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MYSM1 affects STING1
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MYSM1 binds STING1.
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STING1 binds MYSM1. 10 / 22
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"In transfected HEK293T cells, MYSM1 interacted with STING, but not with cGAS, TBK1, or IRF3 ( Figure S3 A)."
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"In HSV-1-infected THP-1 differentiated macrophages, endogenous MYSM1 interacted strongly with STING at 2 h post-infection ( Figure 3 E)."
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"The domains required for MYSM1-STING interaction were determined by analyzing mutated forms of STING and MYSM1 ( Figures S4 A and S4B)."
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"MYSM1 interacted with STING and STING(1–190) but not with STING(191–379) ( Figure S4 C)."
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"STING interacted strongly with MYSM1 and weakly with MYSM1(ΔSANT) and MYSM1(ΔMPN), but failed to interact with MYSM1(ΔSWIRM) ( Figure S4 D)."
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"Additionally, the deubiquitinating enzyme MYSM1 interacts with and modifies STING, preventing downstream signaling and offering new therapeutic avenues (69)."
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MYSM1 binds STING1 and K63. 2 / 2
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"Activated MYSM1 binds to STING and removes its K63-linked polyubiquitination, repressing the synthesis of type I IFNs and pro-inflammatory cytokines, including IFN-β, IFN-stimulated gene 56 (ISG56), i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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MYSM1 binds STING1 and MPN domain. 1 / 1
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"We have shown that the SWIRM domain is necessary for MYSM1 interaction with STING, and the MPN domain is necessary for STING deubiquitination.In addition to the defense against viral infections, STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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MYSM1 deubiquitinates STING1.
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MYSM1 deubiquitinates STING1. 4 / 4
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"In addition, UB-K63-mediated polyubiquitination of STING was attenuated by purified MYSM1 ( Figure 3 K)."
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"In HEK293T cells, MYSM1 deubiquitinates STING but not STING(K150R) ( Figure 3 M)."
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"Since MYSM1 deubiquitinates STING, the effect of MYSM1 on the dimerization and aggregation of STING was evaluated."
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"<span class="match term0">MYSM1</span> subsequently interacts with <span class="match term1">STING</span> and cleaves <span class="match term1">STING</span> K63-linked ubiquitination to suppress cGAS-<span class="match term1">STING</span> signaling"
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MYSM1 deubiquitinates STING1-K150R. 1 / 1
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"In HEK293T cells, MYSM1 deubiquitinates STING but not STING(K150R) ( Figure 3 M)."
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MYSM1 inhibits STING1.
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MYSM1 inhibits STING1. 2 / 2
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"Moreover, cGAMP induced the upregulation of IFN-β , IL-6 , and Cxcl10 mRNAs ( Figure 3 C), as well as IFN-β, IL-6, and TNF-α proteins ( Figure 3 D), and was significantly higher in BMDMs from Mysm1 −/[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In addition, STING aggregation and STING-TBK1 interaction were significantly promoted in Mysm1 −/− mice, indicating that MYSM1 restricts STING activation by interacting with STING."
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MYSM1 bound to K63 and STING1 inhibits STING1. 1 / 1
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"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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MYSM1 affects STAT1
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MYSM1 binds STAT1.
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STAT1 binds MYSM1. 10 / 15
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"Besides, we found that oeMYSM1 only promoted the mRNA expression of necroptosis-related genes ( Mlkl / Ripk3 ) rather than other PCD-related genes ( Ripk1 / Fas / Zbp1 / Casp3 ) in H/R-induced HL-1 cells ( xref B-G), indicating that MYSM1-STAT1 axis up-regulated the transcription and expression of necroptosis-related genes."
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"These findings confirmed a direct interaction between MYSM1 and STAT1, suggesting that STAT1 may be a crucial substrate protein of MYSM1."
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"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain."
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"In conclusion, this study illustrated a MYSM1-STAT1 axis in regulating myocardial I/R injury and identified MYSM1 as a pharmacological target for myocardial I/R injury."
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"Therefore, we speculated that MYSM1 may utilize STAT1 as a crucial substrate in the regulation of myocardial I/R injury.Then we examined the direct interaction between MYSM1 and STAT1."
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"These findings confirmed a direct interaction between MYSM1 and STAT1, suggesting that STAT1 may be a crucial substrate protein of MYSM1."
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"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
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"In this study, we demonstrated a specific MYSM1-STAT1 axis in myocardial I/R injury, which might avoid the side effects of targeting STAT1 alone, indicating that targeting MYSM1 could be a more specific therapeutic strategy for myocardial I/R injury."
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"Apart from the MYSM1-STAT1 interaction, MYSM1 has been reported to deubiquitinate other substrate proteins xref , such as E2A xref , GATA2 xref , and histone H2A xref ."
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"Then we examined the direct interaction between MYSM1 and STAT1."
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MYSM1 binds STAT1 and SWIRM domain. 2 / 2
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"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
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"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
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MYSM1 binds STAT1 and MPN domain.According. 1 / 1
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"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain.According to our ubiquitinomics, the K379 residue of STAT1, located in the DNA binding domain (DBD) of STAT1 (Figure 6K), was the most significantly reduced deubiquitination residue of STAT1 after MYSM1 overexpression (Figure 5E)."
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MYSM1 deubiquitinates STAT1.
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MYSM1 deubiquitinates STAT1. 5 / 5
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"MYSM1 deubiquitinates STAT1 and maintains the stability of STAT1."
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"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain.According to our ubiquitinomics, the K379 residue of STAT1, located in the DNA binding domain (DBD) of STAT1 (Figure 6K), was the most significantly reduced deubiquitination residue of STAT1 after MYSM1 overexpression (Figure 5E)."
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"In this study, we demonstrated that MYSM1 deubiquitinates and stabilizes STAT1 through the MPN metalloprotease domain of MYSM1."
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"MYSM1 promotes the Mlkl and Ripk3 expression by deubiquitinating and stabilizing STAT1."
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"In addition to STAT1, we also screen out some potential substrates from our comprehensive proteome-wide quantitative analysis, including Actn4, Aldoa, Isg15, S100a4, Cct7 and Lgals8, which is worthy of further exploration.In conclusion, we showed for the first time that MYSM1 deubiquitinates and stabilizes STAT1 to increase the transcription of necroptosis-related genes in cardiomyocyte under I/R injury."
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MYSM1 activates STAT1.
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MYSM1 activates STAT1. 5 / 5
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"MYSM1 was found to enhance the protein stability of STAT1, which correspondingly resulted in an increased phosphorylation level of STAT1."
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"Taken together, these results suggested that MYSM1 promotes STAT1 stability by cleaving the polyubiquitin chains on K379 residue of STAT1 (Figure 6M)."
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"In summary, these results showed that MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1."
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"Mechanistically, MYSM1 promotes the stability of STAT1 by cleaving polyubiquitin chains from the K379 residue of STAT1."
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"In addition, MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1."
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MYSM1 phosphorylates STAT1.
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MYSM1 leads to the phosphorylation of STAT1. 2 / 2
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"These results indicate that MYSM1 primarily enhances STAT1 phosphorylation by increasing the stability of the STAT1 protein, rather than through its receptor tyrosine kinases or phosphatases.We then explored the regulation of MYSM1 on STAT1 ubiquitination."
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"These results indicate that MYSM1 primarily enhances STAT1 phosphorylation by increasing the stability of the STAT1 protein, rather than through its receptor tyrosine kinases or phosphatases."
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MYSM1 leads to the phosphorylation of STAT1 on tyrosine. 1 / 1
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"However, it is worth exploring whether MYSM1 modulates STAT1 phosphorylation via receptor tyrosine kinases or phosphatases."
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TP53 affects MYSM1
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TP53 activates MYSM1.
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TP53 activates MYSM1. 10 / 13
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"Our study also provides important insights into the unique effects of p53 stress responses in HSCs, where p53 activation in Mysm1 deficiency does not result in apoptosis, but promotes loss of quiescence."
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"As induction of Cdkn2a and p19 ARF was previously implicated in p53 activation in Mysm1 -/- thymocytes, 39 we conclude that the mechanisms of p53 activation in Mysm1 deficient HSPCs are distinct and p19 ARF -independent."
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"36 Belle JI, Langlais D, Petrov JC, Pardo M, Jones RG, Gros P, et al. p53 mediates loss of hematopoietic stem cell function and lymphopenia in Mysm1 deficiency."
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"In summary, our results demonstrate novel roles for Mysm1 in osteoblast differentiation and osteoclast formation, resulting in osteopenia in Mysm1 deficient mice that could be abrogated by the loss of p53 from increased osteogenic differentiation of Mysm1 -/- p53 -/- MSCs.-Haffner-Luntzer, M., Kovtun, A., Fischer, V., Prystaz, K., Hainzl, A., Kroeger, C. M., Krikki, I., Brinker, T. J., Ignatius, A., Gatzka, M. Loss of p53 compensates osteopenia in murine Mysm1 deficiency."
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"To our knowledge, the role of p53 activation in Mysm1 deficient NK cell dysfunction has not been addressed [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
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"The suggested triggers for p53 activation in Mysm1 -/- include the putative roles of MYSM1 in the maintenance of p19ARF expression within the thymus [XREF_BIBR], and IRF2 and IRF8 expression in bone marrow progenitor cells [XREF_BIBR]."
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"Deletion of p53 rescues B-cell populations in MYSM1-deficient mice."
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"As increased p19 ARF / p53 activation in developing Mysm1 deficient mice could be a consequence of checkpoint activation, a default pathway, or be induced as a result of a lack of 2A-DUB/Mysm1 action in regulatory complexes involved in repression of the Cdkn2a (Ink4 and Arf) locus, we next set out to explore Mysm1 binding to fragments of the p19 ARF promoter in activated thymocytes."
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"However, the mechanisms triggering p53 activation in Mysm1 -/- HSPCs, and the pathways downstream of p53 driving different aspects of the Mysm1 -/- phenotype remain unknown."
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"Belle et al. 34 and Gatzka et al. 35 independently generated Mysm1 -/- p53 -/- mice that largely restored Mysm1 deficiency associated defects."
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Modified TP53 activates MYSM1. 1 / 1
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"36 Here, we observed enhanced p53 levels in Poly (I : C)-treated mice that paralleled increases in Mysm1 expression, and that there was enhanced p53 transcription in Mysm1 -/- HSCs."
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TP53 binds MYSM1.
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MYSM1 binds TP53. 10 / 11
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"At the Cdkn1a/ p21 locus, increased H3K27ac and H3K4me3 levels were also observed, but without increased p53 binding ( xref ), which indicates that MYSM1 can have an impact on H3K27ac levels independently of effects on p53 recruitment."
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"Interaction between MYSM1 and p53 proteins was demonstrated in hematopoietic cell lines through co-immunoprecipitation (co-IP) [ xref ], and MYSM1 binding to the promoters of several p53 stress response genes was also reported, suggesting possible mechanisms for p53 regulation by MYSM1."
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"Co‐immunoprecipitation (CO‐IP) results indicated that Mysm1 interacts with p53 (Figure  xref )."
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"We thus generated double-knockout mice to assess a potential genetic interaction between Mysm1 and p53 in hematopoiesis."
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"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 and PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a and p21."
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"Given the recruitment of MYSM1 to genomic p53 binding sites, we tested for interaction between MYSM1 and p53 proteins."
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"MYSM1 therefore interacts with p53, either directly or more likely as part of a larger protein complex."
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"We thus generated double-knockout mice to assess a potential genetic interaction between Mysm1 and p53 in hematopoiesis."
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"Given the recruitment of MYSM1 to genomic p53-binding sites, we tested for interaction between MYSM1 and p53 proteins."
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"MYSM1 therefore interacts with p53, either directly or more likely as part of a larger protein complex."
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TP53 binds ARF and MYSM1. 1 / 1
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"In line with a previous report on Mysm1-binding to thep19 ARF -promoter in thymocytes [ xref ], an upstream regulator of TS p53, in Mysm1 −/− epidermal cells as well, mRNA expression ofp19 ARF was moderately increased along with increases in p53 levels and in apoptosis."
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MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
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"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
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TP53 inhibits MYSM1.
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TP53 inhibits MYSM1. 1 / 1
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"While several mechanisms for p53 activation in MYSM1 deficiency were proposed, no clear link to MYSM1 DUB catalytic activity has emerged so far ."
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MYSM1 affects TP53
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MYSM1 binds TP53.
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MYSM1 binds TP53. 10 / 11
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"At the Cdkn1a/ p21 locus, increased H3K27ac and H3K4me3 levels were also observed, but without increased p53 binding ( xref ), which indicates that MYSM1 can have an impact on H3K27ac levels independently of effects on p53 recruitment."
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"Interaction between MYSM1 and p53 proteins was demonstrated in hematopoietic cell lines through co-immunoprecipitation (co-IP) [ xref ], and MYSM1 binding to the promoters of several p53 stress response genes was also reported, suggesting possible mechanisms for p53 regulation by MYSM1."
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"Co‐immunoprecipitation (CO‐IP) results indicated that Mysm1 interacts with p53 (Figure  xref )."
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"We thus generated double-knockout mice to assess a potential genetic interaction between Mysm1 and p53 in hematopoiesis."
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"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 and PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a and p21."
26768662
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"Given the recruitment of MYSM1 to genomic p53 binding sites, we tested for interaction between MYSM1 and p53 proteins."
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"MYSM1 therefore interacts with p53, either directly or more likely as part of a larger protein complex."
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"We thus generated double-knockout mice to assess a potential genetic interaction between Mysm1 and p53 in hematopoiesis."
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"Given the recruitment of MYSM1 to genomic p53-binding sites, we tested for interaction between MYSM1 and p53 proteins."
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"MYSM1 therefore interacts with p53, either directly or more likely as part of a larger protein complex."
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TP53 binds ARF and MYSM1. 1 / 1
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"In line with a previous report on Mysm1-binding to thep19 ARF -promoter in thymocytes [ xref ], an upstream regulator of TS p53, in Mysm1 −/− epidermal cells as well, mRNA expression ofp19 ARF was moderately increased along with increases in p53 levels and in apoptosis."
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MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
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"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
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MYSM1 inhibits TP53.
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MYSM1 inhibits TP53. 7 / 7
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"These results suggested that the decrease of Mysm1 reduces the threshold of calcium mobilization and makes astrocytes easier to release more ATP.2.5 Mysm1 Knockdown Activated the p53/AMPK Pathway."
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"As previously reported, Mysm1 deficiency could promote p53 pathway activation, thereafter, p53 could activate AMPK, which is involved in regulating the balance of energy metabolism."
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"22 , 23 We set out to verify whether Mysm1 knockdown can activate the p53/AMPK pathway in primary astrocytes and C8‐D1A cells and then to evaluate the ATP level."
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"53 Our work shows that MYSM1 represses p53 stress response genes by antagonizing other histone modifications (H3K27ac and H3K4me3)."
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"As Mysm1 generally functions as a transcription activator, it is rational to postulate that Mysm1 suppresses p53 or p19 ARF likely by activating expression of transcription repressors."
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"Mysm1-deficiency is known to result in p53 activation in hematopoietic cells [XREF_BIBR, XREF_BIBR, XREF_BIBR], indicating that MYSM1 normally represses p53 activation, and suggesting it as a possible target for therapeutic p53 activation in hematological malignancies that retain wild type p53 function."
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"Mysm1-deficiency is known to result in p53-activation in hematopoietic cells [ 19,44,45 ] , indicating that MYSM1 normally represses p53 activation , and suggesting it as a possible target for therapeutic p53-activation in hematological malignancies that retain wild type p53 function ."
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MYSM1 increases the amount of TP53.
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MYSM1 increases the amount of TP53. 3 / 3
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"Nijnik et al. 15 demonstrated that Mysm1 -/- mice have enhanced levels of p53, which is a central regulator of cellular stress responses."
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"To investigate the underlying mechanisms, we first confirmed that Mysm1 -/- mice had enhanced p53 levels in the Lin - HSCs and LSKs as determined by ICS assays (XREF_FIG)."
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"Further studies revealed that Mysm1 suppressed p53-target gene Bbc3 and PUMA for HSC maintenance 49 and Mysm1 -/- enhanced p53 levels by upregulation of p19 ARF."
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MYSM1 activates TP53.
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MYSM1 activates TP53. 3 / 3
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"We recently demonstrated that all developmental and hematopoietic phenotypes of Mysm1 deficiency are p53 mediated and rescued in the Mysm1 -/- p53 -/- mouse model."
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"Furthermore, we identify potential mediators of p53 dependent but PUMA independent Mysm1 -/- hematopoietic deficiency phenotypes."
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"Overall, we demonstrate that the loss of MYSM1 in mouse B cell lymphoma represses the induction of ribosomal protein genes, reduces cellular protein synthesis rate, promotes p53 activation and potently inhibits cMYC oncogenic functions.To compare the location of the genomic binding sites of MYSM1 and cMYC, we consolidated the ChIP‐Seq datasets for cMYC and its dimerization partner MAX from multipotent haematopoietic progenitor cells HPC7 12 , 13 with the MYSM1 ChIP‐Seq acquired in our recent work in a B cell progenitor cell line Ba/F3."
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MYSM1 decreases the amount of TP53.
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MYSM1 decreases the amount of TP53. 2 / 2
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"IRF2 and IRF8 reduction in Mysm1 -/- HSCs enhances p53 levels."
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"Our work demonstrates that MYSM1 represses p53 stress response gene expression by localizing to p53 binding sites within Bbc3 and PUMA and Cdkn1a and p21 promoters and antagonizing local histone modification (H3K27ac and H3K4me3) and p53 recruitment (XREF_FIG)."
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STING1 affects MYSM1
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STING1 binds MYSM1. 10 / 22
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"In transfected HEK293T cells, MYSM1 interacted with STING, but not with cGAS, TBK1, or IRF3 ( Figure S3 A)."
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"In HSV-1-infected THP-1 differentiated macrophages, endogenous MYSM1 interacted strongly with STING at 2 h post-infection ( Figure 3 E)."
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"The domains required for MYSM1-STING interaction were determined by analyzing mutated forms of STING and MYSM1 ( Figures S4 A and S4B)."
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"MYSM1 interacted with STING and STING(1–190) but not with STING(191–379) ( Figure S4 C)."
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"STING interacted strongly with MYSM1 and weakly with MYSM1(ΔSANT) and MYSM1(ΔMPN), but failed to interact with MYSM1(ΔSWIRM) ( Figure S4 D)."
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"Additionally, the deubiquitinating enzyme MYSM1 interacts with and modifies STING, preventing downstream signaling and offering new therapeutic avenues (69)."
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MYSM1 binds STING1 and K63. 2 / 2
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"Activated MYSM1 binds to STING and removes its K63-linked polyubiquitination, repressing the synthesis of type I IFNs and pro-inflammatory cytokines, including IFN-β, IFN-stimulated gene 56 (ISG56), i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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MYSM1 binds STING1 and MPN domain. 1 / 1
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"We have shown that the SWIRM domain is necessary for MYSM1 interaction with STING, and the MPN domain is necessary for STING deubiquitination.In addition to the defense against viral infections, STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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MYSM1 affects TRIM21
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MYSM1 binds TRIM21.
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MYSM1 binds TRIM21. 10 / 13
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"Consistently, our data indicated that MYSM1 binds to TRIM21 in a SWIRM-dependent manner."
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"As depicted in Fig.  xref B, it was revealed that the SWIRM domain, but not the SANT or MPN domains, was involved in the interaction between MYSM1 and TRIM21."
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"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig.  xref F)."
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"Through a Co-IP combined LC-MS/MS analysis, we found that MYSM1 directly interacted with tripartite motif-containing protein 21 (TRIM21)."
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"Consistently, our data indicated that MYSM1 binds to TRIM21 in a SWIRM-dependent manner."
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"MYSM1 interacts with TRIM21 to maintain the stability of TRIM21 protein."
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"We conducted preliminary validation of the LC-MS/MS results and found that the interaction between endogenous MYSM1 and TRIM21 was observed in DOX-treated cardiac tissues (Fig.  xref E) and HL-1 cells (Fig.  xref F)."
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"These findings confirmed the existence of a direct interaction between MYSM1 and TRIM21."
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"We conducted preliminary validation of the LC-MS/MS results and found that the interaction between endogenous MYSM1 and TRIM21 was observed in DOX-treated cardiac tissues (Fig. 5E) and HL-1 cells (Fig. 5F)."
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"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
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MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
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"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
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"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
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MYSM1 binds TRIM21 and MPN domain. 2 / 2
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"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
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"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
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MYSM1 deubiquitinates TRIM21.
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MYSM1 deubiquitinates TRIM21. 5 / 5
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"Consistently, MYSM1 reduced the K63-linked ubiquitination of TRIM21 but not K48-linkage."
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"These findings supported the notion that MYSM1 deubiquitinates and regulates the stability of TRIM21 protein."
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"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
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"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
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"Our study revealed that MYSM1 deubiquitinates and stabilizes TRIM21 through its MPN metalloprotease domain.Another important conclusion of this study was that TRIM21 is the potential substrate of MYSM1 in DOX injury."
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MYSM1 increases the amount of TRIM21.
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MYSM1 increases the amount of TRIM21. 1 / 1
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"We transfected NIH/3T3 cells with oeMYSM1 and observed an increase in TRIM21 protein levels following overexpression of MYSM1 (Fig. 5I)."
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MYSM1 affects Flag
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Flag binds MYSM1. 10 / 21
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"To determine the domain required for MYSM1-STAT1 interaction, we co-transfected NIH/3T3 cells with Myc-STAT1, Flag-MYSM1 (MYSM1-WT) or its mutants lacking SANT domain (△SANT), SWIRM domain (△SWIRM), or MPN domain (△MPN) (Figure xref F)."
39897566
sparser
"In differential display analyses, relevant DNA damage-dependent MYSM1 candidate interaction partners were defined as proteins differentially enriched by at least 4-fold (log2(4) = 2) in MYSM1-Flag eluates upon etoposide vs. DMSO treatment by anti-Flag vs. IgG IP, but not present in GFP-Flag eluates ( xref A)."
32466590
sparser
"CUT&Tag using antibodies against Flag and analysis with deepTools revealed a gradual enhanced enrichment of Flag peaks in NSCs that stably expressed Mysm1-Flag with increasing induction time (Fig. xref )."
38342917
sparser
"Exogenous co-immunoprecipitation in HEK-293T cells co-transfected with Flag–MYSM1 and HA–TRAF2 or HA–TRAF3 similarly validated these interactions (Fig. xref )."
41162619
sparser
"Analogous to the loss-of-function assessment of MYSM1, the gain-of-function of MYSM1 was evaluated by transfecting HL-1 cells with Flag-MYSM1 plasmid (oeMYSM1, Fig. xref D)."
39695708
sparser
"A total of 256 modified proteins showed significantly reduced ubiquitination levels in cells overexpressing Flag-MYSM1 (Figure xref A below)."
39897566
sparser
"The results demonstrated that exogenous Flag-MYSM1 and Myc-STAT1 were also efficiently co-immunoprecipitated (Figure xref H)."
39897566
sparser
"In addition, as proof of principle of the co-IP approach, MYSM1 interaction with ubiquitinated histone H2A (H2Aub), a known substrate of the H2A deubiquitinase, was detectable in the anti-Flag pull-down from MYSM1-Flag transfectants, as expected ( xref C, lower panel)."
32466590
sparser
"As presented inFigure xref C-D, the degradation rate of STAT1 was reduced in Flag-MYSM1 overexpressing NIH/3T3 cells under CHX treatment."
39897566
sparser
"NIH/3T3 cells were co-transfected with Myc-TRIM21, Flag-MYSM1 (MYSM1-WT) or its mutants lacking SANT domain (△SANT, aa 113–164), SWIRM domain (△SWIRM, aa 363–464), or MPN domain (△MPN aa 563–673) (Fig.  xref A)."
39695708
MYSM1 activates cell population proliferation. 10 / 12
| 1 11
reach
"Survival and proliferation of melanoma cells is supported by MYSM1."
28978033
reach
"Mysm1 -/- mice had enhanced HSC proliferation that led to a large dysfunctional LSK population."
27277682
reach
"On the other hand, our results also demonstrated that MYSM1 accelerates cell proliferation even in the absence of E2, suggesting that MYSM1 may also participate in regulating non-canonic ERα signaling pathway independent on E2 treatment to participate in promoting breast cancer process (Fig. 5A,B)."
38177530
eidos
"These findings indicated that MYSM1 promotes cell proliferation and colony formation and attenuates SA-beta-Gal activation to repress senescence ."
33240758
reach
"In a separate study, Mysm1 -/- macrophages were confirmed to have elevated production of pro inflammatory cytokines in response to stimulation, as well as increased proliferation, increased apoptosis, and enhanced capacity to control melanoma tumor growth in vivo in mouse models [XREF_BIBR]."
32344625
reach
"As a deubiquitinase, MYSM1 stabilizes ERα protein through its MPN enzyme catalytic domain and is recruited with ERα at the promoters of E2-induced genes, thereby triggering transcription initiation and subsequent acceleration in cell proliferation and suppression of antiestrogen sensitivity."
38177530
reach
"Knockdown of MYSM1 promoted cell proliferation and suppressed senescence of CRPC cells under condition of androgen ablation."
31761786
reach
"Consistently, growth curve analysis showed that knockdown of MYSM1 inhibited cell proliferation, and ectopic expression of ERα partly rescued this condition (Figs."
38177530
reach
"Above all, the results indicate that MYSM1 promotes cell proliferation and G1-S phase transition in BCa, and the effect of MYSM1 on cell growth is partially related to ERα pathway.To test the functional of MYSM1 on BCa cell growth in vivo, MCF-7 cells infected with shMYSM1 or negative control lentivirus (shCtrl) were individually implanted subcutaneously into flank sides of 4-week-old female BALB/c nude mice (n = 7) armpit."
38177530
reach
"Meanwhile, MYSM1 knockout reduces the proliferation of osteoclast progenitor and the osteoclast resorption activity [130]."
30123285
MYSM1 inhibits cell population proliferation. 8 / 8
| 7 1
reach
"Mysm1 knockdown in NSCs leads to NSC proliferation and early differentiation into neurons."
38342917
reach
"It was found that MYSM-1 could suppress the proliferation, migration, and invasion of renal carcinoma cells."
26409454
reach
"Mysm1 deletion promoted NSC proliferation and apoptosis, resulting in depletion of the stem cell pool."
38342917
reach
"Additionally, knockdown Mysm1 in aged mice could promote NSC proliferation."
38342917
reach
"Mysm1 knockdown promotes the proliferation and increases susceptibility to apoptosis of NSCs in vitro."
38342917
reach
"Mysm1 knockdown promotes NSC proliferation in the brains of aged mice."
38342917
reach
"These results revealed that Mysm1 knockdown in NSCs of aged mice promoted NSC proliferation in vivo."
38342917
sparser
"In conclusion, we reveal a novel pathway involving MYSM1, miR-150, and FLT3, which inhibits B1a cell proliferation and a defect in this pathway may contribute to the pathogenesis of SLE."
27590507
MYSM1 affects RIPK2
| 12 8
MYSM1 binds RIPK2.
| 3 5
RIPK2 binds MYSM1. 5 / 5
| 3 2
sparser
"However, IL-1β treatment weakened the MYSM1-RIPK2 interaction (Fig. xref )."
39746943
reach
"In mouse primary chondrocytes, MYSM1 and RIPK2 interaction was confirmed via co-immunoprecipitation (Fig. 4h), and co-localization of these proteins was observed (Fig. 4i–j)."
39746943
reach
"We observed that PP2Ac interacted with RIPK2, but this interaction was abolished upon IL-1β treatment, and the binding of MYSM1 to RIPK2 was also disrupted under IL-1β stimulation (Fig. 6d, e)."
39746943
reach
"Our study reveals that the deubiquitinase MYSM1 interacts with and deubiquitinates RIPK2, recruiting PP2A to dephosphorylate RIPK2 and ultimately deactivate NF-κB and MAPK signaling pathways, thereby protecting chondrocytes from degeneration."
39746943
sparser
"One of these proteins, Ppp2ca (PP2Ac), functions as a serine/threonine protein phosphatase responsible for a significant portion of eukaryotic protein dephosphorylation events (Fig. xref ). xref The PP2A core enzyme comprises a scaffold subunit (A or PR65 subunit) and a catalytic subunit (C subunit, PP2Ac). xref We observed that PP2Ac interacted with RIPK2, but this interaction was abolished upon IL-1β treatment, and the binding of MYSM1 to RIPK2 was also disrupted under IL-1β stimulation (Fig. xref )."
39746943
RIPK2 binds NOD2 and MYSM1. 2 / 2
| 2
sparser
"To explore further, we assessed by immunoprecipitation the interaction of MYSM1 with the other components in the NOD2:RIP2 complex."
30405132
sparser
"Importantly, LCL-161 did not abolish the enhanced MDP-induced NOD2:RIP2 complex assembly or phosphorylation of RIP2 and IKKα/β caused by MYSM1 deficiency (Supplementary Figure  xref )."
30405132
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 dephosphorylates RIPK2.
| 3 3
MYSM1 dephosphorylates RIPK2. 4 / 4
| 3 1
sparser
"As a deubiquitinase, MYSM1 may not directly dephosphorylate RIPK2; instead, it likely recruits other phosphatases during inflammation."
39746943
reach
"Dephosphorylated and deubiquitinated RIPK2 downstream of MYSM1 maintains articular cartilage homeostasis by antagonizing NF-κB and MAPK signaling in OA.As a deubiquitinase, MYSM1 may not directly dephosphorylate RIPK2; instead, it likely recruits other phosphatases during inflammation."
39746943
reach
"These results suggest that the phosphorylation of RIPK2 is essential for RIPK2 ubiquitination, and MYSM1 reduces the phosphorylation and ubiquitination of RIPK2 with the assistance of PP2A (Fig. 7)."
39746943
reach
"Interestingly, MYSM1 notably enhanced the role of PP2A in dephosphorylating RIPK2, as indicated by the pS176 levels (Fig. 6j, k)."
39746943
MYSM1 dephosphorylates RIPK2 on S176. 2 / 2
| 2
sparser
"Mechanistically, MYSM1 deubiquitinates and dephosphorylates RIPK2 S176 by recruiting PP2A. These findings offer unprecedented evidence of the MYSM1/PP2A/RIPK2 axis’s role in preserving cartilage homeostasis, indicating that targeting MYSM1 and downstream RIPK2 could be therapeutically beneficial for OA treatment."
39746943
sparser
"MYSM1 deubiquitinates and dephosphorylates RIPK2 S176 by recruiting protein phosphatase 2 A (PP2A)."
39746943
MYSM1 deubiquitinates RIPK2.
| 4
MYSM1 deubiquitinates RIPK2. 3 / 3
| 3
reach
"Mechanistically, MYSM1 deubiquitinates and dephosphorylates RIPK2 by recruiting PP2A."
39746943
reach
"These results suggest that the phosphorylation of RIPK2 is essential for RIPK2 ubiquitination, and MYSM1 reduces the phosphorylation and ubiquitination of RIPK2 with the assistance of PP2A (Fig. 7)."
39746943
reach
"Our study reveals that the deubiquitinase MYSM1 interacts with and deubiquitinates RIPK2, recruiting PP2A to dephosphorylate RIPK2 and ultimately deactivate NF-κB and MAPK signaling pathways, thereby protecting chondrocytes from degeneration."
39746943
MYSM1 deubiquitinates RIPK2 on S176. 1 / 1
| 1
reach
"MYSM1 deubiquitinates and dephosphorylates RIPK2 S176 by recruiting protein phosphatase 2 A (PP2A)."
39746943
MYSM1 decreases the amount of RIPK2.
| 1
MYSM1 decreases the amount of RIPK2. 1 / 1
| 1
reach
"Although MYSM1 inhibits the pS176 level of RIPK2, it rapidly increased after exposure to Okadaic Acid (Fig. 6l, m)."
39746943
MYSM1 activates RIPK2.
| 1
MYSM1 activates RIPK2. 1 / 1
| 1
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"Furthermore, overexpression of Mysm1 failed to inhibit phosphorylation at pS176 (Fig. S9A-B) and did not negate the impact of Ripk2 in activating NF-κB and MAPK pathways (Fig. S9C-D)."
39746943
STAT1 affects MYSM1
| 6 13
STAT1 binds MYSM1.
| 5 13
STAT1 binds MYSM1. 10 / 15
| 2 13
sparser
"Besides, we found that oeMYSM1 only promoted the mRNA expression of necroptosis-related genes ( Mlkl / Ripk3 ) rather than other PCD-related genes ( Ripk1 / Fas / Zbp1 / Casp3 ) in H/R-induced HL-1 cells ( xref B-G), indicating that MYSM1-STAT1 axis up-regulated the transcription and expression of necroptosis-related genes."
39897566
sparser
"These findings confirmed a direct interaction between MYSM1 and STAT1, suggesting that STAT1 may be a crucial substrate protein of MYSM1."
39897566
sparser
"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain."
39897566
sparser
"In conclusion, this study illustrated a MYSM1-STAT1 axis in regulating myocardial I/R injury and identified MYSM1 as a pharmacological target for myocardial I/R injury."
39897566
reach
"Therefore, we speculated that MYSM1 may utilize STAT1 as a crucial substrate in the regulation of myocardial I/R injury.Then we examined the direct interaction between MYSM1 and STAT1."
39897566
reach
"These findings confirmed a direct interaction between MYSM1 and STAT1, suggesting that STAT1 may be a crucial substrate protein of MYSM1."
39897566
sparser
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
sparser
"In this study, we demonstrated a specific MYSM1-STAT1 axis in myocardial I/R injury, which might avoid the side effects of targeting STAT1 alone, indicating that targeting MYSM1 could be a more specific therapeutic strategy for myocardial I/R injury."
39897566
sparser
"Apart from the MYSM1-STAT1 interaction, MYSM1 has been reported to deubiquitinate other substrate proteins xref , such as E2A xref , GATA2 xref , and histone H2A xref ."
39897566
sparser
"Then we examined the direct interaction between MYSM1 and STAT1."
39897566
MYSM1 binds STAT1 and SWIRM domain. 2 / 2
| 2
reach
"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
39897566
reach
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
MYSM1 binds STAT1 and MPN domain.According. 1 / 1
| 1
reach
"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain.According to our ubiquitinomics, the K379 residue of STAT1, located in the DNA binding domain (DBD) of STAT1 (Figure 6K), was the most significantly reduced deubiquitination residue of STAT1 after MYSM1 overexpression (Figure 5E)."
39897566
STAT1 inhibits MYSM1.
| 1
STAT1 inhibits MYSM1. 1 / 1
| 1
reach
"Notably, STAT1 knockdown (STAT1-KD, Figure S6J) reversed MYSM1-mediated up-regulation of Mlkl and Ripk3 mRNAs (Figure 7J-K), confirming that MYSM1 transcriptionally increased the expression of necroptosis-related genes via STAT1."
39897566
TRIM21 affects MYSM1
| 8 9
MYSM1 binds TRIM21. 10 / 13
| 4 9
reach
"Consistently, our data indicated that MYSM1 binds to TRIM21 in a SWIRM-dependent manner."
39695708
sparser
"As depicted in Fig.  xref B, it was revealed that the SWIRM domain, but not the SANT or MPN domains, was involved in the interaction between MYSM1 and TRIM21."
39695708
sparser
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig.  xref F)."
39695708
sparser
"Through a Co-IP combined LC-MS/MS analysis, we found that MYSM1 directly interacted with tripartite motif-containing protein 21 (TRIM21)."
39695708
sparser
"Consistently, our data indicated that MYSM1 binds to TRIM21 in a SWIRM-dependent manner."
39695708
sparser
"MYSM1 interacts with TRIM21 to maintain the stability of TRIM21 protein."
39695708
sparser
"We conducted preliminary validation of the LC-MS/MS results and found that the interaction between endogenous MYSM1 and TRIM21 was observed in DOX-treated cardiac tissues (Fig.  xref E) and HL-1 cells (Fig.  xref F)."
39695708
sparser
"These findings confirmed the existence of a direct interaction between MYSM1 and TRIM21."
39695708
reach
"We conducted preliminary validation of the LC-MS/MS results and found that the interaction between endogenous MYSM1 and TRIM21 was observed in DOX-treated cardiac tissues (Fig. 5E) and HL-1 cells (Fig. 5F)."
39695708
sparser
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
| 2
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
MYSM1 binds TRIM21 and MPN domain. 2 / 2
| 2
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
MYSM1 affects ESR
| 17
MYSM1 activates ESR.
| 10
MYSM1 activates ESR. 10 / 10
| 10
reach
"On the other hand, ectopic expression of MYSM1enhanced ERα expression in MCF-7 and HEK293 (Fig. 3D), indicating that MYSM1 up-regulates ERα protein itself at the post-transcriptional level."
38177530
reach
"MYSM1 up-regulated ERα downstream genes, such as c-Myc, VEGF, and CCND1, which play crucial roles in endocrine resistance, suggesting that MYSM1 may act as an ERα co-activator to be involved in conferring endocrine resistance in BCa (Fig. 2)."
38177530
reach
"When cycloheximide (CHX) was applied at specified time points to inhibit protein synthesis, depletion of MYSM1 significantly accelerated ERα degradation and ectopic expression of wild-type MYSM1 plasmid (MYSM1-FL) ameliorated ERα degradation process (Fig. 3C,E)."
38177530
reach
"In addition, the results showed that the association between HATs co-regulatory complex and ERα were diminished after MYSM1 knockdown, suggesting that MYSM1 may potentially associate with the HATs complex to up-regulate ERα action (Fig. 4C)."
38177530
reach
"Consistent with these studies, our results indicate that MYSM1 enhances ERα-induced transactivation by eliminating H2Aub deposition."
38177530
reach
"Taken together, these results demonstrated that MYSM1 depletion suppressed the breast cancer cell growth in mice.Given its pleiotropic biological functions, MYSM1 may be a potential therapeutic target for drug development against breast cancer, especially in endocrine resistant ERα-positive breast cancer."
38177530
reach
"To confirm this result, we further generated transgenic Drosophila with human homolog of CG4751 (MYSM1), the results demonstrated that MYSM1 increased ERα-mediated transactivation, suggesting that MYSM1 may be a co-activator of ERα (Fig. EV1A)."
38177530
reach
"Thus, MYSM1 enhances ERα action via histone and non-histone deubiquitination to promote cell proliferation and antiestrogen insensitivity in breast cancer progression."
38177530
reach
"Meanwhile, MYSM1 knockdown inhibits ERα action in T47D cells, indicating that MYSM1 is a novel co-activator of ERα (Fig. EV2D)."
38177530
reach
"The results showed that MYSM1 simultaneously increased ERα-AF1 and AF2 actions (Figs."
38177530
MYSM1 increases the amount of ESR.
| 4
MYSM1 increases the amount of ESR. 4 / 4
| 4
reach
"The results revealed that loss of MYSM1 dramatically decreased ERα and c-Myc protein expression, accompanied by a reduction in the Ki67 index in xenograft tumor tissue (Figs."
38177530
reach
"The data demonstrated MYSM1 knockdown significantly suppressed the transcription of endogenous ERα regulated genes, including c-Myc, CCND1, VEGF, GREB1, TFF1, E2F1, FOXC1 (Figs."
38177530
reach
"Unexpectedly, in western blot experiments as shown in Fig. 2E,F, we observed that ectopic expression of MYSM1 increased ERα protein expression, while knockdown of MYSM1 reduced ERα protein level."
38177530
reach
"The results showed that knockdown of MYSM1 decreased ERα protein level, whereas ERα mRNA expression had no significant change (Fig. 3A,B)."
38177530
MYSM1 binds ESR.
| 2
MYSM1 binds ESR. 2 / 2
| 2
reach
"Then, we set out to examine the interaction between MYSM1 and ERα in mammalian cells by Co-immunoprecipitation (Co-IP)."
38177530
reach
"The precipitation results demonstrated that loss of SANT (Swi3, Ada2, N-CoR, (TF) IIIB) domain more obviously impaired the association between MYSM1 and ERα, compared with that of SWIRM or MPN domain (Figs."
38177530
MYSM1 deubiquitinates ESR.
| 1
MYSM1 leads to the deubiquitination of ESR. 1 / 1
| 1
reach
"Polyubiquitinated ERα proteins were visibly enriched by immunomagnetic beads in the control group, and MYSM1 depletion significantly enhanced ubiquitination of ERα (Fig. 3H)."
38177530
MYSM1 affects AR
2 | 6 9
MYSM1 binds AR.
2 | 9
MYSM1 binds AR. 10 / 11
2 | 9
sparser
"MYSM1 was able to bind to androgen receptor instead of increasing its expression and knockdown of MYSM1 resulted in activation of Akt/c-Raf/GSK-3β signaling."
31761786
sparser
"MYSM1 interacts with AR and inhibits activation of Akt/c-Raf/GSK-3β signaling in prostate cancer."
31761786
sparser
"Given the repression of AR transactivation by interaction of LCOR with AR [ xref ], we then concentrated on the interaction between MYSM1 and AR to dissect the functional significance of MYSM1 in AR signaling."
31761786
sparser
"To verify the potential interaction between MYSM1 and AR, we carried out protein co-immunoprecipitation (Co-IP) assays with validated antibodies against MYSM1 and AR in C4-2 cells with or without siMYSM1 treatment."
31761786
sparser
"As 2A-DUB may interact with AR via its SWIRM domain ( Metzger et al., 2005 ), recruitment of the complex might be AR dependent."
17707232
sparser
"In summary, these findings demonstrate that MYSM1 can bind to AR and suppress the activation of Akt pathway in CRPC by downregulating phosphorylation levels of Akt, c-Raf and GSK-3β."
31761786
sparser
"Further mechanistic investigation indicated that MYSM1-AR interaction and Akt/c-Raf/GSK-3β pathway may underlie the role of MYSM1 in CRPC progression."
31761786
sparser
"In this work we observed MYSM1-AR interactions which may be also responsible for the role of MYSM1 in androgen-responsive gene transcription."
31761786
biogrid
No evidence text available
31761786
biogrid
No evidence text available
31761786
MYSM1 increases the amount of AR.
| 5
MYSM1 increases the amount of AR. 5 / 5
| 5
reach
"Recent work has shown that 2A-DUB acts as a co-activator of the androgen receptor (AR), modulating full activation of AR dependent gene expression through deubiquitination of H2A."
19489724
reach
"All this highlights the importance of MYSM1 in mammalian development and hematopoiesis, and the biomedical significance of understanding its functions.Monoubiquitinated histone H2A-K119ub was the first MYSM1 substrate to be discovered, and MYSM1 was shown to promote the expression of androgen receptor target genes in prostate cancer cell lines through H2A-K119ub deubiquitination ."
36611064
reach
"2A-DUB, a JAMM family DUB, was found to deubiquitinate H2A and positively regulate transcription of androgen receptor regulated genes in concert with the histone acetylase p and CAF complex [XREF_BIBR]."
23845989
reach
"27 In prostate cancer cells, Mysm1 activates transcription of androgen receptor regulated genes as part of a co and regulatory complex with histone acetyltransferase p300 and CBP associated factor by coordinating histone acetylation and deubiquitination, and by destabilizing the association of linker histone H1 with nucleosomes."
25613381
reach
"MYSM-1 can activate transcription of AR target genes via its involvement with p300 affecting histone acetylation and deubiquitination and binding of H1 to the nucleosome [XREF_BIBR]."
25962961
MYSM1 inhibits AR.
| 1
MYSM1 inhibits AR. 1 / 1
| 1
reach
"In addition, MYSM1 interacts with the AR and reduces AR activity by inhibiting AKT/c-Raf/GSK-3β signaling (78)."
37732538
MYSM1 affects KAT2B
3 | 4 7
MYSM1 binds KAT2B.
3 | 3 7
MYSM1 binds KAT2B. 10 / 14
3 | 3 7
sparser
"2A-DUB interacts with p/CAF in a coregulatory protein complex participating in transcriptional regulation events in androgen receptor-dependent gene activation [46] ."
20685273
biogrid
No evidence text available
17707232
sparser
"Interestingly, 2A-DUB associates with PCAF, a histone acetyltransferase subunit of the hSAGA complex."
21513801
biogrid
No evidence text available
17707232
sparser
"The HAT P/CAF physically interacts with the DUB Mysm1 ( xref ); however, whether DUBs play a role in the regulation of HAT stability is unclear."
28611184
sparser
"The interacting partners 2A-DUB and PCAF, are recruited to the promoter region, remove the uH2A repressive mark and facilitate the dissociation of linker proteins, creating an open chromatin environment."
20974139
sparser
"Coimmunoprecipitation assays further confirmed a specific interaction of 2A-DUB with p/CAF, as well as interactions with R NA- b inding m otif protein 10 (RBM10) and t hyroid hormone r eceptor ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
reach
"2A-DUB interacts with PCAF and is required for full activation of androgen receptor dependent genes."
22649782
reach
"Correlating with the recruitment of 2A-DUB and p/CAF, a clear decrease of uH2A levels and a coincidently increased level of acetylated H3 (Ac-H3) were observed upon DHT treatment in LNCaP cells, sugge[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
reach
"Displacement of linker histones, which is facilitated by their phosphorylation, is generally linked to gene activation but has also been linked to repression in a few instances, coincident with the co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
MYSM1 activates KAT2B.
| 1
MYSM1 activates KAT2B. 1 / 1
| 1
reach
"MYSM1 depletion decreased the recruitment of ERα, pCAF, CBP, and p300 on EREs."
38177530
MYSM1 activates DNA-templated transcription. 10 / 14
| 10 4
reach
"MYSM1 deubiquitinates monoubiquitinated histone H2A and directs the recruitment of transcription factors to the promoter of target genes, including EBF1 and PU.1."
27590507
sparser
"Mechanistic studies revealed that MYSM1 activates Pax5 transcription by coordinating histone modifications and directing the recruitment of the transcription activators such as PU.1 to the Pax5 locus in B cells."
26348977
reach
"MYSM1 stimulates transcription of miR-150 in B1a cells with c-Myc."
27590507
sparser
"The histone H2A deubiquitinase 2A-DUB or Mysm1/Kiaa1915 (Myb-like SWIRM and MPN domain containing1) is a nuclear protein of 828 amino acids containing a SWIRM domain, a SANT (SWI-SNF, ADA N-CoR, TFIIIB) domain with DNA-binding activity and a JAMN/MPN domain with intrinsic metalloprotease-like activity. xref In prostate cancer cells, Mysm1 activates transcription of androgen receptor-regulated genes as part of a co-regulatory complex with histone acetyltransferase p300/CBP-associated factor by coordinating histone acetylation and deubiquitination, and by destabilizing the association of linker histone H1 with nucleosomes. xref More recently, analysis of Mysm1 knockout mice revealed additional functions of the H2A-DUB in regulation of hematopoietic development as Mysm1 deficiency resulted in – among other abnormalities – severe depletion of B cells and T cells, anemia, and thrombocytosis. xref , xref Comparable with the pathophysiology of mice lacking PRC1 component Bmi1, xref postnatal lymphopenia in Mysm1-deficient mice correlated with a depletion of HSC likely resulting from activation of the DDR and uncontrolled production of reactive oxygen species (ROS). xref In murine skin, Mysm1 deficiency was associated with atrophy (present article and own unpublished observations) and malformation of the tail. xref "
25613381
sparser
"MYSM1 and USP16 activate transcription."
18226187
reach
"MYSM1 as a deubiquitinase is involved in the deubiquitination of histone H2A to enhance gene transcription (Fiore et al, 2020)."
38177530
reach
"MYSM1 excludes monoubiquitin from H2AK119ub1 and activates transcription in cooperation with histone acetylation [90]."
37580737
reach
"As a deubiquitinase, MYSM1 stabilizes ERα protein through its MPN enzyme catalytic domain and is recruited with ERα at the promoters of E2-induced genes, thereby triggering transcription initiation and subsequent acceleration in cell proliferation and suppression of antiestrogen sensitivity."
38177530
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"Additionally, MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1.Conclusion: This study illustrated a MYSM1-STAT1 axis in regulating myocardial I/R injury and identified MYSM1 as a pharmacological target for myocardial I/R injury."
39897566
reach
"In summary, these results showed that MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1."
39897566
MYSM1 affects Cardiotoxicity
| 14
MYSM1 activates Cardiotoxicity.
| 10
reach
"MYSM1 mediates ferroptosis in DOX-induced cardiotoxicity."
39695708
reach
"In this research, through a comparison of RNA sequencing (RNA-seq) databases and functional screening of the JAMMs, we identified Myb-like, SWIRM, and MPN domains 1 (MYSM1) as a potential regulator of DOX-induced cardiotoxicity."
39695708
reach
"To further verify the involvement of ferroptosis in MYSM1-mediated DOX-induced cardiotoxicity, the ferroptosis inhibitor ferrostatin-1 (Fer-1) was administered to HL-1 cells before DOX treatment."
39695708
reach
"Here, we performed an IP combined with LC-MS/MS analysis and identified TRIM21 as a direct substrate protein of MYSM1 in DOX-induced cardiotoxicity."
39695708
reach
"In this study, we aimed to explore the function and regulatory mechanisms of MYSM1 in DOX-induced cardiotoxicity."
39695708
reach
"Furthermore, MYSM1 exacerbated DOX-induced cardiotoxicity by enhancing ferroptosis."
39695708
reach
"Up to now, the potential role and mechanism of MYSM1 in DOX-induced cardiotoxicity have not yet been revealed.The purpose of this study was to clarify MYSM1’s regulatory function and mechanism in DOX-induced cardiotoxicity."
39695708
reach
"Furthermore, MYSM1 exacerbated DOX-induced cardiotoxicity by increasing ferroptosis signaling pathway activity."
39695708
reach
"PI staining confirmed that the ferroptosis inhibitor Fer-1 alleviated the cardiotoxicity induced by MYSM1 overexpression (Fig. 7Q-R)."
39695708
reach
"Identification of MYSM1 as a regulator of DOX-induced cardiotoxicity."
39695708
MYSM1 inhibits Cardiotoxicity.
| 4
MYSM1 inhibits Cardiotoxicity. 4 / 4
| 4
reach
"These results suggested that knockdown of MYSM1 alleviated DOX-induced cardiotoxicity."
39695708
reach
"MYSM1 alleviates DNA damage and inhibits cellular senescence [11, 13], which is associated with DOX-induced cardiotoxicity [14]."
39695708
reach
"Correspondingly, cardiomyocyte-specific knockdown of MYSM1 by AAV9 protected the heart from DOX-induced cardiotoxicity."
39695708
reach
"Knockdown of MYSM1 significantly alleviated DOX-induced cardiotoxicity, including cardiac dysfunction, myocardial interstitial fibrosis, and cardiomyocyte death."
39695708
KAT2B affects MYSM1
3 | 3 7
MYSM1 binds KAT2B. 10 / 14
3 | 3 7
sparser
"2A-DUB interacts with p/CAF in a coregulatory protein complex participating in transcriptional regulation events in androgen receptor-dependent gene activation [46] ."
20685273
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No evidence text available
17707232
sparser
"Interestingly, 2A-DUB associates with PCAF, a histone acetyltransferase subunit of the hSAGA complex."
21513801
biogrid
No evidence text available
17707232
sparser
"The HAT P/CAF physically interacts with the DUB Mysm1 ( xref ); however, whether DUBs play a role in the regulation of HAT stability is unclear."
28611184
sparser
"The interacting partners 2A-DUB and PCAF, are recruited to the promoter region, remove the uH2A repressive mark and facilitate the dissociation of linker proteins, creating an open chromatin environment."
20974139
sparser
"Coimmunoprecipitation assays further confirmed a specific interaction of 2A-DUB with p/CAF, as well as interactions with R NA- b inding m otif protein 10 (RBM10) and t hyroid hormone r eceptor ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
reach
"2A-DUB interacts with PCAF and is required for full activation of androgen receptor dependent genes."
22649782
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"Correlating with the recruitment of 2A-DUB and p/CAF, a clear decrease of uH2A levels and a coincidently increased level of acetylated H3 (Ac-H3) were observed upon DHT treatment in LNCaP cells, sugge[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
reach
"Displacement of linker histones, which is facilitated by their phosphorylation, is generally linked to gene activation but has also been linked to repression in a few instances, coincident with the co[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
MYSM1 affects MYC
| 9 2
MYSM1 binds MYC.
| 5 2
MYSM1 binds MYC. 7 / 7
| 5 2
sparser
"Meanwhile, co-Immunoprecipitation (co-IP) was performed to detect the interaction between MYSM1 and c-Myc and results showed that MYSM1 protein co-immunoprecipitated with c-Myc in murine RAW264.7 cells (Figure xref )."
27590507
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"Here, we show that MYSM1 and c-Myc bind to the same region (approximately 1200–1000 nt upstream) of the promoter of miR-150 in B1a cells from WT mice but c-Myc cannot bind to the promoter in MYSM1 −/− mice."
27590507
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"Overall, this suggested a possible cooperation between cMYC and MYSM1 in the transcriptional regulation of genes encoding ribosomal proteins and translation factors.We have previously validated MYSM1 binding to the promoters of RP genes in Ba/F3 cells by ChIP‐qPCR, and also demonstrated a reduction in RP‐gene expression in Mysm1‐shRNA knockdown Ba/F3 cells and in Mysm1‐deficient primary haematopoietic stem and progenitor cells."
34114734
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"10 To further validate the overlap in the genomic binding sites of MYSM1 and cMYC in Ba/F3 cells, the binding of cMYC at the MYSM1‐binding sites of select genes encoding RPs and translation factors was tested and confirmed with ChIP‐qPCR (Figure 1E, Supporting Information S1)."
34114734
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"The binding of MYSM1 and cMYC at the shared sites was also confirmed in cells derived from EuMyc mouse B cell lymphoma, 14 , 15 specifically for the Rpl7 and Eef1g gene promoters (data not shown)."
34114734
reach
"Overall, we demonstrate shared DNA binding of cMYC and MYSM1 at the promoters of genes encoding ribosomal proteins and translation factors."
34114734
reach
"Meanwhile, co-Immunoprecipitation (co-IP) was performed to detect the interaction between MYSM1 and c-Myc and results showed that MYSM1 protein co-immunoprecipitated with c-Myc in murine RAW264.7 cells."
27590507
MYSM1 activates MYC.
| 3
MYSM1 activates MYC. 3 / 3
| 3
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"This firmly establishes that loss of MYSM1 inhibits the oncogenic activity of cMYC, and protects against B cell lymphoma onset and progression in mouse models.To understand the mechanisms underlying the protective activity of MYSM1‐loss in B cell lymphoma, EuMyc tumours were harvested from Mysm1 and control mice, and lymphoma cells isolated by cell‐sorting as live B220 cells, for ex vivo analyses with qRT‐PCR and intracellular flow cytometry."
34114734
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"Loss of MYSM1 inhibits the oncogenic activity of cMYC in B cell lymphoma."
34114734
reach
"This indicates that the loss of MYSM1 can inhibit the oncogenic activity of cMYC and delay the onset of fatal lymphoma.To further establish the protective effect of MYSM1‐loss on lymphoma disease progression, we employed an adoptive lymphoma cell transfer model."
34114734
MYSM1 increases the amount of MYC.
| 1
MYSM1 increases the amount of MYC. 1 / 1
| 1
reach
"The results revealed that loss of MYSM1 dramatically decreased ERα and c-Myc protein expression, accompanied by a reduction in the Ki67 index in xenograft tumor tissue (Figs."
38177530
AR affects MYSM1
2 | 9
MYSM1 binds AR. 10 / 11
2 | 9
sparser
"MYSM1 was able to bind to androgen receptor instead of increasing its expression and knockdown of MYSM1 resulted in activation of Akt/c-Raf/GSK-3β signaling."
31761786
sparser
"MYSM1 interacts with AR and inhibits activation of Akt/c-Raf/GSK-3β signaling in prostate cancer."
31761786
sparser
"Given the repression of AR transactivation by interaction of LCOR with AR [ xref ], we then concentrated on the interaction between MYSM1 and AR to dissect the functional significance of MYSM1 in AR signaling."
31761786
sparser
"To verify the potential interaction between MYSM1 and AR, we carried out protein co-immunoprecipitation (Co-IP) assays with validated antibodies against MYSM1 and AR in C4-2 cells with or without siMYSM1 treatment."
31761786
sparser
"As 2A-DUB may interact with AR via its SWIRM domain ( Metzger et al., 2005 ), recruitment of the complex might be AR dependent."
17707232
sparser
"In summary, these findings demonstrate that MYSM1 can bind to AR and suppress the activation of Akt pathway in CRPC by downregulating phosphorylation levels of Akt, c-Raf and GSK-3β."
31761786
sparser
"Further mechanistic investigation indicated that MYSM1-AR interaction and Akt/c-Raf/GSK-3β pathway may underlie the role of MYSM1 in CRPC progression."
31761786
sparser
"In this work we observed MYSM1-AR interactions which may be also responsible for the role of MYSM1 in androgen-responsive gene transcription."
31761786
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No evidence text available
31761786
biogrid
No evidence text available
31761786
RIPK2 affects MYSM1
| 5 5
RIPK2 binds MYSM1.
| 3 5
RIPK2 binds MYSM1. 5 / 5
| 3 2
sparser
"However, IL-1β treatment weakened the MYSM1-RIPK2 interaction (Fig. xref )."
39746943
reach
"In mouse primary chondrocytes, MYSM1 and RIPK2 interaction was confirmed via co-immunoprecipitation (Fig. 4h), and co-localization of these proteins was observed (Fig. 4i–j)."
39746943
reach
"We observed that PP2Ac interacted with RIPK2, but this interaction was abolished upon IL-1β treatment, and the binding of MYSM1 to RIPK2 was also disrupted under IL-1β stimulation (Fig. 6d, e)."
39746943
reach
"Our study reveals that the deubiquitinase MYSM1 interacts with and deubiquitinates RIPK2, recruiting PP2A to dephosphorylate RIPK2 and ultimately deactivate NF-κB and MAPK signaling pathways, thereby protecting chondrocytes from degeneration."
39746943
sparser
"One of these proteins, Ppp2ca (PP2Ac), functions as a serine/threonine protein phosphatase responsible for a significant portion of eukaryotic protein dephosphorylation events (Fig. xref ). xref The PP2A core enzyme comprises a scaffold subunit (A or PR65 subunit) and a catalytic subunit (C subunit, PP2Ac). xref We observed that PP2Ac interacted with RIPK2, but this interaction was abolished upon IL-1β treatment, and the binding of MYSM1 to RIPK2 was also disrupted under IL-1β stimulation (Fig. xref )."
39746943
RIPK2 binds NOD2 and MYSM1. 2 / 2
| 2
sparser
"To explore further, we assessed by immunoprecipitation the interaction of MYSM1 with the other components in the NOD2:RIP2 complex."
30405132
sparser
"Importantly, LCL-161 did not abolish the enhanced MDP-induced NOD2:RIP2 complex assembly or phosphorylation of RIP2 and IKKα/β caused by MYSM1 deficiency (Supplementary Figure  xref )."
30405132
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
RIPK2 inhibits MYSM1.
| 2
RIPK2 inhibits MYSM1. 1 / 1
| 1
reach
"The Ripk2 S176D mutation accelerated OA pathogenesis, while Ripk2 silencing or Ripk2 S176A mutation deactivated NF-kappaB and MAPK pathways, counteracting the role of MYSM1."
39746943
RIPK2-S176A inhibits MYSM1. 1 / 1
| 1
reach
"The Ripk2 S176D mutation accelerated OA pathogenesis, while Ripk2 silencing or Ripk2 S176A mutation deactivated NF-kappaB and MAPK pathways, counteracting the role of MYSM1."
39746943
MYSM1 affects ferroptosis
| 10
MYSM1 activates ferroptosis.
| 7
MYSM1 activates ferroptosis. 7 / 7
| 7
reach
"Furthermore, MYSM1 mediated ferroptosis in cardiomyocytes to promote DOX-induced cardiotoxicity.A single zinc metalloprotease family is represented by the JAMMs family, whereas the remaining DUB families belong to the cysteine peptidases family [4]."
39695708
reach
"We have thus far focused on the compromised HSC function due to MYSM1 deficiency causing reduced protein synthesis and increased ferroptosis, as a result."
36803603
reach
"Consequently, we propose that MYSM1 may serve as a more viable therapeutic target than TRIM21 for addressing DOX-induced cardiotoxicity.In conclusion, our results showed that MYSM1 plays a deubiquitination role in regulating TRIM21 stability and mediating DOX-induced cardiomyocyte ferroptosis."
39695708
reach
"Furthermore, MYSM1 exacerbated DOX-induced cardiotoxicity by enhancing ferroptosis."
39695708
reach
"MYSM1 mediates ferroptosis in DOX-induced cardiotoxicity."
39695708
reach
"Knockdown of MYSM1 induced an increase in Nrf2 translocation from the cytosol to the nucleus (Fig. 7K), whereas overexpression of MYSM1 reversed this alteration (Fig. 7L), suggesting that MYSM1 may mediate ferroptosis through Nrf2."
39695708
reach
"Recent studies demonstrated that MYSM1 deficiency causes human hematopoietic stem cell loss by ferroptosis, highlighting the broader developmental and regenerative role of ferroptosis (Zhao et al., 2023)."
37991825
MYSM1 inhibits ferroptosis.
| 3
MYSM1 inhibits ferroptosis. 3 / 3
| 3
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"Knockdown of MYSM1 induced an increase in Nrf2 translocation from the cytosol to the nucleus (Fig. 7K), whereas overexpression of MYSM1 reversed this alteration (Fig. 7L), suggesting that MYSM1 may mediate ferroptosis through Nrf2."
39695708
reach
"4 , 6 The recent demonstration that MYSM1 deficiency causes proteostatic stress and ferroptosis in HSCs could explain why SGR provided such a benefit in patient' HSCs."
39233474
reach
"Recent studies have found that the loss of histone deubiquitinase MYSM1 can induce ferroptosis and affect the function of hematopoietic stem cell, providing a new clinical approach for treating complex blood diseases (Zhao et al., 2023)."
39834804
MYSM1 inhibits cell differentiation.
| 9
reach
"MYSM1 negatively regulates differentiation of human B cells to plasma cells."
31607324
reach
"Mysm1 knockdown in NSCs leads to NSC proliferation and early differentiation into neurons."
38342917
reach
"We demonstrated that MYSM1 intrinsically represses plasma cell differentiation and antibody production."
26348977
reach
"We revealed that MYSM1 intrinsically represses plasma cell differentiation and antibody production."
26348977
reach
"Mechanistic studies revealed that MYSM1 intrinsically represses plasma cell differentiation and antibody production by activating the transcription of Pax5, the repressors of plasma cell differentiation, in mature B cells."
26348977
reach
"Systemic loss of Mysm1 in Mysm1 fl/fl Tg.CreERT2 mice also impaired lymphoid lineage differentiation, resulting in a depletion of common lymphoid progenitors (CLPs), as well as mature B cells and CD8 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27833034
reach
"MYSM1 intrinsically represses plasma cell differentiation."
26348977
reach
"Taken together, these data demonstrate that MYSM1 is an intrinsic repressor of plasma cell differentiation."
26348977
reach
"Thus, these data demonstrate that MYSM1 represses plasma cell differentiation, as well as Ig production by plasma cells."
26348977
MYSM1 activates cell differentiation.
| 1
MYSM1 activates cell differentiation. 1 / 1
| 1
reach
"XREF_FIG shows that forced expression of MYSM1, but not control, rescued the enhanced plasma cell differentiation of Mysm1 -/- B cells."
26348977
MYSM1 affects apoptotic process
| 2 7
MYSM1 inhibits apoptotic process.
| 1 5
MYSM1 inhibits apoptotic process. 6 / 6
| 1 5
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"The knockdown of Mysm1 shifts HSCs from a quiescent state to a rapid cycle and increases their apoptosis rate, which leads to the depletion of the stem cell pool and impairs the self-renewal and lineage reconstruction abilities of Mysm1-deficient mice [13]."
38342917
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"Mechanistically, defective development of Mysm1-deficient mice correlated with altered gene transcription, increased apoptosis, and increased DNA damage in a variety of tissues, and was largely rescued by concomitant deletion of tumor suppressor p53 [38,42,43,44]."
32466590
eidos
"Higher numbers of TUNEL-positive cells were observed in the liver of AAV9-Ctrl-treated mice compared to AAV9-Mysm1-treated mice ( Figure S11A , Supporting Information ) , confirming that MYSM1 suppresses DNA damage-induced senescence and apoptosis ."
33240758
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"Mysm1 deletion promoted NSC proliferation and apoptosis, resulting in depletion of the stem cell pool."
38342917
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"Mysm1 knockdown promotes the proliferation and increases susceptibility to apoptosis of NSCs in vitro."
38342917
reach
"MYSM1-deficient cells have increased p38 activation, cell cycle arrest, and increased apoptosis, presumably due to altered transcriptional activity."
29731008
MYSM1 activates apoptotic process.
| 1 2
reach
"MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway."
35217648
eidos
"MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway ."
35217648
reach
"In addition, another study confirmed that the deletion MYSM1 restores dormant HSCs to a cyclic state and enhances apoptosis of HSCs, leading to depletion in the stem cell pool through blocked recruitment of the transcription factors GATA2 and RUNX1 to the GFI1 locus [XREF_BIBR]."
33327527
MYSM1 affects translation
| 8
MYSM1 activates translation. 8 / 8
| 8
reach
"We have thus far focused on the compromised HSC function due to MYSM1 deficiency causing reduced protein synthesis and increased ferroptosis, as a result."
36803603
reach
"Given that prior studies have shown that HSCs have a characteristically low level of protein synthesis and we have demonstrated how reduced protein synthesis due to MYSM1 loss can induce ferroptosis, we wondered if overexpression of MYSM1 could augment protein synthesis in healthy HSCs and thereby protect the cells from ferroptosis."
36803603
reach
"When cycloheximide (CHX) was applied at specified time points to inhibit protein synthesis, depletion of MYSM1 significantly accelerated ERα degradation and ectopic expression of wild-type MYSM1 plasmid (MYSM1-FL) ameliorated ERα degradation process (Fig. 3C,E)."
38177530
reach
"Another research has reported that the loss of the histone deubiquitinase Myb-Like SWIRM and MPN domains 1 (MYSM1) increased the sensitivity of HSCs to ferroptosis by reducing protein synthesis rates in HSCs [29]."
39039566
reach
"Indeed, the latest evidence suggests that human cord blood HSCs are more susceptible to ferroptosis than downstream progenitor cells ex vivo due to a low level of antioxidant protein synthesis, and MY[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38582294
reach
"In hematopoietic stem cells (HSC), histone deubiquitinase MYSM1 was shown to mediate the vulnerabilities of HSC to ferroptosis stress by regulating protein synthesis."
39762627
reach
"Recently, Zhao et al. demonstrated that histone deubiquitinase MYSM1 (Myb-like, SWIRM and MPN domains 1) deficiency reduced the translation of ferroptosis-protective mRNAs, resulting in increased ferroptosis of human hematopoietic stem cells (HSCs), and HSC population maintenance was fully restored by the ferroptosis inhibitors (e.g., deferoxamine (DFO), ferrostatin-1 (Fer-1), and vitamin E)16."
37497000
reach
"Overall, we demonstrate that the loss of MYSM1 in mouse B cell lymphoma represses the induction of ribosomal protein genes, reduces cellular protein synthesis rate, promotes p53 activation and potently inhibits cMYC oncogenic functions.To compare the location of the genomic binding sites of MYSM1 and cMYC, we consolidated the ChIP‐Seq datasets for cMYC and its dimerization partner MAX from multipotent haematopoietic progenitor cells HPC7 12 , 13 with the MYSM1 ChIP‐Seq acquired in our recent work in a B cell progenitor cell line Ba/F3."
34114734
MYSM1 affects PAX5
| 8
MYSM1 increases the amount of PAX5.
| 4
MYSM1 increases the amount of PAX5. 4 / 4
| 4
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"MYSM1 was shown to promote expression of Ebf1, Pax5, and Id2, key transcriptional regulators of B-cell commitment and differentiation."
38065233
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"Similarly, in mouse hematopoietic stem or progenitor cells (HSPCs) MYSM1 was shown to promote the expression of Gfi1, Flt3, Id2, Ebf1 and Pax5 genes, important for the normal progression of hematopoiesis, also via H2A-K119ub deubiquitination of their promoters and other regulatory elements ."
36611064
reach
"Mechanistic studies revealed that MYSM1 activates Pax5 transcription by coordinating histone modifications and directing the recruitment of the transcription activators such as PU.1 to the Pax5 locus in B cells."
26348977
reach
"Further, we tested the possibility that MYSM1 may directly activate the transcription of Pax5 in mature B cells as well."
26348977
MYSM1 activates PAX5.
| 3
MYSM1 activates PAX5. 2 / 2
| 2
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"Mechanistic studies revealed that MYSM1 is a transcriptional activator of Pax5, the repressors of plasma cell differentiation, by facilitating key transcriptional factor recruitment and coordinating histone modifications at the Pax5 locus in B cells."
26348977
reach
"Mechanistic studies demonstrated that MYSM1 is a transcriptional activator of Pax5, the repressors of plasma cell differentiation, by facilitating key transcriptional factor recruitment and coordinating histone modifications at the Pax5 loci."
26348977
Modified MYSM1 activates PAX5. 1 / 1
| 1
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"MYSM1 expression activated transcription of the Ebf1alpha promoter and modestly activated the Ebf1beta promoter, but only marginally activated the Pax5 promoter and Cd79a promoter."
22169041
MYSM1 decreases the amount of PAX5.
| 1
MYSM1 decreases the amount of PAX5. 1 / 1
| 1
reach
"Altogether, MYSM1 was proposed to inhibit plasma cell differentiation by maintaining Pax5 expression, via histone H2AK119 deubiquitination and recruitment of transcription factor PU.1 to the Pax5 locus [XREF_BIBR] (XREF_FIG)."
32344625
MYSM1 affects Histone
| 4 4
MYSM1 binds Histone.
| 4
MYSM1 binds Histone. 4 / 4
| 4
sparser
"The SWIRM domain of MYSM1, in contrast, does not have direct DNA binding activity [ xref ], and although it is a common domain-type for chromatin-associated proteins [ xref , xref ], it was shown to be dispensable for MYSM1 interactions with histones, at least in some systems [ xref ]."
32344625
sparser
"The SANT domain of MYSM1 binds to DNA and is crucial for MYSM1’s binding to histones [ xref ]."
39695708
sparser
"These data suggest that MYSM1 interacts with other histone modifiers to regulate chromatin status at the Ebf1 locus."
22169041
sparser
"The SANT domain of MYSM1 can bind to DNA and is required for MYSM1 to bind to histones xref , xref ."
39897566
MYSM1 inhibits Histone.
| 2
MYSM1 inhibits Histone. 2 / 2
| 2
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"53 Our work shows that MYSM1 represses p53 stress response genes by antagonizing other histone modifications (H3K27ac and H3K4me3)."
26768662
reach
"The gamma-H2A histone family member X (𝛾 -H2AX) protein, a marker of DNA damage, is initially triggered by DOX treatment and suppressed by MYSM1 in lymphocytes [13]."
39695708
MYSM1 decreases the amount of Histone.
| 1
MYSM1 decreases the amount of Histone. 1 / 1
| 1
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"Importantly, MYSM1 knockdown enhanced histone H2Aub level, while H2Bub1 level had no significant change."
38177530
MYSM1 activates Histone.
| 1
MYSM1 activates Histone. 1 / 1
| 1
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"Moreover, the results showed that MYSM1 depletion inhibited H3K4me3, H3K9ac and H3K27ac levels on ERE regions, suggesting that MYSM1 may crosstalk with the HATs complex to modulate histone modifications on ERE regions of ERα-regulatory genes in BCa-derived cells (Fig. 4D; Appendix Fig. S2A–C)."
38177530
MYSM1 affects ATP
| 8
MYSM1 inhibits ATP.
| 3
MYSM1 inhibits ATP. 3 / 3
| 3
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"These results suggest that Mysm1 knockdown in astrocytes may upregulate TCA cycle process and mitochondrial oxidative phosphorylation levels to enhance mitochondrial function.Combined with the results that Mysm1 knockdown in astrocytes in vitro could produce more ATP, we explored whether Mysm1 knockdown in vivo could increase ATP in the interstitial space of the brain."
36414403
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"Here, we found that astrocytic Mysm1 knockdown in MHb cells promoted ATP production and induced antidepressant effects, which further confirmed the important role of astrocytes in energy modulation."
36414403
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"These results suggest that Mysm1 exerts antidepressant effects by affecting ATP levels.Given that energy production in astrocytes is largely dependent on mitochondrial oxidative metabolism in response to neuronal activity, 20 we next tested whether Mysm1 knockdown in astrocytes could produce more ATP."
36414403
MYSM1 phosphorylates ATP.
| 2
MYSM1 leads to the phosphorylation of ATP. 2 / 2
| 2
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"These results revealed that Mysm1 accumulation in astrocytes was involved in murine depressive‐like disorders.2.4 Downregulation of Mysm1 Enhanced Mitochondrial Oxidative Phosphorylation and ATP Levels in Astrocytes and Improved Mitochondrial Structure, Alleviating Depression-Like Behaviors in Mice."
36414403
reach
"These results suggested that downregulation of Mysm1 enhances mitochondrial oxidative phosphorylation and ATP levels in astrocytes and improves mitochondrial structure.Astrocytes can actively release ATP from vesicles or lysosomes in a calcium‐dependent manner."
36414403
MYSM1 increases the amount of ATP.
| 2
MYSM1 increases the amount of ATP. 2 / 2
| 2
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"These results suggested that downregulation of Mysm1 enhances mitochondrial oxidative phosphorylation and ATP levels in astrocytes and improves mitochondrial structure.Astrocytes can actively release ATP from vesicles or lysosomes in a calcium‐dependent manner."
36414403
reach
"These results revealed that Mysm1 accumulation in astrocytes was involved in murine depressive‐like disorders.2.4 Downregulation of Mysm1 Enhanced Mitochondrial Oxidative Phosphorylation and ATP Levels in Astrocytes and Improved Mitochondrial Structure, Alleviating Depression-Like Behaviors in Mice."
36414403
MYSM1 activates ATP.
| 1
MYSM1 activates ATP. 1 / 1
| 1
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"Suppression of Mysm1 expression could relieve depressive disorders by promoting ATP production."
36414403
Tamoxifen affects MYSM1
| 6 1
Tamoxifen activates MYSM1.
| 5
Tamoxifen activates MYSM1. 5 / 5
| 5
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"For tamoxifen induced Mysm1 gene inactivation in the Mysm1 flox and flox Gt (ROSA) 26Sor tm1 (cre and ERT2) mouse line, the mice were injected intraperitoneally with tamoxifen (T5648, Sigma, St. Louis, MO, USA) in sterilized corn oil (Sigma C8267) at 0.15 mg/g per injection, with eight doses in total administered over 16 days."
26768662
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"For this purpose, wild-type bone marrow was transplanted into Mysm1 fl/fl Tg.CreERT2 recipients, followed by tamoxifen-induced ablation of Mysm1 specifically within non-hematopoietic cells of the reci[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27833034
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"The Mysm1 fl/fl line was generated as previously described [13] and crossed to the Gt(ROSA)26Sor tm1(cre/ERT2) line for tamoxifen-induced Mysm1 inactivation."
27833034
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"Mysm1 flox and flox line was generated by crossing Mysm1 tm1a mice to the Gt (ROSA) 26 Sor tm1 (FLP1) Dym and Wtsi line with ubiquitous expression of Flp recombinase, 56 and subsequently bred to Gt (ROSA) 26Sor tm1 (cre and ERT2) mice for tamoxifen induced Mysm1 inactivation."
26768662
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"14 The EuMyc mouse line was crossed to our established Mysm1 and Mysm1 Cre mouse lines, allowing either constitutive or tamoxifen‐induced Mysm1‐deletion."
34114734
Tamoxifen inhibits MYSM1.
| 1 1
Tamoxifen inhibits MYSM1. 2 / 2
| 1 1
sparser
"The Mysm1 fl/fl line was generated as previously described [13] and crossed to the Gt(ROSA)26Sor tm1(cre/ERT2) line for tamoxifen-induced Mysm1 inactivation."
27833034
reach
"For tamoxifen-induced Mysm1-gene deletion, mice of Mysm1 Cre and control genotypes were injected intraperitoneally with tamoxifen (Sigma-Aldrich, T5648) in sterilized corn oil at 0.12 mg per gram body weight per injection, with 8 doses administered in total over 16 days, as in our previous work ."
36611064
Acetylsalicylic acid affects MYSM1
| 6 1
Acetylsalicylic acid decreases the amount of MYSM1.
| 5
Acetylsalicylic acid decreases the amount of MYSM1. 5 / 5
| 5
reach
"26 Western blot data revealed that astrocytic Mysm1 protein levels were significantly decreased by aspirin treatment in a dose‐dependent manner (Figure  7A)."
36414403
reach
"Aspirin administration, reduced the protein expression of Mysm1, increased the protein expression of H2AK119-Ub and thereby reduced the Set7 protein expression in glomeruli isolated from diabetic animals and prevented renal fibrosis."
29656179
reach
"Aspirin was found to inhibit Mysm1 expression, and the injection of aspirin showed antidepressant effects."
36414403
reach
"Previous studies report that aspirin can reduce the expression of Mysm1 in the glomeruli of diabetic animals."
36414403
reach
"A previous report in support of our data demonstrated that aspirin was able to suppress Mysm1 expression in diabetic animals."
36414403
Acetylsalicylic acid inhibits MYSM1.
| 1
Acetylsalicylic acid inhibits MYSM1. 1 / 1
| 1
sparser
"Moreover, the pharmacological inhibition of astrocytic Mysm1 by aspirin relieved depressive‐like behaviors in mice."
36414403
Acetylsalicylic acid increases the amount of MYSM1.
| 1
Acetylsalicylic acid increases the amount of MYSM1. 1 / 1
| 1
reach
"Aspirin reduced renal fibrosis in diabetic rats by decreasing Mysm1 expression, boosting H2AK119-Ub expression, and lowering the Set7 protein expression [ 72 ]."
35985560
MYSM1 is modified
7 |
MYSM1 is phosphorylated on S218. 2 / 2
2 |
hprd
No evidence text available
18669648
hprd
No evidence text available
17525332
MYSM1 is phosphorylated on S234. 2 / 2
2 |
hprd
No evidence text available
18669648
hprd
No evidence text available
17525332
MYSM1 is phosphorylated on S267. 2 / 2
2 |
hprd
No evidence text available
20068231
hprd
No evidence text available
17525332
MYSM1 is phosphorylated on S110. 1 / 1
1 |
hprd
No evidence text available
20068231
MYSM1 activates Neoplasm Invasiveness.
| 4
reach
"MYSM1 silencing decreases migration and invasion in CRC SW480 cells."
28498834
reach
"In the present study, we knocked down MYSM1 expression by transfecting siRNA for MYSM1 to CRC SW480 cells and found that MYSM1 silencing inhibited the migration and invasion of CRC cells, indicating that MYSM1 expression was positively associated with cell migration and invasion, which was identical to the clinicopathologic characteristics."
28498834
reach
"Scratch assay and Transwell assay showed that MYSM1 silencing decreased migration and invasion abilities of SW480 cells."
28498834
reach
"Transwell assay and Scratch assay indicated that MYSM1 silencing markedly inhibited cellular invasion and migration in SW480 cells (XREF_FIG)."
28498834
MYSM1 inhibits Neoplasm Invasiveness. 3 / 3
| 3
reach
"It was found that MYSM-1 could suppress the proliferation, migration, and invasion of renal carcinoma cells."
26409454
reach
"MYSM-1 suppresses migration and invasion in renal carcinoma through inhibiting epithelial-mesenchymal transition."
26409454
reach
"These data exhibited the correlation between MYSM1 and CRC malignancy involving cell metastasis ability but not cell proliferation ability.However, it has been reported that MYSM1 suppressed cellular migration and invasion in renal carcinoma by inhibiting epithelial-mesenchymal transition [XREF_BIBR]."
28498834
MYSM1 affects ESR1
4 | 1 2
MYSM1 binds ESR1. 7 / 7
4 | 1 2
biogrid
No evidence text available
38177530
biogrid
No evidence text available
38177530
biogrid
No evidence text available
38177530
biogrid
No evidence text available
38177530
reach
"MYSM1 physically associates with ERalpha in breast cancer cells."
38177530
sparser
"Virtual screening identified Imatinib as a small molecule capable of interacting with the catalytic MPN domain of MYSM1, and this effectively inhibited breast cancer cell growth, highlighting the MYSM1-ERα axis as a promising target to overcome endocrine independence in breast cancer [ xref ]."
| PMC
sparser
"A virtual screen shows that the small molecule Imatinib could potentially interact with catalytic MPN domain of MYSM1 to inhibit BCa cell growth via MYSM1-ERα axis."
38177530
MYC affects MYSM1
| 5 2
MYSM1 binds MYC. 7 / 7
| 5 2
sparser
"Meanwhile, co-Immunoprecipitation (co-IP) was performed to detect the interaction between MYSM1 and c-Myc and results showed that MYSM1 protein co-immunoprecipitated with c-Myc in murine RAW264.7 cells (Figure xref )."
27590507
sparser
"Here, we show that MYSM1 and c-Myc bind to the same region (approximately 1200–1000 nt upstream) of the promoter of miR-150 in B1a cells from WT mice but c-Myc cannot bind to the promoter in MYSM1 −/− mice."
27590507
reach
"Overall, this suggested a possible cooperation between cMYC and MYSM1 in the transcriptional regulation of genes encoding ribosomal proteins and translation factors.We have previously validated MYSM1 binding to the promoters of RP genes in Ba/F3 cells by ChIP‐qPCR, and also demonstrated a reduction in RP‐gene expression in Mysm1‐shRNA knockdown Ba/F3 cells and in Mysm1‐deficient primary haematopoietic stem and progenitor cells."
34114734
reach
"10 To further validate the overlap in the genomic binding sites of MYSM1 and cMYC in Ba/F3 cells, the binding of cMYC at the MYSM1‐binding sites of select genes encoding RPs and translation factors was tested and confirmed with ChIP‐qPCR (Figure 1E, Supporting Information S1)."
34114734
reach
"The binding of MYSM1 and cMYC at the shared sites was also confirmed in cells derived from EuMyc mouse B cell lymphoma, 14 , 15 specifically for the Rpl7 and Eef1g gene promoters (data not shown)."
34114734
reach
"Overall, we demonstrate shared DNA binding of cMYC and MYSM1 at the promoters of genes encoding ribosomal proteins and translation factors."
34114734
reach
"Meanwhile, co-Immunoprecipitation (co-IP) was performed to detect the interaction between MYSM1 and c-Myc and results showed that MYSM1 protein co-immunoprecipitated with c-Myc in murine RAW264.7 cells."
27590507
ESR1 affects MYSM1
4 | 1 2
MYSM1 binds ESR1. 7 / 7
4 | 1 2
biogrid
No evidence text available
38177530
biogrid
No evidence text available
38177530
biogrid
No evidence text available
38177530
biogrid
No evidence text available
38177530
reach
"MYSM1 physically associates with ERalpha in breast cancer cells."
38177530
sparser
"Virtual screening identified Imatinib as a small molecule capable of interacting with the catalytic MPN domain of MYSM1, and this effectively inhibited breast cancer cell growth, highlighting the MYSM1-ERα axis as a promising target to overcome endocrine independence in breast cancer [ xref ]."
| PMC
sparser
"A virtual screen shows that the small molecule Imatinib could potentially interact with catalytic MPN domain of MYSM1 to inhibit BCa cell growth via MYSM1-ERα axis."
38177530
SMARCA2 affects MYSM1
| 1 5
SMARCA2 binds SMARCA4 and MYSM1. 4 / 4
| 4
sparser
"To further determine the association of MYSM1 and BRM or BRG-1 at the EBF1 promoter locus, sequential two-step ChIP assays were performed. xref shows the association of both MYSM1 and BRM and/or BRG-1 at the Ebf1 promoter locus of WT BM Lin − progenitors."
22169041
sparser
"Collectively, these data demonstrate that MYSM1 interacts with E2A and BRM and/or BRG-1 and its association is likely involved in MYSM1-mediated Ebf1 transcription in B-cell precursors."
22169041
sparser
"An interaction of MYSM1 with the BRM and BRG1 components of the SWI/SNF chromatin remodeling complex was also suggested to have a role in the regulation of Ebf1 locus expression [ xref ]."
32344625
sparser
"To confirm the interaction of MYSM1 with BRM or BRG-1, we performed co-immunoprecipitation assays. xref show that MYSM1 was co-precipitated with endogenous BRM and BRG-1 in 293 cells that were transfected with the Flag-MYSM1 expression vector ( xref )."
22169041
MYSM1 binds SMARCA2. 2 / 2
| 1 1
sparser
"To further determine the association of MYSM1 and BRM or BRG-1 at the EBF1 promoter locus, sequential two-step ChIP assays were performed. xref shows the association of both MYSM1 and BRM and/or BRG-1 at the Ebf1 promoter locus of WT BM Lin − progenitors."
22169041
reach
"Collectively, these data demonstrate that MYSM1 interacts with E2A and BRM and/or BRG-1 and its association is likely involved in MYSM1 mediated Ebf1 transcription in B-cell precursors."
22169041
Mice affects MYSM1
| 6
Mice inhibits MYSM1.
| 3
Mice inhibits MYSM1. 3 / 3
| 3
reach
"Interestingly, the ATP content of serum from Compound C‐ and PFTα‐treated Mysm1 knockdown mice was dramatically decreased compared to that of serum from Mysm1 knockdown mice (Figure 6K)."
36414403
reach
"MYSM1 knockdown mice protect against myocardial I/R injury in vivo."
39897566
reach
"In line with the high ATP level and improved behavioral data, an increased frequency of spontaneous excitatory postsynaptic currents (sEPSCs) in MHb was found in Mysm1 knockdown mice, indicating upregulation of neuronal activities (Figure 5D)."
36414403
Mice activates MYSM1.
| 3
Mice activates MYSM1. 3 / 3
| 3
reach
"The recipient mice were treated either with tamoxifen to induce Mysm1‐deletion or with vehicle corn oil."
34114734
reach
"The recipient mice were bled at 12-weeks to confirm the normal reconstitution with donor bone marrow across the genotypes (data not shown), and subsequently all the mice were administered with tamoxifen to induce the Mysm1 to Mysm1 allele conversion."
36611064
reach
"All mice were treated with tamoxifen to induce the Mysm1 fl -to- Mysm1 Δ allele rearrangement and maintained for 24 weeks prior to the analysis."
27833034
reach
"Notably, STAT1 knockdown (STAT1-KD, Figure S6J) reversed MYSM1-mediated up-regulation of Mlkl and Ripk3 mRNAs (Figure 7J-K), confirming that MYSM1 transcriptionally increased the expression of necroptosis-related genes via STAT1."
39897566
reach
"In summary, these results showed that MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1."
39897566
reach
"MYSM1 initiates the expression of necroptosis-related genes via promoting the transcription factor function of STAT1."
39897566
reach
"Additionally, MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1.Conclusion: This study illustrated a MYSM1-STAT1 axis in regulating myocardial I/R injury and identified MYSM1 as a pharmacological target for myocardial I/R injury."
39897566
reach
"Subsequently, MYSM1 initiated the expression of necroptosis-related genes by promoting the transcriptional activity of STAT1.The JAMMs family represents the sole zinc metalloprotease family, while the remaining DUB families consist of cysteine peptidases 10."
39897566
reach
"In addition, MYSM1 initiates the expression of necroptosis-related genes by promoting the transcription factor function of STAT1."
39897566
MYSM1 affects cellular senescence
| 4 2
MYSM1 inhibits cellular senescence. 6 / 6
| 4 2
eidos
"MYSM1 Represses Senescence in Mice As senescence is characterized by the extensive secretion of pro-inflammatory cytokines , [ ."
33240758
eidos
"MYSM1 represses senescence in aged mice ."
33240758
eidos
"Whilst evaluating the molecular mechanisms underlying the MYSM1-mediated aging process , we revealed that MYSM1 represses cellular senescence and aging process by facilitation of HR DNA repair ."
33240758
reach
"MYSM1 functionally represses cellular senescence and the aging process in human and mice primary cells and in mice organs."
33240758
reach
"Knockdown of MYSM1 promoted cell proliferation and suppressed senescence of CRPC cells under condition of androgen ablation."
31761786
eidos
"MYSM1 functionally represses cellular senescence and the aging process by promoting DNA repair ."
33240758
MYSM1 affects SMARCA2
| 1 5
SMARCA2 binds SMARCA4 and MYSM1. 4 / 4
| 4
sparser
"To further determine the association of MYSM1 and BRM or BRG-1 at the EBF1 promoter locus, sequential two-step ChIP assays were performed. xref shows the association of both MYSM1 and BRM and/or BRG-1 at the Ebf1 promoter locus of WT BM Lin − progenitors."
22169041
sparser
"Collectively, these data demonstrate that MYSM1 interacts with E2A and BRM and/or BRG-1 and its association is likely involved in MYSM1-mediated Ebf1 transcription in B-cell precursors."
22169041
sparser
"An interaction of MYSM1 with the BRM and BRG1 components of the SWI/SNF chromatin remodeling complex was also suggested to have a role in the regulation of Ebf1 locus expression [ xref ]."
32344625
sparser
"To confirm the interaction of MYSM1 with BRM or BRG-1, we performed co-immunoprecipitation assays. xref show that MYSM1 was co-precipitated with endogenous BRM and BRG-1 in 293 cells that were transfected with the Flag-MYSM1 expression vector ( xref )."
22169041
MYSM1 binds SMARCA2. 2 / 2
| 1 1
sparser
"To further determine the association of MYSM1 and BRM or BRG-1 at the EBF1 promoter locus, sequential two-step ChIP assays were performed. xref shows the association of both MYSM1 and BRM and/or BRG-1 at the Ebf1 promoter locus of WT BM Lin − progenitors."
22169041
reach
"Collectively, these data demonstrate that MYSM1 interacts with E2A and BRM and/or BRG-1 and its association is likely involved in MYSM1 mediated Ebf1 transcription in B-cell precursors."
22169041
MYSM1 affects HELLS
3 | 3
MYSM1 binds HELLS. 5 / 5
3 | 2
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
sparser
"However, in melanoma cells, MYSM1 was not found in proximity to either HH3 or Ki-67 in heterochromatin during cell cycle [ xref ], warranting more detailed analyses of the putative function of the MYSM1-HELLS interaction in DDR and gene transcription."
32466590
biogrid
No evidence text available
32466590
sparser
"Therefore, the potential role of the MYSM1-RFC-HELLS interaction in DNA repair and genome integrity will now need to be verified in tumor samples at different tumor stages and in response to treatment."
32466590
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
MYSM1 affects H2AC20
3 1 | 2
MYSM1 binds H2AC20.
3 | 2
MYSM1 binds H2AC20. 5 / 5
3 | 2
biogrid
No evidence text available
21245042
sparser
"In addition, as proof of principle of the co-IP approach, MYSM1 interaction with ubiquitinated histone H2A (H2Aub), a known substrate of the H2A deubiquitinase, was detectable in the anti-Flag pull-down from MYSM1-Flag transfectants, as expected ( xref C, lower panel)."
32466590
sparser
"On the other hand, nuclear MYSM1 was constitutively associated with pCAF and histone H2A but not TRAF3, TRAF6, or K63- or K48-linked polyubiquitins."
26474655
biogrid
No evidence text available
17707232
biogrid
No evidence text available
17707232
MYSM1 deubiquitinates H2AC20.
1 |
MYSM1 deubiquitinates H2AC20. 1 / 1
1 |
ubibrowser
"Myb-Like, SWIRM, and MPN domains 1 (<span class="match term0">MYSM1</span>) is a metalloprotease that deubiquitinates the K119-monoubiquitinated form of <span class="match term1">histone 2A</span> (H2A), a chromatin marker associated with gene transcription silencing."
26220525
MYSM1 affects EBF1
| 4 2
MYSM1 increases the amount of EBF1.
| 4
MYSM1 increases the amount of EBF1. 4 / 4
| 4
reach
"Similarly, in mouse hematopoietic stem or progenitor cells (HSPCs) MYSM1 was shown to promote the expression of Gfi1, Flt3, Id2, Ebf1 and Pax5 genes, important for the normal progression of hematopoiesis, also via H2A-K119ub deubiquitination of their promoters and other regulatory elements ."
36611064
reach
"In early B cells, MYSM1 promotes expression of Ebf1, a critical transcriptional regulator of B-cell differentiation."
38065233
reach
"In an attempt to investigate how MYSM1 activates Ebf1 transcription, we first determined whether MYSM1 deubiquitinates ubH2A at the Ebf1 promoter locus of B-cell precursors."
22169041
reach
"MYSM1 was shown to promote expression of Ebf1, Pax5, and Id2, key transcriptional regulators of B-cell commitment and differentiation."
38065233
MYSM1 binds EBF1.
| 2
EBF1 binds MYSM1. 2 / 2
| 2
sparser
"These data further demonstrate the direct association of MYSM1 with the Ebf1 promoter region in B-cells and their precursors."
22169041
sparser
"To probe the possible interaction of endogenous MYSM1 and the transcription factors of Ebf1 , we performed co-immunoprecipitation assays."
22169041
ID4 affects MYSM1
| 4 2
ID4 inhibits MYSM1.
| 2
ID4 inhibits MYSM1. 2 / 2
| 2
reach
"Overexpression of Id4 partially restores the function of Mysm1-deficient NSCs."
38342917
reach
"These results revealed that exogenous expression of Id4 rescues some characteristics and functions in Mysm1-deficient NSCs."
38342917
ID4 binds MYSM1.
| 2
ID4 binds MYSM1. 2 / 2
| 2
sparser
"Considering that the expression of MYSM1 increased with age and that Mysm1-deficient NSCs prompt neuron differentiation, suppressing the MYSM1-Id4 axis maybe an ideal strategy for stimulating NSC proliferation and neuron development."
39684760
sparser
"These findings suggest that the MYSM1-Id4 axis represents a potential therapeutic target for regulating NSC proliferation and differentiation."
39684760
ID4 activates MYSM1.
| 2
ID4 activates MYSM1. 2 / 2
| 2
reach
"Thus, we speculate that the decrease in Id4 caused by knocking down Mysm1 may account for the overactivation and decreased glial formation of NSCs.The regulation of stem cell fate is highly complex."
38342917
reach
"Overexpression of Id4 could reverse the hyperproliferation and imbalance differentiation of Mysm1-deficient NSCs."
38342917
HELLS affects MYSM1
3 | 3
MYSM1 binds HELLS. 5 / 5
3 | 2
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
sparser
"However, in melanoma cells, MYSM1 was not found in proximity to either HH3 or Ki-67 in heterochromatin during cell cycle [ xref ], warranting more detailed analyses of the putative function of the MYSM1-HELLS interaction in DDR and gene transcription."
32466590
biogrid
No evidence text available
32466590
sparser
"Therefore, the potential role of the MYSM1-RFC-HELLS interaction in DNA repair and genome integrity will now need to be verified in tumor samples at different tumor stages and in response to treatment."
32466590
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
ZDHHC2 affects MYSM1
| 5
MYSM1 binds ZDHHC2. 5 / 5
| 5
sparser
"Next we purified ubiquitin conjugates from L18-MDP-stimulated Mysm1 − /− BMDMs by TUBE and incubated them with recombinant MYSM1 (Rec-MYSM1) and compared the ubiquitin restriction pattern of Ub-RIP2 with that of AMSH, UCHL3, OTULIN or a combination of these three DUBs (Fig.  xref )."
30405132
sparser
"In further support of these findings, incubation of Ub-RIP2 immunoprecipitated from Mysm1 −/− BMDMs with Rec-MYSM1 resulted in diminished K63-, K27-, and M1-linked, but not K48-linked, ubiquitin chains (Supplementary Fig.  xref )."
30405132
sparser
"In contrast, Rec-MYSM1 had no effect on ubiquitin chains attached to NEMO (Supplementary Fig.  xref ), indicating that MYSM1 was selectively cleaving K63, K27, and M1, but not K48 linkages, from RIP2."
30405132
sparser
"To gain a complete overview of the ubiquitin linkage specificity of MYSM1, we incubated Rec-MYSM1 with Di-ubiqutins (Ub 2 ) of different linkages."
30405132
sparser
"Moreover, in vitro, Rec-MYSM1 could remove polyubiquitins from RIP2, but not NEMO (Supplementary Figure  xref )."
30405132
MYSM1 affects propidium iodide
| 5
MYSM1 increases the amount of propidium iodide.
| 4
MYSM1 increases the amount of propidium iodide. 4 / 4
| 4
reach
"Additionally, MYSM1 knockdown reduced the quantity of Propidium Iodide (PI)-positive nuclei during H/R injury (Figure 4C-D)."
39897566
reach
"We further found that upregulation of MYSM1 increased the quantity of PI-positive nuclei following H/R injury (Figure 4G-H)."
39897566
reach
"Conversely, overexpression of MYSM1 led to a reduction in cell viability (Fig. 4E), an increase in LDH release (Fig. 4F), and a higher quantity of PI-positive nuclei after DOX treatment (Fig. 4G-H)."
39695708
reach
"Additionally, MYSM1 knockdown reduced the quantity of Propidium Iodide (PI)-positive nuclei during H/R injury (Figure 4C-D)."
39897566
MYSM1 activates propidium iodide.
| 1
reach
"Furthermore, MYSM1 silencing decreased PI-positive nuclei in H/R injury (Figure 4K-L)."
39897566
reach
"Thus, MYSM1 is a key inhibitor of innate immunity and autoimmunity and may represent a therapeutic agent in the treatment of infectious, inflammatory, and autoimmune diseases.Initial experiments evalu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
reach
"Thus, MYSM1 is a key suppressor of innate immunity and autoimmunity and represents a potential therapeutic agent for infectious, inflammatory, and autoimmune diseases."
33086059
reach
"Studies have found that MYSM1 was a key inhibitor of innate immunity and autoimmunity."
34670246
eidos
"MYSM1 Represses Innate Immunity and Autoimmunity through Suppressing the cGAS-STING Pathway ."
33086059
reach
"The level of IFN-β , IL-6, and Cxcl10 mRNAs ( Figure 2 G), the expression of IFN-β, IL-6, and TNF-α proteins ( Figure 2 H), and the phosphorylation of TBK1 and IRF3 proteins ( Figure 2 I) induced by H[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects aging process
| 5
MYSM1 inhibits aging process. 5 / 5
| 5
eidos
"MYSM1 functionally represses cellular senescence and the aging process by promoting DNA repair ."
33240758
eidos
"Functionally , MYSM1 functionally represses DDR-associated SASP and the aging process ."
33240758
eidos
"Mechanistically , MYSM1 represses the aging process by promoting homologous recombination ( HR ) mediated DNA repair ."
33240758
eidos
"Whilst evaluating the molecular mechanisms underlying the MYSM1-mediated aging process , we revealed that MYSM1 represses cellular senescence and aging process by facilitation of HR DNA repair ."
33240758
eidos
"MYSM1 mechanistically attenuates the aging process by promoting DNA repair processes ."
33240758
MYSM1 affects ZDHHC2
| 5
MYSM1 binds ZDHHC2. 5 / 5
| 5
sparser
"Next we purified ubiquitin conjugates from L18-MDP-stimulated Mysm1 − /− BMDMs by TUBE and incubated them with recombinant MYSM1 (Rec-MYSM1) and compared the ubiquitin restriction pattern of Ub-RIP2 with that of AMSH, UCHL3, OTULIN or a combination of these three DUBs (Fig.  xref )."
30405132
sparser
"In further support of these findings, incubation of Ub-RIP2 immunoprecipitated from Mysm1 −/− BMDMs with Rec-MYSM1 resulted in diminished K63-, K27-, and M1-linked, but not K48-linked, ubiquitin chains (Supplementary Fig.  xref )."
30405132
sparser
"In contrast, Rec-MYSM1 had no effect on ubiquitin chains attached to NEMO (Supplementary Fig.  xref ), indicating that MYSM1 was selectively cleaving K63, K27, and M1, but not K48 linkages, from RIP2."
30405132
sparser
"To gain a complete overview of the ubiquitin linkage specificity of MYSM1, we incubated Rec-MYSM1 with Di-ubiqutins (Ub 2 ) of different linkages."
30405132
sparser
"Moreover, in vitro, Rec-MYSM1 could remove polyubiquitins from RIP2, but not NEMO (Supplementary Figure  xref )."
30405132
MYSM1 affects Syndrome
| 5
MYSM1 activates Syndrome.
| 3
MYSM1 activates Syndrome. 3 / 3
| 3
reach
"Homozygous LOF mutations in MYSM1 cause a syndrome of progressive bone marrow failure, immunodeficiency, and developmental abnormalities."
29731008
reach
"Following molecular diagnosis, the patient was prepared for HSCT.To date, only nine patients with IBMF syndrome caused by MYSM1 variation have been reported, including six patients homozygous for E390[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32640305
reach
"Recently, biallelic variants in MYSM1 have been reported to lead to a rare bone marrow failure (BMF) syndrome (OMIM #618116) in humans, which has been observed in only nine patients ( Al-Herz et al., [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32640305
MYSM1 inhibits Syndrome.
| 2
MYSM1 inhibits Syndrome. 2 / 2
| 2
reach
"MYSM1 deficiency causes inherited bone marrow failure syndrome (IBMFS)."
39233474
reach
"Indeed, the latest evidence suggests that human cord blood HSCs are more susceptible to ferroptosis than downstream progenitor cells ex vivo due to a low level of antioxidant protein synthesis, and MY[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38582294
MYSM1 affects MAPK cascade
| 5
MYSM1 inhibits MAPK cascade. 5 / 5
| 5
reach
"MYSM1 attenuates NF-kappaB and MAPK signaling pathways in chondrocytes."
39746943
reach
"These results indicate that MYSM1 deactivates NF-κB and MAPK signaling pathways, thereby protecting mouse chondrocytes against inflammation."
39746943
reach
"MYSM1 deletion activated NF-κB and MAPK signaling, significantly promoting OA development and exacerbating cartilage degeneration."
39746943
reach
"Mechanistically, MYSM1 inhibited the NF-kappaB and MAPK signaling pathways."
39746943
reach
"Our findings demonstrate that Mysm1 overexpression deactivates NF-κB and MAPK signaling pathways, whereas Mysm1 knockdown significantly activates these pathways in mouse chondrocytes."
39746943
MYSM1 affects LDH
| 5
MYSM1 increases the amount of LDH.
| 3
MYSM1 increases the amount of LDH. 3 / 3
| 3
reach
"As shown in Figure 4I-J, MYSM1 silencing markedly increased cell viability (Figure 4I) and suppressed the LDH release (Figure 4J) during H/R injury."
39897566
reach
"MYSM1 overexpression markedly decreased cell viability (Fig. 7O) and promoted the LDH release (Fig. 7P) in DOX-treated HL-1 cells, while the ferroptosis inhibitor Fer-1 significantly inhibited these effects (Fig. 7O-P)."
39695708
reach
"To determine the association of JAMMs family genes with myocardial I/R injury, we overexpressed JAMMs family in HL-1 cells (Figure S1A) and observed that MYSM1 overexpression resulted in decreased cell viability (Figure 1A) and increased LDH release (Figure 1B) under H/R treatment."
39897566
MYSM1 inhibits LDH.
| 1
MYSM1 inhibits LDH. 1 / 1
| 1
reach
"Notably, MYSM1 knockdown significantly enhanced cell viability (Figure 4A) and inhibited the activity of LDH in the cellular supernatant (Figure 4B) under H/R injury."
39897566
MYSM1 decreases the amount of LDH.
| 1
MYSM1 decreases the amount of LDH. 1 / 1
| 1
reach
"We found that MYSM1 knockdown dramatically increased cell viability (Fig. 4A) and suppressed the LDH release (Fig. 4B) after DOX treatment."
39695708
MYSM1 affects ID4
| 3 2
MYSM1 increases the amount of ID4.
| 3
MYSM1 increases the amount of ID4. 3 / 3
| 3
reach
"Collectively, these data revealed that Mysm1 orchestrates histone modifications at the Id4 promoter region and activates Id4 transcription."
38342917
reach
"In NSCs, we have observed a similar situation, indicating that Mysm1 orchestrates histone modifications and activates Id4 transcription."
38342917
reach
"Mechanistic investigations with RNA sequencing and genome-wide CUT&Tag analysis revealed that Mysm1 epigenetically regulated Id4 transcription by regulating histone modification at the promoter region."
38342917
MYSM1 binds ID4.
| 2
ID4 binds MYSM1. 2 / 2
| 2
sparser
"Considering that the expression of MYSM1 increased with age and that Mysm1-deficient NSCs prompt neuron differentiation, suppressing the MYSM1-Id4 axis maybe an ideal strategy for stimulating NSC proliferation and neuron development."
39684760
sparser
"These findings suggest that the MYSM1-Id4 axis represents a potential therapeutic target for regulating NSC proliferation and differentiation."
39684760
MYSM1 affects DNA Damage
| 5
MYSM1 inhibits DNA Damage.
| 3
MYSM1 inhibits DNA Damage. 3 / 3
| 3
reach
"Mechanistically, defective development of Mysm1-deficient mice correlated with altered gene transcription, increased apoptosis, and increased DNA damage in a variety of tissues, and was largely rescued by concomitant deletion of tumor suppressor p53 [38,42,43,44]."
32466590
reach
"MYSM1 attenuates DNA damage signals triggered by physiologic and genotoxic DNA breaks."
38065233
reach
"Loss of MYSM1 results in increased DNA damage signaling in lymphocytes and nonlymphoid cells, but its function in cellular responses to DNA injury has not been characterized."
38065233
MYSM1 activates DNA Damage.
| 2
MYSM1 activates DNA Damage. 2 / 2
| 2
reach
"MYSM1 deficiency spontaneously perturbs the proper repair of DNA damage to accumulate DNA double strand breaks (DSB), accompanied by cellular senescence (Kroeger et al, 2020; Nishi et al, 2014; Tian et al, 2020a)."
38177530
reach
"Therefore, we investigated whether MYSM1 mediates DOX-induced DNA damage in cardiomyocytes by detecting 𝛾 -H2AX."
39695708
H2AC20 affects MYSM1
3 | 2
MYSM1 binds H2AC20. 5 / 5
3 | 2
biogrid
No evidence text available
21245042
sparser
"In addition, as proof of principle of the co-IP approach, MYSM1 interaction with ubiquitinated histone H2A (H2Aub), a known substrate of the H2A deubiquitinase, was detectable in the anti-Flag pull-down from MYSM1-Flag transfectants, as expected ( xref C, lower panel)."
32466590
sparser
"On the other hand, nuclear MYSM1 was constitutively associated with pCAF and histone H2A but not TRAF3, TRAF6, or K63- or K48-linked polyubiquitins."
26474655
biogrid
No evidence text available
17707232
biogrid
No evidence text available
17707232
SWIRM domain affects MYSM1
| 4
MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
| 2
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
MYSM1 binds STAT1 and SWIRM domain. 2 / 2
| 2
reach
"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
39897566
reach
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
SMARCA4 affects SMARCA2
| 4
SMARCA2 binds SMARCA4 and MYSM1. 4 / 4
| 4
sparser
"To further determine the association of MYSM1 and BRM or BRG-1 at the EBF1 promoter locus, sequential two-step ChIP assays were performed. xref shows the association of both MYSM1 and BRM and/or BRG-1 at the Ebf1 promoter locus of WT BM Lin − progenitors."
22169041
sparser
"Collectively, these data demonstrate that MYSM1 interacts with E2A and BRM and/or BRG-1 and its association is likely involved in MYSM1-mediated Ebf1 transcription in B-cell precursors."
22169041
sparser
"An interaction of MYSM1 with the BRM and BRG1 components of the SWI/SNF chromatin remodeling complex was also suggested to have a role in the regulation of Ebf1 locus expression [ xref ]."
32344625
sparser
"To confirm the interaction of MYSM1 with BRM or BRG-1, we performed co-immunoprecipitation assays. xref show that MYSM1 was co-precipitated with endogenous BRM and BRG-1 in 293 cells that were transfected with the Flag-MYSM1 expression vector ( xref )."
22169041
RFC5 affects MYSM1
3 | 1
MYSM1 binds RFC5. 3 / 3
3 |
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
MYSM1 affects cGAS-STING
| 4
MYSM1 inhibits cGAS-STING.
| 2
MYSM1 bound to STING1 inhibits cGAS-STING. 1 / 1
| 1
reach
"MYSM1 binds to STING and inhibits the cGAS-STING signaling pathway [169]."
36359370
MYSM1 inhibits cGAS-STING. 1 / 1
| 1
reach
"These results indicate that MYSM1 inhibits the cGAS-STING pathway by interacting with the STING N terminus via the SWIRM domain.Since MYSM1 is a deubiquitinase, the role of deubiquitinase in the suppr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 activates cGAS-STING.
| 2
MYSM1 bound to STING1 activates cGAS-STING. 1 / 1
| 1
reach
"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 bound to K63 and STING1 activates cGAS-STING. 1 / 1
| 1
reach
"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects SWIRM domain
| 4
MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
| 2
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
MYSM1 binds STAT1 and SWIRM domain. 2 / 2
| 2
reach
"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
39897566
reach
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
MYSM1 affects SMARCA4
| 4
SMARCA2 binds SMARCA4 and MYSM1. 4 / 4
| 4
sparser
"To further determine the association of MYSM1 and BRM or BRG-1 at the EBF1 promoter locus, sequential two-step ChIP assays were performed. xref shows the association of both MYSM1 and BRM and/or BRG-1 at the Ebf1 promoter locus of WT BM Lin − progenitors."
22169041
sparser
"Collectively, these data demonstrate that MYSM1 interacts with E2A and BRM and/or BRG-1 and its association is likely involved in MYSM1-mediated Ebf1 transcription in B-cell precursors."
22169041
sparser
"An interaction of MYSM1 with the BRM and BRG1 components of the SWI/SNF chromatin remodeling complex was also suggested to have a role in the regulation of Ebf1 locus expression [ xref ]."
32344625
sparser
"To confirm the interaction of MYSM1 with BRM or BRG-1, we performed co-immunoprecipitation assays. xref show that MYSM1 was co-precipitated with endogenous BRM and BRG-1 in 293 cells that were transfected with the Flag-MYSM1 expression vector ( xref )."
22169041
MYSM1 affects RFC5
3 | 1
MYSM1 binds RFC5. 3 / 3
3 |
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
MYSM1 affects PARP1
1 | 2 1
MYSM1 deubiquitinates PARP1.
| 2
MYSM1 deubiquitinates PARP1. 2 / 2
| 2
reach
"MYSM1 deubiquitinates and stabilizes PARP1 in an MPN domain-dependent manner."
39907013
reach
"MYSM1 Mediates Cardiac Parthanatos and Hypertrophy by Deubiquitinating PARP1."
39907013
MYSM1 binds PARP1.
1 | 1
PARP1 binds MYSM1. 2 / 2
1 | 1
sparser
"This study identified the role of the MYSM1-PARP1 axis in mediating cardiac hypertrophy and suggested that MYSM1 is a promising pharmacological target for the treatment of cardiac hypertrophy."
39907013
biogrid
No evidence text available
34079125
MYSM1 affects Mice
| 4
MYSM1 inhibits Mice. 4 / 4
| 4
reach
"Mice with Mysm1 knockdown by crossing Mysm1 floxed mice with Nestin-Cre mice exhibited abnormal brain development with microcephaly."
38342917
reach
"Interestingly, type I IFN, pro-inflammatory cytokines, TBK1, and IRF3 induced by DNA sensing were downregulated by MYSM1, upregulated by sh- Mysm1 , and enhanced in Mysm1 −/− mice."
33086059
reach
"Increased MYSM1 can decrease type I IFN, IL-6, and other inflammatory cytokines in SLE mice (86)."
35603197
reach
"Moreover, cGAMP induced the upregulation of IFN-β , IL-6 , and Cxcl10 mRNAs ( Figure 3 C), as well as IFN-β, IL-6, and TNF-α proteins ( Figure 3 D), and was significantly higher in BMDMs from Mysm1 −/[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects MYSM1
| 4
MYSM1 activates MYSM1.
| 2
MYSM1 activates MYSM1. 2 / 2
| 2
reach
"XREF_FIG shows that forced expression of MYSM1, but not control, rescued the enhanced plasma cell differentiation of Mysm1 -/- B cells."
26348977
reach
"Loss of Mysm1 Causes Skin Atrophy and Reduced Skin Cellularity in Mysm1 Deficient Mice."
29495602
MYSM1 dephosphorylates MYSM1.
| 1
MYSM1 dephosphorylates MYSM1. 1 / 1
| 1
reach
"Dephosphorylated and deubiquitinated RIPK2 downstream of MYSM1 maintains articular cartilage homeostasis by antagonizing NF-κB and MAPK signaling in OA.As a deubiquitinase, MYSM1 may not directly dephosphorylate RIPK2; instead, it likely recruits other phosphatases during inflammation."
39746943
MYSM1 decreases the amount of MYSM1.
| 1
MYSM1 decreases the amount of MYSM1. 1 / 1
| 1
reach
"Western blot analysis showed that MYSM1 siRNA significantly reduced the expression of MYSM1 protein compared with siRNA non transfected or control siRNA transfected SW480 cells (XREF_FIG)."
28498834
MYSM1 affects Interferon
| 4
MYSM1 inhibits Interferon.
| 2
MYSM1 inhibits Interferon. 2 / 2
| 2
reach
"Increased MYSM1 can decrease type I IFN, IL-6, and other inflammatory cytokines in SLE mice (86)."
35603197
reach
"Interestingly, type I IFN, pro-inflammatory cytokines, TBK1, and IRF3 induced by DNA sensing were downregulated by MYSM1, upregulated by sh- Mysm1 , and enhanced in Mysm1 −/− mice."
33086059
MYSM1 activates Interferon.
| 2
MYSM1 activates Interferon. 2 / 2
| 2
reach
"Similarly, upon infection with L. monocytogenes, Mysm1 −/− mice elicited a more robust pro-inflammatory and type I IFN gene activation in multiple organs including the peritoneum ( Figure S7 B) and sp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26474655
reach
"Interestingly, type I IFN, pro-inflammatory cytokines, TBK1, and IRF3 induced by DNA sensing were downregulated by MYSM1, upregulated by sh- Mysm1 , and enhanced in Mysm1 −/− mice."
33086059
MYSM1 affects IRF8
| 3 1
MYSM1 activates IRF8.
| 2
MYSM1 activates IRF8. 2 / 2
| 2
reach
"We found that IRF2 and IRF8, which are important for HSC homeostasis and commitment as transcription repressors, were expressed at lower levels in Mysm1 -/- HSCs, and Mysm1 enhanced function of the IRF2 and IRF8 promoters, suggesting that Mysm1 governs the IRFs for HSC homeostasis."
27277682
reach
"The results showed that Mysm1 significantly enhances IRF2 and IRF8 promoter activity (-600 to +50) (XREF_SUPPLEMENTARY)."
27277682
MYSM1 decreases the amount of IRF8.
| 1
MYSM1 decreases the amount of IRF8. 1 / 1
| 1
reach
"Mysm1 deficiency reduces IRF2 and IRF8 expression in HSCs."
27277682
MYSM1 binds IRF8.
| 1
IRF2 binds IRF8 and MYSM1. 1 / 1
| 1
sparser
"We then tested Mysm1 binding to the promoter regions of IRF2 and IRF8 by analyzing WT (not Mysm1 −/− ) Lin − BM cells via a ChIP assay after in vitro stimulation with Poly(I:C) or IFN-γ."
27277682
MYSM1 affects EP300
1 | 1 2
MYSM1 binds EP300.
1 | 2
MYSM1 binds EP300 and CREBBP. 2 / 2
| 2
sparser
"2A-DUB associates with histone acetyltransferase p300/CBP-associated factor (PCAF/KAT2B) in a coregulatory protein complex and regulates transcription by coordinating histone acetylating, deubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18929679
sparser
"2A-DUB associates with the histone acetyltransferase p300/CBP-associated factor (p/CAF), a complex that mediates acetylation of histones H3 and H4 in transcriptionally active chromatin ( xref )."
19489724
EP300 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
38177530
MYSM1 activates EP300.
| 1
MYSM1 activates EP300. 1 / 1
| 1
reach
"MYSM1 depletion decreased the recruitment of ERα, pCAF, CBP, and p300 on EREs."
38177530
MYSM1 affects DDR
| 4
MYSM1 inhibits DDR.
| 2
MYSM1 inhibits DDR. 2 / 2
| 2
reach
"Loss of MYSM1 results in persistent downstream DDR signaling."
38065233
reach
"Rather, loss of MYSM1 resulted in persistent DNA damage foci and prolonged DDR signaling."
38065233
MYSM1 activates DDR.
| 2
MYSM1 activates DDR. 2 / 2
| 2
reach
"Increased MYSM1 accelerates DDR resolution."
38065233
reach
"Further investigations are needed to characterize factors that modulate MYSM1 activity in DNA damage.Loss of MYSM1 results in persistent 53BP1 foci and prolonged DDR signaling in pre–B cells undergoing Igl recombination."
38065233
MYSM1 affects CREBBP
1 | 1 2
MYSM1 binds CREBBP.
1 | 2
MYSM1 binds EP300 and CREBBP. 2 / 2
| 2
sparser
"2A-DUB associates with histone acetyltransferase p300/CBP-associated factor (PCAF/KAT2B) in a coregulatory protein complex and regulates transcription by coordinating histone acetylating, deubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18929679
sparser
"2A-DUB associates with the histone acetyltransferase p300/CBP-associated factor (p/CAF), a complex that mediates acetylation of histones H3 and H4 in transcriptionally active chromatin ( xref )."
19489724
CREBBP binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
38177530
MYSM1 activates CREBBP.
| 1
MYSM1 activates CREBBP. 1 / 1
| 1
reach
"MYSM1 depletion decreased the recruitment of ERα, pCAF, CBP, and p300 on EREs."
38177530
Histone affects MYSM1
| 4
MYSM1 binds Histone. 4 / 4
| 4
sparser
"The SWIRM domain of MYSM1, in contrast, does not have direct DNA binding activity [ xref ], and although it is a common domain-type for chromatin-associated proteins [ xref , xref ], it was shown to be dispensable for MYSM1 interactions with histones, at least in some systems [ xref ]."
32344625
sparser
"The SANT domain of MYSM1 binds to DNA and is crucial for MYSM1’s binding to histones [ xref ]."
39695708
sparser
"These data suggest that MYSM1 interacts with other histone modifiers to regulate chromatin status at the Ebf1 locus."
22169041
sparser
"The SANT domain of MYSM1 can bind to DNA and is required for MYSM1 to bind to histones xref , xref ."
39897566
ESR affects MYSM1
| 4
ESR inhibits MYSM1.
| 2
ESR inhibits MYSM1. 2 / 2
| 2
reach
"When cycloheximide (CHX) was applied at specified time points to inhibit protein synthesis, depletion of MYSM1 significantly accelerated ERα degradation and ectopic expression of wild-type MYSM1 plasmid (MYSM1-FL) ameliorated ERα degradation process (Fig. 3C,E)."
38177530
reach
"Compared with the full length of MYSM1, the lack of SWIRM domain had little effect on ERα-induced transactivation, while MYSM1-ΔSANT or MYSM1-ΔMPN dramatically reduced ERα action (Fig. 2C), indicating that the SANT or MPN domain is required for the co-activation function of MYSM1 on ERα-mediated transactivation.Indeed, ERα behaves in numerous biological processes through its target genes."
38177530
ESR binds MYSM1.
| 2
MYSM1 binds ESR. 2 / 2
| 2
reach
"Then, we set out to examine the interaction between MYSM1 and ERα in mammalian cells by Co-immunoprecipitation (Co-IP)."
38177530
reach
"The precipitation results demonstrated that loss of SANT (Swi3, Ada2, N-CoR, (TF) IIIB) domain more obviously impaired the association between MYSM1 and ERα, compared with that of SWIRM or MPN domain (Figs."
38177530
EBF1 affects MYSM1
| 2 2
EBF1 increases the amount of MYSM1.
| 2
EBF1 increases the amount of MYSM1. 2 / 2
| 2
reach
"We identified the transcription factor EBF1 as a pivotal target gene of MYSM1 in lymphoid precursors and demonstrated that MYSM1 epigenetically de-represses EBF1 transcription by orchestrating histone modifications and regulating recruitment of the transcription factors to the EBF1 locus."
22169041
reach
"In an attempt to investigate how MYSM1 activates Ebf1 transcription, we first determined whether MYSM1 deubiquitinates ubH2A at the Ebf1 promoter locus of B-cell precursors."
22169041
EBF1 binds MYSM1.
| 2
EBF1 binds MYSM1. 2 / 2
| 2
sparser
"These data further demonstrate the direct association of MYSM1 with the Ebf1 promoter region in B-cells and their precursors."
22169041
sparser
"To probe the possible interaction of endogenous MYSM1 and the transcription factors of Ebf1 , we performed co-immunoprecipitation assays."
22169041
BBC3 affects MYSM1
| 2 2
BBC3 activates MYSM1.
| 2 1
BBC3 activates MYSM1. 3 / 3
| 2 1
sparser
"The downregulation of cell-cycle progression genes, together with the upregulation of p53 effectors in Mysm1 −/− Puma −/− MPPs further supports persistent stress-response activation in these cells."
26768662
reach
"Considering that Mysm1 -/- p53 -/- mice displayed complete rescue of hematopoietic dysfunction, 38 the other p53 effectors identified in our Mysm1 -/- Puma -/- transcriptome analysis may contribute to the PUMA independent but p53 dependent Mysm1 -/- phenotypes."
26768662
reach
"XREF_BIBR, XREF_BIBR PUMA drives MPP cell depletion in Mysm1 deficiency."
26768662
BBC3 binds MYSM1.
| 1
MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
| 1
sparser
"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
26768662
MiR-129-5p affects MYSM1
| 3
MiR-129-5p decreases the amount of MYSM1.
| 2
MiR-129-5p decreases the amount of MYSM1. 2 / 2
| 2
reach
"When overexpressed in astrocytes, miR-129-5p significantly suppressed Mysm1 expression, promoted phosphorylation of p53 and AMPK, and enhanced the expression of PGC1alpha, factors previously associated with ATP production."
40344568
reach
"Interestingly, miR-129-5p overexpression also effectively decreased Mysm1 levels in depressive mice.In addition, Chang et al. identified MAPK1 as a direct target of miR-129-5p [58]."
40344568
MiR-129-5p binds MYSM1.
| 1
MiR-129-5p binds MYSM1. 1 / 1
| 1
reach
"We further utilized a luciferase reporter assay to validate the direct interaction between miR-129-5p and Mysm1."
40344568
Hsa-miR-8485 affects MYSM1
3 |
Hsa-miR-8485 decreases the amount of MYSM1. 3 / 3
3 |
biopax:mirtarbase
No evidence text available
23313552
biopax:mirtarbase
No evidence text available
27418678
biopax:mirtarbase
No evidence text available
23824327
Bisphenol A affects MYSM1
3 |
Bisphenol A decreases the amount of MYSM1. 3 / 3
3 |
ctd
No evidence text available
32156529
ctd
No evidence text available
30816183
ctd
No evidence text available
25181051
Benzo[a]pyrene affects MYSM1
3 |
Benzo[a]pyrene decreases the amount of MYSM1.
2 |
Benzo[a]pyrene decreases the amount of MYSM1. 2 / 2
2 |
ctd
No evidence text available
19770486
ctd
No evidence text available
21839799
Benzo[a]pyrene increases the amount of MYSM1.
1 |
Benzo[a]pyrene increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
22228805
TRAF3 affects MYSM1
| 1 2
MYSM1 binds TRAF3. 3 / 3
| 1 2
sparser
"Notably, TRAF2 and TRAF3—key adapters in NF-κB signaling and known to form functional complexes—were among the top hits, consistent with prior reports of MYSM1–TRAF3 or MYSM1–TRAF6 interactions xref – xref ."
41162619
sparser
"Reciprocal knockdowns demonstrated that TRAF2 depletion diminished MYSM1–TRAF3 binding, and TRAF3 depletion diminished MYSM1–TRAF2 binding (Fig. xref ), suggesting that MYSM1 engages each protein in a non–mutually exclusive manner."
41162619
reach
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
TERF1 affects MYSM1
| 3
TERF1 binds MYSM1. 3 / 3
| 3
sparser
"Gel retardation analysis with a series of mutated telomere probes revealed that the internal GGGTTT sequence in the two-telomere repeats is critical for binding of Myb-like domain of RTBP1, which is consistent with the model of the TRF1."
10811811
sparser
"TRF1 is unique in that the two Myb-like domains of the TRF1 homodimer bind to two AGGGTT sites independently and with extreme flexibility in terms of the spatial arrangement of the two target sites ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15904916
sparser
"It was suggested that in vitro the Myb-like binding domain of TRF1 binds to DNA essentially independently of the rest of the protein (König et al. xref ; Bianchi et al. xref ; Court et al. xref )."
37118559
RIPK2 affects NOD2
| 3
RIPK2 binds NOD2 and MYSM1. 2 / 2
| 2
sparser
"To explore further, we assessed by immunoprecipitation the interaction of MYSM1 with the other components in the NOD2:RIP2 complex."
30405132
sparser
"Importantly, LCL-161 did not abolish the enhanced MDP-induced NOD2:RIP2 complex assembly or phosphorylation of RIP2 and IKKα/β caused by MYSM1 deficiency (Supplementary Figure  xref )."
30405132
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
RAP1 affects MYSM1
| 3
RAP1 binds MYSM1. 3 / 3
| 3
sparser
"Based on the available crystal structures, with such a short DNA duplex it is difficult to rationalize how Rap1 DBD would be able to bind with both Myb-like domains interacting with the DNA."
25637888
sparser
"The double-stranded part of the telomere is bound by Rap1 (repressor/activator-site binding protein) through its Myb-like domain, which in turn causes nucleation of a protein complex by recruiting, th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17954006
sparser
"The transient opening of the wrapping loop destabilizes this clamped structure and allows Rap1 binding to DNA through a single Myb-like domain [ xref , xref ]."
36291091
PU.1 affects MYSM1
| 3
MYSM1 binds PU.1. 3 / 3
| 3
sparser
"Through the sequential two-step ChIP assays, we found that MYSM1 was associated with PU.1 at the Pax5 locus in naïve WT B cells ( xref )."
26348977
sparser
"Mechanistically, Mysm1 interacts with Pu.1 to co-occupy the Flt3 promoter and induce Flt3 expression."
33126080
sparser
"Consistent with earlier observations ( xref ), the association of MYSM1 and PU.1 with the Pax5 locus was not detected in WT plasma cells."
26348977
PRSS27 affects MYSM1
| 1 2
PRSS27 binds MYSM1.
| 2
MYSM1 binds PRSS27. 2 / 2
| 2
sparser
"Virtual screening identified Imatinib as a small molecule capable of interacting with the catalytic MPN domain of MYSM1, and this effectively inhibited breast cancer cell growth, highlighting the MYSM1-ERα axis as a promising target to overcome endocrine independence in breast cancer [ xref ]."
| PMC
sparser
"A virtual screen shows that the small molecule Imatinib could potentially interact with catalytic MPN domain of MYSM1 to inhibit BCa cell growth via MYSM1-ERα axis."
38177530
PRSS27 inhibits MYSM1.
| 1
PRSS27 inhibits MYSM1. 1 / 1
| 1
reach
"Compared with the full length of MYSM1, the lack of SWIRM domain had little effect on ERα-induced transactivation, while MYSM1-ΔSANT or MYSM1-ΔMPN dramatically reduced ERα action (Fig. 2C), indicating that the SANT or MPN domain is required for the co-activation function of MYSM1 on ERα-mediated transactivation.Indeed, ERα behaves in numerous biological processes through its target genes."
38177530
PCNA affects MYSM1
2 | 1
MYSM1 binds PCNA. 2 / 2
2 |
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
NOD2 affects RIPK2
| 3
RIPK2 binds NOD2 and MYSM1. 2 / 2
| 2
sparser
"To explore further, we assessed by immunoprecipitation the interaction of MYSM1 with the other components in the NOD2:RIP2 complex."
30405132
sparser
"Importantly, LCL-161 did not abolish the enhanced MDP-induced NOD2:RIP2 complex assembly or phosphorylation of RIP2 and IKKα/β caused by MYSM1 deficiency (Supplementary Figure  xref )."
30405132
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 affects polyubiquitin chains
| 3
MYSM1 activates polyubiquitin chains. 3 / 3
| 3
reach
"Mechanistically, MYSM1 promotes the stability of STAT1 by cleaving polyubiquitin chains from the K379 residue of STAT1."
39897566
reach
"Notably, UCHL1, OTUB1, OTUB2, OTUD5, USP25, MYSM1, and MCPIP1 (Figure 3A) negatively regulate virus-induced IFN-I production and antiviral activities by cleaving K63-linked or other types of polyubiquitin chains from TRAF3 or TRAF6."
34707613
reach
"Taken together, these results suggested that MYSM1 promotes STAT1 stability by cleaving the polyubiquitin chains on K379 residue of STAT1 (Figure 6M)."
39897566
MYSM1 affects miR-200
| 3
MYSM1 inhibits miR-200.
| 2
MYSM1 inhibits miR-200. 2 / 2
| 2
reach
"MYSM1 inhibited the CRC progression via miR-200/CDH1 and inhibition of PI3K/AKT axis [35]."
37580737
reach
"MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling."
34706761
MYSM1 increases the amount of miR-200.
| 1
MYSM1 increases the amount of miR-200. 1 / 1
| 1
reach
"Mechanistically, MYSM1 directly bound to the promoter region of miR-200/CDH1, impaired the enrichment of repressive H2AK119ub1 modification and epigenetically enhanced miR-200/CDH1 expression."
34706761
MYSM1 affects lifespan
| 3
MYSM1 activates lifespan. 3 / 3
| 3
eidos
"Mysm1 deficiency promotes aging and aging-related pathologies and reduces lifespan in mice ."
33240758
eidos
"Remarkably , MYSM1 deficiency facilitates the aging process and reduces lifespan , whereas MYSM1 over-expression attenuates the aging process and increases lifespan in mice ."
33240758
eidos
"MYSM1 deficiency facilitates the aging process and reduces lifespan , whereas MYSM1 over-expression attenuates aging and increases the lifespan of mice ( Figure 7 ) ."
33240758
MYSM1 affects lifespan mice
| 3
MYSM1 activates lifespan mice. 3 / 3
| 3
eidos
"MYSM1 deficiency reduces the lifespan of mice ."
33240758
eidos
"Together , the findings demonstrated that Mysm1 deficiency promotes aging-related pathologies , facilitates developmental defects and early death , thereby shortening the lifespan of mice ."
33240758
eidos
"These results confirmed that Mysm1 deficiency facilitates aging , development defects , and early death , reducing the lifespan of mice ."
33240758
MYSM1 affects hemopoiesis
| 3
MYSM1 inhibits hemopoiesis. 3 / 3
| 3
reach
"Additionally, the lower representation of reverted circulating T cells might reflect a longer lifespan of the cells already present before the reversion compared with the other cell types analyzed.Our[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26220525
reach
"Mysm1 deficiency leads to abnormal hematopoiesis, hyperinflammation, enhanced viral clearance and tumor development."
36414403
reach
"In addition, disorders mainly characterized by B alymphocytosis, T lymphopenia, and hematopoiesis impairment caused by MYSM1 deficiency were found in human subjects XREF_BIBR XREF_BIBR."
26915790
MYSM1 affects TRAF3
| 1 2
MYSM1 binds TRAF3. 3 / 3
| 1 2
sparser
"Notably, TRAF2 and TRAF3—key adapters in NF-κB signaling and known to form functional complexes—were among the top hits, consistent with prior reports of MYSM1–TRAF3 or MYSM1–TRAF6 interactions xref – xref ."
41162619
sparser
"Reciprocal knockdowns demonstrated that TRAF2 depletion diminished MYSM1–TRAF3 binding, and TRAF3 depletion diminished MYSM1–TRAF2 binding (Fig. xref ), suggesting that MYSM1 engages each protein in a non–mutually exclusive manner."
41162619
reach
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
MYSM1 affects TERF1
| 3
TERF1 binds MYSM1. 3 / 3
| 3
sparser
"Gel retardation analysis with a series of mutated telomere probes revealed that the internal GGGTTT sequence in the two-telomere repeats is critical for binding of Myb-like domain of RTBP1, which is consistent with the model of the TRF1."
10811811
sparser
"TRF1 is unique in that the two Myb-like domains of the TRF1 homodimer bind to two AGGGTT sites independently and with extreme flexibility in terms of the spatial arrangement of the two target sites ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15904916
sparser
"It was suggested that in vitro the Myb-like binding domain of TRF1 binds to DNA essentially independently of the rest of the protein (König et al. xref ; Bianchi et al. xref ; Court et al. xref )."
37118559
MYSM1 affects RIPK3
| 3
MYSM1 inhibits RIPK3.
| 1
MYSM1 inhibits RIPK3. 1 / 1
| 1
reach
"Mysm1 also attenuated the I/R-mediated upregulation of Mlkl and Ripk3 mRNAs in the myocardial infarcted area (Figure S6H-I)."
39897566
MYSM1 increases the amount of RIPK3.
| 1
MYSM1 increases the amount of RIPK3. 1 / 1
| 1
reach
"MYSM1 promotes the Mlkl and Ripk3 expression by deubiquitinating and stabilizing STAT1."
39897566
MYSM1 activates RIPK3.
| 1
MYSM1 activates RIPK3. 1 / 1
| 1
reach
"Notably, STAT1 knockdown (STAT1-KD, Figure S6J) reversed MYSM1-mediated up-regulation of Mlkl and Ripk3 mRNAs (Figure 7J-K), confirming that MYSM1 transcriptionally increased the expression of necroptosis-related genes via STAT1."
39897566
MYSM1 affects RAP1
| 3
RAP1 binds MYSM1. 3 / 3
| 3
sparser
"Based on the available crystal structures, with such a short DNA duplex it is difficult to rationalize how Rap1 DBD would be able to bind with both Myb-like domains interacting with the DNA."
25637888
sparser
"The double-stranded part of the telomere is bound by Rap1 (repressor/activator-site binding protein) through its Myb-like domain, which in turn causes nucleation of a protein complex by recruiting, th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17954006
sparser
"The transient opening of the wrapping loop destabilizes this clamped structure and allows Rap1 binding to DNA through a single Myb-like domain [ xref , xref ]."
36291091
MYSM1 affects PU.1
| 3
MYSM1 binds PU.1. 3 / 3
| 3
sparser
"Through the sequential two-step ChIP assays, we found that MYSM1 was associated with PU.1 at the Pax5 locus in naïve WT B cells ( xref )."
26348977
sparser
"Mechanistically, Mysm1 interacts with Pu.1 to co-occupy the Flt3 promoter and induce Flt3 expression."
33126080
sparser
"Consistent with earlier observations ( xref ), the association of MYSM1 and PU.1 with the Pax5 locus was not detected in WT plasma cells."
26348977
MYSM1 affects PI3K
| 3
MYSM1 inhibits PI3K. 3 / 3
| 3
reach
"MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling."
34706761
reach
"Furthermore, silencing MYSM1 stimulated PI3K/AKT signaling and promoted EMT in CRC cells."
34706761
reach
"MYSM1 inhibited the CRC progression via miR-200/CDH1 and inhibition of PI3K/AKT axis [35]."
37580737
MYSM1 affects PCNA
2 | 1
MYSM1 binds PCNA. 2 / 2
2 |
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
MYSM1 affects NOD2
| 3
RIPK2 binds NOD2 and MYSM1. 2 / 2
| 2
sparser
"To explore further, we assessed by immunoprecipitation the interaction of MYSM1 with the other components in the NOD2:RIP2 complex."
30405132
sparser
"Importantly, LCL-161 did not abolish the enhanced MDP-induced NOD2:RIP2 complex assembly or phosphorylation of RIP2 and IKKα/β caused by MYSM1 deficiency (Supplementary Figure  xref )."
30405132
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 affects NFkappaB
| 2 1
MYSM1 inhibits NFkappaB.
| 2
MYSM1 inhibits NFkappaB. 2 / 2
| 2
reach
"Mechanistically, MYSM1 inhibited the NF-kappaB and MAPK signaling pathways."
39746943
reach
"MYSM1 attenuates NF-kappaB and MAPK signaling pathways in chondrocytes."
39746943
MYSM1 activates NFkappaB.
| 1
MYSM1 activates NFkappaB. 1 / 1
| 1
sparser
"Combined with the transcriptome sequencing and pathway enrichment results after knockdown of MYSM1, we further hypothesized that MYSM1 activates non-classical NF-κB in the AGE-RAGE signaling pathway and JNK and P38 signaling pathways in the MAPK signaling pathway in lung adenocarcinoma."
41162619
MYSM1 affects MPN domain
| 3
MYSM1 binds TRIM21 and MPN domain. 2 / 2
| 2
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
MYSM1 binds STING1 and MPN domain. 1 / 1
| 1
reach
"We have shown that the SWIRM domain is necessary for MYSM1 interaction with STING, and the MPN domain is necessary for STING deubiquitination.In addition to the defense against viral infections, STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects K63
| 2 1
MYSM1 binds STING1 and K63. 2 / 2
| 2
reach
"Activated MYSM1 binds to STING and removes its K63-linked polyubiquitination, repressing the synthesis of type I IFNs and pro-inflammatory cytokines, including IFN-β, IFN-stimulated gene 56 (ISG56), i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
reach
"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 binds K63. 1 / 1
| 1
sparser
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
MYSM1 affects Ig
| 3
MYSM1 inhibits Ig. 3 / 3
| 3
reach
"MYSM1 intrinsically represses Ig production by plasma cells."
26348977
reach
"Thus, these data demonstrate that MYSM1 represses plasma cell differentiation, as well as Ig production by plasma cells."
26348977
reach
"To investigate the mechanism by which MYSM1 represses plasma cell generation and Ig production, we first examined the expression of a set of transcriptional factors that are critical for plasma cell generation and Ig production XREF_BIBR XREF_BIBR XREF_BIBR by qRT-PCR assays."
26348977
MYSM1 affects IRF2
| 2 1
MYSM1 activates IRF2.
| 2
MYSM1 activates IRF2. 2 / 2
| 2
reach
"The results showed that Mysm1 significantly enhances IRF2 and IRF8 promoter activity (-600 to +50) (XREF_SUPPLEMENTARY)."
27277682
reach
"We found that IRF2 and IRF8, which are important for HSC homeostasis and commitment as transcription repressors, were expressed at lower levels in Mysm1 -/- HSCs, and Mysm1 enhanced function of the IRF2 and IRF8 promoters, suggesting that Mysm1 governs the IRFs for HSC homeostasis."
27277682
MYSM1 binds IRF2.
| 1
IRF2 binds IRF8 and MYSM1. 1 / 1
| 1
sparser
"We then tested Mysm1 binding to the promoter regions of IRF2 and IRF8 by analyzing WT (not Mysm1 −/− ) Lin − BM cells via a ChIP assay after in vitro stimulation with Poly(I:C) or IFN-γ."
27277682
MYSM1 affects His2A
| 3
MYSM1 deubiquitinates His2A.
| 2
MYSM1 deubiquitinates His2A. 2 / 2
| 2
reach
"In contrast, there was no apparent difference in ubH2A in EBF1 promoter desert region (PDR) and exon 2 regions, as well as in the osteocalcin (OC) promoter locus of WT and Mysm1 -/- Lin - progenitors, suggesting the selective deubiquitination of uH2A at the Ebf1 locus by MYSM1."
22169041
reach
"Significantly, although cells were treated with DHT as usual, the amount of pS5-Pol II on the promoter was reduced upon loss of 2A-DUB and increased levels of uH2A, which argues that 2A-DUB and result[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
MYSM1 decreases the amount of His2A.
| 1
MYSM1 decreases the amount of His2A. 1 / 1
| 1
reach
"WT 2A-DUB substantially decreased uH2A levels, whereas the D567N mutant, in which the key aspartic acid residue required for the metal-binding-dependent deubiquitinase activity was substituted to a no[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
MYSM1 affects HTH
| 3
MYSM1 binds HTH. 1 / 1
| 1
sparser
"These domains belong to the homeobox-like domain superfamily and can be categorized into three groups: the myb-like HTH domain, which binds DNA; the SANT domain, which functions as a protein–protein interaction module; and the myb-like domain, which can participate in either of these functions."
37523524
MYSM1 binds HTH and PAC. 1 / 1
| 1
sparser
"An unexpectedly potent size regulator that emerged from our screens was Dot6, a Myb-like HTH transcription factor that binds to the PAC (Polymerase A and C) motif [ xref ]."
30921326
MYSM1 binds MYB and HTH. 1 / 1
| 1
sparser
"Both genes contained two introns, encoded 257 amino acids with two-Aa difference, and carried the unabridged HTH-MYB, MYB-like DNA-binding, and SANT domains."
31035916
MYSM1 affects FLT3
| 2 1
MYSM1 increases the amount of FLT3.
| 2
MYSM1 increases the amount of FLT3. 2 / 2
| 2
reach
"Mechanistically, Mysm1 interacts with Pu.1 to co-occupy the Flt3 promoter and induce Flt3 expression."
33126080
reach
"Similarly, in mouse hematopoietic stem or progenitor cells (HSPCs) MYSM1 was shown to promote the expression of Gfi1, Flt3, Id2, Ebf1 and Pax5 genes, important for the normal progression of hematopoiesis, also via H2A-K119ub deubiquitination of their promoters and other regulatory elements ."
36611064
MYSM1 binds FLT3.
| 1
MYSM1 binds FLT3. 1 / 1
| 1
sparser
"MYSM1 bound to the Flt3 gene promoter, and loss of MYSM1 resulted in reduced recruitment of the major hematopoietic transcription factor PU.1 and an increase in repressive histone marks H2AK119ub and H3K27me3 at the Flt3 locus [ xref ] ( xref )."
32344625
MYSM1 affects Cell Hypoxia
| 3
MYSM1 activates Cell Hypoxia. 3 / 3
| 3
reach
"These results validated that MYSM1 contributes to H/R-induced cardiomyocyte injury in vitro."
39897566
reach
"Loss- and gain-of-function analysis further confirmed that MYSM1 promotes H/R-induced cardiomyocyte injury in vitro."
39897566
reach
"MYSM1 promotes H/R-induced cardiomyocyte injury in vitro."
39897566
MYSM1 affects CDH1
| 3
MYSM1 inhibits CDH1.
| 2
MYSM1 inhibits CDH1. 2 / 2
| 2
reach
"MYSM1 inhibited the CRC progression via miR-200/CDH1 and inhibition of PI3K/AKT axis [35]."
37580737
reach
"MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling."
34706761
MYSM1 decreases the amount of CDH1.
| 1
MYSM1 decreases the amount of CDH1. 1 / 1
| 1
reach
"Mechanistically, MYSM1 directly bound to the promoter region of miR-200/CDH1, impaired the enrichment of repressive H2AK119ub1 modification and epigenetically enhanced miR-200/CDH1 expression."
34706761
MYSM1 affects BLNK
| 3
MYSM1 activates BLNK. 3 / 3
| 3
reach
"MYSM1 depletion suppresses cell proliferation and increases antiestrogen sensitivity in BCa-derived cell lines."
38177530
reach
"MYSM1 depletion attenuates BCa-derived cell growth in xenograft models and increases the sensitivity of antiestrogen agents in BCa cells."
38177530
reach
"Above all, the results indicate that MYSM1 promotes cell proliferation and G1-S phase transition in BCa, and the effect of MYSM1 on cell growth is partially related to ERα pathway.To test the functional of MYSM1 on BCa cell growth in vivo, MCF-7 cells infected with shMYSM1 or negative control lentivirus (shCtrl) were individually implanted subcutaneously into flank sides of 4-week-old female BALB/c nude mice (n = 7) armpit."
38177530
MPN domain affects MYSM1
| 3
MYSM1 binds TRIM21 and MPN domain. 2 / 2
| 2
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
MYSM1 binds STING1 and MPN domain. 1 / 1
| 1
reach
"We have shown that the SWIRM domain is necessary for MYSM1 interaction with STING, and the MPN domain is necessary for STING deubiquitination.In addition to the defense against viral infections, STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
K63 affects MYSM1
| 2 1
MYSM1 binds STING1 and K63. 2 / 2
| 2
reach
"Activated MYSM1 binds to STING and removes its K63-linked polyubiquitination, repressing the synthesis of type I IFNs and pro-inflammatory cytokines, including IFN-β, IFN-stimulated gene 56 (ISG56), i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
reach
"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 binds K63. 1 / 1
| 1
sparser
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
Infections affects MYSM1
| 3
Infections activates MYSM1. 3 / 3
| 3
reach
"It has been recently reported that HSV-1 infection upregulates metalloprotease, myb-like, SWIRM, and MPN domains 1 protein (MYSM1) to inhibit STING activation via de-ubiquitinating STING (39)."
38639486
reach
"Moreover, HSV-1 infection upregulated Mysm1 mRNA in the blood of C56BL/6 mice ( Figures 1 H and S1 A)."
33086059
reach
"Considering the double-stranded DNA nature of the Ad genome, infection by DNA virus can induce the upregulation of deubiquitinase MYSM1."
37766208
HTH affects MYSM1
| 3
MYSM1 binds HTH. 1 / 1
| 1
sparser
"These domains belong to the homeobox-like domain superfamily and can be categorized into three groups: the myb-like HTH domain, which binds DNA; the SANT domain, which functions as a protein–protein interaction module; and the myb-like domain, which can participate in either of these functions."
37523524
MYSM1 binds HTH and PAC. 1 / 1
| 1
sparser
"An unexpectedly potent size regulator that emerged from our screens was Dot6, a Myb-like HTH transcription factor that binds to the PAC (Polymerase A and C) motif [ xref ]."
30921326
MYSM1 binds MYB and HTH. 1 / 1
| 1
sparser
"Both genes contained two introns, encoded 257 amino acids with two-Aa difference, and carried the unabridged HTH-MYB, MYB-like DNA-binding, and SANT domains."
31035916
EP300 affects MYSM1
1 | 2
MYSM1 binds EP300 and CREBBP. 2 / 2
| 2
sparser
"2A-DUB associates with histone acetyltransferase p300/CBP-associated factor (PCAF/KAT2B) in a coregulatory protein complex and regulates transcription by coordinating histone acetylating, deubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18929679
sparser
"2A-DUB associates with the histone acetyltransferase p300/CBP-associated factor (p/CAF), a complex that mediates acetylation of histones H3 and H4 in transcriptionally active chromatin ( xref )."
19489724
EP300 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
38177530
CREBBP affects MYSM1
1 | 2
MYSM1 binds EP300 and CREBBP. 2 / 2
| 2
sparser
"2A-DUB associates with histone acetyltransferase p300/CBP-associated factor (PCAF/KAT2B) in a coregulatory protein complex and regulates transcription by coordinating histone acetylating, deubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18929679
sparser
"2A-DUB associates with the histone acetyltransferase p300/CBP-associated factor (p/CAF), a complex that mediates acetylation of histones H3 and H4 in transcriptionally active chromatin ( xref )."
19489724
CREBBP binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
38177530
ASGR1 affects MYSM1
| 3
ASGR1 activates MYSM1. 3 / 3
| 3
reach
"We constructed MYSM1-KD HL-1 cells and overexpressed MYSM1 by transfecting Flag-MYSM1 (oeMYSM1) in HL-1 cells, followed by treatment with DOX (1 µM)."
39695708
reach
"We constructed MYSM1-knockdown (KD) HL-1 cells and overexpressed MYSM1 by transfecting Flag-MYSM1 (oeMYSM1) in HL-1 cells, followed by treatment with DOX (1 µM)."
39695708
reach
"Firstly, we analyzed the binding proteins of MYSM1 by Co-IP with LC-MS/MS (interactomics analysis) in HL-1 cells overexpressing MYSM1 (Figure 5A upper and Figure 5B)."
39897566
2'-deoxyadenosine affects MYSM1
| 3
reach
"Similarly, in mouse bone marrow-derived macrophages (BMDMs), Mysm1 mRNA was activated by HSV-1 ( Figure 1 E), poly(dA:dT), ISD 45, DNA90, and HSV120 ( Figure 1 F), as well as by cGAMP ( Figure 1 G)."
33086059
reach
"In addition, in C57BL/6 wild-type (WT) mouse embryonic fibroblasts (MEFs), Mysm1 mRNA was induced by HSV-1 ( Figure 1 C), poly(dA:dT), ISD 45, DNA 90, and HSV120 ( Figure 1 D)."
33086059
reach
"The results indicated that Mysm1 mRNA was induced by HSV-1 ( Figure 1 A) and poly(dA:dT) ( Figure 1 B) in human acute monocytic leukemia (THP-1) cell-derived macrophages."
33086059
Treatments DOX affects MYSM1
| 2
Treatments DOX inhibits MYSM1.
| 1
Treatments DOX inhibits MYSM1. 1 / 1
| 1
eidos
"In human primary diploid fibroblasts ( WI-38 ) , treatments with ETO and DOX increased Mysm1 expression after 2 and 5 days , but decreased Mysm1 expression at 8 days ( Figure S2A , B , Supporting Information ) ."
33240758
Treatments DOX activates MYSM1.
| 1
Treatments DOX activates MYSM1. 1 / 1
| 1
eidos
"In human primary diploid fibroblasts ( WI-38 ) , treatments with ETO and DOX increased Mysm1 expression after 2 and 5 days , but decreased Mysm1 expression at 8 days ( Figure S2A , B , Supporting Information ) ."
33240758
OeMYSM1 affects MYSM1
| 2
OeMYSM1 activates MYSM1. 2 / 2
| 2
reach
"We constructed MYSM1-knockdown (KD) HL-1 cells and overexpressed MYSM1 by transfecting Flag-MYSM1 (oeMYSM1) in HL-1 cells, followed by treatment with DOX (1 µM)."
39695708
reach
"We constructed MYSM1-KD HL-1 cells and overexpressed MYSM1 by transfecting Flag-MYSM1 (oeMYSM1) in HL-1 cells, followed by treatment with DOX (1 µM)."
39695708
NmoT affects MYSM1
| 2
MYSM1 binds nmoT. 2 / 2
| 2
sparser
"For zebrafish Harbinger3_DR , it is also known that the Myb-like protein interacts physically with the transposase and this interaction is necessary for the colocalization of both proteins to the nucleus [ xref ]."
36451208
sparser
"The Myb-like protein is required for transposition and physically interacts with the N-terminal region of the transposase via its C-terminal domain."
18339812
Ferrostatin-1 affects MYSM1
| 2
Ferrostatin-1 activates MYSM1. 1 / 1
| 1
reach
"Recently, Zhao et al. demonstrated that histone deubiquitinase MYSM1 (Myb-like, SWIRM and MPN domains 1) deficiency reduced the translation of ferroptosis-protective mRNAs, resulting in increased ferroptosis of human hematopoietic stem cells (HSCs), and HSC population maintenance was fully restored by the ferroptosis inhibitors (e.g., deferoxamine (DFO), ferrostatin-1 (Fer-1), and vitamin E)16."
37497000
Ferrostatin-1 activates MYSM1. 1 / 1
| 1
reach
"Recently, Zhao et al. demonstrated that histone deubiquitinase MYSM1 (Myb-like, SWIRM and MPN domains 1) deficiency reduced the translation of ferroptosis-protective mRNAs, resulting in increased ferroptosis of human hematopoietic stem cells (HSCs), and HSC population maintenance was fully restored by the ferroptosis inhibitors (e.g., deferoxamine (DFO), ferrostatin-1 (Fer-1), and vitamin E)16."
37497000
End3 affects MYSM1
| 2
End3 binds MYSM1. 2 / 2
| 2
reach
"USP22 and 2A-DUB are enzymatically active as part of the SAGA and 2A-DUB complexes respectively [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
20974139
reach
"Both the 2A-DUB and SAGA complexes also harbor histone acetyltransferase (HAT) subunits, PCAF and its homologue GCN5, respectively."
20974139
Bentiromide affects MYSM1
| 2
Bentiromide inhibits MYSM1.
| 1
Bentiromide inhibits MYSM1. 1 / 1
| 1
reach
"Consistently, microinjection of PFTα in the MHb attenuated the antidepressant effect of Mysm1 downregulation."
36414403
Bentiromide activates MYSM1.
| 1
Bentiromide activates MYSM1. 1 / 1
| 1
reach
"Pifithrin alpha (PFT alpha) and Compound C, antagonists of p53 and AMPK, respectively, repressed ATP production and reversed the antidepressant effect of Mysm1 knockdown."
36414403
TRIM21 affects SWIRM domain
| 2
MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
| 2
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
TRIM21 affects MPN domain
| 2
MYSM1 binds TRIM21 and MPN domain. 2 / 2
| 2
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
TRAF6 affects MYSM1
| 1 1
MYSM1 binds TRAF6. 2 / 2
| 1 1
reach
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
sparser
"Notably, TRAF2 and TRAF3—key adapters in NF-κB signaling and known to form functional complexes—were among the top hits, consistent with prior reports of MYSM1–TRAF3 or MYSM1–TRAF6 interactions xref – xref ."
41162619
TMPRSS11D affects MYSM1
| 2
MYSM1 binds USP22 and TMPRSS11D. 1 / 1
| 1
sparser
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
TMPRSS11D binds MYSM1. 1 / 1
| 1
sparser
"To test whether p/CAF, a HAT that interacts with 2A-DUB and augments the H2A deubiquitinase activity of 2A-DUB ( Figures 2 and 3 ), plays a role in 2A-DUB-dependent activation, we compared 2A-DUB-indu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
SWIRM domain affects TRIM21
| 2
MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
| 2
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
SWIRM domain affects STAT1
| 2
MYSM1 binds STAT1 and SWIRM domain. 2 / 2
| 2
reach
"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
39897566
reach
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
STING1 affects K63
| 2
MYSM1 binds STING1 and K63. 2 / 2
| 2
reach
"Activated MYSM1 binds to STING and removes its K63-linked polyubiquitination, repressing the synthesis of type I IFNs and pro-inflammatory cytokines, including IFN-β, IFN-stimulated gene 56 (ISG56), i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
reach
"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
STAT1 affects SWIRM domain
| 2
MYSM1 binds STAT1 and SWIRM domain. 2 / 2
| 2
reach
"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
39897566
reach
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
SPI1 affects MYSM1
| 1 1
MYSM1 binds SPI1. 2 / 2
| 1 1
reach
"MYSM1 interacts with the transcription factor PU.1 for its recruitment to the Pax5 locus."
26348977
sparser
"MYSM1 interacts with the transcription factor PU.1 for its recruitment to the Pax5 locus."
26348977
RNF31 affects MYSM1
| 2
TAB3 binds TAB2, MYSM1, and RNF31. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
RBM10 affects MYSM1
1 | 1
MYSM1 binds RBM10. 2 / 2
1 | 1
sparser
"Our transcriptomic analyses, which show no effect of RBM10 downregulation on alternative splicing in GLC20 cells, are supported by (1) our RBM10-only targets ( xref ), which are involved in various aspects of gene expression regulation, excluding alternative splicing, (2) RBM10’s mRNA stabilization effect on its only identified direct RNA target, the AT1 receptor [ xref ], and (3) RBM10’s interaction with the 2A-DUB deubiquitinase protein complex [ xref ] and the Rac-specific GTPase-activitating protein FilGAP [ xref ]."
28662214
biogrid
No evidence text available
17707232
PAX3 affects MYSM1
| 1 1
MYSM1 binds PAX3. 2 / 2
| 1 1
sparser
"No significant binding of MYSM1 to either the PAX3 or the ETS1 promoter sequence around the transcriptional start site (TSS) could be detected in comparison to IgG controls."
28978033
reach
"In context with our finding that MYSM1 bound to the c-MET promoter region in close vicinity to PAX3 in melanoma cells, our data indicate that MYSM1 is an epigenetic regulator of melanoma growth and potentially promising new target for tumor therapy."
28978033
PARP1 affects MYSM1
1 | 1
PARP1 binds MYSM1. 2 / 2
1 | 1
sparser
"This study identified the role of the MYSM1-PARP1 axis in mediating cardiac hypertrophy and suggested that MYSM1 is a promising pharmacological target for the treatment of cardiac hypertrophy."
39907013
biogrid
No evidence text available
34079125
MYSM1 affects 𝛾 -H2AX
| 2
MYSM1 decreases the amount of 𝛾 -H2AX.
| 1
MYSM1 decreases the amount of 𝛾 -H2AX. 1 / 1
| 1
reach
"However, MYSM1 knockdown did not influence DOX-induced 𝛾 -H2AX expression (Fig. S4C), suggesting that DNA damage might not be one of the pathological conditions in MYSM1-mediated cardiotoxicity.Analogous to the loss-of-function assessment of MYSM1, the gain-of-function of MYSM1 was evaluated by transfecting HL-1 cells with Flag-MYSM1 plasmid (oeMYSM1, Fig. S4D)."
39695708
MYSM1 activates 𝛾 -H2AX.
| 1
MYSM1 activates 𝛾 -H2AX. 1 / 1
| 1
reach
"Therefore, we investigated whether MYSM1 mediates DOX-induced DNA damage in cardiomyocytes by detecting 𝛾 -H2AX."
39695708
MYSM1 affects ubH2A
| 2
MYSM1 deubiquitinates ubH2A.
| 1
MYSM1 deubiquitinates ubH2A. 1 / 1
| 1
reach
"Collectively, these data indicate that MYSM1 not only deubiquitinates ubH2A, but also orchestrates histone modifications at the Ebf1 locus and that its deficiency results in the repressive histone modifications and impaired recruitment of the key transcription factors to the Ebf1 locus."
22169041
MYSM1 decreases the amount of ubH2A.
| 1
MYSM1 decreases the amount of ubH2A. 1 / 1
| 1
reach
"Knockdown of 2A-DUB by siRNA in cell culture increases the global levels of ubH2A."
18374642
reach
"Furthermore, silencing MYSM1 stimulated PI3K/AKT signaling and promoted EMT in CRC cells."
34706761
reach
"MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling."
34706761
MYSM1 affects pathway
| 2
MYSM1 inhibits pathway.
| 1
MYSM1 inhibits pathway. 1 / 1
| 1
sparser
"These results indicate that MYSM1 inhibits the cGAS-STING pathway by interacting with the STING N terminus via the SWIRM domain."
33086059
MYSM1 activates pathway.
| 1
MYSM1 activates pathway. 1 / 1
| 1
sparser
"Rescue experiments indicate that inhibiting TRAF2 or TRAF3 can attenuate MYSM1-induced activation of the MAPK pathway."
41162619
MYSM1 affects p38
| 2
MYSM1 inhibits p38.
| 1
MYSM1 inhibits p38. 1 / 1
| 1
reach
"MYSM1-deficient cells have increased p38 activation, cell cycle arrest, and increased apoptosis, presumably due to altered transcriptional activity."
29731008
MYSM1 increases the amount of p38.
| 1
MYSM1 increases the amount of p38. 1 / 1
| 1
reach
"To confirm MYSM1’s specific role in these signaling pathways, Mysm1 plasmids were reintroduced into Mysm1-KO chondrocytes, significantly restoring the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 (Fig. S4D-E)."
39746943
MYSM1 affects nmoT
| 2
MYSM1 binds nmoT. 2 / 2
| 2
sparser
"For zebrafish Harbinger3_DR , it is also known that the Myb-like protein interacts physically with the transposase and this interaction is necessary for the colocalization of both proteins to the nucleus [ xref ]."
36451208
sparser
"The Myb-like protein is required for transposition and physically interacts with the N-terminal region of the transposase via its C-terminal domain."
18339812
MYSM1 affects malonaldehyde
| 2
MYSM1 increases the amount of malonaldehyde. 2 / 2
| 2
reach
"Additionally, MYSM1 knockdown diminished the elevated MDA levels induced by DOX (Fig. 7B) and augmented both GSH levels and SOD activity (Fig. 7C-D)."
39695708
reach
"Conversely, the upregulation of MYSM1 further augmented DOX- induced the increase in Fe content (Fig. 7E), elevated the MDA levels (Fig. 7F), and diminished the GSH levels and SOD activity (Fig. 7G-H)."
39695708
MYSM1 affects histone H2AK119ub
| 2
MYSM1 inhibits histone H2AK119ub. 2 / 2
| 2
eidos
"At many of these loci , loss of MYSM1 resulted in increased binding of PRC1 complex proteins and elevated levels of histone H2AK119ub [ 20,30,32,33,34 ] , suggesting that MYSM1 antagonizes PRC1-mediated histone ubiquitination and transcriptional repression ."
32344625
eidos
"MYSM1 was required for normal recruitment of transcription factors GATA2 and RUNX1 to the Gfi1 locus , while loss of MYSM1 resulted in increased recruitment of the PRC1 complex proteins RING1B and BMI1 and increased levels of histone H2AK119ub [ 34 ] ( Figure 2 ) ."
32344625
MYSM1 affects glutathione
| 2
MYSM1 increases the amount of glutathione.
| 1
MYSM1 increases the amount of glutathione. 1 / 1
| 1
reach
"Additionally, MYSM1 knockdown diminished the elevated MDA levels induced by DOX (Fig. 7B) and augmented both GSH levels and SOD activity (Fig. 7C-D)."
39695708
MYSM1 decreases the amount of glutathione.
| 1
MYSM1 decreases the amount of glutathione. 1 / 1
| 1
reach
"Conversely, the upregulation of MYSM1 further augmented DOX- induced the increase in Fe content (Fig. 7E), elevated the MDA levels (Fig. 7F), and diminished the GSH levels and SOD activity (Fig. 7G-H)."
39695708
MYSM1 affects gene expression
| 2
reach
"Monoubiquitination of H2A on lysine 119 (H2AK119Ub) represses transcription, and MYSM1 catalyzes removal of this ubiquitin moiety to initiate gene expression."
38065233
reach
"9 One of the main known MYSM1 substrates is histone H2A, monoubiquitinated at K119, and MYSM1 catalytic activity on this epigenetic mark promotes the activation of gene expression."
34114734
MYSM1 affects end3
| 2
End3 binds MYSM1. 2 / 2
| 2
reach
"USP22 and 2A-DUB are enzymatically active as part of the SAGA and 2A-DUB complexes respectively [XREF_BIBR, XREF_BIBR, XREF_BIBR]."
20974139
reach
"Both the 2A-DUB and SAGA complexes also harbor histone acetyltransferase (HAT) subunits, PCAF and its homologue GCN5, respectively."
20974139
MYSM1 inhibits deoxyribonucleic acid. 2 / 2
| 2
eidos
"C-E ) WT MEFs and Mysm1 - / - MEFs ( C , D ) or WT MEFs , Mysm1 - / - MEFs , and Mysm1 - / - MEFs transfected with Flag-Mysm1 ( E ) were treated with DOX to induce DNA damage ."
33240758
eidos
"Our data revealed a distinct mechanism through which MYSM1 eliminates DNA damage by promoting DNA repair to suppress senescence and the aging processes ."
33240758
MYSM1 affects cellular senescence aging process human mice primary cells mice organs
| 2
MYSM1 inhibits cellular senescence aging process human mice primary cells mice organs. 2 / 2
| 2
eidos
"Detailed functional analyses confirmed that MYSM1 represses cellular senescence and the aging process in human and mice primary cells and in mice organs ."
33240758
eidos
"MYSM1 functionally represses cellular senescence and the aging process in human and mice primary cells and in mice organs ."
33240758
MYSM1 affects cell quiescence
| 2
MYSM1 inhibits cell quiescence. 2 / 2
| 2
reach
"33 Using Ki67 as a marker of non quiescent cells, we show that Mysm1 -/- HSC loss of quiescence is rescued in Mysm1 -/- p53 -/- HSCs (XREF_FIG, right)."
26768662
reach
"Overall, this suggests that continued dysregulation of p53 effector functions beyond the upregulation of Bbc3 and PUMA contributes to persistent Mysm1 -/- HSC loss of quiescence, and also to the lymphoid differentiation arrest of Mysm1 -/- Puma -/- MPP4s."
26768662
MYSM1 affects TRAF6
| 1 1
MYSM1 binds TRAF6. 2 / 2
| 1 1
reach
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
sparser
"Notably, TRAF2 and TRAF3—key adapters in NF-κB signaling and known to form functional complexes—were among the top hits, consistent with prior reports of MYSM1–TRAF3 or MYSM1–TRAF6 interactions xref – xref ."
41162619
MYSM1 affects TMPRSS11D
| 2
MYSM1 binds USP22 and TMPRSS11D. 1 / 1
| 1
sparser
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
TMPRSS11D binds MYSM1. 1 / 1
| 1
sparser
"To test whether p/CAF, a HAT that interacts with 2A-DUB and augments the H2A deubiquitinase activity of 2A-DUB ( Figures 2 and 3 ), plays a role in 2A-DUB-dependent activation, we compared 2A-DUB-indu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
MYSM1 affects THR
| 2
MYSM1 increases the amount of THR.
| 1
MYSM1 increases the amount of THR. 1 / 1
| 1
reach
"In contrast, RARbeta2, a known target gene of retinoic acid receptor alpha (RARalpha), was not detectably regulated by 2A-DUB, and thyroid hormone receptor (T 3 R)-mediated transcription was repressed[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
MYSM1 binds THR.
| 1
MYSM1 binds THR. 1 / 1
| 1
reach
"Histone H2A deubiquitinase (2A-DUB) forms a complex with approximately 10 proteins such as histone acetyltransferase p300/CBP-associated factor (p/CAF; presently, PCAF), thyroid hormone receptor-interacting protein 5 (Trip5) and RBM10 (Zhu et al., 2007)."
33515724
MYSM1 affects SYT1
| 2
MYSM1 increases the amount of SYT1.
| 1
MYSM1 increases the amount of SYT1. 1 / 1
| 1
reach
"To confirm MYSM1’s specific role in these signaling pathways, Mysm1 plasmids were reintroduced into Mysm1-KO chondrocytes, significantly restoring the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 (Fig. S4D-E)."
39746943
MYSM1 decreases the amount of SYT1.
| 1
MYSM1 decreases the amount of SYT1. 1 / 1
| 1
reach
"MYSM1 overexpression significantly decreased the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 under IL-1β treatment at various time points (Fig. 3a, b, d, e)."
39746943
MYSM1 affects SWIRM domain, and TRIM21
| 2
MYSM1 binds TRIM21 and SWIRM domain. 2 / 2
| 2
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
MYSM1 affects STAT1, and SWIRM domain
| 2
MYSM1 binds STAT1 and SWIRM domain. 2 / 2
| 2
reach
"As depicted in Figure 6G, STAT1 interacted with MYSM1-WT, MYSM1-△SANT, and MYSM1-△MPN, but failed to bind to MYSM1-△SWIRM, indicating that MYSM1 binds to STAT1 through the SWIRM domain."
39897566
reach
"Consistently, our data showed that MYSM1 binds to STAT1 mainly through the SWIRM domain."
39897566
MYSM1 affects SPI1
| 1 1
MYSM1 binds SPI1. 2 / 2
| 1 1
reach
"MYSM1 interacts with the transcription factor PU.1 for its recruitment to the Pax5 locus."
26348977
sparser
"MYSM1 interacts with the transcription factor PU.1 for its recruitment to the Pax5 locus."
26348977
MYSM1 inhibits Receptors, Pattern Recognition. 2 / 2
| 2
reach
"Thus, the ability of MYSM1 to promptly amass in the cytoplasm, inhibit proximal PRR signaling events, and then undergo proteasomal degradation highlights the role of MYSM1 as part of highly versatile [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26474655
reach
"Together these results indicate that MYSM1 negatively regulates PRR mediated innate immune responses via the cooperative activity of two domains: the SWIRM domain, which mediates interaction with TRAF[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26474655
MYSM1 affects RNF31
| 2
TAB3 binds TAB2, MYSM1, and RNF31. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
MYSM1 affects RBM10
1 | 1
MYSM1 binds RBM10. 2 / 2
1 | 1
sparser
"Our transcriptomic analyses, which show no effect of RBM10 downregulation on alternative splicing in GLC20 cells, are supported by (1) our RBM10-only targets ( xref ), which are involved in various aspects of gene expression regulation, excluding alternative splicing, (2) RBM10’s mRNA stabilization effect on its only identified direct RNA target, the AT1 receptor [ xref ], and (3) RBM10’s interaction with the 2A-DUB deubiquitinase protein complex [ xref ] and the Rac-specific GTPase-activitating protein FilGAP [ xref ]."
28662214
biogrid
No evidence text available
17707232
MYSM1 affects PRSS27
| 2
MYSM1 binds PRSS27. 2 / 2
| 2
sparser
"Virtual screening identified Imatinib as a small molecule capable of interacting with the catalytic MPN domain of MYSM1, and this effectively inhibited breast cancer cell growth, highlighting the MYSM1-ERα axis as a promising target to overcome endocrine independence in breast cancer [ xref ]."
| PMC
sparser
"A virtual screen shows that the small molecule Imatinib could potentially interact with catalytic MPN domain of MYSM1 to inhibit BCa cell growth via MYSM1-ERα axis."
38177530
MYSM1 affects PAX3
| 1 1
MYSM1 binds PAX3. 2 / 2
| 1 1
sparser
"No significant binding of MYSM1 to either the PAX3 or the ETS1 promoter sequence around the transcriptional start site (TSS) could be detected in comparison to IgG controls."
28978033
reach
"In context with our finding that MYSM1 bound to the c-MET promoter region in close vicinity to PAX3 in melanoma cells, our data indicate that MYSM1 is an epigenetic regulator of melanoma growth and potentially promising new target for tumor therapy."
28978033
MYSM1 affects Osteoarthritis
| 2
MYSM1 inhibits Osteoarthritis.
| 1
MYSM1 inhibits Osteoarthritis. 1 / 1
| 1
reach
"MYSM1 attenuates osteoarthritis by recruiting PP2A to deubiquitinate and dephosphorylate RIPK2."
39746943
MYSM1 binds Osteoarthritis.
| 1
reach
"The possible association between MYSM1 and OA was further examined in vivo."
39746943
MYSM1 affects NOD2, and RIPK2
| 2
RIPK2 binds NOD2 and MYSM1. 2 / 2
| 2
sparser
"To explore further, we assessed by immunoprecipitation the interaction of MYSM1 with the other components in the NOD2:RIP2 complex."
30405132
sparser
"Importantly, LCL-161 did not abolish the enhanced MDP-induced NOD2:RIP2 complex assembly or phosphorylation of RIP2 and IKKα/β caused by MYSM1 deficiency (Supplementary Figure  xref )."
30405132
MYSM1 affects MMP3
| 2
MYSM1 inhibits MMP3.
| 1
MYSM1 inhibits MMP3. 1 / 1
| 1
reach
"Histomorphometry results indicated that Mysm1 deletion enhanced MMP13 and MMP3 positive chondrocytes in both sham and DMM groups (Fig. 2g–i)."
39746943
MYSM1 decreases the amount of MMP3.
| 1
MYSM1 decreases the amount of MMP3. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects MMP13
| 2
MYSM1 inhibits MMP13.
| 1
MYSM1 inhibits MMP13. 1 / 1
| 1
reach
"Histomorphometry results indicated that Mysm1 deletion enhanced MMP13 and MMP3 positive chondrocytes in both sham and DMM groups (Fig. 2g–i)."
39746943
MYSM1 decreases the amount of MMP13.
| 1
MYSM1 decreases the amount of MMP13. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects MLKL
| 2
MYSM1 increases the amount of MLKL.
| 1
MYSM1 increases the amount of MLKL. 1 / 1
| 1
reach
"MYSM1 promotes the Mlkl and Ripk3 expression by deubiquitinating and stabilizing STAT1."
39897566
MYSM1 activates MLKL.
| 1
MYSM1 activates MLKL. 1 / 1
| 1
reach
"Notably, STAT1 knockdown (STAT1-KD, Figure S6J) reversed MYSM1-mediated up-regulation of Mlkl and Ripk3 mRNAs (Figure 7J-K), confirming that MYSM1 transcriptionally increased the expression of necroptosis-related genes via STAT1."
39897566
MYSM1 affects MET
| 2
MYSM1 binds MET. 2 / 2
| 2
sparser
"In support of a collaboration of MYSM1 with PAX3 in transcriptional regulation, MYSM1 binding to the c-MET promoter in melanoma cells occurred in vicinity to the PAX3 consensus sequence previously described [ xref ]."
28978033
sparser
"In context with our finding that MYSM1 bound to the c-MET promoter region in close vicinity to PAX3 in melanoma cells, our data indicate that MYSM1 is an epigenetic regulator of melanoma growth and potentially promising new target for tumor therapy."
28978033
MYSM1 affects JNK
| 2
MYSM1 increases the amount of JNK.
| 1
MYSM1 increases the amount of JNK. 1 / 1
| 1
reach
"To confirm MYSM1’s specific role in these signaling pathways, Mysm1 plasmids were reintroduced into Mysm1-KO chondrocytes, significantly restoring the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 (Fig. S4D-E)."
39746943
MYSM1 decreases the amount of JNK.
| 1
MYSM1 decreases the amount of JNK. 1 / 1
| 1
reach
"MYSM1 overexpression significantly decreased the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 under IL-1β treatment at various time points (Fig. 3a, b, d, e)."
39746943
MYSM1 affects ISRE-Luc
| 2
MYSM1 inhibits ISRE-Luc. 2 / 2
| 2
reach
"Our results also showed that the WT MYSM1 and MYSM1 lack of SANT domain strongly suppressed cGAS-STING-induced ISRE-Luc activities, whereas the MYSM1 lack of SWIRM domain failed to suppress cGAS-STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
reach
"The cGAS-STING-induced ISRE-Luc and NF-κB-Luc activities ( Figure 3 A), as well as ISRE activity induced by cGAS-STING or STING ( Figure 3 B), were suppressed by MYSM1."
33086059
MYSM1 affects IL6
| 2
MYSM1 inhibits IL6. 2 / 2
| 2
reach
"Increased MYSM1 can decrease type I IFN, IL-6, and other inflammatory cytokines in SLE mice (86)."
35603197
reach
"Interestingly, a recovery experiment demonstrated that IFN-β , IL-6, and Cxcl10 mRNA induced by HSV-1 in MEFs derived from Mysm1 −/− mice were significantly suppressed by the overexpression of MYSM1 ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects IL3
| 1 1
MYSM1 binds IL3. 2 / 2
| 1 1
sparser
"MYSM1 interacts with nuclear factor IL-3 (NFIL3, also known as E4BP4), a critical factor for mouse NK cell development, and the recruitment of nuclear factor Il-3 to the ID2 locus is dependent on MYSM1."
24062447
reach
"MYSM1 interacts with nuclear factor IL-3 (NFIL3, also known as E4BP4), a critical factor for mouse NK cell development, and the recruitment of nuclear factor Il-3 to the ID2 locus is dependent on MYSM1."
24062447
MYSM1 affects ID2
| 2
MYSM1 increases the amount of ID2. 2 / 2
| 2
reach
"Similarly, in mouse hematopoietic stem or progenitor cells (HSPCs) MYSM1 was shown to promote the expression of Gfi1, Flt3, Id2, Ebf1 and Pax5 genes, important for the normal progression of hematopoiesis, also via H2A-K119ub deubiquitination of their promoters and other regulatory elements ."
36611064
reach
"MYSM1 was shown to promote expression of Ebf1, Pax5, and Id2, key transcriptional regulators of B-cell commitment and differentiation."
38065233
MYSM1 affects Histone_H2A
| 2
MYSM1 deubiquitinates Histone_H2A. 2 / 2
| 2
reach
"1 , 2 MYSM1 functions in deubiquitinating the histone 2A ubiquitinated at position K119, an epigenetic marker for gene transcription silencing."
39233474
reach
"MYSM1, a member of the JAMM family, is a histone DUB which specifically deubiquitinates histone 2A [129]."
30123285
MYSM1 affects Heart Failure
| 2
MYSM1 activates Heart Failure. 2 / 2
| 2
reach
"Within this dataset, the mRNA expression of MYSM1 was elevated in heart failure (HF) group compared to the control normal heart (NHF) group."
39897566
reach
"Similarly, we observed that the mRNA expression of MYSM1, EIF3F, STAMBP, and BRCC3 were elevated in heart failure (HF) group compared to the control normal heart (NHF) group (GSE133054, Fig. 1B)."
39695708
MYSM1 affects H3C12
2 |
H3C12 binds MYSM1. 2 / 2
2 |
biogrid
No evidence text available
29568061
biogrid
No evidence text available
36089195
MYSM1 affects H2AX
2 |
MYSM1 binds H2AX. 2 / 2
2 |
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
MYSM1 affects GFI1
| 1 1
MYSM1 increases the amount of GFI1.
| 1
MYSM1 increases the amount of GFI1. 1 / 1
| 1
reach
"Similarly, in mouse hematopoietic stem or progenitor cells (HSPCs) MYSM1 was shown to promote the expression of Gfi1, Flt3, Id2, Ebf1 and Pax5 genes, important for the normal progression of hematopoiesis, also via H2A-K119ub deubiquitination of their promoters and other regulatory elements ."
36611064
MYSM1 binds GFI1.
| 1
MYSM1 binds GFI1. 1 / 1
| 1
sparser
"We found that Mysm1 directly associates with the Gfi1 enhancer element and promotes its transcription through Gata2 and Runx1 transactivation."
24014243
MYSM1 affects DREB2A
| 2
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
DREB2A binds MYSM1. 1 / 1
| 1
sparser
"We recently identified mediator in Arabidopsis thaliana and we have also reported that the Med25 subunit in Arabidopsis interacts with three different transcription factors, Dreb2a, Zfhd1 and myb-like which all are involved in different stress response pathways ( xref , xref ) In this article, we use a set of biochemical and biophysical methods to study interaction between the Med25 mediator subunit and the transcription factor Dreb2a from A. thaliana ."
22447446
MYSM1 affects DNA accumulation
| 2
MYSM1 inhibits DNA accumulation. 2 / 2
| 2
eidos
"Taken together , the results demonstrate that MYSM1 prevents DNA damage accumulation by facilitating HR mediated DNA repair ."
33240758
eidos
"Together , the results suggested that Mysm1 expression is associated with senescence , and that MYSM1 prevents DNA damage accumulation to suppress senescence and aging by facilitating the HR mediated DNA repair ."
33240758
MYSM1 affects DDR-associated SASP
| 2
MYSM1 inhibits DDR-associated SASP. 2 / 2
| 2
eidos
"MYSM1 suppresses DDR-associated SASP ."
33240758
eidos
"Taken together , these results showed that MYSM1 represses DDR-associated SASP ."
33240758
MYSM1 affects Cell Survival
| 2
MYSM1 inhibits Cell Survival. 2 / 2
| 2
reach
"Notably, MYSM1 knockdown significantly enhanced cell viability (Figure 4A) and inhibited the activity of LDH in the cellular supernatant (Figure 4B) under H/R injury."
39897566
reach
"The results showed that MYSM1 or EIF3H overexpression decreased cell viability, while PSMD14 overexpression increased cell viability under DOX treatment (Fig. 1C)."
39695708
MYSM1 affects CLOCK
| 2
MYSM1 binds CLOCK. 2 / 2
| 2
sparser
"When we used all 2364 arrays, strong positive correlation between two Myb-like transcription factor genes, Circadian Clock Associated 1 ( CCA1 ) and Late Elongated Hypocotyl ( LHY ) was observed, as well as weak negative correlation between Timing Of Cab expression 1 ( TOC1 ) and LHY , and between TOC1 and CCA1 (Fig.  xref A–C and Table  xref )."
18931094
sparser
"In Arabidopsis, the first-identified clock genes function in a double negative feedback loop, with two morning-phased Myb-like transcription factors, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), repressing expression of an evening-phased pseudo-response regulator, TIMING OF CAB EXPRESSION 1 (TOC1 or PRR1), which in turn represses expression of CCA1 and LHY ( xref ; xref ; xref ; xref ; xref ; xref ; xref )."
23638299
MYSM1 affects BBC3
| 1 1
MYSM1 inhibits BBC3.
| 1
MYSM1 inhibits BBC3. 1 / 1
| 1
reach
"Repression of p53-target gene Bbc3 and PUMA by MYSM1 is essential for the survival of hematopoietic multipotent progenitors and contributes to stem cell maintenance."
26768662
MYSM1 binds BBC3.
| 1
MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
| 1
sparser
"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
26768662
MYSM1 affects ARF
| 1 1
MYSM1 inhibits ARF.
| 1
MYSM1 inhibits ARF. 1 / 1
| 1
reach
"As Mysm1 generally functions as a transcription activator, it is rational to postulate that Mysm1 suppresses p53 or p19 ARF likely by activating expression of transcription repressors."
27277682
MYSM1 binds ARF.
| 1
TP53 binds ARF and MYSM1. 1 / 1
| 1
sparser
"In line with a previous report on Mysm1-binding to thep19 ARF -promoter in thymocytes [ xref ], an upstream regulator of TS p53, in Mysm1 −/− epidermal cells as well, mRNA expression ofp19 ARF was moderately increased along with increases in p53 levels and in apoptosis."
29495602
MYSM1 affects AMPK
| 2
MYSM1 inhibits AMPK. 2 / 2
| 2
reach
"These results suggested that the decrease of Mysm1 reduces the threshold of calcium mobilization and makes astrocytes easier to release more ATP.2.5 Mysm1 Knockdown Activated the p53/AMPK Pathway."
36414403
reach
"22 , 23 We set out to verify whether Mysm1 knockdown can activate the p53/AMPK pathway in primary astrocytes and C8‐D1A cells and then to evaluate the ATP level."
36414403
MPN domain affects TRIM21
| 2
MYSM1 binds TRIM21 and MPN domain. 2 / 2
| 2
reach
"MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner."
39695708
reach
"These data indicated that MYSM1 binds to TRIM21 via the SWIRM domain and deubiquitinates TRIM21 in an MPN domain-dependent manner (Fig. 6F)."
39695708
MET affects MYSM1
| 2
MYSM1 binds MET. 2 / 2
| 2
sparser
"In support of a collaboration of MYSM1 with PAX3 in transcriptional regulation, MYSM1 binding to the c-MET promoter in melanoma cells occurred in vicinity to the PAX3 consensus sequence previously described [ xref ]."
28978033
sparser
"In context with our finding that MYSM1 bound to the c-MET promoter region in close vicinity to PAX3 in melanoma cells, our data indicate that MYSM1 is an epigenetic regulator of melanoma growth and potentially promising new target for tumor therapy."
28978033
K63 affects STING1
| 2
MYSM1 binds STING1 and K63. 2 / 2
| 2
reach
"Activated MYSM1 binds to STING and removes its K63-linked polyubiquitination, repressing the synthesis of type I IFNs and pro-inflammatory cytokines, including IFN-β, IFN-stimulated gene 56 (ISG56), i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
reach
"MYSM1 binds to STING, removes its K63-linked ubiquitination, inhibits STING aggregation, and suppresses the formation of type I IFNs and pro-inflammatory cytokines, thereby restricting the cGAS-STING [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
ISD 45 affects MYSM1
| 2
ISD 45 activates MYSM1. 2 / 2
| 2
reach
"In addition, in C57BL/6 wild-type (WT) mouse embryonic fibroblasts (MEFs), Mysm1 mRNA was induced by HSV-1 ( Figure 1 C), poly(dA:dT), ISD 45, DNA 90, and HSV120 ( Figure 1 D)."
33086059
reach
"Similarly, in mouse bone marrow-derived macrophages (BMDMs), Mysm1 mRNA was activated by HSV-1 ( Figure 1 E), poly(dA:dT), ISD 45, DNA90, and HSV120 ( Figure 1 F), as well as by cGAMP ( Figure 1 G)."
33086059
IL3 affects MYSM1
| 1 1
MYSM1 binds IL3. 2 / 2
| 1 1
sparser
"MYSM1 interacts with nuclear factor IL-3 (NFIL3, also known as E4BP4), a critical factor for mouse NK cell development, and the recruitment of nuclear factor Il-3 to the ID2 locus is dependent on MYSM1."
24062447
reach
"MYSM1 interacts with nuclear factor IL-3 (NFIL3, also known as E4BP4), a critical factor for mouse NK cell development, and the recruitment of nuclear factor Il-3 to the ID2 locus is dependent on MYSM1."
24062447
HSV120 affects MYSM1
| 2
HSV120 activates MYSM1. 2 / 2
| 2
reach
"In addition, in C57BL/6 wild-type (WT) mouse embryonic fibroblasts (MEFs), Mysm1 mRNA was induced by HSV-1 ( Figure 1 C), poly(dA:dT), ISD 45, DNA 90, and HSV120 ( Figure 1 D)."
33086059
reach
"Similarly, in mouse bone marrow-derived macrophages (BMDMs), Mysm1 mRNA was activated by HSV-1 ( Figure 1 E), poly(dA:dT), ISD 45, DNA90, and HSV120 ( Figure 1 F), as well as by cGAMP ( Figure 1 G)."
33086059
H3C12 affects MYSM1
2 |
H3C12 binds MYSM1. 2 / 2
2 |
biogrid
No evidence text available
29568061
biogrid
No evidence text available
36089195
H2AX affects MYSM1
2 |
MYSM1 binds H2AX. 2 / 2
2 |
biogrid
No evidence text available
32466590
biogrid
No evidence text available
32466590
EP300 affects CREBBP
| 2
MYSM1 binds EP300 and CREBBP. 2 / 2
| 2
sparser
"2A-DUB associates with histone acetyltransferase p300/CBP-associated factor (PCAF/KAT2B) in a coregulatory protein complex and regulates transcription by coordinating histone acetylating, deubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18929679
sparser
"2A-DUB associates with the histone acetyltransferase p300/CBP-associated factor (p/CAF), a complex that mediates acetylation of histones H3 and H4 in transcriptionally active chromatin ( xref )."
19489724
DREB2A affects MYSM1
| 2
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
DREB2A binds MYSM1. 1 / 1
| 1
sparser
"We recently identified mediator in Arabidopsis thaliana and we have also reported that the Med25 subunit in Arabidopsis interacts with three different transcription factors, Dreb2a, Zfhd1 and myb-like which all are involved in different stress response pathways ( xref , xref ) In this article, we use a set of biochemical and biophysical methods to study interaction between the Med25 mediator subunit and the transcription factor Dreb2a from A. thaliana ."
22447446
DCT affects MYSM1
| 2
DCT activates MYSM1. 2 / 2
| 2
reach
"In addition, in C57BL/6 wild-type (WT) mouse embryonic fibroblasts (MEFs), Mysm1 mRNA was induced by HSV-1 ( Figure 1 C), poly(dA:dT), ISD 45, DNA 90, and HSV120 ( Figure 1 D)."
33086059
reach
"The results indicated that Mysm1 mRNA was induced by HSV-1 ( Figure 1 A) and poly(dA:dT) ( Figure 1 B) in human acute monocytic leukemia (THP-1) cell-derived macrophages."
33086059
CREBBP affects EP300, and MYSM1
| 2
MYSM1 binds EP300 and CREBBP. 2 / 2
| 2
sparser
"2A-DUB associates with histone acetyltransferase p300/CBP-associated factor (PCAF/KAT2B) in a coregulatory protein complex and regulates transcription by coordinating histone acetylating, deubiquitina[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18929679
sparser
"2A-DUB associates with the histone acetyltransferase p300/CBP-associated factor (p/CAF), a complex that mediates acetylation of histones H3 and H4 in transcriptionally active chromatin ( xref )."
19489724
CLOCK affects MYSM1
| 2
MYSM1 binds CLOCK. 2 / 2
| 2
sparser
"When we used all 2364 arrays, strong positive correlation between two Myb-like transcription factor genes, Circadian Clock Associated 1 ( CCA1 ) and Late Elongated Hypocotyl ( LHY ) was observed, as well as weak negative correlation between Timing Of Cab expression 1 ( TOC1 ) and LHY , and between TOC1 and CCA1 (Fig.  xref A–C and Table  xref )."
18931094
sparser
"In Arabidopsis, the first-identified clock genes function in a double negative feedback loop, with two morning-phased Myb-like transcription factors, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY), repressing expression of an evening-phased pseudo-response regulator, TIMING OF CAB EXPRESSION 1 (TOC1 or PRR1), which in turn represses expression of CCA1 and LHY ( xref ; xref ; xref ; xref ; xref ; xref ; xref )."
23638299
ATM affects MYSM1
| 1 1
ATM phosphorylates MYSM1. 2 / 2
| 1 1
sparser
"MYSM1 is phosphorylated by ATM in response to DNA injury. xref It is conceivable that this modification alters MYSM1 activity or recruitment to chromatin at DNA damage."
38065233
reach
"MYSM1 is phosphorylated by ATM in response to DNA injury."
38065233
Valproic acid affects MYSM1
1 |
Valproic acid decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
23179753
Tungsten affects MYSM1
1 |
Tungsten decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
30912803
Troglitazone affects MYSM1
1 |
Troglitazone decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
28973697
Transferase affects MYSM1
| 1
MYSM1 binds transferase. 1 / 1
| 1
sparser
"2A-DUB is associated with the p/CAF histone acetyl transferase."
18430235
reach
"As shown in Figure S1C-D, both mRNA and protein levels of MYSM1 were elevated in HL-1 cells overexpressing CEBPB, suggesting that CEBPB may function as a cardiac transcription regulator promoting MYSM1's transcriptional activity."
39897566
Titanium dioxide affects MYSM1
1 |
Titanium dioxide demethylates MYSM1. 1 / 1
1 |
ctd
No evidence text available
35295148
Thioacetamide affects MYSM1
1 |
Thioacetamide increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
34492290
Thapsigargin affects MYSM1
1 |
Thapsigargin increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
24648495
Testosterone undecanoate decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
19074003
Sunitinib affects MYSM1
1 |
Sunitinib increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
31533062
Sulforaphane affects MYSM1
1 |
Sulforaphane decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
30529165
Sulfadimethoxine affects MYSM1
1 |
Sulfadimethoxine decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
30047161
Sorafenib affects MYSM1
1 |
Sorafenib increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
39786591
Sodium arsenate affects MYSM1
1 |
Sodium arsenate increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
21795629
Response to hypoxia decreases the amount of MYSM1. 1 / 1
1 |
bel
"Upon hypoxia, the expression of HIF-1alpha and VEGF gene was induced. The result of Western blotting showed that the expression of alpha-SMA was increased by hypoxic stimulation. Furthermore, the expression of MMP-2 and TIMP-1 genes was increased. Hypoxia also elevated the protein expression of the collagen type I in LX-2 cells. The analysis of TGF-beta/Smad signaling pathway showed that hypoxia potentiated the expression of TGF-beta1 and the phosphorylation status of Smad2. See Table 2 for results of microarray experiment."
17187782
Propiconazole affects MYSM1
1 |
Propiconazole decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
21278054
Progesterone affects MYSM1
1 |
Progesterone decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
19690047
Polyubiquitin affects MYSM1
| 1
Polyubiquitin binds MYSM1. 1 / 1
| 1
sparser
"Denaturation effectively disrupted the association of MYSM1 with TRAF3, TRAF6, pCAF, or K63 polyubiquitins."
26474655
P53-target gene promoters affects MYSM1
| 1
MYSM1 binds p53-target gene promoters. 1 / 1
| 1
reach
"The recruitment of MYSM1 to p53-target gene promoters was tested using chromatin immunoprecipitation (ChIP) in a Ba/F3 hematopoietic progenitor cell line stably expressing FLAG-MYSM1 (XREF_SUPPLEMENTARY)."
26768662
P16 INK4a mRNA affects MYSM1
| 1
P16 INK4a mRNA activates MYSM1. 1 / 1
| 1
reach
"In contrast to p19 ARF, p16 INK4a mRNA was only slightly induced in Mysm1 deficient thymocytes (XREF_FIG (left) and XREF_FIG)."
25613381
Methimazole affects MYSM1
1 |
Methimazole decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
30047161
Levonorgestrel affects MYSM1
1 |
Levonorgestrel decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
19074003
Imatinib affects MYSM1
| 1
Imatinib inhibits MYSM1. 1 / 1
| 1
reach
"As shown in Fig. 5I, Imatinib diminished the activity of MYSM1 as an ERα co-activator in a dose-dependent manner in MCF-7 cells."
38177530
Hsa-miR-376b-3p affects MYSM1
1 |
Hsa-miR-376b-3p decreases the amount of MYSM1. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23824327
Hsa-miR-186-5p affects MYSM1
1 |
Hsa-miR-186-5p decreases the amount of MYSM1. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23622248
Formaldehyde affects MYSM1
1 |
Formaldehyde increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
20655997
Endosulfan affects MYSM1
1 |
Endosulfan increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
26615145
Doxorubicin affects MYSM1
1 |
Doxorubicin decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
29803840
Dorsomorphin affects MYSM1
| 1
Dorsomorphin activates MYSM1. 1 / 1
| 1
reach
"Pifithrin alpha (PFT alpha) and Compound C, antagonists of p53 and AMPK, respectively, repressed ATP production and reversed the antidepressant effect of Mysm1 knockdown."
36414403
Dichloromethane affects MYSM1
1 |
Dichloromethane decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
28392392
Diazinon affects MYSM1
1 |
Diazinon increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
29108742
Chlorpyrifos affects MYSM1
1 |
Chlorpyrifos decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
37019170
Cadmium dichloride increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
38568856
C-MET promoter affects MYSM1
| 1
MYSM1 binds c-MET promoter. 1 / 1
| 1
reach
"In support of a collaboration of MYSM1 with PAX3 in transcriptional regulation, MYSM1 binding to the c-MET promoter in melanoma cells occurred in vicinity to the PAX3 consensus sequence previously described [XREF_BIBR]."
28978033
C-MET promoter region affects MYSM1
| 1
MYSM1 binds c-MET promoter region. 1 / 1
| 1
reach
"In context with our finding that MYSM1 bound to the c-MET promoter region in close vicinity to PAX3 in melanoma cells, our data indicate that MYSM1 is an epigenetic regulator of melanoma growth and potentially promising new target for tumor therapy."
28978033
Amitrole affects MYSM1
1 |
Amitrole decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
30047161
Abrine affects MYSM1
1 |
Abrine increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
31054353
ZNF330 affects MYSM1
1 |
ZNF330 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
XIAP affects RIPK2
| 1
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
Vincristine affects MYSM1
1 |
Vincristine increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
23649840
Vesicular Stomatitis inhibits MYSM1. 1 / 1
| 1
reach
"), a bacterial pathogen that activates multiple PRRs including TLRs, RLRs, and CDRs, or Vesicular stomatitis virus (VSV), which predominantly activates RLRs, Mysm1 shRNA-silenced RAW 264.7 macrophages[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26474655
USP22 affects TMPRSS11D
| 1
MYSM1 binds USP22 and TMPRSS11D. 1 / 1
| 1
sparser
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
UBTF affects MYSM1
1 |
MYSM1 binds UBTF. 1 / 1
1 |
biogrid
No evidence text available
32466590
UBC affects MYSM1
1 |
MYSM1 binds UBC. 1 / 1
1 |
biogrid
No evidence text available
17707232
TRF2 affects MYSM1
| 1
TRF2 binds MYSM1. 1 / 1
| 1
sparser
"TRF2 associates with telomeric DNA via a myb-like DNA binding domain."
19854131
TRAF affects MYSM1
| 1
TRAF binds MYSM1. 1 / 1
| 1
sparser
"This observation aligns with prior reports showing that the SWIRM domain mediates MYSM1’s interaction with TRAF family proteins, including the TRAF3/6 complex xref ."
41162619
TP63 affects MYSM1
| 1
TP63 increases the amount of MYSM1. 1 / 1
| 1
reach
"Alternatively, Mysm1 may influence p63 transcription by counteracting chromatin changes induced by PRC1 component Bmi1 -- with context dependent activatory or inhibitory influence in stem cells [XREF_BIBR], [and unpublished meeting communications]."
29495602
TP53 affects CDKN1A
| 1
MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
| 1
sparser
"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
26768662
TP53 affects ARF
| 1
TP53 binds ARF and MYSM1. 1 / 1
| 1
sparser
"In line with a previous report on Mysm1-binding to thep19 ARF -promoter in thymocytes [ xref ], an upstream regulator of TS p53, in Mysm1 −/− epidermal cells as well, mRNA expression ofp19 ARF was moderately increased along with increases in p53 levels and in apoptosis."
29495602
TNFAIP3 affects RNF31
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
TNC affects MYSM1
| 1
TNC activates MYSM1. 1 / 1
| 1
reach
"36 Belle JI, Langlais D, Petrov JC, Pardo M, Jones RG, Gros P, et al. p53 mediates loss of hematopoietic stem cell function and lymphopenia in Mysm1 deficiency."
26923762
TMPRSS11D affects USP22
| 1
MYSM1 binds USP22 and TMPRSS11D. 1 / 1
| 1
sparser
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
THR affects MYSM1
| 1
MYSM1 binds THR. 1 / 1
| 1
reach
"Histone H2A deubiquitinase (2A-DUB) forms a complex with approximately 10 proteins such as histone acetyltransferase p300/CBP-associated factor (p/CAF; presently, PCAF), thyroid hormone receptor-interacting protein 5 (Trip5) and RBM10 (Zhu et al., 2007)."
33515724
TERF2IP affects MYSM1
1 |
TERF2IP binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
TEAD4 affects MYSM1
1 |
MYSM1 binds TEAD4. 1 / 1
1 |
biogrid
No evidence text available
38594215
TCEAL9 affects MYSM1
1 |
TCEAL9 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
33961781
TCEA1 affects MYSM1
| 1
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
TACSTD2 affects MYSM1
| 1
MYSM1 binds TACSTD2. 1 / 1
| 1
sparser
"TACSTD2-MYSM1, LRP1B, and ASAP1-ADCY8 showed suggestive associations with serum Fe element levels (p < 5 × 10 -6 )."
34417961
TAB3 affects TAB2
| 1
TAB3 binds TAB2, MYSM1, and RNF31. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
T-2 toxin affects MYSM1
1 |
T-2 toxin increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
36695871
STING1 affects MPN domain
| 1
MYSM1 binds STING1 and MPN domain. 1 / 1
| 1
reach
"We have shown that the SWIRM domain is necessary for MYSM1 interaction with STING, and the MPN domain is necessary for STING deubiquitination.In addition to the defense against viral infections, STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
STAT1 affects MPN domain.According
| 1
MYSM1 binds STAT1 and MPN domain.According. 1 / 1
| 1
reach
"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain.According to our ubiquitinomics, the K379 residue of STAT1, located in the DNA binding domain (DBD) of STAT1 (Figure 6K), was the most significantly reduced deubiquitination residue of STAT1 after MYSM1 overexpression (Figure 5E)."
39897566
SNRNP40 affects MYSM1
1 |
SNRNP40 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37689310
SIRT1 affects MYSM1
| 1
SIRT1 inhibits MYSM1. 1 / 1
| 1
reach
"The expression of PGC1α and Sirt1 increased consistently with decreased Mysm1."
36414403
reach
"Deubiquitinase MYSM1 drives myocardial ischemia/reperfusion injury by stabilizing STAT1 in cardiomyocytes."
39897566
RT-qPCR affects MYSM1
| 1
RT-qPCR increases the amount of MYSM1. 1 / 1
| 1
reach
"RT-qPCR further showed that DOX significantly increased the mRNA levels of Mysm1 in cardiomyocytes rather than non-cardiomyocytes (Fig. 1J)."
39695708
RPL10 affects MYSM1
| 1
RPL10 binds MYSM1. 1 / 1
| 1
sparser
"Furthermore, upon relocalization, RPL10 interacts with the MYB-like protein L10-interacting myb domain-containing protein (LIMYB) to suppress the expression of translational machinery genes, which results in enhanced tolerance to begomovirus infection [ xref ]."
30403659
RNF31 affects TNFAIP3
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
RNF31 affects TAB3
| 1
TAB3 binds TAB2, MYSM1, and RNF31. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
RNF31 affects OTULIN
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
RIPK2 affects XIAP
| 1
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
RFC5 affects PCNA
| 1
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
RFC4 affects MYSM1
1 |
MYSM1 binds RFC4. 1 / 1
1 |
biogrid
No evidence text available
32466590
RAS affects MYSM1
| 1
RAS binds MYSM1. 1 / 1
| 1
sparser
"We found that certain domains, including Myb-like DNA-binding, Ras, kinase, DnaJ, and domains commonly associated with metabolic enzymes, were significantly enriched (Additional file xref : Fig. S15C)."
37024975
RAB5B affects MYSM1
1 |
MYSM1 binds RAB5B. 1 / 1
1 |
biogrid
No evidence text available
40437099
Phosphatase affects MYSM1
| 1
sparser
"In Arabidopsis, for instance, InsP 8 binding to SPX receptors promotes association of the PP-InsP/SPX complex with MYB-like PHR transcription factors ( xref ; xref ; xref ), known master regulators of phosphate starvation responses (PSRs) ( xref )."
38455731
Petrov JC affects MYSM1
| 1
Petrov JC activates MYSM1. 1 / 1
| 1
reach
"36 Belle JI, Langlais D, Petrov JC, Pardo M, Jones RG, Gros P, et al. p53 mediates loss of hematopoietic stem cell function and lymphopenia in Mysm1 deficiency."
26923762
PROMO database affects MYSM1
| 1
PROMO database activates MYSM1. 1 / 1
| 1
reach
"Further, we investigated the transcriptional activation of MYSM1 by PROMO database (Table S4) and CHIP atlas database (Table S5)."
39897566
PRMT5 affects MYSM1
1 |
MYSM1 binds PRMT5. 1 / 1
1 |
biogrid
No evidence text available
17707232
PPIB affects MYSM1
1 |
MYSM1 binds PPIB. 1 / 1
1 |
biogrid
No evidence text available
32466590
PPARGC1B affects MYSM1
| 1
PPARGC1B binds MYSM1. 1 / 1
| 1
sparser
"Proteins were transferred onto PVDF (polyvinylidene difluoride) membranes in cold buffer (25 m m Tris Base, 192 m m glycine) by electrotransfer for 1.5 h, which were incubated in TBS buffer containing 5% milk for 1 h at 22–24 °C. The membrane was probed at 4 °C overnight with the following primary antibodies: Rabbit anti‐Mysm1 (1:1000, Abcam), rabbit anti‐PGC1 α (1:1000, Cell Signaling Technology), rabbit anti‐p‐p53 (1:1000, Cell Signaling Technology), rabbit anti‐p53 (1:1000, Cell Signaling Technology), rabbit anti‐p‐AMPK (1:1000, Cell Signaling Technology), rabbit anti‐AMPK (1:1000, Cell Signaling Technology), rabbit anti‐Sirt1 (1:1000, Cell Signaling Technology), rabbit anti‐p‐mTOR (1:1000, Cell Signaling Technology), rabbit anti‐mTOR(1:1000, Cell Signaling Technology), rabbit anti‐p‐S6 (1:1000, Cell Signaling Technology), mouse anti‐S6 (1:1000, Cell Signaling Technology), rabbit anti‐GAPDH (1:1000, Abclonal), mouse anti‐ β ‐actin (1:1000, APPLYGEN or Cell Signaling Technology)."
36414403
POLD1 affects MYSM1
| 1
POLD1 binds MSH6 and MYSM1. 1 / 1
| 1
sparser
"Furthermore, mild interactions of MYSM1 with POLD1 and MSH6 and with Lamin B1, involved in higher-order chromatin structure and anchoring of chromatin, were detectable."
32466590
PHLPP1 affects MYSM1
| 1
PHLPP1 binds MYSM1. 1 / 1
| 1
sparser
"The qRT-PCR analyses showed that siRNA-mediated downregulation of MYSM1 resulted in a significant decrease in PHLPP1 expression, confirming the association of MYSM1 with PHLPP1 ( xref )."
31761786
PCNA affects RFC5
| 1
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
PAX5 affects MYSM1
| 1
PAX5 increases the amount of MYSM1. 1 / 1
| 1
reach
"Mechanistic studies revealed that MYSM1 activates Pax5 transcription by coordinating histone modifications and directing the recruitment of the transcription activators such as PU.1 to the Pax5 locus in B cells."
26348977
PAC affects MYSM1
| 1
MYSM1 binds HTH and PAC. 1 / 1
| 1
sparser
"An unexpectedly potent size regulator that emerged from our screens was Dot6, a Myb-like HTH transcription factor that binds to the PAC (Polymerase A and C) motif [ xref ]."
30921326
P6.9 affects MYSM1
| 1
P6.9 binds MYSM1. 1 / 1
| 1
sparser
"Telomeric DNA is assembled into a non nucleosomal structure, whose main component is Rap1p, a duplex, sequence specific DNA binding protein that binds telomeric DNA with its Myb-like binding domain an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19007917
Osteoarthritis affects MYSM1
| 1
reach
"The possible association between MYSM1 and OA was further examined in vivo."
39746943
OTULIN affects TNFAIP3
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
O41964 affects MYSM1
1 |
MYSM1 binds O41964. 1 / 1
1 |
virhostnet
No evidence text available
22028648
Nanotubes, Carbon affects MYSM1
1 |
Nanotubes, Carbon decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
25554681
NUP43 affects MYSM1
1 |
NUP43 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37689310
NOD2 affects XIAP
| 1
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
Myocardial Ischemia activates MYSM1. 1 / 1
| 1
reach
"Deubiquitinase MYSM1 drives myocardial ischemia/reperfusion injury by stabilizing STAT1 in cardiomyocytes."
39897566
MiR-129-5p affects MYSM1
| 1
MiR-129-5p binds MYSM1. 1 / 1
| 1
sparser
"We further utilized a luciferase reporter assay to validate the direct interaction between miR-129-5p and Mysm1."
40344568
MYSM1 affects ubiquitination
| 1
MYSM1 inhibits ubiquitination. 1 / 1
| 1
sparser
"The deubiquitinases OTUB1/2 [ xref , xref ], UCHL1 [ xref ], MYSM1 [ xref ] and DUBA [ xref ] inhibit K63-linked ubiquitination of TRAF3 or TRAF6 and negatively regulate IFNs production during viral infection."
29734366
MYSM1 affects transferase
| 1
MYSM1 binds transferase. 1 / 1
| 1
sparser
"2A-DUB is associated with the p/CAF histone acetyl transferase."
18430235
MYSM1 increases the amount of transcriptional regulator. 1 / 1
| 1
reach
"In early B cells, MYSM1 promotes expression of Ebf1, a critical transcriptional regulator of B-cell differentiation."
38065233
MYSM1 affects tissue function
| 1
MYSM1 activates tissue function. 1 / 1
| 1
eidos
"MYSM1 restricts the Rb and p53 pathways in senescent cells and mouse tissues , improves tissue function , and reduces the accumulation of aged cells ."
33240758
MYSM1 affects tamoxifen
| 1
MYSM1 inhibits tamoxifen. 1 / 1
| 1
reach
"Compared with the control group, MYSM1-depleted TMR cells exhibited restored Tamoxifen sensitivity (Figs."
38177530
MYSM1 affects senescence cell accumulation mice tissues
| 1
MYSM1 inhibits senescence cell accumulation mice tissues. 1 / 1
| 1
eidos
"These findings indicated that loss of MYSM1 promotes senescence cell accumulation in mice tissues ."
33240758
MYSM1 inhibits response to stress. 1 / 1
| 1
reach
"53 Our work shows that MYSM1 represses p53 stress response genes by antagonizing other histone modifications (H3K27ac and H3K4me3)."
26768662
MYSM1 inhibits regulation of mitochondrial membrane potential. 1 / 1
| 1
reach
"The Mysm1 -/- thymocytes also produced enhanced levels of reactive oxygen species (ROS), but reduced the mitochondrial membrane potential (XREF_FIG)."
27277682
MYSM1 inhibits regulation of cell cycle. 1 / 1
| 1
reach
"MYSM1-deficient cells have increased p38 activation, cell cycle arrest, and increased apoptosis, presumably due to altered transcriptional activity."
29731008
MYSM1 affects recruitment hematopoietic transcription PU.1
| 1
MYSM1 activates recruitment hematopoietic transcription PU.1. 1 / 1
| 1
eidos
"MYSM1 bound to the Flt3 gene promoter , and loss of MYSM1 resulted in reduced recruitment of the major hematopoietic transcription factor PU.1 and an increase in repressive histone marks H2AK119ub and H3K27me3 at the Flt3 locus [ 33 ] ( Figure 2 ) ."
32344625
MYSM1 affects recruitment PRC1 complex proteins RING1B
| 1
MYSM1 inhibits recruitment PRC1 complex proteins RING1B. 1 / 1
| 1
eidos
"MYSM1 was required for normal recruitment of transcription factors GATA2 and RUNX1 to the Gfi1 locus , while loss of MYSM1 resulted in increased recruitment of the PRC1 complex proteins RING1B and BMI1 and increased levels of histone H2AK119ub [ 34 ] ( Figure 2 ) ."
32344625
MYSM1 affects recruitment E4BP4 Id2 locus
| 1
MYSM1 inhibits recruitment E4BP4 Id2 locus. 1 / 1
| 1
eidos
"In support of the role of ID2 in NK cell maturation , deficiency of MYSM1 , which mediates the recruitment of E4BP4 to the Id2 locus , leads to defects in NK cell maturation , but not NK lineage specification or commitment ( 23 ) ."
32849653
MYSM1 affects processes senescence
| 1
MYSM1 inhibits processes senescence. 1 / 1
| 1
eidos
"MYSM1 represses the processes of senescence and the progress of aging , prevents the damage to vital organs , and reduces the development of aging-associated diseases , which lead to the prolongation of lifespan ."
33240758
MYSM1 affects potential primary osteoblasts differentiate mature osteoblasts
| 1
MYSM1 activates potential primary osteoblasts differentiate mature osteoblasts. 1 / 1
| 1
eidos
"Recently , study demonstrates that MYSM1 deficiency impairs the potential for primary osteoblasts to differentiate into mature osteoblasts ."
30123285
MYSM1 affects polyubiquitin
| 1
Polyubiquitin binds MYSM1. 1 / 1
| 1
sparser
"Denaturation effectively disrupted the association of MYSM1 with TRAF3, TRAF6, pCAF, or K63 polyubiquitins."
26474655
eidos
"Altogether , MYSM1 was proposed to inhibit plasma cell differentiation by maintaining Pax5 expression , via histone H2AK119-deubiquitination and recruitment of transcription factor PU.1 to the Pax5 locus [ 30 ] ( Figure 2 ) ."
32344625
MYSM1 affects pathways
| 1
MYSM1 inhibits pathways. 1 / 1
| 1
sparser
"MYSM1 inhibits the pathogen recognition receptor pathways for pro‐inflammatory and type I interferon gene induction, acting as a key regulator of innate immunity [ xref , xref ]."
37601868
MYSM1 affects pathogenesis
| 1
MYSM1 inhibits pathogenesis. 1 / 1
| 1
reach
"These results underscore that reduced MYSM1 levels accelerate cartilage degeneration.Inflammatory responses play a critical role in OA pathogenesis."
39746943
MYSM1 affects pathogen recognition receptor
| 1
MYSM1 inhibits pathogen recognition receptor. 1 / 1
| 1
reach
"MYSM1 inhibits the pathogen recognition receptor pathways for pro‐inflammatory and type I interferon gene induction, acting as a key regulator of innate immunity [10, 11]."
37601868
MYSM1 affects pS176
| 1
MYSM1 decreases the amount of pS176. 1 / 1
| 1
reach
"Although MYSM1 inhibits the pS176 level of RIPK2, it rapidly increased after exposure to Okadaic Acid (Fig. 6l, m)."
39746943
MYSM1 affects p53-target gene promoters
| 1
MYSM1 binds p53-target gene promoters. 1 / 1
| 1
reach
"The recruitment of MYSM1 to p53-target gene promoters was tested using chromatin immunoprecipitation (ChIP) in a Ba/F3 hematopoietic progenitor cell line stably expressing FLAG-MYSM1 (XREF_SUPPLEMENTARY)."
26768662
MYSM1 affects p53-activation
| 1
MYSM1 inhibits p53-activation. 1 / 1
| 1
eidos
"This was also observed after an inducible deletion of Mysm1 in adult mice , indicating that MYSM1 is required constitutively to repress p53-activation [ 46 ] ."
32344625
MYSM1 affects p53 pathways
| 1
MYSM1 inhibits p53 pathways. 1 / 1
| 1
eidos
"MYSM1 restricts the Rb and p53 pathways in senescent cells and mouse tissues , improves tissue function , and reduces the accumulation of aged cells ."
33240758
reach
"These results suggest that Mysm1 knockdown in astrocytes may upregulate TCA cycle process and mitochondrial oxidative phosphorylation levels to enhance mitochondrial function.Combined with the results that Mysm1 knockdown in astrocytes in vitro could produce more ATP, we explored whether Mysm1 knockdown in vivo could increase ATP in the interstitial space of the brain."
36414403
MYSM1 affects organs
| 1
MYSM1 inhibits organs. 1 / 1
| 1
eidos
"MYSM1 represses the processes of senescence and the progress of aging , prevents the damage to vital organs , and reduces the development of aging-associated diseases , which lead to the prolongation of lifespan ."
33240758
MYSM1 affects neurogenesis
| 1
MYSM1 inhibits neurogenesis. 1 / 1
| 1
reach
"The data revealed that Mysm1-deficient NSCs exhibited hyperproliferation, increased apoptosis, enhanced neurogenesis, and compromised astrogliogenesis."
38342917
MYSM1 affects miR-200 family members
| 1
MYSM1 inhibits miR-200 family members. 1 / 1
| 1
reach
"MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling."
34706761
MYSM1 affects miR-150 transcription
| 1
MYSM1 activates miR-150 transcription. 1 / 1
| 1
eidos
"Mysm1 , a histone deubiquitinase , is induced by TNF-alpha and IFNgamma in ASCs and promotes miR-150 transcription , which enhances iNOS production ."
32363193
MYSM1 affects miR-129-5p
| 1
MiR-129-5p binds MYSM1. 1 / 1
| 1
reach
"We further utilized a luciferase reporter assay to validate the direct interaction between miR-129-5p and Mysm1."
40344568
MYSM1 inhibits inflammatory response. 1 / 1
| 1
reach
"MYSM1 deficiency leads to heightened inflammation characterized by increased cytokine production."
39746943
MYSM1 affects immune response
| 1
MYSM1 activates immune response. 1 / 1
| 1
reach
"Tatsuya et al. suggested that MYSM1 heterozygous defects lead to an excessive immune response to exogenous antigens, increasing susceptibility to acquired AA."
38988263
MYSM1 activates homeostatic process. 1 / 1
| 1
reach
"Strong IL-15 receptor stimulation by IL-2 pre-ligated to an anti-IL-2 antibody, or SOCS-3 deficiency, partially restores homeostasis in Id2 deficient NK cells.In support of the role of ID2 in NK cell maturation, deficiency of MYSM1, which mediates the recruitment of E4BP4 to the Id2 locus, leads to defects in NK cell maturation, but not NK lineage specification or commitment (23)."
32849653
MYSM1 activates fat cell differentiation. 1 / 1
| 1
reach
"In addition to regulating HSCs, Mysm1 also plays a critical role in mesenchymal stem cell (MSC) maintenance and differentiation, and Mysm1 knockout MSCs show enhanced autonomous differentiation and accelerated adipogenesis [21]."
38342917
MYSM1 affects etoposide
| 1
MYSM1 activates etoposide. 1 / 1
| 1
reach
"Nuclear MYSM1 intensity increased as early as 0.5 h post etoposide exposure of KG-1a cells, and stayed elevated for 1–3 h in parallel with increases in γH2AX cells as indicated in the quantifications (Figure 1D)."
32466590
MYSM1 inhibits epithelial to mesenchymal transition. 1 / 1
| 1
reach
"Furthermore, silencing MYSM1 stimulated PI3K/AKT signaling and promoted EMT in CRC cells."
34706761
MYSM1 affects doxorubicin
| 1
MYSM1 activates doxorubicin. 1 / 1
| 1
reach
"As a deubiquitinating enzyme, mysm1 regulates trim 21 stability and mediates doxorubicin (DOX) - induced ferroptosis in cardiomyocytes, suggesting that cardiomyocyte specific knockdown of mysm1 may be an attractive therapeutic strategy for this disease (82)."
40190567
MYSM1 affects dioxygen
| 1
MYSM1 activates dioxygen. 1 / 1
| 1
reach
"The Mysm1 -/- thymocytes also produced enhanced levels of reactive oxygen species (ROS), but reduced the mitochondrial membrane potential (XREF_FIG)."
27277682
MYSM1 affects development aging-associated diseases
| 1
MYSM1 inhibits development aging-associated diseases. 1 / 1
| 1
eidos
"MYSM1 represses the processes of senescence and the progress of aging , prevents the damage to vital organs , and reduces the development of aging-associated diseases , which lead to the prolongation of lifespan ."
33240758
MYSM1 affects defects
| 1
MYSM1 inhibits defects. 1 / 1
| 1
eidos
"Several studies have found that MYSM1 deficiency or mutation can lead to defects during the development and function of HSCs , B cells , natural killer ( NK ) cells and dendritic cells , and it also results in the development of lymphopenia , anemia , and thrombocytopenia , and low B-cell and NK-cell counts in both mice and humans [ 45,51,52,53,54,55 ] ."
32354135
MYSM1 affects defects NK cell maturation
| 1
MYSM1 inhibits defects NK cell maturation. 1 / 1
| 1
eidos
"In support of the role of ID2 in NK cell maturation , deficiency of MYSM1 , which mediates the recruitment of E4BP4 to the Id2 locus , leads to defects in NK cell maturation , but not NK lineage specification or commitment ( 23 ) ."
32849653
MYSM1 activates decreased bone mass. 1 / 1
| 1
eidos
"MYSM1 deficiency leads to decreased bone mass ."
30123285
MYSM1 affects colony
| 1
MYSM1 activates colony. 1 / 1
| 1
eidos
"These findings indicated that MYSM1 promotes cell proliferation and colony formation and attenuates SA-beta-Gal activation to repress senescence ."
33240758
MYSM1 affects c-MET promoter
| 1
MYSM1 binds c-MET promoter. 1 / 1
| 1
reach
"In support of a collaboration of MYSM1 with PAX3 in transcriptional regulation, MYSM1 binding to the c-MET promoter in melanoma cells occurred in vicinity to the PAX3 consensus sequence previously described [XREF_BIBR]."
28978033
MYSM1 affects c-MET promoter region
| 1
MYSM1 binds c-MET promoter region. 1 / 1
| 1
reach
"In context with our finding that MYSM1 bound to the c-MET promoter region in close vicinity to PAX3 in melanoma cells, our data indicate that MYSM1 is an epigenetic regulator of melanoma growth and potentially promising new target for tumor therapy."
28978033
MYSM1 inhibits biosynthetic process. 1 / 1
| 1
reach
"Importantly, the treatment with MYSM1 represses the synthesis of type I IFNs and pro-inflammatory cytokines in the PBMCs of SLE patients.Therefore, MYSM1 plays a pivotal role in the restriction of inn[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects binding PRC1 complex proteins
| 1
MYSM1 inhibits binding PRC1 complex proteins. 1 / 1
| 1
eidos
"At many of these loci , loss of MYSM1 resulted in increased binding of PRC1 complex proteins and elevated levels of histone H2AK119ub [ 20,30,32,33,34 ] , suggesting that MYSM1 antagonizes PRC1-mediated histone ubiquitination and transcriptional repression ."
32344625
MYSM1 affects aging-related pathologies
| 1
MYSM1 inhibits aging-related pathologies. 1 / 1
| 1
eidos
"Together , the findings demonstrated that Mysm1 deficiency promotes aging-related pathologies , facilitates developmental defects and early death , thereby shortening the lifespan of mice ."
33240758
MYSM1 affects aging aging-related pathologies
| 1
MYSM1 inhibits aging aging-related pathologies. 1 / 1
| 1
eidos
"Mysm1 deficiency promotes aging and aging-related pathologies and reduces lifespan in mice ."
33240758
MYSM1 affects activation
| 1
MYSM1 inhibits activation. 1 / 1
| 1
sparser
"MYSM1 interacts with AR and inhibits activation of Akt/c-Raf/GSK-3β signaling in prostate cancer."
31761786
MYSM1 affects activation p53 stress response
| 1
MYSM1 inhibits activation p53 stress response. 1 / 1
| 1
eidos
"] Loss of Mysm1 has been shown to promote activation of the p53 stress response and induced abnormal cell development and tissue differentiation ."
33240758
MYSM1 affects accumulation aged cells
| 1
MYSM1 inhibits accumulation aged cells. 1 / 1
| 1
eidos
"MYSM1 restricts the Rb and p53 pathways in senescent cells and mouse tissues , improves tissue function , and reduces the accumulation of aged cells ."
33240758
MYSM1 affects ZNF330
1 |
ZNF330 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
MYSM1 affects XIAP
| 1
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 affects USP22
| 1
MYSM1 binds USP22 and TMPRSS11D. 1 / 1
| 1
sparser
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
MYSM1 affects UBTF
1 |
MYSM1 binds UBTF. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects UBC
1 |
MYSM1 binds UBC. 1 / 1
1 |
biogrid
No evidence text available
17707232
MYSM1 affects TRF2
| 1
TRF2 binds MYSM1. 1 / 1
| 1
sparser
"TRF2 associates with telomeric DNA via a myb-like DNA binding domain."
19854131
MYSM1 affects TRAF
| 1
TRAF binds MYSM1. 1 / 1
| 1
sparser
"This observation aligns with prior reports showing that the SWIRM domain mediates MYSM1’s interaction with TRAF family proteins, including the TRAF3/6 complex xref ."
41162619
MYSM1 affects TP53BP1
| 1
MYSM1 inhibits TP53BP1. 1 / 1
| 1
reach
"We find that loss of MYSM1 leads to prolonged retention of 53BP1 at DSBs and continued DDR signaling, supporting that MYSM1 functions in termination of DDR signaling."
38065233
MYSM1 affects TNFAIP3
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
MYSM1 affects TMPRSS11D, and USP22
| 1
MYSM1 binds USP22 and TMPRSS11D. 1 / 1
| 1
sparser
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
MYSM1 affects THY1
| 1
MYSM1 activates THY1. 1 / 1
| 1
reach
"Though iron transport, storage, and metabolism as well as antioxidant genes were transcriptionally upregulated or remained unchanged, the levels of key proteins including GPX4, SLC7A11, and FTH1 were all significantly reduced in HSPCs and most profoundly reduced in the primitive HSC-enriched CD34 CD45RA CD90 compartment, which could be rescued by re-expressing WT MYSM1, but not the disease-associated loss-of-function mutants (Figure 6A–B, S6A–B)."
36803603
MYSM1 affects TFS1
| 1
MYSM1 activates TFS1. 1 / 1
| 1
reach
"With the extension of the chronic restraint time, the expression of Mysm1 gradually increased in the MHb, HIP, and IC (Figure 1E)."
36414403
MYSM1 affects TEWL
| 1
MYSM1 activates TEWL. 1 / 1
| 1
reach
"Mysm1 deficient mice had significantly increased TEWL compared with their wild-type littermates (XREF_FIG B); reflecting potential changes in epidermal barrier formation at this age group."
29495602
MYSM1 affects TERF2IP
1 |
TERF2IP binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
MYSM1 affects TEAD4
1 |
MYSM1 binds TEAD4. 1 / 1
1 |
biogrid
No evidence text available
38594215
MYSM1 affects TCEAL9
1 |
TCEAL9 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
33961781
MYSM1 affects TCEA1
| 1
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
MYSM1 affects TACSTD2
| 1
MYSM1 binds TACSTD2. 1 / 1
| 1
sparser
"TACSTD2-MYSM1, LRP1B, and ASAP1-ADCY8 showed suggestive associations with serum Fe element levels (p < 5 × 10 -6 )."
34417961
MYSM1 affects TAB3
| 1
TAB3 binds TAB2, MYSM1, and RNF31. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 affects STAT1.The JAMMs family
| 1
MYSM1 activates STAT1.The JAMMs family. 1 / 1
| 1
reach
"Subsequently, MYSM1 initiated the expression of necroptosis-related genes by promoting the transcriptional activity of STAT1.The JAMMs family represents the sole zinc metalloprotease family, while the remaining DUB families consist of cysteine peptidases 10."
39897566
MYSM1 affects SNRNP40
1 |
SNRNP40 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37689310
MYSM1 affects SLC7A11
| 1
MYSM1 activates SLC7A11. 1 / 1
| 1
reach
"Indeed, the latest evidence suggests that human cord blood HSCs are more susceptible to ferroptosis than downstream progenitor cells ex vivo due to a low level of antioxidant protein synthesis, and MY[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
38582294
MYSM1 activates Reperfusion Injury. 1 / 1
| 1
reach
"Next, we verified the elevated mRNA levels of MYSM1 in H/R-induced HL-1 cells (Figure 1E) and myocardial I/R injury (Figure 1F)."
39897566
MYSM1 affects RUNX1
| 1
MYSM1 activates RUNX1. 1 / 1
| 1
reach
"Loss of MYSM1 was associated with increased H2AK119Ub levels, increased recruitment of PRC1 components Ring1B and Bmi1, and reduced binding of hematopoietic transcription factors Gata2 and Runx1 to th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24647359
MYSM1 affects RPL29
| 1
MYSM1 activates RPL29. 1 / 1
| 1
reach
"With the extension of the chronic restraint time, the expression of Mysm1 gradually increased in the MHb, HIP, and IC (Figure 1E)."
36414403
MYSM1 affects RPL10
| 1
RPL10 binds MYSM1. 1 / 1
| 1
sparser
"Furthermore, upon relocalization, RPL10 interacts with the MYB-like protein L10-interacting myb domain-containing protein (LIMYB) to suppress the expression of translational machinery genes, which results in enhanced tolerance to begomovirus infection [ xref ]."
30403659
MYSM1 affects RNF31, TAB2, and TAB3
| 1
TAB3 binds TAB2, MYSM1, and RNF31. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 affects RFC4
1 |
MYSM1 binds RFC4. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects RAS
| 1
RAS binds MYSM1. 1 / 1
| 1
sparser
"We found that certain domains, including Myb-like DNA-binding, Ras, kinase, DnaJ, and domains commonly associated with metabolic enzymes, were significantly enriched (Additional file xref : Fig. S15C)."
37024975
MYSM1 affects RAB5B
1 |
MYSM1 binds RAB5B. 1 / 1
1 |
biogrid
No evidence text available
40437099
MYSM1 affects Phosphatase
| 1
sparser
"In Arabidopsis, for instance, InsP 8 binding to SPX receptors promotes association of the PP-InsP/SPX complex with MYB-like PHR transcription factors ( xref ; xref ; xref ), known master regulators of phosphate starvation responses (PSRs) ( xref )."
38455731
MYSM1 affects Peritonitis
| 1
MYSM1 activates Peritonitis. 1 / 1
| 1
reach
"MYSM1 deficient mice were more susceptible to NOD2-mediated peritonitis, systemic inflammation and liver injury than wild-type mice."
33190004
MYSM1 affects PTGS2
| 1
MYSM1 decreases the amount of PTGS2. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects PRMT5
1 |
MYSM1 binds PRMT5. 1 / 1
1 |
biogrid
No evidence text available
17707232
MYSM1 affects PRDM1
| 1
MYSM1 activates PRDM1. 1 / 1
| 1
reach
"Furthermore, Mysm1 -/- plasma cells showed enhanced levels of antibody secretion in vitro and had altered gene expression profiles with downregulation of B cell lineage transcription factors Pax5 and Bach2, and increased expression of plasma cell transcription factors Blimp1 and Xbp1 [XREF_BIBR]."
32344625
MYSM1 affects PRC1_complex
| 1
MYSM1 inhibits PRC1_complex. 1 / 1
| 1
reach
"The authors concluded that MYSM1 antagonizes the action of the polycomb repressive complex 1 (PRC1) on the Ebf1 promoter, enabling lineage specific transcription factors, such as E2A, to be recruited to the Ebf1 locus and to induce its transcription [XREF_BIBR]."
22997486
MYSM1 affects PPP2
| 1
MYSM1 deubiquitinates PPP2. 1 / 1
| 1
reach
"MYSM1 enhanced the interaction between PP2A and RIPK2, further deubiquitinating and amplifying PP2A’s role in dephosphorylating RIPK2 at S176."
39746943
MYSM1 affects PPIB
1 |
MYSM1 binds PPIB. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects PPARGC1B
| 1
PPARGC1B binds MYSM1. 1 / 1
| 1
sparser
"Proteins were transferred onto PVDF (polyvinylidene difluoride) membranes in cold buffer (25 m m Tris Base, 192 m m glycine) by electrotransfer for 1.5 h, which were incubated in TBS buffer containing 5% milk for 1 h at 22–24 °C. The membrane was probed at 4 °C overnight with the following primary antibodies: Rabbit anti‐Mysm1 (1:1000, Abcam), rabbit anti‐PGC1 α (1:1000, Cell Signaling Technology), rabbit anti‐p‐p53 (1:1000, Cell Signaling Technology), rabbit anti‐p53 (1:1000, Cell Signaling Technology), rabbit anti‐p‐AMPK (1:1000, Cell Signaling Technology), rabbit anti‐AMPK (1:1000, Cell Signaling Technology), rabbit anti‐Sirt1 (1:1000, Cell Signaling Technology), rabbit anti‐p‐mTOR (1:1000, Cell Signaling Technology), rabbit anti‐mTOR(1:1000, Cell Signaling Technology), rabbit anti‐p‐S6 (1:1000, Cell Signaling Technology), mouse anti‐S6 (1:1000, Cell Signaling Technology), rabbit anti‐GAPDH (1:1000, Abclonal), mouse anti‐ β ‐actin (1:1000, APPLYGEN or Cell Signaling Technology)."
36414403
MYSM1 affects POLD1
| 1
POLD1 binds MSH6 and MYSM1. 1 / 1
| 1
sparser
"Furthermore, mild interactions of MYSM1 with POLD1 and MSH6 and with Lamin B1, involved in higher-order chromatin structure and anchoring of chromatin, were detectable."
32466590
MYSM1 affects PHLPP1
| 1
PHLPP1 binds MYSM1. 1 / 1
| 1
sparser
"The qRT-PCR analyses showed that siRNA-mediated downregulation of MYSM1 resulted in a significant decrease in PHLPP1 expression, confirming the association of MYSM1 with PHLPP1 ( xref )."
31761786
MYSM1 affects PAC
| 1
MYSM1 binds HTH and PAC. 1 / 1
| 1
sparser
"An unexpectedly potent size regulator that emerged from our screens was Dot6, a Myb-like HTH transcription factor that binds to the PAC (Polymerase A and C) motif [ xref ]."
30921326
MYSM1 affects P6.9
| 1
P6.9 binds MYSM1. 1 / 1
| 1
sparser
"Telomeric DNA is assembled into a non nucleosomal structure, whose main component is Rap1p, a duplex, sequence specific DNA binding protein that binds telomeric DNA with its Myb-like binding domain an[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19007917
MYSM1 affects OTULIN
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
MYSM1 affects OMIM #618116
| 1
MYSM1 activates OMIM #618116. 1 / 1
| 1
reach
"Recently, biallelic variants in MYSM1 have been reported to lead to a rare bone marrow failure (BMF) syndrome (OMIM #618116) in humans, which has been observed in only nine patients ( Al-Herz et al., [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
32640305
MYSM1 affects O41964
1 |
MYSM1 binds O41964. 1 / 1
1 |
virhostnet
No evidence text available
22028648
MYSM1 inhibits Neoplasm Metastasis. 1 / 1
| 1
reach
"Therefore, we hypothesized that MYSM-1 may suppress the metastasis of renal cell carcinoma in light of paucity of data regarding MYSM-1 in the cancers."
26409454
MYSM1 affects NUP43
1 |
NUP43 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37689310
MYSM1 affects NOS2
| 1
MYSM1 decreases the amount of NOS2. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects NOD2, RIPK2, and XIAP
| 1
MYSM1 binds NOD2, RIPK2, and XIAP. 1 / 1
| 1
sparser
"Although MYSM1 interacted with NOD2, RIP2, and XIAP, consistent with the above data (Fig.  xref ), we observed no direct interaction between MYSM1 and HOIP, TAK1, TAB2, TAB3, or the IKK complex (Fig. xref )."
30405132
MYSM1 affects NK lineage specification
| 1
MYSM1 inhibits NK lineage specification. 1 / 1
| 1
eidos
"In support of the role of ID2 in NK cell maturation , deficiency of MYSM1 , which mediates the recruitment of E4BP4 to the Id2 locus , leads to defects in NK cell maturation , but not NK lineage specification or commitment ( 23 ) ."
32849653
MYSM1 affects NF-κB-Luc
| 1
MYSM1 inhibits NF-κB-Luc. 1 / 1
| 1
reach
"The cGAS-STING-induced ISRE-Luc and NF-κB-Luc activities ( Figure 3 A), as well as ISRE activity induced by cGAS-STING or STING ( Figure 3 B), were suppressed by MYSM1."
33086059
MYSM1 affects Mice Lifespan MYSM1
| 1
MYSM1 activates Mice Lifespan MYSM1. 1 / 1
| 1
eidos
"MYSM1 Deficiency Reduces Mice Lifespan MYSM1 is a key regulator of tissue differentiation and development ."
33240758
MYSM1 affects MiR-129-5p
| 1
MiR-129-5p binds MYSM1. 1 / 1
| 1
sparser
"We further utilized a luciferase reporter assay to validate the direct interaction between miR-129-5p and Mysm1."
40344568
MYSM1 affects Mcl-1 mRNA
| 1
MYSM1 decreases the amount of Mcl-1 mRNA. 1 / 1
| 1
reach
"Among other survival genes differentially expressed in Mysm1 -/- and wild-type hematopoiesis, Mcl-1 mRNA levels were significantly decreased by factor 3 in Mysm1 -/- BM (XREF_FIG), whereas decreases in Bcl-X L mRNA were detectable to the same extent in Mysm1 -/- thymi (XREF_FIG)."
25613381
MYSM1 affects MYB
| 1
MYSM1 binds MYB and HTH. 1 / 1
| 1
sparser
"Both genes contained two introns, encoded 257 amino acids with two-Aa difference, and carried the unabridged HTH-MYB, MYB-like DNA-binding, and SANT domains."
31035916
MYSM1 affects MT-ATP6
| 1
MYSM1 increases the amount of MT-ATP6. 1 / 1
| 1
reach
"A deficiency in the transcription factor MYSM1 in mice or humans impairs RP expression and causes TP53-dependent bone marrow failure (58, 59)."
36413407
MYSM1 affects MSH6
| 1
POLD1 binds MSH6 and MYSM1. 1 / 1
| 1
sparser
"Furthermore, mild interactions of MYSM1 with POLD1 and MSH6 and with Lamin B1, involved in higher-order chromatin structure and anchoring of chromatin, were detectable."
32466590
MYSM1 affects MPN domain.According
| 1
MYSM1 binds STAT1 and MPN domain.According. 1 / 1
| 1
reach
"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain.According to our ubiquitinomics, the K379 residue of STAT1, located in the DNA binding domain (DBD) of STAT1 (Figure 6K), was the most significantly reduced deubiquitination residue of STAT1 after MYSM1 overexpression (Figure 5E)."
39897566
MYSM1 affects MMP
| 1
MYSM1 inhibits MMP. 1 / 1
| 1
reach
"JC-1 fluorescent probes demonstrated that MYSM1 knockdown elevated MMP while MYSM1 overexpression reduced MMP (Fig. 7J)."
39695708
MYSM1 affects MHb
| 1
MYSM1 activates MHb. 1 / 1
| 1
reach
"With the extension of the chronic restraint time, the expression of Mysm1 gradually increased in the MHb, HIP, and IC (Figure 1E)."
36414403
MYSM1 affects MEX3A
1 |
MEX3A binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37433992
MYSM1 affects MED25
| 1
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
MYSM1 affects MCMBP
1 |
MYSM1 binds MCMBP. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects MCM7
| 1
MYSM1 decreases the amount of MCM7. 1 / 1
| 1
reach
"Western blot analysis showed that deletion of Mysm1 significantly increased the expression of Mcm2, phosphorylated p53 (p-p53), Puma, and Bax in NSCs (Fig. 2H)."
38342917
MYSM1 affects MAPK
| 1
MYSM1 inhibits MAPK. 1 / 1
| 1
reach
"Significant reduction of MYSM1 protein expression was detectable upon treatment of A375 cells with either inhibitor PD128,345, targeting mitogen activated protein kinase kinase MEK, alone or in combination with an Akt1/2-inhibitor for 24 hrs."
28978033
MYSM1 affects MACROH2A1
1 |
MYSM1 binds MACROH2A1. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects Lymphoma, B-Cell
| 1
reach
"Loss of MYSM1 inhibits the oncogenic activity of cMYC in B cell lymphoma."
34114734
MYSM1 affects Leu-Lys
| 1
MYSM1 inhibits Leu-Lys. 1 / 1
| 1
reach
"We found that MYSM1 deficiency significantly reduced the proportion of LSK cells and LK cells in the G 0 phase, whereas there was a significant increase in the proportion of LSK cells and LK cells in G 1 phase (XREF_FIG)."
29694335
MYSM1 affects LSK
| 1
MYSM1 inhibits LSK. 1 / 1
| 1
reach
"We found that MYSM1 deficiency significantly reduced the proportion of LSK cells and LK cells in the G 0 phase, whereas there was a significant increase in the proportion of LSK cells and LK cells in G 1 phase (XREF_FIG)."
29694335
MYSM1 affects KIF11
1 |
MYSM1 binds KIF11. 1 / 1
1 |
biogrid
No evidence text available
17707232
MYSM1 affects K48- linked polyubiquitins
| 1
MYSM1 binds K48- linked polyubiquitins. 1 / 1
| 1
reach
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
MYSM1 affects IgM
| 1
MYSM1 activates IgM. 1 / 1
| 1
reach
"This suggests that miR-150 is involved in IgM enhancement induced by MYSM1 deficiency."
27590507
MYSM1 affects IRF3
| 1
MYSM1 inhibits IRF3. 1 / 1
| 1
reach
"Interestingly, type I IFN, pro-inflammatory cytokines, TBK1, and IRF3 induced by DNA sensing were downregulated by MYSM1, upregulated by sh- Mysm1 , and enhanced in Mysm1 −/− mice."
33086059
MYSM1 affects IPO4
1 |
MYSM1 binds IPO4. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects IL1
| 1
MYSM1 decreases the amount of IL1. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects IKK_complex
| 1
MYSM1 increases the amount of IKK_complex. 1 / 1
| 1
reach
"To confirm MYSM1’s specific role in these signaling pathways, Mysm1 plasmids were reintroduced into Mysm1-KO chondrocytes, significantly restoring the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 (Fig. S4D-E)."
39746943
MYSM1 affects IFNB1
| 1
MYSM1 inhibits IFNB1. 1 / 1
| 1
reach
"Interestingly, a recovery experiment demonstrated that IFN-β , IL-6, and Cxcl10 mRNA induced by HSV-1 in MEFs derived from Mysm1 −/− mice were significantly suppressed by the overexpression of MYSM1 ([MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MYSM1 affects IFN-I
| 1
MYSM1 activates IFN-I. 1 / 1
| 1
reach
"Notably, UCHL1, OTUB1, OTUB2, OTUD5, USP25, MYSM1, and MCPIP1 (Figure 3A) negatively regulate virus-induced IFN-I production and antiviral activities by cleaving K63-linked or other types of polyubiquitin chains from TRAF3 or TRAF6."
34707613
MYSM1 affects Hypertrophy
| 1
MYSM1 activates Hypertrophy. 1 / 1
| 1
reach
"In addition, MYSM1 mediates cardiac hypertrophy through PARP1-dependent cardiomyocyte parthanatos."
39907013
MYSM1 affects Histone_H2B
| 1
reach
"It would be intriguing to examine whether MYSM1 could associate with ubiquitinated chromatin proteins, such as histones H2A and H2B."
17428495
MYSM1 affects HSPA1A
1 |
MYSM1 binds HSPA1A. 1 / 1
1 |
biogrid
No evidence text available
17707232
MYSM1 affects HSP90AB2P
1 |
MYSM1 binds HSP90AB2P. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects HNRNPH1
1 |
MYSM1 binds HNRNPH1. 1 / 1
1 |
biogrid
No evidence text available
17707232
MYSM1 affects HATs
| 1
MYSM1 binds HATs. 1 / 1
| 1
reach
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
MYSM1 affects H3K4Me3
| 1
MYSM1 increases the amount of H3K4Me3. 1 / 1
| 1
reach
"Moreover, the results showed that MYSM1 depletion inhibited H3K4me3, H3K9ac and H3K27ac levels on ERE regions, suggesting that MYSM1 may crosstalk with the HATs complex to modulate histone modifications on ERE regions of ERα-regulatory genes in BCa-derived cells (Fig. 4D; Appendix Fig. S2A–C)."
38177530
MYSM1 affects H3K36M3
| 1
MYSM1 inhibits H3K36M3. 1 / 1
| 1
reach
"More importantly, both pS2-Pol II and H3K36M3 were remarkably diminished by siRNA of 2A-DUB even in the presence of DHT, mainly on the exon 4 region."
17707232
MYSM1 affects H3-3B
1 |
H3-3B binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
36089195
MYSM1 affects H2BC10
1 |
H2BC10 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
MYSM1 affects H2AK119
| 1
MYSM1 deubiquitinates H2AK119. 1 / 1
| 1
reach
"To confirm that the observed up-regulation of lamin A/C was due to specific knockdown of Ring1 or Rnf2 rather than off-target effects, we overexpressed Mysm1, which deubiquitinates H2AK119 in opposition to Ring1 and Rnf2."
30072429
MYSM1 affects GREB1
| 1
MYSM1 increases the amount of GREB1. 1 / 1
| 1
reach
"MYSM1 depletion significantly reduced the eRNA expression of c-Myc, CCND1, E2F1, GREB1, and TFF1, indicating that MYSM1-recruited enhancers exhibited increased transcriptional activity (Fig. EV3C)."
38177530
MYSM1 affects GATA2
| 1
MYSM1 activates GATA2. 1 / 1
| 1
reach
"Loss of MYSM1 was associated with increased H2AK119Ub levels, increased recruitment of PRC1 components Ring1B and Bmi1, and reduced binding of hematopoietic transcription factors Gata2 and Runx1 to th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24647359
MYSM1 affects Figure 7J-K
| 1
MYSM1 activates Figure 7J-K. 1 / 1
| 1
reach
"Notably, STAT1 knockdown (STAT1-KD, Figure S6J) reversed MYSM1-mediated up-regulation of Mlkl and Ripk3 mRNAs (Figure 7J-K), confirming that MYSM1 transcriptionally increased the expression of necroptosis-related genes via STAT1."
39897566
MYSM1 affects Fig.
| 1
MYSM1 inhibits Fig.. 1 / 1
| 1
reach
"However, MYSM1 overexpression reduced both total and RIPK2 ubiquitination and p-RIPK2 S176 levels induced by IL-1β (Fig. 5o–t)."
39746943
MYSM1 affects Ebf1beta promoter
| 1
Modified MYSM1 activates Ebf1beta promoter. 1 / 1
| 1
reach
"MYSM1 expression activated transcription of the Ebf1alpha promoter and modestly activated the Ebf1beta promoter, but only marginally activated the Pax5 promoter and Cd79a promoter."
22169041
MYSM1 affects Ebf1alpha promoter
| 1
Modified MYSM1 increases the amount of Ebf1alpha promoter. 1 / 1
| 1
reach
"MYSM1 expression activated transcription of the Ebf1alpha promoter and modestly activated the Ebf1beta promoter, but only marginally activated the Pax5 promoter and Cd79a promoter."
22169041
MYSM1 affects ERK
| 1
MYSM1 increases the amount of ERK. 1 / 1
| 1
reach
"To confirm MYSM1’s specific role in these signaling pathways, Mysm1 plasmids were reintroduced into Mysm1-KO chondrocytes, significantly restoring the phosphorylation levels of IKKα/β, P65, IKBα, JNK, ERK, and P38 (Fig. S4D-E)."
39746943
MYSM1 affects EAF1
| 1
EAF1 binds MYSM1. 1 / 1
| 1
sparser
"The answer is that the C-terminal MyB-like domain of Eaf1 binds to the surface occupied by SAGA-specific subunits (), ensuring the formation of two distinct complexes."
36417436
MYSM1 affects Disease
| 1
MYSM1 activates Disease. 1 / 1
| 1
reach
"Aberrant expression of Mysm1 leads to different diseases, including bone marrow failure disorder, autoimmune diseases, cancer, and depression [12–18]."
38342917
MYSM1 affects DPEP1
| 1
MYSM1 inhibits DPEP1. 1 / 1
| 1
reach
"118 Expression of MYSM1 decreases MDP stimulated NOD2 activation in cells, and in mouse models, knockout of MYSM1 leads to increased inflammation and liver damage, showing the DUB to be a critical negative regulator of NOD2 signaling."
30890292
MYSM1 affects DOX
| 1
MYSM1 activates DOX. 1 / 1
| 1
reach
"Loss- and gain- of function analysis further confirmed that MYSM1 promotes DOX-induced cardiomyocyte injury in vitro."
39695708
MYSM1 affects DNA repair
| 1
MYSM1 activates DNA repair. 1 / 1
| 1
eidos
"Collectively , these results revealed that MYSM1 deficiency represses DNA repair , whereas MYSM1 over-expression promotes DNA repair ."
33240758
MYSM1 affects DNA damage-induced senescence
| 1
MYSM1 inhibits DNA damage-induced senescence. 1 / 1
| 1
eidos
"Higher numbers of TUNEL-positive cells were observed in the liver of AAV9-Ctrl-treated mice compared to AAV9-Mysm1-treated mice ( Figure S11A , Supporting Information ) , confirming that MYSM1 suppresses DNA damage-induced senescence and apoptosis ."
33240758
MYSM1 activates DNA Breaks, Double-Stranded. 1 / 1
| 1
reach
"MYSM1 deficiency spontaneously perturbs the proper repair of DNA damage to accumulate DNA double strand breaks (DSB), accompanied by cellular senescence (Kroeger et al, 2020; Nishi et al, 2014; Tian et al, 2020a)."
38177530
MYSM1 affects DICER1
1 |
DICER1 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
24778252
MYSM1 affects DHX40
1 |
DHX40 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
MYSM1 affects DHPS
| 1
MYSM1 binds DHPS. 1 / 1
| 1
sparser
"At least one shelterin component, Rap1, is present in S. cerevisiae , although unlike vertebrate RAP1, ScRap1p directly binds ds telomeric DNA through two myb-like DNA binding domains and contributes to telomere length regulation and telomere silencing ( xref )."
19854131
MYSM1 affects DDR-Associated SASP
| 1
MYSM1 inhibits DDR-Associated SASP. 1 / 1
| 1
eidos
"MYSM1 Suppresses DDR-Associated SASP As DNA damage accumulation is an important factor that induces SASP , [ ."
33240758
MYSM1 affects Cyclin
| 1
MYSM1 binds Cyclin. 1 / 1
| 1
sparser
"Hirai, H.; Sherr, C.J. Interaction of D-type Cyclins with a Novel Myb-like Transcription Factor, DMP1."
11853005
MYSM1 affects Cellularity
| 1
MYSM1 activates Cellularity. 1 / 1
| 1
reach
"Loss of Mysm1 Causes Skin Atrophy and Reduced Skin Cellularity in Mysm1 Deficient Mice."
29495602
MYSM1 affects Cellular Senescence Aging Process Lifespan Abstract Aging
| 1
MYSM1 inhibits Cellular Senescence Aging Process Lifespan Abstract Aging. 1 / 1
| 1
eidos
"MYSM1 Suppresses Cellular Senescence and the Aging Process to Prolong Lifespan Abstract Aging is a universal feature of life that is a major focus of scientific research and a risk factor in many diseases ."
33240758
MYSM1 affects Carcinogenesis
| 1
reach
"MYSM1 inhibits human colorectal cancer tumorigenesis by activating miR-200 family members/CDH1 and blocking PI3K/AKT signaling."
34706761
MYSM1 affects CYLD
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
MYSM1 affects CHEK2
| 1
MYSM1 inhibits CHEK2. 1 / 1
| 1
reach
"Higher expression of MYSM1 also consistently reduced p-CHK2 (Fig 5, A and B); although, this did not reach statistical significance because the magnitude of reduction was more variable (Fig 5, B)."
38065233
MYSM1 affects CENPA
1 |
CENPA binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
MYSM1 affects CDKN2A
| 1
MYSM1 inhibits CDKN2A. 1 / 1
| 1
reach
"Lymphoid cell depletion in Mysm1 deficiency was previously attributed to several mechanisms, involving either MYSM1 mediated Cdkn2a and p19 ARF repression to restrict p53 activation in thymocytes, 39 or MYSM1 mediated induction of lymphoid-lineage genes, such as Ebf1 and Id2."
26768662
MYSM1 affects CDKN1A
| 1
MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
| 1
sparser
"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
26768662
MYSM1 affects CDK8
1 |
CDK8 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
29568061
MYSM1 affects CD8
| 1
MYSM1 activates CD8. 1 / 1
| 1
reach
"Mysm1 ablation resulted in a twofold reduction in CD8 + T-cell numbers, and also led to a hyperactivated CD8 + T-cell state accompanied by impaired proliferation and increased pro inflammatory cytokine production after ex vivo stimulation."
28066899
MYSM1 affects CD79A
| 1
Modified MYSM1 activates CD79A. 1 / 1
| 1
reach
"MYSM1 expression activated transcription of the Ebf1alpha promoter and modestly activated the Ebf1beta promoter, but only marginally activated the Pax5 promoter and Cd79a promoter."
22169041
MYSM1 affects CD34
| 1
MYSM1 activates CD34. 1 / 1
| 1
reach
"Though iron transport, storage, and metabolism as well as antioxidant genes were transcriptionally upregulated or remained unchanged, the levels of key proteins including GPX4, SLC7A11, and FTH1 were all significantly reduced in HSPCs and most profoundly reduced in the primitive HSC-enriched CD34 CD45RA CD90 compartment, which could be rescued by re-expressing WT MYSM1, but not the disease-associated loss-of-function mutants (Figure 6A–B, S6A–B)."
36803603
MYSM1 affects CACNB2
| 1
MYSM1 binds CACNB2. 1 / 1
| 1
sparser
"Briefly, CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) are morning-expressed MYB-like transcription factors that bind directly to the promoter of TIMING OF CAB2 EXPRESSION1 ( T[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22784827
MYSM1 affects BRCC36
| 1
MYSM1 binds BRCC36. 1 / 1
| 1
sparser
"Importantly, MYSM1 and BRCC36 are associated with complexes that activate their deubiquitinating activity."
26918171
MYSM1 affects BRAT1
1 |
MYSM1 binds BRAT1. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYSM1 affects BMI1
| 1
MYSM1 inhibits BMI1. 1 / 1
| 1
reach
"Loss of MYSM1 was associated with increased H2AK119Ub levels, increased recruitment of PRC1 components Ring1B and Bmi1, and reduced binding of hematopoietic transcription factors Gata2 and Runx1 to th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24647359
MYSM1 affects B1a
| 1
MYSM1 activates B1a. 1 / 1
| 1
reach
"To identify potential miRNAs that are involved in MYSM1 induced B1a abnormality, we performed Quantitative Reverse Transcription PCR (qRT-PCR) assays to analyze the expression level of these miRNAs in B1a cells."
27590507
MYSM1 affects Autoimmunity
| 1
MYSM1 inhibits Autoimmunity. 1 / 1
| 1
eidos
"MYSM1 Represses Innate Immunity and Autoimmunity through Suppressing the cGAS-STING Pathway ."
33086059
MYSM1 affects Atrophy
| 1
MYSM1 inhibits Atrophy. 1 / 1
| 1
reach
"Loss of Mysm1 Causes Skin Atrophy and Reduced Skin Cellularity in Mysm1 Deficient Mice."
29495602
MYSM1 affects Akt/c-Raf/GSK-3beta
| 1
MYSM1 inhibits Akt/c-Raf/GSK-3beta. 1 / 1
| 1
reach
"MYSM1 was able to bind to androgen receptor instead of increasing its expression and knockdown of MYSM1 resulted in activation of Akt/c-Raf/GSK-3beta signaling."
31761786
MYSM1 affects AURKA
1 |
MYSM1 binds AURKA. 1 / 1
1 |
biogrid
No evidence text available
33669244
MYSM1 affects ATG14
1 |
ATG14 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37632749
MYSM1 affects ASGR1
| 1
MYSM1 activates ASGR1. 1 / 1
| 1
reach
"Next, we verified the elevated mRNA levels of MYSM1 in H/R-induced HL-1 cells (Figure 1E) and myocardial I/R injury (Figure 1F)."
39897566
MYSM1 affects AKT/c-Raf/GSK-3β
| 1
MYSM1 inhibits AKT/c-Raf/GSK-3β. 1 / 1
| 1
reach
"In addition, MYSM1 interacts with the AR and reduces AR activity by inhibiting AKT/c-Raf/GSK-3β signaling (78)."
37732538
MYSM1 affects AKT
| 1
MYSM1 inhibits AKT. 1 / 1
| 1
reach
"MYSM1 inhibited the CRC progression via miR-200/CDH1 and inhibition of PI3K/AKT axis [35]."
37580737
MYSM1 affects AF_1519
| 1
MYSM1 activates AF_1519. 1 / 1
| 1
reach
"The results showed that MYSM1 simultaneously increased ERα-AF1 and AF2 actions (Figs."
38177530
MYSM1 affects ADAMTS5
| 1
MYSM1 decreases the amount of ADAMTS5. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects ADAMTS3
| 1
MYSM1 decreases the amount of ADAMTS3. 1 / 1
| 1
reach
"Similarly, silencing Mysm1 with si-Mysm1 enhanced IL-1β-induced levels of MMP3, MMP13, ADAMTS4, ADAMTS5, COX2, and iNOS (Fig. S2A–F)."
39746943
MYSM1 affects ACTBL2
1 |
MYSM1 binds ACTBL2. 1 / 1
1 |
biogrid
No evidence text available
32466590
MYB affects MYSM1
| 1
MYSM1 binds MYB and HTH. 1 / 1
| 1
sparser
"Both genes contained two introns, encoded 257 amino acids with two-Aa difference, and carried the unabridged HTH-MYB, MYB-like DNA-binding, and SANT domains."
31035916
MSH6 affects POLD1
| 1
POLD1 binds MSH6 and MYSM1. 1 / 1
| 1
sparser
"Furthermore, mild interactions of MYSM1 with POLD1 and MSH6 and with Lamin B1, involved in higher-order chromatin structure and anchoring of chromatin, were detectable."
32466590
MPN domain.According affects STAT1
| 1
MYSM1 binds STAT1 and MPN domain.According. 1 / 1
| 1
reach
"These data indicated that MYSM1 interacts with STAT1 through the SWIRM domain and deubiquitinates STAT1 through the MPN domain.According to our ubiquitinomics, the K379 residue of STAT1, located in the DNA binding domain (DBD) of STAT1 (Figure 6K), was the most significantly reduced deubiquitination residue of STAT1 after MYSM1 overexpression (Figure 5E)."
39897566
MPN domain affects STING1
| 1
MYSM1 binds STING1 and MPN domain. 1 / 1
| 1
reach
"We have shown that the SWIRM domain is necessary for MYSM1 interaction with STING, and the MPN domain is necessary for STING deubiquitination.In addition to the defense against viral infections, STING[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
MIR484 affects MYSM1
1 |
MIR484 decreases the amount of MYSM1. 1 / 1
1 |
biopax:mirtarbase
No evidence text available
23622248
MEX3A affects MYSM1
1 |
MEX3A binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37433992
MED25 affects TCEA1
| 1
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
MCMBP affects MYSM1
1 |
MYSM1 binds MCMBP. 1 / 1
1 |
biogrid
No evidence text available
32466590
MACROH2A1 affects MYSM1
1 |
MYSM1 binds MACROH2A1. 1 / 1
1 |
biogrid
No evidence text available
32466590
KIF11 affects MYSM1
1 |
MYSM1 binds KIF11. 1 / 1
1 |
biogrid
No evidence text available
17707232
K48- linked polyubiquitins affects MYSM1
| 1
MYSM1 binds K48- linked polyubiquitins. 1 / 1
| 1
reach
"Cytoplasmic MYSM1 associated with K63- and K48- linked polyubiquitins, TRAF3 and TRAF6, but not pCAF ( Figure 4 G)."
26474655
IRF8 affects MYSM1
| 1
IRF2 binds IRF8 and MYSM1. 1 / 1
| 1
sparser
"We then tested Mysm1 binding to the promoter regions of IRF2 and IRF8 by analyzing WT (not Mysm1 −/− ) Lin − BM cells via a ChIP assay after in vitro stimulation with Poly(I:C) or IFN-γ."
27277682
IPO4 affects MYSM1
1 |
MYSM1 binds IPO4. 1 / 1
1 |
biogrid
No evidence text available
32466590
IL6 affects MYSM1
| 1
IL6 activates MYSM1. 1 / 1
| 1
reach
"The levels of IFN-β , ISG56 , ISG15 , TNF-α , IL-6 , and CXCL10 mRNAs were markedly elevated in Lenti- Mysm1 -treated PBMCs isolated from SLE patients ( Figures 7 K–7P), documenting that MYSM1 repress[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
IFNG affects MYSM1
| 1
IFNG activates MYSM1. 1 / 1
| 1
eidos
"Mysm1 , a histone deubiquitinase , is induced by TNF-alpha and IFNgamma in ASCs and promotes miR-150 transcription , which enhances iNOS production ."
32363193
Histone_H2B affects MYSM1
| 1
reach
"It would be intriguing to examine whether MYSM1 could associate with ubiquitinated chromatin proteins, such as histones H2A and H2B."
17428495
His2A affects MYSM1
| 1
His2A activates MYSM1. 1 / 1
| 1
reach
"Significantly, although cells were treated with DHT as usual, the amount of pS5-Pol II on the promoter was reduced upon loss of 2A-DUB and increased levels of uH2A, which argues that 2A-DUB and result[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17707232
HTH affects PAC
| 1
MYSM1 binds HTH and PAC. 1 / 1
| 1
sparser
"An unexpectedly potent size regulator that emerged from our screens was Dot6, a Myb-like HTH transcription factor that binds to the PAC (Polymerase A and C) motif [ xref ]."
30921326
HTH affects MYB, and MYSM1
| 1
MYSM1 binds MYB and HTH. 1 / 1
| 1
sparser
"Both genes contained two introns, encoded 257 amino acids with two-Aa difference, and carried the unabridged HTH-MYB, MYB-like DNA-binding, and SANT domains."
31035916
HSPA1A affects MYSM1
1 |
MYSM1 binds HSPA1A. 1 / 1
1 |
biogrid
No evidence text available
17707232
HSP90AB2P affects MYSM1
1 |
MYSM1 binds HSP90AB2P. 1 / 1
1 |
biogrid
No evidence text available
32466590
HNRNPH1 affects MYSM1
1 |
MYSM1 binds HNRNPH1. 1 / 1
1 |
biogrid
No evidence text available
17707232
HELLS affects RFC5
| 1
RFC5 binds HELLS, PCNA, and MYSM1. 1 / 1
| 1
sparser
"In summary, our current results support a role of MYSM1 in DDR: (1) MYSM1 was recruited to DNA lesions induced by etoposide in vitro in human PBMC, KG-1a myeloid leukemia cells, and in melanoma cells. (2) In mass spectrometry analyses, known DNA repair and replication factors, including helicase HELLS and RFC4/5, were identified as novel candidate interaction partners of MYSM1 upon DNA damage induction in 293T cells. (3) The specificity of the interactions of MYSM1 with RFC5, HELLS, and PCNA could be verified by direct and reverse co-IP, and (4) correlated with accumulation of γH2AX in HSPC from aged Mysm1-deficient mice."
32466590
HATs affects MYSM1
| 1
MYSM1 binds HATs. 1 / 1
| 1
reach
"The finding that two DUBs, 2A-DUB and USP22 associate with HATs suggests an important functional interplay between these classes of enzymes."
18430235
H3-3B affects MYSM1
1 |
H3-3B binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
36089195
H2BC10 affects MYSM1
1 |
H2BC10 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
Gentamicins affects MYSM1
1 |
Gentamicins increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
33387578
GFI1 affects MYSM1
| 1
MYSM1 binds GFI1. 1 / 1
| 1
sparser
"We found that Mysm1 directly associates with the Gfi1 enhancer element and promotes its transcription through Gata2 and Runx1 transactivation."
24014243
FLT3 affects MYSM1
| 1
MYSM1 binds FLT3. 1 / 1
| 1
sparser
"MYSM1 bound to the Flt3 gene promoter, and loss of MYSM1 resulted in reduced recruitment of the major hematopoietic transcription factor PU.1 and an increase in repressive histone marks H2AK119ub and H3K27me3 at the Flt3 locus [ xref ] ( xref )."
32344625
EAF1 affects MYSM1
| 1
EAF1 binds MYSM1. 1 / 1
| 1
sparser
"The answer is that the C-terminal MyB-like domain of Eaf1 binds to the surface occupied by SAGA-specific subunits (), ensuring the formation of two distinct complexes."
36417436
E3_Ub_ligase affects MYSM1
| 1
E3_Ub_ligase activates MYSM1. 1 / 1
| 1
sparser
"This complex results in degradation of the DELLA protein through the E3 ubiquitin ligase, which can activate a downstream myb-like transcription factor (GAMyb)."
37993774
Dronabinol affects MYSM1
1 |
Dronabinol increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
29691375
DREB2A affects TCEA1
| 1
MED25 binds TCEA1, DREB2A, and MYSM1. 1 / 1
| 1
sparser
"MED25 physically interacts with abiotic-stress associated TFs, DREB2A, ZFHD1, and MYB-Like ( xref ;Table xref )."
28659948
DOX affects MYSM1
| 1
DOX increases the amount of MYSM1. 1 / 1
| 1
reach
"RT-qPCR further showed that DOX significantly increased the mRNA levels of Mysm1 in cardiomyocytes rather than non-cardiomyocytes (Fig. 1J)."
39695708
DNA90 affects MYSM1
| 1
DNA90 activates MYSM1. 1 / 1
| 1
reach
"Similarly, in mouse bone marrow-derived macrophages (BMDMs), Mysm1 mRNA was activated by HSV-1 ( Figure 1 E), poly(dA:dT), ISD 45, DNA90, and HSV120 ( Figure 1 F), as well as by cGAMP ( Figure 1 G)."
33086059
DICER1 affects MYSM1
1 |
DICER1 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
24778252
DHX40 affects MYSM1
1 |
DHX40 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
DHPS affects MYSM1
| 1
MYSM1 binds DHPS. 1 / 1
| 1
sparser
"At least one shelterin component, Rap1, is present in S. cerevisiae , although unlike vertebrate RAP1, ScRap1p directly binds ds telomeric DNA through two myb-like DNA binding domains and contributes to telomere length regulation and telomere silencing ( xref )."
19854131
DEGs affects MYSM1
| 1
DEGs inhibits MYSM1. 1 / 1
| 1
reach
"DEGs involved in epigenetic regulation include upregulatd NSD1 and downregulated KDM5A and MYSM1 (XREF_SUPPLEMENTARY)."
26324762
Cyclin affects MYSM1
| 1
MYSM1 binds Cyclin. 1 / 1
| 1
sparser
"Hirai, H.; Sherr, C.J. Interaction of D-type Cyclins with a Novel Myb-like Transcription Factor, DMP1."
11853005
CYLD affects TNFAIP3
| 1
RNF31 binds CYLD, OTULIN, TNFAIP3, and MYSM1. 1 / 1
| 1
sparser
"Upon immunoprecipitation, we observed no direct interaction between MYSM1 with HOIP, NEMO or the negative regulators CYLD, OTULIN or A20 (Fig.  xref )."
30405132
CXCL10 affects MYSM1
| 1
CXCL10 activates MYSM1. 1 / 1
| 1
reach
"The levels of IFN-β , ISG56 , ISG15 , TNF-α , IL-6 , and CXCL10 mRNAs were markedly elevated in Lenti- Mysm1 -treated PBMCs isolated from SLE patients ( Figures 7 K–7P), documenting that MYSM1 repress[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
33086059
CENPA affects MYSM1
1 |
CENPA binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
34079125
CDKN1A affects TP53
| 1
MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
| 1
sparser
"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
26768662
CDK8 affects MYSM1
1 |
CDK8 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
29568061
CACNB2 affects MYSM1
| 1
MYSM1 binds CACNB2. 1 / 1
| 1
sparser
"Briefly, CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) are morning-expressed MYB-like transcription factors that bind directly to the promoter of TIMING OF CAB2 EXPRESSION1 ( T[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22784827
BRCC36 affects MYSM1
| 1
MYSM1 binds BRCC36. 1 / 1
| 1
sparser
"Importantly, MYSM1 and BRCC36 are associated with complexes that activate their deubiquitinating activity."
26918171
BRAT1 affects MYSM1
1 |
MYSM1 binds BRAT1. 1 / 1
1 |
biogrid
No evidence text available
32466590
BMF affects MYSM1
| 1
BMF activates MYSM1. 1 / 1
| 1
reach
"Variations in MYSM1 cause BMF that follows an autosomal recessive inherited model ( https://omim.org/entry/612176 ); thus, we next examined whether MYSM1 harbored another heterozygous variant."
32640305
BBC3 affects TP53
| 1
MYSM1 binds BBC3, CDKN1A, and TP53. 1 / 1
| 1
sparser
"Mechanistically, we find that the MYSM1 protein associates with p53 and colocalizes to promoters of classical p53-target genes Bbc3 /PUMA (p53 upregulated modulator of apoptosis) and Cdkn1a /p21."
26768662
BACH2 affects MYSM1
| 1
BACH2 activates MYSM1. 1 / 1
| 1
reach
"XREF_FIG shows that mRNA levels of Pax5 and Bach2 were significantly reduced, while Blimp1 and Xbp1 mRNA levels were significantly elevated in naive and activated Mysm1 -/- B cells."
26348977
Adenoviridae Infections increases the amount of MYSM1. 1 / 1
| 1
reach
"For example, adenovirus infection leads to increased transcription of MYSM1, a repressor of STING signaling, thus enabling the virus to reduce IFN-I expression activated through the STING/TBK1/IRF3 pathway [67]."
35632630
AURKA affects MYSM1
1 |
MYSM1 binds AURKA. 1 / 1
1 |
biogrid
No evidence text available
33669244
ATG14 affects MYSM1
1 |
ATG14 binds MYSM1. 1 / 1
1 |
biogrid
No evidence text available
37632749
ARF affects TP53
| 1
TP53 binds ARF and MYSM1. 1 / 1
| 1
sparser
"In line with a previous report on Mysm1-binding to thep19 ARF -promoter in thymocytes [ xref ], an upstream regulator of TS p53, in Mysm1 −/− epidermal cells as well, mRNA expression ofp19 ARF was moderately increased along with increases in p53 levels and in apoptosis."
29495602
ACTBL2 affects MYSM1
1 |
MYSM1 binds ACTBL2. 1 / 1
1 |
biogrid
No evidence text available
32466590
AAV9 affects MYSM1
| 1
AAV9 inhibits MYSM1. 1 / 1
| 1
reach
"Correspondingly, cardiomyocyte-specific knockdown of MYSM1 by AAV9 protected the heart from DOX-induced cardiotoxicity."
39695708
6-propyl-2-thiouracil decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
30047161
3,4-methylenedioxymethamphetamine increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
20188158
2,3,7,8-tetrachlorodibenzodioxine decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
19770486
17alpha-ethynylestradiol increases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
23129252
1,2-dimethylhydrazine decreases the amount of MYSM1. 1 / 1
1 |
ctd
No evidence text available
22206623