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BAP1 binds LDHA. 16 / 16
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"By elucidating the interaction between BAP1 and LDHA and the subsequent effects on lactate production in melanoma cells, this work provides insights into the mechanism of BAP1-mediated metabolic regulation."
39587076
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"We then reasoned that the binding of BAP1 to LDHA was critical to mediate the translocation of LDHA to the nucleus since BAP1 but not LDHA harbored the NLS sequence."
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"By elucidating the interaction between BAP1 and LDHA and the subsequent effects on lactate production in melanoma cells, this work provides insights into the mechanism of BAP1-mediated metabolic regulation."
39587076
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"We also performed immunoprecipitation experiments in cell lines supplemented with wild-type and enzymatically inactive mutant BAP1, and interactions between LDHA and BAP1 were detected in each group (Fig. 2F)."
39587076
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"Notably, these BAP1 mutants cannot rescue the phenotype of lactate production (Fig. xref ), suggesting that full-length BAP1 was required to maintain the interaction between BAP1 and LDHA."
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"Therefore, enzyme active site mutants of BAP1 (C91G, G185R) that did not affect BAP1-LDHA interaction and BAP1 nuclear translocation were still capable of regulating the subcellular localization of LDHA."
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"To investigate whether BAP1 directly inhibits the enzymatic activity of LDHA by binding to LDHA, we generated full-length BAP1 and LDHA expression constructs, then expressed them in Escherichia coli system to obtain purified proteins by affinity chromatography (Fig. 3A)."
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"In this study, we have found that BAP1 interacts with LDHA."
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"We further performed immunoprecipitation experiments on several candidates and found that LDHA can interact with BAP1 (Fig. xref , Supplementary Fig. xref )."
39587076
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"We also performed immunoprecipitation experiments in cell lines supplemented with wild-type and enzymatically inactive mutant BAP1, and interactions between LDHA and BAP1 were detected in each group (Fig. xref )."
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"The nuclear translocation of LDHA was calculated to increase from ~15% to ~46% (Fig. xref ), indicating that the interaction between BAP1 and LDHA affected its subcellular localization."
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"The nuclear translocation of LDHA was calculated to increase from ~15% to ~46% (Fig. 4C), indicating that the interaction between BAP1 and LDHA affected its subcellular localization."
39587076
sparser
"In the present study, we found that BAP1 interacts with LDHA, which supports the nuclear translocation of LDHA."
39587076
reach
"We then reasoned that the binding of BAP1 to LDHA was critical to mediate the translocation of LDHA to the nucleus since BAP1 but not LDHA harbored the NLS sequence."
39587076
reach
"Notably, these BAP1 mutants cannot rescue the phenotype of lactate production (Fig. 5D), suggesting that full-length BAP1 was required to maintain the interaction between BAP1 and LDHA."
39587076