IndraLab

Statements

Tamoxifen activates MYSM1. 5 / 5
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"14 The EuMyc mouse line was crossed to our established Mysm1 and Mysm1 Cre mouse lines, allowing either constitutive or tamoxifen‐induced Mysm1‐deletion."
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"The Mysm1 fl/fl line was generated as previously described [13] and crossed to the Gt(ROSA)26Sor tm1(cre/ERT2) line for tamoxifen-induced Mysm1 inactivation."
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"Mysm1 flox and flox line was generated by crossing Mysm1 tm1a mice to the Gt (ROSA) 26 Sor tm1 (FLP1) Dym and Wtsi line with ubiquitous expression of Flp recombinase, 56 and subsequently bred to Gt (ROSA) 26Sor tm1 (cre and ERT2) mice for tamoxifen induced Mysm1 inactivation."
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"For tamoxifen induced Mysm1 gene inactivation in the Mysm1 flox and flox Gt (ROSA) 26Sor tm1 (cre and ERT2) mouse line, the mice were injected intraperitoneally with tamoxifen (T5648, Sigma, St. Louis, MO, USA) in sterilized corn oil (Sigma C8267) at 0.15 mg/g per injection, with eight doses in total administered over 16 days."
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"For this purpose, wild-type bone marrow was transplanted into Mysm1 fl/fl Tg.CreERT2 recipients, followed by tamoxifen-induced ablation of Mysm1 specifically within non-hematopoietic cells of the reci[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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