IndraLab
Statements
reach
"Furthermore, forced USP12 expression significantly potentiated the protein stability of PPM1B (Supplementary Fig. 3d), and the knockdown of WDR48, a binding partner of USP12 that was reported to contribute to USP12 deubiquitinase activity , suppressed PPM1B expression (Supplementary Fig. 3e)."
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
reach
"Highlights : Androgen receptor is a key transcriptional regulator in prostate cancer Usp12, Uaf-1, and WDR20 complex plays a crucial role in androgen receptor stability and activity Destabilising an individual Usp12, Uaf-1, and WDR20 complex member reduces the protein levels of the whole complex and diminishes androgen receptor activity Protein levels of all members of the Usp12, Uaf-1, and WDR20 complex are significantly increased in PC INTRODUCTION."
reach
"For example, silencing of USP12 cofactors, Usp1-associated factor 1 (UAF1), or WD repeat domain 20 (WDR20), could influence the UAF1/WDR20/USP12 complex, thus inhibiting USP12 activity and AR-mediated transcription, leading to attenuated colony formation and promoting apoptosis [105]."
sparser
"Recent structural work revealed how the WD-repeat protein UAF1 interacted with the catalytic domain of USP46 and USP12, which is facilitated predominantly through the “fingers” subdomain of the USP core, mediating long-range allosteric interactions eventually leading to enhanced enzyme efficiency ( xref , xref )."
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
PHLPP1 binds MTBMA_c15380, WDR48, and USP12. 1 / 1
|
1
reach
"Furthermore, forced USP12 expression significantly potentiated the protein stability of PPM1B (Supplementary Fig. 3d), and the knockdown of WDR48, a binding partner of USP12 that was reported to contribute to USP12 deubiquitinase activity , suppressed PPM1B expression (Supplementary Fig. 3e)."
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
reach
"Highlights : Androgen receptor is a key transcriptional regulator in prostate cancer Usp12, Uaf-1, and WDR20 complex plays a crucial role in androgen receptor stability and activity Destabilising an individual Usp12, Uaf-1, and WDR20 complex member reduces the protein levels of the whole complex and diminishes androgen receptor activity Protein levels of all members of the Usp12, Uaf-1, and WDR20 complex are significantly increased in PC INTRODUCTION."
reach
"For example, silencing of USP12 cofactors, Usp1-associated factor 1 (UAF1), or WD repeat domain 20 (WDR20), could influence the UAF1/WDR20/USP12 complex, thus inhibiting USP12 activity and AR-mediated transcription, leading to attenuated colony formation and promoting apoptosis [105]."
sparser
"Recent structural work revealed how the WD-repeat protein UAF1 interacted with the catalytic domain of USP46 and USP12, which is facilitated predominantly through the “fingers” subdomain of the USP core, mediating long-range allosteric interactions eventually leading to enhanced enzyme efficiency ( xref , xref )."
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
PHLPP1 binds MTBMA_c15380, WDR48, and USP12. 1 / 1
|
1
reach
"It raises the possibility that a targeted knockout of Uaf1 in granulosa cells might lead to infertility in female mice, which could provide further insights into UAF1’s specific functions in female reproductive biology.UAF1 acts as a cofactor for USP1, USP12, and USP46, enhancing their deubiquitinase activity by forming stable UAF1/USP protein complexes.14 18 Defective spermiogenesis in Uaf1 sKO mice necessitated the identification of a DUB critical for this process."
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
reach
"Highlights : Androgen receptor is a key transcriptional regulator in prostate cancer Usp12, Uaf-1, and WDR20 complex plays a crucial role in androgen receptor stability and activity Destabilising an individual Usp12, Uaf-1, and WDR20 complex member reduces the protein levels of the whole complex and diminishes androgen receptor activity Protein levels of all members of the Usp12, Uaf-1, and WDR20 complex are significantly increased in PC INTRODUCTION."
reach
"For example, silencing of USP12 cofactors, Usp1-associated factor 1 (UAF1), or WD repeat domain 20 (WDR20), could influence the UAF1/WDR20/USP12 complex, thus inhibiting USP12 activity and AR-mediated transcription, leading to attenuated colony formation and promoting apoptosis [105]."
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
reach
"Highlights : Androgen receptor is a key transcriptional regulator in prostate cancer Usp12, Uaf-1, and WDR20 complex plays a crucial role in androgen receptor stability and activity Destabilising an individual Usp12, Uaf-1, and WDR20 complex member reduces the protein levels of the whole complex and diminishes androgen receptor activity Protein levels of all members of the Usp12, Uaf-1, and WDR20 complex are significantly increased in PC INTRODUCTION."
reach
"For example, silencing of USP12 cofactors, Usp1-associated factor 1 (UAF1), or WD repeat domain 20 (WDR20), could influence the UAF1/WDR20/USP12 complex, thus inhibiting USP12 activity and AR-mediated transcription, leading to attenuated colony formation and promoting apoptosis [105]."
reach
"H. Model : UAF1 mediates the interaction between USP1 and RAD51AP1.of USP12 and USP46, 2 DUBs that also associate with UAF1,26 did not significantly affect the RAD51AP1 stability, suggesting that the effect is specific to the USP1-UAF1 complex.Mapping of the UAF1 interacting region of RAD51AP1In order to specifically determine the functional significance of the UAF1-RAD51AP1 interaction, we sought to identify the residues within RAD51AP1 required for interacting with UAF1."
reach
"Although the PHLLP1 and PHLLP2 phosphatases have been reported to be components of the USP46 and USP12 complexes, they were not detectably associated with EBNA3 complexes, likely because these phosphatases are predominantly membrane associated [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."
sparser
"Recent structural work revealed how the WD-repeat protein UAF1 interacted with the catalytic domain of USP46 and USP12, which is facilitated predominantly through the “fingers” subdomain of the USP core, mediating long-range allosteric interactions eventually leading to enhanced enzyme efficiency ( xref , xref )."
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
reach
"H. Model : UAF1 mediates the interaction between USP1 and RAD51AP1.of USP12 and USP46, 2 DUBs that also associate with UAF1,26 did not significantly affect the RAD51AP1 stability, suggesting that the effect is specific to the USP1-UAF1 complex.Mapping of the UAF1 interacting region of RAD51AP1In order to specifically determine the functional significance of the UAF1-RAD51AP1 interaction, we sought to identify the residues within RAD51AP1 required for interacting with UAF1."
reach
"Although the PHLLP1 and PHLLP2 phosphatases have been reported to be components of the USP46 and USP12 complexes, they were not detectably associated with EBNA3 complexes, likely because these phosphatases are predominantly membrane associated [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."
sparser
"Recent structural work revealed how the WD-repeat protein UAF1 interacted with the catalytic domain of USP46 and USP12, which is facilitated predominantly through the “fingers” subdomain of the USP core, mediating long-range allosteric interactions eventually leading to enhanced enzyme efficiency ( xref , xref )."
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
reach
"This further confirms that each complex member alone has a limited effect on AR activity but in combination the Usp12 complex significantly increases AR transcriptional activity implying that targeting any of the binding partners should offer the same efficacy as targeting Usp12 alone."
reach
"Our previous observations for the LNCaP cell line that express AR FL but not AR Vs have demonstrated that USP12 silencing causes a decrease in transcript expression of all the AR target genes [XREF_BIBR] supporting our data that AR Vs are not targeted by USP12 in the AR V expressing CWR22Rv1 cell line."
reach
"Our previous observations for the LNCaP cell line that express AR FL but not AR Vs have demonstrated that USP12 silencing causes a decrease in transcript expression of all the AR target genes [XREF_BIBR] supporting our data that AR Vs are not targeted by USP12 in the AR V expressing CWR22Rv1 cell line."
|
11
18
sparser
"For example, USP12 is described above as promoting NF-κB activation for NLRP3 inflammatory microsome production, but in NSCLC it inhibits NF-κB signaling activity and regulates chemokine secretion by deubiquitinating and stabilizing PPM1B. Apart from that, USP12 can translocate from the cytoplasm to the nucleus during signaling to function in maintaining nuclear p-STAT1 levels and IFN antiviral efficacy."
sparser
"After stimulating the TCR with an anti-CD3 antibody, phosphorylation of the USP12 cytoplasmic pool could be induced, allowing USP12 to translocate from the nucleus to the cytoplasm and acted directly on the substrate proteins LAT and Trat1 to stabilize the TCR complex on the T cell surface during signaling."
reach
"Importantly, overexpression of Usp12 deubiquitinated both PHLPP and PHLPPL and this elevated the steady-state levels of the enzymes, while overexpression of an enzymatically inactive Usp12 C48A mutant failed to elevate PHLPP and PHLPPL levels suggesting the importance of Usp12 enzymatic activity for phosphatase regulation."
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
reach
"Highlights : Androgen receptor is a key transcriptional regulator in prostate cancer Usp12, Uaf-1, and WDR20 complex plays a crucial role in androgen receptor stability and activity Destabilising an individual Usp12, Uaf-1, and WDR20 complex member reduces the protein levels of the whole complex and diminishes androgen receptor activity Protein levels of all members of the Usp12, Uaf-1, and WDR20 complex are significantly increased in PC INTRODUCTION."
reach
"For example, silencing of USP12 cofactors, Usp1-associated factor 1 (UAF1), or WD repeat domain 20 (WDR20), could influence the UAF1/WDR20/USP12 complex, thus inhibiting USP12 activity and AR-mediated transcription, leading to attenuated colony formation and promoting apoptosis [105]."
USP12 affects cell population proliferation
|
18
USP12 activates cell population proliferation.
|
11
USP12 activates cell population proliferation. 10 / 11
|
11
reach
"On the other hand, USP12 promoted prostate cancer (PC) cells proliferation by forming a complex with Uaf-1 and WDR20 and deubiquitinating AR, thereby increasing AR stability and transcriptional activity, whereas USP12 silencing led to a significant decrease in PC cell proliferation [42, 64]."
USP12 inhibits cell population proliferation.
|
7
USP12 inhibits cell population proliferation. 7 / 9
|
7
reach
"Several studies reported the effects of USP12 on cell phenotypes, and we give a summary of the roles and mechanisms of USP12 in tumorigenesis in the following part.The overexpression of USP12 in human colorectal cancer cells was previously found to inhibit cell proliferation, and siUSP12 could reverse this effect [63]."
reach
"In this study, we found that USP12 significantly inhibited IFI16 ubiquitination and degradation, stabilized and extended the half-life of IFI16, and subsequently induced the STING-dependent expressions of IFN-β, CCL-5, IL-6, and downstream ISGs, which makes the host more resistant to DNA virus (S11 Fig)."
reach
"The diverse regulation of cell proliferation and cell cycle by USP12 indicates its functional diversity and deserves further study.USP12 silencing could induce the upregulation of Bax in prostate cancer by regulating the TP53 signaling pathway [68], and in Hela cells, Bax could bind to USP12 in the nucleus [50]."
USP12 affects apoptotic process
|
1
14
USP12 activates apoptotic process.
|
1
8
USP12 activates apoptotic process. 9 / 9
|
1
8
reach
"In this study, we identified significant upregulation of the m 6 A methyltransferase METTL3 (methyltransferase-like 3), the m 6 A reader protein YTHDF1 (YTH N6-methyladenosine RNA binding protein 1), as well as increased expression levels of USP12 (ubiquitin-specific peptidase 12), FOXO3 (forkhead box O3), and key molecules in the intrinsic apoptotic pathway, PUMA (p53 upregulated modulator of apoptosis) and BAX (Bcl-2-associated X), through proteomic profiling in an LPS (Lipopolysaccharide)-induced SIMD mouse model."
reach
"Invivo experiments showed that USP12-knockdown could suppress tumor growth in mice, and immuno blotting revealed that USP12 could induce G2/M arrest through the cyclin dependent kinase1 and cyclinB1 axis, and trigger apoptosis via the p38 and mitogen activated protein kinase pathway."
eidos
"In vivo experiments showed that USP12-knockdown could suppress tumor growth in mice , and immuno-blotting revealed that USP12 could induce G2 / M arrest through the cyclin dependent kinase 1 / cyclinB1 axis , and trigger apoptosis via the p38 / mitogen-activated protein kinase pathway ."
USP12 inhibits apoptotic process.
|
6
sparser
"Recent structural work revealed how the WD-repeat protein UAF1 interacted with the catalytic domain of USP46 and USP12, which is facilitated predominantly through the “fingers” subdomain of the USP core, mediating long-range allosteric interactions eventually leading to enhanced enzyme efficiency ( xref , xref )."
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
USP12 affects Hypertrophy
|
10
USP12 activates Hypertrophy.
|
8
USP12 inhibits Hypertrophy.
|
2
UAF1 affects USP12
|
10
eidos
"Highlights d Free USP12 deubiquitinase is inactive and has several flexible structural elements d UAF1 and WDR20 can both activate USP12 without increasing its substrate affinity d UAF1 and WDR20 bind USP12 at two distinct sites away from its catalytic center d Two distinct allosteric mechanisms underlie USP12 activation by UAF1 and WDR20 Ubiquitin-specific proteases ( USPs ) constitute the largest family of deubiquitinating enzymes , whose catalytic competency is often modulated by their binding partners through unknown mechanisms ."
sparser
"Recent structural work revealed how the WD-repeat protein UAF1 interacted with the catalytic domain of USP46 and USP12, which is facilitated predominantly through the “fingers” subdomain of the USP core, mediating long-range allosteric interactions eventually leading to enhanced enzyme efficiency ( xref , xref )."
USP12 affects Neoplasm Invasiveness
|
1
7
USP12 activates Neoplasm Invasiveness.
|
1
5
USP12 inhibits Neoplasm Invasiveness.
|
2
USP12 affects Histone_H2B
|
8
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
reach
"The dauer larva-constitutive C. elegans phenotype caused by defective DAF-7 and TGF-beta signaling was enhanced and suppressed, respectively, by ubh-1 deletion and overexpression in the loss-of-function genetic backgrounds of daf7, daf-1 and TGF-betaRI, and daf4 and R-SMAD, but not of daf-8 and R-SMAD."
reach
"Here, we show that the deubiquitinating enzyme UBH-1 in Caenorhabditis elegans and its human homolog, ubiquitin C-terminal hydrolase-L1 (UCH-L1), stimulate DAF-7 and TGF-beta signaling, suggesting that this mode of regulation of TGF-beta signaling is conserved across animal species."
reach
"The dauer larva-constitutive C. elegans phenotype caused by defective DAF-7 and TGF-beta signaling was enhanced and suppressed, respectively, by ubh-1 deletion and overexpression in the loss-of-function genetic backgrounds of daf7, daf-1 and TGF-betaRI, and daf4 and R-SMAD, but not of daf-8 and R-SMAD."
reach
"Here, we show that the deubiquitinating enzyme UBH-1 in Caenorhabditis elegans and its human homolog, ubiquitin C-terminal hydrolase-L1 (UCH-L1), stimulate DAF-7 and TGF-beta signaling, suggesting that this mode of regulation of TGF-beta signaling is conserved across animal species."
USP12 affects Prostatic Neoplasms
|
7
USP12 activates Prostatic Neoplasms.
|
5
USP12 activates Prostatic Neoplasms. 5 / 5
|
5
reach
"On the other hand, USP12 promoted prostate cancer (PC) cells proliferation by forming a complex with Uaf-1 and WDR20 and deubiquitinating AR, thereby increasing AR stability and transcriptional activity, whereas USP12 silencing led to a significant decrease in PC cell proliferation [42, 64]."
USP12 inhibits Prostatic Neoplasms.
|
1
USP12 inhibits Prostatic Neoplasms. 1 / 1
|
1
USP12 deubiquitinates Prostatic Neoplasms.
|
1
USP12 deubiquitinates Prostatic Neoplasms. 1 / 1
|
1
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
USP12 affects cell cycle
|
2
4
USP12 activates cell cycle.
|
1
4
USP12 inhibits cell cycle.
|
1
USP12 inhibits cell cycle. 1 / 1
|
1
reach
"After stimulating the TCR with an anti-CD3 antibody, phosphorylation of the USP12 cytoplasmic pool could be induced, allowing USP12 to translocate from the nucleus to the cytoplasm and acted directly on the substrate proteins LAT and Trat1 to stabilize the TCR complex on the T cell surface during signaling."
reach
"In contrast, re-expression of the catalytically inactive USP12 or USP46 mutants, in which the catalytic site cysteine was substituted for serine (Li et al, 2016; Yin et al, 2015), were unable to restore the Itgb1 levels indicating that USP12/46 require the DUB activity to maintain the 125 kDa mature Itgb1 levels (Figs."
reach
"Expression of USP12 in USP12/46-dKO fibroblasts rescued Itgb1 surface levels, whereas expression of USP12 or USP12 only partially rescued Itgb1 surface levels and expression of USP12 did not increase Itgb1 levels beyond the levels of USP12/46-dKO fibroblasts expressing EGFP-only (Figs."
reach
"Given that USP12 negatively regulated protumourigenic chemokine expression and exerted a tumour-suppressive effect in immune-competent mice but not in immune-deficient mice, it seems intuitive to speculate that USP12 downregulation in tumours may help establish a favourable microenvironment for tumour growth."
reach
"This study demonstrated that AKT-mTOR hyperactivation resulted in USP12 downregulation, which in turn increased the production of protumourigenic chemokine in NSCLC, indicating that USP12 is a regulatory node in oncogenic mutation-driven TME development.The tumour suppressive function conveyed by USP12 is virtually related to the control of protumourigenic chemokine expression."
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
USP12 affects defense response to virus
|
5
reach
"Here, we show that the deubiquitinating enzyme UBH-1 in Caenorhabditis elegans and its human homolog, ubiquitin C-terminal hydrolase-L1 (UCH-L1), stimulate DAF-7 and TGF-beta signaling, suggesting that this mode of regulation of TGF-beta signaling is conserved across animal species."
reach
"Here, we show that the deubiquitinating enzyme UBH-1 in Caenorhabditis elegans and its human homolog, ubiquitin C-terminal hydrolase-L1 (UCH-L1), stimulate DAF-7 and TGF-beta signaling, suggesting that this mode of regulation of TGF-beta signaling is conserved across animal species."
USP12 affects Interferon
|
5
USP12 activates Interferon.
|
4
USP12 increases the amount of Interferon.
|
1
USP12 increases the amount of Interferon. 1 / 1
|
1
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
USP12 affects regulation of cell cycle
|
4
USP12 inhibits regulation of cell cycle.
|
2
USP12 activates regulation of cell cycle.
|
2
USP12 affects ossification
|
4
USP12 inhibits ossification.
|
2
USP12 activates ossification.
|
2
USP12 affects mHTT
|
4
USP12 affects lipopolysaccharide
|
4
USP12 affects angiogenesis
|
4
USP12 activates angiogenesis.
|
3
USP12 inhibits angiogenesis.
|
1
USP12 inhibits angiogenesis. 1 / 1
|
1
reach
"Knockdown of USP12 inhibited the NF-κB pathway by reducing the phosphorylation level of IkBa (degraded form), which was a kind of inhibitor of NF-κB nuclear translocation in LPS-induced macrophages, and an increased number of dephosphorylated IkBa was then translocated to the nucleus to inhibit the NF-κB pathway."
Valproic acid affects USP12
3
|
Valproic acid decreases the amount of USP12.
2
|
Valproic acid methylates USP12.
1
|
USP12 affects cell growth
|
1
2
USP12 activates cell growth.
|
1
1
USP12 inhibits cell growth.
|
1
USP12 inhibits cell growth. 1 / 1
|
1
USP12 affects Neoplasm Metastasis
|
3
USP12 affects ISG
|
3
USP12 affects Cell Survival
|
1
2
USP12 activates Cell Survival.
|
1
1
USP12 inhibits Cell Survival.
|
1
USP12 inhibits Cell Survival. 1 / 1
|
1
USP12 affects Carcinoma, Non-Small-Cell Lung
|
1
2
USP12 activates Carcinoma, Non-Small-Cell Lung.
|
1
1
USP12 inhibits Carcinoma, Non-Small-Cell Lung.
|
1
USP12 inhibits Carcinoma, Non-Small-Cell Lung. 1 / 1
|
1
USP12 affects Angiotensin-2
|
3
USP12 is modified
|
1
2
reach
"Consistently, ectopic expression of the myristoylated form of AKT1 (myr-AKT1) significantly repressed USP12 expression in both human and mouse tumour cells (Fig. 1k); knockdown of endogenous AKT1 or AKT2, the two major isoforms of AKT, induced higher levels of USP12 than did the control treatment (Fig. 1l)."
Pirinixic acid affects USP12
2
|
MHTT affects USP12
|
2
Bisphenol A affects USP12
2
|
Bisphenol A methylates USP12.
1
|
Bisphenol A decreases the amount of USP12.
1
|
WDR48 affects MTBMA_c15380
|
2
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
PHLPP1 binds MTBMA_c15380, WDR48, and USP12. 1 / 1
|
1
WDR proteins affects USP12
|
2
Vehicle Emissions affects USP12
2
|
Usp12-C48S affects USP12
|
2
USP12 affects signal transduction
|
2
USP12 affects mHTT-N586
|
2
USP12 affects interleukin-6 production
|
2
USP12 affects cell migration
|
2
USP12 affects WDR proteins
|
2
USP12 affects Usp12-C48S
|
2
USP12 decreases the amount of USP12.
|
1
USP12 activates USP12.
|
1
reach
"After stimulating the TCR with an anti-CD3 antibody, phosphorylation of the USP12 cytoplasmic pool could be induced, allowing USP12 to translocate from the nucleus to the cytoplasm and acted directly on the substrate proteins LAT and Trat1 to stabilize the TCR complex on the T cell surface during signaling."
reach
"It raises the possibility that a targeted knockout of Uaf1 in granulosa cells might lead to infertility in female mice, which could provide further insights into UAF1’s specific functions in female reproductive biology.UAF1 acts as a cofactor for USP1, USP12, and USP46, enhancing their deubiquitinase activity by forming stable UAF1/USP protein complexes.14 18 Defective spermiogenesis in Uaf1 sKO mice necessitated the identification of a DUB critical for this process."
USP12 affects Proteasome
|
2
USP12 affects Phosphatase
|
2
USP12 deubiquitinates Phosphatase. 1 / 1
|
1
USP12 deubiquitinates Phosphatase on leucine. 1 / 1
|
1
USP12 affects Multiple Myeloma
|
2
USP12 affects MTBMA_c15380
|
2
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
PHLPP1 binds MTBMA_c15380, WDR48, and USP12. 1 / 1
|
1
USP12 affects ISRE
|
2
USP12 affects ISGs
|
2
USP12 affects Endoplasmic Reticulum Stress
|
2
USP12 inhibits Endoplasmic Reticulum Stress.
|
1
USP12 inhibits Endoplasmic Reticulum Stress. 1 / 1
|
1
USP12 activates Endoplasmic Reticulum Stress.
|
1
USP12 activates Endoplasmic Reticulum Stress. 1 / 1
|
1
USP12 affects DNA replication
|
2
USP12 affects Ang II-induced cardiac hypertrophy
|
2
sparser
"Although the exact function of p80/Uaf1 in viral DNA replication remains unclear, this WD40 repeats-containing protein is known to interact with the de-ubiquitinating enzymes Usp1, Usp12 and Usp46, whose substrates include PCNA, histones H2A and H2B and Fanconi anaemia complementation group D type 2, a component of the Fanconi anaemia pathway [ xref , xref ]."
MTBMA_c15380 affects WDR48
|
2
WDR48 binds MTBMA_c15380, USP12, and USP46. 1 / 1
|
1
PHLPP1 binds MTBMA_c15380, WDR48, and USP12. 1 / 1
|
1
Infections affects USP12
|
2
Infections increases the amount of USP12. 2 / 2
|
2
reach
"To explore the potential role of USP12 in antiviral immunity, we first detected the expression of USP12 after virus infection and found that USP12 expression was induced by HSV-1 infection (S1A and S1B Fig), which was mainly regulated by NF-κB p65 and IRF3, but not by STAT1 (S1C and S1D Fig)."
17beta-estradiol affects USP12
2
|
Vorinostat affects USP12
1
|
1
|
Trichloroethene affects USP12
1
|
Transmitting long-range allosteric signal BL1 affects USP12
|
1
Testosterone affects USP12
1
|
Substrate affinity affects USP12
|
1
Sodium arsenite affects USP12
1
|
reach
"Treatment with inhibitors targeting MEK (U0126), ERK (ERK inhibitor), JNK (JNK inhibitor II), JAK-STAT3 (WP1066), c-Myc (c-Myc inhibitor), p38 MAPK (SB203580), and TGF-β-SMAD (SB431542 and SIS3) failed to produce a substantial change in USP12 expression (Supplementary Fig. 1e); however, blocking AKT-mTOR signalling using the selective AKT inhibitor API-2 or the mTOR inhibitor rapamycin significantly increased USP12 expression (Fig. 1i, j)."
Silicon dioxide affects USP12
1
|
Potassium chromate affects USP12
1
|
Phorbol 13-acetate 12-myristate affects USP12
1
|
Perfluorooctane-1-sulfonic acid affects USP12
1
|
Paracetamol affects USP12
1
|
Optimizing catalytic cleft affects USP12
|
1
Okadaic acid affects USP12
1
|
Mutation Ub globular domain-binding residue BL1 affects USP12
|
1
eidos
"Finally , consistent with the expected substrate-induced interactions between Ub and BL1 of USP12 in all forms , mutation of a Ub globular domain-binding residue on BL1 , Y264 , compromised the ability of both UAF1 and WDR20 to activate USP12 for Ub-AMC hydrolysis ( Figures 6D , 6E , and S5F ) ."
Medroxyprogesterone acetate affects USP12
1
|
MTOR inhibitor affects USP12
|
1
MTOR inhibitor decreases the amount of USP12. 1 / 1
|
1
reach
"Treatment with inhibitors targeting MEK (U0126), ERK (ERK inhibitor), JNK (JNK inhibitor II), JAK-STAT3 (WP1066), c-Myc (c-Myc inhibitor), p38 MAPK (SB203580), and TGF-β-SMAD (SB431542 and SIS3) failed to produce a substantial change in USP12 expression (Supplementary Fig. 1e); however, blocking AKT-mTOR signalling using the selective AKT inhibitor API-2 or the mTOR inhibitor rapamycin significantly increased USP12 expression (Fig. 1i, j)."
Lipopolysaccharide affects USP12
|
1
Lipopolysaccharide activates USP12. 1 / 1
|
1
Leflunomide affects USP12
1
|
Hypochlorous acid affects USP12
1
|
Hsa-mir-4533 affects USP12
1
|
Hsa-mir-4476 affects USP12
1
|
Hsa-mir-4447 affects USP12
1
|
Hsa-mir-4271 affects USP12
1
|
Hsa-miR-9500 affects USP12
1
|
Hsa-miR-92a-2-5p affects USP12
1
|
Hsa-miR-8083 affects USP12
1
|
Hsa-miR-744-5p affects USP12
1
|
Hsa-miR-6887-5p affects USP12
1
|
Hsa-miR-6885-5p affects USP12
1
|
Hsa-miR-6876-5p affects USP12
1
|
Hsa-miR-6795-5p affects USP12
1
|
Hsa-miR-6780b-5p affects USP12
1
|
Hsa-miR-625-5p affects USP12
1
|
Hsa-miR-5196-5p affects USP12
1
|
Hsa-miR-4747-5p affects USP12
1
|
Hsa-miR-4725-3p affects USP12
1
|
Hsa-miR-4472 affects USP12
1
|
Hsa-miR-373-3p affects USP12
1
|
Hsa-miR-328-5p affects USP12
1
|
Hsa-miR-3202 affects USP12
1
|
Hsa-miR-215-5p affects USP12
1
|
Hsa-miR-192-5p affects USP12
1
|
Formaldehyde affects USP12
1
|
Folic acid affects USP12
1
|
Doxorubicin affects USP12
1
|
Deltamethrin affects USP12
1
|
Cycloheximide affects USP12
|
1
Cycloheximide inhibits USP12. 1 / 1
|
1
Cre affects USP12
|
1
Clofibrate affects USP12
1
|
Capsid protein affects USP12
|
1
USP12 binds capsid protein. 1 / 1
|
1
Bucladesine affects USP12
1
|
Bisphenol F affects USP12
1
|
Binding tip Fingers domain affects USP12
|
1
Beta-D-glucose affects USP12
1
|
Benzo[a]pyrene affects USP12
1
|
Avobenzone affects USP12
1
|
Aristolochic acid A affects USP12
1
|
Aluminium atom affects USP12
1
|
All-trans-retinoic acid affects USP12
1
|
Aflatoxin B1 affects USP12
1
|
WDR48-WDR20 affects USP12
|
1
WDR48 affects F219
|
1
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
WDR20 affects 293 T
|
1
WD40-repeat-containing protein affects USP12
|
1
Vincristine affects USP12
1
|
eidos
"Highlights d Free USP12 deubiquitinase is inactive and has several flexible structural elements d UAF1 and WDR20 can both activate USP12 without increasing its substrate affinity d UAF1 and WDR20 bind USP12 at two distinct sites away from its catalytic center d Two distinct allosteric mechanisms underlie USP12 activation by UAF1 and WDR20 Ubiquitin-specific proteases ( USPs ) constitute the largest family of deubiquitinating enzymes , whose catalytic competency is often modulated by their binding partners through unknown mechanisms ."
USP46 affects MTBMA_c15380
|
1
USP12 affects tumor growth
|
1
USP12 affects transduction
|
1
USP12 inhibits transduction. 1 / 1
|
1
reach
"Thus, USP12 and OTUD7B counterbalance inhibitory E3 ligases to stabilize TCR signaling.Aside from acting at the proximal signalosome, E3 ligases also inhibit T-cell activation by acting further downstream, controlling the key transduction events that lead to the nuclear translocation of the T-cell activating transcription factors."
| PMC
USP12 affects transcription downstream METTL3
|
1
USP12 affects receptor complex
|
1
USP12 affects pathways
|
1
USP12 affects pathogenesis
|
1
USP12 activates pathogenesis. 1 / 1
|
1
USP12 affects nitric oxide
|
1
USP12 decreases the amount of nitric oxide. 1 / 1
|
1
USP12 affects marker) protein
|
1
USP12 affects macrophage activation
|
1
USP12 activates macrophage activation. 1 / 1
|
1
USP12 affects function monocytic MDSCs
|
1
USP12 affects function M-MDSCs
|
1
|
1
USP12 inhibits cytokine-mediated signaling pathway. 1 / 1
|
1
reach
"Gene ontology (GO) analysis revealed that the pathways significantly enriched in USP12-downregulated genes were mostly related to cytokine-mediated signalling pathway and immune effector process (Fig. 3a); further in-depth examination showed that USP12 expression decreased levels of a number of chemokine, such as CXCL8, CXCL1, CXCL2, CCL2 and CCL5, most of which were associated with immune cell recruitment (Fig. 3b and Supplementary Fig. 2a)."
USP12 affects cre
|
1
USP12 affects clonogenicity
|
1
USP12 affects chemokine production
|
1
USP12 inhibits chemokine production. 1 / 1
|
1
USP12 affects cellular senescence
|
1
USP12 activates cellular senescence. 1 / 1
|
1
USP12 affects cell cycle protein
|
1
USP12 increases the amount of cell cycle protein. 1 / 1
|
1
USP12 affects capsid protein
|
1
USP12 binds capsid protein. 1 / 1
|
1
USP12 affects bortezomib
|
1
USP12 activates bortezomib. 1 / 1
|
1
USP12 affects WDR48.USP12 complex
|
1
USP12 affects WDR48-WDR20
|
1
USP12 affects VSV-
|
1
USP12 affects UPR-related genes
|
1
USP12 affects UAF
|
1
USP12 affects T cell activation
|
1
USP12 inhibits T cell activation. 1 / 1
|
1
reach
"Thus, USP12 and OTUD7B counterbalance inhibitory E3 ligases to stabilize TCR signaling.Aside from acting at the proximal signalosome, E3 ligases also inhibit T-cell activation by acting further downstream, controlling the key transduction events that lead to the nuclear translocation of the T-cell activating transcription factors."
| PMC
USP12 affects Snail1
|
1
USP12 affects Sf9
|
1
USP12 affects S2B
|
1
USP12 affects PHLPP1.WDR48
|
1
USP12 affects P53-MDM2-AR-AKT
|
1
USP12 affects NOTCH receptors
|
1
USP12 affects MTLL3
|
1
USP12 affects MDM2-P53
|
1
USP12 affects Infections
|
1
USP12 inhibits Infections. 1 / 1
|
1
USP12 affects ISRE promoter
|
1
USP12 affects ISG65
|
1
USP12 affects Huntington Disease
|
1
USP12 activates Huntington Disease. 1 / 1
|
1
USP12 affects Hippo
|
1
USP12 affects Herpes Simplex
|
1
USP12 activates Herpes Simplex. 1 / 1
|
1
USP12 affects HTT fragments
|
1
USP12 affects HMGB1-mediated autophagy
|
1
USP12 affects HIPPO
|
1
USP12 affects H2BK120ub
|
1
USP12 affects G0/G1
|
1
USP12 affects F219
|
1
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
sparser
"Since EGFP-USP12 was absent from FAs (Fig. xref ) and the members of the USP12/46-WDRs complex were also undetectable in the integrin adhesome (Horton et al, xref ; Kuo et al, xref ; Schiller et al, xref ), we conclude that the USP12/46-WDRs complex binds Itgb1 on endosomes and not in FAs."
USP12 affects E3_Ub_ligase
|
1
E3_Ub_ligase binds USP12. 1 / 1
|
1
USP12 affects Deubiquitinase
|
1
USP12 inhibits Deubiquitinase. 1 / 1
|
1
USP12 affects Delta-actitoxin-Avd1a
|
1
Delta-actitoxin-Avd1a binds USP12. 1 / 1
|
1
sparser
"In xref , we visualized the top 50 negatively correlated expression genes, and the top five are presented in the form of scatter plots in xref : AL355075.4 ( r = -0.459), AC087286.1 ( r = - 0.558), AC100800.1 ( r = -0.534), ZBTB20-AS5 ( r = -0.534), and USP12-AS1 ( r = -0.566), all with P -value <0.001."
USP12 affects DNA-templated transcription
|
1
USP12 activates DNA-templated transcription. 1 / 1
|
1
USP12 affects Carcinogenesis
|
1
USP12 activates Carcinogenesis. 1 / 1
|
1
USP12 affects CD4+
|
1
USP12 affects Ang II-induced myocardial hypertrophy
|
1
USP12 affects AKT-mTOR
|
1
USP12 affects AKT phosphatase
|
1
USP12 affects 293 T
|
1
UAF affects USP12
|
1
Tobacco Smoke Pollution affects USP12
1
|
Small hairpin affects USP12
|
1
reach
"XREF_BIBR, XREF_BIBR In addition, other E3 ubiquitin ligases are probably involved in the AR regulation process, such as CHIP XREF_BIBR and SKP2 XREF_BIBR which also promote AR degradation, and Siah2, XREF_BIBR RNF6, XREF_BIBR and USP12 XREF_BIBR, XREF_BIBR which promote deubiquitination and AR activation."
reach
"XREF_BIBR, XREF_BIBR In addition, other E3 ubiquitin ligases are probably involved in the AR regulation process, such as CHIP XREF_BIBR and SKP2 XREF_BIBR which also promote AR degradation, and Siah2, XREF_BIBR RNF6, XREF_BIBR and USP12 XREF_BIBR, XREF_BIBR which promote deubiquitination and AR activation."
sparser
"Somatic copy‐number analyses and epigenetic analyses of 12 cancer cell types revealed that SEs generated by focal amplification could aberrantly activate KLF5 , USP12 , PARD6B , or MYC . xref Another study also revealed that the 350‐2000 kb genomic region, including the MYCN oncogene, was amplified aberrantly in neuroblastoma, resulting in the activation of MYCN expression. xref "
1
|
eidos
"Highlights d Free USP12 deubiquitinase is inactive and has several flexible structural elements d UAF1 and WDR20 can both activate USP12 without increasing its substrate affinity d UAF1 and WDR20 bind USP12 at two distinct sites away from its catalytic center d Two distinct allosteric mechanisms underlie USP12 activation by UAF1 and WDR20 Ubiquitin-specific proteases ( USPs ) constitute the largest family of deubiquitinating enzymes , whose catalytic competency is often modulated by their binding partners through unknown mechanisms ."
PHLPP1.WDR48 affects USP12
|
1
PHLPP1 affects MTBMA_c15380
|
1
MTBMA_c15380 affects USP46
|
1
MTBMA_c15380 affects PHLPP1, USP12, and WDR48
|
1
Figure affects USP12
|
1
F219 affects WDR48
|
1
reach
"While the Pinky Finger of USP12 in the UAF1, USP12, and WDR20 complex adopts the same beta-strand conformation as seen in the UAF1 and USP12 complex, F219 on the PK Helix becomes embedded in its pocket formed between the Fingers and Palm domains, instead of being tucked underneath the Fingers domain (XREF_FIG, XREF_FIG, XREF_FIG)."
sparser
"Since EGFP-USP12 was absent from FAs (Fig. xref ) and the members of the USP12/46-WDRs complex were also undetectable in the integrin adhesome (Horton et al, xref ; Kuo et al, xref ; Schiller et al, xref ), we conclude that the USP12/46-WDRs complex binds Itgb1 on endosomes and not in FAs."
E3_Ub_ligase affects USP12
|
1
E3_Ub_ligase binds USP12. 1 / 1
|
1
Delta-actitoxin-Avd1a affects USP12
|
1
Delta-actitoxin-Avd1a binds USP12. 1 / 1
|
1
sparser
"In xref , we visualized the top 50 negatively correlated expression genes, and the top five are presented in the form of scatter plots in xref : AL355075.4 ( r = -0.459), AC087286.1 ( r = - 0.558), AC100800.1 ( r = -0.534), ZBTB20-AS5 ( r = -0.534), and USP12-AS1 ( r = -0.566), all with P -value <0.001."
DEHP A2780 cells affects USP12
|
1
1
|
sparser
"Other examples of protein complexes retrieved include host cell factor-1, galectin 7, p97, ras GTPase binding proteins 1 and 2, WD-repeat containing protein 48 and WD-repeat containing protein 20, and thioredoxin like protein, which interact with BAP, USP1, ataxin-3, USP10, USP12, and USP14 or UCH-L5 [ xref , xref ], respectively."
reach
"Consistently, ectopic expression of the myristoylated form of AKT1 (myr-AKT1) significantly repressed USP12 expression in both human and mouse tumour cells (Fig. 1k); knockdown of endogenous AKT1 or AKT2, the two major isoforms of AKT, induced higher levels of USP12 than did the control treatment (Fig. 1l)."
reach
"Consistently, ectopic expression of the myristoylated form of AKT1 (myr-AKT1) significantly repressed USP12 expression in both human and mouse tumour cells (Fig. 1k); knockdown of endogenous AKT1 or AKT2, the two major isoforms of AKT, induced higher levels of USP12 than did the control treatment (Fig. 1l)."
1
|
1
|
293 T affects WDR20
|
1
1
|