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Statements

BAP1 binds HIF1A. 20 / 20
4 | 1 15
sparser
"We demonstrated that during hypoxia, BAP1 binds, deubiquitylates, and stabilizes HIF-1α, the master regulator of the hypoxia response and tumor cell invasion."
36656861
reach
"HIF1alpha-dependent metabolism:. Bioinformatics interrogation of The Cancer Genome Atlas (TCGA) data have associated BAP1 alterations with changes in hypoxia-inducible factor-1 alpha (HIFα) expression (25,76,77)."
33446574
sparser
"BAP1 is a key positive regulator of HIF-1α in hypoxia and binds to the N-terminal region of HIF-1α, where HIF-1α recognizes DNA and dimerizes with HIF-1β to form the heterodimeric transactivating complex HIF."
39748950
sparser
"We found that BAP1 binds to the N-terminal region of HIF-1α, where HIF-1α binds DNA and dimerizes with HIF-1β forming the heterodimeric transactivating complex HIF."
36656861
sparser
"BAP1 Interacts with HIF-1α."
36656861
sparser
"Co-immunoprecipitation (CoIP) and proximity ligation assay (PLA) experiments revealed that 1) HIF-1α and BAP1 bind to each other and co-precipitate ( xref ), and 2) the nuclei of BAP1 WT cells contained significantly more PLA positive signals—evidence of BAP1 and HIF-1α interaction—than BAP1 +/− cells ( xref )."
36656861
sparser
"We further investigated the specificity of the BAP1 interaction with HIF-1α in HEK-293 cells expressing Myc- BAP1 and HA-HIF-1α, using HA-Tag as bait."
36656861
sparser
"We established a computational model of the binding complex of BAP1 and HIF-1α."
36656861
sparser
"The NLS domain of BAP1 binds to HIF-1α-r73 and the thermodynamic stability of the binding complex increases through electrostatic interactions with DNA."
36656861
sparser
"CoIP experiments in cells co-transfected with full-length Myc-tagged BAP1 (Myc- BAP1 ) and HA-tagged full-length HIF-1α, or HIF-1α fragments covering residues 74 to 826 [HIF-1α(74-826)], 2-400 [HIF-1α(2-400)], 401-826 [HIF-1α(401-826)] ( xref ), confirmed that BAP1 binds to the N terminus region of HIF-1α [HIF-1α(2-400)] ( xref )."
36656861
sparser
"As predicted by our computational model, residues 1 to 73 of HIF-1α are essential for the interaction because HIF-1α(74-826) did not bind BAP1 ( xref )."
36656861
sparser
"All four residues forming the hydrophobic core of BAP1 must be mutated to completely abolish the binding of HIF-1α, while in the presence of single point mutations BAP1 interaction with HIF-1α is decreased but not entirely abolished ( xref )."
36656861
sparser
"A decrease in nuclear HIF-1α signal was observed in mesothelioma cases with germline BAP1 mutations, particularly those with biallelic mutations, which revealed BAP1’s interaction with HIF-1α ( xref )."
41602827
biogrid
No evidence text available
36656861
biogrid
No evidence text available
36656861
biogrid
No evidence text available
36656861
biogrid
No evidence text available
36656861
sparser
"Therefore, our data indicate that BAP1 is not required for HIF-1α-HIF-1β complex formation to functionally bind to DNA, that HIF-1β is not required for BAP1 -HIF-1α complex formation to functional binding to DNA, and that although DNA facilitates the binding of BAP1 and HIF-1α, it is not required to maintain the binding of both BAP1 -HIF-1α and BAP1 -HIF-1β."
36656861
sparser
"As recently investigated, BAP1 binds to HIF-1α [ xref ], thus regulating its activity."
40000790
sparser
"In addition, we observed that hypoxic conditions increase exogenous BAP1 expression, raising the possibility of future studies to investigate HIF1α-BAP1 cross-talk, and its role in metabolic alterations."
33208912