IndraLab

Statements

USP1 binds SHPRH and NFAT5. 8 / 8
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"Our previous study demonstrated that TonEBP interacts with the E3 ubiquitin ligase SHPRH and the deubiquitinase USP1, and that this correlates with PCNA polyubiquitination in response to DNA damage [ xref ]."
32825390
sparser
"This sequential pattern of recruitment to the DNA damage site was consistent with the pattern of interactions of SHPRH and USP1 with TonEBP ( xref E and the bottom of xref F)."
31376680
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"To rule out an indirect interaction between TonEBP and SHPRH or USP1 through DNA, whole-cell extracts were preincubated with ethidium bromide (EtBr, which intercalates DNA) or benzonase, which degrades DNA before coIP."
31376680
sparser
"In these experiments, we identified interactions of TonEBP with SHPRH and USP1."
31376680
sparser
"Preincubation with EtBr or benzonase did not affect the interaction between TonEBP and SHPRH or USP1 ( xref F and xref F)."
31376680
sparser
"To confirm the interaction between TonEBP and SHPRH or USP1, endogenous TonEBP and SHPRH were coimmunoprecipitated ( xref D)."
31376680
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"The regulation of PCNA polyubiquitination by TonEBP could be mediated by the activation of SHPRH or suppression of USP1 because TonEBP interacts with SHPRH and USP1 ( xref D and 1E)."
31376680
sparser
"To test this hypothesis, we investigated the temporal interactions of TonEBP with SHPRH and USP1 after DNA damage."
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