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ASXL1 activates BAP1. 29 / 29
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"In this paper we describe that similar to the homologous UCH-L5 and RPN13 DEU complex, the DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing BAP1 's affinity specifically for the ubiquitin in the substrate, through a combination of mild effects."

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"Like UCH37, BAP1 associates with a partner protein, ASXL1, which activates BAP1 and is required for the BAP1 mediated deubiquitylation of H2A in nucleosomes."

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"As part of the Polycomb repression machinery, BAP1 is activated by the deubiquitinase adaptor domain of ASXL1 mediating gene repression by cleaving ubiquitin (Ub) from histone H2A in nucleosomes."

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"BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression, but the mechanism of this reaction remains poorly defined."

sparser
"A recent study suggests that ASXL1 might activate Bap1 in a similar manner xref ."

sparser
"BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression, but the mechanism of this reaction remains poorly defined."

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"ASXL1 DEU stimulates BAP1 by lowering the K M on Ub-AMC (XREF_SUPPLEMENTARY)."

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"A previous report showed that the deubiquitinase adapter (DEUBAD) domain of ASXL1 increases BAP1 's affinity for ubiquitin on H2A 15, and we showed that monoubiquitinated ASXL1-MT enhances nuclear retention of BAP1."

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"BAP1 is activated by ASXL1 in a similar manner where the DEUBAD domain of ASXL1 increases affinity of BAP1 for the ubiquitinated substrate [98]."

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"In summary, we have shown that ASXL1 DEU activates BAP1 by increasing the affinity for ubiquitin through a combination of mild effects including stabilization of the BAP1 ULD anchor."

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"The DEUBAD domains of ASXL1, ASXL2 and ASXL3 can activate BAP1 by increasing its affinity for ubiquitin."

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"The molecular mechanism of BAP1 activation by ASXL1 remains elusive, as no structures are available for either BAP1 or ASXL1."

sparser
"The molecular mechanism of BAP1 activation by ASXL1 remains elusive, as no structures are available for either BAP1 or ASXL1."

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"For instance, ASXL1 truncations that lead to a loss of the C-terminal PHD domain can globally erase H2AK119Ub 39, potentially because these ASXL1 molecules can moderately activate BAP1 with their Deubad domains but fail to locally concentrate the PR-DUB in particular areas of the genome."

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"Therefore we tested whether ASXL1 DEU can activate BAP1 by increasing its affinity for ubiquitin."

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"The tumor suppressor BAP1 is activated by ASXL1 to deubiquitinate mono-ubiquitinated H2A at K119 in Polycomb gene repression."

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"The ULD is conserved in UCH family member BAP1 that is activated by the ASXL1 DEUBAD domain to deubiquitinate H2A."

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"Like on Ub-AMC, ASXL1 1-390 could activate BAP1 to the same extent as ASXL1 DEU (XREF_FIG), but on the nucleosomal substrates the activation observed was much more significant than on the minimal substrate."

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"Because of the strong conservation of key elements between UCH-L5 and RPN13 DEU and BAP1 and ASXL1, we anticipate that the ASXL1 DEUBAD domain employs similar strategies to activate BAP1."

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"BAP1 is activated by ASXL1 and deubiquitylates mono-ubiquitylation of H2AK119 ( xref ; xref )."

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"ASXL1 Modulates BAP1 DUB Activity and RAR Repression In (A) Appearance of Asxl1 null embryos derived from E18.0."

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"While the conjugate inhibited BAP1 with an IC 50 of ~ 45muM, the IC 50 of the BAP1 and ASXL1 DEU complex was> 10-fold lower indicating that ASXL1 DEU induces a higher affinity of BAP1 to the conjugate (XREF_FIG and XREF_SUPPLEMENTARY)."

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"6 In line with these findings, several reports have indicated that truncated ASXL1 enhances BAP1 complex activity, thereby promoting depletion of the H2AK119Ub mark and aberrant myeloid differentiation."

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"For instance, ASXL1 truncations that lead to a loss of the C-terminal PHD domain (Fig.  xref , [1]) can globally erase H2AK119Ub xref , potentially because these ASXL1 molecules can moderately activate BAP1 with their Deubad domains but fail to locally concentrate the PR-DUB in particular areas of the genome."

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"A recent study suggests that ASXL1 might activate Bap1 in a similar manner 92."

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"The N-terminal region of ASXL1, specifically amino acids 2-365 of ASXL1, is sufficient to promote the H2AK119Ub DUB activity of BAP1 on nucleosomal templates in vitro XREF_BIBR."

eidos
"BAP1 is activated by ASXL1 and deubiquitylates mono-ubiquitylation of H2AK119 ( Daou et al ., 2015 ; Sahtoe et al ., 2016 ) ."

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"ASXL1 also recruits BAP1 to the RA responsive promoter and promotes BAP1 activity therein."

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"Moreover, both ASXL1 (1-1305) and ASXL1 (1-479) stabilized the expression of BAP1 protein (XREF_SUPPLEMENTARY) and all truncated ASXL1 proteins enhanced the H2AK119Ub-DUB activity of BAP1 in HEK293T cells to a substantially greater extent than observed for full-length ASXL1 (XREF_FIG)."