IndraLab

Statements

USP4 binds RBBP8. 48 / 56
6 | 18 30
sparser
"USP4 interacts with the retinoblastoma binding protein 8 (CtIP, also known as RBBP8) and the MRE11RAD50-NBS1 (MRN) complex [ xref , xref ]."
30831436
sparser
"Because USP4 autodeubiquitination did not affect its levels, the next question we asked was whether USP4 autodeubiquitination could affect USP4-CtIP interaction."
26387952
sparser
"As shown in Figure 7 B, USP4 catalytic activity is essential for the USP4-CtIP interaction."
26387952
sparser
"USP4-CtIP interaction is a little increased after IR treatment, but in the USP4CA mutant cells, USP4-CtIP interaction is almost gone, even in IR-treated cells."
26387952
sparser
"This result suggests that USP4 promotes USP4-CtIP interaction specifically through its deubiquitinase activity and likely through its autodeubiquitination."
26387952
reach
"They both demonstrated that USP4 interacts with CtIP and MRN and regulates the recruitment of CtIP to DSBs."
27602047
sparser
"These are consistent with our results; CtIP N-terminal could interact with USP4, and this interaction is essential for DNA end resection."
26387952
sparser
"USP4 interacts with CtIP and MRN via the C-terminal insert domain (residues 572–773) and intact catalytic domain of USP4, respectively."
26387952
sparser
"More importantly, USP4 ubiquitination can block USP4-CtIP interaction, whereas the USP4CA mutant could not mediate its autodeubiquitination, and sustained ubiquitination of USP4 would block its intera[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26387952
sparser
"Of note, USP4 can deubiquitinate itself, and this autodeubiquitination function is crucial for the USP4-CtIP interaction, which occurs in HR ( xref ; xref )."
33681193
reach
"USP4 interacts with CtIP and regulates the recruitment of CtIP to DNA damage sites through its deubiquitylating enzyme activity."
29244158
sparser
"USP4 binds CtIP and MRN complexes to regulate DDR and thereby promotes homologous recombination [ xref ]."
39300775
sparser
"We also could not exclude other mechanisms; for example, there might be other factors that help the deubiquitinated USP4 interact with CtIP."
26387952
sparser
"Our results revealed that USP4 interacts with CtIP and USP4-CtIP interaction is important for HR."
26387952
reach
"USP4 binds CtIP and MRN complexes to regulate DDR and thereby promotes homologous recombination [42]."
39300775
sparser
"Of note, the interaction between USP4 and the DNA endonuclease CtIP (RDDP8), critical to DSB end resection in xref ; xref ), represents a postWGD neofunctionalization."
28177072
sparser
"In addition, USP4 interacts with MRN and CtIP promotes the interaction between these proteins [ xref , xref ]."
32570875
sparser
"USP4 interacts with CtIP, in addition to NBS1 as part of the MRN complex, with USP4 depletion reducing the recruitment of CtIP, RPA, and RAD51 loading to DSB sites [ xref ]."
38572758
sparser
"We set out to detangle the DUB network and distinguish redundancy that results from postduplicative conservation of interaction domains from neofunctionalization events, such as the WGD-derived USP4-CtIP interaction in DSB repair."
28177072
reach
"Deletion of USP4 insert domain (residues 572-775) abolished the binding of USP4 with CtIP."
26387952
reach
"USP4 interacts with the retinoblastoma binding protein 8 (CtIP, also known as RBBP8) and the MRE11RAD50-NBS1 (MRN) complex [XREF_BIBR, XREF_BIBR]."
30831436
reach
"As USP4 directly interacts with CtIP, we also checked CtIP foci formation in USP4 depleted cells."
26387952
reach
"These results indicate that the deubiquitylating enzyme activity of USP4 is required for its function in HR.Our results revealed that USP4 interacts with CtIP and USP4-CtIP interaction is important fo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26387952
sparser
"USP4 interacts with CtIP and regulates the recruitment of CtIP to DNA damage sites through its deubiquitylating enzyme activity ( xref )."
29244158
reach
"Here, we report that USP4 interacts with both MRN complex and CtIP, which is a positive regulator of DNA end resection, thus promoting HR.Both the C terminus and the N terminus of CtIP protein are req[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26387952
reach
"These are consistent with our results; CtIP N-terminal could interact with USP4, and this interaction is essential for DNA end resection."
26387952
reach
"USP4 interacts with CtIP and MRN via the C-terminal insert domain (residues 572-773) and intact catalytic domain of USP4, respectively."
26387952
reach
"Wealso report that USP4 interacts with CtIP and the MRE11-RAD50-NBS1 (MRN) complex and is required for CtIP recruitment to DNA damage sites."
26455393
reach
"Additionally, we establish that USP4 interacts with CtIP and the MRN complex via its C-terminal insert domain and that these interactions are subject to a USP4 auto-regulatory deubiquitylation mechanism."
26455393
reach
"In light of the above findings, we tested whether USP4 might physically interact with CtIP and/or MRN."
26455393
sparser
"Notably, this USP4-mediated de-ubiquitination of BRCA1 is CtIP independent, indicating that USP4-BRCA1 axis is a distinct pathway other than USP4-CtIP in regulating HR."
38734703
sparser
"They both demonstrated that USP4 interacts with CtIP and MRN and regulates the recruitment of CtIP to DSBs."
27602047
reach
"We and others report that USP4 interacts with CtIP and the MRE11-RAD50-NBS1 (MRN) complex and is required for CtIP recruitment to DNA damage sites and DNA end resection."
27940552
sparser
"As shown in Figure 1 B, endogenous USP4 and CtIP interact with each other in cells."
26387952
reach
"These papers have reported that USP4 interacts with MRN complex and CtIP dependently on its catalytic activity."
28764946
sparser
"We did the coIP experiment after DNase treatment; this result indicated that CtIP-USP4 interaction was not bridged by DNA."
26387952
sparser
"To identify the regions of USP4 that are responsible for the USP4-CtIP interaction, we generated deletion mutants of USP4 ( Figure 1 C)."
26387952
sparser
"Deletion of USP4 insert domain (residues 572–775) abolished the binding of USP4 with CtIP."
26387952
reach
"USP4, for example, interacts directly with end resection factors CtIP and MRN [172], USP21 deubiquitinates and stabilizes BRCA2 to promote RAD51 binding and successful HR [173], and USP10 deubiquitinates and stabilizes p53 to promote its nuclear localization and apoptosis in response to DSBs [174]."
32708614
reach
"Previous study showed that CtIP interacts with the MRE11-RAD50-NBS1 (MRN) complex and USP4 which is required for CtIP recruitment to DNA damage sites."
27940552
sparser
"As USP4 directly interacts with CtIP, we also checked CtIP foci formation in USP4-depleted cells."
26387952
sparser
"Wijnhoven et al. revealed that the human deubiquitylase USP4 promotes homologous recombination, DNA end-resection, and CtIP recruitment and that USP4 interacts with CtIP and MRN."
26455393
sparser
"Because it was reported that USP4 interacts with CtIP but does not affect the latter’s ubiquitination level xref , we chose USP52 as the candidate DUB for CtIP."
33097710
sparser
"Because USP4 interacts with the N-terminal of CtIP through its insert domain, CtIP N-terminal is essential for its foci formation and depletion of USP4 decreased CtIP foci formation."
26387952
sparser
"We hypothesized that USP4-CtIP interaction is required for CtIP recruitment to the DNA damage sites."
26387952
reach
"During the course of our studies, we observed that mutating theUSP4 catalytic cysteine to alanine (C311A) to render USP4 enzymatically inactive (" catalytic-dead " [CD]), almost totally abrogated its interactions with CtIP and MRN (XREF_FIG A; note that binding of USP4 to CtIP and RAD50 was not abrogated by the DNA intercalating agent ethidium bromide (EtBr), suggesting that interaction was not mediated by DNA bridging."
26455393
sparser
"USP4 interacts with both CtIP and the MRN complex via a specific conserved region on USP4 and the catalytic domain of USP4, respectively, to promote the recruitment of the DNA end-resection factor CtIP to dsDNA break sites [ xref ]."
29641431
sparser
"These results indicate that the USP4-CtIP interaction is required for its function in HR."
26387952