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"We recently identified mediator in Arabidopsis thaliana and we have also reported that the Med25 subunit in Arabidopsis interacts with three different transcription factors, Dreb2a, Zfhd1 and myb-like which all are involved in different stress response pathways ( xref , xref ) In this article, we use a set of biochemical and biophysical methods to study interaction between the Med25 mediator subunit and the transcription factor Dreb2a from A. thaliana ."