathways in pancreatic KRAS mutated cancer [38, 39]."
BAP1 affects ANAPC7
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No evidence text available
BAP1 affects AMPK
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BAP1 inhibits AMPK. 1 / 1
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"BAP1 re-expression in BAP1-null UM cell lines decreased phospho-AMPK (pAMPK) and phospho-ACC (pACC) levels."
BAP1 affects AKT
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AKT binds ASXL1 and BAP1. 1 / 1
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"We found that wild-type/mutant ASXL1 and BAP1 bound to AKT in both cytoplasm and nucleus in 293T cells, suggesting that they form a complex (Fig.  xref a, xref , and Supplementary Fig.  xref )."
BAP1 affects ADSL
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BAP1 activates ADSL. 1 / 1
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"This result strongly suggests that OMVs are important for the Bap1 mediated resistance to AMPs."
BAP1 affects 6dEB
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BAP1 increases the amount of 6dEB. 1 / 1
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"It was found that an E. coli BAP1 strain harboring DEBS but no PCC complex could still produce low levels of 6dEB in the presence of propionate."
BAK1 affects SEMA3F
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SEMA3F binds BAK1 and BAP1. 1 / 1
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sparser
"TNK2 is known to encourage prostate tumorigenesis, xref – xref Some of the down-regulated genes, like SEMA3F , BAK1 , and BAP1 are associated with tumor suppression. xref , xref – xref Many of the down-regulated genes are also associated with decreased invasion and motility, such as DDR1 , ZYX , NES , and PLEC . xref – xref "
ATF3 affects DDIT3
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DDIT3 binds ATF3 and BAP1. 1 / 1
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sparser
"Mechanically, we show that BAP1 represses metabolic stress-induced UPR and cell death through activating transcription factor 3 (ATF3) and C/EBP homologous protein (CHOP), and reveal that BAP1 binds to ATF3 and CHOP promoters and inhibits their transcription."
ASXL2-AB box affects BAP1
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ASXL2-AB box activates BAP1. 1 / 1
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"Association of the ASXL2-AB box greatly stimulates BAP1 activity."
ASXL1 affects AKT
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AKT binds ASXL1 and BAP1. 1 / 1
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"We found that wild-type/mutant ASXL1 and BAP1 bound to AKT in both cytoplasm and nucleus in 293T cells, suggesting that they form a complex (Fig.  xref a, xref , and Supplementary Fig.  xref )."
ANAPC7 affects BAP1
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No evidence text available
AKT affects BAP1
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AKT binds ASXL1 and BAP1. 1 / 1
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sparser
"We found that wild-type/mutant ASXL1 and BAP1 bound to AKT in both cytoplasm and nucleus in 293T cells, suggesting that they form a complex (Fig.  xref a, xref , and Supplementary Fig.  xref )."

No evidence text available
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"Kayano et al. [40] isolated P-I metalloproteinases from B. brazili that showed activity at different concentrations and were inhibited by EDTA and DTT, but not by PMSF."
(S)-nicotine affects BAP1
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(S)-nicotine decreases the amount of BAP1. 1 / 1
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No evidence text available