IndraLab

Statements

BAP1 binds COPI complex. 3 / 3
| 3
reach
"AP-immunoblotting of GFP-COPE shows interaction of COPA as expected, however BAP1 cannot be observed which is probably due to the low levels of BAP1 that bind to the COPI complex (Fig 5E)."
| PMC
reach
"Overall, the loss of mass of COPA on blot could not be tied to the loss of ubiquitin and additional experiments are required to explain this observation.To exclude that the BAP1 interaction with the COPI complex is an artifact of overexpression of the exogenous BAP1 construct, we immunoprecipitated endogenous BAP1 using different antibodies against BAP1 in cell lysate from HeLa FRT parental cells (Fig 5D)."
| PMC
reach
"It was intriguing that BAP1 has such a sequence in its C-terminus, but our mutational analysis suggests that the interaction between BAP1 and the COPI complex is not mediated through the C-terminal KxKxx sequence in BAP1."
| PMC