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USP4 binds RHEB. 11 / 15
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"Interestingly, our data showed that USP4-S445A barely bound to Rheb (Fig.  xref )."
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"This finding was further validated in TDSCs, wherein MA significantly inhibited the interaction between USP4 and Rheb mediated by IL‐1β, in the presence of MG132 (Figure 5D)."
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"Furthermore, interaction of USP4 and Rheb was examined by immunoprecipitation assay (Figure  xref )."
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"Since our data showed that Rheb ubiquitination was inhibited by EGF treatment (Fig.  xref ), we examined whether the interaction between USP4 and Rheb was affected by EGF treatment and found that EGF treatment promoted their interaction at both endogenous and exogenous level (Fig.  xref and Supplementary information, Fig.  xref )."
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"Further investigation found that MA significantly inhibited the interaction between Rheb and USP4 in HEK293T cells (Figure  xref )."
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"This finding was further validated in TDSCs, wherein MA significantly inhibited the interaction between USP4 and Rheb mediated by IL‐1β, in the presence of MG132 (Figure  xref )."
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"To examine whether the phosphorylation of USP4 at Ser445 affects the interaction between USP4 and Rheb, we generated a USP4 dephospho-mimetic mutant (USP4-S445A) and a phospho-mimetic mutant (USP4-S445D)."
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"However, our data showed that only USP4 interacted with Rheb (Supplementary information, Fig. S xref )."
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"Moreover, AKT inhibitor (MK2206) disrupted the interaction between USP4 and Rheb (Supplementary information, Fig.  xref ), and promoted Rheb ubiquitination (Supplementary information, Fig.  xref )."
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"The interaction between USP4 and Rheb in cells was confirmed by both in vivo co-IP assay and in vitro GST pull down assay (Fig.  xref )."
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"Moreover, our data suggest that the DUSP domain of USP4 accounts for the interaction between Rheb and USP4 (Supplementary information, Fig.  xref )."
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