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ASXL1 activates BAP1. 40 / 40
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"ASXL1 activates BAP1 [ xref ]."
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"Increasing concentrations of TG2-179-1 were incubated with H2A-Ub nucleosomes containing the DEUBAD domain of ASXL1 (ASXL1 DEU ), which activates BAP1 by increasing its affinity for the ubiquitin moiety of H2A-Ub xref ."
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"Because of the strong conservation of key elements between UCH-L5 and RPN13 DEU and BAP1 and ASXL1, we anticipate that the ASXL1 DEUBAD domain employs similar strategies to activate BAP1."
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"Increasing concentrations of TG2-179-1 were incubated with H2A-Ub nucleosomes containing the DEUBAD domain of ASXL1 (ASXL1 ), which activates BAP1 by increasing its affinity for the ubiquitin moiety of H2A-Ub ."
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"As part of the Polycomb repression machinery, BAP1 is activated by the deubiquitinase adaptor domain of ASXL1 mediating gene repression by cleaving ubiquitin (Ub) from histone H2A in nucleosomes."
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"The molecular mechanism of BAP1 activation by ASXL1 remains elusive, as no structures are available for either BAP1 or ASXL1."
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"A recent study suggests that ASXL1 might activate Bap1 in a similar manner xref ."
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"Several studies reported that ASXL1 truncation enhanced the catalytic deubiquitinating activity of BAP1 towards mono-ubiquitinated H2AK119 [ 20 , 36 ]."
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"The molecular mechanism of BAP1 activation by ASXL1 remains elusive, as no structures are available for either BAP1 or ASXL1."
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"A previous report showed that the deubiquitinase adapter (DEUBAD) domain of ASXL1 increases BAP1 's affinity for ubiquitin on H2A 15, and we showed that monoubiquitinated ASXL1-MT enhances nuclear retention of BAP1."
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"BAP1 is activated by ASXL1 in a similar manner where the DEUBAD domain of ASXL1 increases affinity of BAP1 for the ubiquitinated substrate [98]."
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"To further confirm that GGNBP2 loss could affect the activation of BAP1 by ASXL1 in deubiquitinating H2A, Ggnbp2KO cells were transfected with ASXL1 siRNA together with Ggnbp2 overexpression plasmids."
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"ASXL1 Modulates BAP1 DUB Activity and RAR Repression In (A) Appearance of Asxl1 null embryos derived from E18.0."
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"For instance, ASXL1 truncations that lead to a loss of the C-terminal PHD domain (Fig.  xref , [1]) can globally erase H2AK119Ub xref , potentially because these ASXL1 molecules can moderately activate BAP1 with their Deubad domains but fail to locally concentrate the PR-DUB in particular areas of the genome."
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"BAP1 is activated by ASXL1 and deubiquitylates mono-ubiquitylation of H2AK119 ( Daou et al ., 2015 ; Sahtoe et al ., 2016 ) ."
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"Like UCH37, BAP1 associates with a partner protein, ASXL1, which activates BAP1 and is required for the BAP1 mediated deubiquitylation of H2A in nucleosomes."
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"More specifically, the PR-DUB complex, composed of BAP1 activated by ASXL1 [103], is recruited to DNA and forms a DNA-protein complex which regulates genes responsible for hematopoietic development, including the HOX gene family [104] (Fig. 1C)."
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"Moreover, as a component of the BAP1 histone H2A deubiquitinase complex, ASXL1 truncation increases its stability, strengthening the association of the BAP1 complex with chromatin, driving oncogenic gene expression [28]."
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"BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression, but the mechanism of this reaction remains poorly defined."
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"The DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing its affinity for ubiquitin."
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"Like on Ub-AMC, ASXL1 1-390 could activate BAP1 to the same extent as ASXL1 DEU (XREF_FIG), but on the nucleosomal substrates the activation observed was much more significant than on the minimal substrate."
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"ASXL1 DEU stimulates BAP1 by lowering the K M on Ub-AMC (XREF_SUPPLEMENTARY)."
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"A recent study suggests that ASXL1 might activate Bap1 in a similar manner 92."
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"Therefore we tested whether ASXL1 DEU can activate BAP1 by increasing its affinity for ubiquitin."
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"While the conjugate inhibited BAP1 with an IC 50 of ~ 45muM, the IC 50 of the BAP1 and ASXL1 DEU complex was> 10-fold lower indicating that ASXL1 DEU induces a higher affinity of BAP1 to the conjugate (XREF_FIG and XREF_SUPPLEMENTARY)."
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"In summary, we have shown that ASXL1 DEU activates BAP1 by increasing the affinity for ubiquitin through a combination of mild effects including stabilization of the BAP1 ULD anchor."
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"In this paper we describe that similar to the homologous UCH-L5 and RPN13 DEU complex, the DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing BAP1 's affinity specifically for the ubiquitin in the substrate, through a combination of mild effects."
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"The N-terminal region of ASXL1, specifically amino acids 2-365 of ASXL1, is sufficient to promote the H2AK119Ub DUB activity of BAP1 on nucleosomal templates in vitro XREF_BIBR."
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"Moreover, both ASXL1 (1-1305) and ASXL1 (1-479) stabilized the expression of BAP1 protein (XREF_SUPPLEMENTARY) and all truncated ASXL1 proteins enhanced the H2AK119Ub-DUB activity of BAP1 in HEK293T cells to a substantially greater extent than observed for full-length ASXL1 (XREF_FIG)."
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"Studies have revealed that BAP1 is activated by ASXL1 and can selectively deubiquitinate the H2Aub K119 site, which is involved in regulating cellular physiological activities and various cell damage modes [ xref ]."
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"BAP1 is activated by ASXL1 and deubiquitylates mono-ubiquitylation of H2AK119 ( xref ; xref )."
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"6 In line with these findings, several reports have indicated that truncated ASXL1 enhances BAP1 complex activity, thereby promoting depletion of the H2AK119Ub mark and aberrant myeloid differentiation."
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"For instance, ASXL1 truncations that lead to a loss of the C-terminal PHD domain can globally erase H2AK119Ub 39, potentially because these ASXL1 molecules can moderately activate BAP1 with their Deubad domains but fail to locally concentrate the PR-DUB in particular areas of the genome."
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"To further confirm that GGNBP2 loss could affect the activation of BAP1 by ASXL1 in deubiquitinating H2A, Ggnbp2KO cells were transfected with ASXL1 siRNA together with Ggnbp2 overexpression plasmids."
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"ASXL1 also recruits BAP1 to the RA responsive promoter and promotes BAP1 activity therein."
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"Inactivation of Mettl4 generated a ∼5-fold increase in the number of called Asxl1 peaks (Figures 5D and 5E; Table S2) and a 41.3% increase in Bap1 peaks (Figure 5D) identified using stringent cutoff criteria (FDR adjusted p < 0.05), in agreement with Asxl1-mediated stabilization of Bap1."
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"BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression, but the mechanism of this reaction remains poorly defined."
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"The tumor suppressor BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression."
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"The ULD is conserved in UCH family member BAP1 that is activated by the ASXL1 DEUBAD domain to deubiquitinate H2A."
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"The PRC2 component, ASXL1, activates BAP-1 by increasing its affinity for ubiquitin."
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