IndraLab

Statements

BAP1 binds ASXL1 and H2AK119Ub. 4 / 4
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"The images were collected with an exposure of 0.04 seconds per frame; the total exposure time was 2 seconds for the BAP1/ASXL1-H2AK119Ub complex, for a total of 50 frames."
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"In the BAP1/ASXL1-H2AK119Ub complex, we centered a spherical mask with a 60 Å pixels radius on the BAP1/ASXL1 moiety, which resulted in a clearly resolved BAP1/ASXL1 on the nucleosome at 5."
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"Using the best-scoring particles, we ran Non-Uniform Refinement, which yielded the BAP1/ASXL1-H2AK119Ub complex at 3."
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"For the BAP1/ASXL1-H2AK119Ub complex, available X-ray crystal structures were used for initial rigid body fit into our cryo-EM maps."
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