IndraLab

Statements

USP4 binds TA. 52 / 52
| 52
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"These results indicate that Ta-UnP was mainly localized in the cell membrane."
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"A wheat full-length cDNA sequence ( Ta-UnP ) with a complete coding region was cloned via gene splicing."
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"The Cl − levels increased by 3.61-fold in the CK plants, by 3.39-fold in the Ta-UnP overexpressing rice and by 4.60-fold in RNAi lines compared with the levels before salt treatment ( Fig. 3 F)."
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"Detection of seed germination: The transgenic plants and WT control plants presented similar seed germination on the 0 mM NaCl MS plate, whereas the germination of Ta-UnP homozygous seeds was much bet[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Following NaCl treatment, the Na + levels increased by 1.77-fold in the CK plants, by 1.34-fold in the Ta-UnP overexpressing rice and by 2.99-fold in RNAi lines compared with the levels before salt tr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Although both lines showed some increase in Na + and Cl − content after salt treatment, the content of Na + and Cl − in the Ta-UnP overexpressing rice plants was significantly lower than that in the C[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In particular, the genes like TaMKP1, TaECS, TaGGT, TaYqgF, TaGBF1, TaCML11, TaEXPB23, TaWRKY2, SR3WRSI5, TaMYB19, TdSHN1, TaTPS1, TaNHX2, TaWNK1, TaRSL4 A-homeolog, TaAPX7, TaAQP8, TaBIERF3, TaAOX1a, TaPP2C1, PI4K, TaAPXb, TaHsfA7, TaCIPK29, TaMYB30-B, TaHAP2E, TaWRKY19, TaC3H50, TaCLC-1, TaSAMDC, TaSRG, TaSnRK2.7, TaERF4, TaCRT1, TaSOS1, TaHBP1b, TaTPC1, TaSOS2, TaST, TaPUB15, TaAIDFa, TaSIP, TaDi19A, TaRab7, TaCML8, TaMYB3R1, TaSNAC2, TaBADH1, TaMyb3R-2, TaSnRK2.4, TaP5CR, TaCBSX4, TaCIPK14, W69, TaWRKY44, TaMAPK44, TaSDIR1, TaSOD2, TNHXS1, TaNAC, TaRINO1, TaPEX11-1, TaCIPK15, TaSnRK2.8, TaNAC2, TaCML5, TaPOP5, TaSC, TaglyII, TaNAC5, Ta-sro1, TaACA6, TabZIP71, TaSK5, TaNAC47, TaWRKY93, TaABL1, TaSRZ1, Tamyb4, TaMGD, TaNOA1, TaRacB, TaMSRMK3, TaiSAP8, TaDSM1, TaSKIPa, TaAOC1, TaSRWD3, TaCIPK25, TaMIOX, TVP1, TaERF1, Ta-UnP, TaSRWD2, TaABP, TaMSRB, TaWRKY79, TaNIP and TaMAPK1 were expressed in most of the tissues and in all ten developmental stages, as well (Figs.  xref , xref )."
| PMC
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"Ta-UnP was cloned by taking cDNA as the template, and the sequence was deposited in GenBank (accession No."
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"The cloned Ta-UnP sequence was subcloned into the expression vector pCAMBIA2300-35S-GFP-OCS (abbrev."
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"The cloned Ta-UnP cDNA was subcloned into the expression vector pCAMBIA1300."
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"In the leaves of the non-salt-tolerant RH8706-34 plants, salt, PEG, or ABA treatment only slightly changed the Ta-UnP transcript levels ( Fig. 1 B)."
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"The expression of Ta-UnP in the roots of RH8706-49 did not follow the same trend as that in the leaves."
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"Salt treatment induced the expression of Ta-UnP in roots at most of the time points of treatment, and the highest expression level (9.77-fold that of the control sample) was observed 1 h after salt tr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Under ABA stress, the expression of Ta-UnP increased at most of the time points, and the highest level (7.79-fold that of the control) was reached after 12 h of treatment."
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"For PEG stress, the expression of Ta-UnP fluctuated, and its highest level (5.56-fold that of the control) was reached after 6 h of treatment."
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"Similar to that in the leaves, the expression of Ta-UnP in the roots of RH8706–34 under salt treatment did not significantly change ( Fig. 1 C)."
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"The amplified Ta-UnP was cloned into the pTCK303 overexpression vector for transformation into rice lines."
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"An RNA interference (RNAi) knockdown construct was prepared using the Ta-UnP homologous sequence Os03g0407100 in rice and then cloned into the expression vector pTCK303 as a hairpin construct."
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"It was a novel gene of unknown function, and Ta-UnP is 70% homologous to rice Os03g0407100 protein."
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"It was named Ta-UnP ( Triticum aestivum unknown protein) (GenBank accession No."
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"In tobacco leaves infiltrated with Agrobacterium harboring a Ta-UnP -GFP fusion construct, microscopic results revealed that fluorescence was mainly localized in the cell membrane ( Fig. 2 A–C)."
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"Based on the qPCR results, Ta-UnP was induced in leaves of salt-tolerant wheat cultivar with the highest Ta-UnP transcript level (7.49-fold that of the untreated control) detected after 72 h of treatm[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Following treatment with 180 mM NaCl, the levels of proline and soluble sugars were higher in the Ta-UnP transgenic plants than those in the WT plants."
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"In the salt-treated Ta-UnP transgenic plants, the levels of these three compounds increased by 1.56-, 1.26-, and 0.91-fold, which were all significantly higher compared with those in the WT plants."
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"After 3 d of treatment with 150 mM NaCl, the Ta-UnP transgenic plants produced roots that were 1.66-fold longer than the WT plants ( Fig. 3 C and D)."
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"The overexpression of Ta-UnP significantly enhanced the salt tolerance of transgenic Arabidopsis ( Fig. 3 E)."
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"After 10 d of treatment with 170 mM NaCl Ta-UnP overexpressing rice, L2, L3, and L6 grew well, whereas the control plants wilted."
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"The detected physiological indices were present at significantly different levels in the WT and Ta-UnP transgenic plants."
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"The expression of Ta-UnP was induced by salt in salt-tolerant mutant RH8706-49, which suggests that Ta-UnP may participate in pathways responding to salt stress and thus confer salt tolerance to the i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The expression of Ta-UnP in RH8706-49 was highly induced after 50 μM ABA treatment, which suggests that Ta-UnP may participate in the salt stress response mechanism through the ABA signal transduction[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The expression of Ta-UnP was also significantly induced after PEG stress, thereby indicating that Ta-UnP may close the stomata through the ABA signal transduction pathway to reduce water loss and thus[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The following analysis of salt tolerance in Ta-UnP overexpressing Arabidopsis plants demonstrated that transgenic Arabidopsis exhibited better performance in terms of germination rate, root length, pl[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"This finding confirms the effectiveness of Ta-UnP in enhancing the salt tolerance of transgenic plants."
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"Rice was selected to further explore the salt tolerance mechanism of Ta-UnP because of the closer genetic relationship of rice with wheat than with Arabidopsis ."
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"In this research, Ta-UnP functions are further discussed based on the interference homologous gene (Os03g0407100) of Ta-UnP and overexpressed Ta-UnP in rice plants."
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"In this experiment, Ta-UnP overexpression can enhance the salt tolerance of transgenic rice lines."
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"RNAi knockdown transgenic lines become susceptible to salt stress because RNAi technology successfully inhibits the expression of Ta-UnP homologous genes in rice lines, which confirms that Ta-UnP can [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To explore the salt tolerance mechanism of Ta-UnP , we measured the physiological indices of transgenic plants."
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"After salt stress, transgenic plants accumulated abundant proline and soluble sugar, thereby indicating that Ta-UnP can facilitate the accumulation of osmotic substances, such as proline and soluble s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"After salt treatment, the MDA content in Ta-UnP -overexpressed Arabidopsis was significantly lower than that in the control plants, which indicates that the transgenic plants experienced less severe m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The detection result confirmed that Ta-UnP can reduce the damage caused by salt stress to the plant photosynthetic system, thereby improving its salt tolerance."
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"Detection of Na + and Cl - contents in Ta-UnP overexpressing rice plants and RNAi lines shows that Na + and Cl - contents significantly higher in RNAi lines than that in CK control, but keep low in Ta[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"The wheat gene Ta-UnP regulates the expression of P5CS, and the overexpression of Ta-UnP in rice can highly induce the expression of OsP5CS1 , resulting in increased proline content, and improving the osmotic and salt stress tolerance of rice [ xref ]."
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"This detection result further confirmed that Ta-UnP can reduce damages to plants caused by salt stress and enhance salt tolerance of transgenic plants."
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"Ten genes viz., TaAPX7, TaCam1-1, TaSST, TaSOD2, TaPEX11-1, TaGly-I, TaMIOX, Ta-UnP, TaNIP and TaERF4 were upregulated, whereas the expression of two genes (viz., TaCRT1 and TaTZF1 ) were both down-regulated in shoot tissues during the tillering stage under salt stress (Fig.  xref )."
| PMC
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"The qPCR results showed that SOS2 , SO S 3, RD29B , and P5CS were induced to a greater extent in the transgenic plants than that in the control plants, which indicates that Ta-UnP may affect their exp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Accordingly, the expression levels of Ta-UnP in the roots and leaves of RH8706-49 increased after ABA and PEG treatment."
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"Based on these results, we postulate that Ta-UnP may enhance salt tolerance via the SOS and ABA signal transduction pathways."
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"Wheat Ta-UnP , which was amplified by our laboratory for the first time, can significantly improve the salt tolerance of transgenic Arabidopsis and rice."
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"Moreover, the expression level of P5CS in Ta-UnP transgenic plants became highly induced."
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"Ta-UnP could affect the expression of P5CS gene through the ABA signaling pathway to improve the content of proline in transgenic plants to resist osmotic stress and improve the salt tolerance of plan[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Our laboratory studies have shown that wheat genes Ta-UnP , TaZNF , TaSST , TaDUF1 , and TaSP can significantly improve the salt tolerance of transgenic plants [1,44–47] ( Fig. 4 )."
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