IndraLab

Statements

USP1 is ubiquitinated. 7 / 7
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"Consequently S313 phosphorylation plays a crucial role in maintaining protein stability during mitosis as Usp1 is ubiquitinated during G1 by APC/C cdh1 leading to its proteosomal degradation."
25962961
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"Using K11-only mutant ubiquitin, we performed an in vivo ubiquitination assay of USP1 in S/G2 cells depleted of Cdh1. xref shows that USP1 was ubiquitinated (K11-linked) in vivo ."
28604711
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"To further support that USP1 is ubiquitinated via APC/C Cdh1 , we reconstituted the ubiquitination of USP1 in vitro."
21768287
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"To facilitate visualization of USP1 ubiquitination, HEK293T cells were transiently transfected with HA-tagged ubiquitin prior to treatment with PEITC, bortezomib or a combination of PEITC and bortezomib."
28881649
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"Although the depletion of Cdh1 increased USP1 expression, the levels of ubiquitinated USP1 were significantly reduced, suggesting that the formation of K11 linkage on USP1 was highly dependent on the function of Cdh1."
28604711
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"It was reported to mediate ubiquitination and degradation of USP1 to allow NER repair of UV-induced DNA damage xref , Rad17 for checkpoint termination at the end of UV-induced DNA damage response xref and Plk1 to prevent precocious mitotic entry xref ."
28604711
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"USP1 was efficiently ubiquitinated only when Cdh1 was present and a specific E2 combination was used (see Materials and methods section; xref and)."
21768287