IndraLab

Statements

ZDHHC2 binds MYSM1. 5 / 5
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"In further support of these findings, incubation of Ub-RIP2 immunoprecipitated from Mysm1 −/− BMDMs with Rec-MYSM1 resulted in diminished K63-, K27-, and M1-linked, but not K48-linked, ubiquitin chains (Supplementary Fig.  xref )."
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"In contrast, Rec-MYSM1 had no effect on ubiquitin chains attached to NEMO (Supplementary Fig.  xref ), indicating that MYSM1 was selectively cleaving K63, K27, and M1, but not K48 linkages, from RIP2."
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"Moreover, in vitro, Rec-MYSM1 could remove polyubiquitins from RIP2, but not NEMO (Supplementary Figure  xref )."
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"Next we purified ubiquitin conjugates from L18-MDP-stimulated Mysm1 − /− BMDMs by TUBE and incubated them with recombinant MYSM1 (Rec-MYSM1) and compared the ubiquitin restriction pattern of Ub-RIP2 with that of AMSH, UCHL3, OTULIN or a combination of these three DUBs (Fig.  xref )."
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"To gain a complete overview of the ubiquitin linkage specificity of MYSM1, we incubated Rec-MYSM1 with Di-ubiqutins (Ub 2 ) of different linkages."
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