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USP4 binds Flag. 14 / 14
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"Based on in vitro and in vivo experiments, we confirmed that exosomes derived from USP4‐overexpressing BMSCs (MEXOs [Flag‐USP4]) successfully deliver functional Flag‐USP4 protein into target cells, increase total USP4 levels and facilitate its interaction with endogenous ARNTL (Figure S xref )."
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"Flag-USP4 and Myc-PKM2 were co-transfected into cells, and then either immunoprecipitated with Myc and blotted with Flag, or immunoprecipitated with Flag and blotted with Myc."
37624791
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"RE: Our Co-IP assay results showed that Flag-USP4 and Myc-PKM2 co-immunoprecipitated with each other."
37624791
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"We carried out ubiquitination assays by co-transfecting HEK293T cells with a Myc-HDAC2 expression plasmid plus His and Flag empty vectors, His-Ubiquitin and Flag empty vectors, His-Ubiquitin, Flag-USP4 and Flag empty vectors,or His-Ubiquitin, Flag-USP4 and Flag-TRPS1 vectors and compared them with cells transfected with Myc plus His vector and Flag-empty vectors."
30071870
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"The result showed that BRCA1 were pulled down by Flag-USP4 from 293 T cells expressing either BRCA1 alone, or BRCA1 plus BARD1."
38734703
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"Notably, BRCA1 C61G was also pulled down by Flag-USP4 (Fig. xref )."
38734703
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"For analysis of the ubiquitination of RIP1, HEK293 cells were transfected with Myc-RIP1, HA-Ub (WT) or HA-Ub mutants and Flag-USP4, and then whole-cell extracts were immunoprecipitated with anti-Myc a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
23313255
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"We found that the anti-Myc antibody co-precipitated Flag-USP4, whereas the control serum did not ( xref )."
37624791
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"To further validate this point, we co-transfected Flag-USP4, Myc-HDAC2, and Flag-TRPS1 in HEK293T cells and performed Co-IP using anti-Myc antibody and found that TRPS1, USP4, and HDAC2 were co-purified as a complex (Fig. xref )."
30071870
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"This interaction was further corroborated in HEK 293T cells using epitope‐tagged proteins, where Flag‐USP4 co‐precipitated with Myc‐ARNTL and vice versa (Figure  xref )."
41482632
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"293 T cells were transfected with Flag-USP4, together with either BARD1 alone, BRCA1 alone, BARD1 plus BRCA1, or BARD1 plus BRCA1 C61G, a mutant of BRCA1 that does not interact with BARD1, and Flag IPs were performed."
38734703
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"Similarly, BARD1 was pulled down by Flag-USP4 from cells expressing BARD1 alone, or BARD1 plus either BRCA1 Wt or C61G (Fig. xref )."
38734703
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"Flag-USP4 and Myc-RIP1 were cotransfected into HEK293 cells, 24 h after transfection, immunoprecipitation experiments were performed with Flag or Myc antibodies."
23313255
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"As shown in Fig. 3 C, Myc-RIP1 was coprecipitated with Flag-USP4 using anti-Flag antibody and vice versa."
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