IndraLab

Statements

FlaG binds BAP1. 12 / 12
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"However, FLAG-BAP1 failed to attenuate a decrease in the level of HA-KEAP1ΔBTB induced by treatment of CHX in 293T-HA-KEAP1ΔBTB cells ( xref C, right panel), suggesting that the BTB domain is responsible for maintaining the protein stability of KEAP1 by BAP1."
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"Our results show that transduced FLAG-BAP1 is mostly located in the cytosol ( xref A)."
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"Similar transverse distributions of each protein were observed in biofilms formed by a Δbap1 mutant expressing Bap1-FLAG from a plasmid alone and by a ΔrbmA mutant expressing RbmA-FLAG from a plasmid alone (data not shown)."
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"In addition, lentiviral transduction of FLAG-BAP1 suppressed the migration ( xref E) and the invasion ( xref F) of A549 cells, and potentiated cisplatin-induced apoptosis in A549 cells ( xref G,H)."
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"Real-time RT-PCR analysis demonstrates that lentiviral transduction of FLAG-BAP1 significantly decreased the mRNA level of NRF2 target genes such as heme oxygenase-1 (Hmox1), NADPH:quinone oxidoreductase-1 (Nqo1), glutamate cysteine ligase catalytic subunit (Gclc), and aldo-keto reductase family 1 member B10 (Akr1b10) in A549 cells ( xref D)."
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"We then conducted ChIP-qPCR assays in UMRC-6 cells reconstituted with FLAG-BAP1 or empty vector."
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"Immunoprecipitation revealed that transiently overexpressed FLAG-BAP1 bound to both transiently overexpressed GFP-ERK1 and GFP-ERK2 ( xref )."
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"We observed that FLAG-BAP1 ( xref B, upper panel), but not FLAG-UCHL3 ( xref B, lower panel), directly binds to HA-KEAP1."
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"However, FLAG-BAP1 did not bind to HA-NRF2 ( xref C)."
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"Our result shows that FLAG-BAP1 does not selectively bind to HA-KEAP1ΔBTB ( xref A, right panel), suggesting that BAP1 binds to the BTB domain of KEAP1."
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"In order to identify the domains of BAP1 that interact with KEAP1, we generated FLAG-BAP1 mutant plasmids lacking individual domains (FLAG-BAP1ΔCD, FLAG-BAP1ΔCC1, FLAG-BAP1ΔCL, FLAG-BAP1ΔCC2, FLAG-BAP1ΔULD, and FLAG-BAP1ΔCTE)."
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"Our results show that treatment of CHX decreased the level of HA-KEAP1, and this event was significantly attenuated by overexpression of FLAG-BAP1 in 293T-HA-KEAP1 cells ( xref C, left panel)."
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