IndraLab

Statements

USP1 binds lacZ. 9 / 9
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"Using our established Usp1-LacZ reporter mice, we next examined Usp1 expression patterns in T cells responding to infection."
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"Splenic effector CD4 + T cells from Usp1-LacZ reporter mice infected with LCMV-Armstrong were also analyzed."
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"Congenically distinct naive Usp1-LacZ reporter P14 CD8 + T cells were transferred into mice that were then infected with LCMV."
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"To track the T cell responses in vivo , the Usp1-LacZ reporter mice were crossed to LCMV-GP 33 -specific P14 TCR transgenic mice."
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"Peripheral blood CD8 + T cells compared over the course of infection were examined by flow cytometry for Usp1-LacZ reporter activity ( xref , xref )."
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"Congenically distinct Usp1-LacZ reporter P14 CD8 + T cells were transferred into mice that were then infected with LCMV or 2 different recombinant pathogens, Listeria monocytogenes bacterial strain (Lm-GP 33 ) or recombinant Vaccinia Virus strain (VV-GP 33 )."
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"Usp1-LacZ reporter mice were infected with lymphocytic choriomeningitis virus (LCMV) and peripheral blood CD8 + T cells specific for the immunodominant epitope GP 33 ( xref , xref ) or the subdominant epitope GP 276 ( xref , xref ) were assessed for Usp1 reporter activity over the course of infection."
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"To achieve this, we generated Usp1 conditional knockout (KO) mice by crossing our Usp1-LacZ reporter mouse line to transgenic FLP mice."
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"To ensure this phenomenon was not simply due to differences in the pathogens used for rechallenge, we transferred congenically distinct Usp1-LacZ reporter P14 CD8 + T cells into mice then infected half with LCMV and the other half with Lm-GP 33 ."
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