IndraLab

Statements

BAP1 binds GST. 5 / 5
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"Of these 30 human proteins detected, 8 proteins showed enrichment upon incubation with the nuclear protein (Fig. xref , right panel), while 22 proteins had higher levels in the GST-BAP1 fusion protein alone group, which likely represented background noise."
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"Additionally, purified GST-BAP1, but not GST control, was able to bind to His-UFL1 in vitro ( xref )."
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"To identify the domains responsible for LKB1 interaction, we constructed and purified GST-BAP1 deletion constructs."
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"Additionally, incubation with purified GST-BAP1 WT in vitro, but not the C91S mutant, caused a notable reduction in polyubiquitinated pVHL (fig."
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"We observed some differential bands above 95 kDa in the group of GST-BAP1 fusion protein incubated with PLC/PRF/5 nuclear protein, as compared with the group of GST-BAP1 fusion protein only (Fig. xref , left panel)."
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