IndraLab
Statements
USP4 is modified
2
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1
55
3
USP4 is phosphorylated.
2
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29
3
sparser
"According to a previous study by Iyengar [ xref ], Akt promotes USP4 to enter the cell membrane and cytoplasm to phosphorylate USP4 and regulate its subcellular localization, and Akt phosphorylation of USP4 occurs by combining with other de-ubiquitinating enzymes (DUBs) to de-ubiquitinate the TGF-β receptor [ xref ]."
sparser
"As aforementioned, phosphorylated USP4 can relocate from nucleus to cytoplasm or cell membrane where USP4 deubiquitylates TGF-β receptor I (TβRI) and stabilizes its expression to activate TGF-β signaling pathway to induce R-Smads phosphorylation and then R-Smads are imported into the nucleus to regulate targeted genes expression [ xref ]."
USP4 is ubiquitinated.
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22
sparser
"We therefore assessed ubiquitylation of GFP-USP4 WT, GFP-USP4 CD, and GFP (assessment of endogenous USP4 ubiquitylation events could more directly address the physiological nature of such modifications but was technically not feasible; data not shown) by co-expressing these with human influenza hemagglutinin-epitope-tagged ubiquitin (HA-Ub) and immunoprecipitating ubiquitylated proteins with an HA antibody in the presence of 1 M NaCl."
sparser
"Our mapping of USP4 cysteine ubiquitylations and our observation that such ubiquitylations are labile under reducing conditions, highlight how cysteine ubiquitylation and deubiquitylation might occur more generally, at least within the USP-DUB ubiquitin protease family, many of which contain zinc-binding cysteine motifs ( xref )."
USP4 is methylated.
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2
USP4 is dephosphorylated.
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2
USP4 is produced.
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1
reach
"In addition, the relationship between hsa_circ_0001326 and hsa-miR-136-5p, or between hsa-miR-136-5p and USP4 are also need further verify.In brief, we found luteolin promoted M2 polarization and inhibited M1 polarization by upregulating the expression of hsa_circ_0001326, which then mediated the expression of hsa-miR-136-5p and USP4."
sparser
"In view of the data presented here and previous reports, we propose a working model ( xref ), in which that TNFα rapidly induces Lys63-linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63-linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFα-induced Lys63-linked TAK1 polyubiquitination and TAK1-mediated IKK/NF-κB activation."
reach
"In view of the data presented here and previous reports, we propose a working model (XREF_FIG), in which that TNFalpha rapidly induces Lys63 linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63 linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFalpha induced Lys63 linked TAK1 polyubiquitination and TAK1 mediated IKK and NF-kappaB activation."
"Using a functional genomic approach, we have identified ubiquitin-specific peptidase 4 (USP4) as a deubiquitinase for TAK1."
reach
"Although it has been reported that USP4 deubiquitinates TAK1 and negatively regulates TNF- and IL-1-induced activation of NF-kappaB, how TAK1 ubiquitination is regulated in adaptive immune cells such as T cells and whether such a regulation regulates T cell mediated immune response remain unknown."
reach
"In view of the data presented here and previous reports, we propose a working model (XREF_FIG), in which that TNFalpha rapidly induces Lys63 linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63 linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFalpha induced Lys63 linked TAK1 polyubiquitination and TAK1 mediated IKK and NF-kappaB activation."
reach
"Molecular analysis revealed that USP4 deficiency augmented the activation of the transforming growth factor beta activated kinase 1 (TAK1)-(JNK1/2)/P38 signaling in response to hypertrophic stress, and blockage of TAK1 activation abolished the pathological effects of USP4 deficiency in vivo."
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
reach
"In the present study, we found that the USP4 and beta-catenin axis was involved in metastatic potential through USP4 mediated stabilization of beta-catenin and that knockdown of USP4 and beta-catenin suppressed the metastatic potential including clonogenicity, migration, and invasion, and induced MET by downregulating ZEB1 expression."
reach
"In addition to these newly identified nuclear functions, both USP4 and USP15 are well known to function in the cytosol, i.e. USP4 modulates the Wnt and beta-catenin, NF-kappaB, p53 and TGF-beta signaling pathways while USP15 performs functions in the TGF-beta receptor and NF-kappaB signaling pathways."
sparser
"However, when ALAL-1 was depleted, the SART3-mediated nuclear translocation of USP4 was partially impaired ( xref ), while the interaction between SART3 and USP4 was not affected by depletion of ALAL-1 ( xref ), indicating that ALAL-1 is involved in USP4 relocalization without impairing SART3-USP4 interaction in bulk."
reach
"While it has been reported that overexpressed SART3 causes nuclear localization of exogenously expressed USP4, we found that SART3 depletion from U2OS cells did not detectably affect the nuclear and cytoplasmic distribution of endogenous USP4 (data not shown), suggesting that USP4 nuclear targeting might be mediated by multiple mechanisms."
USP4 affects cell population proliferation
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34
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USP4 inhibits cell population proliferation.
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14
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USP4 activates cell population proliferation.
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20
sparser
"However, when ALAL-1 was depleted, the SART3-mediated nuclear translocation of USP4 was partially impaired ( xref ), while the interaction between SART3 and USP4 was not affected by depletion of ALAL-1 ( xref ), indicating that ALAL-1 is involved in USP4 relocalization without impairing SART3-USP4 interaction in bulk."
sparser
"Although it seems complicated to determine whether a DUB cleaves with endo-or exocleavage activity, several studies have activity, have also been reported to affect the nuclear localization of DUBs. [49] [50] [51] [52] [53] To illustrate, phosphorylation of USP4 by protein kinase B (PKB, also known as AKT) results in its redistribution from the nucleus to the cytoplasm, which enables USP4 to reach the cell membrane and deubiquitinate the transforming growth factor-(TGF-) receptor I (T R-I). [52] Furthermore, localization can also be indirectly modulated by changing the protein interactions that a DUB engages in ref. [25] ."
reach
"According to a previous study by Iyengar [XREF_BIBR], Akt promotes USP4 to enter the cell membrane and cytoplasm to phosphorylate USP4 and regulate its subcellular localization, and Akt phosphorylation of USP4 occurs by combining with other de-ubiquitinating enzymes (DUBs) to de-ubiquitinate the TGF-beta receptor [XREF_BIBR]."
reach
"However, in breast cancer, USP4 was also recognized as a tumour suppressor for its up-regulatory effect on programmed cell death 4 (PDCD4) to restrain tumour growth.20 Moreover, USP4 was able to target TRAF2 and TRAF6 for deubiquitination and thereafter inhibited TNFalpha induced cancer cell migration.34 In the present study, we proved that USP4 expression was drastically increased in melanoma."
sparser
"Recent studies have shown that USP4 serves as a critical control to downregulate NF-κB activation through deubiquitinating TAK1, TRAF2 and TRAF6. xref , xref It has also been reported that HDAC2 could inhibit NF-κB activation. xref , xref In our study we found that HDAC2 could reduce TNFα-induced acetylation of RelA in H1299 cells, but HDAC2 could not interact with RelA ( xref ), consistent with previous studies. xref Therefore, we explored the possibility that USP4 inhibits NF-κB transcriptional activity through HDAC2 using an NF-κB-dependent luciferase reporter gene assay."
reach
"In addition to these newly identified nuclear functions, both USP4 and USP15 are well known to function in the cytosol, i.e. USP4 modulates the Wnt and beta-catenin, NF-kappaB, p53 and TGF-beta signaling pathways while USP15 performs functions in the TGF-beta receptor and NF-kappaB signaling pathways."
reach
"Recently, it has been reported that USP4 could antagonize p53 signalling through its deubiquitinating activity.15, 18 Given that Bax and Bcl2 are 2 canonical downstream regulators of p53 in mediating cell apoptosis and were regulated by USP4 in response to cisplatin treatment, we speculated that pro survival effect of USP4 in response to cisplatin treatment may be associated with p53 signalling pathway."
reach
"In addition to these newly identified nuclear functions, both USP4 and USP15 are well known to function in the cytosol, i.e. USP4 modulates the Wnt and beta-catenin, NF-kappaB, p53 and TGF-beta signaling pathways while USP15 performs functions in the TGF-beta receptor and NF-kappaB signaling pathways."
reach
"Notably, studies have also indicated functions for USP4 in regulating growth factor signalling by the Toll-like receptor/IL1 pathway [88] , TGFb receptor type I [89, 90] , TNFa receptor [91] , growth factoractivated kinase regulation [92] and Wnt signalling [93] , making USP4 a prime target for further evaluation as an oncology drug target with strong potential in DDR contexts.Depletion of USP5 has been reported to cause accumulation of nuclear p53 and increase p53 transcriptional activity."
reach
"USP4, for example, interacts directly with end resection factors CtIP and MRN [172], USP21 deubiquitinates and stabilizes BRCA2 to promote RAD51 binding and successful HR [173], and USP10 deubiquitinates and stabilizes p53 to promote its nuclear localization and apoptosis in response to DSBs [174]."
sparser
"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."
sparser
"In view of the data presented here and previous reports, we propose a working model ( xref ), in which that TNFα rapidly induces Lys63-linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63-linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFα-induced Lys63-linked TAK1 polyubiquitination and TAK1-mediated IKK/NF-κB activation."
reach
"In view of the data presented here and previous reports, we propose a working model (XREF_FIG), in which that TNFalpha rapidly induces Lys63 linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63 linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFalpha induced Lys63 linked TAK1 polyubiquitination and TAK1 mediated IKK and NF-kappaB activation."
reach
"Molecular analysis revealed that USP4 deficiency augmented the activation of the transforming growth factor beta activated kinase 1 (TAK1)-(JNK1/2)/P38 signaling in response to hypertrophic stress, and blockage of TAK1 activation abolished the pathological effects of USP4 deficiency in vivo."
reach
"Conversely, whereas USP4 and USP15 target p53 inhibiting ligases ARF-BP1 [XREF_BIBR] and MDM2 [XREF_BIBR], respectively, USP11 stabilizes p53 [XREF_BIBR] as well as several other tumor suppressors including PML [XREF_BIBR], BRCA2 [XREF_BIBR] and Mre11 complex members MRE11 & RAD50 [XREF_BIBR]."
USP4 affects Neoplasm Invasiveness
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1
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USP4 activates Neoplasm Invasiveness.
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1
21
USP4 inhibits Neoplasm Invasiveness.
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1
USP4 inhibits Neoplasm Invasiveness. 1 / 1
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1
reach
"Collectively, our results support the concept that dysregulation of miR-148a is associated with the poor prognosis of HCC and may account for the tumor progression to advanced stages, and that, of the newly identified targets, USP4 overexpression may contribute to HCC progression towards more aggressive feature presumably by facilitating TGF-beta signaling pathways, growth advantage and migrating capability."
reach
"In addition to these newly identified nuclear functions, both USP4 and USP15 are well known to function in the cytosol, i.e. USP4 modulates the Wnt and beta-catenin, NF-kappaB, p53 and TGF-beta signaling pathways while USP15 performs functions in the TGF-beta receptor and NF-kappaB signaling pathways."
reach
"In this context, to achieve a certain biological outcome, the same DUB can regulate the pathway at different levels (e.g., USP15 and USP4 promote signaling by targeting the TGF-beta receptor and the effector SMADs) or multiple DUBs can act on the same target (e.g., USP15 and OTUB1 promote signaling through stabilizing R-SMADs)."
reach
"However, in breast cancer, USP4 was also recognized as a tumour suppressor for its up-regulatory effect on programmed cell death 4 (PDCD4) to restrain tumour growth.20 Moreover, USP4 was able to target TRAF2 and TRAF6 for deubiquitination and thereafter inhibited TNFalpha induced cancer cell migration.34 In the present study, we proved that USP4 expression was drastically increased in melanoma."
sparser
"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."
USP4 affects Neoplasm Metastasis
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USP4 activates Neoplasm Metastasis.
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USP4 inhibits Neoplasm Metastasis.
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2
USP4 affects cell migration
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USP4 activates cell migration.
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USP4 inhibits cell migration.
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USP4 inhibits cell migration. 5 / 5
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5
reach
"However, in breast cancer, USP4 was also recognized as a tumour suppressor for its up-regulatory effect on programmed cell death 4 (PDCD4) to restrain tumour growth.20 Moreover, USP4 was able to target TRAF2 and TRAF6 for deubiquitination and thereafter inhibited TNFalpha induced cancer cell migration.34 In the present study, we proved that USP4 expression was drastically increased in melanoma."
USP4 affects apoptotic process
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13
USP4 activates apoptotic process.
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USP4 inhibits apoptotic process.
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3
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3
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reach
"In addition to these newly identified nuclear functions, both USP4 and USP15 are well known to function in the cytosol, i.e. USP4 modulates the Wnt and beta-catenin, NF-kappaB, p53 and TGF-beta signaling pathways while USP15 performs functions in the TGF-beta receptor and NF-kappaB signaling pathways."
Ubiquitin binds peptidase activity and USP4. 1 / 1
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1
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
sparser
"Since our data showed that Rheb ubiquitination was inhibited by EGF treatment (Fig. xref ), we examined whether the interaction between USP4 and Rheb was affected by EGF treatment and found that EGF treatment promoted their interaction at both endogenous and exogenous level (Fig. xref and Supplementary information, Fig. xref )."
reach
"Specifically, up-regulated USP4 potentiated the growth and invasion of colorectal cancer though deubiquitination and stabilization of PRL-3.19 In addition, USP4 transduced Akt activation to TGF-beta signalling by deubiquitinating and stabilizing TGF-beta type I receptor, thus augmented breast cancer cell invasion and migration.33 These studies demonstrate USP4 as a powerful tumour promoter and an important determinant for canonical oncogenic signalling."
sparser
"The characteristic pattern [ xref ] of ubiquitylated AQP2 was observed in the immunoprecipitated samples, as shown in xref A. Incubation of immunoprecipitated AQP2 with enzymatically active USP4, but not active USP30, significantly decreased the levels of ubiquitylated AQP2 compared to the control condition, as shown in xref B. This data indicates that a direct and functional interaction between USP4 and AQP2 can occur in vitro to modulate AQP2 ubiquitylation."
sparser
"USP4 and AQP2 were detected in samples immunoprecipitated using either anti-AQP2 or anti-USP4, confirming that a USP4 and AQP2 interaction occurs in vivo, as shown in xref C. Similar to the cultured cell experiments, prior stimulation of the tubule suspensions with dDAVP had no clear effect on the USP4-AQP2 interaction, as shown in xref C."
sparser
"In mpkCCD14 cells, USP4 could be immunoprecipitated with an anti-AQP2 antibody, and prior treatment with dDAVP had no clear effect on the interaction, as shown in xref A. Similar results were obtained in MDCK-hAQP2 cells, as shown in xref B, with an interaction between AQP2 and USP4 observed under control conditions, or after treatment of cells with the adenylate cyclase activator forskolin (MDCK-hAQP2 cells have limited response to VP)."
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
USP4 activates USP4.
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4
reach
"Furthermore, gastrodin significantly upregulated the expression of USP4 and increased the binding of USP4 to the insulin receptor.Considering the importance of the PI3K-Akt pathway in the pathogenesis of T2DM, we detected the effects of gastrodin on the PI3K-Akt pathway to further explore the underlying mechanism of gastrodin in ameliorating insulin resistance."
USP4 increases the amount of USP4.
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2
USP4 phosphorylates USP4.
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1
sparser
"To confirm complex formation of endogenous components, proximity ligation assay (PLA) was performed using the breast cancer cell line MDA-231-BM, known to produce high levels of hyaluronan. xref Using antibodies against HAS2 and USP4 or USP17, reactive signals in the form of red dots indicating interactions between endogenous HAS2 and USP17, and HAS2 and USP4, were observed; only a low background level of dots was seen when each antibody was used alone, or in the fixation control without antibody ( xref )."
"USP17 efficiently removed polyubiquitination, whereas USP4 preferentially removed monoubiquitination of 6myc-HAS2.;The deubiquitinating enzymes USP4 and USP17 target hyaluronan synthase 2 and differentially affect its function;USP17 and USP4 differently affect HAS2 ubiquitination, and the stability and function of HAS2"
sparser
"The characteristic pattern [ xref ] of ubiquitylated AQP2 was observed in the immunoprecipitated samples, as shown in xref A. Incubation of immunoprecipitated AQP2 with enzymatically active USP4, but not active USP30, significantly decreased the levels of ubiquitylated AQP2 compared to the control condition, as shown in xref B. This data indicates that a direct and functional interaction between USP4 and AQP2 can occur in vitro to modulate AQP2 ubiquitylation."
sparser
"USP4 and AQP2 were detected in samples immunoprecipitated using either anti-AQP2 or anti-USP4, confirming that a USP4 and AQP2 interaction occurs in vivo, as shown in xref C. Similar to the cultured cell experiments, prior stimulation of the tubule suspensions with dDAVP had no clear effect on the USP4-AQP2 interaction, as shown in xref C."
sparser
"In mpkCCD14 cells, USP4 could be immunoprecipitated with an anti-AQP2 antibody, and prior treatment with dDAVP had no clear effect on the interaction, as shown in xref A. Similar results were obtained in MDCK-hAQP2 cells, as shown in xref B, with an interaction between AQP2 and USP4 observed under control conditions, or after treatment of cells with the adenylate cyclase activator forskolin (MDCK-hAQP2 cells have limited response to VP)."
sparser
"Since our data showed that Rheb ubiquitination was inhibited by EGF treatment (Fig. xref ), we examined whether the interaction between USP4 and Rheb was affected by EGF treatment and found that EGF treatment promoted their interaction at both endogenous and exogenous level (Fig. xref and Supplementary information, Fig. xref )."
USP4 affects cell growth
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USP4 activates cell growth.
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USP4 inhibits cell growth.
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USP4 inhibits cell growth. 3 / 3
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sparser
"Previous studies have shown that USP4 expression was significantly decreased in breast cancer tissue and that USP4 inhibited breast cancer cell growth through inhibiting PDCD4 degradation. xref USP4 was downregulated in lung adenocarcinoma and served as an independent predictor. xref Some other studies claimed that USP4 might have oncogenic properties."
USP4 affects activation
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USP4 affects E3_Ub_ligase
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2
USP4 binds E3_Ub_ligase.
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USP4 deubiquitinates E3_Ub_ligase.
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USP4 affects homologous recombination
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USP4 activates homologous recombination.
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USP4 activates homologous recombination. 4 / 4
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eidos
"USP4 was found to enhance DNA double-strand breaks repairment , DNA-end resection and homologous recombination via a conversed and specific domain to form a complex with CtIP and the MRE11-RAD50-NBS1 complex which can be abrogated by USP4 auto-deubiquitination on several specific cysteine residues ."
USP4 phosphorylates homologous recombination.
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USP4 affects cell differentiation
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USP4 inhibits cell differentiation.
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USP4 activates cell differentiation.
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reach
"Interestingly, these speculations have been confirmed that HECW1 can promote the proliferation, migration and invasion of cancer cells by inducing the ubiquitination and degradation of SMAD4 (34), while USP4 can mediate the deubiquitination of SMAD4, leading to the expression of apoptotic proteins (35) ."
"We demonstrated that ubiquitin-specific protease (USP) 4 strongly induces activin/BMP signaling by removing the inhibitory monoubiquitination from SMAD4."
reach
"Given the key role of m 6 A mRNA methylation in affecting mRNA stability, it was reasonable to speculate that METTL3 knockdown reduces the m 6 A modification levels in USP4 mRNA and thus increase USP4 mRNA levels by atenuating the inhibitory effect of m 6 A modification on USP4 mRNA stability."
reach
"[49, 50, 51, 52, 53] To illustrate, phosphorylation of USP4 by protein kinase B (PKB, also known as AKT) results in its redistribution from the nucleus to the cytoplasm, which enables USP4 to reach the cell membrane and deubiquitinate the transforming growth factor-beta (TGF-beta) receptor I (TbetaR-I)."
USP4 affects inflammatory response
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USP4 inhibits inflammatory response.
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USP4 activates inflammatory response.
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USP4 affects UBL
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5
reach
"During the course of our studies, we observed that mutating theUSP4 catalytic cysteine to alanine (C311A) to render USP4 enzymatically inactive (" catalytic-dead " [CD]), almost totally abrogated its interactions with CtIP and MRN (XREF_FIG A; note that binding of USP4 to CtIP and RAD50 was not abrogated by the DNA intercalating agent ethidium bromide (EtBr), suggesting that interaction was not mediated by DNA bridging."
reach
"Furthermore, gastrodin significantly upregulated the expression of USP4 and increased the binding of USP4 to the insulin receptor.Considering the importance of the PI3K-Akt pathway in the pathogenesis of T2DM, we detected the effects of gastrodin on the PI3K-Akt pathway to further explore the underlying mechanism of gastrodin in ameliorating insulin resistance."
USP4 affects DNA repair
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3
UBL affects USP4
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5
reach
"During the course of our studies, we observed that mutating theUSP4 catalytic cysteine to alanine (C311A) to render USP4 enzymatically inactive (" catalytic-dead " [CD]), almost totally abrogated its interactions with CtIP and MRN (XREF_FIG A; note that binding of USP4 to CtIP and RAD50 was not abrogated by the DNA intercalating agent ethidium bromide (EtBr), suggesting that interaction was not mediated by DNA bridging."
sparser
"To confirm complex formation of endogenous components, proximity ligation assay (PLA) was performed using the breast cancer cell line MDA-231-BM, known to produce high levels of hyaluronan. xref Using antibodies against HAS2 and USP4 or USP17, reactive signals in the form of red dots indicating interactions between endogenous HAS2 and USP17, and HAS2 and USP4, were observed; only a low background level of dots was seen when each antibody was used alone, or in the fixation control without antibody ( xref )."
Ubiquitin binds peptidase activity and USP4. 1 / 1
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1
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
USP4 affects insulin receptors
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4
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USP4 affects activity
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USP4 affects Proteasome
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2
"By screening a library of ubiquitin proteases, we further identify the Ubiquitin-Specific Protease 4 (USP4) as an enzyme that removes ubiquitin from PDK1 in vivo and in vitro and co-localizes with PDK1 at the plasma membrane when the two proteins are overexpressed, indicating direct deubiquitination."
USP4 affects IKK_complex
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Modified USP4 leads to the dephosphorylation of IKK_complex. 2 / 2
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USP4 leads to the dephosphorylation of IKK_complex. 2 / 2
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USP4 affects EV71
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USP4 inhibits EV71.
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USP4 decreases the amount of EV71.
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1
reach
"In order to confirm the relationship between USP4 and EV71 infection, USP4 was transfected into EV71 infected RD cells and it was found that the overexpression of USP4 decreased the mRNA and protein expression of EV71 and VP1, inhibiting the replication of EV71, while also reducing RD cell apoptosis."
USP15 affects BRAP
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4
UBL affects DUSP
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4
Proteasome affects USP4
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2
reach
"Our results indicate that gastrodin promotes the phosphorylation of GATA1 via the PI3K/AKT pathway, enhances the transcriptional activity of GATA1, and then increases the expression level of USP4, thereby reducing the ubiquitination and degradation of insulin receptors and ultimately improving insulin resistance."
reach
"Furthermore, gastrodin significantly upregulated the expression of USP4 and increased the binding of USP4 to the insulin receptor.Considering the importance of the PI3K-Akt pathway in the pathogenesis of T2DM, we detected the effects of gastrodin on the PI3K-Akt pathway to further explore the underlying mechanism of gastrodin in ameliorating insulin resistance."
E3_Ub_ligase affects USP4
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BRAP affects USP15
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USP4 affects signal transduction
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USP4 affects degradation
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3
USP4 affects Ubiquitination
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3
sparser
"To confirm complex formation of endogenous components, proximity ligation assay (PLA) was performed using the breast cancer cell line MDA-231-BM, known to produce high levels of hyaluronan. xref Using antibodies against HAS2 and USP4 or USP17, reactive signals in the form of red dots indicating interactions between endogenous HAS2 and USP17, and HAS2 and USP4, were observed; only a low background level of dots was seen when each antibody was used alone, or in the fixation control without antibody ( xref )."
sparser
"To confirm complex formation of endogenous components, proximity ligation assay (PLA) was performed using the breast cancer cell line MDA-231-BM, known to produce high levels of hyaluronan. xref Using antibodies against HAS2 and USP4 or USP17, reactive signals in the form of red dots indicating interactions between endogenous HAS2 and USP17, and HAS2 and USP4, were observed; only a low background level of dots was seen when each antibody was used alone, or in the fixation control without antibody ( xref )."
sparser
"To confirm complex formation of endogenous components, proximity ligation assay (PLA) was performed using the breast cancer cell line MDA-231-BM, known to produce high levels of hyaluronan. xref Using antibodies against HAS2 and USP4 or USP17, reactive signals in the form of red dots indicating interactions between endogenous HAS2 and USP17, and HAS2 and USP4, were observed; only a low background level of dots was seen when each antibody was used alone, or in the fixation control without antibody ( xref )."
EV71 affects USP4
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Torcetrapib affects USP4
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Sodium arsenite affects USP4
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Short hairpin RNA affects USP4
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Methyl methanesulfonate affects USP4
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Foci affects USP4
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Benzo[a]pyrene affects USP4
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Vialinin A affects USP4
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USP4 affects localization
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USP4 bound to TP53 activates localization. 1 / 1
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1
reach
"USP4, for example, interacts directly with end resection factors CtIP and MRN [172], USP21 deubiquitinates and stabilizes BRCA2 to promote RAD51 binding and successful HR [173], and USP10 deubiquitinates and stabilizes p53 to promote its nuclear localization and apoptosis in response to DSBs [174]."
USP4 activates localization. 1 / 1
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USP4 affects hyaluronan
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USP4 affects foci
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2
reach
"The Adenosine A2 receptor is deubiquitinated by USP4; the ubiquitination status and trafficking of the EGFR growth factor receptor is regulated by the USP8 and STAM complex; the beta2-AR undergoes increased agonist stimulated ubiquitination, lysosomal trafficking, and degradation after knockdown of USPs 20 and 33."
reach
"In the present study, we found that the USP4 and beta-catenin axis was involved in metastatic potential through USP4 mediated stabilization of beta-catenin and that knockdown of USP4 and beta-catenin suppressed the metastatic potential including clonogenicity, migration, and invasion, and induced MET by downregulating ZEB1 expression."
USP4 affects UBL domain
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2
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
reach
"In this context, to achieve a certain biological outcome, the same DUB can regulate the pathway at different levels (e.g., USP15 and USP4 promote signaling by targeting the TGF-beta receptor and the effector SMADs) or multiple DUBs can act on the same target (e.g., USP15 and OTUB1 promote signaling through stabilizing R-SMADs)."
reach
"Both USP15 and USP4 stimulate TGF-beta signaling by deubiquitylating and stabilizing two key signaling molecules in this pathway, TGF-beta receptor I (TbetaRI) and R-SMADs, suggesting that these two closely related DUBs act in concert to modulate central signaling processes that are involved in oncogenesis and innate immunity."
USP4 affects TA
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2
sparser
"In particular, the genes like TaMKP1, TaECS, TaGGT, TaYqgF, TaGBF1, TaCML11, TaEXPB23, TaWRKY2, SR3WRSI5, TaMYB19, TdSHN1, TaTPS1, TaNHX2, TaWNK1, TaRSL4 A-homeolog, TaAPX7, TaAQP8, TaBIERF3, TaAOX1a, TaPP2C1, PI4K, TaAPXb, TaHsfA7, TaCIPK29, TaMYB30-B, TaHAP2E, TaWRKY19, TaC3H50, TaCLC-1, TaSAMDC, TaSRG, TaSnRK2.7, TaERF4, TaCRT1, TaSOS1, TaHBP1b, TaTPC1, TaSOS2, TaST, TaPUB15, TaAIDFa, TaSIP, TaDi19A, TaRab7, TaCML8, TaMYB3R1, TaSNAC2, TaBADH1, TaMyb3R-2, TaSnRK2.4, TaP5CR, TaCBSX4, TaCIPK14, W69, TaWRKY44, TaMAPK44, TaSDIR1, TaSOD2, TNHXS1, TaNAC, TaRINO1, TaPEX11-1, TaCIPK15, TaSnRK2.8, TaNAC2, TaCML5, TaPOP5, TaSC, TaglyII, TaNAC5, Ta-sro1, TaACA6, TabZIP71, TaSK5, TaNAC47, TaWRKY93, TaABL1, TaSRZ1, Tamyb4, TaMGD, TaNOA1, TaRacB, TaMSRMK3, TaiSAP8, TaDSM1, TaSKIPa, TaAOC1, TaSRWD3, TaCIPK25, TaMIOX, TVP1, TaERF1, Ta-UnP, TaSRWD2, TaABP, TaMSRB, TaWRKY79, TaNIP and TaMAPK1 were expressed in most of the tissues and in all ten developmental stages, as well (Figs. xref , xref )."
| PMC
sparser
"Ten genes viz., TaAPX7, TaCam1-1, TaSST, TaSOD2, TaPEX11-1, TaGly-I, TaMIOX, Ta-UnP, TaNIP and TaERF4 were upregulated, whereas the expression of two genes (viz., TaCRT1 and TaTZF1 ) were both down-regulated in shoot tissues during the tillering stage under salt stress (Fig. xref )."
| PMC
sparser
"To investigate if Snail1 can indeed bind and regulate the USP4 promoter, luciferase reporter constructs were generated in which luciferase expression was controlled by the wild type USP4 promoter or promoters with the E-box sequence mutations shown in xref and co-transfected with Snail1 into HEK293 cells."
reach
"The Cell Counting Kit-8 (CCK-8) assay results showed that the proliferation of hypertrophic scar fibroblasts transfected with siUSP4 was significantly inhibited on the fifth and seventh day, indicating that down-regulation of USP4 could suppress the proliferation of hypertrophic scar fibroblasts."
USP4 affects NF-kappaB luciferase
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2
sparser
"We carried out ubiquitination assays by co-transfecting HEK293T cells with a Myc-HDAC2 expression plasmid plus His and Flag empty vectors, His-Ubiquitin and Flag empty vectors, His-Ubiquitin, Flag-USP4 and Flag empty vectors,or His-Ubiquitin, Flag-USP4 and Flag-TRPS1 vectors and compared them with cells transfected with Myc plus His vector and Flag-empty vectors."
USP4 affects ERK1/2-MAPK
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2
USP4 affects DSB repair
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2
USP4 affects DNA Breaks, Double-Stranded
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2
USP4 affects CrkII
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2
USP4 affects Cell Survival
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1
1
USP4 affects Carcinogenesis
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1
USP4 activates Carcinogenesis. 1 / 2
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1
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
USP4 affects Angiotensin-2
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2
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
UBL domain affects USP4
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2
reach
"USP4, for example, interacts directly with end resection factors CtIP and MRN [172], USP21 deubiquitinates and stabilizes BRCA2 to promote RAD51 binding and successful HR [173], and USP10 deubiquitinates and stabilizes p53 to promote its nuclear localization and apoptosis in response to DSBs [174]."
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
TA affects USP4
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2
sparser
"In particular, the genes like TaMKP1, TaECS, TaGGT, TaYqgF, TaGBF1, TaCML11, TaEXPB23, TaWRKY2, SR3WRSI5, TaMYB19, TdSHN1, TaTPS1, TaNHX2, TaWNK1, TaRSL4 A-homeolog, TaAPX7, TaAQP8, TaBIERF3, TaAOX1a, TaPP2C1, PI4K, TaAPXb, TaHsfA7, TaCIPK29, TaMYB30-B, TaHAP2E, TaWRKY19, TaC3H50, TaCLC-1, TaSAMDC, TaSRG, TaSnRK2.7, TaERF4, TaCRT1, TaSOS1, TaHBP1b, TaTPC1, TaSOS2, TaST, TaPUB15, TaAIDFa, TaSIP, TaDi19A, TaRab7, TaCML8, TaMYB3R1, TaSNAC2, TaBADH1, TaMyb3R-2, TaSnRK2.4, TaP5CR, TaCBSX4, TaCIPK14, W69, TaWRKY44, TaMAPK44, TaSDIR1, TaSOD2, TNHXS1, TaNAC, TaRINO1, TaPEX11-1, TaCIPK15, TaSnRK2.8, TaNAC2, TaCML5, TaPOP5, TaSC, TaglyII, TaNAC5, Ta-sro1, TaACA6, TabZIP71, TaSK5, TaNAC47, TaWRKY93, TaABL1, TaSRZ1, Tamyb4, TaMGD, TaNOA1, TaRacB, TaMSRMK3, TaiSAP8, TaDSM1, TaSKIPa, TaAOC1, TaSRWD3, TaCIPK25, TaMIOX, TVP1, TaERF1, Ta-UnP, TaSRWD2, TaABP, TaMSRB, TaWRKY79, TaNIP and TaMAPK1 were expressed in most of the tissues and in all ten developmental stages, as well (Figs. xref , xref )."
| PMC
sparser
"Ten genes viz., TaAPX7, TaCam1-1, TaSST, TaSOD2, TaPEX11-1, TaGly-I, TaMIOX, Ta-UnP, TaNIP and TaERF4 were upregulated, whereas the expression of two genes (viz., TaCRT1 and TaTZF1 ) were both down-regulated in shoot tissues during the tillering stage under salt stress (Fig. xref )."
| PMC
sparser
"To investigate if Snail1 can indeed bind and regulate the USP4 promoter, luciferase reporter constructs were generated in which luciferase expression was controlled by the wild type USP4 promoter or promoters with the E-box sequence mutations shown in xref and co-transfected with Snail1 into HEK293 cells."
Rho52 affects USP4
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2
RBBP8 affects foci
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2
GC affects USP4
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2
sparser
"We carried out ubiquitination assays by co-transfecting HEK293T cells with a Myc-HDAC2 expression plasmid plus His and Flag empty vectors, His-Ubiquitin and Flag empty vectors, His-Ubiquitin, Flag-USP4 and Flag empty vectors,or His-Ubiquitin, Flag-USP4 and Flag-TRPS1 vectors and compared them with cells transfected with Myc plus His vector and Flag-empty vectors."
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
sparser
"cDNAs encoding Myc-tagged forms of USP4, USP11, Ataxin-3 and A20 were gifts from Dr P.J. Lehner (University of Cambridge, UK) (51), Dr J. Yang (Baylor College of Medicine, Houston, USA) (52), Dr E.A. Fon (Montreal Neurological Institute, Canada) (23) and Dr K. Li (University of Texas Medical Branch, Galveston, USA) (53), respectively."
Vinclozolin affects USP4
1
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Valproic acid affects USP4
1
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Type I receptor affects USP4
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1
Temozolomide affects USP4
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1
Temozolomide activates USP4. 1 / 1
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1
ShRNA lentiviral transduction particles affects USP4
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1
Selenium atom affects USP4
1
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Resveratrol affects USP4
1
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Pirinixic acid affects USP4
1
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Peptidase activity affects Ubiquitin
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1
Pentachlorophenol affects USP4
1
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Paracetamol affects USP4
1
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MiR-553 affects USP4
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1
MiR-148a affects USP4
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1
Lipopolysaccharide affects USP4
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1
Lipopolysaccharide decreases the amount of USP4. 1 / 1
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1
Isopeptidase activity affects USP4
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1
Isopeptidase activity binds USP4. 1 / 1
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1
Insulin receptors affects USP4
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1
Importin affects USP4
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1
Hsa_circ_0001326 affects USP4
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1
Gene product affects USP4
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1
Endonuclease CtIP affects USP4
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1
Coumestrol affects USP4
1
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CooH affects adenosine receptor
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1
CircBMPR2 affects USP4
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1
Catalytic affects USP4
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1
Catalytic domain turnover affects USP4
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1
Bisphenol A affects USP4
1
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Adenosine receptor affects cooH
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1
VLX1570 affects USP4
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1
UnpDelta1-168H880A affects USP4
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1
Ubl domain affects USP4
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1
Ubiquitin affects peptidase activity
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1
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
USP4 affects verify above results
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1
USP4 affects type I receptor
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1
USP4 affects type 2 inflammatory cytokine gene
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1
USP4 affects subsequent immune responses
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1
USP4 affects siUSP4-2
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1
USP4 affects several inflammatory cytokines T cells
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1
USP4 affects reactive oxygen species
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1
USP4 activates reactive oxygen species. 1 / 1
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1
USP4 affects protein stability ELAVL1
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1
USP4 affects polyubiquitin chain
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1
USP4 affects phosphorylation p-S6
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1
USP4 affects peptidase activity
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1
USP4 affects pathway
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1
USP4 affects pathological cardiac hypertrophy
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1
USP4 affects p53 transcriptional
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1
USP4 affects p-CrkII
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1
USP4 affects osteoblast differentiation
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1
USP4 activates osteoblast differentiation. 1 / 1
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1
USP4 affects oncogene-induced primary cell transformation
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1
eidos
"Indeed , it has been suggested that Usp4 is a potential oncogene because it inhibits p53 activity , p53-associated apoptosis and cell-cycle checkpoints ; depletion of USP4 promotes cell senescence ; loss of USP4 inhibits oncogene-induced primary cell transformation , and finally USP4 is over-expressed in a subset of human cancers [ 86 ] ."
USP4 affects nuclear factor-kappaB
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1
USP4 affects nitric oxide
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1
USP4 decreases the amount of nitric oxide. 1 / 1
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1
USP4 affects necrotic cell death
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1
USP4 inhibits necrotic cell death. 1 / 1
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1
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1
USP4 activates movement of cell or subcellular component. 1 / 1
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1
USP4 affects migration
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1
USP4 affects j different USP4 mutants USP4-WT S445A S445D mTPRC1 activation Considering Rheb ubiquitination
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1
USP4 affects isopeptidase activity
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1
Isopeptidase activity binds USP4. 1 / 1
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1
USP4 affects interleukin-4 secretion
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1
USP4 activates interleukin-4 secretion. 1 / 1
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1
eidos
"In the study of rheumatic heart disease , Guo et al. revealed that USP4 positively modulated IL-4 secretion by T helper type 2 cells through deubiquitinating and stabilizing IRF4 which is usually the reason of allergic immune responses diseases , such as rheumatic heart disease [ 62 ] ."
USP4 affects inflammatory responses
|
1
USP4 affects inflammation liver R injury
|
1
USP4 affects importin
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1
USP4 affects gene product
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1
USP4 affects formation
|
1
USP4 affects fibrosis progress autoimmune hepatitis
|
1
USP4 affects endonuclease CtIP
|
1
USP4 affects dissociation TSC
|
1
USP4 affects deubiquitination translocation SMAD4
|
1
USP4 affects defense response to virus
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1
USP4 activates defense response to virus. 1 / 1
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1
USP4 affects de-ubiquitination recycling Prp3
|
1
USP4 affects content PRL-3
|
1
USP4 affects cleaved-PARP
|
1
USP4 affects cell migration invasion breast cancer
|
1
USP4 affects cell death
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1
USP4 activates cell death. 1 / 1
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1
USP4 affects catalytic activity
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1
USP4 affects breast cell invasion
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1
USP4 affects breast cancer cell migration
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1
USP4 affects beta-catenin signalling inducing Runx2
|
1
USP4 affects antiviral immune response
|
1
USP4 affects activation polyubiquitinated TAK1
|
1
USP4 affects activation NF-kappaB
|
1
USP4 affects activation NF-kappaB AP-1 transcription
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1
USP4 affects Ubiquitin, and peptidase activity
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1
USP4 affects Tumor Growth
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1
USP4 affects Transferase
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1
USP4 binds Transferase. 1 / 1
|
1
USP4 affects Th17
|
1
USP4 affects TNF-alpha-induced IkappaB-alpha
|
1
reach
"Studies focused on the other USPs demonstrated that USP14 overexpression regulates IkappaB polyubiquitination to stimulate its degradation 26, and ectopic expression of USP4 inhibits the TRAF2- and TRAF6 stimulated NF-kappaB reporter gene and negatively regulates the TNF-alpha-induced IkappaB-alpha degradation and NF-kappaB activation 27."
reach
"Considering the function of the regulatory axis of TRPS1, USP4, and HDAC2 on transcriptional regulation, we further confirmed that silencing USP4 led to consistent up-regulation of AES, CASP7, IFT27, PERP, SHISA2, TPM4, and ZW10, and additional overexpression of HDAC2 restored the expression levels of these genes (Fig. 5e, f)."
USP4 affects TH2-related cytokines
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1
USP4 affects TGFbetaRI
|
1
USP4 affects TGFbeta receptor
|
1
USP4 affects TGF-betaR1
|
1
USP4 affects TGF-beta-induced EMT
|
1
USP4 affects RORgammat
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1
USP4 affects Phosphatase
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1
USP4 increases the amount of Phosphatase. 1 / 1
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1
USP4 affects PI3K Akt
|
1
USP4 affects PI3K Akt pathway
|
1
USP4 affects PC
|
1
USP4 affects NFAT-mediated Il4 promoter activity
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1
USP4 affects N-end rule substrate Tyr-beta-galactosidase
|
1
USP4 affects MTOR
|
1
reach
"Conversely, whereas USP4 and USP15 target p53 inhibiting ligases ARF-BP1 [XREF_BIBR] and MDM2 [XREF_BIBR], respectively, USP11 stabilizes p53 [XREF_BIBR] as well as several other tumor suppressors including PML [XREF_BIBR], BRCA2 [XREF_BIBR] and Mre11 complex members MRE11 & RAD50 [XREF_BIBR]."
USP4 affects Lys63
|
1
reach
"In view of the data presented here and previous reports, we propose a working model (XREF_FIG), in which that TNFalpha rapidly induces Lys63 linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63 linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFalpha induced Lys63 linked TAK1 polyubiquitination and TAK1 mediated IKK and NF-kappaB activation."
USP4 affects LvBr4
|
1
USP4 affects LC3II
|
1
USP4 affects KSPRK
|
1
USP4 affects K48-linked ubiquitination RIG-I
|
1
USP4 affects Ischemic Stroke
|
1
USP4 activates Ischemic Stroke. 1 / 1
|
1
USP4 affects IGF-I-Myr-AKT-induced migration MDA-MB-231 breast cancer cells
|
1
USP4 affects Hypertrophy
|
1
USP4 inhibits Hypertrophy. 1 / 1
|
1
USP4 affects Histone_H1
|
1
USP4 leads to the phosphorylation of Histone_H1. 1 / 1
|
1
USP4 affects Glioblastoma multiforme GBM
|
1
USP4 affects EMT process liver cancer
|
1
USP4 affects DUSP domain
|
1
USP4 affects DSB Repair
|
1
USP4 affects CD44s protein
|
1
USP4 affects CCCP-induced clustering ubiquitin mitochondria
|
1
reach
"Indeed, it has been suggested that Usp4 is a potential oncogene because it inhibits p53 activity, p53-associated apoptosis and cell-cycle checkpoints; depletion of USP4 promotes cell senescence; loss of USP4 inhibits oncogene-induced primary cell transformation, and finally USP4 is over-expressed in a subset of human cancers [86]."
USP4 affects 293T
|
1
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
sparser
"In the absence of USP4 and USP15 ubiquitin-bound structures, the molecular bases for these differences are difficult to rationalize at present, as multiple factors will contribute, such as the release of bound water molecules, conformational changes associated with the binding events, and differences in interacting residues."
USP15 affects E3_Ub_ligase
|
1
UBL domain affects CTNNB1
|
1
Transferase affects USP4
|
1
USP4 binds Transferase. 1 / 1
|
1
TGFbetaRI affects USP4
|
1
Snail1 affects USP4
|
1
Plant Extracts affects USP4
1
|
Particulate Matter affects USP4
1
|
PR-619 affects USP4
|
1
MYC affects BRAP
|
1
METTL3 overexpression affects USP4
|
1
MAP3K7 affects Lys63
|
1
reach
"In view of the data presented here and previous reports, we propose a working model (XREF_FIG), in which that TNFalpha rapidly induces Lys63 linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63 linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFalpha induced Lys63 linked TAK1 polyubiquitination and TAK1 mediated IKK and NF-kappaB activation."
Lys63 affects USP4
|
1
reach
"In view of the data presented here and previous reports, we propose a working model (XREF_FIG), in which that TNFalpha rapidly induces Lys63 linked TAK1 polyubiquitnation and binding of USP4 to TAK1, Lys63 linked TAK1 would be rapidly deubiquitinated by USP4 to attenuate the magnitude of TNFalpha induced Lys63 linked TAK1 polyubiquitination and TAK1 mediated IKK and NF-kappaB activation."
E3_Ub_ligase affects bznB
|
1
DUSP-Ubl domain affects USP4
|
1
DUSP domain affects USP4
|
1
CTNNB1 affects 293T
|
1
Brodifacoum affects USP4
1
|
BRAP affects USPs
|
1
Antirheumatic Agents affects USP4
1
|
1
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293T affects USP4
|
1
17alpha-ethynylestradiol affects USP4
1
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4,4'-diaminodiphenylmethane affects USP4
1
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3,4,3',4'-tetrachlorobiphenyl affects USP4
1
|
1
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