IndraLab

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USP1 deubiquitinates EZH2. 13 / 13
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"Accordingly, depletion of endogenous USP1 using two independent shRNAs promotes EZH2 ubiquitination in GSC11 and U87MG cells (Fig.3D and Fig.S3B)."
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"Overexpression of wild-type USP1 notably reduced the ubiquitination of EZH2 (lane 3, Fig.3C), and this effect was enhanced by further expressing WDR48, an USP1-binding partner enhancing the activity of USP1 (lane 4, Fig.3C)."
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"Of those four DUBs, only USP1 significantly reduced the ubiquitination of EZH2 protein (Fig.S2B), indicating that USP1 may act as a deubiquitinase of EZH2."
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"USP1 interacts with and deubiquitinates EZH2 directly.."
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"Moreover, EZH2 is deubiquitinated in vitro by USP1/WDR48."
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"Furthermore, β-catenin could transcriptionally activate USP1 which in turn deubiquitinates and stabilizes EZH2."
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"beta-catenin/TCF4 activated transcription of the deubiquitinase USP1, which then interacted with and deubiquitinated EZH2 directly."
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"To address the question whether USP1 deubiquitinates EZH2 directly, ubiquitinated EZH2 was purified from HEK293T cells and incubated with USP1 and WDR48 proteins."
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"We found that β-catenin induced the expression of USP1, which then interacted and deubiquitinated EZH2 directly to suppress the ubiquitin-proteasomal degradation of EZH2."
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"In vitro deubiquitylation of EZH2 by USP1 was performed as described previously."
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"USP1 and EZH2 interact with each other and overexpression of USP1 diminishes the ubiquitination of EZH2, which is further enhanced by the overexpression of USP1 binding partner, WDR48."
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"We found that USP1/WDR48 decreased EZH2 ubiquitination, and the efficiency was increased with the amount of USP1/WDR48 proteins that were added to the reactions (Fig.3E)."
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"Thus, these results strongly support that USP1 interacts with and deubiquitinates EZH2 directly."
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