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"In U2OS cells, USP1 depletion was consistently associated with a clear reduction in the steady-state level of the CHK1 protein (Fig.  xref A and B)."

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"USP1 depletion was associated with a consistent and significant reduction in the level of phosphorylated S345-CHK1 in both U2OS and HeLa cells (Fig.  xref B–E and)."