IndraLab

Statements

USP4 binds DUSP. 6 / 6
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"We were successful in producing the USP4-DUSP, USP11-DUSP, USP15-DUSP, USP20-DUSP2 and USP33-DUSP2 recombinant proteins in Escherichia coli and purified them to near homogeneity from the soluble fract[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34666042
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"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
34666042
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"For USP4-DUSP, none of the 11 UbVs selected for binding exhibited non-specific binding to SAV but nine UbVs (4D."
34666042
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"1 and 2) were absolutely specific for USP4-DUSP."
34666042
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"1 bound to its target USP4-DUSP with an affinity in the high nanomolar range (K D ∼ 500 nM)."
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"Another protein, NBS1, which is a part of the MRN complex, interacts with the DUSP domain of USP4 and recruits the enzyme to DNA damage sites where it colocalizes with CtIP ( xref )."
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