IndraLab

Statements

BAP1 binds Flag. 12 / 12
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"In support of the notion that BAP1 might be an upstream DUB for MYCN, Flag-BAP1 and HA-MYCN were co-transfected into 293T cells and the immunoprecipitation (IP) assay was performed to examine whether BAP1 will interact with MYCN."
37543638
sparser
"Immunofluorescence (IF) staining of HEK293T cells co-transfected with Flag-BAP1 and Myc-SUFU revealed strong cytoplasmic co-localization of the two proteins, with minimal nuclear overlap (Figs.  xref B and S1A), further supporting their protein-protein interaction in cells."
41484776
sparser
"HeLa cells stably expressing Flag-BAP1 were selected and lysed with IP150 buffer (200 mM Tris-HCl, pH 7.9, 15% glycerol, 1 mM EDTA, 1 mM DTT, 0.2 mM PMSF, 0.5% NP-40, and 150 mM KCl)."
28802580
sparser
"To identify the O -GlcNAcylated region of BAP1, Flag-BAP1 deletion fragments (N-terminal, amino acids [aa] 1–239; C-terminal, aa 240–729) were generated and transfected into HCT116 cells."
28802580
sparser
"Whole-cell lysates from HEK293 cells transfected with Flag-BAP1 were pulled down using WGA agarose beads."
28802580
sparser
"To investigate the biochemical function of BAP1, we performed affinity purification of the BAP1-associated protein complex using HeLa cells stably expressing Flag-BAP1."
28802580
sparser
"Moreover, we observed that Flag-ASXL1 interacted with endogenous HCF-1, FOXK1 and FOXK2 in BAP1 knockdown HEK293T cells (Fig.  xref G), and that Flag-BAP1 interacted with endogenous HCF-1, FOXK1, and FOXK2 in ASXL1 knockdown HEK293T cells (Fig.  xref H)."
32683582
sparser
"Both Flag-BAP1 and GlcNAc were detected in the elutes of WGA beads but not in control agarose (A/G) beads, further supporting that BAP1 is modified by O- GlcNAc ( Fig. 2 B)."
28802580
reach
"Full-length BAP1 (1-729), the BAP1 N terminus (1-240) and the BAP1 C terminus (597-729) interacted with KLF5-3 x Flag (XREF_FIG)."
26419610
sparser
"To directly check whether MYCN protein stability is regulated by BAP1-dependent deubiquitination, the Flag-BAP1, His-Ub and HA-MYCN were co-transfected into 293T cells."
37543638
sparser
"Transfection of increasing doses of Flag-BAP1 (0, 2 and 4 µg) in HEK293T cells resulted in a dose-dependent elevation of SUFU protein levels (Fig.  xref C), without altering SUFU mRNA expression (Fig.  xref D), indicating a post-transcriptional mechanism."
41484776
sparser
"Cell lines with stable expression of Flag-BAP1 WT or mutant were generated as described in our previous publication [18]."
30907299