IndraLab

"PLK1 possibly phosphorylates USP1 at S313 and increases the stability of USP1, which may explain the reduction of USP1 after PLK1 inhibitor treatment."

"Our results strongly suggest that Usp1 is directly or indirectly involved in the control of centrosome duplication, and Usp1 overexpression may contribute to oncogenic transformation, in part, by inducing centrosome abnormality and chromosome instability.ResultsOverexpression of Usp1 results in centrosome amplificationTo address the effects of USP1 in centrosome duplication, NIH3T3 cells were stably transfected with empty retroviral vector or the retroviral vector encoding murine wild-type Usp1 (wt-Usp1), and the numbers of centrosomes per mononucleated cell were determined by immunofluorescence microscopy."