IndraLab

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"To examine endogenous interaction between RUNX2 and CK2 or USP24, cell lysates were obtained from mouse COBs and prepared in lysis buffer (50mM Tris-HCl (pH 7.4), 150mM NaCl, 1% Triton X-100, 1mM EDTA, 1mM EGTA, 50mM NaF, 1mM Na 3 VO 4, 1mM PMSF, and protease inhibitor cocktail (Sigma))."