IndraLab

"Given that the composition and expression of PRC1 and BAP1 complexes changes extensively during development ( xref ; xref , xref ; xref ; xref ), in future work, it will be interesting to investigate how the balance between these two opposing activities is regulated at different developmental stages and how cell type-specific H2AK119ub1 levels influence the transcriptional potential of the genome."

"Recent CRISPR screening studies by Dixit’s group further identified that depletion of PRC1 subunit Ring1B (but not Ring1A) could rescue the cell death induced by loss of BAP1 xref , indicating that there is a robust and direct epigenetic dynamic occurring between the PRC1 and BAP1 complexes that require a balanced state to properly regulate gene expression and determine cell fate."

"lines used in our study identified all these PR-DUB components but failed to identify any PRC1 component as BAP1 interacting proteins, arguing against a physical interaction between BAP1 and PRC1."

"PRC1 regulates SLC7A11 expression and H2Aub occupancy on the SLC7A11 promoter. (a) ChIP–qPCR showing the binding of BMI-1 and H2Aub on the SLC7A11 promoter, and decreased binding of H2Aub on the SLC7A11 promoter upon BMI-1 knockdown. (b) ChIP– qPCR showing the binding of RNF2 and H2Aub on the SLC7A11 promoter, and decreased binding of H2Aub on the SLC7A11 promoter upon RNF-2 knockdown. (c-f) BMI-1 or RNF2 deficiency increased SLC7A11 mRNA (c, d) and protein levels (e, f) in UMRC6 cells. (g-h) BMI-1 (g) or RNF2 (h) deficiency in 786-O cells increased SLC7A11 mRNA levels. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001. lines used in our study identified all these PR-DUB components but failed to identify any PRC1 component as BAP1-interacting proteins, arguing against a physical interaction between BAP1 and PRC1."