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USP1 binds circ. 54 / 54
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"Researchers verified that the expression of some circular isoforms, such as the circRNAs derived from the DCC (deleted in colorectal cancer) gene, diversified across a range of human tissues and was not correlated with its cognate linear mRNA expression. xref Because of the up‐regulation of circ‐USP1 in GECs, we are interested to explore whether the dysregulation of circ‐USP1 was associated with the regulation of BTB function."
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"Additionally, silencing circ‐USP1 reduced kidney injuries caused by renal allografts in mice."
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"To further explore whether circ‐USP1 interact with miR‐194‐5p to regulate BTB permeability, antagomiR‐194‐5p was transfected into the stable circ‐USP1 knockdown GECs."
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"Circ‐USP1 bound to miR‐194‐5p to regulate BTB permeability."
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"Additionally, silencing circ‐USP1 in mice protected them from kidney injury following a renal allograft."
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"Silencing circ‐USP1 reduced injury in the kidney of mice that received renal allografts."
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"Silencing circ‐USP1 reduced expression of DNMT3A in the kidney of mice that received renal allografts."
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"Additionally, the over‐expression of circ‐USP1 remarkably reduced miR‐194‐5p expression in HK‐2 cells of the allograft group than that in the allograft+si‐circ‐USP1 group."
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"Moreover, the study showed that miR‐194‐5p is a target for circ‐USP1."
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"Bioinformatics analysis showed that miR‐194‐5p might be a target for circ‐USP1, as can be seen from Figure  xref ."
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"Knockdown of the cellular expression of circ-USP1, circRNA_001160, cDENND4C disrupted the barrier integrity, increased barrier permeability accompanied by reduced tight junction-related proteins claudin-5, occludin, and ZO-1 by targeting miR-194-5p/FLI1 (Friend leukemia virus integration 1), miR-195-5p/ETV1 (Ets variant gene 1), and miR-577, respectively [165] [166] [167] ."
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"The expression of circ‐USP1 increased, while that of miR‐194‐5p decreased in renal allografts."
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"Gao et al found that silencing circ‐USP increased permeability of the blood‐tumour barrier by decreasing tight junction‐related protein expression in cerebral microvascular endothelial cells in glioma. xref In this study, it was shown that silencing the circ‐USP1 protects renal tubule cells from apoptosis."
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"It was also shown that down‐regulation of circ‐USP1, through si‐circ‐USP1 delivery to mice, prevented kidneys from injuries in renal allografts."
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"Compared with the group on day 0, the circ‐USP1 expression was significantly elevated in a time‐dependent manner."
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"Compared with the control probe group, the expression of miR‐194‐5p and circ‐USP1 was significantly higher in the circ‐USP1 probe group in Figure  xref ."
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"To further elucidate the association between DNMT3A and circ‐USP1, expression of DNMT3A was examined in the kidneys of mice."
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"The results of RT‐PCR indicated that circ‐USP1 significantly increased in a time‐dependent manner at day 0.5, day 1 and day 3 post‐engraftment."
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"Therefore, this study sought to investigate the role of circ‐USP1 in the pathogenesis of early renal allografts."
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"The expression of circ‐USP1 elevated and miR‐194‐5p reduced in renal allografts."
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"Circ‐USP1 functions as a competing endogenous RNA for miR‐194‐5p."
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"In addition, our research group has also identified miR‐379‐3p and miR‐411‐3p as downstream targets of circ‐USP1, and we continue to conduct related experimental studies in the future."
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"Furthermore, it was observed that circular RNA USP1 (circ‐USP1) [165], circRNA_001160 [166], and circular RNA DENND4C (cDENND4C) [167] were also deeply involved and markedly upregulated in GECs."
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"Knockdown of the cellular expression of circ‐USP1, circRNA_001160, cDENND4C disrupted the barrier integrity, increased barrier permeability accompanied by reduced tight junction-related proteins claudin-5, occludin, and ZO-1 by targeting miR-194-5p/FLI1 (Friend leukemia virus integration 1), miR-195-5p/ETV1 (Ets variant gene 1), and miR-577, respectively [165–167]."
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"Finally, reduced expression of circ‐USP1 was accompanied by increased miR‐194‐5p and decreased DNMT3A expression in kidneys."
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"In conclusion, circ‐USP1 functions as a competing endogenous RNA for miR‐194‐5p."
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"These findings, therefore, reveal that miR‐194‐5p might be a target for circ‐USP1."
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"First of all, it was the first study to examine the role of circ‐USP1 in injuries caused by kidney transplantation."
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"Finally, it was shown that silencing circ‐USP1 reduced DNMT3A expression in the kidney of mice that received renal allografts."
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"In this study, the expression and role of circ‐USP1, miR‐194‐5p and DNMT3A in kidney injury induced by hypoxia in acute renal allografts were investigated."
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"As presented in Figure  xref , compared with the control group, the expression of circ‐USP1, DNMT3A and the rate of TUNEL‐positive cells were significantly up‐regulated in the H/R group and the H/R+Mock group, while the expression of miR‐194‐5p was significantly down‐regulated."
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"Although recent reports indicate that circ‐USP1 is involved in the dysregulation of endothelial cell functions, xref its role in kidney diseases remains largely unclear."
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"The interaction between miRNA and circRNA may competitively affect miRNA‐mediated regulation of their target genes. xref , xref , xref In oral squamous cell carcinoma cells, circ_100290 plays oncogene function by binding with miR‐29b. xref We demonstrated that circ‐USP1 may interact with miR‐194‐5p and function as an endogenous miR‐194‐5p sponge."
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"The results showed that the expression of circ‐USP1 was elevated in renal allografts in a time‐dependent manner."
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"Silencing circ-USP1 protects the renal tubular from kidney injury induced by hypoxia via modulating miR-194-5p/DNMT3A axis in acute renal allografts."
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"Thereafter, the association between miR‐194‐5p, circ‐USP1 and DNMT3A was confirmed using a combination of bioinformatics and the luciferase reporter gene assay."
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"They bind to the 3' non‐coding region (3' UTR) of the target gene through their seed sequence, leading to degradation or inhibition of post‐transcriptional translation. xref miRNAs serve as vital regulatory roles in physiological processes including cell differentiation, proliferation and apoptosis. xref Extensive research indicates that miRNAs are closely associated with the occurrence of AR. xref , xref , xref Moreover, recent evidence shows that miR‐194‐5p is involved in the progression of various diseases including acute myeloid leukaemia, clear cell kidney carcinoma and hepatocellular carcinoma. xref , xref , xref A previous study showed that miR‐194‐5p was a target of circ‐USP1 and both were involved in regulating the permeability of the blood‐tumour barrier. xref Nevertheless, to the best of our knowledge, the role of miR‐194‐5p and circ‐USP1 in the progression of kidney injury induced by hypoxia in acute renal allografts remains unknown."
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"To examine the vital role of circ‐USP1 in mice renal allografts, the protein was delivered to the animals."
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"The HE results and tubular damage scores showed that injury to the tubules significantly reduced after silencing circ‐USP1 (Figure  xref )."
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"This finding is in line with that of Gao et al , showing that circ‐USP1 modulated the permeability of the blood‐tumour barrier through a miR‐194‐5p/FLI1‐mediated pathway. xref Another study indicated that lower expression of miR‐194‐5p and higher expression of MALAT1, ACVR2B were related with advanced TNM stage, larger tumour size (≥4 cm) and poor prognosis of patients with clear cell kidney carcinoma. xref Additionally, DNMT3A was demonstrated to target miR‐194‐5p and silencing circ‐USP1 reduced DNMT3A expression in the kidney of mice that had a renal allograft."
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"Effects of circ‐USP1 and miR‐194‐5p on the expression of circ‐USP1, miR‐194‐5p and DNMT3A in H/R‐induced HK‐2 cells."
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"Our study indicated that the expression of circ‐USP1 elevated, while that of miR‐194‐5p reduced in renal allografts in a time‐dependent manner."
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"Moreover, miR‐194‐5p was a target for circ‐USP1, and DNMT3A was a target of miR‐194‐5p."
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"Secondly, it is the first research to explore the relationship between circ‐USP1, miR‐194‐5p and DNMT3A in kidney injury induced by hypoxia in acute renal allografts."
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"Additionally, compared with the H/R group and H/R+Mock group, the expression of circ‐USP1 and DNMT3A and the rate of TUNEL‐positive cells were remarkably decreased, while the expression of miR‐194‐5p was remarkably increased in the H/R+si‐circ‐USP1 group."
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"Notably, the expression of miR‐194‐5p, a previously reported circ‐USP1 target, xref decreased in diseased kidneys."
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"Therefore, this finding indicates that circ‐USP1 may be considered as a vital therapeutic target in kidney injury."
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"Additionally, lower circ‐USP1 expression was accompanied by higher miR‐194‐5p and lower DNMT3A expression in the kidney."
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"To clarify which miRNAs might bind to circ‐USP1, we assessed putative miRNA‐binding sites on the circ‐USP1 sequence using CircInteractome database."
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"Additionally, the expression of circ‐USP1, miR‐194‐5p and DNMT3A mRNA was detected through qPCR."
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"Circ‐USP1 bound to miR‐194‐5p."
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"Bioinformatic prediction and luciferase assay indicated that circ‐USP1 bound to miR‐194‐5p and suppressed its activity."
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"miR‐194‐5p was a target for circ‐USP1."
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"Moreover, the expression of circ‐USP1 was remarkably down‐regulated in the si‐circ‐USP1 group compared with the mock group, as displayed in Figure  xref ."
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