IndraLab

Statements

BAP1 binds MP46. 11 / 11
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sparser
"As expected, neither WZB117 nor 6-AN had any significant effect on the MP46 and MP46-BAP1 growth ( xref )."
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sparser
"We then compared FAO-oxygen consumption rate (OCR) levels between MP46 and MP46-BAP1 by Seahorse analysis."
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"First, we measured FA utilization levels between MP46 and MP46-BAP1 by incubating cells with palmitate (PAL) as the only oxidizable energy substrate."
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"We did not observe significant differences in glycolysis, glycolytic capacity or OXPHOS parameters between MP46 and MP46-BAP1 ( xref ), although SRC levels were lower in MP46 than MP46-BAP1 ( xref )."
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sparser
"Following PAL treatment, OCR levels increased significantly in MP46, but were unchanged in MP46-BAP1 ( xref ), suggesting that MP46 utilized or metabolized FA more efficiently than MP46-BAP1."
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"We also tested for FAO-dependent OCR between MP46 and MP46-BAP1 by treatment with the FAO inhibitor, perhexiline (PHX)."
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"Next, we treated MP46 and MP46-BAP1 with IACS or ETO."
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"In comparison to MP46-BAP1, the predominant (56%) metabolites increased in MP46 were related to nucleotide biosynthesis pathways, while most metabolite decreases were associated with amino acid and FA metabolism ( xref and xref )."
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"We found that MP65 (OXPHOS high ) were more resistant to both metabolic stress environments than MP65-BAP1, whereas MP46 (OXPHOS low ) were more vulnerable to stressful conditions compared to MP46-BAP1 ( xref and xref )."
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sparser
"We further investigated the metabolite levels related to FA metabolism and observed MP46 had significantly lower levels of FAs and palmitoylcarnitine, which is involved in FA transport into mitochondria for oxidation compared to MP46-BAP1, but higher acetyl-carnitine levels, a byproduct of FAO ( xref )."
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sparser
"Of note, BAP1 expression was increased in hypoxic conditions in MP46-BAP1 ( xref ), implying the association between hypoxic-induced HIF1α and exogenous BAP1 expression."
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