IndraLab

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BAP1 binds VPS. 12 / 12
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"For example, binding between LecB and CdrA to the Psl polysaccharide in P. aeruginosa biofilms contribute to cell–cell junction formation, and binding of RbmA, RbmC, and Bap1 to VPS contribute to biofilm formation in Vibrio cholerae."
37693546
reach
"These observations indicate that Bap1 and RbmC could bind VPS, and these interactions may influence the spatial localization of these matrix proteins."
26104709
sparser
"Importantly, vesicles prepared from the strain unable to produce RbmA/RbmC/Bap1/VPS still affected microcolony formation within the biofilm although not in the same way as OMVs isolated from the parent strain."
38206049
sparser
"These observations indicate that Bap1 and RbmC could bind VPS, and these interactions may influence the spatial localization of these matrix proteins."
26104709
sparser
"Next, we investigated how the RbmC and Bap1 matrix proteins interact with VPS during biofilm formation."
22798614
sparser
"We combine genetics, microscopy, simulations, and biochemical analyses to show that V. cholerae cells are not attracted to the main matrix component ( Vibrio polysaccharide, VPS), but can be attached to each other and to the VPS network through surface-associated VPS and crosslinks formed by the protein Bap1."
39747177
sparser
"When we added Bap1 to a growing Δ ABC culture, we found disordered cell aggregates loosely connected by the VPS-Bap1 network (Fig.  xref and Fig.  xref )."
39747177
sparser
"Crucially, the recognition of community membership is closely tied to the active secretion of VPS: cells coated by VPS – which only occurs when the exopolysaccharide is actively being produced – are recognized by the existing VPS-Bap1 network or other VPS-coated cells and consequently included within the biofilm through bridging-aggregation."
39747177
sparser
"Because the matrix proteins RbmA, RbmC, and Bap1 all directly interact with VPS, biofilms lacking VPS are thought not to retain the three primary matrix proteins, either."
41279305
sparser
"Interestingly, two adhesins — Bap1 ( B iofilm- a ssociated p rotein 1) and its homologue RbmC ( R ugosity and b iofilm m odulator C) directly bind to VPS [ xref ], thus resulting in a cross-linking of cells with the matrix similar to CdrA in P. aeruginosa ."
38358118
sparser
"Interestingly, we did not detect fragments larger than an octasaccharide and we did not observe ladders in our native gels ( xref ), which would be expected if there were appreciable levels of VPS fragments that could bind multiple Bap1 molecules at the same time."
39621768
sparser
"This may also explain why we do not see intermediately-sized native gel bands during RbmB cleavage arising from VPS fragments bound to multiple Bap1 molecules ( xref )."
39621768