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ROBO1 binds USP33. 10 / 34

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"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

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"Robo1-USP33 interaction was confirmed in transfected cells and in embryonic neurons."

19684588

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"Slit treatment did not affect Robo1-USP33 interaction."

19684588

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"To test the specificity of Robo1-USP33 interaction, we examined whether Robo1 was co-immunoprecipitated with USP20, a homolog sharing similar domain organization and 59% amino acid identity with USP33 xref ."

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"We confirmed that the endogenously expressed Robo1 and USP33 interacted with each other in the mouse brain ( xref )."

19684588

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"In summary, our experiments demonstrate that USP33 interacts with the intracellular domain of Robo1 and that USP33 is required for Slit responsiveness of commissural neurons."

19684588

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"USP33 is associated with the axonal guidance receptor Roundabout (Robo) 1 ( xref ), which plays a major role in controlling axon crossing across the midline between brain hemispheres and neuronal dendrites ( xref )."

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"USP33 interacts with Robo1 and mediates Slit2 signaling in CRC cells."

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"Ubiquitin-specific protease 33 (USP33)/VDU1 was also shown to be involved in ROBO1–USP33 interaction and participate in SLIT/ROBO signaling in cancer cell migration. xref Another study also showed that srGAP1 is an important downstream molecule of Slit2 signaling in CRC, and mediates the antimigration function of Slit2 by inhibiting Cdc42. xref SrGAP2 protein expression is reduced or absent in a subset of primary osteosarcoma samples, srGAP2 and other axon guidance proteins likely play a role in osteosarcoma metastasis. xref "

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"Our current work shows that in lung cancer cells, USP33 also interacts with Robo1 and is required for Slit signaling in inhibiting lung cancer cell migration."

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CRC binds ROBO1 and USP33. 2 / 2

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"In CRC cells, USP33 interacts with Robo1 and mediates the inhibitory activity of Slit2 on CRC cell migration by deubiquitinating and stabilizing Robo1."

25242263

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"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

25242263

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

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"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

ROBO1 binds HTATIP2 and USP33. 1 / 1

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"Co-immunoprecipitation using deletion mutants of Robo1 and USP33 showed that the Robo1 CC3 motif interacted with USP33 in the region containing the catalytic USP and substrate-recognizing DUSP domains xref ( xref )."

19684588

USP33 deubiquitinates ROBO1.

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USP33 deubiquitinates ROBO1. 8 / 9

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"Moreover, USP33 could deubiquitinate and stabilize Robo1 and was responsible for the redistribution of Robo1 to the plasma membrane [42]."

36582601

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"USP33 deubiquitinates and stabilizes Robo1."

25242263

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"These results show that USP33 mediates Slit-Robo signaling by deubiquitinating and stabilizing Robo1 in CRC cells (XREF_SUPPLEMENTARY)."

25242263

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"USP33 mediates Slit2 signaling by deubiquitinating and stabilizing Robo1."

25242263

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"Cell based assays support a model in which USP33 reverses Robo1 ubiquitination : knockdown of USP33 increases levels of ubiquitinated Robo1, while USP33 overexpression reduces ubiquitinated Robo1 levels [XREF_BIBR]."

21704388

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"Though direct deubiquitination of Robo1 by USP33 has not been reported, it is reasonable to speculate that USP33 deubiquitinates and thus rescues Robo1 from degradation during the Slit response."

21704388

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"In addition, Xia et al. demonstrated that USP33 deubiquitinates and stabilizes ROBO1, which is required for the SLIT2-mediated inhibition of EMT and GC cell metastasis [57]."

36497313

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"USP33 deubiquitinates ROBO1 in lung cancer cells to maintain its stability, thereby regulating SLIT2 activity and inhibiting cancer cell metastasis [XREF_BIBR]."

29743814

Modified USP33 leads to the deubiquitination of ROBO1. 1 / 1

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"Overexpression of wild-type USP33, but not catalytically inactive C163A mutant of USP33, decreased the ubiquitination of Robo1."

24981056

USP33 increases the amount of ROBO1.

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USP33 increases the amount of ROBO1. 2 / 2

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"Knocking-down USP33 in H1299 cells increased the level of ubiquitinylated Robo1 in the presence of MG132, an inhibitor of the ubiquitin proteasome system."

24981056

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"USP33 silencing in A549 and SPC-A-1 cells significantly promoted the cell migration, invasion and metastasis and obviously down-regulated the expressions of SLIT2 and ROBO1."

30187867

Modified USP33 increases the amount of ubiquitinated ROBO1. 1 / 1

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"USP33 knockdown increased the ubiquitinated Robo1 level, whereas overexpression of GFP-USP33 reduced the ubiquitinated Robo1 level (XREF_SUPPLEMENTARY), suggesting that USP33 play a role in Robo1 deubiquitination."

19684588

Modified USP33 increases the amount of ROBO1. 1 / 1

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"Conversely, overexpression of wild-type USP33, not the C163A mutant USP33, in H1299 cells increased the levels of Robo1."

24981056

USP33 inhibits ROBO1.

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USP33 inhibits ROBO1. 2 / 3

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"Downregulation of USP33 reduces the protein stability of Robo1 in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer cells."

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ARRB2 binds USP33. 10 / 23

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"In addition, we established that D 3 R desensitization conditions induce the interaction between arrestin2 and USP33 (Fig. S3C)."

36658650

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"Thus, isoproterenol stimulation induces a reciprocal pattern of USP33 interaction with the β 2 AR and β-arrestin2: dissociation of USP33 from the β 2 AR and association of USP33 with β-arrestin2."

23764054

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"The immunoprecipitation assay showed that USP33 can interact with β-arrestin2 in an agonist-dependent manner (Figure xref )."

27835898

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"Upon beta 2 AR activation, the recruited beta-arrestins are rapidly ubiquitinated and deubiquitinated, and this is facilitated by a high-affinity interaction between beta-arrestin2 and USP33."

21680031

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"The immunoprecipitation assay showed that USP33 can interact with beta-arrestin2 in an agonist dependent manner."

27835898

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"This chapter focuses on the interaction of β-arrestin2 and USP33 and describes the assays used to determine the specific effects of USP33 on the trafficking of β-arrestin2 and on ERK signaling."

24377933

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"While examining the kinetics of agonist-dependent association of endogenously expressed USP33 with β-arrestin2, characteristic binding patterns were revealed ()."

24377933

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"Upon β 2 AR activation, the recruited β-arrestins are rapidly ubiquitinated and deubiquitinated, and this is facilitated by a high-affinity interaction between β-arrestin2 and USP33."

21680031

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"Upon stimulation of the βAR with isoproterenol, a robust increase in the association of β-arrestin2 and USP33 was detected."

24377933

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"At a molecular level, these effects are dependent upon conformation-specific interaction of USP33 and β-arrestin2."

23764054

AR binds ARRB2 and USP33. 1 / 1

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23764054

USP33 deubiquitinates ARRB2.

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USP33 deubiquitinates ARRB2. 6 / 7

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"Beta-arrestin-2 can be ubiquitinated by Mdm2 (see above) and deubiquitinated by the DUB enzyme USP33."

20854416

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"Importantly, a knockdown of USP20 and USP33 enhances beta-arrestin2 ubiquitination and increases beta 2 AR degradation [XREF_BIBR, XREF_BIBR]."

24680432

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"Further results verified that USP33 can deubiquitinate beta-arrestin2, subsequently block the internalization of SDF-1-stimulated CXCR4, and disrupt beta-arrestin-dependent ERK activation."

27835898

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"USP33 leads to de-ubiquitination of beta-arrestin2 with non degradative effects."

27835898

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"Given that the enzymatic activity of USP33 is important in regulating CXCR4 internalization, we were interested in whether USP33 can deubiquitinate beta-arrestin2 and subsequently regulate CXCR4 endocytosis."

27835898

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"Subsequent deubiquitination of beta-arrestin 2 by the deubiquitinase USP33 dissociates the receptor, beta, and arrestin complex."

19853559

USP33 ubiquitinates ARRB2.

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USP33 leads to the ubiquitination of ARRB2. 2 / 2

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USP33 binds CCP110. 6 / 13

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"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain ( xref )."

23486064

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"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."

23486064

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"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays (XREF_SUPPLEMENTARY) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 (XREF_SUPPLEMENTARY)."

23486064

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"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain (XREF_FIG)."

23486064

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"Alternatively, excessive amounts of USP33-DM might bind CP110, blocking it from accessing SCF Cyclin F."

23486064

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"USP33 interacts with CP110 and localizes to centrioles primarily during S and G2/M phase, the period during which centrioles duplicate and elongate."

23486064

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

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"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

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"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."

23486064

USP33 activates CCP110.

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USP33 activates CCP110. 4 / 8

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"Further, USP33 suppression significantly reduced CP110 protein half-life from ~ 4.5 hours to ~ 2 hours (XREF_SUPPLEMENTARY)."

23486064

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"We observed that USP33 depletion did not lead to a reduction in RRM2 levels (XREF_SUPPLEMENTARY), indicating that USP33 specifically stabilizes CP110."

23486064

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"USP33 promotes the generation of excessive centriolar foci due to elevated levels of CP110 because (1) over-expression of human or murine USP33 led to CP110 up-regulation in multiple cell lines (XREF_SUPPLEMENTARY) and (2) depletion of CP110 overrode USP33 mediated generation of centriolar foci (XREF_SUPPLEMENTARY)."

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"Further, depletion of USP33 in two PDAC cell lines (Panc1 and MiaPaCa2) led to a reduction in CP110 levels (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY), indicating that USP33 stabilizes CP110 in PDAC cells."

23486064

USP33 deubiquitinates CCP110.

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USP33 deubiquitinates CCP110. 4 / 5

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"For example, USP33 deubiquitylates the centrosomal protein CP110, regulating centrosome biogenesis [76] and USP33 depletion has a co-operative effect with CDK2 on the CP110-dependent clustering function mentioned earlier [77]."

31506331

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"USP33 deubiquitinates the CP110 protein, an important regulator of centrosome duplication ( Li et al., 2013 )."

25444757

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"Thus, USP33 specifically de-ubiquitylates CP110 in vitro and in vivo, explaining how USP33 stabilizes CP110."

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USP33 activates cell population proliferation. 10 / 18

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"Function experiments revealed that USP33 overexpression promoted the proliferation, migration and invasion of PC cells while the inhibition of USP33 expression in PC cells exhibited the opposite effect."

37322017

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"In conclusion, our study found that USP33 contributed to the proliferation and metastasis of pancreatic cancer through a positive feedback loop with TGF-beta signaling pathway."

37322017

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"Next, functional rescue assays were performed to further verify that the miR-365a-3p-mediated downregulation of USP33 promotes the proliferation, migration, and invasion of lung cancer cells."

29743814

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"Functionally, our experiments demonstrate that USP33 significantly enhances ovarian cancer proliferation and metastasis in a CBX2-dependent manner."

39256572

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"Additionally, we confirmed that 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) was critical for USP33-induced proliferation and aerobic glycolysis in OS cells, and the protein expression levels of PFKFB3 and USP33 were positively correlated in the OS tissues."

37034227

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"Through our function experiments, we found that USP33 promoted the proliferation, migration and invasion of PC in vitro and in vivo."

37322017

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"Previous studies showed that USP33 promotes cell proliferation and reduces apoptosis in retinoblastoma [ 36 ]."

37267862

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"The overexpression of USP33 accelerated the proliferation, invasion and metastasis of PC in vitro and in vivo."

37322017

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"USP33 promoted the proliferation, migration and invasion of PC cells."

37322017

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"Moreover, overexpression of USP33 Cys : His can slightly promote cell proliferation and invasion (XREF_SUPPLEMENTARY), instead of the inhibiting effects caused by USP33 wild type."

27835898

USP33 inhibits cell population proliferation.

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USP33 inhibits cell population proliferation. 10 / 12

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"Cellular studies with TPC-1 and BCPAP cells demonstrated that USP33 can attenuate the cell capacities of proliferation, migration and invasion."

29533940

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"Furthermore, we found that beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and cell invasion."

28506875

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"Cell experiments showed that USP33 overexpression significantly inhibited the proliferation, migration, and invasion of GAC cells."

29802710

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"USP33 inhibits cell proliferation, migration, and invasion."

27835898

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"Additionally, USP33 overexpression suppresses the proliferation, migration, and invasion of gastric adenocarcinoma cells [56]."

36497313

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"USP33 knockdown significantly upregulated tumor cell proliferation and metastasis."

29802710

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"These results indicate that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation."

28506875

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"Taken together, we found that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation and invasion.So far, the study of USP33 is still very limited."

28506875

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"Overexpression of USP33 can significantly inhibit cell proliferation, migration, and invasion."

27835898

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"Our data showed that knockdown of USP33 promoted the proliferation of both HeLa and HCT116 cells."

28506875

BTRC affects USP33

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BTRC binds USP33.

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BTRC binds USP33. 10 / 13

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"We found that USP33 (^ DSG) can also interact with beta-TrCP."

28506875

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"Β-TrCP can also interact with FH-USP33(△DSG) ( Fig. 3 B ), indicating that the interaction between β-TrCP and USP33 is independent of DSG motif of USP33."

28506875

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"The interaction of β-TrCP with these USP33 mutants was evaluated."

28506875

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"Β-TrCP is able to interact with USP33, and promotes the ubiquitination and proteasomal degradation of USP33."

28506875

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"Our data showed that USP33 was able to interact with β-TrCP-WT, but unable to interact with β-TrCP-△WD40 ( Fig. 3 D )."

28506875

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"Collectively, we found that the WD40 motif of β-TrCP and 201–400 amino acid motif of USP33 are necessary for the interaction between β-TrCP and USP33."

28506875

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"We found that USP33(△DSG) can also interact with β-TrCP ( Fig. 3 B )."

28506875

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"Since β-TrCP promotes USP33 ubiquitin-dependent degradation, then we assessed the interaction between β-TrCP and USP33."

28506875

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"These results suggest that the effect of beta-TrCP on USP33 is independent of the classic DSG motif.Since beta-TrCP promotes USP33 ubiquitin dependent degradation, then we assessed the interaction bet[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

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"Beta-TrCP can also interact with FH-USP33 (^ DSG), indicating that the interaction between beta-TrCP and USP33 is independent of DSG motif of USP33."

28506875

BTRC activates USP33.

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BTRC activates USP33. 8 / 8

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"These results indicate that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation."

28506875

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"These data suggest that beta-TrCP might mediate USP33 downregulation at the protein level."

28506875

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"Our results showed that MG132 blocked beta-TrCP-mediated USP33 downregulation, suggesting that beta-TrCP promotes USP33 downregulation via the proteasome pathway."

28506875

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"The results showed that knockdown of beta-TrCP in cells slowed down the degradation rate of endogenous USP33 and therefore enhanced USP33 protein stability."

28506875

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"Taken together, these results suggest that beta-TrCP downregulates USP33 expression at the protein level but not at the mRNA level.To uncover the mechanism of USP33 downregulation mediated by beta-TrC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

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"Furthermore, we found that beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and cell invasion."

28506875

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"Taken together, we found that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation and invasion.So far, the study of USP33 is still very limited."

28506875

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"Furthermore, we demonstrated that overexpression of beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and invasion."

28506875

BTRC ubiquitinates USP33.

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BTRC leads to the ubiquitination of USP33. 2 / 2

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"Furthermore, beta-TrCP also increased the ubiquitination of USP33 (^ DSG) and decreased the protein stability of USP33 (^ DSG)."

28506875

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"Furthermore, β-TrCP also increased the ubiquitination of USP33(△DSG) ( Fig. 2 D ) and decreased the protein stability of USP33(△DSG) ( Fig. 4 B )."

28506875

Modified BTRC leads to the ubiquitination of USP33. 1 / 1

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"The results showed that overexpression of beta-TrCP substantially increased the poly-ubiquitination of endogenous USP33."

28506875

BTRC decreases the amount of USP33.

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BTRC decreases the amount of USP33. 2 / 3

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28506875

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"In contrast, knockdown of beta-TrCP in HEK293T cells upregulated endogenous USP33 protein levels."

28506875

BTRC inhibits USP33.

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BTRC inhibits USP33. 2 / 2

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"Thus, our data revealed a new regulatory mechanism of the deubiquitinase USP33, and uncovered an additional signaling pathway, that is, beta-TrCP downregulates USP33 to promote cell proliferation.HeLa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

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"The results showed that knockdown of beta-TrCP in cells slowed down the degradation rate of endogenous USP33 and therefore enhanced USP33 protein stability."

28506875

USP33 affects Neoplasm Invasiveness

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USP33 activates Neoplasm Invasiveness.

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USP33 activates Neoplasm Invasiveness. 10 / 19

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"USP33 promoted the proliferation, migration and invasion of PC cells."

37322017

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"As shown in Fig. 3A, compared with other ECM components, the binding ability of USP33-overexpressed cells was significantly increased in the laminin (which is also the primary component of Matrigel) group, indicating that USP33-mediated invasion may be related to the inducement of the adhesiveness to laminin."

39589547

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"USP33 promotes cell invasion, but not growth and migration, in normal cell culture conditions."

39589547

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"Next, functional rescue assays were performed to further verify that the miR-365a-3p-mediated downregulation of USP33 promotes the proliferation, migration, and invasion of lung cancer cells."

29743814

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"As expected, knockdown of USP33 only attenuated KYSE-150 cell invasion (Fig. 2F)."

39589547

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"Together, our results indicate that USP33 can promote cell invasion but has no remarkable effect on cell proliferation and migration in normal cell culture conditions."

39589547

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"Since cell-matrix adhesion is closely correlated with cell migration and invasive potentials in tumors (Hamidi and Ivaska 2018; Chastney et al. 2024), we wondered whether USP33-mediated invasion is specific ECM component(s) dependent."

39589547

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"Our results reveal that USP33 acts as the deubiquitinating enzyme of SP1 and contributes to HCC invasion and metastasis through activation of the SP1 / c-Met axis ."

32835698

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"This is the first evidence for a lung cancer invasion and metastasis mechanism in which miR-365a-3p directly targets and downregulates USP33, which further promotes the proliferation, migration, and invasion activities of lung cancer."

29743814

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"Through our function experiments, we found that USP33 promoted the proliferation, migration and invasion of PC in vitro and in vivo."

37322017

USP33 inhibits Neoplasm Invasiveness.

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USP33 inhibits Neoplasm Invasiveness. 9 / 9

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"Cellular studies with TPC-1 and BCPAP cells demonstrated that USP33 can attenuate the cell capacities of proliferation, migration and invasion."

29533940

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"Cell experiments showed that USP33 overexpression significantly inhibited the proliferation, migration, and invasion of GAC cells."

29802710

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reach

"Furthermore, we found that beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and cell invasion."

28506875

➶

reach

"USP33 inhibits cell proliferation, migration, and invasion."

27835898

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reach

"Additionally, USP33 overexpression suppresses the proliferation, migration, and invasion of gastric adenocarcinoma cells [56]."

36497313

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"USP33 silencing can promote the migration, invasion and metastasis of lung adenocarcinoma cells in vitro, and the mechanism may involve IL-6 and SLIT2 and ROBO1 signaling pathways."

30187867

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"Overexpression of USP33 can significantly inhibit cell proliferation, migration, and invasion."

27835898

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"[USP33 suppresses lung adenocarcinoma lung cell invasion and metastasis by down-regulating SLIT2 and ROBO1 signaling pathway]."

30187867

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"Furthermore, we demonstrated that overexpression of beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and invasion."

28506875

ARRB2 affects USP33

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ARRB2 binds USP33. 10 / 23

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"In addition, we established that D 3 R desensitization conditions induce the interaction between arrestin2 and USP33 (Fig. S3C)."

36658650

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23764054

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"The immunoprecipitation assay showed that USP33 can interact with β-arrestin2 in an agonist-dependent manner (Figure xref )."

27835898

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"Upon beta 2 AR activation, the recruited beta-arrestins are rapidly ubiquitinated and deubiquitinated, and this is facilitated by a high-affinity interaction between beta-arrestin2 and USP33."

21680031

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reach

"The immunoprecipitation assay showed that USP33 can interact with beta-arrestin2 in an agonist dependent manner."

27835898

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sparser

"This chapter focuses on the interaction of β-arrestin2 and USP33 and describes the assays used to determine the specific effects of USP33 on the trafficking of β-arrestin2 and on ERK signaling."

24377933

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"While examining the kinetics of agonist-dependent association of endogenously expressed USP33 with β-arrestin2, characteristic binding patterns were revealed ()."

24377933

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sparser

"Upon β 2 AR activation, the recruited β-arrestins are rapidly ubiquitinated and deubiquitinated, and this is facilitated by a high-affinity interaction between β-arrestin2 and USP33."

21680031

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sparser

"Upon stimulation of the βAR with isoproterenol, a robust increase in the association of β-arrestin2 and USP33 was detected."

24377933

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"At a molecular level, these effects are dependent upon conformation-specific interaction of USP33 and β-arrestin2."

23764054

AR binds ARRB2 and USP33. 1 / 1

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23764054

VHL affects USP33

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VHL binds USP33.

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VHL binds USP33. 10 / 19

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"The USP33-VHL interaction may have clinical significance because naturally occurring mutations that have been found in the beta-domain of USP33 disrupt the interaction between the two proteins."

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"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."

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"Finally, we demonstrate that VDU1 is able to be ubiquitinated via a pVHL dependent pathway for proteasomal degradation, and VHL mutations that disrupt the interaction between VDU1 and pVHL abrogate the ubiquitination of VDU1."

11739384

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"We have determined that the VDU1-interacting region in pVHL is located in its beta-domain, and several naturally occurring mutations located in this domain disrupt the interaction between pVHL and VDU1 protein."

11739384

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"We propose a model, in which hSP56 may function as a novel regulator of ubiquitination-dependent protein degradation pathways by altering the enzymatic activity of VDU1 or by regulating the interactio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19118533

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"Interestingly, several naturally occurring mutations in the VDU-binding domain of pVHL were found to disrupt the interaction between VDU1/VDU2 and pVHL, thus suggesting a functional relevance in pVHL-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

17961905

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"Finally, we demonstrate that VDU1 is able to be ubiquitinated via a pVHL-dependent pathway for proteasomal degradation, and VHL mutations that disrupt the interaction between VDU1 and pVHL abrogate the ubiquitination of VDU1."

5 | 15 3

USP33 binds TGFBR2.

5 | 1 3

USP33 binds TGFBR2. 3 / 8

5 | 3

➶

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"Our previous experiments proved that USP33 interacted with TGFBR2, while the effect of USP33 in TGFBR2 was unclear."

37322017

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"Mechanistically, USP33 interacted with TGFBR2 through DUSP1 domain of USP33, moreover, USP33 enhanced the protein stability of TGFBR2 by removing the K63-linked ubiquitin chains on TGFBR2."

37322017

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"Further Co-IP experiments showed a similar result with our luciferase complementation assay, TGFBR2 and USP33 could bind to each other while the other candidates had no interaction with USP33 (Fig. xref )."

37322017

USP33 binds TGFBR2 and DUSP1 domain. 1 / 1

| 1

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"All in all, our data revealed that USP33 regulated the K63 deubiquitination of TGFBR2 and promoted the its protein stability, meanwhile, the binding between USP33 and TGFBR2 was dependent on the DUSP1 domain of USP33."

37322017

USP33 activates TGFBR2.

| 6

USP33 activates TGFBR2. 6 / 6

| 6

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"The IF assay showed that after the overexpression of USP33, the colocalization of TGFBR2 with lysosome was impaired, meanwhile USP33 overexpression promoted the accumulation of TGFBR2 in cell membrane (Fig. 5K)."

37322017

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reach

"Moreover, our data showed that USP33 promoted the recycling of TGFBR2 to cell membrane in PC cells during its internalization (Fig. S2)."

37322017

➶

reach

"In conclusion, our study suggested that USP33 prevented the degradation of TGFBR2 by lysosome by promote its recycling by the recycling endosome."

37322017

➶

reach

"Mechanistically, USP33 interacted with TGFBR2 through DUSP1 domain of USP33, moreover, USP33 enhanced the protein stability of TGFBR2 by removing the K63-linked ubiquitin chains on TGFBR2."

37322017

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reach

"Mechanistically, USP33 deubiquitinated and stabilized the TGFBR2 protein in PC cells and therefore enhanced the signaling of TGF-β pathway, USP33 removed the K63-linked ubiquitin conjugates from TGFBR2 and prevented its degradation by lysosome, meanwhile, USP33 promoted the recycling of TGFBR2 to cell membrane and eventually enhanced the signaling in TGF-β pathway."

37322017

➶

reach

"USP33 promoted the recycling of TGFBR2."

37322017

USP33 deubiquitinates TGFBR2.

| 4

USP33 deubiquitinates TGFBR2. 4 / 4

| 4

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reach

"Liu et al. reported that USP33 expression was increased among PC samples, which was correlated with poor survival of patients with PC; they found that USP33 could promote PC cell growth, migration, and invasion by triggering the deubiquitination of TGFBR2 (Liu et al. 2023)."

39589547

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"The USP33-regulated deubiquitination of TGFBR2 avoided its lysosomal degradation, meanwhile USP33 promoted the colocalization of TGFBR2 with recycling endosome which led to its accumulation on the membrane and the activation of TGFβ signaling."

37322017

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reach

"Mechanistically, USP33 triggered the deubiquitination of TGFBR2 and prevented its degradation by lysosome, therefore promoted TGFBR2 accumulation in cell membrane and eventually contributed to the sustained activation of TGF-beta signaling."

37322017

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reach

"Our results demonstrated that the K63R-Ub mutant and USP33 co-transfection had no effect on TGFBR2 ubiquitination, while the co-transfection of USP33 with other Ub mutants impaired the ubiquitination of TGFBR2 (Fig. 6E)."

37322017

USP33 increases the amount of TGFBR2.

| 2

USP33 increases the amount of TGFBR2. 2 / 2

| 2

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reach

"Our results suggested that USP33 knockdown inhibited the expression of TGFBR2 protein, the overexpression of USP33 had the opposite effect on TGFBR2 protein while no alteration was observed in TGFBR2 mRNA after the manipulation on USP33 expression (Fig. 5E–G), all these results indicated that USP33 may participate in the posttranslational modification of TGFBR2."

2 | 6 12

USP20 binds USP33. 10 / 13

2 | 6 4

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"Two DUBs, USP33 (or VDU-1) and USP20 (or VDU-2), interact with and regulate D2 [ xref ]."

29892818

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17961905

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"In the current studies and together with our previous report [30] , we have demonstrated that both VDU1 and VDU2 also bind to the β-domain of pVHL and several naturally occurring mutations in the β-d[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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"Moreover, pVHL30 is also known to interact with both USP33 and USP20, two enzymes belonging to the USP protein family, whose USP7 is a further member [ xref ]."

35205757

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sparser

"USP33 and USP20 likely interact with the ER membrane bilayer via their amphipathic helices since they do not have transmembrane domains [ xref ]."

35357021

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"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."

38874156

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12056827

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12056827

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23486064

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12056827

GroEL2 binds USP33 and USP20. 4 / 4

| 4

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"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

24874852

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sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

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"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."

24874852

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

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sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

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VHL binds USP33 and USP20. 1 / 1

| 1

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"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

12056827

ARs binds USP33 and USP20. 1 / 1

| 1

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sparser

"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."

23764054

USP20 activates USP33.

| 1

USP20 activates USP33. 1 / 1

| 1

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"The closely related DUBs, USP20 and USP33, deubiquitylate activated beta-adrenergic receptors as well as the adaptor and signalling protein beta-arrestin (in the case of USP33), to favour recycling from endosomes, despite largely being associated with the secretory pathway 75, 76, 77."

28064438

USP33 affects VHL

6 1 | 7 7

USP33 binds VHL.

6 1 | 6 7

VHL binds USP33. 10 / 19

6 1 | 6 6

➶

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"The USP33-VHL interaction may have clinical significance because naturally occurring mutations that have been found in the beta-domain of USP33 disrupt the interaction between the two proteins."

18687060

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12056827

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reach

12056827

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12056827

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19571111

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11739384

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11739384

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19118533

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17961905

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17961905

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12056827

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"Moreover, pVHL30 is also known to interact with both USP33 and USP20, two enzymes belonging to the USP protein family, whose USP7 is a further member [ xref ]."

35205757

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sparser

"USP33 and USP20 likely interact with the ER membrane bilayer via their amphipathic helices since they do not have transmembrane domains [ xref ]."

35357021

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38874156

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12056827

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12056827

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23486064

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12056827

GroEL2 binds USP33 and USP20. 4 / 4

| 4

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sparser

24874852

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"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

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sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

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24874852

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

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sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

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VHL binds USP33 and USP20. 1 / 1

| 1

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sparser

"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

12056827

ARs binds USP33 and USP20. 1 / 1

| 1

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"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."

23764054

USP33 affects Neoplasm Metastasis

| 1 20

USP33 activates Neoplasm Metastasis.

| 1 17

USP33 activates Neoplasm Metastasis. 10 / 18

| 1 17

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"For example, a recent study revealed that enhanced USP33 expression was associated with reduced survival rates in OC patients; mechanistically, USP33 promotes OC cell growth and metastasis via deubiquitinating and stabilizing CBX2 (Chen et al. 2024)."

39589547

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reach

"Functionally, our experiments demonstrate that USP33 significantly enhances ovarian cancer proliferation and metastasis in a CBX2-dependent manner."

39256572

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reach

"In conclusion, our study found that USP33 contributed to the proliferation and metastasis of pancreatic cancer through a positive feedback loop with TGF-beta signaling pathway."

37322017

➶

reach

"USP33 is an integrin alpha6 deubiquitinase and promotes esophageal squamous cell carcinoma cell migration and metastasis."

39589547

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reach

"For example, in hepatocellular carcinoma (HCC), the deubiquitinase USP33 stabilizes Sp1, leading to increased c-Met expression and enhanced HCC metastasis (29)."

40125551

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reach

"We found that USP33 knockdown inhibited the invasion and metastasis in HCC cells both in vitro and in vivo, which was partly dependent on c-Met."

32835698

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reach

"Our results reveal that USP33 acts as the deubiquitinating enzyme of SP1 and contributes to HCC invasion and metastasis through activation of the SP1 and c-Met axis."

32835698

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reach

"USP33/TGFBR2 axis promoted the tumor growth and metastasis of PC in vivo."

37322017

➶

reach

"The overexpression of USP33 accelerated the proliferation, invasion and metastasis of PC in vitro and in vivo."

37322017

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reach

"Additionally, results from in vivo and in vitro studies showed that USP33 knockdown inhibits invasion, migration, and metastasis in HCC cells."

35924961

USP33 inhibits Neoplasm Metastasis.

| 3

USP33 inhibits Neoplasm Metastasis. 3 / 3

| 3

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"USP33 silencing can promote the migration, invasion and metastasis of lung adenocarcinoma cells in vitro, and the mechanism may involve IL-6 and SLIT2 and ROBO1 signaling pathways."

30187867

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"USP33 knockdown significantly upregulated tumor cell proliferation and metastasis."

29802710

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"[USP33 suppresses lung adenocarcinoma lung cell invasion and metastasis by down-regulating SLIT2 and ROBO1 signaling pathway]."

30187867

USP33 affects MYC

2 1 | 11 5

USP33 binds MYC.

2 | 6 5

USP33 binds MYC. 10 / 13

2 | 6 5

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"IP experiments showed that USP33 interacted with c-Myc ( xref ), and USP33 knockdown profoundly enhanced c-Myc ubiquitination ( xref )."

33718387

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sparser

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

sparser

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

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sparser

"USP33 can bind to c-Myc."

36966306

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reach

"USP33 could bind and deubiquitinate c-Myc."

33718387

➶

reach

"IP experiments showed that USP33 interacted with c-Myc (XREF_FIG), and USP33 knockdown profoundly enhanced c-Myc ubiquitination (XREF_FIG)."

33718387

➶

reach

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

reach

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

reach

"USP33, as a deubiquitinating enzyme, can bind and deubiquitinate c-Myc, thereby promoting the proliferation and cell cycle progression of PCa cells and reducing their sensitivity to DTX [70]."

40232412

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reach

"USP33 can bind to c-Myc."

36966306

USP33 increases the amount of MYC.

| 3

USP33 increases the amount of MYC. 2 / 2

| 2

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"In addition, the protein levels of α-SMA, COL1A2, LDHA, and c -Myc were significantly reduced by Usp33 knockdown in primary hepatic stellate cells ( Fig. 3 F and G)."

37267862

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"Here, our data showed knockdown of USP33 significantly decreased ECAR, the release of lactic acid, ATP synthase, and expression of c -Myc and LDHA in primary hepatic stellate cells, suggesting the gly[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37267862

USP33 increases the amount of MYC. 1 / 1

| 1

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reach

"USP33 knockdown significantly reduced c-Myc protein expression (XREF_FIG), but did not affect c-Myc mRNA expression (XREF_FIG)."

33718387

USP33 deubiquitinates MYC.

1 | 2

USP33 deubiquitinates MYC. 2 / 3

1 | 2

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"USP33, as a deubiquitinating enzyme, could deubiquitinate c-Myc, an important proliferation regulator."

33718387

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reach

"IP experiments showed that USP33 interacted with c-Myc (XREF_FIG), and USP33 knockdown profoundly enhanced c-Myc ubiquitination (XREF_FIG)."

33718387

CCP110 affects USP33

3 | 6 3

CCP110 binds USP33.

3 | 5 3

USP33 binds CCP110. 6 / 13

3 | 5 1

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"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain ( xref )."

23486064

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23486064

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23486064

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"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain (XREF_FIG)."

23486064

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"Alternatively, excessive amounts of USP33-DM might bind CP110, blocking it from accessing SCF Cyclin F."

23486064

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reach

"USP33 interacts with CP110 and localizes to centrioles primarily during S and G2/M phase, the period during which centrioles duplicate and elongate."

23486064

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

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"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

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23486064

CCP110 inhibits USP33.

| 1

CCP110 inhibits USP33. 1 / 1

| 1

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"We found that centrosomal targeting of USP33 is partially dependent on CP110 because depletion of CP110 reduced USP33 localization to centrosomes (see below) (XREF_SUPPLEMENTARY)."

23486064

USP33 affects cell migration

| 14

USP33 activates cell migration.

| 11

USP33 activates cell migration. 10 / 12

| 11

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"Interestingly, overexpression of USP33 significantly increased the cell migration (Fig. 3C) but not the proliferation (Fig. 3B) ability upon laminin coating."

39589547

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"These findings are the first to describe that USP33 promoted laminin-dependent cell migration by stabilizing integrin α6, which provides an insight into the mechanistic roles of USP33-integrin α6 axis in ESCC metastasis."

39589547

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"Deficiency of HIF1A or USP33 suppressed cell migration, proliferation and microtubule formation of hypoxia-exposed HRECs."

39320536

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"Expression of the C163A-mutant USP33 abolished Slit suppression of H1299 cell migration, supporting that deubiquitinating activity of USP33 is important in mediating Slit activity in inhibiting lung cancer cell migration."

24981056

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"Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT."

38487955

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"Moreover, we demonstrated that USP33 could mediate ESCC cell migration and metastasis through integrin α6."

39589547

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reach

"USP33 is an integrin alpha6 deubiquitinase and promotes esophageal squamous cell carcinoma cell migration and metastasis."

39589547

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"Mechanistically, USP33 mediates cell migration through binding, deubiquinating, and stabilizing integrin alpha6."

39589547

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reach

"USP33 knockdown could inhibit ESCC cell migration and metastasis majorly through integrin alpha6."

39589547

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"We found that integrin α6 and USP33 overexpression in USP33-knockdown cells can largely rescue cell migration abilities on laminin in both KYSE-150 and KYSE-450 cells compared to indicated knockdown cells (Fig. 5C), which highlights the importance of integrin α6 in USP33-mediated ESCC cell migration on laminin."

39589547

USP33 inhibits cell migration.

| 3

USP33 inhibits cell migration. 3 / 3

| 3

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"As examples, USP33 was down-regulated in lung cancer patients and correlated with poor prognosis, inhibiting lung cancer cell migration through stabilization of the Robo1 protein (Wen et al. 2014)."

39589547

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"In addition, USP33 also inhibits breast cancer cell migration via regulating Slit signaling [ 35 ]."

37267862

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"Similarly, the downregulation of USP33 (a ubiquitin ligase enzyme) reduces the stability of the ROBO1 receptor in lung cancer cells 29 and breast cancer cell migration 30."

33318575

GroEL2 affects USP33

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GroEL2 binds USP33. 10 / 10

| 6 4

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"We then examined the interaction of the full-length VDU1 and hSP56 without or with pre-incubation of hSP56 with selenium."

19118533

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"Notably, the results indicate that the interaction between hSP56 and VDU1 is direct, since purified proteins were used ( Fig. 1 B and C)."

19118533

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"The results indicate that the selenium is required for the hSP56 and VDU1 interaction.We compared selenium binding to hSP56 in non reducing or reducing SDS-PAGE to determine the nature of the interact[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19118533

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"The full-length VDU1 interacts specifically with the selenium-replete form of hSP56 (hSP56/Se) in vitro , strongly suggesting that a physiological partner of VDU1 may be hSP56/Se ( Fig. 2 C)."

19118533

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reach

"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA and anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm (XREF_FIG D)."

24874852

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"HSP56 interacts with both VDU1 and VDU2."

24874852

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"HSP56 interacted strongly with VDU1 f , compared to no interaction with GST alone or a trivial interaction with GST-β2m ( Fig. 1 B)."

19118533

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"HSP56 interacted strongly with VDU1 f, compared to no interaction with GST alone or a trivial interaction with GST-beta2m."

19118533

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reach

"Notably, the results indicate that the interaction between hSP56 and VDU1 is direct, since purified proteins were used."

19118533

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"HSP56 and VDU1 were present together in the cytoplasm as demonstrated in the merged images, consistent with physiologically relevant interactions.We then examined the interaction of the full-length VD[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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GroEL2 binds USP33 and USP20. 4 / 4

| 4

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24874852

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"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

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sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

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24874852

USP33 affects Pancreatic Neoplasms

| 13

USP33 activates Pancreatic Neoplasms.

| 12

USP33 activates Pancreatic Neoplasms. 10 / 12

| 12

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"Through our function experiments, we found that USP33 promoted the proliferation, migration and invasion of PC in vitro and in vivo."

37322017

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reach

"Our further experiments revealed that USP33 mediated the progression of PC cells through TGFBR2 which was a key regulator of TGFβ signaling pathway."

37322017

➶

reach

"The overexpression of USP33 accelerated the proliferation, invasion and metastasis of PC in vitro and in vivo."

37322017

➶

reach

"To assess whether USP33 affected the invasiveness and migration of PC cells, we performed the transwell migration and invasion assays and found that USP33 knockdown significantly inhibited the migration and invasiveness of PC cells while USP33 overexpression showed the opposite effect (Fig. 2H–J)."

37322017

➶

reach

"USP33/TGFBR2 axis promoted the tumor growth and metastasis of PC in vivo."

37322017

➶

reach

"USP33 promoted the proliferation, migration and invasion of PC cells."

37322017

➶

reach

"The results showed that knockdown of USP33 inhibited the liver metastasis of PC and increased the OS of PC bearing nude mice, while the overexpression of TGFBR2 remedied this phenotype (Fig. 8D–F)."

37322017

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"Moreover, we labeled EdU-positive PC cells to monitor the proliferation rate of PC cells, our data showed that EdU-positive cells was markedly reduced after USP33 downregulation while USP33 overexpression increased the proliferation rate of PC cells (Fig. 2F, G)."

37322017

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"Taking together, our results demonstrated that USP33 promoted the tumor growth and metastasis of PC in a TGFBR2-dependent manner."

37322017

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reach

"To investigate the role of TGFBR2 in USP33-mediated PC cell malignance, we performed the corresponding rescue experiments."

37322017

USP33 inhibits Pancreatic Neoplasms.

| 1

USP33 inhibits Pancreatic Neoplasms. 1 / 1

| 1

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37322017

USP33 affects BTRC

2 | 3 8

BTRC binds USP33. 10 / 13

2 | 3 8

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"We found that USP33 (^ DSG) can also interact with beta-TrCP."

28506875

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"Β-TrCP can also interact with FH-USP33(△DSG) ( Fig. 3 B ), indicating that the interaction between β-TrCP and USP33 is independent of DSG motif of USP33."

28506875

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sparser

"The interaction of β-TrCP with these USP33 mutants was evaluated."

28506875

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"Β-TrCP is able to interact with USP33, and promotes the ubiquitination and proteasomal degradation of USP33."

28506875

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sparser

"Our data showed that USP33 was able to interact with β-TrCP-WT, but unable to interact with β-TrCP-△WD40 ( Fig. 3 D )."

28506875

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"Collectively, we found that the WD40 motif of β-TrCP and 201–400 amino acid motif of USP33 are necessary for the interaction between β-TrCP and USP33."

28506875

➶

sparser

"We found that USP33(△DSG) can also interact with β-TrCP ( Fig. 3 B )."

28506875

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sparser

"Since β-TrCP promotes USP33 ubiquitin-dependent degradation, then we assessed the interaction between β-TrCP and USP33."

28506875

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reach

28506875

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"Beta-TrCP can also interact with FH-USP33 (^ DSG), indicating that the interaction between beta-TrCP and USP33 is independent of DSG motif of USP33."

28506875

MYC affects USP33

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USP33 binds MYC. 10 / 13

2 | 6 5

➶

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"IP experiments showed that USP33 interacted with c-Myc ( xref ), and USP33 knockdown profoundly enhanced c-Myc ubiquitination ( xref )."

33718387

➶

sparser

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

sparser

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

sparser

"USP33 can bind to c-Myc."

36966306

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reach

"USP33 could bind and deubiquitinate c-Myc."

33718387

➶

reach

"IP experiments showed that USP33 interacted with c-Myc (XREF_FIG), and USP33 knockdown profoundly enhanced c-Myc ubiquitination (XREF_FIG)."

33718387

➶

reach

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

reach

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

reach

"USP33, as a deubiquitinating enzyme, can bind and deubiquitinate c-Myc, thereby promoting the proliferation and cell cycle progression of PCa cells and reducing their sensitivity to DTX [70]."

40232412

➶

reach

"USP33 can bind to c-Myc."

36966306

TGFBR2 affects USP33

5 | 4 3

TGFBR2 binds USP33.

5 | 1 3

USP33 binds TGFBR2. 3 / 8

5 | 3

➶

sparser

"Our previous experiments proved that USP33 interacted with TGFBR2, while the effect of USP33 in TGFBR2 was unclear."

37322017

➶

sparser

"Mechanistically, USP33 interacted with TGFBR2 through DUSP1 domain of USP33, moreover, USP33 enhanced the protein stability of TGFBR2 by removing the K63-linked ubiquitin chains on TGFBR2."

37322017

➶

sparser

37322017

USP33 binds TGFBR2 and DUSP1 domain. 1 / 1

| 1

➶

reach

37322017

TGFBR2 activates USP33.

| 2

TGFBR2 activates USP33. 2 / 2

| 2

➶

reach

"Our results showed that USP33 deficiency significantly decreased the tumor growth of subcutaneous xenograft nude mice while the overexpression of TGFBR2 rescued the effect of USP33 (Fig. 8A–C)."

37322017

➶

reach

"Moreover, the transwell assay showed that TGFBR2 overexpression rescued the effect of USP33 on the migration and invasion of PC cells (Fig. 4I)."

37322017

TGFBR2 inhibits USP33.

| 1

TGFBR2 inhibits USP33. 1 / 1

| 1

➶

reach

"Moreover, our results showed that USP33 knockdown led to a decrease in the phosphorylation and translocation of SMAD2/3, while the TGFBR2 overexpression rescued the effect of USP33 knockdown (Fig. 3K)."

37322017

USP33 affects PRKN

1 | 10

USP33 deubiquitinates PRKN.

1 | 7

USP33 deubiquitinates PRKN. 3 / 4

1 | 3

➶

reach

"Cellular and in vitro assays illustrated that USP33 deubiquitinates PRKN in a DUB activity dependent manner."

31432739

➶

reach

"USP33 deubiquitinates the E3 ubiquitin‐protein ligase parkin (PRKN)."

35924961

➶

reach

"USP33 deubiquitinates PRKN and parkin and antagonizes its role in mitophagy."

31432739

USP33 deubiquitinates PRKN on K435. 4 / 4

| 4

➶

reach

"USP33 prefers to remove K6, K11, K48 and K63 linked ubiquitin conjugates from PRKN, and deubiquitinates PRKN mainly at Lys435."

31432739

➶

reach

"USP33 Deubiquitinates K63-Linked Ubiquitin on Parkin at Lys435."

36831318

➶

| 7

MiR-365a-3p activates USP33. 7 / 7

| 7

➶

reach

29743814

➶

reach

"This suggested that miR-365a-3p directly targets and binds the USP33 3 ' UTR."

29743814

➶

reach

"Functional rescue assays demonstrated that miR-365a-3p promoted proliferation, migration, and invasion by downregulating USP33."

29743814

➶

reach

"This further suggested that miR-365a-3p promotes the proliferation of lung cancer cells by downregulating USP33."

29743814

➶

reach

"The above series of in vitro experiments suggested that miR-365a-3p promotes lung cancer progression by downregulating USP33; however, the signalling pathways involved in this process remain unknown."

29743814

➶

| 2 8

➶

sparser

"In addition, we detected the effect of USP33-integrin α6 regulation on cell metastasis in vivo."

39589547

➶

sparser

"USP33 binds and stabilizes integrin α6 through deubiquitination."

39589547

➶

sparser

"The result showed that USP33 could interact with integrin α6 and β4 (Fig. xref B)."

39589547

➶

sparser

"Altogether, these results indicate that USP33 could bind and stabilize integrin α6 through deubiquitination."

39589547

➶

sparser

"As described above, USP33 could bind and deubiquitinate integrin α6 to stabilize its expression; it is reasonable to infer that the USP33-integrin α6 axis plays an essential role in ESCC cell migration on laminin."

39589547

➶

reach

"The interaction between USP33 and integrins was detected by immunoprecipitation, and the deubiquitination was performed by deubiquitination assay."

39589547

➶

reach

"USP33 binds and stabilizes integrin alpha6 through deubiquitination."

39589547

➶

sparser

"The interaction between USP33 and integrins was detected by immunoprecipitation, and the deubiquitination was performed by deubiquitination assay."

39589547

➶

sparser

"Mechanistically, USP33 can interact with integrin α6 and subsequently promote its deubiquitination and stabilization."

39589547

➶

sparser

39589547

USP33 affects DUSP1

2 1 | 4 2 1

USP33 binds DUSP1.

2 | 1 2 1

USP33 binds DUSP1. 2 / 4

2 | 1 1

➶

sparser

"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

➶

reach

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

USP33 binds DUSP1. 1 / 1

| 1

➶

isi

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

reach

"Therefore, USP33 overexpression in prostate cancer may contribute to docetaxel resistance by inhibiting the degradation of its partner DUSP1, leading to impaired JNK activation and apoptosis."

31857702

Integrins affects USP33

| 2 8

USP33 binds Integrins. 10 / 10

| 2 8

➶

sparser

"In addition, we detected the effect of USP33-integrin α6 regulation on cell metastasis in vivo."

39589547

➶

sparser

"USP33 binds and stabilizes integrin α6 through deubiquitination."

39589547

➶

sparser

"The result showed that USP33 could interact with integrin α6 and β4 (Fig. xref B)."

39589547

➶

sparser

"Altogether, these results indicate that USP33 could bind and stabilize integrin α6 through deubiquitination."

39589547

➶

sparser

39589547

➶

reach

"The interaction between USP33 and integrins was detected by immunoprecipitation, and the deubiquitination was performed by deubiquitination assay."

39589547

➶

reach

"USP33 binds and stabilizes integrin alpha6 through deubiquitination."

39589547

➶

sparser

"The interaction between USP33 and integrins was detected by immunoprecipitation, and the deubiquitination was performed by deubiquitination assay."

39589547

➶

sparser

"Mechanistically, USP33 can interact with integrin α6 and subsequently promote its deubiquitination and stabilization."

39589547

➶

sparser

39589547

USP33 affects ARRB

| 9

USP33 binds ARRB. 8 / 8

| 8

➶

sparser

"Thus, it could be speculated that AKT exerts two distinct effects on the homologous desensitization of GPCRs: one mechanism involves AKT regulating receptor desensitization by promoting the nuclear entry of Mdm2, while an alternative mechanism involves the promotion of the interaction of USP33 with β-arrestin."

38566235

➶

sparser

"The detailed steps to assay the kinetics of β-arrestin–USP33 interaction are described later."

24377933

➶

sparser

"Thus, USP33-beta-arrestin interaction is a key regulatory step in 7TMR trafficking and signal transmission from the activated receptors to downstream effectors."

19363159

➶

sparser

"Conversely, stimulation of the vasopressin V2R, a Class B receptor known to have prolonged interaction with β-arrestins, does not enhance β-arrestin 2-USP33 interaction."

21476968

➶

sparser

"These DUBs are constitutively bound to the cell-surface β 2 ARs; however, β 2 AR–USP association decreases upon agonist activation, while simultaneously agonist stimulation leads to the recruitment of[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23764054

➶

sparser

"Specific conformations of β-arrestins induced by different 7TMR binding can also determine the kinetics and stability of interaction of β-arrestin with the deubiquitinating enzyme USP33 [ xref ]."

21680031

➶

sparser

"Further, the association of the deubiquitinase USP33 with β-arrestin 2 was shown to regulate 7TMR-β-arrestin 2 interaction with greater USP33-β-arrestin 2 association resulting in decreased 7TMR-β-arrestin 2 interaction and loss of localized intracellular signaling [ xref ]."

21476968

➶

sparser

"The association of USP33 and β-arrestin 2 itself appears to be dependent on the class of 7TMR as activation of the β2-adrenergic receptor (β2AR), a Class A receptor known to have a transient interaction with β-arrestins, enhances the association of β-arrestin 2 with USP33 [ xref ]."

21476968

USP33 binds ARRB and 7TMR. 1 / 1

| 1

➶

sparser

21476968

USP33 affects SELENBP1

2 | 2 2

SELENBP1 binds USP33. 4 / 8

2 | 2 2

➶

reach

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells (Jeong et al., 2009), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

➶

sparser

"SELENBP1 has also been implicated in ubiquitination from a study demonstrating the binding of SELENBP1 to von Hippel- Lindau protein (pVHL)- interacting deubiquitinating enzyme 1 (VDU1) that was abolished with the incubation of β-mercaptoethanol, which dissociates selenium (Jeong et al., xref ), suggesting a selenium dependent interaction of SELENBP1 and VDU1."

23805071

➶

reach

"SELENBP1 has also been implicated in ubiquitination from a study demonstrating the binding of SELENBP1 to von Hippel- Lindau protein (pVHL) - interacting deubiquitinating enzyme 1 (VDU1) that was abolished with the incubation of beta-mercaptoethanol, which dissociates selenium, suggesting a selenium dependent interaction of SELENBP1 and VDU1."

23805071

➶

sparser

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells ( xref ), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

USP33 affects IRF9

| 2 6

USP33 binds IRF9.

| 6

IRF9 binds USP33. 6 / 6

| 6

➶

sparser

"Collectively, SARS-CoV-2 not only regulates the levels of circulating miRNAs activating cytokines, but also mediates the alternative pathways of CNS injury by inhibiting the USP33-IRF9 axis."

36593971

➶

sparser

"Therefore, the hyperactivation of the USP33-IRF9 axis regulated by exosomal miRNA in human microglia uncovers a bystander pathway of SARS-CoV-2-mediated CNS damage."

36977948

➶

sparser

"They also deregulated the USP33-IRF9 network following their internalization in human microglia cells."

36077138

➶

sparser

"Disruption of USP33-IRF9 axis stimulate the non-canonical activation of pro-inflammatory genes from microglia and lead to severe neuroinflammation inside CNS ( xref )."

33936086

➶

sparser

"Disruption of the USP33-IRF9 axis stimulates the noncanonical activation of pro-inflammatory genes from microglia and leads to severe neuroinflammation in the central nervous system (CNS)."

2 | 2 2

➶

reach

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells (Jeong et al., 2009), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

➶

sparser

23805071

➶

reach

"SELENBP1 has also been implicated in ubiquitination from a study demonstrating the binding of SELENBP1 to von Hippel- Lindau protein (pVHL) - interacting deubiquitinating enzyme 1 (VDU1) that was abolished with the incubation of beta-mercaptoethanol, which dissociates selenium, suggesting a selenium dependent interaction of SELENBP1 and VDU1."

23805071

➶

sparser

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells ( xref ), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

USP33 is modified

| 8

USP33 is ubiquitinated.

| 5

USP33 is ubiquitinated. 5 / 5

| 5

➶

sparser

"Unexpectedly, we found that overexpression of β-TrCP can also increase the ubiquitination of FH-USP33(△DSG) ( Fig. 2 D ), and knockdown of β-TrCP also decreased the ubiquitin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

➶

sparser

"Here, our study for the first time uncovered that β-TrCP plays the roles in tumor cell proliferation and invasion by promoting ubiquitination and degradation of USP33."

28506875

➶

sparser

"USP33 was found to be ubiquitinated in a VHL-dependent manner which targeted it for proteasomal degradation, and VHL mutations that disrupt the protein’s interaction with the DUB abrogated USP33 ubiquitination."

18687060

➶

sparser

"Data from the literature indicate that pVHL30 mediates the ubiquitination and degradation of both USP33 and USP20, in turn down-regulating the pathways controlled by these two proteins [ xref ]."

35205757

➶

sparser

11739384

USP33 is phosphorylated.

| 3

USP33 is phosphorylated. 3 / 3

| 3

➶

sparser

"The phosphorylated USP33 deubiquitinated arrestins locally, rather than globally, because USP33 specifically bound to arrestins associated with D 3 R and Mdm2 (Fig. xref )."

36658650

➶

sparser

"A recent study revealed that AKT regulates the desensitization of D 2 -like receptors by phosphorylating the deubiquitinase USP33 [ xref ]."

38566235

➶

sparser

"The phosphorylated Akt subsequently phosphorylated USP33, which was recruited to the D 3 R under desensitization conditions (Figs. xref and xref )."

36658650

AKT affects USP33

| 3 5

AKT binds USP33.

| 1 4

AKT binds USP33. 5 / 5

| 1 4

➶

reach

"We observed that whereas the interaction between Akt and USP33 increased under the desensitization conditions (Fig. 6A), this response was absent when we used USP33 harboring a mutated T154 residue, which is a known consensus Akt phosphorylation site (Fig. 6B)."

36658650

➶

sparser

"The desensitization-induced increase in the interaction between Akt and USP33 (Fig. xref A), and the nuclear translocation of Gβγ and arrestin (Fig. xref B) were also inhibited by pretreatment with AG1478."

39468452

➶

sparser

"Indeed, biochemical changes in cellular components constituting the GPCR desensitization cascade were evoked when cells were treated with EGF, such as phosphorylation of Src (Fig. xref A), Akt (Fig. xref B), the interaction between Akt and USP33 (Fig. xref C), which culminated in the deubiquitination of arrestins (Fig. xref D)."

39468452

➶

| 1 3

➶

sparser

"Mechanistically, USP33 interacted with TRAF3 and induced TRAF3 deubiquitination to up-regulate its expression (P < 0.05)."

39637362

➶

sparser

"Moreover, USP33-TRAF3 activated the NF-κB pathway (P < 0.05)."

39637362

➶

sparser

"Protein interaction between USP33 and TRAF3 was identified by immunofluorescence staining."

39637362

➶

reach

"Protein interaction between USP33 and TRAF3 was identified by immunofluorescence staining.Results: Caerulein dose-dependently induced apoptosis, oxidative stress, and inflammatory response in HPDE6-C7 cells and promoted macrophage M1 polarization to enhance inflammation (P < 0.05)."

39637362

USP33 deubiquitinates TRAF3.

| 3

USP33 deubiquitinates TRAF3. 3 / 3

| 3

➶

reach

"USP33 promotes caerulein-induced apoptotic, oxidative and inflammatory injuries in acute pancreatitis by deubiquitinating TRAF3."

39637362

➶

reach

"Conclusion: USP33 promoted caerulein-induced apoptosis, oxidative stress and inflammation in pancreatic ductal cells by deubiquitinating TRAF3, indicating a novel insight into the pathogenesis of AP."

39637362

➶

reach

"Mechanistically, USP33 interacted with TRAF3 and induced TRAF3 deubiquitination to up-regulate its expression (P < 0.05)."

39637362

USP33 affects JAK2

1 | 4 2

USP33 binds JAK2.

1 | 1 2

USP33 binds JAK2. 3 / 4

1 | 1 2

➶

sparser

"In the present experiment, we found for the first time that USP33 could directly interact with JAK2 and maintain its protein stability by reducing its ubiquitination modification, thus activating JAK2/STAT3 signaling pathway."

37256445

➶

sparser

"Then, we further determined the interaction between USP33 and JAK2."

37256445

➶

reach

"Then, we further determined the interaction between USP33 and JAK2."

37256445

USP33 deubiquitinates JAK2.

| 3

USP33 deubiquitinates JAK2. 3 / 3

| 3

➶

reach

"From the UbiBrowser _V2 data, we found that JAK2 was a potential target for USP33, suggesting that USP33 probably participated in the deubiquitination of JAK2 protein (Fig. 5A)."

37256445

➶

reach

"Further in vivo ubiquitination assay verified that USP33 overexpression significantly decreased JAK2 ubiquitination compared with the control group (Fig. 5C)."

37256445

➶

reach

"All those results demonstrated that USP33 overexpression activated JAK2/STAT3 signaling pathway through deubiquitinating JAK2."

37256445

USP33 affects GCGR

1 | 5 1

USP33 deubiquitinates GCGR.

| 3

USP33 deubiquitinates GCGR. 3 / 3

| 3

➶

reach

"USP33 knockdown drastically increases GCGR ubiquitination in quiescent cells; one possibility is that USP33 deubiquitinates and protects newly synthesized receptors from 26S proteasomal degradation."

32967969

➶

| 3

USP33 inhibits CXCR4. 3 / 5

| 3

➶

reach

"We demonstrated that USP33 overexpression can attenuate CXCR4 internalization, while USP33 knock-down showed the opposite effect."

27835898

➶

reach

"On the other hand, we tested the level of total receptors after SDF-1 stimulation and demonstrated that USP33 knock-down can increase the degradation of CXCR4, while USP33 overexpression can inhibit its degradation."

27835898

➶

reach

"We also demonstrated that in the LoVo cells transfected with CXCR4, overexpression of beta-arrestin2 can further promote cell proliferation, while co-overexpression of USP33 can attenuate the oncogenetic effects of CXCR4 (XREF_SUPPLEMENTARY)."

27835898

USP33 activates CXCR4.

| 2

USP33 activates CXCR4. 2 / 2

| 2

➶

reach

"and silencing USP33 can promote internalization and degradation of CXCR4."

27835898

➶

reach

"We have proved that USP33 knock-down can promote the internalization and degradation of CXCR4."

27835898

USP33 affects CTNNB1

3 | 3 1

USP33 binds CTNNB1.

3 | 1 1

USP33 binds CTNNB1. 2 / 5

3 | 1 1

➶

sparser

"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

| 6

➶

reach

"More importantly, DUSP1 overexpression could reverse the USP33 knockdown induced JNK activation and apoptosis in docetaxel treated prostate cancer cells."

31857702

➶

reach

"Previous studies showed that USP33 promotes cell proliferation and reduces apoptosis in retinoblastoma [ 36 ]."

37267862

➶

reach

"Additionally, accumulated evidence has demonstrated that USP33 was elevated in prostates cancer and USP33 knockdown increased apoptosis of prostates cancer cells [ 37 ]."

37267862

➶

reach

"Ubiquitin specific peptidase 33 promotes cell proliferation and reduces apoptosis through regulation of the SP1/PI3K/AKT pathway in retinoblastoma."

34470581

➶

reach

"In retinoblastoma, USP33 was reported to regulate cell proliferation and inhibit apoptosis via stabilizing SP1 and activation of SP1/PI3K/Akt signaling pathway [47]."

36582601

➶

reach

"USP33 overexpression impedes paclitaxel-triggered apoptosis in resistant prostate cancer cells."

38702734

USP33 affects Slit-Robo

| 6

USP33 activates Slit-Robo. 6 / 6

| 6

➶

reach

"Recently, Huang et al. found that the USP33 mediates Slit-Robo signaling in inhibiting CRC cell migration [XREF_BIBR]."

26674478

➶

reach

"USP33 mediates Slit-Robo signaling in lung cancer cell migration."

24981056

➶

reach

"USP33, a new player in lung cancer, mediates Slit-Robo signaling."

24981056

➶

reach

"USP33 mediates Slit-Robo signaling in inhibiting colorectal cancer cell migration."

25242263

➶

reach

"USP33 can also inhibit tumor progression of colorectal cancer by targeting Slit-Robo signaling pathway or beta-arrestin signaling [XREF_BIBR, XREF_BIBR]."

29574466

➶

reach

"USP33 can regulate centrosome amplication [XREF_BIBR], recycling of beta-adrenergic receptor [XREF_BIBR, XREF_BIBR], and mediate Slit-Robo signaling in carcinoma [XREF_BIBR, XREF_BIBR]."

27835898

USP33 affects FGFR2

| 6

USP33 binds FGFR2. 6 / 6

| 6

➶

sparser

"Before starting pemigatinib, her plasma cfDNA FGFR2-USP33 fusion was detected at 1.1% VAF and subsequently decreased to an undetectable level two weeks after starting treatment (Fig. xref )."

39289482

➶

sparser

"In the FIGHT-101 trial, a patient with KRAS WT PDAC harboring an FGFR2-USP33 fusion achieved a partial response to pemigatinib that was maintained for 10.7 months before disease progression [ xref ]."

36551707

➶

sparser

"In the FIGHT-101 clinical trial, one of five patients with pancreatic cancer showed a partial response to anti-FGFR1-3 treatment (pemigatinib); this case was positive for FGFR2::USP33 fusion [ xref ]."

38542259

➶

sparser

"C382R mutation, and FGF3,4,19 amplifications), and 1 each with gallbladder cancer ( FGFR2-BICC1 fusion), pancreatic cancer ( FGFR2-USP33 fusion), urothelial cancer ( FGFR3 p."

35176457

➶

sparser

"Following disease progression on this trial, genomic testing revealed an FGFR2-USP33 fusion, and she subsequently started a basket trial for the FGFR-selective inhibitor pemigatinib where she exhibited a partial response with a 43% reduction in tumor size (NCT02393248) and remained on therapy for 28 months (Fig. xref , Supplementary Figs. xref , xref , xref ) xref ."

39289482

➶

sparser

"One in four patients with pancreatic cancer had FGFR2-USP33 fusion and maintained a response for 10.7 months."

37893023

USP33 affects ARR3

1 | 5

ARR3 binds USP33. 5 / 6

1 | 5

➶

reach

"Akt facilitates the interaction between USP33 and arrestin3."

36658650

➶

reach

"Immunocytochemical analysis showed that USP33 formed a complex with arrestin3 under desensitization conditions (Fig. 3D; Quin, w +)."

36658650

➶

reach

"As shown in Fig. 3A and B, interactions between USP33, D R, and arrestin3 remained similar under non-desensitization conditions (30 nM DA, w +), but increased under desensitization conditions (100 nM Quin, w +)."

36658650

➶

reach

"In addition, an interaction between USP33 and arrestin3 occurred in an Mdm2-dependent manner (Fig. 3C)."

36658650

➶

reach

"Consistent with the inhibition of arrestin3 deubiquitination by pretreatment with triciribine (Fig. 5B), this pretreatment was found to block the interaction between arrestin3 and USP33 (Fig. 6E)."

36658650

IRF9 affects USP33

| 6

IRF9 binds USP33. 6 / 6

| 6

➶

sparser

"Collectively, SARS-CoV-2 not only regulates the levels of circulating miRNAs activating cytokines, but also mediates the alternative pathways of CNS injury by inhibiting the USP33-IRF9 axis."

36593971

➶

sparser

"Therefore, the hyperactivation of the USP33-IRF9 axis regulated by exosomal miRNA in human microglia uncovers a bystander pathway of SARS-CoV-2-mediated CNS damage."

36977948

➶

sparser

"They also deregulated the USP33-IRF9 network following their internalization in human microglia cells."

36077138

➶

sparser

"Disruption of USP33-IRF9 axis stimulate the non-canonical activation of pro-inflammatory genes from microglia and lead to severe neuroinflammation inside CNS ( xref )."

33936086

➶

sparser

"Disruption of the USP33-IRF9 axis stimulates the noncanonical activation of pro-inflammatory genes from microglia and leads to severe neuroinflammation in the central nervous system (CNS)."

36593971

➶

sparser

"Mishra, R. and Banerjea, A. C., SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia."

| DOI

FGFR2 affects USP33

| 6

USP33 binds FGFR2. 6 / 6

| 6

➶

sparser

"Before starting pemigatinib, her plasma cfDNA FGFR2-USP33 fusion was detected at 1.1% VAF and subsequently decreased to an undetectable level two weeks after starting treatment (Fig. xref )."

39289482

➶

sparser

36551707

➶

sparser

38542259

➶

sparser

"C382R mutation, and FGF3,4,19 amplifications), and 1 each with gallbladder cancer ( FGFR2-BICC1 fusion), pancreatic cancer ( FGFR2-USP33 fusion), urothelial cancer ( FGFR3 p."

35176457

➶

sparser

39289482

➶

sparser

"One in four patients with pancreatic cancer had FGFR2-USP33 fusion and maintained a response for 10.7 months."

37893023

DUSP1 affects USP33

2 | 1 2 1

USP33 binds DUSP1. 2 / 4

2 | 1 1

➶

sparser

34666042

➶

reach

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

USP33 binds DUSP1. 1 / 1

| 1

➶

isi

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

JNK binds USP33 and DUSP1. 1 / 1

| 1

➶

sparser

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

ARR3 affects USP33

1 | 5

ARR3 binds USP33. 5 / 6

1 | 5

➶

reach

"Akt facilitates the interaction between USP33 and arrestin3."

36658650

➶

reach

"Immunocytochemical analysis showed that USP33 formed a complex with arrestin3 under desensitization conditions (Fig. 3D; Quin, w +)."

36658650

➶

reach

36658650

➶

reach

"In addition, an interaction between USP33 and arrestin3 occurred in an Mdm2-dependent manner (Fig. 3C)."

36658650

➶

reach

"Consistent with the inhibition of arrestin3 deubiquitination by pretreatment with triciribine (Fig. 5B), this pretreatment was found to block the interaction between arrestin3 and USP33 (Fig. 6E)."

36658650

Lipopolysaccharide affects USP33

| 5

Lipopolysaccharide activates USP33. 5 / 5

| 5

➶

reach

"Further RT-qPCR experiments showed that LPS stimulation induced significant downregulation of miR-206 (Fig. 1B) and remarkable upregulation of USP33 (Fig. 1C)."

37256445

➶

reach

"In addition, the activation of JAK2/STAT3 by coumermycin A1 remarkably reversed the protective role of miR-206 in LPS-induced inflammatory injuries, suggesting JAK2/STAT3 signaling was a key downstream pathway of miR-206/USP33 in SCM.In this study, our data revealed that miR-206 alleviated LPS-induced inflammatory injuries by directly targeting USP33 and suppressing the activation of JAK2/STAT3 signaling pathway."

37256445

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"Correction: miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway."

37432634

➶

reach

"miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway."

37256445

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reach

"Gain-of-function of miR-206 promoted the proliferation but suppressed the inflammatory responses and apoptosis of LPS-induced cardiomyocytes by targeting USP33 and suppressing the JAK2/STAT3 signaling pathway."

37256445

USP33 affects SP1

| 1 2 2

USP33 binds SP1.

| 2 2

USP33 binds SP1. 4 / 4

| 2 2

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"Intriguingly, the results indicated that endogenous USP33 and SP1 are directly bound in HCC cells ( Fig. 5 C and Supplemental Fig. 7A)."

32835698

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reach

"Mechanistically, USP33 directly bound SP1 and decreased its ubiquitination, thereby upregulating c-Met expression."

32835698

➶

reach

"Mechanistically, USP33 directly bound SP1 protein and decrease its ubiquitination and enhance its protein stability, thereby upregulating c-Met expression which eventually promoted the migration and invasion of HCC [40]."

37322017

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sparser

"To this end, we first performed Co-IP experiments to detect the interaction between USP33 and SP1 proteins in HCC cells."

32835698

USP33 deubiquitinates SP1.

| 1

➶

reach

"USP33 de-ubiquitinates mono-ubiquitinated RALB, which allows RALB to associate with Beclin1-containing complexes to positively induce autophagy [ 16 ]."

29462615

USP33 affects E7

2 1 | 1 1

USP33 binds E7. 1 / 4

2 1 | 1

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"The Gaussia princeps luciferase protein complementation assay shows that USP15 interacts with the key oncoprotein human papillomavirus (HPV) E6, while USP29 and USP33 bind to E7 protein, suggesting that cellular DUBs impact HPV tumorigenesis by regulating the stability of the viral proteins [45] ."

33922750

USP29 binds USP33 and E7. 1 / 1

| 1

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33922750

USP33 affects DUSP2

| 5

DUSP2 binds USP33. 5 / 5

| 5

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34666042

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"We were successful in producing the USP4-DUSP, USP11-DUSP, USP15-DUSP, USP20-DUSP2 and USP33-DUSP2 recombinant proteins in Escherichia coli and purified them to near homogeneity from the soluble fract[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

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"Under native conditions, size-exclusion chromatography (SEC) demonstrated that each DUSP protein existed as a stable monomer, except USP33-DUSP2, which eluted as two peaks with one peak corresponding [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

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"Finally, among six UbVs selected for binding to USP33-DUSP2, three exhibited some non-specific binding to SAV (33D2.4–6) but the other three exhibited absolute specificity for their cognate target (33[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

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"For USP15-DUSP and USP33-DUSP2, the best UbVs exhibited affinities in the low nanomolar range (K D ∼ 10 nM), whereas for USP11-DUSP and USP20-DUSP2, affinities were in the mid-nanomolar range (K D ∼ 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

USP33 affects AR

| 3 2

USP33 binds AR.

| 1 2

USP33 binds AR. 2 / 2

| 1 1

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"Usp33 interacts with β 2 AR but mechanistically how this occurs is not known."

23201781

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reach

"Usp33 interacts with beta 2 AR but mechanistically how this occurs is not known."

23201781

AR binds ARRB2 and USP33. 1 / 1

| 1

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23764054

USP33 deubiquitinates AR.

| 2

USP33 leads to the deubiquitination of AR. 2 / 2

| 2

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"Ubiquitination of the beta 2 AR is reversed by the deubiquitinases USP20 and USP33, which tonically associate with the beta 2 AR."

23166351

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"104 Two DUBs USP20 and USP33 have been shown to mediate deubiquitination of beta 2 AR."

26055058

USP33 affects AKT

| 1 4

AKT binds USP33. 5 / 5

| 1 4

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36658650

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39468452

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39468452

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36658650

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36658650

HERC2 affects USP33

1 | 3 1

HERC2 binds USP33.

1 | 1 1

HERC2 binds USP33. 1 / 2

1 | 1

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"13 HERC2 also interacts with the deubiquitinating enzyme USP33 and the SCF protein FBXL5, regulating their stability through ubiquitin mediated proteasomal degradation."

2 1 | 1 1

USP33 binds E7. 1 / 4

2 1 | 1

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33922750

USP29 binds USP33 and E7. 1 / 1

| 1

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33922750

DUSP2 affects USP33

| 5

DUSP2 binds USP33. 5 / 5

| 5

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34666042

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34666042

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34666042

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34666042

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34666042

CTNNB1 affects USP33

3 | 1 1

USP33 binds CTNNB1. 2 / 5

3 | 1 1

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"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

37076992

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"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

37076992

GroEL2 affects USP20

| 4

GroEL2 binds USP33 and USP20. 4 / 4

| 4

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sparser

24874852

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sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

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sparser

24874852

ZEB1 affects USP33

| 3 1

ZEB1 increases the amount of USP33.

| 2

ZEB1 increases the amount of USP33. 2 / 2

| 2

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"Moreover, the downstream gene of TGFβ named ZEB1 was intriguing the transcriptional regulator of USP33, we found that ZEB1 promoted the transcription of USP33 through binding to the 622-632 site of USP33 promoter."

| 4

➶

reach

39589547

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reach

39589547

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reach

"USP33 promotes laminin-dependent cell adhesion and migration."

39589547

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reach

"USP33 promoted laminin-dependent adhesion, spreading, and migration of ESCC cells but not their proliferation."

39589547

USP33 affects glycolytic process

| 4

USP33 activates glycolytic process. 4 / 4

| 4

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37267862

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"Moreover, overexpression of USP33 caused marked enhancements in glycolysis, which was reversed by c -Myc inhibitor 10058-F4."

37267862

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reach

"It is suggested that USP33 promotes aerobic glycolysis in OS cells and inhibits mitochondrial respiration."

38288377

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reach

"Those results suggested that Usp33 knockdown inhibits activation and glycolysis of primary hepatic stellate cells.Usp33 overexpression promotes activation and glycolysis of primary hepatic stellate ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37267862

USP33 affects cell growth

| 4

USP33 inhibits cell growth.

| 2

USP33 inhibits cell growth. 2 / 2

| 2

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"MTT results showed that USP33 can inhibit cellular growth (XREF_FIG)."

29802710

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reach

"Unlike USP33, which has been reported to inhibit mitogen activated protein kinase (MAPK) activation pathway and suppress hepatoma cell growth 26, our study suggests that USP21 activates ERK1/2 to promote HCC cell proliferation."

29706623

USP33 activates cell growth.

| 2

USP33 activates cell growth. 2 / 2

| 2

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"Functional assays suggested that USP33 promoted OS cell growth through the induction of aerobic glycolysis."

37034227

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reach

39589547

USP33 affects TGFB

| 3 1

USP33 activates TGFB.

| 2

USP33 activates TGFB. 2 / 2

| 2

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| 2

USP33 inhibits Slit. 2 / 2

| 2

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"Downregulation of USP33 inhibits Slit and Robo signaling pathway and is associated with poor patient survival of glioma."

32972109

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reach

"Mechanistically, USP33 inhibits the migration of glioma cells by regulating the function of the Slit/Robo signaling pathway [76]."

40149733

USP33 activates Slit.

| 2

USP33 activates Slit. 2 / 2

| 2

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| 2

USP33 activates Neoplasms. 2 / 2

| 2

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reach

"USP33/TGFBR2 axis promoted the tumor growth and metastasis of PC in vivo."

37322017

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reach

"Taking together, our results demonstrated that USP33 promoted the tumor growth and metastasis of PC in a TGFBR2-dependent manner."

37322017

USP33 affects HIF1A

| 4

USP33 deubiquitinates HIF1A.

| 2

USP33 deubiquitinates HIF1A. 2 / 2

| 2

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"Mechanistically, USP33 deficiency led to an elevation in HIF1A ubiquitination and degradation."

| 2

USP33 inhibits Glioma. 2 / 2

| 2

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reach

"In vitro studies have suggested that the overexpression of USP33 can significantly inhibit the migratory ability of glioma cells."

40149733

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reach

"Mechanistically, USP33 inhibits the migration of glioma cells by regulating the function of the Slit/Robo signaling pathway [76]."

40149733

USP33 activates Glioma.

| 2

USP33 activates Glioma. 2 / 2

| 2

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"Under hypoxic conditions, deubiquitinase USP33 promotes glioblastoma growth and maintains stemness of glioma stem cells by stabilizing hypoxia inducible factor-2α [12]."

38863009

➶

2 | 2

➶

sparser

"Moreover, we predicted more precise regions and sites of the interaction between E protein and USP33 T2 domain by AlphaFold 3."

39301916

➶

sparser

"The CO‐IP assay confirmed the existence of an interaction between USP33 and the E protein (Figure xref , Supporting Information)."

39301916

USP20 affects groEL2

| 4

GroEL2 binds USP33 and USP20. 4 / 4

| 4

➶

sparser

24874852

➶

sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

➶

sparser

24874852

TRAF3 affects USP33

| 1 3

USP33 binds TRAF3. 4 / 4

| 1 3

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sparser

"Mechanistically, USP33 interacted with TRAF3 and induced TRAF3 deubiquitination to up-regulate its expression (P < 0.05)."

39637362

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sparser

"Moreover, USP33-TRAF3 activated the NF-κB pathway (P < 0.05)."

39637362

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sparser

"Protein interaction between USP33 and TRAF3 was identified by immunofluorescence staining."

39637362

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reach

39637362

SP1 affects USP33

| 2 2

USP33 binds SP1. 4 / 4

| 2 2

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sparser

"Intriguingly, the results indicated that endogenous USP33 and SP1 are directly bound in HCC cells ( Fig. 5 C and Supplemental Fig. 7A)."

32835698

➶

reach

"Mechanistically, USP33 directly bound SP1 and decreased its ubiquitination, thereby upregulating c-Met expression."

32835698

➶

reach

37322017

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sparser

"To this end, we first performed Co-IP experiments to detect the interaction between USP33 and SP1 proteins in HCC cells."

32835698

JAK2 affects USP33

1 | 1 2

USP33 binds JAK2. 3 / 4

1 | 1 2

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sparser

37256445

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"Then, we further determined the interaction between USP33 and JAK2."

37256445

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reach

"Then, we further determined the interaction between USP33 and JAK2."

37256445

GCGR affects USP33

1 | 2 1

GCGR binds USP33.

1 | 1 1

GCGR binds USP33. 2 / 3

1 | 1 1

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reach

"The USP33-GCGR complex likely dissembles at later times of agonist, as suggested by the decreasing interaction seen in the pulldown assay at 60 min of agonist stimulation (Fig. 7, C and D)."

32967969

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"The USP33-GCGR complex likely dissembles at later times of agonist, as suggested by the decreasing interaction seen in the pulldown assay at 60 min of agonist stimulation ( xref , C and D )."

32967969

GCGR activates USP33.

| 1

GCGR activates USP33. 1 / 1

| 1 1

E3_Ub_ligase ubiquitinates USP33. 2 / 2

| 1 1

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reach

"We demonstrated that hVDU1 could interact with the beta-domain of pVHL and this interaction mediated the ubiquitination and degradation of VDU1 by pVHL E3 ligase [30]."

12056827

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sparser

"We demonstrated that hVDU1 could interact with the β-domain of pVHL and this interaction mediated the ubiquitination and degradation of VDU1 by pVHL E3 ligase [30] ."

12056827

E3_Ub_ligase increases the amount of USP33.

| 1

E3_Ub_ligase increases the amount of USP33. 1 / 1

| 1

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"However, how cellular USP33 levels are regulated remains largely unexplored.Here we discovered that the E3 ligase beta-TrCP did not reduce USP33 mRNA levels, but significantly downregulated USP33 prot[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

E3_Ub_ligase decreases the amount of USP33.

| 1

E3_Ub_ligase decreases the amount of USP33. 1 / 1

| 1

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28506875

E affects USP33

2 | 2

E binds USP33. 2 / 4

2 | 2

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"Moreover, we predicted more precise regions and sites of the interaction between E protein and USP33 T2 domain by AlphaFold 3."

39301916

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sparser

"The CO‐IP assay confirmed the existence of an interaction between USP33 and the E protein (Figure xref , Supporting Information)."

39301916

USP33 affects α-SMA

| 3

USP33 increases the amount of α-SMA.

| 2

USP33 increases the amount of α-SMA. 2 / 2

| 2

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"Conversely, the expression of α-SMA, COL1A2, and Hyp content were decreased by the USP33 knockdown, suggesting the hepatic stellate cell activation was downregulated by the USP33 knockdown."

37267862

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reach

"In addition, the protein levels of α-SMA, COL1A2, LDHA, and c -Myc were significantly reduced by Usp33 knockdown in primary hepatic stellate cells ( Fig. 3 F and G)."

37267862

USP33 activates α-SMA.

| 1

USP33 activates α-SMA. 1 / 1

| 1

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reach

"Moreover, the upregulation of Usp33, α-SMA, COL1A2, and LDHA induced by Usp33 were suppressed by the 10058-F4 ( Fig. 4 F)."

37267862

USP33 affects integrin α6

| 3

USP33 binds integrin α6.

| 2

USP33 binds integrin α6. 2 / 2

| 2

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"Altogether, these results indicate that USP33 could bind and stabilize integrin α6 through deubiquitination."

39589547

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reach

39589547

USP33 activates integrin α6.

| 1

USP33 activates integrin α6. 1 / 1

| 1

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reach

39589547

USP33 affects centriole

| 3

USP33 activates centriole. 3 / 3

| 3

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eidos

"Moreover , knockdown of USP33 reverses centriole amplification in both NudCL2 KO and HERC2-depleted cells ."

31427565

➶

eidos

"Knockdown of USP33 suppressed centriole amplification in NudCL2 KO cells ( Fig. 6g-i ) ."

31427565

➶

eidos

"Moreover , downregulation of USP33 reverses centriole amplification induced by NudCL2 KO or HERC2 knockdown ( Figs ."

31427565

USP33 affects cell adhesion

| 3

USP33 activates cell adhesion. 3 / 3

| 3

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"USP33 promoted laminin-dependent adhesion, spreading, and migration of ESCC cells but not their proliferation."

39589547

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reach

"USP33 promotes laminin-dependent cell adhesion and migration."

39589547

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reach

"Consistent with the observations in these types of cancer, we found that USP33 promoted cell adhesion and migration under laminin-coating conditions, indicating candidate mediator proteins in laminin receptors are involved in USP33-mediate cell migratory ability."

39589547

USP33 affects arrestins

| 3

USP33 deubiquitinates arrestins.

| 2

USP33 deubiquitinates arrestins. 1 / 1

| 1

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"These results suggest that USP33 likely locally, rather than globally, deubiquitinates arrestins associated with D R/Mdm2.Knockdown of USP33 abolished the deubiquitination of arrestin3 (Fig. 4A), the desensitization of D R (Fig. 4B), and the nuclear entry of Gβγ and arrestin3 (Fig. 4C)."

36658650

Phosphorylated USP33 deubiquitinates arrestins. 1 / 1

| 1

➶

reach

"The phosphorylated USP33 deubiquitinated arrestins locally, rather than globally, because USP33 specifically bound to arrestins associated with D R and Mdm2 (Fig. 3)."

36658650

USP33 binds arrestins.

| 1

Phosphorylated USP33 binds arrestins. 1 / 1

| 1

➶

reach

"The phosphorylated USP33 deubiquitinated arrestins locally, rather than globally, because USP33 specifically bound to arrestins associated with D R and Mdm2 (Fig. 3)."

36658650

USP33 affects USP33

| 3

USP33 inhibits USP33.

| 2

USP33 inhibits USP33. 2 / 2

| 2

➶

reach

"The miR-148a carried within these EVs inhibits the expression level of the ubiquitin-specific peptidase 33 (USP33) protein."

38255898

➶

reach

"Furthermore, beta-TrCP also increased the ubiquitination of USP33 (^ DSG) and decreased the protein stability of USP33 (^ DSG)."

28506875

USP33 increases the amount of USP33.

| 1

USP33 increases the amount of USP33. 1 / 1

| 1

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reach

37322017

USP33 affects Slit2-Robo1

| 3

USP33 inhibits Slit2-Robo1. 3 / 3

| 3

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"Correction to "Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT"."

38487955

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reach

"Several downstream effectors, including Abl [XREF_BIBR], Dock [XREF_BIBR], Ena (32), ERK1/2 [XREF_BIBR], small Rho GTPases [XREF_BIBR], USP33 [XREF_BIBR] and Vilse [XREF_BIBR], have been reported to mediate the inhibitory effect of Slit2-Robo1 signaling on cell motility, proliferation, apoptosis and angiogenesis in different cell types."

27923383

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reach

"Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT."

38487955

USP33 affects SLIT2

| 3

USP33 inhibits SLIT2.

| 2

USP33 inhibits SLIT2. 2 / 2

| 2

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"Enhanced USP33 expression increased Slit2 inhibition of cell migration (XREF_FIG), whereas knocking down USP33 blocked the inhibitory effect of Slit on cell migration (XREF_FIG)."

25242263

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"Ectopic expression of Slit2 significantly reduced the number of lung metastasis sites, and USP33 overexpression enhanced the inhibitory effect of Slit2 on tumor metastasis (XREF_FIG, XREF_SUPPLEMENTARY)."

25242263

USP33 increases the amount of SLIT2.

| 2 1

USP33 activates MET.

| 2

USP33 activates MET. 2 / 2

| 2

➶

eidos

"We found that USP33 knockdown inhibited the invasion and metastasis in HCC cells both in vitro and in vivo , which was partly dependent on c-Met ."

32835698

➶

eidos

"Mechanistically , USP33 directly bound SP1 and decreased its ubiquitination , thereby upregulating c-Met expression ."

32835698

USP33 binds MET.

| 1

USP33 binds MET. 1 / 1

| 1

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sparser

"Unexpectedly, the direct interaction between USP33 and c-Met proteins was not observed in HCC cells by coimmunoprecipitation (Co-IP) experiments ( Fig. 4 A and Supplemental Fig. 5A)."

32835698

USP33 affects LDHA

| 3

USP33 increases the amount of LDHA.

| 2

USP33 increases the amount of LDHA. 2 / 2

| 2

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"In addition, the protein levels of α-SMA, COL1A2, LDHA, and c -Myc were significantly reduced by Usp33 knockdown in primary hepatic stellate cells ( Fig. 3 F and G)."

37267862

USP33 affects FLVCR1

| 3

USP33 activates FLVCR1. 3 / 3

| 3

➶

reach

"USP33 promotes the development of PCa through two pathways."

36811009

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reach

"Thus, USP33 promotes PCa cell proliferation and self-renewal by inhibiting the degradation of CTNNB1."

37076992

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reach

"Herein, we report that USP33 silencing inhibits PCa cell survival and self-renewal."

37076992

USP33 affects Cell Survival

| 1 2

USP33 activates Cell Survival.

| 1 1

USP33 activates Cell Survival. 2 / 2

| 1 1

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reach

"Herein, we report that USP33 silencing inhibits PCa cell survival and self-renewal."

37076992

➶

eidos

"USP33 enhances cell survival and stemness by deubiquitinating CTNNB1 in BXPC-3 and SW1990 cells ."

37076992

USP33 inhibits Cell Survival.

| 1

USP33 inhibits Cell Survival. 1 / 1

sparser

"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

25242263

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

| 1

➶

sparser

"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

PFKFB3 affects USP33

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USP33 binds PFKFB3. 1 / 3

2 | 1

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37034227

Hypoxia affects USP33

| 1 2

Hypoxia activates USP33. 3 / 3

| 1 2

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"ELAVL1 decrease repressed the proliferation and microtubule formation of hypoxia-induced HRECs by reducing USP33."

39320536

➶

eidos

"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha , leading to its stabilization through deubiquitination ."

35191554

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reach

"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha, leading to its stabilization through deubiquitination."

35191554

EPAS1 affects USP33

2 | 1

EPAS1 binds USP33. 1 / 3

2 | 1

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"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha, leading to its stabilization through deubiquitination."

35191554

CRC affects USP33

| 3

CRC binds ROBO1 and USP33. 2 / 2

| 2

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sparser

"In CRC cells, USP33 interacts with Robo1 and mediates the inhibitory activity of Slit2 on CRC cell migration by deubiquitinating and stabilizing Robo1."

25242263

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sparser

"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

25242263

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

| 1

➶

sparser

"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

AR affects USP33

| 1 2

USP33 binds AR. 2 / 2

| 1 1

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sparser

"Usp33 interacts with β 2 AR but mechanistically how this occurs is not known."

23201781

➶

reach

"Usp33 interacts with beta 2 AR but mechanistically how this occurs is not known."

23201781

AR binds ARRB2 and USP33. 1 / 1

| 1

➶

sparser

23764054

7b affects USP33

2 | 1

7b binds USP33. 1 / 3

2 | 1

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sparser

"As verified by immunoprecipitation, M, E, and ORF7b significantly interacted with USP33, with the strongest binding occurring in E proteins (Figure xref )."

39301916

MiR-206 affects USP33

| 2

MiR-206 binds USP33. 2 / 2

| 2

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"To study whether miR-206 interacts with USP33, 22RV1 cells were transfected with reporter plasmid pGL3-USP33 WT, which contains the predicted binding site of miR-206, or pGL3 USP33 mutant, which contains mutations in the predicted binding site (XREF_FIG)."

33718387

➶

reach

"RIP analysis followed by qRT-PCR assay demonstrated that USP33 mRNA and miR-206 were immunoprecipitated by Ago2 (XREF_FIG), which confirmed the interaction between miR-206 and USP33."

33718387

Integrin α6 affects USP33

| 2

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reach

"USP33 can also inhibit tumor progression of colorectal cancer by targeting Slit-Robo signaling pathway or beta-arrestin signaling [XREF_BIBR, XREF_BIBR]."

29574466

| 1

USP33 inhibits Ubiquitin. 1 / 1

| 1

➶

reach

"Ubiquitin-specific peptidases (USPs), such as USP15, USP 30, and USP33, negatively regulate macromitophagy by hydrolyzing the Ub chains of mitochondrial surface proteins [13–15]."

38374038

USP33 binds Ubiquitin.

| 1

USP33 binds Ubiquitin. 1 / 1

| 1

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23486064

USP33 affects ISG75

| 2

USP33 activates ISG75. 2 / 2

| 2

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reach

"Two T. brucei orthologues of human deubiquitinases Usp7 and Vdu1 designated TbUsp7 and TbVdu1 were confirmed to be expressed in the parasite, and their knockdown increased the turnover of ISG75 and ISG65 (Zoltner et al., 2015)."

37088149

➶

| 2

➶

sparser

"In marked contrast, HA-VDU1 interacted strongly with hSP56/Se ( Fig. 2 C)."

19118533

➶

sparser

"No significant interaction between HA-VDU1 and BSA or hSP56 was observed."

19118533

USP33 affects Glioblastoma

| 2

USP33 activates Glioblastoma. 2 / 2

| 2

➶

reach

"Under hypoxic conditions, deubiquitinase USP33 promotes glioblastoma growth and maintains stemness of glioma stem cells by stabilizing hypoxia inducible factor-2α [12]."

38863009

USP20 affects NEURL4

| 2

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

➶

sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

| 2

➶

sparser

"In marked contrast, HA-VDU1 interacted strongly with hSP56/Se ( Fig. 2 C)."

19118533

➶

sparser

"No significant interaction between HA-VDU1 and BSA or hSP56 was observed."

19118533

CRC affects ROBO1, and USP33

| 2

CRC binds ROBO1 and USP33. 2 / 2

| 2

reach

38874156

USP33 affects USP8

| 1

USP33 inhibits USP8. 1 / 1

| 1

| 1

➶

reach

38874156

USP33 affects SRGAP1

| 1

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

| 1

➶

sparser

"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

USP33 affects SMAD2_3

| 1

USP33 leads to the phosphorylation of SMAD2_3. 1 / 1

| 1

➶

reach

37322017

USP33 affects SMAD

| 1

| 1

USP33 activates DNA Damage. 1 / 1

| 1

➶

reach

"Here, we demonstrate that USP33 modulates DNA damage responses including cell cycle arrest and apoptosis induction through associating with p53."

39694539

USP33 affects Cyclin

| 1

USP33 inhibits Cyclin. 1 / 1

| 1

➶

reach

"In this study, USP33 knockdown was achieved as a proof-of-concept because USP33 can antagonize cyclin F actions on CP110 in regulating centrosome duplication, maturation and separation."

26304236

USP33 affects CNTLN

| 1

| 1

➶

reach

37267862

USP33 affects ARs

| 1

ARs binds USP33 and USP20. 1 / 1

| 1

| 1

➶

sparser

"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

12056827

USP20 affects ARs

| 1

ARs binds USP33 and USP20. 1 / 1

| 1

➶

sparser

"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."

23764054

USP1 affects USP33

| 1

USP1 binds USP33. 1 / 1

| 1

➶

sparser

33146523

USP inhibitor affects USP33

| 1

USP inhibitor inhibits USP33. 1 / 1

| 1

| 1

| 1

➶

reach

"Further RT-qPCR experiments showed that LPS stimulation induced significant downregulation of miR-206 (Fig. 1B) and remarkable upregulation of USP33 (Fig. 1C)."

37256445

ROBO1 affects SRGAP1

| 1

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

| 1

➶

sparser

"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

ROBO1 affects HTATIP2

| 1

| 1

➶

sparser

"Flag‐USP33, Flag‐RNF5, Flag‐USP32, and Flag‐USP39 were purchased from SinoBiological, and USP33 was then cloned into pCAGGS or pLVX with different tags."

39301916

FGFR1 affects USP33

| 1

USP33 binds FGFR1. 1 / 1

| 1

➶

sparser

"Novel fusions were identified in 6/76 (7.9%) FN1::FGFR1 fusion-negative PMTs including two involving FGFR: FGFR1::USP33 (1/76, 1.3%), and FGFR1::TLN1 (1/76, 1.3%)."

37391169

FBXL5 affects USP33

| 1

HERC2 binds USP33 and FBXL5. 1 / 1

| 1

➶

sparser

25476789

ERAP1 affects USP33

| 1

ERAP1 activates USP33. 1 / 1

| 1

➶

reach

"In this case, ERAP1 promoted the dissociation of USP33, but not USP20, from the β2 adrenoreceptor, resulting in polyubiquitination of the receptor."

36834633

E7 affects USP29, and USP33

| 1

USP29 binds USP33 and E7. 1 / 1

| 1

➶

sparser

33922750

DUSP1 affects JNK, and USP33

| 1

JNK binds USP33 and DUSP1. 1 / 1

| 1

➶

sparser

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

DUSP1 domain affects USP33

| 1

➶

reach

"The reporter gene was activated only when AD-VDU1 f was co-transformed with BD-hSP56, supporting the specificity of the hSP56 and VDU1 f interaction.We then expressed and purified VDU1 f from E. coli [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19118533

7TMR affects USP33

| 1

USP33 binds ARRB and 7TMR. 1 / 1

| 1

➶

sparser

21476968

(R)-noradrenaline affects USP33

| 1

(R)-noradrenaline activates USP33. 1 / 1

| 1

➶

reach

"VDU1, but not VDU2, is markedly increased in brown adipocytes by norepinephrine or cold exposure, further amplifying the increase in D2 activity that results from catecholamine stimulated de novo synthesis."

12865408

IndraLab

Statements

USP33 affects ROBO1

USP33 binds ROBO1.

ROBO1 binds USP33. 10 / 34

CRC binds ROBO1 and USP33. 2 / 2

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

ROBO1 binds HTATIP2 and USP33. 1 / 1

USP33 deubiquitinates ROBO1.

USP33 deubiquitinates ROBO1. 8 / 9

Modified USP33 leads to the deubiquitination of ROBO1. 1 / 1

USP33 increases the amount of ROBO1.

USP33 increases the amount of ROBO1. 2 / 2

Modified USP33 increases the amount of ubiquitinated ROBO1. 1 / 1

Modified USP33 increases the amount of ROBO1. 1 / 1

USP33 inhibits ROBO1.

USP33 inhibits ROBO1. 2 / 3

USP33 decreases the amount of ROBO1.

USP33 decreases the amount of ubiquitinated ROBO1. 2 / 2

USP33 decreases the amount of ROBO1. 1 / 1

USP33 activates ROBO1.

USP33 activates ROBO1. 1 / 2

USP33 ubiquitinates ROBO1.

USP33 leads to the ubiquitination of ROBO1. 1 / 1

USP33 affects ARRB2

USP33 binds ARRB2.

ARRB2 binds USP33. 10 / 23

AR binds ARRB2 and USP33. 1 / 1

USP33 deubiquitinates ARRB2.

USP33 deubiquitinates ARRB2. 6 / 7

USP33 ubiquitinates ARRB2.

USP33 leads to the ubiquitination of ARRB2. 2 / 2

USP33 decreases the amount of ARRB2.

USP33 decreases the amount of ARRB2. 2 / 2

USP33 increases the amount of ARRB2.

USP33 increases the amount of ARRB2. 1 / 1

USP33 affects CCP110

USP33 binds CCP110.

USP33 binds CCP110. 6 / 13

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

USP33 activates CCP110.

USP33 activates CCP110. 4 / 8

USP33 deubiquitinates CCP110.

USP33 deubiquitinates CCP110. 4 / 5

USP33 inhibits CCP110.

USP33 inhibits CCP110. 1 / 3

USP33 ubiquitinates CCP110.

USP33 ubiquitinates CCP110. 1 / 1

USP33 affects cell population proliferation

USP33 activates cell population proliferation.

USP33 activates cell population proliferation. 10 / 18

USP33 inhibits cell population proliferation.

USP33 inhibits cell population proliferation. 10 / 12

BTRC affects USP33

BTRC binds USP33.

BTRC binds USP33. 10 / 13

BTRC activates USP33.

BTRC activates USP33. 8 / 8

BTRC ubiquitinates USP33.

BTRC leads to the ubiquitination of USP33. 2 / 2

Modified BTRC leads to the ubiquitination of USP33. 1 / 1

BTRC decreases the amount of USP33.

BTRC decreases the amount of USP33. 2 / 3

BTRC inhibits USP33.

BTRC inhibits USP33. 2 / 2

USP33 affects Neoplasm Invasiveness

USP33 activates Neoplasm Invasiveness.

USP33 activates Neoplasm Invasiveness. 10 / 19

USP33 inhibits Neoplasm Invasiveness.

USP33 inhibits Neoplasm Invasiveness. 9 / 9

ARRB2 affects USP33

ARRB2 binds USP33. 10 / 23

AR binds ARRB2 and USP33. 1 / 1

VHL affects USP33

VHL binds USP33.

VHL binds USP33. 10 / 19

VHL binds USP33 and USP20. 1 / 1

VHL ubiquitinates USP33.

VHL ubiquitinates USP33. 1 / 2

VHL activates USP33.