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USP12 increases the amount of METTL3. 8 / 8
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"But the regulatory mechanism of METTL3 in Ang II-induced hypertrophic cardiomyocytes is still unclear.In this study, we demonstrated that the expression level of USP12 was increased in hypertrophic he[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Overexpressing USP12 increased the mRNA and protein expression of METTL3, as well as MAP3K6 and MAPK14 ( Fig. 3 D and E, Supplementary Fig. 3C and D )."
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"For example, USP12 overexpression could exacerbate Ang II-induced cardiac hypertrophy by raising the METTL3 level [51]."
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"Therefore, we speculated that USP12 enhanced METTL3 expression via p300."
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"On the other hand, we transfected the shRNA adenovirus targeting p300 into cells overexpressing USP12 to knock down p300, and we found that reducing p300 attenuated the increase of METTL3 expression a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Indeed, silencing USP12 inhibited the expression of METTL3, overexpression of USP12 enhanced METTL3."
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"In addition, we verified that p300 is the key for USP12-mediated enhancement of METTL3, as overexpression of p300 rescued the decrease of METTL3 expression in USP12-silenced NRCMs, while reducing p300[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"USP12 deubiquitinated p300 to activate the transcription of methyltransferase-like 3 (METTL3), which resulted in the abnormal m6A RNA methylation in cardiomyocyte and exacerbated cardiac hypertrophy (Ref."
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