IndraLab

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Cre activates BAP1. 3 / 3
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"As already discussed, a strong reduction in the expression of the Bap1 exons was demonstrated in the RNA-seq datasets from Bap1 Cγ1-cre relative to control GC B cells ( Supplementary Figures S3C, D ), confirming an effective Cre-mediated Bap1 gene inactivation in the cells captured for the RNA-seq."
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"At the same time, the effective Cre-mediated Bap1 to Bap1 allele conversion was demonstrated by PCR-genotyping of the genomic DNA from ex vivo stimulated Bap1 Cγ1-cre and control Bap1 Cγ1-cre B cells ( Supplementary Figures S3A, B )."
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"Again, Bap1 Cγ1-Cre B cells demonstrated normal levels of class switching over 4 days in culture ( Supplementary Figures S6A, B ), though Cre-mediated Bap1 to Bap1 allele conversion was observed as early as days 3, albeit without full loss of the Bap1 allele ( Supplementary Figures S6C, D )."
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