IndraLab

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MYSM1 binds MYC. 7 / 7
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"Here, we show that MYSM1 and c-Myc bind to the same region (approximately 1200–1000 nt upstream) of the promoter of miR-150 in B1a cells from WT mice but c-Myc cannot bind to the promoter in MYSM1 −/− mice."
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"10 To further validate the overlap in the genomic binding sites of MYSM1 and cMYC in Ba/F3 cells, the binding of cMYC at the MYSM1‐binding sites of select genes encoding RPs and translation factors was tested and confirmed with ChIP‐qPCR (Figure 1E, Supporting Information S1)."
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"The binding of MYSM1 and cMYC at the shared sites was also confirmed in cells derived from EuMyc mouse B cell lymphoma, 14 , 15 specifically for the Rpl7 and Eef1g gene promoters (data not shown)."
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"Overall, we demonstrate shared DNA binding of cMYC and MYSM1 at the promoters of genes encoding ribosomal proteins and translation factors."
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"Meanwhile, co-Immunoprecipitation (co-IP) was performed to detect the interaction between MYSM1 and c-Myc and results showed that MYSM1 protein co-immunoprecipitated with c-Myc in murine RAW264.7 cells."
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"Meanwhile, co-Immunoprecipitation (co-IP) was performed to detect the interaction between MYSM1 and c-Myc and results showed that MYSM1 protein co-immunoprecipitated with c-Myc in murine RAW264.7 cells (Figure xref )."
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"Overall, this suggested a possible cooperation between cMYC and MYSM1 in the transcriptional regulation of genes encoding ribosomal proteins and translation factors.We have previously validated MYSM1 binding to the promoters of RP genes in Ba/F3 cells by ChIP‐qPCR, and also demonstrated a reduction in RP‐gene expression in Mysm1‐shRNA knockdown Ba/F3 cells and in Mysm1‐deficient primary haematopoietic stem and progenitor cells."
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