IndraLab

Statements


USP24 is modified
61 | 20 25
USP24 is phosphorylated.
61 | 17 25
USP24 is phosphorylated. 10 / 28
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rlimsp
"Antibodies that recognized these phosphorylation residues were produced to study USP24 phosphorylation."

sparser
"Phosphorylation of USP24 increases its degradation during tumorigenesis and cell cycle progression."

rlimsp
"Phosphorylation of USP24 at these three residues was increased during mitosis (Figures 7E and F). Moreover, cdc20 could also interact with USP24 when these residues were phosphorylated, implying that the decrease in USP24 during mitosis might be important for cell cycle progression and cancer cell proliferation (Figure 7G)."

rlimsp
"In addition, we found that USP24 phosphorylation at Ser2604 was declined by U0126, which inhibited the Erk1/2 activities in A549 cells (Figure 7J); however, USP24 phosphorylation was not decreased by LY294002, which inhibited the PI3K activities (Supplementary Figure 5F)."

rlimsp
"Our previous liquid chromatography–tandem mass spectrometry (LC/MS/MS) studies probed several phosphorylation residues within USP24 (Supplementary Table 1). The phosphorylation residues were mutated individually; then, we investigated the expression of USP24-wt and its mutants (Figures 7B and C; Supplementary Figure 5B)."

sparser
"Mutations at these residues increased the USP24 half-life during mitosis, suggesting that USP24 phosphorylation during mitosis decreased USP24 expression ( xref )."

sparser
"Antibodies that recognized these phosphorylation residues were produced to study USP24 phosphorylation."

sparser
"Phosphorylation of USP24 at these three residues was increased during mitosis ( xref )."

rlimsp
"Three mutated residues and triple mutant S3A increased the USP24 level, suggesting that USP24 phosphorylation might be the reason for the decreased USP24 expression during mitosis and tumorigenesis."

rlimsp
"Our previous liquid chromatography–tandem mass spectrometry (LC/MS/MS) studies probed several phosphorylation residues within USP24 (Supplementary Table 1). The phosphorylation residues were mutated individually; then, we investigated the expression of USP24-wt and its mutants (Figures 7B and C; Supplementary Figure 5B). Three mutated residues and triple mutant S3A increased the USP24 level, suggesting that USP24 phosphorylation might be the reason for the decreased USP24 expression during mitosis and tumorigenesis. Mutations at these residues increased the USP24 half-life during mitosis, suggesting that USP24 phosphorylation during mitosis decreased USP24 expression (Figure 7D). Antibodies that recognized these phosphorylation residues were produced to study USP24 phosphorylation."
USP24 is phosphorylated on S2047. 10 / 18
16 | 1 1

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rlimsp
"(D) Normal or mitotic A549 cells were collected for an immunoblotting assay with antibodies against USP24, phospho-USP24 (S1616), phospho-USP24 (S2047), phospho-USP24 (S2604), cyclin B1 and actin."

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sparser
"Here we also found that USP24 phosphorylation at Ser1616, Ser2047 and Ser2604 was increased in EGF-treated primary lung cells ( xref )."

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USP24 is phosphorylated on S2604. 10 / 10
3 | 4 3

sparser
"Here we also found that USP24 phosphorylation at Ser1616, Ser2047 and Ser2604 was increased in EGF-treated primary lung cells ( xref )."

sparser
"In addition, we found that USP24 phosphorylation at Ser2604 was declined by U0126, which inhibited the Erk1/2 activities in A549 cells ( xref ); however, USP24 phosphorylation was not decreased by LY294002, which inhibited the PI3K activities ( xref )."

rlimsp
"(D) Normal or mitotic A549 cells were collected for an immunoblotting assay with antibodies against USP24, phospho-USP24 (S1616), phospho-USP24 (S2047), phospho-USP24 (S2604), cyclin B1 and actin."

sparser
"Further study indicated that USP24 could interact with Erk1/2 and p-Erk1/2 ( xref ), suggesting that Erk1/2 might be the kinase for phosphorylation of USP24 at S2604 to decrease the USP24 level."

rlimsp
"In addition, we found that USP24 phosphorylation at Ser2604 was declined by U0126, which inhibited the Erk1/2 activities in A549 cells (Figure 7J); however, USP24 phosphorylation was not decreased by LY294002, which inhibited the PI3K activities (Supplementary Figure 5F)."

rlimsp
"Further study indicated that USP24 could interact with Erk1/2 and p-Erk1/2 (Figure 7K), suggesting that Erk1/2 might be the kinase for phosphorylation of USP24 at S2604 to decrease the USP24 level."

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sparser
"Conversely, USP24 phosphorylation at Ser1616 and Ser2604 was decreased in FTI-276-treated A549 cells ( xref )."
USP24 is phosphorylated on S1616. 7 / 7
2 | 2 3

rlimsp
"(D) Normal or mitotic A549 cells were collected for an immunoblotting assay with antibodies against USP24, phospho-USP24 (S1616), phospho-USP24 (S2047), phospho-USP24 (S2604), cyclin B1 and actin."

rlimsp
"Conversely, USP24 phosphorylation at Ser1616 and Ser2604 was decreased in FTI-276-treated A549 cells (Figure 7I)."

rlimsp
"Here we also found that USP24 phosphorylation at Ser1616, Ser2047 and Ser2604 was increased in EGF-treated primary lung cells (Figure 7H)."

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sparser
"Here we also found that USP24 phosphorylation at Ser1616, Ser2047 and Ser2604 was increased in EGF-treated primary lung cells ( xref )."

sparser
"Conversely, USP24 phosphorylation at Ser1616 and Ser2604 was decreased in FTI-276-treated A549 cells ( xref )."
USP24 is phosphorylated on S2561. 5 / 5
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USP24 is phosphorylated on S251. 5 / 5
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USP24 is phosphorylated on T2565. 4 / 4
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USP24 is phosphorylated on T2559. 3 / 3
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USP24 is phosphorylated on S1943. 2 / 2
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USP24 is phosphorylated on S2080. 2 / 2
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USP24 is phosphorylated on S1610. 2 / 2
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USP24 is phosphorylated on S1143. 2 / 2
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USP24 is phosphorylated on S1141. 2 / 2
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USP24 is phosphorylated on T2570. 2 / 2
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USP24 is phosphorylated on S1612. 2 / 2
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USP24 is phosphorylated on S2077. 2 / 2
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USP24 is phosphorylated on S2081. 1 / 1
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USP24 is phosphorylated on S2587. 1 / 1
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USP24 is phosphorylated on S1043. 1 / 1
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USP24 is phosphorylated on S2613. 1 / 1
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USP24 is phosphorylated on S63. 1 / 1
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USP24 is phosphorylated on S2043. 1 / 1
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USP24 is phosphorylated on S58. 1 / 1
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USP24 is ubiquitinated.
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USP24 is ubiquitinated. 3 / 3
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sparser
"Moreover, the USP24 ubiquitination signal was increased during the mitotic stage ( xref ; xref ), implying that the decrease in the USP24 level during mitosis might be due to the enhancement of USP24 degradation."

sparser
"Ubiquitination of USP7 (K869), USP24 (K421, K656) and USP36 (K338) were down-regulated."

sparser
"In addition, there was an inverse correlation between the USP24 level and the cdc20 level (an E3 ligase in the APC/C complex), but not cdh1 level, implying that APC/C cdc20 might provide the enzymatic activity for USP24 ubiquitination ( xref ; xref )."
USP24 affects autophagy
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USP24 activates autophagy.
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"Herein we found a positive correlation between targeting USP24-mediated LC3-II signaling and the cytotoxicity of Taxol in drug resistance, implying that targeting USP24-induced autophagy might be important for inhibiting drug resistance during cancer therapy (Fig. 1C)."

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"USP24, another ubiquitinating enzyme, induces autophagy in vivo and in vitro and inhibits drug resistance in cancers acquired by taxol or gefitinib treatment [14]."

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"Overall, targeting USP24 by USP24-i induces autophagy in vitro and in vivo."

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"We also investigated the molecular mechanism of how USP24-mediated autophagy in interphase and mitosis (Fig. 2, Fig. 3 and Supplementary Fig. 2)."

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"Interestingly, autolysosomes were found in mitotic cells (red arrow), indicating that USP24-mediated autophagy occurs not only in interphase but also in mitosis (Fig. 3A)."

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"The data indicated that inhibition of autophagy decreased the cell entry from G2/M (Fig. 4D(b)), and increased chromosome separation defects, micronuclei and segregation defects (Fig. 4E), indicating that autophagic induction during mitosis could maintain genomic stability.Next, the effect of targeting USP24-induced autophagy on chemotherapy- and targeted therapy-induced drug-resistant lung cancer cells was studied (Fig. 4F and Supplementary Fig. 3)."

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"Altogether, these results confirmed that USP24 promotes the autophagy-dependent ferroptosis and increases the sensitivity of HCC cells to sorafenib."

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"Next, we also used gefitinib-induced PC9 drug-resistant cells (PC9-GR) to study the effect of targeting USP24 induced autophagy on inhibiting drug resistance acquired by targeted therapy (Fig. 4F(b), Supplementary Fig. 3A, B)."

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"Gefitinib and USP24-i cocktail treatment significantly induced cell death but abolished this effect by inhibiting autophagy with Baf-A1 or CQ treatment, suggesting that targeting USP24-induced autophagy is essential for the effect of USP24-i on blocking drug resistance acquired by targeted therapy."

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"Our results confirmed that USP24 promotes autophagy-dependent ferroptosis by stabilizing Beclin1 (Fig. 8K)."
USP24 inhibits autophagy.
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"USP24 has been reported to inhibit autophagy in neurons by increasing the degradation of ULK1 to repress the human induced-pluripotent stem cell (iPSC) differentiation into dopaminergic neurons, which is involved in Parkinson’s disease (PD) [35]."

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"Furthermore, we observed elevated levels of USP24 in the substantia nigra of a subpopulation of idiopathic PD patients, suggesting that USP24 may negatively regulate autophagy in PD."

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"In a somewhat similar manner as USP14, USP24 also negatively regulates and inhibits autophagy."

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"Therefore, a decrease in USP24 during mitosis may also induce autophagy during mitosis (Fig. 3)."

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"Furthermore, our data demonstrate elevated levels of USP24 in some cases of idiopathic PD, suggesting that USP24 may negatively regulate autophagy in PD."

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"While the deubiquitinating enzyme USP20 and STAMBP/AMSH promote autophagy initiation by stabilizing ULK1 [21, 46], USP24 was found to negatively regulate autophagy and the stability of ULK1 [47]."

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"In addition, the level of p62/SQSTM1 was increased, not decreased, by USP24-i treatment, implying USP24-i targeting USP24 inhibits drug resistance might be through inducing p62/SQSTM1-mediated select autophagy-dependent ferroptosis."

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"Our study provides a novel therapeutic strategy to enhance the treatment response in sorafenib-resistant HCC.During sorafenib treatment, miR-21-5p, which is highly expressed in HCC, inhibits the ubiqu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"USP24 Negatively Regulates Autophagy through Destabilization of ULK-1."

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"Consistently, in the original screen [25] USP24 knockdown failed to upregulate autophagy in cells overexpressing BCL-2, which binds and inhibits activity of the class III PtdIns3K subunit BECN1 [34])."
USP24 affects DDB2
2 4 1 | 20 16
USP24 binds DDB2.
4 | 11 16
4 | 11 16

sparser
"Deubiquitinating protein USP24 interacts with NER pathway component DDB2."
| PMC

sparser
"The interaction between USP24 and DDB2 was confirmed by co-immunoprecipitation."

sparser
"In a yeast-two-hybrid screen, we identified that USP24 interacts with the UV damage binding protein DDB2, a subunit of the CUL4-DDB1 DDB2 ubiquitin ligase ( xref )."

sparser
"Interaction between DDB2 and USP24 was confirmed by co-precipitation."
| PMC

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"The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability."

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"These observations suggest that USP24 interacts with DDB2 in vivo, as suggested by our yeast two-hybrid screen."
USP24 activates DDB2.
1 | 4
USP24 activates DDB2. 3 / 4
1 | 2

"Usp24-mediated ddb2 deubiquitination prevents ddb2 degradation"

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"USP24 targeting DDB2 and p53."

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"Knockdown of USP24 resulted in a decreased level of DDB2, suggesting that USP24 stabilizes DDB2 by removing the ubiquitin moiety from modified DDB2, thereby preventing DDB2 degradation."
USP24 activates ubiquitinated DDB2. 2 / 2
| 2

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"To examine if USP24 can target ubiquitinated DDB2 in vitro, we treated HeLa cells expressing Hig tagged DDB2 with MG132, a proteosome inhibitor, XREF_BIBR for 1 h and exposed HeLa cells to UVC (10 J/m 2) to stimulate DDB2 uniquitination for another hour, Ni-NTA beads were then used to pulldown modified His DDB2."

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"Our group previously reported that ubiquitinated DDB2 can be targeted by USP24, and in this study, we demonstrate that USP24 is a p53 deubiquitinase, required for p53 stabilization in unstressed cells, as well as for p53 stabilization and PUMA activation after DNA damage."
USP24 increases the amount of DDB2.
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USP24 increases the amount of DDB2. 3 / 3
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"Importantly, knockdown of USP24 in two human cell lines decreased the steady-state levels of DDB2, indicating that USP24 mediated DDB2 deubiquitination prevents DDB2 degradation."
| PMC

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"As indicated in XREF_FIG, knockdown of USP24 decreased the steady-state levels of DDB2 in both HeLa and 293T cells."

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"Importantly, knockdown of USP24 in two human cell lines decreased the steady-state levels of DDB2, indicating that USP24 mediated DDB2 deubiquitination prevents DDB2 degradation."
USP24 deubiquitinates DDB2.
1 1 | 1
USP24 deubiquitinates DDB2. 3 / 3
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"<span class="match term0">USP24</span> targeting <span class="match term1">DDB2</span> and p53"

"Usp24-mediated ddb2 deubiquitination prevents ddb2 degradation"

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"DDB2 is deubiquitinated by USP24, stabilizing the entire UV-DDB-CUL4 E3 ligase from proteasomal degradation [XREF_BIBR]."
USP24 decreases the amount of DDB2.
| 1
USP24 decreases the amount of DDB2. 1 / 1
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"Knockdown of USP24 decreases steady-state level of DDB2."
DDB2 affects USP24
4 | 12 16
DDB2 binds USP24.
4 | 11 16
4 | 11 16

sparser
"Deubiquitinating protein USP24 interacts with NER pathway component DDB2."
| PMC

sparser
"The interaction between USP24 and DDB2 was confirmed by co-immunoprecipitation."

sparser
"In a yeast-two-hybrid screen, we identified that USP24 interacts with the UV damage binding protein DDB2, a subunit of the CUL4-DDB1 DDB2 ubiquitin ligase ( xref )."

sparser
"Interaction between DDB2 and USP24 was confirmed by co-precipitation."
| PMC

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"The deubiquitinating protein USP24 interacts with DDB2 and regulates DDB2 stability."

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"These observations suggest that USP24 interacts with DDB2 in vivo, as suggested by our yeast two-hybrid screen."
DDB2 activates USP24.
| 1
DDB2 activates USP24. 1 / 1
| 1

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"To investigate whether DDB2 is a direct target of USP24, we set out to evaluate interactions in vivo by co-immunoprecipitation."
USP24 affects EP300
2 1 | 20 4
USP24 binds EP300.
1 | 2 4
1 | 2 4

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"Indeed, we observed that USP24 interacts with p300, and knockdown of USP24 increased p300 ubiquitination, and this effect of USP24 knockdown was abolished after treatment with the proteasome inhibitor MG132."

sparser
"The ubiquitin hydrolase USP24 directly interacts with p300 protein, and reduces p300 protein ubiquitination and proteasome-mediated degradation, leading to p300 protein up-regulation and histone acetylation xref (Figure xref C)."

sparser
"Our previous results also indicated that USP24 can interact with p300, an acetyltransferase that contains a BRD xref , xref ."

sparser
"Thus, GFP-USP24 increased p300 protein stability, whereas knockdown USP24 decreased its stability ( xref ; xref ) and USP24 interacted with p300 ( xref )."

sparser
"Indeed, we observed that USP24 interacts with p300 (Fig.  xref ), and knockdown of USP24 increased p300 ubiquitination, and this effect of USP24 knockdown was abolished after treatment with the proteasome inhibitor MG132 (Fig.  xref )."

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"Thus, GFP-USP24 increased p300 protein stability, whereas knockdown USP24 decreased its stability (XREF_FIG; XREF_SUPPLEMENTARY) and USP24 interacted with p300 (XREF_FIG)."
USP24 decreases the amount of EP300.
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USP24 decreases the amount of EP300. 2 / 6
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"Knockdown of USP24 decreases Bax and p300 levels, and reduces Ku70 acetylation, thereby preventing cancer cell apoptosis."

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"14 In this study, we found that USP24 downregulation decreased the p300 level, thereby decreasing Ku70 acetylation."
USP24 activates EP300.
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USP24 activates EP300. 6 / 6
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"Because our previous study confirmed that p300 is a USP24 substrate in lung cancer cells 31, we wanted to confirm whether p300 is also directly regulated by USP24 in M2 macrophages."

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"In cancer cells, USP24 increased p300 and beta-TrCP, thus increasing the acetylation of histone H3 and the degradation of DNMT1 and IkappaB, resulting in the recruitment of histone H3 acetylation to the promoter region of IL-6, the reduction of DNA methylation, and the promotion of NF-kappaB nuclear translocation, thereby facilitating IL-6 expression."

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"This study shows that USP24 mediates p300, NF-kappaB, DNMT1, and beta-TrCP to regulate IL-6 expression, thus affecting cancer metastasis."

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"USP24 induces apoptosis by stabilizing p300 and Bax."

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"Thus, GFP-USP24 increased p300 protein stability, whereas knockdown USP24 decreased its stability (XREF_FIG; XREF_SUPPLEMENTARY) and USP24 interacted with p300 (XREF_FIG)."

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"USP24 induces apoptosis by enhancing the stability of p300 and BAX."
USP24 increases the amount of EP300.
1 | 4
USP24 increases the amount of EP300. 5 / 5
1 | 4

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"In M2 macrophages, USP24 increased p300 levels, subsequently enhancing the levels of NF-kappaB and IL-6."

"In this study, several cancer-related proteins (Bax, p300, E2F4 and securin) have been proven to be substrates of ubiquitin-specific peptidase 24 (USP24), and relevance has been shown between USP24 and its substrates in samples from clinical lung cancer patients. |Knockdown of USP24 decreases Bax and p300 levels"

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"Similarly, USP24 knockdown decreases p300 levels by decreasing p300 protein stability."

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"Similarly, knockdown of USP24 decreased the p300 level but did not affect its mRNA level (XREF_FIG)."

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"Up-regulated USP24 increased the protein level of β-TrCP and p300 to promote lung cancer malignancy 31."
USP24 deubiquitinates EP300.
1 | 4
USP24 deubiquitinates EP300. 5 / 5
1 | 4

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"The in vitro enzymatic assay using the purified USP24 protein demonstrated that USP24 decreased the p300 ubiquitination signal (XREF_FIG)."

"In this study, several cancer-related proteins (Bax, p300, E2F4 and securin) have been proven to be substrates of ubiquitin-specific peptidase 24 (USP24), and relevance has been shown between USP24 and its substrates in samples from clinical lung cancer patients. |Knockdown of USP24 decreases Bax and p300 levels"

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"USP24 deubiquitinates and stabilizes the histone acetyltransferase p300, thereby facilitating histone H3 acetylation of the IL-6 promoter and thus IL-6 transcription."

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"Indeed, we observed that USP24 interacts with p300, and knockdown of USP24 increased p300 ubiquitination, and this effect of USP24 knockdown was abolished after treatment with the proteasome inhibitor MG132."

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"The ubiquitin hydrolase USP24 directly engages with the p300 protein, diminishing the ubiquitination of the p300 protein and its degradation through the proteasome pathway."
USP24 inhibits EP300.
| 2
USP24 inhibits EP300. 2 / 2
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"Knockdown of USP24 also decreased the protein stability of p300 in M2 macrophages and A549 cancer cells."

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"In certain types of cancer, USP24 levels decrease during the early and mitotic stages of the cell cycle to regulate its substrates, including p300, BAX, E2F4, and Securin."
USP24 affects GSDMB
2 | 1 21 5
USP24 binds GSDMB.
2 | 7 5
2 | 7 5

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"Here, our results indicated that USP24 bound to GSDMB to stabilize GSDMB, and subsequently activated the STAT3 pathway in bladder cancer cells."

sparser
"For example, BUB1 kinase drives bladder cancer progression and proliferation by regulating STAT3 transcription (Jiang et al. xref ); in bladder cancer, USP24-GSDMB complex formation stimulates cell proliferation via STAT3-dependent mechanisms (He et al. xref ); ROC1 gives rise to bladder cancer malignancy via orchestrating NF-κB signaling activation (Wu et al. xref ); Mechanistic studies reveal that secretory granule protein II facilitates bladder cancer advancement by concurrently activating both MAPK and NF-κB pathways (Zhou et al. xref )."

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"Contrary, the interaction between ubiquitin-specific peptidase 24 (USP24) and GSDMB prevents the degradation of gasdermin B in bladder cancer, and this may be a potential therapeutic target in the future [34]."

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"In bladder cancer, the USP24/GSDMB/STAT3 axis is reported to promote tumor proliferation and growth, where USP24 interacts and stabilizes GSDMB, which in turn binds to STAT3, increases its phosphorylation and activates STAT3 signaling (30)."

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"It has been reported that USP24 binds to GSDMB to deubiquitinate and stabilize GSDMB."

sparser
"It has been reported that USP24 bound to Gasdermin B (GSDMB) via the activation of the STAT3 pathway promotes bladder cancer cell proliferation [ xref ]."

sparser
"Mechanistically, GSDMB forms a complex with USP24, which activates the STAT signaling pathway and, in turn, prevents the degradation of GSDMB, thereby sustaining its oncogenic functions in BLCA [ xref ]."

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"In addition, they confirmed that the interaction between USP24 and GSDMB can prevent degradation of GSDMB in bladder cancer cells [51]."
USP24 activates GSDMB.
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USP24 activates GSDMB. 3 / 3
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"These data suggest that USP24 promoted the stability of the GSDMB protein in bladder cancer by deubiquitinating GSDMB.Moreover, after USP24 was inhibited or overexpressed, the expression level of p-STAT3 Y705 also decreased or increased, respectively (Fig. 6I-K)."

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"This process is further enhanced by USP24, which prevents the degradation of GSDMB [166]."

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"USP24 can also prevent the degradation of GSDMB in bladder cancer cells to stabilize GSDMB to activate STAT3 phosphorylation; Enhance the growth and invasion of bladder cancer cells [ 49 ]."
USP24 bound to GSDMB activates GSDMB. 2 / 2
| 2

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"In addition, they confirmed that the interaction between USP24 and GSDMB can prevent degradation of GSDMB in bladder cancer cells [51]."

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"Contrary, the interaction between ubiquitin-specific peptidase 24 (USP24) and GSDMB prevents the degradation of gasdermin B in bladder cancer, and this may be a potential therapeutic target in the future [34]."
USP24 increases the amount of GSDMB.
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USP24 increases the amount of GSDMB. 4 / 4
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"USP24 interacts with GSDMB to up-regulate the GSDMB protein level."

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"USP24 interacts with GSDMB to up-regulate the GSDMB protein level in bladder cancer."

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"To sum up, USP24 interacts with GSDMB to up-regulate the GSDMB protein level in bladder cancer."

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"Given that USP24 promoted the expression of the GSDMB protein levels rather than that of the mRNA levels in bladder cancer cells, we hypothesized that USP24 may regulate the stability of GSDMB through the ubiquitinated proteasome pathway."
USP24 deubiquitinates GSDMB.
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USP24 deubiquitinates GSDMB. 4 / 4
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"Thus, USP24 may mediate the deubiquitination of GSDMB and maintain its stability."

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"Ubiquitin-specific proteases 24 (USP24) acted as a deubiquitinase to decrease the ubiquitination of GSDMB to stabilize it."

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"It has been reported that USP24 binds to GSDMB to deubiquitinate and stabilize GSDMB."

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"We proved that knocking down or inhibiting USP24 increased the polyubiquitination of GSDMB, but overexpressing USP24 decreased the polyubiquitination of GSDMB in bladder cancer cells (Fig. 6D, H)."
USP24 ubiquitinates GSDMB.
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USP24 ubiquitinates GSDMB. 1 / 1
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"Ubiquitin-specific proteases 24 (USP24) acted as a deubiquitinase to decrease the ubiquitination of GSDMB to stabilize it."
USP24 inhibits GSDMB.
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USP24 inhibits GSDMB. 1 / 1
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eidos
"Furthermore , we also demonstrated that USP24 interacted with GSDMB and prevented GSDMB from degradation in bladder cancer cells ."
USP24 affects TP53
1 1 | 1 21 2
USP24 deubiquitinates TP53.
1 | 8
USP24 deubiquitinates TP53. 9 / 9
1 | 8

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"These findings show that USP24 can deubiquitinate p53 in vitro."

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"Collectively, our data show that USP24 deubiquitinates p53, leading to stabilization of p53."

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"In this study, we demonstrate that USP24 deubiquitinates p53 in human cells."

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"Under both normal and stressed conditions p53 is also deubiquitinated by USP24 and thus stabilizing its level."

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"Deubiquitination of p53 by USP24 isdeubiquitination, it is possible that USP24 also acts as a tumor suppressor."

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"USP24 deubiquitinates p53 in vivo and in vitro."

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"Our data show that USP24 deubiquitinates p53 in vivo and in vitro, leading to p53 stabilization and activation."

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"They found also that USP24 deubiquitylates p53, activating the PUMA pathway, a regulator of DNA-damage-induced apoptosis."
USP24 activates TP53.
| 6 1
USP24 activates TP53. 6 / 6
| 5 1

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"There areseveral possible reasons for this : first, different DUBs might regulate the p53pathway in response to different cellu-lar stresses; second, different DUBsmight work in different cellular com-partments; and third, since p53 is suchan important protein that is ubiquiti nated by many different E3 ligases, dif-ferent DUBs may be required tocounteract p53 ubiquitination of dif-ferent ubiquitin linkage topologies.Indeed, it is known that DUBs havedifferent types of substrate cleavageactivities -- some generate free ubiqui-tin from linear substrate, some removeubiquitin from post-translationallymodified protein, and others edit polyubiquitin chains.10 A survey of the Catalog Of SomaticMutations In Cancer (COSMIC) data-base shows that USP24 is frequently mutated in various human cancers.6 Since p53 is a well-known tumor sup-pressor that regulates cell proliferationand USP24 potentiates p53 function byFigure 1."

sparser
"Moreover, we showed that the regulation of p53 activation by USP24 is not mediated by mouse double minute 2 homolog (Mdm2), its major E3 ligase."

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"Evidence that USP24 targets p53 to protect genome stability."

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"USP24 can directly regulate the activation of p53 (TP53) to facilitate DNA repair."

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"USP24 targeting DDB2 and p53."

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"It has been shown that USP24 targets p53 to regulate UV induced apoptosis."
USP24 activates ubiquitinated TP53. 1 / 1
| 1

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"These findings strongly suggest that USP24 directly targets ubiquitinated p53 and removes the ubiquitin moiety, thereby preventing p53 degradation."
USP24 inhibits TP53.
| 1 3
USP24 inhibits TP53. 4 / 5
| 1 3

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"However, some groups also demonstrated that USP24 prevents P53 from degradation to regulate the DNA damage response 23."

eidos
"However , some groups also demonstrated that USP24 prevents P53 from degradation to regulate the DNA damage response 23 ."

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"Knockdown of USP24 failed to prevent stabilization of p53 following etoposide treatment of H4 cells), suggesting that in this cell type USP24 may be dispensable for DNA damage responses or that the very low residual USP24 protein may be sufficient."

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"Depletion of USP24 significantly attenuates p53 dependent apoptosis induced by DNA damage."
USP24 binds TP53.
1 | 2 1
1 | 2 1

sparser
"Third, co-immunoprecipitation assays showed that USP24 interacts with p53."

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"This third, independent assay format thus supports a direct interaction between USP24, p53 and orf10."

reach
"Third, co-immunoprecipitation assays showed that USP24 interacts with p53."

No evidence text available
USP24 decreases the amount of TP53.
| 2
USP24 decreases the amount of TP53. 2 / 2
| 2

reach
"In the absence of MG132 treatment, USP24 depletion reduced the steady state levels of p53 (XREF_FIG, lane 1 vs 2)."

reach
"First, we depleted USP24 using several USP24specific siRNA and short hairpin RNA (shRNA) constructs and found that downregulation of USP24 decreased p53 protein levels but had no effect on p53 mRNA levels."
USP24 affects IL6
| 1 25 1
USP24 increases the amount of IL6.
| 17
USP24 increases the amount of IL6. 10 / 16
| 16

reach
"In M2 macrophages, USP24 increased p300 levels, subsequently enhancing the levels of NF-kappaB and IL-6."

reach
"Here, we provide direct evidence to support that USP24 promotes IL-6 expression, which might be beneficial for cancer therapy."

reach
"Here, we also observed that USP24 in lung cancer cells increased IL-6 expression."

reach
"USP24 increases IL-6 expression by epigenetic regulation."

reach
"Finally, we found that knockdown of DNMT1 in USP24-knockdown A549 cells can rescue IL-6 level, suggesting that USP24 mediated DNMT1 degradation is really involved in IL-6 expression."

reach
"Yu et al reported that ubiquitinase RNF126 affects the TME and promotes nasopharyngeal carcinoma progression through the regulation of PTEN ubiquitination.15 Wang et al discovered that an overexpression of USP24 in M2 macrophages can promote IL-6 transcription and tumour malignancy."

reach
"However, USP24 promotion in M2 macrophages increases IL-6 transcription by stabilizing p300 and β-TrCP [164]."

reach
"In lung cancer, USP24 up-regulates the expression of IL-6, thereby promoting the development of lung cancer."

reach
"In addition to the p300 stabilization induced by USP24 in cancer cells to regulate IL-6 expression through increased histone H3 acetylation, USP24 also reduces DNA methylation in the promoter region of IL-6 in lung cancer cells, but not in M2 macrophages, to increase IL-6 transcription by decreasing DNMT1 protein stability."

reach
"These results indicate that USP24 may upregulate IL-6 expression through directly stabilizing p300 and indirectly induce NF-kappaB expression through p300 in M2 macrophages."
Modified USP24 increases the amount of IL6. 1 / 1
| 1

reach
"While studying the role of USP24 upregulation in M2 macrophages, we found that USP24 expression in M2 macrophages enhanced cancer metastasis by inducing IL-6 expression."
USP24 activates IL6.
| 1 6
USP24 activates IL6. 7 / 7
| 1 6

reach
"USP24 induces IL-6 in tumor associated microenvironment by stabilizing p300 and beta-TrCP and promotes cancer malignancy."

reach
"Adding the physiological dose of IL-6 to the media of USP24-knockdown cells can rescue the effect of USP24 knockdown, suggesting that the USP24 mediated IL-6 in M2 macrophages and in lung cancer is an important factor that affects USP24 induced lung cancer malignancy."

reach
"To examine whether USP24 mediated IL-6 was indeed functioning in lung cancer metastasis and angiogenesis, IL-6 was added to conditioned medium derived from USP24-knockdown M2 macrophages to address malignant lung cancer cell activity."

eidos
"USP24 induces IL-6 in TME through deubiquitinating p300 and beta-TrCP , and whether WP1130 inhibiting USP24 affects IL-6 in TME needs more investigation [ 100 ] ."

reach
"USP24 induces IL-6 in TME through deubiquitinating p300 and beta-TrCP, and whether WP1130 inhibiting USP24 affects IL-6 in TME needs more investigation [XREF_BIBR]."

reach
"IL-6 upregulation by USP24 increases cancer malignancy."

reach
"USP24 promotes cancer malignancy by inducing IL-6 by stabilizing p300 and beta-transducing repeats-containing proteins (β-TrCP) to boost histone-3 acetylation and NF-B while lowering DNA (cytosine-5)-methyltransferase 1 (DNMT1) in M2 macrophages and lung cancer cells [182]."
USP24 decreases the amount of IL6.
| 2
USP24 decreases the amount of IL6. 2 / 2
| 2

reach
"Furthermore, USP24 stabilizes p300 and reduces the levels of DNMT1, thereby increasing IL-6 transcription in M2 macrophages and lung cancer cells (107)."

reach
"After analyzing the five CpG sites upstream of the transcription start site, we found that the methylation status of a CpG site located at -123 was increased in A549 cells but not in M2 macrophages after USP24 knockdown, indicating that a decrease in IL-6 methylation induced by USP24 in A549 cells, but not in M2 macrophages, increases IL-6 mRNA levels."
USP24 methylates IL6.
| 1
USP24 methylates IL6. 1 / 1
| 1

sparser
"After analyzing the five CpG sites upstream of the transcription start site, we found that the methylation status of a CpG site located at −123 was increased in A549 cells but not in M2 macrophages after USP24 knockdown, indicating that a decrease in IL-6 methylation induced by USP24 in A549 cells, but not in M2 macrophages, increases IL-6 mRNA levels."
USP24 affects HYCC1
| 23
USP24 inhibits HYCC1.
| 11
| 11

reach
"Contrary to previous findings , our results indicated that the knockdown of USP24 increased HCC proliferation in vitro, while USP24 overexpression inhibited these processes in vitro and in vivo."

reach
"It was found that overexpression of USP24 inhibited the proliferation of HCC cells, as indicated by the decrease in both cell viability (Fig. 5C) and colony formation (Fig. 5D, E)."

reach
"Previous studies have demonstrated that USP24 inhibited proliferation and migration of HCC, and our findings also indicated that USP24 increased cell death in HCC cells (Supplementary Fig. 4A)."

reach
"In a study by Cao et al., the role of USP24 in inhibiting HCC was investigated."

reach
"Knocking down USP24 promotes HCC proliferation and migration, whereas USP24 overexpression inhibits HCC in vitro and in vivo."

reach
"Therefore, overexpression of USP24 induces autophagy along with ferroptosis and reduces sorafenib resistance in HCC [34]."

reach
"This result suggested that USP24 overexpression combined with sorafenib may synergistically inhibit HCC progression."

reach
"USP24 silencing promotes HCC cell proliferation and migration in vitro."

reach
"USP24 inhibits HCC growth in mice xenograft model."

reach
"Collectively, these results suggested that the knockdown of USP24 promotes the proliferation and migration of HCC cells in vitro."
USP24 activates HYCC1.
| 11
USP24 activates HYCC1. 10 / 11
| 11

reach
"Taken together, USP24 can be employed as a promising target to restrain tumor growth and increase the efficacy of HCC immunotherapy."

reach
"The results indicated that USP24 overexpression decreased wound-healing ability (Fig. 5F and Supplementary Fig. 3A, B) and inhibited the migration of HCC cells (Fig. 5G, H)."

reach
"Depletion of USP24 significantly suppressed the proliferation of HCC cells in vitro, which could be rescued by restoration of YAP1."

reach
"Knocking down USP24 promotes HCC proliferation and migration, whereas USP24 overexpression inhibits HCC in vitro and in vivo."

reach
"We find that increased autophagy is accompanied by ferroptosis in USP24 overexpressed HCC cells and USP24 increases the susceptibility of HCC to sorafenib."

reach
"These results suggested that USP24 promotes ferroptosis of HCC cells.Taking into account that ferroptosis is a type of autophagic cell death, which we called ferritinophagy , the Fe level was measured using a colorimetric assay and a fluorescence probe."

reach
"Altogether, these results demonstrate that USP24 levels are significantly elevated in HCC and that its expression also plays an important role in predicting the clinical outcomes of patients with HCC."

reach
"The results indicated that overexpression of USP24 decreased GSH while increased the level of MDA in HCC cells (Fig. 7H, I and Supplementary Fig. 4G, H), further confirming that USP24 promotes ferroptosis in HCC cells."

reach
"To investigate whether USP24 knockdown suppressed the tumorigenesis of HCC cells in vivo, we injected Bel-7402 cells which were stably knockdown USP24 (shUSP24-2) or the negative control (shNC) into the subcutaneous of the nude mice."

reach
"USP24 markedly promoted HCC proliferation and progression in vitro and in vivo."
USP24 deubiquitinates HYCC1.
| 1
USP24 deubiquitinates HYCC1. 1 / 1
| 1

reach
"In hepatocellular carcinoma (HCC), the ubiquitin-specific peptidase 24 (USP24) deubiquitinates and stabilizes TRAF2, which, in turn, promotes cell proliferation and metastasis [107, 108]."
USP24 affects PDCD1
| 19 3
USP24 activates PDCD1.
| 9
USP24 activates PDCD1. 9 / 9
| 9

reach
"Together, our findings suggested that USP24 enhances PD-1 stability and therefore leads to T cell immunosuppression."

reach
"IL-6 increases USP24 expression to enhance PD-1 stability."

reach
"Deubiquitinase USP24 activated by IL-6/STAT3 enhances PD-1 protein stability and suppresses T cell antitumor response."

reach
"Our study demonstrated that USP24 promotes PD-1 protein stability in both CD4 and CD8 T cells and also increases levels on TAMs, suggesting USP24 as a universal regulator in PD-1 expression."

reach
"Here, we show that ubiquitin-specific peptidase 24 (USP24) promotes PD-1 protein stability by removing K48-linked polyubiquitin."

reach
"The potential of α-IL-6 for enhancing T cell function warrants further investigation.In conclusion, we reveal that IL-6/STAT3/NF-κB transcriptionally activates USP24 to drive USP24-mediated deubiquitinating machinery to stabilize PD-1 and suppress T cell activity."

reach
"USP24 promotes PD-1 protein stability, leading to T cell inactivation."

reach
"Moreover, ectopically expressing GFP-USP24 increased PD-1 membrane presentation, whereas knockdown of USP24 reduced the PD-1 level on the cell surface (Fig. 2C)."

reach
"These results prompted us to examine whether USP24 inhibition blocks PD-1/PD-L1–mediated T cell inhibition."
USP24 increases the amount of PDCD1.
| 8
USP24 increases the amount of PDCD1. 8 / 8
| 8

reach
"Furthermore, USP24 deficiency reduces PD-1 levels in CD8 + T cells and attenuates Egfr L858R -driven lung tumorigenesis in Usp24 C1695A catalytic deficient mice."

reach
"The knockdown screen of USP24 and other USPs showed that shUSP24 decreased PD-1 levels in Jurkat T cells, with shUSP47 and shUSPL1 showing a lesser effect (fig."

reach
"Notably, knockdown of USP24 (shUSP24) decreased PD-1 levels in Jurkat T cells (Fig. 2B)."

reach
"Moreover, ectopically expressing GFP-USP24 increased PD-1 membrane presentation, whereas knockdown of USP24 reduced the PD-1 level on the cell surface (Fig. 2C)."

reach
"USP24 deficiency decreased PD-1 protein expression without altering the PDCD1 mRNA level in peripheral blood mononuclear cells (PBMCs) and mouse CD8 splenic T cells (fig."

reach
"In addition, Usp24 depletion decreased PD-1 surface levels (Fig. 2I), resulting in increased OT-1 CD8 T antitumor activity (Fig. 2, J and K)."

reach
"USP24 deficiency decreased PD-1 protein expression without altering the PDCD1 mRNA level in peripheral blood mononuclear cells (PBMCs) and mouse CD8 splenic T cells (fig."

reach
"Together, IL-6 transcriptionally up-regulates PD-1 expression and stability mediated by USP24 in T cells within the TME.USP24 mRNA expression was up-regulated upon IL-6 treatment (Fig. 4G)."
USP24 binds PDCD1.
| 1 3
| 1 3

sparser
"We hypothesized that USP24 directly interacts with PD-1 to cleave ubiquitin on PD-1."

sparser
"IP-Western results further confirmed that IL-6 treatment increased interaction between USP24 and PD-1 (fig."

sparser
"S3C), indicating that USP24 binds to the intracellular region of PD-1 to facilitate deubiquitination."

reach
"IP-Western results further confirmed that IL-6 treatment increased interaction between USP24 and PD-1 (fig."
USP24 decreases the amount of PDCD1.
| 1
USP24 decreases the amount of PDCD1. 1 / 1
| 1

reach
"In addition, USP24 failed to reduce ubiquitin levels of PD-1 when its ICD was deleted (fig."
USP24 affects BAX
2 | 12 1
USP24 decreases the amount of BAX.
| 2
USP24 decreases the amount of BAX. 2 / 6
| 2

reach
"Knockdown of USP24 decreased the Bax and caspase-3 levels; conversely, these protein levels were increased in GFP-USP24-expressing cells, although no alterations were observed in the Bax mRNA level (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"Knockdown of USP24 decreases Bax and p300 levels, and reduces Ku70 acetylation, thereby preventing cancer cell apoptosis."
USP24 activates BAX.
| 5
USP24 activates BAX. 5 / 6
| 5

reach
"The localization of Bax in cells expressing GFP-USP24 indicated that USP24 expression increased Bax 's mitochondrial localization, implying that USP24 induced cell apoptosis (XREF_FIG)."

reach
"USP24 induces apoptosis by stabilizing p300 and Bax."

reach
"In addition, USP24 also directed Bax protein stability to result in cell apoptosis."

reach
"USP24 induces apoptosis by enhancing the stability of p300 and BAX."

reach
"USP24 knockdown decreases Bax protein stability in A549 and U2OS cells (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 inhibits BAX.
| 2
USP24 inhibits BAX. 1 / 3
| 1

reach
"In certain types of cancer, USP24 levels decrease during the early and mitotic stages of the cell cycle to regulate its substrates, including p300, BAX, E2F4, and Securin."
USP24 inhibits ubiquitinated BAX. 1 / 1
| 1

reach
"GFP-USP24 overexpression decreased the ubiquitinated Bax signal, and this effect was reversed by USP24 knockdown (XREF_FIG)."
USP24 increases the amount of BAX.
1 | 1
USP24 increases the amount of BAX. 2 / 2
1 | 1

reach
"MG132 treatment rescued the Bax level, indicating that USP24 increased the Bax level by enhancing protein stability, thereby contributing to cell apoptosis (XREF_SUPPLEMENTARY)."

"In this study, several cancer-related proteins (Bax, p300, E2F4 and securin) have been proven to be substrates of ubiquitin-specific peptidase 24 (USP24), and relevance has been shown between USP24 and its substrates in samples from clinical lung cancer patients. |Knockdown of USP24 decreases Bax and p300 levels"
USP24 deubiquitinates BAX.
1 | 1
USP24 deubiquitinates BAX. 2 / 2
1 | 1

reach
"In agreement with previous reports indicating that USP24 may de-ubiquitinate the pro apoptotic BH3 protein BAX [31], we also observed a decrease in BAX protein levels in USP24 knockdown cells)."

"In this study, several cancer-related proteins (Bax, p300, E2F4 and securin) have been proven to be substrates of ubiquitin-specific peptidase 24 (USP24), and relevance has been shown between USP24 and its substrates in samples from clinical lung cancer patients. |Knockdown of USP24 decreases Bax and p300 levels"
USP24 binds BAX.
| 1 1
| 1 1

sparser
"Next, we found that USP24 interacted with Bax ( xref D)."

reach
"Next, we found that USP24 interacted with Bax (XREF_FIG D)."
USP24 affects BECN1
| 9 11
USP24 binds BECN1.
| 4 11
| 4 11

sparser
"USP24 interacts with Beclin1."

sparser
"The interaction between USP24 and Beclin1 represents a key molecular mechanism that promotes autophagy related ferroptosis in HCC."

sparser
"It was found that Beclin1 could be co-immunoprecipitated by USP24 (Fig.  xref ), but no other autophagy proteins, such as ULK1 and Atg5 (Supplementary Fig.  xref ), indicating that USP24 could interact with Beclin1."

sparser
"Next, a reciprocal Co-IP assay was conducted to further confirm the interaction between USP24 and Beclin1 (Fig.  xref )."

sparser
"Additionally, cellular double immunofluorescence assay provided morphological evidence of the interaction between USP24 and Beclin1 protein (Fig.  xref and Supplementary Fig.  xref ) and the colocalization coefficients increased after USP24 overexpression (Fig.  xref and Supplementary Fig.  xref )."

sparser
"USP24 interacts endogenously with Beclin 1 and delays its degradation by reducing K48-linked ubiquitination."

sparser
"These results collectively confirmed the interaction between USP24 and Beclin1."

reach
"The endogenous interaction between USP24 and Beclin1 is confirmed."

reach
"Next, a reciprocal Co-IP assay was conducted to further confirm the interaction between USP24 and Beclin1 (Fig. 4B)."

sparser
"To the best of our knowledge, this is the first evidence demonstrating that USP24 interacts with Beclin1, primarily by inhibiting its K48-linked ubiquitination."
USP24 activates BECN1.
| 3
USP24 activates BECN1. 3 / 3
| 3

reach
"As shown in Fig. 4H–K, the overexpression of USP24 prolonged the half-life of Beclin1."

reach
"Mechanistically, USP24 prevents Beclin1 degradation by reducing K48-linked ubiquitination."

reach
"Whereby in our study, it was confirmed that USP24 increased the stability of Beclin1 by decreasing the K48-linked ubiquitination."
USP24 inhibits BECN1.
| 2
USP24 inhibits BECN1. 2 / 2
| 2

reach
"USP24 inhibits HCC progression dependent on Beclin1."

reach
"Collectively, these results demonstrated that USP24 inhibits the progression of HCC cells dependent on Beclin1."
CDK1 affects USP24
3 11 | 2 1 1
CDK1 phosphorylates USP24.
3 5 | 1 1 1
CDK1 phosphorylates USP24. 3 / 3
| 1 1 1

reach
"In this study, we found that the phosphorylation of USP24 by CDK1- or EGF mediated kinase activity decreased the USP24 level."

sparser
"In this study, we found that the phosphorylation of USP24 by CDK1- or EGF-mediated kinase activity decreased the USP24 level."

rlimsp
"In this study, we found that the phosphorylation of USP24 by CDK1- or EGF-mediated kinase activity decreased the USP24 level."
CDK1 phosphorylates USP24 on S2047. 2 / 2
1 1 |

No evidence text available

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
CDK1 phosphorylates USP24 on S2604. 2 / 2
1 1 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

No evidence text available
CDK1 phosphorylates USP24 on S1616. 2 / 2
1 1 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

No evidence text available
CDK1 bound to CCNB1 phosphorylates USP24 on S2604. 1 / 1
1 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
CDK1 bound to CCNB1 phosphorylates USP24 on S2047. 1 / 1
1 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
CDK1 decreases the amount of USP24.
6 | 1
CDK1 decreases the amount of USP24. 4 / 4
3 | 1

reach
"In this study, we found that the phosphorylation of USP24 by CDK1- or EGF mediated kinase activity decreased the USP24 level."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
CDK1 bound to CCNB1 decreases the amount of USP24. 3 / 3
3 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
WP1130 affects USP24
| 16
WP1130 inhibits USP24.
| 7
WP1130 inhibits USP24. 7 / 7
| 7

reach
"The reason we were unable to detect other DUBs that WP1130 is known to target might be due to the use of USP18 overexpressing lysates (as we observed WP1130 competitively blocks the labeling of USP9X and USP24 in HEK293T cell lysates, data not shown) and the reversible nature of modification by WP1130."

reach
"Collectively, these results demonstrate that WP1130 could inhibit T-ALL cell growth and USP24 activity in vivo."

reach
"Consistently, the inhibition of USP24 by WP1130 apparently blocked the ability of USP24 to remove ubiquitin chains from YAP1 (Fig. 3B)."

reach
"The aim of this study was to explore whether WP1130 could suppress T-ALL and the role of USP24 in T-ALL."

reach
"It should be also noted, however, that G9, as well as WP1130, was recently reported to inhibit USP24 as well as USP5 in addition to USP9X [37]."

reach
"WP1130 could inhibit the DUB activity of USP9X, USP5, USP14, USP37 and USP24 [ 84 ]."

reach
"However, we found that several reported DUB inhibitors, including WP1130 (a known inhibitor of USP5, USP9X, USP14, USP24, and UCH37, Figure 4B), inhibit USP18 protease activity in an ISG15-Rho assay (Figure S8)."
WP1130 binds USP24.
| 4
WP1130 binds USP24. 4 / 4
| 4

reach
"In order to investigate whether the WP1130 directly interacts with USP24, a cellular thermal shift assay (CETSA) was conducted [XREF_BIBR, XREF_BIBR]."

reach
"These data suggest that WP1130 directly interacts with USP24 in T-ALL cells."

reach
"WP1130 interacts with USP24 in cells."

reach
"To elucidate the interaction between WP1130 and USP24, a molecular docking study was performed."
WP1130 activates USP24.
| 3
WP1130 activates USP24. 3 / 3
| 3

reach
"In our study, using CETSA we have shown that WP1130 can direct target USP24 in T-ALL cells."

reach
"The partly specific DUB inhibitor WP1130 (Degrasyn) is able to inhibit activity of several DUBs including USP5, USP9x, USP14 and USP24 ( Kapuria et al., 2010 ; Luo et al., 2019 )."

reach
"In response to WP1130 treatment, USP24 was upregulated and acted as a compensatory mechanism for USP9x inhibition by WP1130."
WP1130 decreases the amount of USP24.
| 2
WP1130 decreases the amount of USP24. 2 / 2
| 2

reach
"However, WP1130 did not reduce the mRNA level of USP24 (Additional file 2 : Figure S2)."

reach
"However, WP1130 did not decrease the mRNA level of USP24, but directly inhibited USP24, resulting in a decrease in the level of USP24, which led to a simultaneous decrease in the level of MCL-1."
USP24 affects YAP1
| 9 7
USP24 binds YAP1.
| 5 7
| 5 7

reach
"USP24 directly interacts with and deubiquitinates YAP1, thereby stabilizing YAP1 protein levels."

reach
"To assess the molecular mechanism through which USP24 regulates YAP1 expression, we first explored the interaction between USP24 and YAP1."

reach
"An interaction between endogenous USP24 and YAP1 was also validated in Bel-7402 and Huh-7 cells (Fig. 2C)."

reach
"Owing to the absence of structural information on the interaction between USP24 and YAP1, we generated a complex structural model of the interaction between USP24 and YAP1 by using the AlphaFold3 server [36], which provided a structural view to understand the interactions between USP24 and YAP1."

reach
"The interaction between USP24 and YAP1 was also confirmed to stabilize YAP1 expression through deubiquitination and activation of the downstream target genes, such as CTGF and CYR61."

sparser
"USP24 interacts with YAP1."

sparser
"To assess the molecular mechanism through which USP24 regulates YAP1 expression, we first explored the interaction between USP24 and YAP1."

sparser
"Using a Co-IP assay, we examined the interactions between exogenous USP24 and exogenous YAP1 or between exogenous USP24 and endogenous YAP1, the results showed that USP24 and YAP1 were readily co-immunoprecipitated with each other (Fig.  xref A, B)."

sparser
"An interaction between endogenous USP24 and YAP1 was also validated in Bel-7402 and Huh-7 cells (Fig.  xref C)."

sparser
"Owing to the absence of structural information on the interaction between USP24 and YAP1, we generated a complex structural model of the interaction between USP24 and YAP1 by using the AlphaFold3 server [ xref ], which provided a structural view to understand the interactions between USP24 and YAP1."
USP24 deubiquitinates YAP1.
| 3
USP24 deubiquitinates YAP1. 3 / 3
| 3

reach
"USP24 deubiquitinates YAP1."

reach
"USP24 promotes hepatocellular carcinoma progression by deubiquitinating and stabilizing YAP1."

reach
"USP24 directly interacts with and deubiquitinates YAP1, thereby stabilizing YAP1 protein levels."
USP24 decreases the amount of YAP1.
| 1
USP24 decreases the amount of YAP1. 1 / 1
| 1

reach
"Interestingly, qRT-PCR analysis results further revealed that the downregulation of USP24 expression in HCC cells lowered the mRNA expression levels of YAP1 targeted genes, including CTGF (~ 50%; P < 0.05) and CYR61 (~ 50%; P < 0.05), without affecting the mRNA level of YAP1 (Fig. 1E), suggesting that USP24 might regulate the expression of YAP1 at the post-translational level."
USP24 affects E2F4
| 13 2
USP24 decreases the amount of E2F4.
| 4
USP24 decreases the amount of E2F4. 4 / 5
| 4

reach
"However, the overexpression of E2F4 following USP24 knockdown elevates E2F1 mRNA levels and partially reduces the G1-S transition."

reach
"USP24 inhibits the G1-S transition by increasing the E2F4 level."

reach
"Knockdown of USP24 decreased the E2F4, p130 and TFDP1 levels, which form a complex to regulate the G1-S transition, but did not alter the E2F4 and TDFP1 mRNA levels (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"Targeting USP24 decreases E2F4 and TRAF6 levels to induce autophagy in interphase and mitotic cancer cells."
USP24 binds E2F4.
| 2 2
| 2 2

reach
"Further study indicated that USP24 could interact with E2F4 (XREF_FIG)."

reach
"Further studies have demonstrated the interaction between USP24 and E2F4."

sparser
"Further studies have demonstrated the interaction between USP24 and E2F4."

sparser
"Further study indicated that USP24 could interact with E2F4 ( xref )."
USP24 inhibits E2F4.
| 3
USP24 inhibits E2F4. 3 / 3
| 3

reach
"In certain types of cancer, USP24 levels decrease during the early and mitotic stages of the cell cycle to regulate its substrates, including p300, BAX, E2F4, and Securin."

reach
"In this study, we found that targeting USP24 by the specific USP24 inhibitors, USP24-i and its analogues, dramatically activated autophagy in the interphase and mitotic periods of lung cancer cells by inhibiting E2F4 and TRAF6, respectively."

reach
"Knockdown of USP24 decreased E2F4 protein stability (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 activates E2F4.
| 3
USP24 activates E2F4. 3 / 3
| 3

reach
"However, we found that the ULK and LC3 gene expression was regulated by USP24-mediated E2F4 and E2F1 in interphase, and beclin-1 levels were increased by a decrease in TRAF6 levels in mitosis."

reach
"Taken together, our results demonstrated that USP24 knockdown decreased E2F4, p130 and TFDP1, resulting in an increase in the E2F1 levels and a subsequent increase in the G1-S transition, leading to tumorigenesis."

reach
"Further experiments reveal that USP24 knockdown decreases the stability of E2F4 protein, p130, and TFDP1, resulting in an increase in E2F1 levels and a promotion of the G1-S transition, ultimately lea[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 deubiquitinates E2F4.
| 1
USP24 leads to the deubiquitination of E2F4. 1 / 1
| 1

reach
"Conversely, knockdown of USP24 in A549 cells increased the E2F4 ubiquitination signal (XREF_FIG)."

reach
"Contrary to previous findings , our results indicated that the knockdown of USP24 increased HCC proliferation in vitro, while USP24 overexpression inhibited these processes in vitro and in vivo."

reach
"It was found that overexpression of USP24 inhibited the proliferation of HCC cells, as indicated by the decrease in both cell viability (Fig. 5C) and colony formation (Fig. 5D, E)."

reach
"Previous studies have demonstrated that USP24 inhibited proliferation and migration of HCC, and our findings also indicated that USP24 increased cell death in HCC cells (Supplementary Fig. 4A)."

reach
"The results of the EdU and Transwell assays showed that the addition of the miR-21-5p inhibitor significantly inhibited cell proliferation and migration, that USP24 overexpression could reverse the ef[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"USP24 silencing promotes HCC cell proliferation and migration in vitro."

trips
"Silencing USP24 increases the cancer formation by inhibiting cellular apoptosis and increasing cellular proliferation."

reach
"For instance, although USP24 inhibits the proliferation of tumor cells, it promotes the spread of metastases in other types of malignancies [4, 35]."

reach
"Collectively, these results suggested that the knockdown of USP24 promotes the proliferation and migration of HCC cells in vitro."

reach
"It was found that overexpressed USP24 promoted GC cell proliferation and abnormal glycolysis."

reach
"Depletion of USP24 significantly suppressed the proliferation of HCC cells in vitro, which could be rescued by restoration of YAP1."

reach
"Furthermore, knockdown of USP24 markedly inhibits the proliferation of Jurkat cells."

reach
"As depicted in Fig. 6A, B, knockdown of USP24 significantly suppressed cell proliferation by approximately 50% in Bel-7402 cells (P < 0.01), 33% in Huh-7 cells (P < 0.01) and 59% in PLC/PRF/5 cells (P < 0.001)."

reach
"USP24 markedly promoted HCC proliferation and progression in vitro and in vivo."

reach
"In addition, we also found that USP24 promoted cell proliferation and tumor growth of HCC in vitro and in vivo."
USP24 bound to GSDMB activates cell population proliferation. 1 / 1
| 1

reach
"USP24-GSDMB complex promotes bladder cancer proliferation via activation of the STAT3 pathway."
BECN1 affects USP24
| 4 11
| 4 11

sparser
"USP24 interacts with Beclin1."

sparser
"The interaction between USP24 and Beclin1 represents a key molecular mechanism that promotes autophagy related ferroptosis in HCC."

sparser
"It was found that Beclin1 could be co-immunoprecipitated by USP24 (Fig.  xref ), but no other autophagy proteins, such as ULK1 and Atg5 (Supplementary Fig.  xref ), indicating that USP24 could interact with Beclin1."

sparser
"Next, a reciprocal Co-IP assay was conducted to further confirm the interaction between USP24 and Beclin1 (Fig.  xref )."

sparser
"Additionally, cellular double immunofluorescence assay provided morphological evidence of the interaction between USP24 and Beclin1 protein (Fig.  xref and Supplementary Fig.  xref ) and the colocalization coefficients increased after USP24 overexpression (Fig.  xref and Supplementary Fig.  xref )."

sparser
"USP24 interacts endogenously with Beclin 1 and delays its degradation by reducing K48-linked ubiquitination."

sparser
"These results collectively confirmed the interaction between USP24 and Beclin1."

reach
"The endogenous interaction between USP24 and Beclin1 is confirmed."

reach
"Next, a reciprocal Co-IP assay was conducted to further confirm the interaction between USP24 and Beclin1 (Fig. 4B)."

sparser
"To the best of our knowledge, this is the first evidence demonstrating that USP24 interacts with Beclin1, primarily by inhibiting its K48-linked ubiquitination."
GSDMB affects USP24
2 | 7 5
2 | 7 5

reach
"Here, our results indicated that USP24 bound to GSDMB to stabilize GSDMB, and subsequently activated the STAT3 pathway in bladder cancer cells."

sparser
"For example, BUB1 kinase drives bladder cancer progression and proliferation by regulating STAT3 transcription (Jiang et al. xref ); in bladder cancer, USP24-GSDMB complex formation stimulates cell proliferation via STAT3-dependent mechanisms (He et al. xref ); ROC1 gives rise to bladder cancer malignancy via orchestrating NF-κB signaling activation (Wu et al. xref ); Mechanistic studies reveal that secretory granule protein II facilitates bladder cancer advancement by concurrently activating both MAPK and NF-κB pathways (Zhou et al. xref )."

reach
"Contrary, the interaction between ubiquitin-specific peptidase 24 (USP24) and GSDMB prevents the degradation of gasdermin B in bladder cancer, and this may be a potential therapeutic target in the future [34]."

No evidence text available

reach
"In bladder cancer, the USP24/GSDMB/STAT3 axis is reported to promote tumor proliferation and growth, where USP24 interacts and stabilizes GSDMB, which in turn binds to STAT3, increases its phosphorylation and activates STAT3 signaling (30)."

No evidence text available

reach
"It has been reported that USP24 binds to GSDMB to deubiquitinate and stabilize GSDMB."

sparser
"It has been reported that USP24 bound to Gasdermin B (GSDMB) via the activation of the STAT3 pathway promotes bladder cancer cell proliferation [ xref ]."

sparser
"Mechanistically, GSDMB forms a complex with USP24, which activates the STAT signaling pathway and, in turn, prevents the degradation of GSDMB, thereby sustaining its oncogenic functions in BLCA [ xref ]."

reach
"In addition, they confirmed that the interaction between USP24 and GSDMB can prevent degradation of GSDMB in bladder cancer cells [51]."
USP24 affects PTTG1
| 9 2
USP24 increases the amount of PTTG1.
| 3
USP24 increases the amount of PTTG1. 2 / 2
| 2

reach
"Knocking down USP24 increased the ubiquitination signal of Securin and decreased its expression, while overexpression of USP24 increased Securin expression."

reach
"Taken together, our results suggested that USP24 was decreased by APC/C cdc20 to decrease the securin level, which was beneficial for the metaphase-anaphase transition and enhanced cell cycle progression."
Modified USP24 increases the amount of PTTG1. 1 / 1
| 1

reach
"Knockdown of USP24 decreased securin expression and increased the securin ubiquitination signal, whereas USP24 overexpression increased securin expression (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 decreases the amount of PTTG1.
| 1
USP24 decreases the amount of PTTG1. 1 / 3
| 1

reach
"Knockdown of USP24 decreased securin expression and increased the securin ubiquitination signal, whereas USP24 overexpression increased securin expression (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 binds PTTG1.
| 1 2
| 1 2

sparser
"It was discovered that USP24 interacts with Securin."

reach
"Here we found that USP24 interacted with securin in mitotic cells (XREF_FIG; XREF_SUPPLEMENTARY)."

sparser
"Here we found that USP24 interacted with securin in mitotic cells ( xref ; xref )."
USP24 inhibits PTTG1.
| 2
USP24 inhibits PTTG1. 2 / 2
| 2

reach
"In certain types of cancer, USP24 levels decrease during the early and mitotic stages of the cell cycle to regulate its substrates, including p300, BAX, E2F4, and Securin."

reach
"In summary, targeting USP24 by USP24-i inhibits E2F4, thereby increasing E2F1 expression, and subsequently increasing ULK1 and LC3 expression, resulting in autophagy in interphase.In previous studies, we showed that USP24 is downregulated during mitosis to destabilize securin, which will be beneficial for mitotic progression [32]."
USP24 ubiquitinates PTTG1.
| 1
USP24 leads to the ubiquitination of PTTG1. 1 / 1
| 1

reach
"Knockdown of USP24 decreased securin expression and increased the securin ubiquitination signal, whereas USP24 overexpression increased securin expression (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 deubiquitinates PTTG1.
| 1
USP24 leads to the deubiquitination of PTTG1. 1 / 1
| 1

reach
"Knockdown of USP24 decreased securin expression and increased the securin ubiquitination signal, whereas USP24 overexpression increased securin expression (XREF_FIG; XREF_SUPPLEMENTARY)."
Bisphenol A affects USP24
12 |
Bisphenol A increases the amount of USP24.
6 |
Bisphenol A increases the amount of USP24. 6 / 6
6 |

No evidence text available

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Bisphenol A decreases the amount of USP24.
5 |
Bisphenol A decreases the amount of USP24. 5 / 5
5 |

No evidence text available

No evidence text available

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Bisphenol A demethylates USP24.
1 |
Bisphenol A demethylates USP24. 1 / 1
1 |

No evidence text available
YAP1 affects USP24
| 5 7
| 5 7

reach
"USP24 directly interacts with and deubiquitinates YAP1, thereby stabilizing YAP1 protein levels."

reach
"To assess the molecular mechanism through which USP24 regulates YAP1 expression, we first explored the interaction between USP24 and YAP1."

reach
"An interaction between endogenous USP24 and YAP1 was also validated in Bel-7402 and Huh-7 cells (Fig. 2C)."

reach
"Owing to the absence of structural information on the interaction between USP24 and YAP1, we generated a complex structural model of the interaction between USP24 and YAP1 by using the AlphaFold3 server [36], which provided a structural view to understand the interactions between USP24 and YAP1."

reach
"The interaction between USP24 and YAP1 was also confirmed to stabilize YAP1 expression through deubiquitination and activation of the downstream target genes, such as CTGF and CYR61."

sparser
"USP24 interacts with YAP1."

sparser
"To assess the molecular mechanism through which USP24 regulates YAP1 expression, we first explored the interaction between USP24 and YAP1."

sparser
"Using a Co-IP assay, we examined the interactions between exogenous USP24 and exogenous YAP1 or between exogenous USP24 and endogenous YAP1, the results showed that USP24 and YAP1 were readily co-immunoprecipitated with each other (Fig.  xref A, B)."

sparser
"An interaction between endogenous USP24 and YAP1 was also validated in Bel-7402 and Huh-7 cells (Fig.  xref C)."

sparser
"Owing to the absence of structural information on the interaction between USP24 and YAP1, we generated a complex structural model of the interaction between USP24 and YAP1 by using the AlphaFold3 server [ xref ], which provided a structural view to understand the interactions between USP24 and YAP1."

reach
"For example, USP24 positively regulates drug resistance partially by stabilizing ABC transporters, including ABCB1, ABCG1, and ABCC1, to pump out drugs from cancer cells [61]."

reach
"One study indicated that USP24 stabilized permeability glycoprotein (P-gp) and ATP binding cassette subfamily G member 2 (ABCG2) in a proteosome- and lysosome-dependent manner, respectively [3], implying that lysosomal function may be involved in USP24-mediated drug resistance."

reach
"All the data indicated that USP24 may positively regulated drug resistance acquired by gefitinib treatment."

reach
"The detailed cell death signal pathway(s) triggered by targeting USP24 need to be clarified in the future.In addition to the gene expression involved in lung cancer progression, other genes related to other pathways involved in hematopoietic disease, dermatomyositis, leukocyte migration/cell-cell adhesion/chemotaxis and fat cell differentiation might also be related to USP24-mediated drug resistance."

reach
"Studies have found that USP24 is linked to the progression of lung cancer and can accelerate the occurrence of drug resistance ."

reach
"The data indicated that the tumor area in the gefitinib-induced EGFR *USP24 drug-resistant mice was inhibited compared with that in the gefitinib-induced EGFR *USP24 drug-resistant mice, indicating that USP24 promotes drug resistance acquired during cancer therapy (Fig. 5C, upper panel)."

reach
"Our previous studies indicate that USP24 promotes drug resistance acquired through cancer therapy by stabilizing ABC transporters and inducing the genomic instability [3]."

reach
"USP24, another ubiquitinating enzyme, induces autophagy in vivo and in vitro and inhibits drug resistance in cancers acquired by taxol or gefitinib treatment [14]."

reach
"In addition, rapamycin-induced autophagy was also essential for the effect of USP24 in inhibiting the drug resistance to Taxol and gefitinib (Supplementary Fig. 3C, D)."

reach
"USP24 functional knockout inhibits drug resistance in drug resistant mice with gefitinib-induced EGFR L858R lung cancer."

reach
"In summary, an increase in the expression of USP24 in cancer cells is beneficial for the induction of drug resistance and targeting USP24 by USP24-i-101 optimized from USP24-i inhibits drug resistance acquired during cancer therapy by increasing PD-L1 protein degradation and genomic stability in an autophagy induction-dependent manner."

reach
"Targeting USP24 by USP24-i-101 inhibits drug resistance in vivo."
USP24 affects TBK1
1 | 4 7
USP24 binds TBK1.
1 | 7
1 | 7

No evidence text available

sparser
"Our above studies have demonstrated that USP24 interacts with TBK1."

sparser
"We found that USP24 could interact with TBK1, but not RIG-I, MAVS, TRAF6/3, or IRF3 ( xref A–F)."

sparser
"Furthermore, we confirmed that endogenous TBK1 could interact with endogenous USP24 in HEK293T cells ( xref G)."

sparser
"USP24 interacts with TBK1 to reduce the K63-linked polyubiquitination of TBK1, thereby affecting IRF3 activation and IFN-I production [ xref ]."

sparser
"Further studies revealed that USP24 physically interacted with TBK1, and can reduce K63-linked polyubiquitination of TBK1."

sparser
"Thus, this study revealed the regulation of the USP24-TBK1 axis on EV71 infection."

sparser
"USP24 can interact with TBK1."
USP24 inhibits TBK1.
| 2
USP24 inhibits TBK1. 2 / 2
| 2

reach
"Knockdown of USP24 upregulated TBK1 K63-linked polyubiquitination, promoted the phosphorylation and nuclear translocation of IRF3, and in turn improved IFN-I production during EV71 infection."

reach
"Mechanistically, USP24 can target TBK1 and reduce TBK1 K63-linked polyubiquitination modifications dependently on the Lys154 residue of TBK1, resulting in decreased IRF3 activation and nuclear translocation."
USP24 deubiquitinates TBK1.
| 2
USP24 leads to the deubiquitination of TBK1. 2 / 2
| 2

reach
"Collectively, these findings suggested that USP24 could target TBK1 to regulate IRF3 activation and IFN-I production.3.6 USP24 reduces K63-linked polyubiquitination of TBK1."

reach
"Here, we found that USP24 can downregulate TBK1 ubiquitination and influence IRF3 activation during EV71 infection."
USP24 affects MCL1
| 9 3
USP24 deubiquitinates MCL1.
| 5
USP24 deubiquitinates MCL1. 5 / 5
| 5

reach
"In T-ALL, USP24 exhibits a negative association with the prognosis of T-ALL and stabilizes Mcl-1 protein by deubiquitylating MCL-1 via the USP24-MCL-1 axis."

reach
"On the other hand, USP24 can deubiquitinate MCL-1 to prevent its degradation via K48 ubiquitin protein, thereby stabilizing MCL-1 and maintaining the mitochondrial transmembrane potential of tumor cel[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In myeloma, USP24 may play a major role in deubiquitination of MCL-1."

reach
"Inhibition of USP9X and USP24 increases the ubiquitination of MCL-1 and decreases the level of MCL-1."

reach
"In acute lymphocyte leukemia, USP24 deubiquitinates Mcl-1 and promotes T-ALL cell survival, as depicted in Fig. 4 below.Gasdermin B (GSDMB) has been shown to promote the progression of bladder cancer [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 binds MCL1.
| 1 3
| 1 3

reach
"It has been observed that both USP9X and USP24 can bind to MCL-1 and enhance its stability."

sparser
"The mechanism is that USP24-MCL-1 axis accelerates the breakdown of mitochondrial transmembrane potential and induces apoptosis."

sparser
"Our laboratory’s previous study reported that USP24 plays an important role in the survival of T-cell acute lymphoblastic leukemia (T-ALL) cells and targeting USP24-Mcl-1 axis may represent a novel strategy in the treatment of T-ALL [ xref ]."

sparser
"In T-ALL, USP24 exhibits a negative association with the prognosis of T-ALL and stabilizes Mcl-1 protein by deubiquitylating MCL-1 via the USP24-MCL-1 axis."
USP24 increases the amount of MCL1.
| 3
USP24 increases the amount of MCL1. 3 / 3
| 3

reach
"Thus, EOAI3402143, an inhibitor of the deubiquitinases Usp9x and Usp24, reduced Mcl-1 protein levels in MM cells and lowered disease burden in an animal model of MM [98]."

reach
"However, WP1130 did not decrease the mRNA level of USP24, but directly inhibited USP24, resulting in a decrease in the level of USP24, which led to a simultaneous decrease in the level of MCL-1."

reach
"Inhibition of USP9X and USP24 increases the ubiquitination of MCL-1 and decreases the level of MCL-1."
TRX1 affects USP24
| 6 5
TRX1 binds USP24.
| 4 5
| 4 5

reach
"HCMV pUL38 directly binds to USP24, and these interactions are essential for HCMV pUL38 dependent inhibition of apoptosis [XREF_BIBR]."

reach
"Overall, these results support the hypothesis that pUL38 binds to USP24 to reduce ferritinophagy, which may then protect cells from lysosome dysfunction induced cell death."

sparser
"Mutant pUL38 TFV/AAA protein did not bind to USP24 and failed to prevent cell death induced by pUL38-deficient HCMV infection."

reach
"PUL38 binds to ubiquitin specific protease 24 (USP24), a protein that mediates autophagic ferritin degradation in lysosomes."

sparser
"HCMV pUL38 can interact with host deubiquitinase USP24 which can stabilize ferritinophagy receptor NCOA4."

sparser
"Overall, these results support the hypothesis that pUL38 binds to USP24 to reduce ferritinophagy, which may then protect cells from lysosome dysfunction induced cell death."

sparser
"USP24 regulated and stabilized the protein levels of NCOA4 thereby promoting ferritinophagy during HCMV infection, while the binding of USP24 to pUL38 was able to reduce ferritinophagy to promote iron-dependent ER stress-induced cell death ( xref ; xref )."

reach
"USP24 regulated and stabilized the protein levels of NCOA4 thereby promoting ferritinophagy during HCMV infection, while the binding of USP24 to pUL38 was able to reduce ferritinophagy to promote iron-dependent ER stress-induced cell death (Santana-Codina and Mancias, 2018; Sun et al., 2018)."

sparser
"HCMV pUL38 directly binds to USP24, and these interactions are essential for HCMV pUL38-dependent inhibition of apoptosis [ xref ]."
TRX1 inhibits USP24.
| 2
TRX1 inhibits USP24. 2 / 2
| 2

reach
"This shows that pUL38 antagonizes USP24 to reduce ferritinophagy and increase cell viability and successful virus infection."

reach
"By interacting with USP24, pUL38 can restrict the functions of USP24 and inhibit ferroptosis, thus promoting the proliferation of HCMV (Sun et al., 2018)."
| 1 9
| 6

reach
"Down-regulation of USP24 but not USP9X induces growth inhibition and apoptosis of T-ALL cells."

reach
"These results indicate that USP24 promotes UV induced apoptosis in HCT116 cells, consistent with the notion that p53 directed apoptosis is attenuated in USP24 depleted cells which lack robust p53 stabilization after UV treatment."

reach
"Overexpression of USP24 stabilizes Securin and Bax to induce apoptosis [37]."

reach
"Using this deactivated CRISP-Cas9-SAM system we successfully reactivated USP24 and demonstrate that endogenously induced USP24 could partially rescue the apoptosis induced by WP1130 in Jurkat cells."

reach
"USP24 induces apoptosis by stabilizing p300 and Bax."

reach
"USP24 induces apoptosis by enhancing the stability of p300 and BAX."
| 1 3

reach
"Furthermore, we demonstrated that knockdown of USP24 but not USP9X could significantly induce growth inhibition and apoptosis of T-ALL cells."

reach
"USP24 depletion inhibits apoptosis in response to UV damage."

trips
"Silencing USP24 increases the cancer formation by inhibiting cellular apoptosis and increasing cellular proliferation."

reach
"Depletion of USP24 significantly attenuates p53 dependent apoptosis induced by DNA damage."
USP24 affects ULK1
1 | 9
USP24 deubiquitinates ULK1.
1 | 3
USP24 leads to the deubiquitination of ULK1. 4 / 4
1 | 3

reach
"Our data indicate that USP24 knockdown leads to increase in ULK1 ubiquitination, increased ULK1 protein stability and kinase activity."

reach
"These data suggest that USP24 normally negatively regulates ubiquitination and stability of ULK1, therefore interrupting the positive feed-back amplifying induction of autophagy."

"Our data indicate that <span class="match term0">USP24</span> regulates autophagy by affecting ubiquitination and stability of the <span class="match term1">ULK1</span> protein"

reach
"We detected increased levels of ULK1 ubiquitination following USP24 knockdown as compared to nt controls)."
USP24 inhibits ULK1.
| 3
USP24 inhibits ULK1. 3 / 3
| 3

reach
"USP24 has been reported to inhibit autophagy in neurons by increasing the degradation of ULK1 to repress the human induced-pluripotent stem cell (iPSC) differentiation into dopaminergic neurons, which is involved in Parkinson’s disease (PD) [35]."

reach
"While the deubiquitinating enzyme USP20 and STAMBP/AMSH promote autophagy initiation by stabilizing ULK1 [21, 46], USP24 was found to negatively regulate autophagy and the stability of ULK1 [47]."

reach
"USP24 knockdown decreased degradation of the ULK1 protein in the presence of protein synthesis inhibitor, cycloheximide)."
USP24 decreases the amount of ULK1.
| 3
USP24 decreases the amount of ULK1. 3 / 3
| 3

reach
"Real time qPCR data revealed no significant change in ULK1 mRNA levels following knockdown of USP24."

reach
"These data indicate that the observed increase in ULK1 protein levels following USP24 knockdown is not due to transcriptional upregulation."

reach
"USP24 negatively regulated the expression level and activity of ULK-1 through deubiquitination, where USP24 KD was shown to increase the stability and decrease the degradation of ULK-1 without affecting the complex at the transcriptional level."
USP24 affects Neoplasms
| 9
USP24 activates Neoplasms.
| 5
| 5

reach
"In addition, we also found that USP24 promoted cell proliferation and tumor growth of HCC in vitro and in vivo."

reach
"The USP24-specific inhibitor, USP24-i-101 alone or in combination with immunotherapy anti-CTLA4, profoundly reactivates T cell functions in the TME and markedly induces tumor inhibition."

reach
"The results indicated that USP24 knockdown significantly decreased tumor volume by 67% (P < 0.001) and reduced tumor weight by 43% (P < 0.001) relative to the shNC control group (Fig. 6H, I)."

reach
"Ubiquitin-specific peptidase 24 accelerates aerobic glycolysis and tumor progression in gastric carcinoma through stabilizing PLK1 to activate NOTCH1."

reach
"A recent publication found that USP24 accelerates aerobic glycolysis and tumor progression in gastric carcinoma through stabilizing PLK1 to activate NOTCH1 [28]."
USP24 inhibits Neoplasms.
| 4
| 4

reach
"Deletion of USP24 enhanced the tumor-killing ability of CD8 + T cells."

reach
"After 10 weeks of doxycycline induction, mice expressing Egfr /Usp24 had reduced tumor nodules and areas compared to Egfr /Usp24 mice (Fig. 1, B and C, and fig."

reach
"It was demonstrated that USP24 overexpression inhibited tumor growth compared with the control group (Fig. 8B–E)."

reach
"Collectively, these results indicated that USP24 inhibits tumor growth in vivo, further supporting its role as a potential therapeutic target for HCC."
USP24 affects ferroptosis
| 9
| 9

reach
"USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1."

reach
"Interestingly, we found that ferroptosis induced by USP24 is dependent on autophagy."

reach
"Consistent with a previous study discovered that USP24 regulated ferritinophagy , our results demonstrated that USP24 regulated iron metabolism thus promoting ferroptosis, and increased HCC sensitivity to sorafenib."

reach
"These findings show that USP24 upregulates NF-κB to promote ferroptosis in DCM."

reach
"These results suggested that USP24 promotes ferroptosis of HCC cells.Taking into account that ferroptosis is a type of autophagic cell death, which we called ferritinophagy , the Fe level was measured using a colorimetric assay and a fluorescence probe."

reach
"It has been shown that USP24 promotes ferroptosis by activating NF-κB in DiaCM model [214]."

reach
"The results indicated that overexpression of USP24 decreased GSH while increased the level of MDA in HCC cells (Fig. 7H, I and Supplementary Fig. 4G, H), further confirming that USP24 promotes ferroptosis in HCC cells."

reach
"Altogether, these results confirmed that USP24 promotes the autophagy-dependent ferroptosis and increases the sensitivity of HCC cells to sorafenib."

reach
"Our results confirmed that USP24 promotes autophagy-dependent ferroptosis by stabilizing Beclin1 (Fig. 8K)."
USP24 affects TRX1
| 4 5
| 4 5

reach
"HCMV pUL38 directly binds to USP24, and these interactions are essential for HCMV pUL38 dependent inhibition of apoptosis [XREF_BIBR]."

reach
"Overall, these results support the hypothesis that pUL38 binds to USP24 to reduce ferritinophagy, which may then protect cells from lysosome dysfunction induced cell death."

sparser
"Mutant pUL38 TFV/AAA protein did not bind to USP24 and failed to prevent cell death induced by pUL38-deficient HCMV infection."

reach
"PUL38 binds to ubiquitin specific protease 24 (USP24), a protein that mediates autophagic ferritin degradation in lysosomes."

sparser
"HCMV pUL38 can interact with host deubiquitinase USP24 which can stabilize ferritinophagy receptor NCOA4."

sparser
"Overall, these results support the hypothesis that pUL38 binds to USP24 to reduce ferritinophagy, which may then protect cells from lysosome dysfunction induced cell death."

sparser
"USP24 regulated and stabilized the protein levels of NCOA4 thereby promoting ferritinophagy during HCMV infection, while the binding of USP24 to pUL38 was able to reduce ferritinophagy to promote iron-dependent ER stress-induced cell death ( xref ; xref )."

reach
"USP24 regulated and stabilized the protein levels of NCOA4 thereby promoting ferritinophagy during HCMV infection, while the binding of USP24 to pUL38 was able to reduce ferritinophagy to promote iron-dependent ER stress-induced cell death (Santana-Codina and Mancias, 2018; Sun et al., 2018)."

sparser
"HCMV pUL38 directly binds to USP24, and these interactions are essential for HCMV pUL38-dependent inhibition of apoptosis [ xref ]."
USP24 affects sorafenib
| 8
USP24 activates sorafenib.
| 7
| 7

reach
"Consistent with a previous study discovered that USP24 regulated ferritinophagy , our results demonstrated that USP24 regulated iron metabolism thus promoting ferroptosis, and increased HCC sensitivity to sorafenib."

reach
"More interestingly, the upregulation of autophagy promoted the occurrence of autophagy-dependent ferroptosis in HCC, and USP24 could also increase the sensitivity of HCC to sorafenib."

reach
"USP24 promotes ferritinophagy and increases HCC sensitivity to sorafenib."

reach
"We find that increased autophagy is accompanied by ferroptosis in USP24 overexpressed HCC cells and USP24 increases the susceptibility of HCC to sorafenib."

reach
"To determine whether USP24 could increase the sensitivity of HCC cells to sorafenib, HCC cells were treated with different doses of sorafenib."

reach
"In above results, knockdown of USP24 can inhibit progress of sorafenib resistance in HCC."

reach
"Altogether, these results confirmed that USP24 promotes the autophagy-dependent ferroptosis and increases the sensitivity of HCC cells to sorafenib."
USP24 inhibits sorafenib.
| 1
| 1

reach
"Therefore, overexpression of USP24 induces autophagy along with ferroptosis and reduces sorafenib resistance in HCC [34]."
USP24 affects XRCC6
| 8
USP24 acetylates XRCC6.
| 4
USP24 leads to the acetylation of XRCC6. 3 / 3
| 3

reach
"Knockdown of USP24 decreases Bax and p300 levels, and reduces Ku70 acetylation, thereby preventing cancer cell apoptosis."

reach
"14 In this study, we found that USP24 downregulation decreased the p300 level, thereby decreasing Ku70 acetylation."

reach
"Overall, USP24 induces apoptosis by stabilizing BAX and p300, increasing the acetylation of Ku70, and promoting BAX mitochondrial localization [ 54 ]."
Modified USP24 leads to the acetylation of XRCC6. 1 / 1
| 1

reach
"Overexpression or knockdown of USP24 did not affect the Ku70 level but increased or decreased Ku70 acetylation, respectively (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 binds XRCC6.
| 3
| 3

reach
"The data revealed that USP24 interacted with Ku70 (XREF_FIG)."

reach
"15 Herein, we used a yeast two-hybrid assay to study the interaction between Ku70 and USP24 (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"XREF_BIBR, XREF_BIBR Our preliminary results from a yeast two-hybrid assay showed that Ku70 interacts with USP24; however, the role of USP24 in Bax mediated apoptosis is unknown."
USP24 deacetylates XRCC6.
| 1
USP24 leads to the deacetylation of XRCC6. 1 / 1
| 1

reach
"Knockdown of USP24 decreases Bax and p300 levels, and reduces Ku70 acetylation, thereby preventing cancer cell apoptosis."
USP24 affects SIRT7
2 | 4 2
USP24 binds SIRT7.
2 | 2
2 | 2

No evidence text available

No evidence text available

sparser
"miR-21-5p promoted the expression of ubiquitination-specific peptidase 24 (USP24); the interaction between USP24 and SIRT7 decreased the level of SIRT7 ubiquitination in HCC cells, while upregulating the LC3-II/I ratio and beclin-1 level."

sparser
"Our results demonstrated that USP24 interacts with SIRT7, and that overexpression of USP24 significantly reduced SIRT7 polyubiquitination."
USP24 deubiquitinates SIRT7.
| 2
USP24 deubiquitinates SIRT7. 2 / 2
| 2

reach
"Our results demonstrated that USP24 interacts with SIRT7, and that overexpression of USP24 significantly reduced SIRT7 polyubiquitination."

reach
"These results indicated that USP24 can deubiquitinate SIRT7 in HCC.Based on the above studies, we further investigated the role of miR-21-5p, USP24 and SIRT7 in the mechanism of sorafenib resistance i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 decreases the amount of SIRT7.
| 2
USP24 decreases the amount of SIRT7. 2 / 2
| 2

reach
"By examining the ubiquitination level of SIRT7 in cells after overexpression of USP24, we discovered that excessive USP24 expression markedly decreased the polyubiquitination level of SIRT7 ( Fig. 4 D[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"First, we examined the ubiquitination level of SIRT7, and the results showed that USP24 overexpression prominently reduced the ubiquitination level of SIRT7 compared with miR-21-5p inhibitor treatment[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 affects RUNX2
3 | 3 2
USP24 binds RUNX2.
3 | 1 2
3 | 1 2

No evidence text available

No evidence text available

No evidence text available

sparser
"Mutation of the serine-28 site of Runx2 to aspartate (D) to mimic Runx2 phosphorylation revealed that USP24 directly interacted with Runx2 S28D rather than Runx2 in NP-1 cells co-expressing Flag Runx2 S28D + MYC USP24 (Fig.  xref E)."

sparser
"Nevertheless, screening inhibitors that target p300-Runx2 S28D and Runx2 S28D -USP24 interactions appears to be a promising strategy for specifically decreasing ADAMTS expression."

reach
"To examine endogenous interaction between RUNX2 and CK2 or USP24, cell lysates were obtained from mouse COBs and prepared in lysis buffer (50mM Tris-HCl (pH 7.4), 150mM NaCl, 1% Triton X-100, 1mM EDTA, 1mM EGTA, 50mM NaF, 1mM Na 3 VO 4, 1mM PMSF, and protease inhibitor cocktail (Sigma))."
USP24 phosphorylates RUNX2.
| 1
USP24 phosphorylates RUNX2. 1 / 1
| 1

reach
"We also found that ubiquitin-specific peptidase 24 (USP24) protected p38-phosphorylated Runx2 (pRunx2) from ubiquitin-dependent proteasomal degradation, thereby enabling pRunx2 to recruit two transcriptional regulators, namely p300 (a histone acetyltransferase) and the nuclear receptor coactivator 3 (NCOA3), to assemble a NCOA3-p300-pRunx2 complex."
USP24 decreases the amount of RUNX2.
| 1
USP24 decreases the amount of RUNX2. 1 / 1
| 1

reach
"However, unlike HAUSP, USP24 was unable to interact with RUNX2 and reduce ubiquitination levels of RUNX2, suggesting that HAUSP, not USP24, functions as a bona-fide RUNX2-DUB."
USP24 affects EV71
| 8
USP24 activates EV71.
| 7
USP24 activates EV71. 7 / 7
| 7

reach
"Besides, USP24 promotes the immune evasion of EV71 by restricting the K63-linked polyubiquitination of TBK1(Zang et al., 2023)."

reach
"EV71 infection can lead to an increase in the expression of USP24, which in turn promotes EV71 infection."

reach
"Taken together, these findings revealed that EV71 infection can promote USP24 expression.3.2 USP24 promotes EV71 infection."

reach
"Furthermore, we found that knockdown of endogenous USP24 significantly suppressed the cytopathogenic effect of EV71 infection (Fig. 2G)."

reach
"Consequently, USP24 inhibits IFN-I production and promotes EV71 infection."

reach
"We next sought to determine the mechanism by which USP24 promotes EV71 infection."

reach
"As a consequence, USP24 knockdown dramatically inhibited EV71 infection."
USP24 inhibits EV71.
| 1
USP24 inhibits EV71. 1 / 1
| 1

reach
"As a consequence, USP24 knockdown improves IFN-I production and dramatically inhibits EV71 infection [162]."
USP24 affects BTRC
| 7 1
USP24 activates BTRC.
| 3
USP24 activates BTRC. 3 / 3
| 3

reach
"In cancer cells, USP24 increased p300 and beta-TrCP, thus increasing the acetylation of histone H3 and the degradation of DNMT1 and IkappaB, resulting in the recruitment of histone H3 acetylation to the promoter region of IL-6, the reduction of DNA methylation, and the promotion of NF-kappaB nuclear translocation, thereby facilitating IL-6 expression."

reach
"This study shows that USP24 mediates p300, NF-kappaB, DNMT1, and beta-TrCP to regulate IL-6 expression, thus affecting cancer metastasis."

reach
"To further clarify whether USP24 directly targets beta-TrCP for deubiquitination, an in vitro deubiquitination assay was performed, and purified human USP24 significantly decreased the beta-TrCP ubiquitination signal."
USP24 deubiquitinates BTRC.
| 2
USP24 deubiquitinates BTRC. 2 / 2
| 2

reach
"To further clarify whether USP24 directly targets beta-TrCP for deubiquitination, an in vitro deubiquitination assay was performed, and purified human USP24 significantly decreased the beta-TrCP ubiquitination signal."

reach
"These results provide evidence that USP24 directly deubiquitinates and stabilizes beta-TrCP."
USP24 binds BTRC.
| 1 1
| 1 1

reach
"Immunoprecipitation analysis confirmed the interaction between USP24 and beta-TrCP, and ubiquitinated beta-TrCP was significantly increased in USP24-knockdown A549 cells."

sparser
"Immunoprecipitation analysis confirmed the interaction between USP24 and β-TrCP (Fig.  xref ), and ubiquitinated β-TrCP was significantly increased in USP24-knockdown A549 cells (Fig.  xref and Supplementary Fig.  xref )."
USP24 increases the amount of BTRC.
| 1
USP24 increases the amount of BTRC. 1 / 1
| 1

reach
"Up-regulated USP24 increased the protein level of β-TrCP and p300 to promote lung cancer malignancy 31."
TBK1 affects USP24
1 | 7
1 | 7

No evidence text available

sparser
"Our above studies have demonstrated that USP24 interacts with TBK1."

sparser
"We found that USP24 could interact with TBK1, but not RIG-I, MAVS, TRAF6/3, or IRF3 ( xref A–F)."

sparser
"Furthermore, we confirmed that endogenous TBK1 could interact with endogenous USP24 in HEK293T cells ( xref G)."

sparser
"USP24 interacts with TBK1 to reduce the K63-linked polyubiquitination of TBK1, thereby affecting IRF3 activation and IFN-I production [ xref ]."

sparser
"Further studies revealed that USP24 physically interacted with TBK1, and can reduce K63-linked polyubiquitination of TBK1."

sparser
"Thus, this study revealed the regulation of the USP24-TBK1 axis on EV71 infection."

sparser
"USP24 can interact with TBK1."
EP300 affects USP24
1 | 3 4
EP300 binds USP24.
1 | 2 4
1 | 2 4

No evidence text available

reach
"Indeed, we observed that USP24 interacts with p300, and knockdown of USP24 increased p300 ubiquitination, and this effect of USP24 knockdown was abolished after treatment with the proteasome inhibitor MG132."

sparser
"The ubiquitin hydrolase USP24 directly interacts with p300 protein, and reduces p300 protein ubiquitination and proteasome-mediated degradation, leading to p300 protein up-regulation and histone acetylation xref (Figure xref C)."

sparser
"Our previous results also indicated that USP24 can interact with p300, an acetyltransferase that contains a BRD xref , xref ."

sparser
"Thus, GFP-USP24 increased p300 protein stability, whereas knockdown USP24 decreased its stability ( xref ; xref ) and USP24 interacted with p300 ( xref )."

sparser
"Indeed, we observed that USP24 interacts with p300 (Fig.  xref ), and knockdown of USP24 increased p300 ubiquitination, and this effect of USP24 knockdown was abolished after treatment with the proteasome inhibitor MG132 (Fig.  xref )."

reach
"Thus, GFP-USP24 increased p300 protein stability, whereas knockdown USP24 decreased its stability (XREF_FIG; XREF_SUPPLEMENTARY) and USP24 interacted with p300 (XREF_FIG)."
EP300 activates USP24.
| 1
EP300 activates USP24. 1 / 1
| 1

reach
"Overexpression of HA-p300 abolished the effect of USP24 knockdown on IL-6 expression."
USP24-i-101 affects USP24
| 7
USP24-i-101 inhibits USP24.
| 5
USP24-i-101 inhibits USP24. 5 / 5
| 5

reach
"One possibility is that USP24-i-101 treatment can rapidly inhibit all USP24 activity, but this activity is only partially silenced by shRNA, and this process requires 2-3 days."

reach
"USP24-i-101 blocks USP24 enzyme activity to decrease PD-1 protein stability."

reach
"In summary, an increase in the expression of USP24 in cancer cells is beneficial for the induction of drug resistance and targeting USP24 by USP24-i-101 optimized from USP24-i inhibits drug resistance acquired during cancer therapy by increasing PD-L1 protein degradation and genomic stability in an autophagy induction-dependent manner."

reach
"These findings suggest that USP24-i-101 may impair USP24 protein stability, implying that USP24 could control its own protein stability."

reach
"These data indicated that USP24-i-101 specifically blocks USP24 activity to decrease PD-1 stability.We then investigated whether USP24-i-101 increased T cell anticancer activity (fig."
USP24-i-101 decreases the amount of USP24.
| 1
USP24-i-101 decreases the amount of USP24. 1 / 1
| 1

reach
"However, USP24 expression is not down-regulated by USP24-i-101 in the presence of the proteasome inhibitor MG132 (Fig. 5B)."
USP24-i-101 activates USP24.
| 1
USP24-i-101 activates USP24. 1 / 1
| 1

reach
"Our previous reports revealed that the small-molecule inhibitor USP24-i-101 specifically inhibited USP24 enzyme activity to reverse chemotherapy resistance (20, 28)."
7 |
USP24 is inactive.
6 |
USP24 phosphorylated on S1616 is inactive. 2 / 2
2 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
USP24 phosphorylated on S2047 is inactive. 2 / 2
2 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
USP24 phosphorylated on S2604 is inactive. 2 / 2
2 |

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."

"Epidermal growth factor (EGF) treatment, and the KrasG12D and EGFRL858R mutations decrease USP24 protein stability via EGF- or CDK1-mediated phosphorylation at Ser1616, Ser2047 and Ser2604."
USP24 is active.
1 |
Phosphorylated USP24 is active. 1 / 1
1 |

"Taken together, these data suggest that the ATM kinase mediated phosphorylation of USP24 is involved in USP24 stabilization/up regulation following UV irradiation.|Taken together, these data suggest that the ATM kinase-mediated phosphorylation of USP24 is involved in USP24 stabilization/up-regulation following UV irradiation."
USP24 affects Infections
| 7
USP24 activates Infections.
| 5
| 5

reach
"Furthermore, we found that knockdown of endogenous USP24 significantly suppressed the cytopathogenic effect of EV71 infection (Fig. 2G)."

reach
"Consequently, USP24 inhibits IFN-I production and promotes EV71 infection."

reach
"We next sought to determine the mechanism by which USP24 promotes EV71 infection."

reach
"Taken together, these findings revealed that EV71 infection can promote USP24 expression.3.2 USP24 promotes EV71 infection."

reach
"EV71 infection can lead to an increase in the expression of USP24, which in turn promotes EV71 infection."
USP24 inhibits Infections.
| 2
| 2

reach
"As a consequence, USP24 knockdown dramatically inhibited EV71 infection."

reach
"As a consequence, USP24 knockdown improves IFN-I production and dramatically inhibits EV71 infection [162]."
USP24 affects BRD
| 7
| 7

sparser
"Because USP24 could interact with more than one BRD-containing protein, we hypothesized that USP24 can interact with the BRD, which is common to all BRD-containing proteins."

sparser
"In this study, we found that the deubiquitinase USP24 interacted with BRD through its C-terminus increased the levels of most BRD-containing proteins through increasing their protein stability by the removal of ubiquitin from Lys391/Lys400 of the BRD."

sparser
"Loss of the interaction motif of USP24 eliminated the ability of USP24 to stabilize BRD-containing proteins and abolished the effect of USP24 on cancer progression, including its inhibition of cancer cell proliferation and promotion of cancer cell migration, suggesting that the interaction between USP24 and the BRD is important for USP24-mediated effects on cancer progression."

sparser
"Finally, we also used a structural model of USP24 built based on USP7 as a parent template to study the structural interaction between USP24 and the BRD (Fig.  xref G)."

sparser
"Owing to the absence of structural information on the interaction between USP24 and the BRD, we generated a structural model of the catalytic domain of human USP24 (USP24-CD) by using the SWISS-MODEL server, and a complex structural model of the interaction between USP24-CD and the second BRD of human BRD2 (BRD2-BD2) was built up by using the ZDOCK server, which provided a structural view to understand the interactions between USP24-CD and BRD2-BD2 (Fig.  xref G(a))."

sparser
"BRD interacts with the C-terminus of USP24."

sparser
"2153) was sufficient for the interaction between USP24 and BRD (Fig.  xref B)."
IL6 affects USP24
| 6 1
IL6 increases the amount of USP24.
| 4 1
IL6 increases the amount of USP24. 5 / 5
| 4 1

reach
"Here, we demonstrate that environmental IL-6 promotes USP24 expression, suggesting a positive loop between IL-6 and USP24."

sparser
"Knockdown of RELA or STAT3 attenuated IL-6–induced USP24 mRNA expression ( xref and fig."

reach
"Increased interleukin-6 level transcriptionally activates the USP24 expression, which leads to PD-1 stabilization."

reach
"IL-6 increases USP24 expression to enhance PD-1 stability."

reach
"Knockdown of RELA or STAT3 attenuated IL-6–induced USP24 mRNA expression (Fig. 4J and fig."
IL6 activates USP24.
| 2
IL6 activates USP24. 2 / 2
| 2

reach
"IL-6 in the TME drives nuclear factor κB (NF-κB)/signal transducers and activators of transcription 3 (STAT3) signaling to transcriptionally increase USP24."

reach
"Deubiquitinase USP24 activated by IL-6/STAT3 enhances PD-1 protein stability and suppresses T cell antitumor response."
BRD affects USP24
| 7
| 7

sparser
"Because USP24 could interact with more than one BRD-containing protein, we hypothesized that USP24 can interact with the BRD, which is common to all BRD-containing proteins."

sparser
"In this study, we found that the deubiquitinase USP24 interacted with BRD through its C-terminus increased the levels of most BRD-containing proteins through increasing their protein stability by the removal of ubiquitin from Lys391/Lys400 of the BRD."

sparser
"Loss of the interaction motif of USP24 eliminated the ability of USP24 to stabilize BRD-containing proteins and abolished the effect of USP24 on cancer progression, including its inhibition of cancer cell proliferation and promotion of cancer cell migration, suggesting that the interaction between USP24 and the BRD is important for USP24-mediated effects on cancer progression."

sparser
"Finally, we also used a structural model of USP24 built based on USP7 as a parent template to study the structural interaction between USP24 and the BRD (Fig.  xref G)."

sparser
"Owing to the absence of structural information on the interaction between USP24 and the BRD, we generated a structural model of the catalytic domain of human USP24 (USP24-CD) by using the SWISS-MODEL server, and a complex structural model of the interaction between USP24-CD and the second BRD of human BRD2 (BRD2-BD2) was built up by using the ZDOCK server, which provided a structural view to understand the interactions between USP24-CD and BRD2-BD2 (Fig.  xref G(a))."

sparser
"BRD interacts with the C-terminus of USP24."

sparser
"2153) was sufficient for the interaction between USP24 and BRD (Fig.  xref B)."
USP24 affects TRAF6
| 4 2
USP24 binds TRAF6.
| 1 2
| 1 2

sparser
"The interaction between USP24 and TRAF6 in mitosis was studied (Fig.  xref )."

reach
"The interaction between USP24 and TRAF6 in mitosis was studied (Fig. 3F)."

sparser
"The data indicated that USP24 can interact with TRAF6 in mitotic A549 cells (Fig.  xref )."
USP24 inhibits TRAF6.
| 2
USP24 inhibits TRAF6. 2 / 2
| 2

reach
"In this study, we found that targeting USP24 by the specific USP24 inhibitors, USP24-i and its analogues, dramatically activated autophagy in the interphase and mitotic periods of lung cancer cells by inhibiting E2F4 and TRAF6, respectively."

reach
"In summary, downregulation of USP24 during the mitotic period may increase the degradation of TRAF6, thereby increasing the protein stability of beclin-1 and leading to autophagic induction (Fig. 3I)."
USP24 decreases the amount of TRAF6.
| 1
USP24 decreases the amount of TRAF6. 1 / 1
| 1

reach
"Targeting USP24 decreases E2F4 and TRAF6 levels to induce autophagy in interphase and mitotic cancer cells."
| 6
| 4

reach
"However, an interesting case-control study [86] investigating the synergistic effects of USP24, USP40, and UCH-L1 polymorphisms on PD risk in a Taiwanese population showed that a specific genotype of USP24 alone reduced PD susceptibility in subjects over the age of 60 years."

reach
"The USP24 T allele also seemed to reduce PD risk independently in the group> = 60 years (OR = 0.64, 95% CI 0.44-0.91, P = 0.023)."

reach
"Our results demonstrated that USP24 rs487230 T allele and CT/TT genotype notably decreased the risk of developing PD in the AAO> = 60 year subgroup of patients."

reach
"Interestingly, it has been reported that some polymorphisms of USP24 and USP40 significantly decrease the risk of developing PD [22,23]."
USP24 ubiquitinates Parkinson Disease.
| 1
USP24 leads to the ubiquitination of Parkinson Disease. 1 / 1
| 1

reach
"Variants in USP24 and USP40 are implicated in the risk for late-onset Parkinson’s disease (PD), supporting the proposed role of ubiquitination pathways in PD pathogenesis (25)."

reach
"However, the association between USP24 and PD is still controversial based on several association studies, which necessitates further investigation of this DUB."
USP24 affects PLK1
| 4 2
USP24 activates PLK1.
| 3
USP24 activates PLK1. 3 / 3
| 3

reach
"Ubiquitin-specific peptidase 24 accelerates aerobic glycolysis and tumor progression in gastric carcinoma through stabilizing PLK1 to activate NOTCH1."

reach
"36 Besides, Zhi et al. revealed that the knockdown of ubiquitin‐specific protease 24 (USP24) reduced the stability of PLK1 and consequently decreased the activity of NOTCH1, resulting in alleviating tumor progression."

reach
"A recent publication found that USP24 accelerates aerobic glycolysis and tumor progression in gastric carcinoma through stabilizing PLK1 to activate NOTCH1 [28]."
USP24 binds PLK1.
| 2
| 2

sparser
"HEK293T cells were co‐transfected with HA‐USP24 and Flag‐PLK1, and immunoprecipitation assay showed that USP24 and PLK1 interacted (Figure  xref )."

sparser
"USP24 interacts with PLK1."
USP24 increases the amount of PLK1.
| 1
USP24 increases the amount of PLK1. 1 / 1
| 1

reach
"Further analysis and study showed that USP24 could promote the stability and increase the expression of oncogene PLK1."
USP24 affects DNMT1
| 6
USP24 inhibits DNMT1.
| 3
USP24 inhibits DNMT1. 3 / 3
| 3

reach
"USP24 decreases DNMT1 and IkappaB through stabilizing beta-TrCP."

reach
"Finally, we found that knockdown of DNMT1 in USP24-knockdown A549 cells can rescue IL-6 level, suggesting that USP24 mediated DNMT1 degradation is really involved in IL-6 expression."

reach
"In this study, we observed that USP24 increased DNMT1 degradation in cancer cells, but not in M2 macrophages, leading to decreased DNA methylation."
USP24 decreases the amount of DNMT1.
| 2
USP24 decreases the amount of DNMT1. 2 / 2
| 2

reach
"beta-TrCP overexpression in USP24-knockdown cells prevented the upregulation of DNMT1and IkappaB induced by USP24 knockdown and demonstrated that beta-TrCP is critical in the USP24 induced increase of DNMT1 and IkappaB expression."

reach
"Furthermore, USP24 stabilizes p300 and reduces the levels of DNMT1, thereby increasing IL-6 transcription in M2 macrophages and lung cancer cells (107)."
USP24 activates DNMT1.
| 1
USP24 activates DNMT1. 1 / 1
| 1

reach
"This study shows that USP24 mediates p300, NF-kappaB, DNMT1, and beta-TrCP to regulate IL-6 expression, thus affecting cancer metastasis."
USP24 affects CD274
| 5 1
USP24 increases the amount of CD274.
| 2
USP24 increases the amount of CD274. 2 / 2
| 2

reach
"Knockdown of USP24 in PC9 and PC9-GR cells significantly decreased the level of PD-L1 (Fig. 6D)."

reach
"Genomic instability and PD-L1 levels were increased in drug resistant lung cancer cells and were inhibited by USP24-i-101 treatment or knockdown of USP24."
USP24 decreases the amount of CD274.
| 2
USP24 decreases the amount of CD274. 2 / 2
| 2

reach
"Targeting USP24 by USP24-i in PC9-GR cells decreased the level of PD-L1 (Fig. 6C)."

reach
"Targeting USP24 by USP24-i-101 induces autophagy in interphase and mitosis by inhibiting E2F4 and TRAF6, respectively, thereby inhibiting the expression of ABCG2 and PD-L1 and maintaining genomic integrity, resulting in the inhibition of drug resistance acquired by gefitinib- or Taxol-treatment of lung cancer (Fig. 8D)."
USP24 binds CD274.
| 1
| 1

sparser
"Interestingly, we found that USP24 can interact with PD-L1 in interphase and mitotic cells, implying that USP24 may stabilize PD-L1 in cancer drug-resistant cells PC9-GR (Fig.  xref ) and A549-T24 cells (Fig.  xref )."
USP24 activates CD274.
| 1
USP24 activates CD274. 1 / 1
| 1

reach
"These results prompted us to examine whether USP24 inhibition blocks PD-1/PD-L1–mediated T cell inhibition."
TP53 affects USP24
1 | 4 1
TP53 binds USP24.
1 | 2 1
1 | 2 1

sparser
"Third, co-immunoprecipitation assays showed that USP24 interacts with p53."

reach
"This third, independent assay format thus supports a direct interaction between USP24, p53 and orf10."

reach
"Third, co-immunoprecipitation assays showed that USP24 interacts with p53."

No evidence text available
TP53 inhibits USP24.
| 1
TP53 inhibits USP24. 1 / 1
| 1

reach
"These results indicate that USP24 promotes UV induced apoptosis in HCT116 cells, consistent with the notion that p53 directed apoptosis is attenuated in USP24 depleted cells which lack robust p53 stabilization after UV treatment."
TP53 activates USP24.
| 1
TP53 activates USP24. 1 / 1
| 1

reach
"It has been shown that USP24 targets p53 to regulate UV induced apoptosis."
RUNX2 affects USP24
3 | 1 2
3 | 1 2

No evidence text available

No evidence text available

No evidence text available

sparser
"Mutation of the serine-28 site of Runx2 to aspartate (D) to mimic Runx2 phosphorylation revealed that USP24 directly interacted with Runx2 S28D rather than Runx2 in NP-1 cells co-expressing Flag Runx2 S28D + MYC USP24 (Fig.  xref E)."

sparser
"Nevertheless, screening inhibitors that target p300-Runx2 S28D and Runx2 S28D -USP24 interactions appears to be a promising strategy for specifically decreasing ADAMTS expression."

reach
"To examine endogenous interaction between RUNX2 and CK2 or USP24, cell lysates were obtained from mouse COBs and prepared in lysis buffer (50mM Tris-HCl (pH 7.4), 150mM NaCl, 1% Triton X-100, 1mM EDTA, 1mM EGTA, 50mM NaF, 1mM Na 3 VO 4, 1mM PMSF, and protease inhibitor cocktail (Sigma))."
ATM affects USP24
2 | 2 1
ATM activates USP24.
1 | 2
ATM activates USP24. 3 / 4
1 | 2

reach
"Taken together, these data suggest that the ATM kinase mediated phosphorylation of USP24 is involved in USP24 stabilization/up regulation following UV irradiation."

"Taken together, these data suggest that the ATM kinase mediated phosphorylation of USP24 is involved in USP24 stabilization/up regulation following UV irradiation.|Taken together, these data suggest that the ATM kinase-mediated phosphorylation of USP24 is involved in USP24 stabilization/up-regulation following UV irradiation."

reach
"Moreover, UV induced USP24 accumulation appears to be ATM dependent; inhibition of ATM by either KU-55933 or a specific siRNA prevented USP24 accumulation after UV (XREF_FIG)."
ATM phosphorylates USP24.
1 | 1
ATM phosphorylates USP24. 2 / 2
1 | 1

"Taken together, these data suggest that the ATM kinase mediated phosphorylation of USP24 is involved in USP24 stabilization/up regulation following UV irradiation.|Taken together, these data suggest that the ATM kinase-mediated phosphorylation of USP24 is involved in USP24 stabilization/up-regulation following UV irradiation."

sparser
"Taken together, these data suggest that the ATM kinase-mediated phosphorylation of USP24 is involved in USP24 stabilization/up-regulation following UV irradiation."
USP24 affects cell death
| 5
USP24 activates cell death.
| 4
| 4

reach
"Direct Usp24 KD resulted in marked induction of myeloma cell death that was associated with a reduction of Mcl-1."

reach
"Previous studies have demonstrated that USP24 inhibited proliferation and migration of HCC, and our findings also indicated that USP24 increased cell death in HCC cells (Supplementary Fig. 4A)."

reach
"GFP-USP24 expression, but not GFP, led to rounded cells and, finally, cell death."

reach
"Based on these results, targeting USP24-induced cell death occurs in an autophagy-dependent but apoptosis-independent manner."
USP24 inhibits cell death.
| 1
| 1

reach
"Knockdown of USP24 suppressed the cell death during pUL38 deficient HCMV infection, suggesting that pUL38 achieved its function by antagonizing the function of USP24."
USP24 affects UBC
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP24 affects TRAF2
| 5
USP24 deubiquitinates TRAF2.
| 3
USP24 deubiquitinates TRAF2. 3 / 3
| 3

reach
"Another study clarified that USP24 facilitates the oncogenesis of HCC by deubiquitinating and stabilizing TRAF2 [30]."

reach
"During our research process, another study clarified that USP24 facilitates the oncogenesis of HCC by deubiquitinating and stabilizing TRAF2 [30]."

reach
"In hepatocellular carcinoma (HCC), the ubiquitin-specific peptidase 24 (USP24) deubiquitinates and stabilizes TRAF2, which, in turn, promotes cell proliferation and metastasis [107, 108]."
USP24 inhibits TRAF2.
| 2
USP24 bound to tumor necrosis factor receptor inhibits TRAF2. 1 / 1
| 1

reach
"Mechanistically, USP24 binds to tumor necrosis factor receptor-associated factor 2(TRAF2) and inhibits its degradation, thereby promoting the accumulation of TRAF2."
USP24 bound to TNF inhibits TRAF2. 1 / 1
| 1

reach
"Mechanistically, USP24 binds to tumor necrosis factor receptor-associated factor 2(TRAF2) and inhibits its degradation, thereby promoting the accumulation of TRAF2."
USP24 affects STAT3
| 5
USP24 activates STAT3.
| 3
USP24 bound to GSDMB activates STAT3. 2 / 2
| 2

reach
"Here, our results indicated that USP24 bound to GSDMB to stabilize GSDMB, and subsequently activated the STAT3 pathway in bladder cancer cells."

reach
"In bladder cancer, the USP24/GSDMB/STAT3 axis is reported to promote tumor proliferation and growth, where USP24 interacts and stabilizes GSDMB, which in turn binds to STAT3, increases its phosphorylation and activates STAT3 signaling (30)."
USP24 activates STAT3. 1 / 1
| 1

reach
"Besides, we also showed that USP24 stabilized GSDMB to activate STAT3 signaling, which was blocked by the USP24 inhibitor.Conclusions: We suggested that aberrantly up-regulated GSDMB was responsible for enhancing the growth and invasion ability of bladder cancer cells."
USP24 phosphorylates STAT3.
| 2
USP24 leads to the phosphorylation of STAT3. 2 / 2
| 2

reach
"In conclusion, USP24 stabilizes GSDMB to promote STAT3 phosphorylation in bladder cancer cells (Fig. 6L)."

reach
"USP24 stabilizes GSDMB to promote STAT3 phosphorylation in bladder cancer cells."
USP24 affects BRD7
1 | 4
1 | 3

sparser
"Therefore, we first constructed an N-terminal USP24 construct, USP24-(1-2–3) and a C-terminal USP24 construct, USP24-(4), to study the interaction of USP24 with BRD7 (Fig.  xref A,C)."

sparser
"First, we found that endogenous USP24 could interact with BRD7 in H1299 cells and mouse embryonic fibroblasts (MEFs) (Fig.  xref A and Suppl."

No evidence text available

sparser
"The data indicated that BRD7 interacted with full-length USP24 and the C-terminus of USP24, USP24-(4), but not USP24-(1-2-3), which is consistent with the results of the yeast two-hybrid assay."
| 1

sparser
"Next, when HA-BRD7 and HA-BRG1 were expressed in H1299 cells or MEFs, we found that USP24 interacted with BRD7 and BRG1, indicating that USP24 could interact with more than one BRD-containing protein in different cell lines (Fig.  xref B,C and Suppl."
UBC affects USP24
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
EOAI3402143 affects USP24
| 1 4
EOAI3402143 inhibits USP24. 5 / 5
| 1 4

reach
"A more novel inhibitor EOAI3402143 inhibited Usp9x and Usp24 activity in a dose dependent manner and resulted in the induction of apoptosis."

eidos
"Another novel small molecule , EOAI3402143 ( with properties similar to WP1130 ) , selectively inhibits USP9X and USP24 , induces apoptosis in malignant B-cell lines , and also blocks or regresses myeloma tumors in mice [ 127 ] ."

reach
"Furthermore, we improved the drug like properties of WP1130 and demonstrated that the novel compound EOAI3402143 dose-dependently inhibited Usp9x and Usp24 activity, increased tumor cell apoptosis, and fully blocked or regressed myeloma tumors in mice."

reach
"The novel inhibitor EOAI3402143 proved to inhibit both Usp9x and Usp24 activity and suppresses tumor growth in vivo [XREF_BIBR]."

reach
"Peterson et al (118) reported on a small molecule inhibitor of USP9X/USP24, EOAI3402143(G9), which inhibited the DUB activity of USP9X and USP24 in a dose-dependent manner, increased apoptosis in myeloma and fully regressed or blocked myeloma tumors in mice."
E2F4 affects USP24
| 3 2
E2F4 binds USP24.
| 2 2
| 2 2

reach
"Further study indicated that USP24 could interact with E2F4 (XREF_FIG)."

reach
"Further studies have demonstrated the interaction between USP24 and E2F4."

sparser
"Further studies have demonstrated the interaction between USP24 and E2F4."

sparser
"Further study indicated that USP24 could interact with E2F4 ( xref )."
E2F4 activates USP24.
| 1
E2F4 activates USP24. 1 / 1
| 1

reach
"Overexpression of E2F4 increased the mRNA level of E2F1 in USP24-silenced cells, partially inhibiting the G1-S transition and cell proliferation."
BRD7 affects USP24
1 | 4
1 | 3

sparser
"Therefore, we first constructed an N-terminal USP24 construct, USP24-(1-2–3) and a C-terminal USP24 construct, USP24-(4), to study the interaction of USP24 with BRD7 (Fig.  xref A,C)."

sparser
"First, we found that endogenous USP24 could interact with BRD7 in H1299 cells and mouse embryonic fibroblasts (MEFs) (Fig.  xref A and Suppl."

No evidence text available

sparser
"The data indicated that BRD7 interacted with full-length USP24 and the C-terminus of USP24, USP24-(4), but not USP24-(1-2-3), which is consistent with the results of the yeast two-hybrid assay."
| 1

sparser
"Next, when HA-BRD7 and HA-BRG1 were expressed in H1299 cells or MEFs, we found that USP24 interacted with BRD7 and BRG1, indicating that USP24 could interact with more than one BRD-containing protein in different cell lines (Fig.  xref B,C and Suppl."
USP9X affects USP24
| 3 1
USP9X inhibits USP24.
| 3
USP9X inhibits USP24. 3 / 3
| 3

reach
"Usp9x depletion induced upregulation of Usp24 which has shown to be involved in myeloma cell survival."

reach
"Moreover, the decreasing of USP24 could be rescued by MG132, indicating that USP9X can promote the degradation of USP24."

reach
"In order to determine whether USP9X promote the degradation of USP24, we over-expressed USP9X in 293T cells and examined the protein level of USP24."
USP9X binds USP24.
| 1
| 1

sparser
"Indeed, our pull-down studies for NOTCH1, followed by detection of USP24 and USP9x in western blot experiments, showed that neither USP9x nor USP24 interact with NOTCH1 ( xref )."

reach
"In conclusion, USP24 was upregulated in GC and promoted the growth and glycolysis of GC cells, the mechanism of which was related to the deubiquitination of PLK1 and the increase of its stability."

reach
"A recent publication found that USP24 accelerates aerobic glycolysis and tumor progression in gastric carcinoma through stabilizing PLK1 to activate NOTCH1 [28]."

reach
"Ubiquitin-specific peptidase 24 accelerates aerobic glycolysis and tumor progression in gastric carcinoma through stabilizing PLK1 to activate NOTCH1."

reach
"It was found that overexpressed USP24 promoted GC cell proliferation and abnormal glycolysis."
USP24 affects WP1130
| 4
WP1130 binds USP24. 4 / 4
| 4

reach
"In order to investigate whether the WP1130 directly interacts with USP24, a cellular thermal shift assay (CETSA) was conducted [XREF_BIBR, XREF_BIBR]."

reach
"These data suggest that WP1130 directly interacts with USP24 in T-ALL cells."

reach
"WP1130 interacts with USP24 in cells."

reach
"To elucidate the interaction between WP1130 and USP24, a molecular docking study was performed."
USP24 affects TFDP1
| 4
USP24 inhibits TFDP1.
| 1
USP24 inhibits TFDP1. 1 / 1
| 1

reach
"Further experiments reveal that USP24 knockdown decreases the stability of E2F4 protein, p130, and TFDP1, resulting in an increase in E2F1 levels and a promotion of the G1-S transition, ultimately lea[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 increases the amount of TFDP1.
| 1
Modified USP24 increases the amount of TFDP1. 1 / 1
| 1

reach
"The molecular mechanism involves a decrease in the USP24 level, which reduces the expression of E2F4 and its partner TFDP1, and thus increases the G1/S transition."
USP24 decreases the amount of TFDP1.
| 1
USP24 decreases the amount of TFDP1. 1 / 1
| 1

reach
"Knockdown of USP24 decreased the E2F4, p130 and TFDP1 levels, which form a complex to regulate the G1-S transition, but did not alter the E2F4 and TDFP1 mRNA levels (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 activates TFDP1.
| 1
USP24 activates TFDP1. 1 / 1
| 1

reach
"Taken together, our results demonstrated that USP24 knockdown decreased E2F4, p130 and TFDP1, resulting in an increase in the E2F1 levels and a subsequent increase in the G1-S transition, leading to tumorigenesis."
USP24 affects SUV39H1
2 | 2
USP24 binds SUV39H1.
2 |
2 |

No evidence text available

No evidence text available
USP24 increases the amount of SUV39H1.
| 1
USP24 increases the amount of SUV39H1. 1 / 1
| 1

reach
"USP24 was also found to target histones by controlling the levels of the histone-lysine N-methyltransferase Suv39h1, resulting in a modulation of the H3K9me levels."
USP24 decreases the amount of SUV39H1.
| 1
USP24 decreases the amount of SUV39H1. 1 / 1
| 1

reach
"Moreover, knockdown of USP24 increases Suv39h1 expression, which results in enhanced H3K9me levels and prevents the progression of malignant lung cancer (63)."
USP24 affects NFkappaB
| 3 1
USP24 activates NFkappaB.
| 2 1
| 2 1

reach
"In this study, we discovered the underlying mechanisms by which USP24 not only promotes IkappaB degradation by stabilizing beta-transduction repeat containing E3 ubiquitin protein ligase (beta-TrCP) in lung cancer cells but also induces the upregulation of NF-kappaB in M2 macrophages, resulting in an increase in IL-6 expression."

reach
"This study shows that USP24 mediates p300, NF-kappaB, DNMT1, and beta-TrCP to regulate IL-6 expression, thus affecting cancer metastasis."

sparser
"However, in this study, USP24-activated NF-κB did not increase CCL2, implying that other factors regulated by USP24 might negatively regulate CCL2 expression."
USP24 increases the amount of NFkappaB.
| 1
USP24 increases the amount of NFkappaB. 1 / 1
| 1

reach
"In M2 macrophages, USP24 increased p300 levels, subsequently enhancing the levels of NF-kappaB and IL-6."
USP24 affects IKB
| 4
USP24 inhibits IKB.
| 3
USP24 inhibits IKB. 3 / 3
| 3

reach
"In this study, we discovered the underlying mechanisms by which USP24 not only promotes IkappaB degradation by stabilizing beta-transduction repeat containing E3 ubiquitin protein ligase (beta-TrCP) in lung cancer cells but also induces the upregulation of NF-kappaB in M2 macrophages, resulting in an increase in IL-6 expression."

reach
"beta-TrCP overexpression in USP24-knockdown cells prevented the upregulation of DNMT1and IkappaB induced by USP24 knockdown and demonstrated that beta-TrCP is critical in the USP24 induced increase of DNMT1 and IkappaB expression."

reach
"USP24 decreases DNMT1 and IkappaB through stabilizing beta-TrCP."
USP24 increases the amount of IKB.
| 1
USP24 increases the amount of IKB. 1 / 1
| 1

reach
"beta-TrCP overexpression in USP24-knockdown cells prevented the upregulation of DNMT1and IkappaB induced by USP24 knockdown and demonstrated that beta-TrCP is critical in the USP24 induced increase of DNMT1 and IkappaB expression."
USP24 affects Histone_H3
| 4
USP24 acetylates Histone_H3.
| 3
USP24 leads to the acetylation of Histone_H3. 3 / 3
| 3

reach
"As an example, USP24, a deubiquitinating enzyme upregulated in M2 macrophages of lung cancer cells, could stabilize HAT p300 thus increase H3 acetylation and NF-κB while decrease DNMT1, subsequently elevating TNF-α and IL-6 transcription, ultimately resulting in cancer malignancy [175]."

reach
"It has been described that the cysteine protease USP24 stabilizes p300 and thereby increases acetylation of histone h3 (Wang et al. 2018)."

reach
"In cancer malignancy, USP24 stabilizes p300, increases the levels of H3 acetylation and NF-κB, and induces IL-6 (51)."
USP24 increases the amount of Histone_H3.
| 1
USP24 increases the amount of Histone_H3. 1 / 1
| 1

reach
"In cancer malignancy, USP24 stabilizes p300, increases the levels of H3 acetylation and NF-κB, and induces IL-6 (51)."
| 3

reach
"To investigate whether USP24 knockdown suppressed the tumorigenesis of HCC cells in vivo, we injected Bel-7402 cells which were stably knockdown USP24 (shUSP24-2) or the negative control (shNC) into the subcutaneous of the nude mice."

reach
"USP24 promotes hepatocellular carcinoma tumorigenesis through deubiquitinating and stabilizing TRAF2."

reach
"These results indicated that functional depletion of USP24 attenuates tumorigenesis in TetO-Egfr mice, supporting its oncogenic role.Next, we explored whether USP24 inactivation promotes antitumor immunity."
USP24 affects CDC20
| 2 2
| 2 2

sparser
"Moreover, cdc20 could also interact with USP24 when these residues were phosphorylated, implying that the decrease in USP24 during mitosis might be important for cell cycle progression and cancer cell proliferation ( xref )."

sparser
"Further study found that USP24 interacted with cdc20 during the mitotic stage ( xref ; xref )."

reach
"Further study found that USP24 interacted with cdc20 during the mitotic stage (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"Moreover, cdc20 could also interact with USP24 when these residues were phosphorylated, implying that the decrease in USP24 during mitosis might be important for cell cycle progression and cancer cell proliferation (XREF_FIG)."
SIRT7 affects USP24
2 | 2
2 | 2

No evidence text available

No evidence text available

sparser
"miR-21-5p promoted the expression of ubiquitination-specific peptidase 24 (USP24); the interaction between USP24 and SIRT7 decreased the level of SIRT7 ubiquitination in HCC cells, while upregulating the LC3-II/I ratio and beclin-1 level."

sparser
"Our results demonstrated that USP24 interacts with SIRT7, and that overexpression of USP24 significantly reduced SIRT7 polyubiquitination."
PDCD1 affects USP24
| 1 3
| 1 3

sparser
"We hypothesized that USP24 directly interacts with PD-1 to cleave ubiquitin on PD-1."

sparser
"IP-Western results further confirmed that IL-6 treatment increased interaction between USP24 and PD-1 (fig."

sparser
"S3C), indicating that USP24 binds to the intracellular region of PD-1 to facilitate deubiquitination."

reach
"IP-Western results further confirmed that IL-6 treatment increased interaction between USP24 and PD-1 (fig."
MCL1 affects USP24
| 1 3
| 1 3

reach
"It has been observed that both USP9X and USP24 can bind to MCL-1 and enhance its stability."

sparser
"The mechanism is that USP24-MCL-1 axis accelerates the breakdown of mitochondrial transmembrane potential and induces apoptosis."

sparser
"Our laboratory’s previous study reported that USP24 plays an important role in the survival of T-cell acute lymphoblastic leukemia (T-ALL) cells and targeting USP24-Mcl-1 axis may represent a novel strategy in the treatment of T-ALL [ xref ]."

sparser
"In T-ALL, USP24 exhibits a negative association with the prognosis of T-ALL and stabilizes Mcl-1 protein by deubiquitylating MCL-1 via the USP24-MCL-1 axis."
Infections affects USP24
| 4
Infections increases the amount of USP24.
| 2
Infections increases the amount of USP24. 2 / 2
| 2

reach
"3.1 EV71 infection significantly promotes the expression of the deubiquitinase USP24."

reach
"In this paper, we reveal that EV71 infection promotes host USP24 gene expression and therefore inhibits IFN-I antiviral immune response."
Infections activates USP24.
| 2
| 2

reach
"Taken together, these findings revealed that EV71 infection can promote USP24 expression.3.2 USP24 promotes EV71 infection."

reach
"Importantly, USP24 was upregulated by EV71 infection, which suggested a novel regulatory mechanism of the RIG-I/MAVS signaling pathway."
EV71 affects USP24
| 4
EV71 increases the amount of USP24.
| 2
EV71 increases the amount of USP24. 2 / 2
| 2

reach
"In this paper, we reveal that EV71 infection promotes host USP24 gene expression and therefore inhibits IFN-I antiviral immune response."

reach
"In this study, we found that EV71 upregulated the expression of USP24 in human rhabdomyosarcoma cells (RD)."
EV71 activates USP24.
| 2
EV71 activates USP24. 2 / 2
| 2

reach
"However, it is possible that other proteins of EV71 upregulate USP24 expression.5 Conclusions."

reach
"Taken together, these findings revealed that EV71 infection can promote USP24 expression.3.2 USP24 promotes EV71 infection."
CDC20 affects USP24
| 2 2
| 2 2

sparser
"Moreover, cdc20 could also interact with USP24 when these residues were phosphorylated, implying that the decrease in USP24 during mitosis might be important for cell cycle progression and cancer cell proliferation ( xref )."

sparser
"Further study found that USP24 interacted with cdc20 during the mitotic stage ( xref ; xref )."

reach
"Further study found that USP24 interacted with cdc20 during the mitotic stage (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"Moreover, cdc20 could also interact with USP24 when these residues were phosphorylated, implying that the decrease in USP24 during mitosis might be important for cell cycle progression and cancer cell proliferation (XREF_FIG)."
3 |
Sodium arsenite decreases the amount of USP24.
2 |
Sodium arsenite decreases the amount of USP24. 2 / 2
2 |

No evidence text available

No evidence text available
Sodium arsenite increases the amount of USP24.
1 |
Sodium arsenite increases the amount of USP24. 1 / 1
1 |

No evidence text available
Orf10 affects USP24
| 3
Orf10 binds USP24. 3 / 3
| 3

sparser
"KSHV orf10 interacted with the deubiquitinase USP24 and with p53 itself, and p53 is a known substrate for USP24 [ xref ]."

sparser
"These experiments confirm the prior reports of the KSHV orf10-USP24 interaction in a second, independent cell line."

sparser
"To test this hypothesis, the published orf10 and USP24 interaction was verified in p53 wild-type U2OS cells."
MiR-139-5p affects USP24
| 3
MiR-139-5p decreases the amount of USP24.
| 2
MiR-139-5p decreases the amount of USP24. 2 / 2
| 2

reach
"Further, miR-139-5p inhibited the expression of the deubiquitinating enzyme USP24 (41), which negatively regulates type I IFN response and autoimmune response (76)."

reach
"Furthermore, miR-139-5p suppressed the expression of the deubiquitinating enzyme USP24, which is a cofactor for TLR7/9 signaling [69,106]."
MiR-139-5p inhibits USP24.
| 1
MiR-139-5p inhibits USP24. 1 / 1
| 1

reach
"It was found that miR-139-5p could inhibit USP24 not only in vivo but also in vitro."
XRCC6 affects USP24
| 3
| 3

reach
"The data revealed that USP24 interacted with Ku70 (XREF_FIG)."

reach
"15 Herein, we used a yeast two-hybrid assay to study the interaction between Ku70 and USP24 (XREF_FIG; XREF_SUPPLEMENTARY)."

reach
"XREF_BIBR, XREF_BIBR Our preliminary results from a yeast two-hybrid assay showed that Ku70 interacts with USP24; however, the role of USP24 in Bax mediated apoptosis is unknown."
USP24 affects orf10
| 3
Orf10 binds USP24. 3 / 3
| 3

sparser
"KSHV orf10 interacted with the deubiquitinase USP24 and with p53 itself, and p53 is a known substrate for USP24 [ xref ]."

sparser
"These experiments confirm the prior reports of the KSHV orf10-USP24 interaction in a second, independent cell line."

sparser
"To test this hypothesis, the published orf10 and USP24 interaction was verified in p53 wild-type U2OS cells."
USP24 affects miR-21-5p inhibitor
| 3
USP24 inhibits miR-21-5p inhibitor. 3 / 3
| 3

reach
"The results of the EdU and Transwell assays showed that the addition of the miR-21-5p inhibitor significantly inhibited cell proliferation and migration, that USP24 overexpression could reverse the ef[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The Western blot results showed that the miR-21-5p inhibitor markedly restrained the levels of LC3II/I ratio and Beclin1 and elevated the level of p62, and overexpression of USP24 rescued the influenc[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further immunofluorescence detection showed that the addition of the miR-21-5p inhibitor significantly inhibited autophagy, that overexpression of USP24 rescued the influence of the miR-21-5p inhibito[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 affects USP24
| 2
USP24 increases the amount of USP24.
| 1
USP24 increases the amount of USP24. 1 / 2
| 1

reach
"XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR Our recent study showed that USP24 variants from SNPs and RNA editing products increased the levels of USP24 and MDM2, which regulates Suv39h1 in lung cancer cells, subsequently resulting in an increase in metastatic activities during lung cancer progression."
USP24 inhibits USP24.
| 1
USP24 inhibits USP24. 1 / 1
| 1

reach
"Here we found that the phosphorylation of several USP24 residues was regulated by EGF treatment and mitotic CDK1 to enhance USP24 degradation in a polyubiquitination dependent manner."
| 3

reach
"It was found that overexpressed USP24 promoted GC cell proliferation and abnormal glycolysis."

reach
"In conclusion, USP24 was upregulated in GC and promoted the growth and glycolysis of GC cells, the mechanism of which was related to the deubiquitination of PLK1 and the increase of its stability."

reach
"Silencing USP24 inhibited the GC process."
USP24 affects SQSTM1
| 3
USP24 activates SQSTM1.
| 2
USP24 activates SQSTM1. 2 / 2
| 2

reach
"Knockdown of USP24 increased the rate of degradation of the autophagy adaptor protein SQSTM1 and p62)."

reach
"Knockdown of USP24 increased the rate of degradation of the autophagy adaptor protein SQSTM1 and p62)."
USP24 decreases the amount of SQSTM1.
| 1
USP24 decreases the amount of SQSTM1. 1 / 1
| 1

reach
"As illustrated in Fig. 3D, E, knocking down USP24 inhibited the expression of ULK1, Atg5, and Beclin1 proteins, whereas overexpression of USP24 increased the protein expression of autophagic molecules as well as LC3B-II, but decreased the expression of p62."
USP24 affects RP2
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
USP24 affects RHOJ
3 |
3 |

No evidence text available

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No evidence text available
USP24 affects PARP1
| 3
USP24 inhibits PARP1.
| 2
USP24 inhibits PARP1. 2 / 2
| 2

reach
"In p53 +/+ cells, cleaved PARP started to appear as early as three hours after UV irradiation and depletion of USP24 resulted in slow and reduced PARP cleavage (XREF_FIG, right panel)."

reach
"USP24 depletion inhibits PUMA activation and PARP cleavage in response to UV damage."
USP24 activates PARP1.
| 1
USP24 activates PARP1. 1 / 1
| 1

reach
"To examine whether USP24 regulates the apoptotic response to UV through the p53 pathway, we compared the effects of USP24 depletion on UV induced PARP cleavage in two isogenic cell lines : HCT116 p53 +/+ and HCT116 p53-/-."

reach
"After IL-6 was added to the M2 macrophages conditioned medium, this effect was rescued, indicating that the USP24 induced metastasis activity is due to upregulated IL-6, which can be expressed by M2 macrophages or cancer cells."

reach
"We previously revealed that USP24 promotes lung tumor metastasis and enhances IL-6 secretion in TAMs to contribute to an immunosuppressive TME (18, 19)."

reach
"These findings further implied that NF-kappaB is indeed involved in USP24 promoted metastasis."
USP24 affects NCOA4
| 3
USP24 inhibits NCOA4.
| 1
USP24 inhibits NCOA4. 1 / 1
| 1

reach
"Knockdown of USP24 decreased NCOA4 protein stability and ferritin heavy chain degradation in lysosomes."
USP24 increases the amount of NCOA4.
| 1
USP24 increases the amount of NCOA4. 1 / 1
| 1

reach
"Interestingly, studies have shown that USP24 and PUL38 collectively increase the level of NCOA4 and involved in the regulation of ferritinophagy during HCMV infection."
USP24 activates NCOA4.
| 1
USP24 activates NCOA4. 1 / 1
| 1

reach
"USP24 knockdown decreased NCOA4 stability and FTH1 degradation."
USP24 affects N
3 |
N binds USP24. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
| DOI
USP24 affects LYPD3
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
USP24 affects Interferon
| 3
| 3

reach
"Moreover, miR-139-5p affects USP24 that negatively regulates type I IFN response [ 239 ]."

reach
"Further, miR-139-5p inhibited the expression of the deubiquitinating enzyme USP24 (41), which negatively regulates type I IFN response and autoimmune response (76)."

reach
"We found that USP24 knockdown promoted the mRNA expression of IFNβ induced by EV71 infection (Fig. 3A)."
USP24 affects IRF3
| 3
USP24 decreases the amount of IRF3.
| 2
USP24 decreases the amount of IRF3. 2 / 2
| 2

reach
"We found that knockdown of USP24 upregulated the phosphorylation levels of IRF3 induced by EV71 infection, but did not affect the protein levels of IRF3 (Fig. 4A)."

reach
"The results showed that USP24 knockdown upregulated nuclear IRF3 levels during EV71 infection (Fig. 4B)."
USP24 deubiquitinates IRF3.
| 1
USP24 leads to the deubiquitination of IRF3. 1 / 1
| 1

reach
"Here, we found that USP24 can downregulate TBK1 ubiquitination and influence IRF3 activation during EV71 infection."
USP24 affects IFN-I
| 3
USP24 inhibits IFN-I. 3 / 3
| 3

reach
"Consequently, USP24 inhibits IFN-I production and promotes EV71 infection."

reach
"Furthermore, we revealed the mechanism by which USP24 inhibits the production of IFN-I to achieve immune escape during EV71 infection.2 Materials & methods."

reach
"In this paper, we reveal that EV71 infection promotes host USP24 gene expression and therefore inhibits IFN-I antiviral immune response."
USP24 affects HIF1AN
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

reach
"Increased levels of USP24 lead to p53 stabilization and upregulation and subsequent activation of p53 downstream targets.can be reversed by the action of DUBs.Thus, targeting DUBs may have promising potential in cancer therapy.9 USP24offers novel exciting perspectives in basicand translational research, and anapoptosis and genome stability."

reach
"LC3-II was significantly increased in mitotic A549 cells but not in A549-T24 cells (Fig. 3E(a), Fig. 3E(b) and Fig. 3E(d)), implying that the downregulation of USP24 may induce autophagy to maintain genomic stability in drug-sensitive lung cancer cells but not in drug-resistant lung cancer cells (Fig. 3E)."
| 1

reach
"Increased levels of USP24 lead to p53 stabilization and upregulation and subsequent activation of p53 downstream targets.can be reversed by the action of DUBs.Thus, targeting DUBs may have promising potential in cancer therapy.9 USP24offers novel exciting perspectives in basicand translational research, and anapoptosis and genome stability."
USP24 affects GPR31
| 3
| 3

sparser
"The interaction of GPR31 with USP24 was increased in H520OV− L1−FGGY cells (Fig.  xref L) by using an immunoprecipitation assay, whereas, the total USP24 protein level in cell lysates did not change."

sparser
"Collectively, these findings implied that the L1-FGGY reduces the expression of FGGY, thereby, facilitating the binding of more USP24 to GPR31 that increases GPR31 de-ubiquitination and activation of metabolic pathway for AA/12-LOX/12S-HETE and the subsequent signaling through the Wnt pathway in tumor cells for enhanced cell proliferation and invasion (Figure S xref )."

sparser
"Then we detected binding of USP24 with FGGY or GPR31 (Fig.  xref J), illustrating the physical interaction of USP24 with either FGGY or GPR31, whereby, the ubiquitination of GPR31 was increased by USP24 knockdown (Fig.  xref K) to indicate that USP24 acts as a deubiquitination enzyme of GPR31."
USP24 affects DPYSL2
| 3
USP24 inhibits DPYSL2.
| 2
USP24 inhibits DPYSL2. 2 / 2
| 2

reach
"Previous study have shown that USP24 inhibits the progression of neuroblastoma by stabilizing CRMP2 [27]."

reach
"USP24 was found to inhibit the progression of neuroblastoma by stabilizing CRMP2 ."
USP24 deubiquitinates DPYSL2.
| 1
USP24 deubiquitinates DPYSL2. 1 / 1
| 1

reach
"Deubiquitination of CRMP2 by USP24 ensured proper spindle pole assembly and blocked chromosomal instability and aneuploidy observed upon USP24 knockdown in NBT [173]."
USP24 affects BMX
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
TRAF6 affects USP24
| 1 2
| 1 2

sparser
"The interaction between USP24 and TRAF6 in mitosis was studied (Fig.  xref )."

reach
"The interaction between USP24 and TRAF6 in mitosis was studied (Fig. 3F)."

sparser
"The data indicated that USP24 can interact with TRAF6 in mitotic A549 cells (Fig.  xref )."
TLR9 affects USP24
| 3
TLR9 increases the amount of USP24.
| 2
TLR9 increases the amount of USP24. 2 / 2
| 2

reach
"The expression level of USP24 was inversely correlated with miR-139-5p expression in SSc patients and induced by TLR9 stimulation in healthy pDCs."

reach
"The expression level of USP24 is inversely correlated with miR-139-5p expression in SSC patients and is induced by TLR9 stimulation in healthy pDCs [ 63 ]."
TLR9 inhibits USP24.
| 1
TLR9 inhibits USP24. 1 / 1
| 1

reach
"The study also demonstrated that USP24 is down-regulated by TLR9 stimulation, but the underlying mechanism of the TLR9-USP24-IFN axis is unclear and requires further investigation [ 63 ]."
STAT3 affects USP24
| 3
STAT3 activates USP24.
| 2
STAT3 activates USP24. 2 / 2
| 2

reach
"IL-6 in the TME drives nuclear factor κB (NF-κB)/signal transducers and activators of transcription 3 (STAT3) signaling to transcriptionally increase USP24."

reach
"Deubiquitinase USP24 activated by IL-6/STAT3 enhances PD-1 protein stability and suppresses T cell antitumor response."
STAT3 increases the amount of USP24.
| 1
STAT3 increases the amount of USP24. 1 / 1
| 1

reach
"Overexpression of NF-κB and STAT3, but not E2F and SP1, up-regulated USP24 mRNA expression in Jurkat T cells (Fig. 4I)."
RP2 affects USP24
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
RHOJ affects USP24
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
PTTG1 affects USP24
| 1 2
| 1 2

sparser
"It was discovered that USP24 interacts with Securin."

reach
"Here we found that USP24 interacted with securin in mitotic cells (XREF_FIG; XREF_SUPPLEMENTARY)."

sparser
"Here we found that USP24 interacted with securin in mitotic cells ( xref ; xref )."
ORF10 affects USP24
1 1 | 1
ORF10 binds USP24.
1 1 |
1 1 |

No evidence text available

No evidence text available
ORF10 activates USP24.
| 1
ORF10 activates USP24. 1 / 1
| 1

eidos
"In the presence of etoposide , USP24 was no longer induced by KSHV ORF10 , as it was deleted , and p53 levels were reduced in KSHV orf10 expressing as compared to untransfected control ."
N affects USP24
3 |
N binds USP24. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
| DOI
LYPD3 affects USP24
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
KRAS affects USP24
| 3
KRAS-G12D decreases the amount of USP24. 3 / 3
| 3

reach
"Taken together, these data revealed that Kras G12D - or EGFR L858R -mediated pathways negatively regulated USP24 expression, which might trigger lung cancer formation."

reach
"Based on the data in this study and our previous study, we propose that EGFR L858R - and Kras G12D -mediated signaling pathways inhibit USP24 expression, which is beneficial for cancer formation during the early stage."

reach
"XREF_BIBR, XREF_BIBR Although their levels were increased in some specimens from late-stage lung cancer patients, inhibition of Kras G12D - or EGFR L858R -mediated activities in all cell lines at different stages increased the USP24 level."
HIF1AN affects USP24
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3 |

No evidence text available

No evidence text available

No evidence text available
GPR31 affects USP24
| 3
| 3

sparser
"The interaction of GPR31 with USP24 was increased in H520OV− L1−FGGY cells (Fig.  xref L) by using an immunoprecipitation assay, whereas, the total USP24 protein level in cell lysates did not change."

sparser
"Collectively, these findings implied that the L1-FGGY reduces the expression of FGGY, thereby, facilitating the binding of more USP24 to GPR31 that increases GPR31 de-ubiquitination and activation of metabolic pathway for AA/12-LOX/12S-HETE and the subsequent signaling through the Wnt pathway in tumor cells for enhanced cell proliferation and invasion (Figure S xref )."

sparser
"Then we detected binding of USP24 with FGGY or GPR31 (Fig.  xref J), illustrating the physical interaction of USP24 with either FGGY or GPR31, whereby, the ubiquitination of GPR31 was increased by USP24 knockdown (Fig.  xref K) to indicate that USP24 acts as a deubiquitination enzyme of GPR31."
BTRC affects USP24
| 2 1
BTRC binds USP24.
| 1 1
| 1 1

reach
"Immunoprecipitation analysis confirmed the interaction between USP24 and beta-TrCP, and ubiquitinated beta-TrCP was significantly increased in USP24-knockdown A549 cells."

sparser
"Immunoprecipitation analysis confirmed the interaction between USP24 and β-TrCP (Fig.  xref ), and ubiquitinated β-TrCP was significantly increased in USP24-knockdown A549 cells (Fig.  xref and Supplementary Fig.  xref )."
BTRC activates USP24.
| 1
BTRC activates USP24. 1 / 1
| 1

reach
"In addition, beta-TrCP overexpression can abolish the effect of USP24 knockdown in IL-6 expression."
BMX affects USP24
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
1 | 1
Zoledronic acid decreases the amount of USP24. 2 / 2
1 | 1

No evidence text available

reach
"In particular, we found that ZOL inhibited expression of the ubiquitin-specific peptidase 24 ( USP24) a de-ubiquitin protease that is overexpressed in bortezomib-resistant RPMI-R5 cells compared to RP[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
2 |
Valproic acid decreases the amount of USP24. 2 / 2
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No evidence text available

No evidence text available
Paracetamol affects USP24
2 |
Paracetamol decreases the amount of USP24. 2 / 2
2 |

No evidence text available

No evidence text available
2 |
Manganese atom increases the amount of USP24.
1 |
Manganese atom increases the amount of USP24. 1 / 1
1 |

No evidence text available
Manganese atom decreases the amount of USP24.
1 |
Manganese atom decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Cisplatin affects USP24
2 |
Cisplatin decreases the amount of USP24. 2 / 2
2 |

No evidence text available

No evidence text available
2 |

No evidence text available

No evidence text available
2 |
Arsenic atom increases the amount of USP24.
1 |
Arsenic atom increases the amount of USP24. 1 / 1
1 |

No evidence text available
Arsenic atom decreases the amount of USP24.
1 |
Arsenic atom decreases the amount of USP24. 1 / 1
1 |

No evidence text available
ZRANB1 affects USP24
2 |
2 |

No evidence text available

No evidence text available
WWP2 affects USP24
2 |
2 |

No evidence text available

No evidence text available
VIPR2 affects USP24
2 |
2 |

No evidence text available

No evidence text available

reach
"This third, independent assay format thus supports a direct interaction between USP24, p53 and orf10."

reach
"KSHV orf10 interacted with the deubiquitinase USP24 and with p53 itself, and p53 is a known substrate for USP24 [XREF_BIBR]."
| 2
| 1

reach
"In this study, we revealed a new biological function of USP24, that is, USP24 can restrict the innate antiviral immune response and downregulate interferon production."
USP24 activates immune response.
| 1

reach
"These results indicated that functional depletion of USP24 attenuates tumorigenesis in TetO-Egfr mice, supporting its oncogenic role.Next, we explored whether USP24 inactivation promotes antitumor immunity."
USP24 affects cell cycle
| 2
| 2

reach
"Overexpression of E2F4 in USP24 silenced cells rescued only the E2F1 mRNA level and partially abolished the effect of the USP24 knockdown on the G1-S transition and colony formation (XREF_FIG; XREF_SUPPLEMENTARY), implying that E2F4 might be involved in USP24 mediated cell cycle progression."

reach
"Thus, E2F4 may be involved in USP24-mediated cell cycle progression."
USP24 affects activation IFNbeta
| 2
USP24 inhibits activation IFNbeta. 2 / 2
| 2

eidos
"Moreover , USP2-4 , but not USP2-1 , attenuated the activation of IFNbeta promoter after SeV infection , indicating a role for USP2 in suppressing viral immunity [ 155 ] ."

eidos
"USP2-4 also inhibited the activation of the IFNbeta promoter by modulating retinoic acid-inducible gene-I ( RIG-I ) , melanoma differentiation-associated gene 5 ( MDA5 ) , mitochondrial antiviral signaling protein ( MAVS ) , TANK binding kinase 1 ( TBK1 ) , and possibly activating interferon regulatory factor 3 ( IRF3 ) [ 155 ] ."
USP24 affects ZRANB1
2 |
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No evidence text available

No evidence text available
USP24 affects WWP2
2 |
2 |

No evidence text available

No evidence text available
USP24 affects VIPR2
2 |
2 |

No evidence text available

No evidence text available

reach
"This third, independent assay format thus supports a direct interaction between USP24, p53 and orf10."

reach
"KSHV orf10 interacted with the deubiquitinase USP24 and with p53 itself, and p53 is a known substrate for USP24 [XREF_BIBR]."
USP24 affects USP9X
| 1 1
USP24 increases the amount of USP9X.
| 1
USP24 increases the amount of USP9X. 1 / 1
| 1

reach
"Interestingly, knockdown of USP24 in Jurkat cells significantly induced cell death and reduced Mcl-1 but not USP9X protein level."
USP24 binds USP9X.
| 1
| 1

sparser
"Indeed, our pull-down studies for NOTCH1, followed by detection of USP24 and USP9x in western blot experiments, showed that neither USP9x nor USP24 interact with NOTCH1 ( xref )."
USP24 affects SMARCA4
1 | 1
1 |

No evidence text available
| 1

sparser
"Next, when HA-BRD7 and HA-BRG1 were expressed in H1299 cells or MEFs, we found that USP24 interacted with BRD7 and BRG1, indicating that USP24 could interact with more than one BRD-containing protein in different cell lines (Fig.  xref B,C and Suppl."
USP24 affects Proteasome
| 2
USP24 deubiquitinates Proteasome.
| 1
USP24 leads to the deubiquitination of Proteasome. 1 / 1
| 1

reach
"The ubiquitin hydrolase USP24 directly interacts with p300 protein, and reduces p300 protein ubiquitination and proteasome-mediated degradation, leading to p300 protein up-regulation and histone acetylation 15 (Figure 1C)."
USP24 binds Proteasome.
| 1

reach
"The ubiquitin hydrolase USP24 directly interacts with p300 protein, and reduces p300 protein ubiquitination and proteasome-mediated degradation, leading to p300 protein up-regulation and histone acetylation 15 (Figure 1C)."
USP24 affects PUL38
| 2
USP24 binds PUL38. 2 / 2
| 2

reach
"This interaction between USP24 and PUL38 plays a crucial role in the replication of HCMV."

reach
"Comparing cells infected with wild-type HCMV to those lacking PUL38, it was observed that PUL38 binds to USP24 in wild-type HCMV-infected cells, thereby inhibiting its ability to stabilize NCOA4."
USP24 affects PRKACA
| 2
| 2

sparser
"Molecular mechanisms and the interaction between USP24 and PKA-Cα were studied with co-immunoprecipitation."

sparser
"In summary, USP24 not only interacts with PKA-Cα to positively regulate each other, increasing CREB1 phosphorylation but also stabilizes p300 and increases the transcriptional activity of p-CREB during adipogenesis."
USP24 affects ORF10
1 1 |
1 1 |

No evidence text available

No evidence text available
| 2

reach
"Previous study have shown that USP24 inhibits the progression of neuroblastoma by stabilizing CRMP2 [27]."

reach
"USP24 was found to inhibit the progression of neuroblastoma by stabilizing CRMP2 ."
USP24 affects NOTCH1
| 1 1
USP24 inhibits NOTCH1.
| 1
USP24 inhibits NOTCH1. 1 / 1
| 1

reach
"36 Besides, Zhi et al. revealed that the knockdown of ubiquitin‐specific protease 24 (USP24) reduced the stability of PLK1 and consequently decreased the activity of NOTCH1, resulting in alleviating tumor progression."
USP24 binds NOTCH1.
| 1
| 1

sparser
"Indeed, our pull-down studies for NOTCH1, followed by detection of USP24 and USP9x in western blot experiments, showed that neither USP9x nor USP24 interact with NOTCH1 ( xref )."
USP24 affects NCS1
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No evidence text available

No evidence text available
USP24 affects MDM2
| 2
USP24 increases the amount of MDM2. 2 / 2
| 2

reach
"XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR Our recent study showed that USP24 variants from SNPs and RNA editing products increased the levels of USP24 and MDM2, which regulates Suv39h1 in lung cancer cells, subsequently resulting in an increase in metastatic activities during lung cancer progression."

reach
"Intriguingly, USP24 up-regulates the expression of MDM2, the well-known E3 ligase of P53, in lung cancer, which contradicts the above finding 31."
USP24 affects Ku-70
| 2
| 2

sparser
"The data revealed that USP24 interacted with Ku70 ( xref )."

sparser
"Previous studies indicated that Ku70 was involved in Bax mitochondrial translocation. xref Herein, we used a yeast two-hybrid assay to study the interaction between Ku70 and USP24 ( xref ; xref )."
USP24 affects GSDMB protein bladder cancer
| 2
USP24 activates GSDMB protein bladder cancer. 2 / 2
| 2

eidos
"USP24 interacts with GSDMB to up-regulate the GSDMB protein level in bladder cancer After revealing the important role of GSDMB in bladder cancer , we studied how GSDMB was regulated in bladder cancer for the targeted treatment of bladder cancer ."

eidos
"To sum up , USP24 interacts with GSDMB to up-regulate the GSDMB protein level in bladder cancer ."
USP24 affects Flag
| 2
| 2

sparser
"Our results also showed that, after HEK293T cells were transfected with increasing amounts of the Flag-USP24 plasmid, the expression of YAP1 increased in a dose-dependent manner (Fig.  xref F)."

sparser
"Moreover, a cycloheximide (CHX) chase experiment indicated that the co-expression of Flag-USP24 could significantly prolong the half-life of exogenous Myc-tagged YAP1 compared to the negative control (Fig.  xref G)."
USP24 affects FTH1
| 2
USP24 activates FTH1. 2 / 2
| 2

reach
"Knockdown of USP24 decreased NCOA4 protein stability and ferritin heavy chain degradation in lysosomes."

reach
"USP24 knockdown decreased NCOA4 stability and FTH1 degradation."
USP24 affects ERK
| 1 1
| 1 1

sparser
"Further study indicated that USP24 could interact with Erk1/2 and p-Erk1/2 ( xref ), suggesting that Erk1/2 might be the kinase for phosphorylation of USP24 at S2604 to decrease the USP24 level."

reach
"Further study indicated that USP24 could interact with Erk1/2 and p-Erk1/2 (XREF_FIG), suggesting that Erk1/2 might be the kinase for phosphorylation of USP24 at S2604 to decrease the USP24 level."
USP24 affects E2F1
| 2
USP24 increases the amount of E2F1.
| 1
USP24 increases the amount of E2F1. 1 / 1
| 1

reach
"Knockdown of USP24 increased the CCNA2 and E2F1 mRNA levels (XREF_FIG); the same results were obtained in the cDNA array performed under USP24 knockdown conditions (XREF_SUPPLEMENTARY)."
USP24 decreases the amount of E2F1.
| 1
USP24 decreases the amount of E2F1. 1 / 1
| 1

reach
"However, the overexpression of E2F4 following USP24 knockdown elevates E2F1 mRNA levels and partially reduces the G1-S transition."

reach
"USP24 promotes hepatocellular carcinoma tumorigenesis through deubiquitinating and stabilizing TRAF2."

reach
"USP24 promotes autophagy-dependent ferroptosis in hepatocellular carcinoma by reducing the K48-linked ubiquitination of Beclin1."
USP24 affects CPNE2
2 |
2 |

No evidence text available

No evidence text available
USP24 affects CHRM4
2 |
2 |

No evidence text available

No evidence text available
USP24 affects CCNB1
| 2
Modified USP24 increases the amount of CCNB1. 1 / 1
| 1

reach
"Overexpression of GFP-USP24 increased the cyclin B1 level, suggesting that USP24 was beneficial for cells that remained in mitosis (XREF_SUPPLEMENTARY)."
USP24 increases the amount of CCNB1. 1 / 1
| 1

reach
"Knockdown of USP24 increased the CDK1 and cyclin B1 levels, and increased the ratio of cells in the G2/M stage, indicating that USP24 reduction was beneficial for cell cycle progression (XREF_SUPPLEMENTARY)."
USP24 affects BCAR1
| 2
USP24 activates BCAR1. 2 / 2
| 2

reach
"Taken together, our results demonstrated that USP24 knockdown decreased E2F4, p130 and TFDP1, resulting in an increase in the E2F1 levels and a subsequent increase in the G1-S transition, leading to tumorigenesis."

reach
"Further experiments reveal that USP24 knockdown decreases the stability of E2F4 protein, p130, and TFDP1, resulting in an increase in E2F1 levels and a promotion of the G1-S transition, ultimately lea[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP24 affects Aneuploidy
| 2
| 2

eidos
"We have found that reduced expression of USP24 leads to abnormalities in the mitotic spindle and a significant increase in chromosome missegregation and aneuploidy in a mouse model ."

eidos
"USP24 prevented aneuploidy by maintaining spindle-associated CRMP2 , which is required for mitotic accuracy ."
USP24 affects ABCG2
| 2
USP24 inhibits ABCG2.
| 1
USP24 inhibits ABCG2. 1 / 1
| 1

reach
"In summary, USP24-i targeting USP24 activates autophagy to promote the degradation of ABCG2 and PD-L1, leading to the inhibition of drug resistance acquired from lung cancer therapy."
USP24 decreases the amount of ABCG2.
| 1
USP24 decreases the amount of ABCG2. 1 / 1
| 1

reach
"Targeting USP24 by USP24-i-101 induces autophagy in interphase and mitosis by inhibiting E2F4 and TRAF6, respectively, thereby inhibiting the expression of ABCG2 and PD-L1 and maintaining genomic integrity, resulting in the inhibition of drug resistance acquired by gefitinib- or Taxol-treatment of lung cancer (Fig. 8D)."
SUV39H1 affects USP24
2 |
2 |

No evidence text available

No evidence text available
SMARCA4 affects USP24
1 | 1
1 |

No evidence text available
| 1

sparser
"Next, when HA-BRD7 and HA-BRG1 were expressed in H1299 cells or MEFs, we found that USP24 interacted with BRD7 and BRG1, indicating that USP24 could interact with more than one BRD-containing protein in different cell lines (Fig.  xref B,C and Suppl."
Particulate Matter increases the amount of USP24. 2 / 2
2 |

No evidence text available

No evidence text available
| 2
Parkinson Disease increases the amount of USP24.
| 1
Parkinson Disease increases the amount of USP24. 1 / 1
| 1

reach
"Thus, PD associated USP24 SNPs could similarly increase USP24 expression, leading to lowering of autophagy flux."

reach
"However, the association between USP24 and PD is still controversial based on several association studies, which necessitates further investigation of this DUB."
PUL38 affects USP24
| 2
USP24 binds PUL38. 2 / 2
| 2

reach
"This interaction between USP24 and PUL38 plays a crucial role in the replication of HCMV."

reach
"Comparing cells infected with wild-type HCMV to those lacking PUL38, it was observed that PUL38 binds to USP24 in wild-type HCMV-infected cells, thereby inhibiting its ability to stabilize NCOA4."
PRKACA affects USP24
| 2
| 2

sparser
"Molecular mechanisms and the interaction between USP24 and PKA-Cα were studied with co-immunoprecipitation."

sparser
"In summary, USP24 not only interacts with PKA-Cα to positively regulate each other, increasing CREB1 phosphorylation but also stabilizes p300 and increases the transcriptional activity of p-CREB during adipogenesis."
PLK1 affects USP24
| 2
| 2

sparser
"HEK293T cells were co‐transfected with HA‐USP24 and Flag‐PLK1, and immunoprecipitation assay showed that USP24 and PLK1 interacted (Figure  xref )."

sparser
"USP24 interacts with PLK1."
NCS1 affects USP24
2 |
2 |

No evidence text available

No evidence text available
Ku-70 affects USP24
| 2
| 2

sparser
"The data revealed that USP24 interacted with Ku70 ( xref )."

sparser
"Previous studies indicated that Ku70 was involved in Bax mitochondrial translocation. xref Herein, we used a yeast two-hybrid assay to study the interaction between Ku70 and USP24 ( xref ; xref )."
HYCC1 affects USP24
| 2
HYCC1 activates USP24. 2 / 2
| 2

reach
"We identified that USP24 drives HCC progression by stabilizing YAP1 through direct interaction and deubiquitination, leading to constitutive activation of YAP1 downstream targets."

reach
"Moreover, the transwell experiment was conducted to assess the migration capacity, and the results demonstrated that the migration capacity of HCC cells was increased by knocking down USP24 (Fig. 2G, H)."
Flag affects USP24
| 2
| 2

sparser
"Our results also showed that, after HEK293T cells were transfected with increasing amounts of the Flag-USP24 plasmid, the expression of YAP1 increased in a dose-dependent manner (Fig.  xref F)."

sparser
"Moreover, a cycloheximide (CHX) chase experiment indicated that the co-expression of Flag-USP24 could significantly prolong the half-life of exogenous Myc-tagged YAP1 compared to the negative control (Fig.  xref G)."
ERK affects USP24
| 1 1
| 1 1

sparser
"Further study indicated that USP24 could interact with Erk1/2 and p-Erk1/2 ( xref ), suggesting that Erk1/2 might be the kinase for phosphorylation of USP24 at S2604 to decrease the USP24 level."

reach
"Further study indicated that USP24 could interact with Erk1/2 and p-Erk1/2 (XREF_FIG), suggesting that Erk1/2 might be the kinase for phosphorylation of USP24 at S2604 to decrease the USP24 level."
EGF affects USP24
| 2
EGF phosphorylates USP24. 2 / 2
| 2

reach
"In this study, we found that the phosphorylation of USP24 by CDK1- or EGF mediated kinase activity decreased the USP24 level."

reach
"In addition to the phosphorylation of USP24 in mitosis, USP24 is also phosphorylated by EGF activated Erk1/2 kinase activity to turnover USP24."
CPNE2 affects USP24
2 |
2 |

No evidence text available

No evidence text available
CHRM4 affects USP24
2 |
2 |

No evidence text available

No evidence text available
BAX affects USP24
| 1 1
| 1 1

sparser
"Next, we found that USP24 interacted with Bax ( xref D)."

reach
"Next, we found that USP24 interacted with Bax (XREF_FIG D)."
1,2-dimethylhydrazine increases the amount of USP24. 2 / 2
2 |

No evidence text available

No evidence text available
4,4'-sulfonyldiphenol increases the amount of USP24.
1 |
4,4'-sulfonyldiphenol increases the amount of USP24. 1 / 1
1 |

No evidence text available
4,4'-sulfonyldiphenol decreases the amount of USP24.
1 |
4,4'-sulfonyldiphenol decreases the amount of USP24. 1 / 1
1 |

No evidence text available
ΜM. affects USP24
| 1
ΜM. inhibits USP24. 1 / 1
| 1

reach
"The IC50 of G9 is reported to be 1 μM. G9 inhibits the function of Usp9x and Usp24 by creating a covalent, gradually reversible connection with cysteine residues."
Α-IL-6 affects USP24
| 1
Α-IL-6 activates USP24. 1 / 1
| 1

reach
"The potential of α-IL-6 for enhancing T cell function warrants further investigation.In conclusion, we reveal that IL-6/STAT3/NF-κB transcriptionally activates USP24 to drive USP24-mediated deubiquitinating machinery to stabilize PD-1 and suppress T cell activity."
1 |
Troglitazone decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Triptonide affects USP24
1 |
Triptonide decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Trichostatin A increases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Sodium fluoride increases the amount of USP24. 1 / 1
1 |

No evidence text available
Saxitoxin affects USP24
1 |
Saxitoxin increases the amount of USP24. 1 / 1
1 |

No evidence text available
Rep affects USP24
1 |
1 |

No evidence text available
Protein pUL38 affects USP24
| 1
Protein pUL38 binds USP24. 1 / 1
| 1

reach
"In virus infection, human cytomegalovirus protein pUL38 binds to host protein ubiquitin-specific protease 24 (USP24), inhibiting NCOA4-mediated ferritinophagy against host fibroblasts cell death [60]."
Potassium chromate decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Phlorizin affects USP24
1 |
Phlorizin decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |

No evidence text available
Patients tumors affects USP24
| 1
Patients tumors inhibits USP24. 1 / 1
| 1

eidos
"We further found that patients whose tumors had reduced expression of USP24 , defined as less than the 20th percentile , were significantly more likely to suffer disease progression and to die of any cause ."
PUL38 protein affects USP24
| 1
PUL38 protein binds USP24. 1 / 1
| 1

reach
"For example, the pUL38 protein of HCMV binds to USP24 to antagonize the cellular stress response, thereby preventing premature cell death [204]."
Oxaliplatin affects USP24
1 |
Oxaliplatin decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Nickel atom affects USP24
1 |
Nickel atom increases the amount of USP24. 1 / 1
1 |

No evidence text available
Mono(2-ethylhexyl) phthalate decreases the amount of USP24. 1 / 1
1 |

No evidence text available
MiR-363 affects USP24
| 1
MiR-363 activates USP24. 1 / 1
| 1

reach
"It is noteworthy that miR-25 targeting BMPR2 and IRS1, miR-363 targeting USP24, miR-28 targeting HECW2 and miR-210 targeting ATP5I, ME3, MTCH1 and CPT2 were highly associated with slow-twitch oxidative fibers, fast-twitch oxidative fibers, ADP and ATP concentration suggesting an essential role of the miRNA mRNA regulatory networking in modulating the mitochondrial energy expenditure in the porcine muscle."
MiR-21-5p affects USP24
| 1
MiR-21-5p increases the amount of USP24. 1 / 1
| 1

reach
"Taken together, these results indicated that knockdown of miR-21-5p can inhibit the expression of the deubiquitinase USP24, thereby reducing the levels of SIRT7 and autophagy and reversing sorafenib r[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
Methylmercury chloride decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Methidathion decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Methapyrilene decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Manganese(II) chloride increases the amount of USP24. 1 / 1
1 |

No evidence text available
Jinfukang affects USP24
1 |
Jinfukang decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Ivermectin affects USP24
1 |
Ivermectin decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Irinotecan affects USP24
1 |
Irinotecan decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Indometacin affects USP24
1 |
Indometacin decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-1226-3p decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Ginger extract increases the amount of USP24. 1 / 1
1 |

No evidence text available
Gefitinib affects USP24
| 1
Gefitinib increases the amount of USP24. 1 / 1
| 1

reach
"Gefitinib and FTI-276, which inhibited the EGFR and Kras activities 21 in the A431 and A549 cell lines, respectively, increased USP24 expression (XREF_FIG)."
| 1

reach
"Erastin was then used to induce ferroptosis, and it was found that cell death was more visible in the USP24 overexpressed group, which can be partially reversed by Fer-1 (Fig. 7B)."
Fenthion affects USP24
1 |
Fenthion decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Factor 2 affects USP24
| 1
USP24 binds factor 2. 1 / 1
| 1

sparser
"Mechanistically, USP24 binds to tumor necrosis factor receptor-associated factor 2(TRAF2) and inhibits its degradation, thereby promoting the accumulation of TRAF2."
Ethanol affects USP24
1 |
Ethanol decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Doxycycline affects USP24
| 1
| 1

reach
"Doxycycline treatment dramatically increased the signal in the lungs of the wild-type and USP24 knockout mice, suggesting that doxycycline successfully induced tumors in the lung of the EGFR *USP24 (#2 and #3 mice) and EGFR *USP24 (#8 and #9 mice) mice."
1 |
Dexamethasone decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Deoxynivalenol phosphorylates USP24. 1 / 1
1 |

No evidence text available
1 |
Deltamethrin increases the amount of USP24. 1 / 1
1 |

No evidence text available
Degrasyn affects USP24
| 1
| 1

eidos
"Peterson and colleagues suggested that dual inhibition of USP9X and USP24 by WP1130 provides greater anti-myeloma activity ."
Decabromodiphenyl ether decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
Cyclosporin A increases the amount of USP24. 1 / 1
1 |

No evidence text available
Certain affects USP24
| 1
Certain activates USP24. 1 / 1
| 1

eidos
"Therefore , upregulation of USP2-4 by certain stimuli may alter the contraction of cardiac muscle ."
1 |
Cefaloridine decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Cadmium dichloride increases the amount of USP24. 1 / 1
1 |

No evidence text available
Butanal affects USP24
1 |
Butanal decreases the amount of USP24. 1 / 1
1 |

No evidence text available
Bisphenol F affects USP24
1 |
Bisphenol F decreases the amount of USP24. 1 / 1
1 |

No evidence text available
1 |
1 |

No evidence text available
Auramine O affects USP24
1 |
Auramine O increases the amount of USP24. 1 / 1
1 |

No evidence text available
Aristolochic acid A decreases the amount of USP24. 1 / 1
1 |

No evidence text available
ZEB1 affects USP24
1 |
1 |

No evidence text available
YWHAG affects USP24
1 |
1 |

No evidence text available
WWTR1 affects USP24
1 |
1 |

No evidence text available
WWOX affects USP24
1 |
1 |

No evidence text available
USP9X affects NOTCH1
| 1
| 1

sparser
"Indeed, our pull-down studies for NOTCH1, followed by detection of USP24 and USP9x in western blot experiments, showed that neither USP9x nor USP24 interact with NOTCH1 ( xref )."
USP24 affects γ-H2AX
| 1
USP24 increases the amount of γ-H2AX. 1 / 1
| 1

reach
"The data indicated that knockdown of USP24 significantly decreased the levels of γ-H2AX in cells with irradiation, suggesting that loss of USP24 results in a higher DNA damage repair activity in response to the same DNA damage repair by irradiation (Fig. 4B)."
| 1

reach
"The results indicated that USP24 overexpression decreased wound-healing ability (Fig. 5F and Supplementary Fig. 3A, B) and inhibited the migration of HCC cells (Fig. 5G, H)."
USP24 affects whole-chromosome aneuploidy
| 1
USP24 inhibits whole-chromosome aneuploidy. 1 / 1
| 1

eidos
"At the molecular level , USP24 loss leads to destabilization of the microtubule assembly factor CRMP2 - producing mitotic errors and leading to chromosome missegregation and whole-chromosome aneuploidy ."
USP24 affects ubiquitination RUNX2
| 1
USP24 inhibits ubiquitination RUNX2. 1 / 1
| 1

eidos
"However , unlike HAUSP , USP24 was unable to interact with RUNX2 and reduce ubiquitination levels of RUNX2 ( Supplementary Fig.12 ) , suggesting that HAUSP , not USP24 , functions as a bona-fide RUNX2-DUB ."
USP24 affects surface Cav1.2
| 1
USP24 inhibits surface Cav1.2. 1 / 1
| 1

eidos
"An overexpression study using kidney cells demonstrated that USP2-4 decreased the surface expression of Cav1.2 and attenuated Cav currents by suppression of ubiquitination of Cav1.2 and alpha2delta-1 subunits [ 135 ] ."
USP24 affects sodium distribution body
| 1
USP24 activates sodium distribution body. 1 / 1
| 1

eidos
"The significant regulatory roles of USP2 on ENaC and MR expression suggest that USP2-4 is a key modulator for sodium distribution in the body , and therefore for blood pressure ."
USP24 affects rep
1 |
1 |

No evidence text available
USP24 affects proteolytic cleavage extracellular loop alphaENaC
| 1
USP24 activates proteolytic cleavage extracellular loop alphaENaC. 1 / 1
| 1

eidos
"Additionally , USP2-4 also contributed to the proteolytic cleavage of the extracellular loop of alphaENaC , leading to ENaC activation [ 95,97 ] ."
USP24 affects protein pUL38
| 1
Protein pUL38 binds USP24. 1 / 1
| 1

reach
"In virus infection, human cytomegalovirus protein pUL38 binds to host protein ubiquitin-specific protease 24 (USP24), inhibiting NCOA4-mediated ferritinophagy against host fibroblasts cell death [60]."
USP24 affects protein beta-TrCP p300
| 1
USP24 activates protein beta-TrCP p300. 1 / 1
| 1

eidos
"Up-regulated USP24 increased the protein level of beta-TrCP and p300 to promote lung cancer malignancy 31 ."
USP24 affects process
| 1
USP24 activates process. 1 / 1
| 1

eidos
"We further showed that USP24 inhibitors could block this process via inducing GSDMB degradation in cancer cells , which provided a therapeutic strategy for inhibiting the GSDMB / STAT3 axis in bladder cancer ."
USP24 affects pathway
| 1
USP24 activates pathway. 1 / 1
| 1

sparser
"Ubiquitin‐specific peptidase 24 (USP24) is a member of the DUB family and contains a ubiquitin related domain (UBA) and a ubiquitin C‐terminal hydrolase (UCH) domain, which is the catalytic domain. xref USP24 is rarely reported, but it plays an important regulatory role in tumors. xref , xref It has been reported that USP24 increases interleukin‐6 transcription in M2 macrophages and lung cancer cells in lung cancer. xref USP24 increases the level and stability of most BRD‐containing proteins by removing ubiquitin from BRD Lys391/Lys400. xref In bladder cancer, USP24 activates the STAT3 pathway by stabilizing GSDMB and promotes the growth and invasion ability of bladder cancer cells. xref However, the mechanism of USP24 in gastric cancer remains unclear."
USP24 affects pUL38 protein
| 1
PUL38 protein binds USP24. 1 / 1
| 1

reach
"For example, the pUL38 protein of HCMV binds to USP24 to antagonize the cellular stress response, thereby preventing premature cell death [204]."
USP24 affects mouse model
| 1
USP24 inhibits mouse model. 1 / 1
| 1

eidos
"We have found that reduced expression of USP24 leads to abnormalities in the mitotic spindle and a significant increase in chromosome missegregation and aneuploidy in a mouse model ."
USP24 affects miR-21-5p
| 1
USP24 inhibits miR-21-5p. 1 / 1
| 1

reach
"The TUNEL results showed that the miR-21-5p inhibitor increased apoptosis and that overexpression of USP24 rescued the influence of miR-21-5p inhibition ( Fig. 6 D)."
USP24 affects metastasis breast cancer
| 1
USP24 activates metastasis breast cancer. 1 / 1
| 1

eidos
"USP24 also enhances TGFbeta-induced EMT and metastasis of breast cancer [ 61 ] ."
USP24 affects lysosomal
| 1
USP24 inhibits lysosomal. 1 / 1
| 1

reach
"Blocking lysosomal function with bafilomycin led to significant increase in accumulation of GFP-LC3 positive autophagosomes in USP24 knockdown cells, indicating that USP24 is negatively regulating autophagy flux without affecting lysosomal degradation)."
| 1
| 1

reach
"The localization of Bax in cells expressing GFP-USP24 indicated that USP24 expression increased Bax 's mitochondrial localization, implying that USP24 induced cell apoptosis (XREF_FIG)."
USP24 affects interphase
| 1
| 1

reach
"In this study, we found that targeting USP24 by the specific USP24 inhibitors, USP24-i and its analogues, dramatically activated autophagy in the interphase and mitotic periods of lung cancer cells by inhibiting E2F4 and TRAF6, respectively."
USP24 affects glutathione
| 1
| 1

reach
"The results indicated that overexpression of USP24 decreased GSH while increased the level of MDA in HCC cells (Fig. 7H, I and Supplementary Fig. 4G, H), further confirming that USP24 promotes ferroptosis in HCC cells."
USP24 affects formation
| 1
USP24 inhibits formation. 1 / 1
| 1

sparser
"To investigate the molecular mechanism by which USP24 inhibited lung cancer formation, H1299 cells with GFP-USP24 or GFP expression were recorded using time-lapse microscopy ( xref ; xref )."
USP24 affects ferritin
| 1
| 1

reach
"pUL38 blocked USP24 mediated ferritin degradation in lysosomes, which could otherwise be detrimental to the lysosome and initiate cell death."
USP24 affects factor 2
| 1
USP24 binds factor 2. 1 / 1
| 1

sparser
"Mechanistically, USP24 binds to tumor necrosis factor receptor-associated factor 2(TRAF2) and inhibits its degradation, thereby promoting the accumulation of TRAF2."

reach
"Besides, USP24 promotes the immune evasion of EV71 by restricting the K63-linked polyubiquitination of TBK1(Zang et al., 2023)."
USP24 affects deubiquitination GSDMB
| 1
USP24 activates deubiquitination GSDMB. 1 / 1
| 1

eidos
"Thus , USP24 may mediate the deubiquitination of GSDMB and maintain its stability ."

reach
"In this study, we revealed a new biological function of USP24, that is, USP24 can restrict the innate antiviral immune response and downregulate interferon production."
USP24 affects cyclin B1
| 1
USP24 phosphorylates cyclin B1. 1 / 1
| 1

rlimsp
"Samples were analyzed with antibodies against USP24 phosphorylation at indicated residues, cyclin B1 and actin."
USP24 affects circadian rhythm calcium permeability
| 1
USP24 activates circadian rhythm calcium permeability. 1 / 1
| 1

eidos
"Together , these results suggest that USP2-4 modulates the circadian rhythm of calcium permeability in the intestine via posttranslational control of NHERF4 at the plasma membrane ."

reach
"Herein, we also found that GFP-USP24 overexpression in mitotic cells caused chromosome separation defects and increased the G2/M arrest in cells, implying that a decrease in USP24 during mitosis is beneficial to the genomic integrity during mitosis (Fig. 4C)."
USP24 affects cancer malignancy
| 1
USP24 activates cancer malignancy. 1 / 1
| 1

eidos
"Studies have also demonstrated that USP24 promotes cancer malignancy by inducing IL-6 transcription into tumor-infiltrating leukocytes , vascular endothelial cells , and cancer-associated fibroblasts [ 142 ] ."

reach
"Moreover, USP24 negatively regulates the parkin-dependent mitophagy by controlling the expression level of parkin ( Thayer et al., 2020 )."
USP24 affects ZEB1
1 |
1 |

No evidence text available
USP24 affects YWHAG
1 |
1 |

No evidence text available
USP24 affects WWTR1
1 |
1 |

No evidence text available
USP24 affects WWOX
1 |
1 |

No evidence text available
USP24 affects VEGF
| 1
USP24 increases the amount of VEGF. 1 / 1
| 1

reach
"The data indicated that the knockdown of USP24 in M2 macrophages decreased Angiopoirtin-2, CD40L, IL-6, tumor necrosis factor alpha (TNF-alpha), Thrombospondin-1 and vascular endothelial growth factor (VEGF) levels, indicating that USP24 is involved in angiogenesis."
USP24 affects USP24-i-101
| 1
USP24 inhibits USP24-i-101. 1 / 1
| 1

reach
"The USP24-specific inhibitor, USP24-i-101 alone or in combination with immunotherapy anti-CTLA4, profoundly reactivates T cell functions in the TME and markedly induces tumor inhibition."
USP24 affects USP24-7656C
| 1
USP24 activates USP24-7656C. 1 / 1
| 1

reach
"USP24-930T and USP24-7656C increase USP24 expression levels by increasing RNA stability."
USP24 affects US7
1 |
1 |

No evidence text available
USP24 affects ULK1 ubiquitination
| 1
USP24 inhibits ULK1 ubiquitination. 1 / 1
| 1

eidos
"Downregulation of USP24 also enhances ULK1 ubiquitination , thereby increasing protein stability and kinase activity of ULK1 [ 230 ] ."
| PMC
USP24 affects UL9
1 |
1 |

No evidence text available
USP24 affects UL29
1 |
1 |

No evidence text available
USP24 affects UL135
1 |
1 |

No evidence text available
USP24 affects TRIM67
1 |
1 |

No evidence text available
USP24 affects TRIM25
1 |
1 |

No evidence text available
USP24 affects TME.USP24 mRNA
| 1
USP24 increases the amount of TME.USP24 mRNA. 1 / 1
| 1

reach
"Together, IL-6 transcriptionally up-regulates PD-1 expression and stability mediated by USP24 in T cells within the TME.USP24 mRNA expression was up-regulated upon IL-6 treatment (Fig. 4G)."
USP24 affects TEAD4
| 1
USP24 inhibits TEAD4. 1 / 1
| 1

reach
"In our study, our candidate USPs (CNV ≥ 2%) in the HCC were not including USP10, and the screening identified that the downregulation of USP24 expression significantly inhibited the transcriptional activity of YAP1/TEAD4."
USP24 affects TAGLN
1 |
1 |

No evidence text available
USP24 affects SeV-elicited TBK1
| 1
USP24 inhibits SeV-elicited TBK1. 1 / 1
| 1

eidos
"Furthermore , USP2-4 repressed SeV-elicited TBK1 activation [ 155 ] , suggesting that TBK1 is a direct target of USP2-4 ."
USP24 affects SYMPK
1 |
1 |

No evidence text available
USP24 affects STIP1
1 |
1 |

No evidence text available
USP24 affects SPSB4
1 |
1 |

No evidence text available
USP24 affects SOX2
1 |
1 |

No evidence text available
USP24 affects SMAD4
1 |
1 |

No evidence text available
USP24 affects SLC35G1
1 |
1 |

No evidence text available
USP24 affects SLC33A1
1 |
1 |

No evidence text available
USP24 affects SLC30A7
1 |
1 |

No evidence text available
USP24 affects SLC30A4
1 |
1 |

No evidence text available
USP24 affects RUFY1
1 |
1 |

No evidence text available
USP24 affects RPA2
1 |
1 |

No evidence text available
USP24 affects RPA1
1 |
1 |

No evidence text available
USP24 affects RETREG3
1 |
1 |

No evidence text available
USP24 affects RETREG1
1 |
1 |

No evidence text available
USP24 affects RELA
1 |
1 |

No evidence text available
USP24 affects RCOR1
1 |
1 |

No evidence text available
USP24 affects RB1
| 1
| 1

sparser
"Interestingly, the RB1 positive, mutant RB1 tumor was annotated as an RB1-USP24 fusion, whereas the four RB1 negative, mutant RB1 tumors contained two nonsense mutations, a splice site mutation, and exon loss."
USP24 affects PtdIns3P
| 1
USP24 inhibits PtdIns3P. 1 / 1
| 1

reach
"These data suggest that USP24 knockdown stimulates PtdIns3P production."
USP24 affects PRKN
| 1
USP24 inhibits PRKN. 1 / 1
| 1

reach
"Moreover, USP24 negatively regulates the parkin-dependent mitophagy by controlling the expression level of parkin ( Thayer et al., 2020 )."
USP24 affects PRDX1
1 |
1 |

No evidence text available
USP24 affects PPIF
1 |
1 |

No evidence text available
USP24 affects PIH1D1
1 |
1 |

No evidence text available
USP24 affects PIBF1
1 |
1 |

No evidence text available
USP24 affects Oxnad1
1 |
1 |

No evidence text available
USP24 affects NR3C1
1 |
1 |

No evidence text available
USP24 affects NPTN
1 |
1 |

No evidence text available
USP24 affects NBR1
1 |
1 |

No evidence text available
USP24 affects MYC
| 1
USP24 activates MYC. 1 / 1
| 1

reach
"Moreover, a cycloheximide (CHX) chase experiment indicated that the co-expression of Flag-USP24 could significantly prolong the half-life of exogenous Myc-tagged YAP1 compared to the negative control (Fig. 1G)."
USP24 affects MTHFR
| 1
| 1

sparser
"Only three of these genes, USP24 , MTHFR , and EPS15 , have been associated with PD in earlier studies ( xref xref xref )."
USP24 affects MDM2 well-known E3 ligase P53 lung cancer
| 1
USP24 activates MDM2 well-known E3 ligase P53 lung cancer. 1 / 1
| 1

eidos
"Intriguingly , USP24 up-regulates the expression of MDM2 , the well-known E3 ligase of P53 , in lung cancer , which contradicts the above finding 31 ."
USP24 affects LMBR1L
1 |
1 |

No evidence text available
USP24 affects LC3-II
| 1
USP24 activates LC3-II. 1 / 1
| 1

reach
"Herein we found a positive correlation between targeting USP24-mediated LC3-II signaling and the cytotoxicity of Taxol in drug resistance, implying that targeting USP24-induced autophagy might be important for inhibiting drug resistance during cancer therapy (Fig. 1C)."
USP24 affects KRAS
| 1
USP24 increases the amount of KRAS. 1 / 1
| 1

reach
"However, WP1130, the inhibitor of USP9X and USP24, could not reduce the KRAS protein level in MM cells, indicating that USP9X and USP24 are not the DUBs for KRAS (Fig. S7C)."
USP24 affects KDM6B
1 |
1 |

No evidence text available
USP24 affects KDM1A
1 |
1 |

No evidence text available
USP24 affects ISGs
| 1
USP24 increases the amount of ISGs. 1 / 1
| 1

reach
"We showed that USP24 inhibited IFN-I production by targeting TBK1 and reduced the expression of ISGs, thereby promoting EV71 virus infection."
USP24 affects ISG [ 61 ]
| 1
USP24 increases the amount of ISG [ 61 ]. 1 / 1
| 1

reach
"By targeting TBK1, USP24 promotes EV71 infection by inhibiting the production of IFN-I and decreasing the expression of ISG [ 61 ]."
USP24 affects IL17A
| 1
USP24 decreases the amount of IL17A. 1 / 1
| 1

reach
"USP24 positively regulates IL-6, IL-8, IL-22, and IL-24 expression but negatively regulates IL-10 and IL-17 expression."
USP24 affects HTT
1 |
1 |

No evidence text available
USP24 affects HNRNPD
1 |
1 |

No evidence text available
USP24 affects HMGA1
1 |
1 |

No evidence text available
USP24 affects HDAC1
1 |
1 |

No evidence text available
USP24 affects HCST
1 |
1 |

No evidence text available
USP24 affects HCC
| 1
USP24 inhibits HCC. 1 / 1
| 1

sparser
"Previous studies have demonstrated that USP24 inhibited proliferation and migration of HCC, and our findings also indicated that USP24 increased cell death in HCC cells (Supplementary Fig.  xref )."
USP24 affects H2AP
1 |
1 |

No evidence text available
USP24 affects GYPA
1 |
1 |

No evidence text available
USP24 affects GSDMB protein
| 1
USP24 activates GSDMB protein. 1 / 1
| 1

eidos
"USP24 stabilizes GSDMB to promote STAT3 phosphorylation in bladder cancer cells Given that USP24 promoted the expression of the GSDMB protein levels rather than that of the mRNA levels in bladder cancer cells , we hypothesized that USP24 may regulate the stability of GSDMB through the ubiquitinated proteasome pathway ."
USP24 affects GPRC5D
1 |
1 |

No evidence text available
USP24 affects GPR17
1 |
1 |

No evidence text available
USP24 affects GPI
1 |
1 |

No evidence text available
USP24 affects FPR1
1 |
1 |

No evidence text available
USP24 affects FOXK1
1 |
1 |

No evidence text available
USP24 affects FAM168A
1 |
1 |

No evidence text available
USP24 affects EZR
| 1
USP24 activates EZR. 1 / 1
| 1

reach
"Herein, we found that USP24 not only repressed DNA-damage repair (DDR) activity by decreasing Rad51 expression to cause the tumor genomic instability and cancer stemness, but also increased the levels of the ATP binding cassette (ABC) transporters P-gp, ABCG2, and ezrin to enhance the pumping out of Taxol from cancer cells, thus resulted in drug resistance during cancer therapy."
USP24 affects EPS15
| 1
| 1

sparser
"Only three of these genes, USP24 , MTHFR , and EPS15 , have been associated with PD in earlier studies ( xref xref xref )."
USP24 affects EGLN3
1 |
1 |

No evidence text available
USP24 affects EGFR
| 1
USP24 activates EGFR-L858R. 1 / 1
| 1

reach
"USP24 functional knockout inhibits drug resistance in drug resistant mice with gefitinib-induced EGFR L858R lung cancer."
USP24 affects EFTUD2
1 |
1 |

No evidence text available
USP24 affects DHX34
1 |
1 |

No evidence text available
USP24 affects DDR
| 1
USP24 inhibits DDR. 1 / 1
| 1

reach
"Herein, we found that USP24 not only repressed DNA-damage repair (DDR) activity by decreasing Rad51 expression to cause the tumor genomic instability and cancer stemness, but also increased the levels of the ATP binding cassette (ABC) transporters P-gp, ABCG2, and ezrin to enhance the pumping out of Taxol from cancer cells, thus resulted in drug resistance during cancer therapy."
USP24 affects Cyclin
| 1
USP24 increases the amount of Cyclin. 1 / 1
| 1

reach
"Knockdown of USP24 increased the CDK1 and cyclin B1 levels, and increased the ratio of cells in the G2/M stage, indicating that USP24 reduction was beneficial for cell cycle progression (XREF_SUPPLEMENTARY)."
| 1
| 1

reach
"Indeed, USP9X/USP24 promotes cell survival by removing K48-linked polyubiquitin chains from Mcl1, which normally mark the protein for proteasomal degradation [38,39] ."
USP24 affects Cdc6
1 |
1 |

No evidence text available
USP24 affects CYB5B
1 |
1 |

No evidence text available
USP24 affects CUL3
1 |
1 |

No evidence text available
USP24 affects CSNK2A1
1 |
1 |

No evidence text available

reach
"USP24 was reactivated using the deactivated CRISPR associated protein 9 (dCas9)-synergistic activation mediator (SAM) system."
USP24 affects COPS5
1 |
1 |

No evidence text available
USP24 affects CMTM5
1 |
1 |

No evidence text available
USP24 affects CK2
| 1
| 1

reach
"To examine endogenous interaction between RUNX2 and CK2 or USP24, cell lysates were obtained from mouse COBs and prepared in lysis buffer (50mM Tris-HCl (pH 7.4), 150mM NaCl, 1% Triton X-100, 1mM EDTA, 1mM EGTA, 50mM NaF, 1mM Na 3 VO 4, 1mM PMSF, and protease inhibitor cocktail (Sigma))."
USP24 affects CDC5L
1 |
1 |

No evidence text available
USP24 affects CD4
| 1
USP24 activates CD4. 1 / 1
| 1

reach
"Our study demonstrated that USP24 promotes PD-1 protein stability in both CD4 and CD8 T cells and also increases levels on TAMs, suggesting USP24 as a universal regulator in PD-1 expression."
USP24 affects CCNF
1 |
1 |

No evidence text available
USP24 affects CCL2
| 1
USP24 increases the amount of CCL2. 1 / 1
| 1

reach
"However, in this study, USP24 activated NF-kappaB did not increase CCL2, implying that other factors regulated by USP24 might negatively regulate CCL2 expression."
USP24 affects CCAR2
1 |
1 |

No evidence text available
USP24 affects CBL
| 1
USP24 inhibits CBL. 1 / 1
| 1

reach
"S3F), while knockdown of USP24 increased c-Cbl interacting with PD-1 (fig."
USP24 affects CASP3
| 1
USP24 decreases the amount of CASP3. 1 / 1
| 1

reach
"Knockdown of USP24 decreased the Bax and caspase-3 levels; conversely, these protein levels were increased in GFP-USP24-expressing cells, although no alterations were observed in the Bax mRNA level (XREF_FIG; XREF_SUPPLEMENTARY)."
USP24 affects C19orf38
1 |

No evidence text available
USP24 affects BRD2
1 |
1 |

No evidence text available
USP24 affects BH3
| 1
USP24 deubiquitinates BH3. 1 / 1
| 1

reach
"In agreement with previous reports indicating that USP24 may de-ubiquitinate the pro apoptotic BH3 protein BAX [31], we also observed a decrease in BAX protein levels in USP24 knockdown cells)."
USP24 affects B3GAT1
1 |
1 |

No evidence text available
USP24 affects ARRB1
1 |
1 |

No evidence text available
USP24 affects AAR2
1 |
1 |

No evidence text available
USP24 be compensated affects USP24
| 1
USP24 be compensated activates USP24. 1 / 1
| 1

eidos
"Both sets of experiments demonstrated that USP24 expression was abolished and that U2OS cells deleted for USP24 are viable , most likely because loss of USP24 can be compensated for by cellular reprogramming or the presence of other , compensating USPs in the cell ."
USP inhibitor affects USP24
| 1
USP inhibitor inhibits USP24. 1 / 1
| 1

reach
"EOAI3402143(G9) is a non-specific USP inhibitor that inhibits USP5, USP9X and USP24 in a dose-dependent manner."
US7 affects USP24
1 |
1 |

No evidence text available
ULK1 affects USP24
| 1
Ubiquitinated ULK1 leads to the deubiquitination of USP24. 1 / 1
| 1

reach
"Unlike USP20, ULK1 ubiquitination blocks autophagic flux after starvation and is attenuated by two deubiquitinating enzymes, USP1 and USP24."
UL9 affects USP24
1 |
1 |

No evidence text available
UL29 affects USP24
1 |
1 |

No evidence text available
UL135 affects USP24
1 |
1 |

No evidence text available
TRIM67 affects USP24
1 |
1 |

No evidence text available
TRIM25 affects USP24
1 |
1 |

No evidence text available
TAGLN affects USP24
1 |
1 |

No evidence text available
SYMPK affects USP24
1 |
1 |

No evidence text available
STIP1 affects USP24
1 |
1 |

No evidence text available
SPSB4 affects USP24
1 |
1 |

No evidence text available
SOX2 affects USP24
1 |
1 |

No evidence text available
SMARCA4 affects BRD7
| 1
| 1

sparser
"Next, when HA-BRD7 and HA-BRG1 were expressed in H1299 cells or MEFs, we found that USP24 interacted with BRD7 and BRG1, indicating that USP24 could interact with more than one BRD-containing protein in different cell lines (Fig.  xref B,C and Suppl."
SMAD4 affects USP24
1 |
1 |

No evidence text available
SLC35G1 affects USP24
1 |
1 |

No evidence text available
SLC33A1 affects USP24
1 |
1 |

No evidence text available
SLC30A7 affects USP24
1 |
1 |

No evidence text available
SLC30A4 affects USP24
1 |
1 |

No evidence text available
RUFY1 affects USP24
1 |
1 |

No evidence text available
RPA2 affects USP24
1 |
1 |

No evidence text available
RPA1 affects USP24
1 |
1 |

No evidence text available
RETREG3 affects USP24
1 |
1 |

No evidence text available
RETREG1 affects USP24
1 |
1 |

No evidence text available
RELA affects USP24
1 |
1 |

No evidence text available
RCOR1 affects USP24
1 |
1 |

No evidence text available
RB1 affects USP24
| 1
| 1

sparser
"Interestingly, the RB1 positive, mutant RB1 tumor was annotated as an RB1-USP24 fusion, whereas the four RB1 negative, mutant RB1 tumors contained two nonsense mutations, a splice site mutation, and exon loss."
Proteasome affects USP24
| 1

reach
"The ubiquitin hydrolase USP24 directly interacts with p300 protein, and reduces p300 protein ubiquitination and proteasome-mediated degradation, leading to p300 protein up-regulation and histone acetylation 15 (Figure 1C)."
PRDX1 affects USP24
1 |
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No evidence text available
PPIF affects USP24
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No evidence text available
PIH1D1 affects USP24
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No evidence text available
PIBF1 affects USP24
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No evidence text available
PDC affects USP24
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PDC activates USP24. 1 / 1
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"The expression level of USP24 was inversely correlated with miR-139-5p expression in SSc patients and induced by TLR9 stimulation in healthy pDCs ."
Oxnad1 affects USP24
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No evidence text available
Other factor(s) affects USP24
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Other factor(s) activates USP24. 1 / 1
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"Other factor (s) in addition to the EGFR activated pathway may be involved in the regulation of USP24 during tumorigenesis."
Nitrates affects USP24
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Nitrates increases the amount of USP24. 1 / 1
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No evidence text available
Neoplasms affects USP24
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Neoplasms decreases the amount of USP24. 1 / 1
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"We further found that patients whose tumors had reduced expression of USP24, defined as less than the 20 percentile, were significantly more likely to suffer disease progression and to die of any cause."
NR3C1 affects USP24
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No evidence text available
NPTN affects USP24
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No evidence text available
NOTCH1 affects USP9X
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"Indeed, our pull-down studies for NOTCH1, followed by detection of USP24 and USP9x in western blot experiments, showed that neither USP9x nor USP24 interact with NOTCH1 ( xref )."
NFKB1 affects USP24
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"The gene promoter of USP24 contains a Nuclear Factor kappa-light-chain of activated B cells (NFkB) binding site, where increased NFkB levels or activity upon stress or inflammation was shown to increase USP24 promoter activity and gene expression [167]."
NF-kappa B. J Neurochem 2014 affects USP24
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NF-kappa B. J Neurochem 2014 increases the amount of USP24. 1 / 1
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"Wang K, Liu S, Wang J, Wu Y, Cai F, Song W. Transcriptional regulation of human USP24 gene expression by NF-kappa B. J Neurochem 2014; 128:818-28; PMID :24286619; http://dx.doi.org/10.1111/ jnc.12626 8."
NBR1 affects USP24
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MTHFR affects USP24
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"Only three of these genes, USP24 , MTHFR , and EPS15 , have been associated with PD in earlier studies ( xref xref xref )."
MIR935 affects USP24
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MIR935 decreases the amount of USP24. 1 / 1
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No evidence text available
LY294002 affects USP24
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LY294002 leads to the dephosphorylation of USP24. 1 / 1
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"In addition, we found that USP24 phosphorylation at Ser2604 was declined by U0126, which inhibited the Erk1/2 activities in A549 cells (XREF_FIG); however, USP24 phosphorylation was not decreased by LY294002, which inhibited the PI3K activities (XREF_SUPPLEMENTARY)."
LMBR1L affects USP24
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No evidence text available
LC3-II affects USP24
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LC3-II inhibits USP24. 1 / 1
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"Accumulation of LC3-II following knockdown of USP24 was further enhanced by the treatment with lysosomal inhibitor chloroquine)."
KDM6B affects USP24
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No evidence text available
KDM1A affects USP24
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No evidence text available
HTT affects USP24
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No evidence text available
HNRNPD affects USP24
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No evidence text available
HMGA1 affects USP24
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No evidence text available
HDAC1 affects USP24
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No evidence text available
HCST affects USP24
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No evidence text available
H2AP affects USP24
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No evidence text available
GYPA affects USP24
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No evidence text available
GPRC5D affects USP24
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No evidence text available
GPR17 affects USP24
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No evidence text available
GPI affects USP24
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No evidence text available
FTI-276 affects USP24
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FTI-276 increases the amount of USP24. 1 / 1
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"Gefitinib and FTI-276, which inhibited the EGFR and Kras activities 21 in the A431 and A549 cell lines, respectively, increased USP24 expression (XREF_FIG)."
FPR1 affects USP24
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No evidence text available
FOXK1 affects USP24
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No evidence text available
FAM168A affects USP24
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No evidence text available
EPS15 affects USP24
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"Only three of these genes, USP24 , MTHFR , and EPS15 , have been associated with PD in earlier studies ( xref xref xref )."
EGLN3 affects USP24
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No evidence text available
EGFR affects USP24
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Mutated EGFR inhibits USP24. 1 / 1
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"Therefore, during the initiation of tumor formation, EGFR mutations decrease USP24 to increase the degradation of p300 and Bax, and thus repress cell apoptosis."
EFTUD2 affects USP24
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No evidence text available
E2F1 affects USP24
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E2F1 inhibits USP24. 1 / 1
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"Overexpression of E2F4 in USP24 silenced cells rescued only the E2F1 mRNA level and partially abolished the effect of the USP24 knockdown on the G1-S transition and colony formation (XREF_FIG; XREF_SUPPLEMENTARY), implying that E2F4 might be involved in USP24 mediated cell cycle progression."
DHX34 affects USP24
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No evidence text available
Cdc6 affects USP24
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No evidence text available
CYB5B affects USP24
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No evidence text available
CUL3 affects USP24
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No evidence text available
CSNK2A1 affects USP24
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No evidence text available
COPS5 affects USP24
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No evidence text available
CMTM5 affects USP24
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No evidence text available
CK2 affects USP24
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"To examine endogenous interaction between RUNX2 and CK2 or USP24, cell lysates were obtained from mouse COBs and prepared in lysis buffer (50mM Tris-HCl (pH 7.4), 150mM NaCl, 1% Triton X-100, 1mM EDTA, 1mM EGTA, 50mM NaF, 1mM Na 3 VO 4, 1mM PMSF, and protease inhibitor cocktail (Sigma))."
CDH1 affects USP24
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"After knocking down USP24, the level of E-cadherin was upregulated, and the levels of vimentin, fibronectin, and N-cadherin were downregulated in drug-resistant cells, while overexpression of miR-21-5[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
CDC5L affects USP24
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No evidence text available
CD274 affects USP24
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"Interestingly, we found that USP24 can interact with PD-L1 in interphase and mitotic cells, implying that USP24 may stabilize PD-L1 in cancer drug-resistant cells PC9-GR (Fig.  xref ) and A549-T24 cells (Fig.  xref )."
CCNF affects USP24
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No evidence text available
CCAR2 affects USP24
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No evidence text available
C19orf38 affects USP24
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No evidence text available
BRD7 affects SMARCA4, and USP24
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"Next, when HA-BRD7 and HA-BRG1 were expressed in H1299 cells or MEFs, we found that USP24 interacted with BRD7 and BRG1, indicating that USP24 could interact with more than one BRD-containing protein in different cell lines (Fig.  xref B,C and Suppl."
BRD2 affects USP24
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No evidence text available
B3GAT1 affects USP24
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No evidence text available
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Air Pollutants increases the amount of USP24. 1 / 1
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No evidence text available
ARRB1 affects USP24
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No evidence text available
AAR2 affects USP24
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No evidence text available
2,4,6-tribromophenol decreases the amount of USP24. 1 / 1
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No evidence text available
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No evidence text available
1-phenylpropan-2-amine decreases the amount of USP24. 1 / 1
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No evidence text available

No evidence text available

No evidence text available
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No evidence text available