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Statements

USP1 is phosphorylated. 41 / 48
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"In an analogous manner, USP1 phosphorylation was suggested to be required for complex formation with the activator UAF-1 [75] ."
26073511
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"We further define a mechanism in which RSK1 modulates FLT3 stability through USP1 via proteosome-mediated ubiquitin degradation and identify an additional core dependency in USP1, actionable through pre-clinical agents such as SJB3-019A. Strong co-dependency between FLT3 and USP1 were observed, and it is plausible that RSK1 mediates direct phosphorylation of USP1 or other interacting partners along the ubiquitin-proteasome system."
39592591
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"Increased levels of 20E antagonizes phosphorylation of Akt and instead promotes 20E-responsive gene transcription indirectly by promoting phosphorylation of USP1, which enhances EcR-B1/USP1 binding to ecdysone response elements."
31955616
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"Specifically, we demonstrate that USP1 is phosphorylated in mitosis by cyclin-dependent kinases (Cdks), and that this phosphorylation event may prevent premature degradation of USP1 during normal cell cycle progression."
22101265
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"In contrast, two other known USP1 serine phosphorylations (Ser42 and Ser67) are dispensable with respect to the activity of the USP1/UAF1 complex."
23116119
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"Moreover, 20E induced heterodimeric partner USP1 phosphorylation to interact with the nuclear receptor EcRB1 to form EcRB1/USP1 transcription complex, thereby initiating 20E-related gene transcription[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27209368
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"20E (2 μM) was added into the cells for 30 min to induce the phosphorylation of CDK10 and USP1."
25125388
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"Our observation revealed a regulatory mechanism of USP1’s activity in which a specific phosphorylation in USP1 and the subsequent interaction with a partner protein UAF1 exert the stimulatory effect on USP1 catalytic activity."
23116119
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"Identification of USP1 phosphorylation sites by mass spectrometry."
23116119
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"20E, via GPCRs, induces rapid increase in cellular calcium and phosphorylation of transcription complex proteins USP1 and CDK10 to activate gene transcription xref ."
25728569
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"Mutation analysis of the phosphorylated USP1 residues."
23116119
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"Moreover, 20E induced USP1 and CDK10 phosphorylation."
25728569
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"Further experiments showed that USP1 phosphorylation is essential for interaction with UAF1 and its protease activity ( xref )."
29362236
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"By contrast, lambda protein phosphatase (λPPase) treatment degraded USP1 and CDK10 phosphorylation."
25728569
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"ErGPCR-2 knockdown repressed 20E-induced USP1 and CDK10 phosphorylation ( xref ), which are essential for the formation of EcRB1/USP1 transcription complex to initiate gene transcription in 20E signaling xref xref ."
25728569
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"In response to DNA damage, USP1 is phosphorylated and undergoes an inactivating self-cleavage through recognition of an internal ubiquitin-like domain ( Huang et al., 2006 )."
18082599
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"Given that Ca 2+ may trigger PKC activation [20] and further protein phosphorylation [25] , we examined the involvement of PKC in the phosphorylation of CDK10 and USP1, because these proteins are demo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25125388
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"20E via GPCR-, PLC-, Ca 2+ -, and PKC-signaling mediates USP1 phosphorylation for gene transcription xref ."
25728569
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"20E via PKC regulates CDK10 xref and USP1 phosphorylation to form the EcR/USP transcription complex xref ."
25728569
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"Recent work showed that phosphorylation of USP1 by kinase CDK1 promotes the complex formation between USP1 and UAF1( xref )."
23797609
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"20E-induced intracellular Ca 2+ increase causes the phosphorylation and acetylation of USP1, allowing it to bind EcRB1, which then regulates the transcription of downstream genes such as HR3 and BrZ7 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
29129205
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"USP1 phosphorylation via cyclin-dependent kinases (Cdks) might have a role in prevention of premature degradation of USP1 during cell cycle progression [ xref ]."
28659380
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"After phosphorylation of USP1, the complex translocated to the nucleus, where the recruitment of the Fanconi anemia protein FANCD2 and PCNA substrates mediated by a SUMO-like domain occurs in response to DNA damage ( xref ; xref ; xref ; xref )."
34177595
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"20E via Gαq regulated the increase in cytosolic Ca 2+ and phosphorylation of CDK10 and USP1, which were responses to gene transcription and metamorphosis in the 20E pathway."
25125388
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"The non-phosphorylated HaCal then binds with the non-phosphorylated USP1, which might promote the interaction between USP1 and other proteins such as Met1 in the JH signaling cascade ( xref )."
21625546
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"20E-induced Ca 2+ signaling can activate protein kinase C, which regulates USP1 phosphorylation and the interaction with EcRB1 to induce 20E pathway gene transcription [58] ."
29129205
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"These results suggest that 20E activates PLCG1 via the ErGPCR and Src family kinases to regulate Ca(2+) influx and PKC phosphorylation of USP1 to subsequently modulate gene transcription for metamorphosis."
24692553
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"The PKC for Gαq phosphorylation might differ from the PKC regulating USP1 phosphorylation."
25125388
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"A link between the DDR and EMT has been established, because cisplatin exposure of ovarian cancer cells results in USP1 phosphorylation by ATM and ATR, the two core kinases of the DDR; such a phosphorylation is required for binding to Snail, a process activating stemness feature that can ultimately induce cell reprogramming to favor survival."
39430244
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"ATM/ATR phosphorylate USP1 and favor USP1/Snail interaction in response to CDDP treatment."
31086816
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"Phosphorylation of USP1 has been shown as a key regulator in its interaction with the protein partner USP1-associated factor 1 (UAF1)( xref )."
23797609
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"No S/TQ phosphorylation on Snail was detectable, while USP1 was considerably phosphorylated on S/TQ motif(s), particularly after CDDP treatment, both in exogenously overexpressed and in endogenous protein (fig."
31086816
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"To evaluate whether USP1 phosphorylation after CDDP treatment was directly operated by ATM/ATR, we treated OC cells with CDDP in combination with selective inhibitors of ATM (Ku55933) and/or ATR (VE-821) and performed co-IP experiments."
31086816
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"Combined ATM and ATR inhibition strongly reduced S/TQ phosphorylation of endogenous USP1 in OC cells (fig."
31086816
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"Further, USP1 phosphorylation was important for USP1-mediated stabilization of Snail, as inhibition of ATM/ATR reduced CDDP-induced Snail protein up-regulation ( xref and fig."
31086816
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"We observed that under basal condition, USP1 is phosphorylated on four serines, but only S42 phosphorylation was strongly increased after CDDP treatment ( xref , fig."
31086816
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"ATR-mediated phosphorylation of the USP1-UAF1 complex and of its substrate, ubiquitylated FANCI-D2, has also been shown to regulate the formation of the enzyme-substrate complex for efficient Ub removal."
38572758
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"Our data, together with current literature, suggest that platinum-treated cancer cells could use a “tunable” pathway, which starts with the phosphorylation of USP1 by ATM/ATR that induces a mesenchymal status via Snail stabilization necessary for the survival and for initial abdominal colonization."
31086816
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"Our observations, linking the activation of ATM/ATR to USP1 phosphorylation and Snail stabilization, again support the possibility that CDDP itself could represent one of these key environmental signals."
31086816
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"At later times, when DDR is concluded, the decrease of USP1 phosphorylation and of Snail protein levels restores the epithelial status of cancer cells and allows their massive growth at the metastatic sites."
31086816
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"For example, the CDK1-mediated serine phosphorylation of USP1 disrupts the interaction between USP1 and UAF1."
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