IndraLab
Statements
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
USP1 is modified
63
|
53
24
USP1 is phosphorylated.
63
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41
24
rlimsp
"Another reversible modification of USP1 is phosphorylation at Ser313 by cyclin-dependent kinases (CDKs) [84]. Two functional consequences of Ser313 phosphorylation have been reported. On one hand, phosphorylation of this residue, which is located within USP1 degron motif (Figure 2), may contribute to regulate the cell-cycle dependent levels of USP1, by preventing its degradation in mitosis [84]."
sparser
"Our finding that phosphorylation of USP1 at S313 is dispensable for UAF1 binding in cells, led us to use the relocation assay to directly compare the relative contribution of two proposed UAF1-binding sites in USP1: the 235–408 fragment containing the S313 residue [ xref ], and the 420–520 fragment [ xref ]."
USP1 is ubiquitinated.
|
7
USP1 is dephosphorylated.
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3
USP1 is acetylated.
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2
reach
"The uncoupling of monoubiquitination and chromatin enrichment from nuclear foci formation and effective ICL repair has previously been observed : Usp1 -/- murine embryonic fibroblasts display elevated levels of monoubiquitinated Fancd2 and Fancd2 chromatin localization, but fail to support Fancd2 nuclear foci formation and exhibit ICL-hypersensitivity XREF_BIBR."
sparser
"Researchers verified that the expression of some circular isoforms, such as the circRNAs derived from the DCC (deleted in colorectal cancer) gene, diversified across a range of human tissues and was not correlated with its cognate linear mRNA expression. xref Because of the up‐regulation of circ‐USP1 in GECs, we are interested to explore whether the dysregulation of circ‐USP1 was associated with the regulation of BTB function."
reach
"Evidence shows that depletion of USP1 in either murine models or chicken DT40 cells enhances chromatin loading of the ID complex in the absence of exogenous DNA damage, but only to similar levels as observed following mitomycin C (MMC) treatment, suggesting the existence of alternative mechanisms to restrain ID complex loading at DNA lesions."
reach
"Williams et al. also recently reported that USP1 and UAF1 deubiquitinates and prevents proteasomal degradation of ID (inhibitor of DNA binding) proteins, 13 which have been shown to activate multiple pathways involved in tumor progression, including preservation of the cancer stem cell phenotype."
sparser
"Importantly, the results revealed that neither mutant complex is able to catalyze FANCD2 deubiquitination (Fig. xref , lanes 5 and 7) even though, as documented above and in Supplementary Fig. xref , both UAF1 mutants retain all other known biochemical attributes, namely, interactions with RAD51AP1, USP1, and FANCI, and also the ability to enhance the DUB activity of USP1."
reach
"Furthermore, we found that USP1 overexpression decreased the polyubiquitination levels in WT, K6R, K11R, K27R, K29R, K33R, and K63R HA-ubiquitin mutants but not in K48R mutant, suggesting USP1 promotes KDM4A K48 linked deubiquitin; however, which E3 ubiquitin ligase mediated KDM4A ubiquitin and degradation is still unclear."
USP1 affects cell population proliferation
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4
19
USP1 activates cell population proliferation.
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4
13
USP1 activates cell population proliferation. 10 / 18
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4
13
reach
"The numerous data demonstrate that inhibition of USP1 suppresses the proliferation and viability of malignant cells, sensitizes them to radiation and increases their sensitivity to various chemo- therapeutic agents, which opens up new opportunities for combined therapy of malignant neoplasms."
reach
"The supportive evidence is as follows : (i) USP1 knockdown decreased PC cell proliferation in vitro and tumorigenesis in vivo, and promoted the response to therapeutic agent enzalutamide; and (ii) inhibition of USP1 sensitized cancer cells to therapeutic agent enzalutamide, whereas this effect was blunted by overexpression of KDM4A."
USP1 inhibits cell population proliferation.
|
6
USP1 affects cell differentiation
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23
USP1 inhibits cell differentiation.
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17
USP1 inhibits cell differentiation. 10 / 18
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17
reach
"Notably, ML323, a specific inhibitor of the USP1/UAF1 deubiquitinase complex, inhibited Th17-cell differentiation and promoted Treg-cell differentiation in vitro and in vivo, indicating that ML323 might be a promising candidate for the treatment of diseases associated with an imbalance between Th17 and Treg cells."
USP1 activates cell differentiation.
|
6
USP1 activates cell differentiation. 6 / 6
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6
reach
"Notably, ML323, a specific inhibitor of the USP1/UAF1 deubiquitinase complex, inhibited Th17-cell differentiation and promoted Treg-cell differentiation in vitro and in vivo, indicating that ML323 might be a promising candidate for the treatment of diseases associated with an imbalance between Th17 and Treg cells."
reach
"Mechanistically, we found that PDGF signaling regulated the expression of the E2F transcription factors, which directly bound to and activated Usp1 Furthermore, PDGF mediated expression of USP1 led to the stabilization of Inhibitor of DNA binding 2 (ID2), which we found to be required for glioma cell survival."
USP1 affects cellular senescence
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17
USP1 activates cellular senescence.
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12
USP1 inhibits cellular senescence.
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5
USP1 affects apoptotic process
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5
11
USP1 inhibits apoptotic process.
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5
5
USP1 inhibits apoptotic process. 10 / 10
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5
5
eidos
"Inhibition of USP1 induces apoptosis via ID1 / AKT pathway in B-cell acute lymphoblastic leukemia cells Deubiquitylating enzyme ubiquitin-specific protease 1 ( USP1 ) has been reported to be aberrantly overexpressed in cancers , and it plays a critical role in regulating various cellular processes , such as cell proliferation , apoptosis , and cell differentiation ."
USP1 activates apoptotic process.
|
6
reach
"The specificity of SJB was confirmed by various experiments : first, we show that SJB potently and selectively block USP1 activity without inhibiting other DUBs (USP2/USP5/USP7/USP14/UCH37); second, SJB inhibited binding of USP1 with HA-Ub-VS probe, but it did not affect labeling of other DUBs with probe; and third, SJB inhibited USP1, but not USP2 or USP7, triggered cleavage of ubiquitin tetramer chains."
ML323 affects USP1
|
3
12
sparser
"Nevertheless, assessment of the temporal pattern of these interactions in response to MMS treatment and in comparison with the timeline of PCNA ubiquitination showed an increased interaction between TonEBP and SHPRH at 1 h after MMS treatment, concomitantly with a decreased interaction between TonEBP and USP1 ( xref D)."
sparser
"Importantly, the results revealed that neither mutant complex is able to catalyze FANCD2 deubiquitination (Fig. xref , lanes 5 and 7) even though, as documented above and in Supplementary Fig. xref , both UAF1 mutants retain all other known biochemical attributes, namely, interactions with RAD51AP1, USP1, and FANCI, and also the ability to enhance the DUB activity of USP1."
sparser
"Importantly, the results revealed that neither mutant complex is able to catalyze FANCD2 deubiquitination (Fig. xref , lanes 5 and 7) even though, as documented above and in Supplementary Fig. xref , both UAF1 mutants retain all other known biochemical attributes, namely, interactions with RAD51AP1, USP1, and FANCI, and also the ability to enhance the DUB activity of USP1."
sparser
"Usp1-LacZ reporter mice were infected with lymphocytic choriomeningitis virus (LCMV) and peripheral blood CD8 + T cells specific for the immunodominant epitope GP 33 ( xref , xref ) or the subdominant epitope GP 276 ( xref , xref ) were assessed for Usp1 reporter activity over the course of infection."
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
sparser
"Usp1-LacZ reporter mice were infected with lymphocytic choriomeningitis virus (LCMV) and peripheral blood CD8 + T cells specific for the immunodominant epitope GP 33 ( xref , xref ) or the subdominant epitope GP 276 ( xref , xref ) were assessed for Usp1 reporter activity over the course of infection."
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
USP1 decreases the amount of USP1.
|
3
reach
"The important regulatory role of USP1 in DNA repair, supported by the finding that USP1 gene knockout in model systems leads to DNA damage hypersensitivity [XREF_BIBR - XREF_BIBR], together with the observation that USP1 is frequently overexpressed in tumors, suggests that USP1 could be a relevant target for cancer therapy, whose inhibition might contribute to overcome chemoresistance."
USP1 activates USP1.
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3
USP1 ubiquitinates USP1.
|
1
reach
"However, it is clear that multiple conserved residues (N21, R22, L23, S24, and K26) that are within a short N-terminal region may play a role in driving USP1-mediated FANCD2-Ub deubiquitination.Mutation or deletion of the USP1 N-terminus specifically and negatively impacts FANCD2-Ub deubiquitination."
USP1 affects Neoplasm Metastasis
|
9
USP1 affects EcR-B1
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9
USP1 binds EcR-B1.
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7
USP1 inhibits EcR-B1.
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2
SJB affects USP1
|
9
USP1 affects hsFANCD2-Ub
|
8
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
USP1 affects PC
|
8
USP1 inhibits PC.
|
5
USP1 activates PC.
|
3
reach
"The supportive evidence is as follows : (i) USP1 knockdown decreased PC cell proliferation in vitro and tumorigenesis in vivo, and promoted the response to therapeutic agent enzalutamide; and (ii) inhibition of USP1 sensitized cancer cells to therapeutic agent enzalutamide, whereas this effect was blunted by overexpression of KDM4A."
USP1 affects DNA repair
|
1
7
USP1 activates DNA repair.
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1
5
USP1 activates DNA repair. 6 / 6
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1
5
reach
"Because CHK1 is a cell cycle regulated and DNA damage checkpoint protein, we asked whether (a) the downregulation of CHK1 in USP1 depleted cells is an experimental artefact resulting from modifications of the cell cycle and/or altered DNA repair capabilities caused by USP1 depletion and (b) USP1 depletion modifies the G2 checkpoint in response to DNA damage."
USP1 inhibits DNA repair.
|
2
USP1 inhibits DNA repair. 2 / 2
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2
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
sparser
"In the landmark mechanism, a ParA protein serves as an adaptor between a landmark protein and a protein to be localized as described for MinD interacting with MinJ in B. subtilis , ParA interacting with TipN in C. crescentus , and ParA1, FlhG, and ParC interacting with HubP in V. cholerae ."
EcR-B1 affects USP1
|
7
Phenethyl isothiocyanate affects USP1
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4
2
Phenethyl isothiocyanate inhibits USP1.
|
2
2
Phenethyl isothiocyanate decreases the amount of USP1.
|
2
Methoprene affects USP1
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5
Methoprene increases the amount of USP1.
|
3
Methoprene activates USP1.
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2
Methoprene activates USP1. 2 / 2
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2
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
USP1 affects Neoplasm Invasiveness
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5
USP1 activates Neoplasm Invasiveness.
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4
USP1 inhibits Neoplasm Invasiveness.
|
1
USP1 inhibits Neoplasm Invasiveness. 1 / 1
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1
USP1 affects Genomic Instability
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5
USP1 inhibits Genomic Instability.
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3
USP1 activates Genomic Instability.
|
2
USP1 activates Genomic Instability. 2 / 2
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2
reach
"Interestingly, siRNA mediated knock-down of FAN1 and USP1, the enzyme responsible for deubiquitylating PCNA, caused similar genomic instability in U2OS cells, but the number of DSBs in the double knock-down was increased approximately two-fold, thus indicating that an increase in ub-PCNA may worsen the genomic instability in FAN1 depleted cells."
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5
|
3
USP1 activates DNA damage response, detection of DNA damage.
|
2
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
reach
"We conclude that the E1, UAF1, and USP1 complex supports the processing of late bidirectional theta RIs and propose that after the separation of bidirectional theta daughter genomes, the formerly converged replication forks undergo BLM mediated restart, thus initiating the unidirectional theta replication of HPV genomes."
reach
"The importance of the E1, UAF1, and USP1 complex for efficient HPV replication has been previously reported; however, we demonstrated that the lower replication efficiency of mutant HPV11 genomes encoding a E1 protein defective for UAF1 binding is associated with decreased production of RIs generated by the unidirectional mechanism, while bidirectional theta replication was affected to a lesser degree (XREF_FIG)."
APC/C Cdh1 affects USP1
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5
USP1 affects localization
|
4
USP1 affects hsFANCI-Ub
|
4
USP1 affects homologous recombination
|
4
USP1 bound to WDR48 activates homologous recombination. 2 / 2
|
2
reach
"Additionally, the same study disrupted the NHEJ pathway, via knockout of Ku70 (also known as XRCC6 or X-Ray repair cross complementing 6), in UAF1 -/-/- cells which restored the cellular resistance to camptothecin and the PARP inhibitor suggesting that the USP1 and UAF1 complex promotes HR, at least in part by suppressing NHEJ."
USP1 activates homologous recombination. 2 / 2
|
2
USP1 affects growth
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4
reach
"To our interest, USP1 reportedly destroys K63 linked polyubiquitin chains on histones and then promotes to recruit 53BP1 at DNA damage sites; another previous report found that KDM4A regulates DNA repair by controlling the recruitment of 53BP1 at DNA damage sites and RNF8 dependent degradation of KDM4A regulates DNA repair by controlling the recruitment of 53BP1 at DNA damage sites."
USP1 affects Stomach Neoplasms
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4
reach
"Stalled forks are protected by a plethora of repair and replication factors, including RIF1, which inhibits end resection, the MRN-interacting protein MRNIP, the TLS suppressor USP1 which regulates PCNA via de-ubiquitination, HR proteins (RAD51, BRCA1, BRCA2, FANCD2), and RADX which regulates RAD51 [30,68–73]."
sparser
"In the landmark mechanism, a ParA protein serves as an adaptor between a landmark protein and a protein to be localized as described for MinD interacting with MinJ in B. subtilis , ParA interacting with TipN in C. crescentus , and ParA1, FlhG, and ParC interacting with HubP in V. cholerae ."
UAF1 affects USP1
|
4
sparser
"In the landmark mechanism, a ParA protein serves as an adaptor between a landmark protein and a protein to be localized as described for MinD interacting with MinJ in B. subtilis , ParA interacting with TipN in C. crescentus , and ParA1, FlhG, and ParC interacting with HubP in V. cholerae ."
Magnetite nanoparticle affects USP1
3
|
Magnetite nanoparticle decreases the amount of USP1.
2
|
Magnetite nanoparticle increases the amount of USP1.
1
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USP1 affects hsPCNA-Ub
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3
USP1 affects cell growth
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2
1
USP1 affects cell cycle
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1
2
USP1 activates cell cycle.
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1
1
USP1 inhibits cell cycle.
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1
USP1 inhibits cell cycle. 1 / 1
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1
USP1 affects UAF
|
3
reach
"Additional structural information modeling interaction sites for ML323 (as well other USP1 inhibitors) will help define which class of DUB inhibitors this drug falls into and will be instrumental moving forward to improve strategies for selectively targeting the UAF-USP1 complex and potentially other DUBs therapeutically.Key resources and reagents."
USP1 affects TAp63
|
3
USP1 affects GC metastasis
|
3
UAF affects USP1
|
3
reach
"Additional structural information modeling interaction sites for ML323 (as well other USP1 inhibitors) will help define which class of DUB inhibitors this drug falls into and will be instrumental moving forward to improve strategies for selectively targeting the UAF-USP1 complex and potentially other DUBs therapeutically.Key resources and reagents."
SJB3-019A affects USP1
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1
2
Proteasome affects USP1
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3
Parkinson Disease affects USP1
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3
ND-EZH2 affects USP1
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3
APC Cdh1 affects USP1
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3
17alpha-ethynylestradiol affects USP1
3
|
RDUB affects USP1
|
2
Pirinixic acid affects USP1
2
|
MiR-192-5p affects USP1
|
2
Hsa-miR-7703 affects USP1
2
|
Hsa-miR-766-3p affects USP1
2
|
Hsa-miR-6776-5p affects USP1
2
|
Hsa-miR-6742-3p affects USP1
2
|
Hsa-miR-6720-5p affects USP1
2
|
Hsa-miR-661 affects USP1
2
|
Hsa-miR-6512-3p affects USP1
2
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Hsa-miR-625-3p affects USP1
2
|
Hsa-miR-5683 affects USP1
2
|
Hsa-miR-542-3p affects USP1
2
|
Hsa-miR-517c-3p affects USP1
2
|
Hsa-miR-517b-3p affects USP1
2
|
Hsa-miR-517a-3p affects USP1
2
|
Hsa-miR-508-5p affects USP1
2
|
Hsa-miR-4793-3p affects USP1
2
|
Hsa-miR-4536-3p affects USP1
2
|
Hsa-miR-4517 affects USP1
2
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Hsa-miR-4424 affects USP1
2
|
Hsa-miR-375 affects USP1
2
|
Hsa-miR-298 affects USP1
2
|
Hsa-miR-1185-2-3p affects USP1
2
|
Hsa-miR-1185-1-3p affects USP1
2
|
Hsa-let-7f-2-3p affects USP1
2
|
Doxycycline affects USP1
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2
Doxycycline inhibits USP1.
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1
Doxycycline inhibits USP1. 1 / 1
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1
Doxycycline decreases the amount of USP1.
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1
Doxycycline decreases the amount of USP1. 1 / 1
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1
Dibutyl phthalate affects USP1
2
|
Cyclosporin A affects USP1
2
|
Bisphenol A affects USP1
2
|
Bisphenol A demethylates USP1.
1
|
Bisphenol A decreases the amount of USP1.
1
|
Benzo[a]pyrene affects USP1
2
|
WDR48 affects WD40 domain
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2
WDR48 affects Ser313
|
2
WD40 domain affects WDR48
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2
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1
1
USP1 affects ubiquitination
|
2
USP1 affects transcription, DNA-templated
|
2
USP1 affects response therapeutic enzalutamide
|
2
eidos
"The supportive evidence is as follows : ( i ) USP1 knockdown decreased PC cell proliferation in vitro and tumorigenesis in vivo , and promoted the response to therapeutic agent enzalutamide ; and ( ii ) inhibition of USP1 sensitized cancer cells to therapeutic agent enzalutamide , whereas this effect was blunted by overexpression of KDM4A ."
USP1 affects proliferating cell nuclear antigen PCNA cyclin D1 cyclin E1
|
2
USP1 affects monoubiquitinated-PCNA
|
2
USP1 affects migration cells
|
2
USP1 affects mUb-PCNA
|
2
USP1 affects enzalutamide
|
2
USP1 inhibits enzalutamide. 2 / 2
|
2
reach
"The supportive evidence is as follows : (i) USP1 knockdown decreased PC cell proliferation in vitro and tumorigenesis in vivo, and promoted the response to therapeutic agent enzalutamide; and (ii) inhibition of USP1 sensitized cancer cells to therapeutic agent enzalutamide, whereas this effect was blunted by overexpression of KDM4A."
USP1 affects cisplatin-resistant non-small cell lung cancer
|
2
USP1 affects cell death
|
2
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
USP1 affects Ser313
|
2
USP1 affects Osteosarcoma
|
1
USP1 activates Osteosarcoma. 1 / 2
|
1
USP1 affects MotW
|
2
reach
"Protein localization analyses on truncation MotW variants indeed indicated that the protein’s periplasmic domains, and not its C-terminal cytosolic tail, are essential for the HubP-dependent targeting to the pole (S3J and S3K Fig), whereas protein pulldown experiments and bacterial two-hybrid complementation assays support a physical interaction between HubP and MotW, which could be strengthened by additional binding partners in vivo (S4D and S4E Fig)."
USP1 affects FlhG
|
2
USP1 affects EcRB1
|
2
USP1 affects DNA replication
|
2
USP1 affects DNA damage checkpoint
|
2
USP1 inhibits DNA damage checkpoint. 2 / 2
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2
USP1 affects DNA Damage
|
2
USP1 activates DNA Damage. 2 / 2
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2
USP1 affects BBTB permeability
|
1
1
USP1 affects B-ALL cell apoptosis
|
2
USP1 affects ATPase activity
|
2
Ser313 affects WDR48
|
2
sparser
"Importantly, the results revealed that neither mutant complex is able to catalyze FANCD2 deubiquitination (Fig. xref , lanes 5 and 7) even though, as documented above and in Supplementary Fig. xref , both UAF1 mutants retain all other known biochemical attributes, namely, interactions with RAD51AP1, USP1, and FANCI, and also the ability to enhance the DUB activity of USP1."
sparser
"In the landmark mechanism, a ParA protein serves as an adaptor between a landmark protein and a protein to be localized as described for MinD interacting with MinJ in B. subtilis , ParA interacting with TipN in C. crescentus , and ParA1, FlhG, and ParC interacting with HubP in V. cholerae ."
MotW affects USP1
|
2
reach
"Protein localization analyses on truncation MotW variants indeed indicated that the protein’s periplasmic domains, and not its C-terminal cytosolic tail, are essential for the HubP-dependent targeting to the pole (S3J and S3K Fig), whereas protein pulldown experiments and bacterial two-hybrid complementation assays support a physical interaction between HubP and MotW, which could be strengthened by additional binding partners in vivo (S4D and S4E Fig)."
sparser
"Importantly, the results revealed that neither mutant complex is able to catalyze FANCD2 deubiquitination (Fig. xref , lanes 5 and 7) even though, as documented above and in Supplementary Fig. xref , both UAF1 mutants retain all other known biochemical attributes, namely, interactions with RAD51AP1, USP1, and FANCI, and also the ability to enhance the DUB activity of USP1."
ErGPCR affects USP1
|
2
EcRB1 affects USP1
|
2
sparser
"In the landmark mechanism, a ParA protein serves as an adaptor between a landmark protein and a protein to be localized as described for MinD interacting with MinJ in B. subtilis , ParA interacting with TipN in C. crescentus , and ParA1, FlhG, and ParC interacting with HubP in V. cholerae ."
C-Cdh1 affects USP1
|
2
ATPase activity affects USP1
|
2
1,4-dithiothreitol affects USP1
|
1
1
Μ affects USP1
|
1
reach
"The specificity of SJB was confirmed by various experiments : first, we show that SJB potently and selectively block USP1 activity without inhibiting other DUBs (USP2/USP5/USP7/USP14/UCH37); second, SJB inhibited binding of USP1 with HA-Ub-VS probe, but it did not affect labeling of other DUBs with probe; and third, SJB inhibited USP1, but not USP2 or USP7, triggered cleavage of ubiquitin tetramer chains."
USP1 binds DNMT3A, Cell Hypoxia, thrB, and Circulin-C. 1 / 1
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1
ShUSP1 affects USP1
|
1
Serine affects WDR48
|
1
Resveratrol affects USP1
1
|
PcDNA3.1 affects USP1
|
1
Oxaliplatin affects USP1
1
|
MiR-34a affects USP1
|
1
Juvenile hormone affects USP1
|
1
Hsa-miR-7641 affects USP1
1
|
Hsa-miR-6893-5p affects USP1
1
|
Hsa-miR-6858-3p affects USP1
1
|
Hsa-miR-6821-3p affects USP1
1
|
Hsa-miR-6736-5p affects USP1
1
|
Hsa-miR-6499-3p affects USP1
1
|
Hsa-miR-640 affects USP1
1
|
Hsa-miR-6087 affects USP1
1
|
Hsa-miR-575 affects USP1
1
|
Hsa-miR-558 affects USP1
1
|
Hsa-miR-4722-5p affects USP1
1
|
Hsa-miR-4649-3p affects USP1
1
|
Hsa-miR-4487 affects USP1
1
|
Hsa-miR-25-5p affects USP1
1
|
Hsa-miR-192-5p affects USP1
1
|
Hsa-miR-1262 affects USP1
1
|
HsPCNA affects USP1
|
1
Hexadecanoic acid affects USP1
1
|
Heterodimeric complex affects USP1
|
1
Foci affects WDR48
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1
Fenofibrate affects USP1
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Factor 1 affects USP1
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1
Endosulfan affects USP1
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Coumestrol affects USP1
1
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Copper atom affects USP1
1
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Calcium(2+) affects USP1
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1
Calcium(2+) leads to the phosphorylation of USP1. 1 / 1
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1
reach
"Structures of non-selective DUB inhibitors.Structures of USP1/UAF1 inhibitors.Structures of a representative Novartis USP2 inhibitor and the cathepsin K inhibitor dutacatib.Structures of early USP7 inhibitors from Hybrigenics.USP7 IC50 4.2 μM USP7 IC 50 8.0 μM USP7 IC 50 0.42 μM USP47 IC 50 4.3 μM USP47 IC 50 8.7 μM USP47 IC 50 1.Structures of USP7 inhibitors from Progenra.Structures of recent USP7 inhibitors.Structures of USP8 inhibitors from Hybrigenics.Structures of a USP8 binder and two inhibitors of non-DUB cysteine proteases which advanced to clinical trials."
WDR48 affects serine
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1
WDR48 affects foci
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1
reach
"The specificity of SJB was confirmed by various experiments : first, we show that SJB potently and selectively block USP1 activity without inhibiting other DUBs (USP2/USP5/USP7/USP14/UCH37); second, SJB inhibited binding of USP1 with HA-Ub-VS probe, but it did not affect labeling of other DUBs with probe; and third, SJB inhibited USP1, but not USP2 or USP7, triggered cleavage of ubiquitin tetramer chains."
USP1 binds DNMT3A, Cell Hypoxia, thrB, and Circulin-C. 1 / 1
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1
USP1 affects structural FlhG become active
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1
USP1 affects serine
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1
USP1 affects sensitivity irradiation
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1
USP1 affects robust UV-induced PCNA mono-ubiquitylation
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1
USP1 affects rhythmic flies
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1
USP1 affects response PC cells therapeutic enzalutamide
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1
USP1 affects resistance platinum
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1
USP1 affects protein kinase B signaling
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1
USP1 inhibits protein kinase B signaling. 1 / 1
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1
USP1 affects protein ID1 cells
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1
USP1 affects polyinosinic:polycytidylic acid
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1
USP1 activates polyinosinic:polycytidylic acid. 1 / 1
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1
USP1 affects pevonedistat
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1
USP1 activates pevonedistat. 1 / 1
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1
USP1 affects pcDNA3.1
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1
USP1 affects oncogene-induced senescence
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1
USP1 affects myelin
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1
USP1 affects monoubiquitinated PCNA accumulation ssDNA gaps cells treated HU doses
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1
USP1 affects miR-192-5p
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1
USP1 affects inhibitory miR-194-5p occludin ZO-1 claudin-5 mRNAs
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1
USP1 affects hsPCNA
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1
USP1 affects hMSC
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1
USP1 affects growth migration liver cancer cells
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1
USP1 affects foci
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1
USP1 affects factor 1
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1
USP1 affects effector CD8 T cell
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1
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1
USP1 inhibits cyclobutane-1,1-dicarboxylate(2-). 1 / 1
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1
reach
"Validation screening by using five specific shRNA and gene on four different EOC cell lines (i.e., MDAH, SKOV3, TOV112D, and OV-90), demonstrated that silencing of five genes, namely ATR, BCL2L1 (also known as Bcl-XL), SGK2, USP1, and SFPQ, were able to significantly increase CBDCA response, in at least 3/4 tested cell lines."
USP1 affects chemotaxis
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1
USP1 activates chemotaxis. 1 / 1
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1
USP1 affects c-Myc mRNA
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1
USP1 affects binding AR c-Myc absence R1881
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1
eidos
"Knockdown of USP1 equally reduced the binding between AR and c-Myc enhancer in the presence or absence of R1881 , and the reconstitution of KDM4A partially rescued the effect ( Figure5I ) , suggesting that USP1 not only regulates the binding between AR and c-Myc enhancer by KDM4A , but also by other transcription factors ."
USP1 affects accumulation monoubiquitinated FANCD2
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1
USP1 affects aberrant recruitment DNA polymerase Kappa replication forks
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1
USP1 affects TEER
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1
USP1 affects SflA regulators [11–15]
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1
USP1 affects PCNA cyclin D1 cyclin E1
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1
USP1 affects PC cell
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1
eidos
"The supportive evidence is as follows : ( i ) USP1 knockdown decreased PC cell proliferation in vitro and tumorigenesis in vivo , and promoted the response to therapeutic agent enzalutamide ; and ( ii ) inhibition of USP1 sensitized cancer cells to therapeutic agent enzalutamide , whereas this effect was blunted by overexpression of KDM4A ."
USP1 affects OSTERIX
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1
USP1 affects Neoplastic Stem Cells
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1
USP1 activates Neoplastic Stem Cells. 1 / 1
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1
USP1 affects NSCLC
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1
USP1 affects MAST1-mediated MEK1
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1
USP1 affects KDM4A overexpression
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1
eidos
"Taken together , these results reveal that upregulation of USP1 expression could contribute to KDM4A overexpression in a substantial fraction of human prostate tumors , whereas in other prostate tumors , KDM4A can be activated by other posttranslational regulation , eg downregulation of microRNA-10 was correlated with high expression levels of KMD4A in human prostate tumors.29 Targeting USP1 promotes PC cell response to therapeutic agent enzalutamide Our above findings suggest that targeting the USP1-KDM4A axis could antagonize the growth of PC cells ."
USP1 affects KDM4A PC cells
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1
USP1 affects K63-linked polyubiquitination AKT
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1
USP1 affects K48-linked ubiquitinated-RPS16
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1
USP1 affects ISRE
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1
USP1 affects ID1 p-AKT
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1
USP1 affects H3K27me3
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1
USP1 affects GC Metastasis
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1
USP1 affects FANCI-FANCD2
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1
USP1 affects FANCD2-FANCI
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1
USP1 affects Endoplasmic Reticulum Stress
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1
USP1 inhibits Endoplasmic Reticulum Stress. 1 / 1
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1
USP1 affects Diabetic Nephropathies
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1
USP1 activates Diabetic Nephropathies. 1 / 1
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1
USP1 binds DNMT3A, Cell Hypoxia, thrB, and Circulin-C. 1 / 1
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1
USP1 affects DNA-binding proteins 1–4
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1
USP1 affects Circulin-C
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1
USP1 binds DNMT3A, Cell Hypoxia, thrB, and Circulin-C. 1 / 1
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1
USP1 affects Carcinoma, Hepatocellular
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1
USP1 activates Carcinoma, Hepatocellular. 1 / 1
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1
sparser
"In these cells and based on its suppression by RNAi, this phosphorylated calponin was necessary for the up-regulation of USP1 and HR3 as well as part of the up-regulation of PKC by 20E. In the methoprene-treated cells, the non-phosphorylated calponin bound with USP1 was necessary for methoprene-induced USP1 up-regulation along with that of JHi."
USP1 affects CKN1A
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1
USP1 affects CBDCA response
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1
eidos
"Validation screening by using five-specific shRNA / gene on four different EOC cell lines ( i.e ., MDAH , SKOV3 , TOV112D , and OV-90 ) , demonstrated that silencing of five genes , namely ATR , BCL2L1 ( also known as Bcl-XL ) , SGK2 , USP1 , and SFPQ , were able to significantly increase CBDCA response , in at least 3/4 tested cell lines ."
USP1 affects APC Cdh1
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1
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1
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1
sparser
"Such modifications might modulate the kinetics of targeting and degradation of the newly formed Gln -Usp1 Ct fragment, for example, by altering the steric accessibility of the newly exposed N-terminal region of Gln -Usp1 Ct to the Ntaq1 Nt Q -amidase and downstream components of the Arg/N-end rule pathway."
USP1 affects 20E
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1
USP1 inhibitors affects USP1
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1
Phosphatase affects USP1
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1
Phosphatase activates USP1. 1 / 1
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1
PI3K/Akt/FoxO affects USP1
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1
MicroRNA-192-5p affects USP1
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1
ML-323 MHCC97H SK-Hep-1 cell lines affects USP1
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1
HaloTag-USP1 fusions affects USP1
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1
Enterolactone affects USP1
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EcR-B1 affects 20E
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1
USP1 binds DNMT3A, Cell Hypoxia, thrB, and Circulin-C. 1 / 1
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1
sparser
"In these cells and based on its suppression by RNAi, this phosphorylated calponin was necessary for the up-regulation of USP1 and HR3 as well as part of the up-regulation of PKC by 20E. In the methoprene-treated cells, the non-phosphorylated calponin bound with USP1 was necessary for methoprene-induced USP1 up-regulation along with that of JHi."
CCdh1 affects USP1
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1
Antigen-Presenting Cells affects USP1
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1
Antigen-Presenting Cells inhibits USP1. 1 / 1
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1
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1
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1
sparser
"Such modifications might modulate the kinetics of targeting and degradation of the newly formed Gln -Usp1 Ct fragment, for example, by altering the steric accessibility of the newly exposed N-terminal region of Gln -Usp1 Ct to the Ntaq1 Nt Q -amidase and downstream components of the Arg/N-end rule pathway."
20E affects USP1
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1
(-)-demecolcine affects USP1
1
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(+)-JQ1 compound affects USP1
1
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