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"However, MYSM1 knockdown did not influence DOX-induced 𝛾 -H2AX expression (Fig. S4C), suggesting that DNA damage might not be one of the pathological conditions in MYSM1-mediated cardiotoxicity.Analogous to the loss-of-function assessment of MYSM1, the gain-of-function of MYSM1 was evaluated by transfecting HL-1 cells with Flag-MYSM1 plasmid (oeMYSM1, Fig. S4D)."