IndraLab

Statements

ROBO1 binds USP33. 10 / 34

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"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

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"Robo1-USP33 interaction was confirmed in transfected cells and in embryonic neurons."

19684588

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"Slit treatment did not affect Robo1-USP33 interaction."

19684588

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"To test the specificity of Robo1-USP33 interaction, we examined whether Robo1 was co-immunoprecipitated with USP20, a homolog sharing similar domain organization and 59% amino acid identity with USP33 xref ."

19684588

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"We confirmed that the endogenously expressed Robo1 and USP33 interacted with each other in the mouse brain ( xref )."

19684588

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"In summary, our experiments demonstrate that USP33 interacts with the intracellular domain of Robo1 and that USP33 is required for Slit responsiveness of commissural neurons."

19684588

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"USP33 is associated with the axonal guidance receptor Roundabout (Robo) 1 ( xref ), which plays a major role in controlling axon crossing across the midline between brain hemispheres and neuronal dendrites ( xref )."

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"USP33 interacts with Robo1 and mediates Slit2 signaling in CRC cells."

25242263

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"Our previous studies have shown that USP33 interacts with Robo1 in neurons and breast cancer cells xref , xref ."

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"These results show that Robo1 interacts with USP33 in CRC cells."

25242263

CRC binds ROBO1 and USP33. 2 / 2

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"In CRC cells, USP33 interacts with Robo1 and mediates the inhibitory activity of Slit2 on CRC cell migration by deubiquitinating and stabilizing Robo1."

25242263

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"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

25242263

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

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"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

ROBO1 binds HTATIP2 and USP33. 1 / 1

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"Co-immunoprecipitation using deletion mutants of Robo1 and USP33 showed that the Robo1 CC3 motif interacted with USP33 in the region containing the catalytic USP and substrate-recognizing DUSP domains xref ( xref )."

19684588

USP33 deubiquitinates ROBO1.

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USP33 deubiquitinates ROBO1. 9 / 9

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"Moreover, USP33 could deubiquitinate and stabilize Robo1 and was responsible for the redistribution of Robo1 to the plasma membrane [42]."

36582601

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"Robo-interacting ubiquitin-specific protease 33 (USP33) is required for the inhibitory function of Slit2 on CRC cell migration by deubiquitinating and stabilizing Robo1"

25242263

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"USP33 deubiquitinates and stabilizes Robo1."

25242263

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"These results show that USP33 mediates Slit-Robo signaling by deubiquitinating and stabilizing Robo1 in CRC cells (XREF_SUPPLEMENTARY)."

25242263

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"USP33 mediates Slit2 signaling by deubiquitinating and stabilizing Robo1."

25242263

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"Cell based assays support a model in which USP33 reverses Robo1 ubiquitination : knockdown of USP33 increases levels of ubiquitinated Robo1, while USP33 overexpression reduces ubiquitinated Robo1 levels [XREF_BIBR]."

21704388

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"Though direct deubiquitination of Robo1 by USP33 has not been reported, it is reasonable to speculate that USP33 deubiquitinates and thus rescues Robo1 from degradation during the Slit response."

21704388

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"In addition, Xia et al. demonstrated that USP33 deubiquitinates and stabilizes ROBO1, which is required for the SLIT2-mediated inhibition of EMT and GC cell metastasis [57]."

36497313

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"USP33 deubiquitinates ROBO1 in lung cancer cells to maintain its stability, thereby regulating SLIT2 activity and inhibiting cancer cell metastasis [XREF_BIBR]."

29743814

Modified USP33 leads to the deubiquitination of ROBO1. 1 / 1

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"Overexpression of wild-type USP33, but not catalytically inactive C163A mutant of USP33, decreased the ubiquitination of Robo1."

24981056

USP33 deubiquitinates ROBO1 on lysine. 1 / 1

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biopax:reactome

No evidence text available

USP33 increases the amount of ROBO1.

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USP33 increases the amount of ROBO1. 2 / 2

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"Knocking-down USP33 in H1299 cells increased the level of ubiquitinylated Robo1 in the presence of MG132, an inhibitor of the ubiquitin proteasome system."

24981056

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"USP33 silencing in A549 and SPC-A-1 cells significantly promoted the cell migration, invasion and metastasis and obviously down-regulated the expressions of SLIT2 and ROBO1."

30187867

Modified USP33 increases the amount of ubiquitinated ROBO1. 1 / 1

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"USP33 knockdown increased the ubiquitinated Robo1 level, whereas overexpression of GFP-USP33 reduced the ubiquitinated Robo1 level (XREF_SUPPLEMENTARY), suggesting that USP33 play a role in Robo1 deubiquitination."

19684588

Modified USP33 increases the amount of ROBO1. 1 / 1

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"Conversely, overexpression of wild-type USP33, not the C163A mutant USP33, in H1299 cells increased the levels of Robo1."

24981056

USP33 inhibits ROBO1.

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USP33 inhibits ROBO1. 2 / 3

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"Downregulation of USP33 reduces the protein stability of Robo1 in lung cancer cells, providing a previously unknown mechanism for USP33 function in mediating Slit activity in lung cancer cells."

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ARRB2 binds USP33. 10 / 23

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"Thus, isoproterenol stimulation induces a reciprocal pattern of USP33 interaction with the β 2 AR and β-arrestin2: dissociation of USP33 from the β 2 AR and association of USP33 with β-arrestin2."

23764054

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19363159

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No evidence text available

19363159

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No evidence text available

19363159

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"The immunoprecipitation assay showed that USP33 can interact with β-arrestin2 in an agonist-dependent manner (Figure xref )."

27835898

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No evidence text available

27835898

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24377933

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24377933

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No evidence text available

19363159

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"Upon beta 2 AR activation, the recruited beta-arrestins are rapidly ubiquitinated and deubiquitinated, and this is facilitated by a high-affinity interaction between beta-arrestin2 and USP33."

21680031

AR binds ARRB2 and USP33. 1 / 1

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23764054

USP33 deubiquitinates ARRB2.

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USP33 deubiquitinates ARRB2. 7 / 7

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"Given that the enzymatic activity of USP33 is important in regulating CXCR4 internalization, we were interested in whether USP33 can deubiquitinate beta-arrestin2 and subsequently regulate CXCR4 endocytosis."

27835898

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"Beta-arrestin-2 can be ubiquitinated by Mdm2 (see above) and deubiquitinated by the DUB enzyme USP33."

20854416

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"Importantly, a knockdown of USP20 and USP33 enhances beta-arrestin2 ubiquitination and increases beta 2 AR degradation [XREF_BIBR, XREF_BIBR]."

24680432

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ubibrowser

No evidence text available

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"Further results verified that USP33 can deubiquitinate beta-arrestin2, subsequently block the internalization of SDF-1-stimulated CXCR4, and disrupt beta-arrestin-dependent ERK activation."

27835898

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"USP33 leads to de-ubiquitination of beta-arrestin2 with non degradative effects."

27835898

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"Subsequent deubiquitination of beta-arrestin 2 by the deubiquitinase USP33 dissociates the receptor, beta, and arrestin complex."

19853559

USP33 ubiquitinates ARRB2.

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USP33 leads to the ubiquitination of ARRB2. 2 / 2

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USP33 binds CCP110. 9 / 13

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No evidence text available

23486064

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"Alternatively, excessive amounts of USP33-DM might bind CP110, blocking it from accessing SCF Cyclin F."

23486064

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"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain ( xref )."

23486064

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"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."

23486064

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23486064

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"USP33 interacts with CP110 and localizes to centrioles primarily during S and G2/M phase, the period during which centrioles duplicate and elongate."

23486064

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"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays (XREF_SUPPLEMENTARY) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 (XREF_SUPPLEMENTARY)."

23486064

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"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain (XREF_FIG)."

23486064

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No evidence text available

23486064

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

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"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

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"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."

23486064

USP33 activates CCP110.

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USP33 activates CCP110. 4 / 8

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"Further, USP33 suppression significantly reduced CP110 protein half-life from ~ 4.5 hours to ~ 2 hours (XREF_SUPPLEMENTARY)."

23486064

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"We observed that USP33 depletion did not lead to a reduction in RRM2 levels (XREF_SUPPLEMENTARY), indicating that USP33 specifically stabilizes CP110."

23486064

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"USP33 promotes the generation of excessive centriolar foci due to elevated levels of CP110 because (1) over-expression of human or murine USP33 led to CP110 up-regulation in multiple cell lines (XREF_SUPPLEMENTARY) and (2) depletion of CP110 overrode USP33 mediated generation of centriolar foci (XREF_SUPPLEMENTARY)."

23486064

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"Further, depletion of USP33 in two PDAC cell lines (Panc1 and MiaPaCa2) led to a reduction in CP110 levels (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY), indicating that USP33 stabilizes CP110 in PDAC cells."

23486064

USP33 deubiquitinates CCP110.

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USP33 deubiquitinates CCP110. 5 / 5

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"USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110"

23486064

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"For example, USP33 deubiquitylates the centrosomal protein CP110, regulating centrosome biogenesis [76] and USP33 depletion has a co-operative effect with CDK2 on the CP110-dependent clustering function mentioned earlier [77]."

31506331

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"USP33 deubiquitinates the CP110 protein, an important regulator of centrosome duplication ( Li et al., 2013 )."

25444757

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"Thus, USP33 specifically de-ubiquitylates CP110 in vitro and in vivo, explaining how USP33 stabilizes CP110."

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USP33 activates cell population proliferation. 10 / 18

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"In our research, we reported for the first time that USP33 mediated the proliferation and metastasis of pancreatic cancer through its deubiquitinating function which may provide a novel explanation for PC malignant development.USP33 was a member of the ubiquitin-specific protease (USP) family which was the largest deubiquitination enzymes (DUBs) family."

37322017

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"Function experiments revealed that USP33 overexpression promoted the proliferation, migration and invasion of PC cells while the inhibition of USP33 expression in PC cells exhibited the opposite effect."

37322017

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"In conclusion, our study found that USP33 contributed to the proliferation and metastasis of pancreatic cancer through a positive feedback loop with TGF-beta signaling pathway."

37322017

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"Next, functional rescue assays were performed to further verify that the miR-365a-3p-mediated downregulation of USP33 promotes the proliferation, migration, and invasion of lung cancer cells."

29743814

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"Functionally, our experiments demonstrate that USP33 significantly enhances ovarian cancer proliferation and metastasis in a CBX2-dependent manner."

39256572

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"Additionally, we confirmed that 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 (PFKFB3) was critical for USP33-induced proliferation and aerobic glycolysis in OS cells, and the protein expression levels of PFKFB3 and USP33 were positively correlated in the OS tissues."

37034227

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"Through our function experiments, we found that USP33 promoted the proliferation, migration and invasion of PC in vitro and in vivo."

37322017

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"Previous studies showed that USP33 promotes cell proliferation and reduces apoptosis in retinoblastoma [ 36 ]."

37267862

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"The overexpression of USP33 accelerated the proliferation, invasion and metastasis of PC in vitro and in vivo."

37322017

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"USP33 promoted the proliferation, migration and invasion of PC cells."

37322017

USP33 inhibits cell population proliferation.

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USP33 inhibits cell population proliferation. 10 / 12

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"Cellular studies with TPC-1 and BCPAP cells demonstrated that USP33 can attenuate the cell capacities of proliferation, migration and invasion."

29533940

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"Furthermore, we found that beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and cell invasion."

28506875

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"Cell experiments showed that USP33 overexpression significantly inhibited the proliferation, migration, and invasion of GAC cells."

29802710

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"USP33 inhibits cell proliferation, migration, and invasion."

27835898

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"Additionally, USP33 overexpression suppresses the proliferation, migration, and invasion of gastric adenocarcinoma cells [56]."

36497313

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"USP33 knockdown significantly upregulated tumor cell proliferation and metastasis."

29802710

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"These results indicate that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation."

28506875

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"Taken together, we found that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation and invasion.So far, the study of USP33 is still very limited."

28506875

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"Overexpression of USP33 can significantly inhibit cell proliferation, migration, and invasion."

27835898

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"Our data showed that knockdown of USP33 promoted the proliferation of both HeLa and HCT116 cells."

28506875

BTRC affects USP33

2 | 17 9

BTRC binds USP33.

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BTRC binds USP33. 10 / 13

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"We found that USP33 (^ DSG) can also interact with beta-TrCP."

28506875

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"Β-TrCP can also interact with FH-USP33(△DSG) ( Fig. 3 B ), indicating that the interaction between β-TrCP and USP33 is independent of DSG motif of USP33."

28506875

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"The interaction of β-TrCP with these USP33 mutants was evaluated."

28506875

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"Β-TrCP is able to interact with USP33, and promotes the ubiquitination and proteasomal degradation of USP33."

28506875

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"Our data showed that USP33 was able to interact with β-TrCP-WT, but unable to interact with β-TrCP-△WD40 ( Fig. 3 D )."

28506875

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"Collectively, we found that the WD40 motif of β-TrCP and 201–400 amino acid motif of USP33 are necessary for the interaction between β-TrCP and USP33."

28506875

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No evidence text available

28506875

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No evidence text available

28506875

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"We found that USP33(△DSG) can also interact with β-TrCP ( Fig. 3 B )."

28506875

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"Since β-TrCP promotes USP33 ubiquitin-dependent degradation, then we assessed the interaction between β-TrCP and USP33."

28506875

BTRC activates USP33.

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BTRC activates USP33. 8 / 8

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"Furthermore, we found that beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and cell invasion."

28506875

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"These data suggest that beta-TrCP might mediate USP33 downregulation at the protein level."

28506875

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"Taken together, these results suggest that beta-TrCP downregulates USP33 expression at the protein level but not at the mRNA level.To uncover the mechanism of USP33 downregulation mediated by beta-TrC[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

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"Our results showed that MG132 blocked beta-TrCP-mediated USP33 downregulation, suggesting that beta-TrCP promotes USP33 downregulation via the proteasome pathway."

28506875

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"The results showed that knockdown of beta-TrCP in cells slowed down the degradation rate of endogenous USP33 and therefore enhanced USP33 protein stability."

28506875

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"These results indicate that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation."

28506875

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"Taken together, we found that beta-TrCP attenuates USP33 mediated inhibition of cell proliferation and invasion.So far, the study of USP33 is still very limited."

28506875

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"Furthermore, we demonstrated that overexpression of beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and invasion."

28506875

BTRC ubiquitinates USP33.

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BTRC leads to the ubiquitination of USP33. 2 / 2

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"Furthermore, beta-TrCP also increased the ubiquitination of USP33 (^ DSG) and decreased the protein stability of USP33 (^ DSG)."

28506875

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"Furthermore, β-TrCP also increased the ubiquitination of USP33(△DSG) ( Fig. 2 D ) and decreased the protein stability of USP33(△DSG) ( Fig. 4 B )."

28506875

Modified BTRC leads to the ubiquitination of USP33. 1 / 1

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"The results showed that overexpression of beta-TrCP substantially increased the poly-ubiquitination of endogenous USP33."

28506875

BTRC decreases the amount of USP33.

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BTRC decreases the amount of USP33. 2 / 3

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28506875

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"In contrast, knockdown of beta-TrCP in HEK293T cells upregulated endogenous USP33 protein levels."

28506875

BTRC inhibits USP33.

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BTRC inhibits USP33. 2 / 2

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"The results showed that knockdown of beta-TrCP in cells slowed down the degradation rate of endogenous USP33 and therefore enhanced USP33 protein stability."

28506875

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"Thus, our data revealed a new regulatory mechanism of the deubiquitinase USP33, and uncovered an additional signaling pathway, that is, beta-TrCP downregulates USP33 to promote cell proliferation.HeLa[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

USP33 affects Neoplasm Invasiveness

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USP33 activates Neoplasm Invasiveness.

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USP33 activates Neoplasm Invasiveness. 10 / 19

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"As expected, knockdown of USP33 only attenuated KYSE-150 cell invasion (Fig. 2F)."

39589547

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"Together, our results indicate that USP33 can promote cell invasion but has no remarkable effect on cell proliferation and migration in normal cell culture conditions."

39589547

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"Since cell-matrix adhesion is closely correlated with cell migration and invasive potentials in tumors (Hamidi and Ivaska 2018; Chastney et al. 2024), we wondered whether USP33-mediated invasion is specific ECM component(s) dependent."

39589547

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eidos

"Our results reveal that USP33 acts as the deubiquitinating enzyme of SP1 and contributes to HCC invasion and metastasis through activation of the SP1 / c-Met axis ."

32835698

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"USP33 promotes cell invasion, but not growth and migration, in normal cell culture conditions."

39589547

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reach

"Next, functional rescue assays were performed to further verify that the miR-365a-3p-mediated downregulation of USP33 promotes the proliferation, migration, and invasion of lung cancer cells."

29743814

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"Through our function experiments, we found that USP33 promoted the proliferation, migration and invasion of PC in vitro and in vivo."

37322017

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"As shown in Fig. 3A, compared with other ECM components, the binding ability of USP33-overexpressed cells was significantly increased in the laminin (which is also the primary component of Matrigel) group, indicating that USP33-mediated invasion may be related to the inducement of the adhesiveness to laminin."

39589547

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"This is the first evidence for a lung cancer invasion and metastasis mechanism in which miR-365a-3p directly targets and downregulates USP33, which further promotes the proliferation, migration, and invasion activities of lung cancer."

29743814

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"Additionally, results from in vivo and in vitro studies showed that USP33 knockdown inhibits invasion, migration, and metastasis in HCC cells."

35924961

USP33 inhibits Neoplasm Invasiveness.

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USP33 inhibits Neoplasm Invasiveness. 9 / 9

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"Cellular studies with TPC-1 and BCPAP cells demonstrated that USP33 can attenuate the cell capacities of proliferation, migration and invasion."

29533940

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"Cell experiments showed that USP33 overexpression significantly inhibited the proliferation, migration, and invasion of GAC cells."

29802710

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"Overexpression of USP33 can significantly inhibit cell proliferation, migration, and invasion."

27835898

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"[USP33 suppresses lung adenocarcinoma lung cell invasion and metastasis by down-regulating SLIT2 and ROBO1 signaling pathway]."

30187867

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reach

"Furthermore, we found that beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and cell invasion."

28506875

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reach

"USP33 inhibits cell proliferation, migration, and invasion."

27835898

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reach

"Additionally, USP33 overexpression suppresses the proliferation, migration, and invasion of gastric adenocarcinoma cells [56]."

36497313

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"USP33 silencing can promote the migration, invasion and metastasis of lung adenocarcinoma cells in vitro, and the mechanism may involve IL-6 and SLIT2 and ROBO1 signaling pathways."

30187867

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"Furthermore, we demonstrated that overexpression of beta-TrCP attenuated USP33 mediated inhibition of cell proliferation and invasion."

28506875

VHL affects USP33

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VHL binds USP33.

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VHL binds USP33. 10 / 19

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"The USP33-VHL interaction may have clinical significance because naturally occurring mutations that have been found in the beta-domain of USP33 disrupt the interaction between the two proteins."

18687060

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"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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biogrid

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19706539

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19706539

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"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

12056827

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"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."

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"Finally, we demonstrate that VDU1 is able to be ubiquitinated via a pVHL dependent pathway for proteasomal degradation, and VHL mutations that disrupt the interaction between VDU1 and pVHL abrogate the ubiquitination of VDU1."

11739384

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No evidence text available

11739384

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"We propose a model, in which hSP56 may function as a novel regulator of ubiquitination-dependent protein degradation pathways by altering the enzymatic activity of VDU1 or by regulating the interactio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19118533

VHL binds USP33 and USP20. 1 / 1

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"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

12056827

ELOC binds USP33, ELOB, and VHL. 1 / 1

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hprd

No evidence text available

11739384

VHL ubiquitinates USP33.

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VHL bound to RBX1, CUL2, ELOC, and ELOB ubiquitinates USP33. 2 / 2

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signor

"VDU1 and VDU2 could be important substrates of pVHL E3 ligase complex. Finally, we demonstrate that VDU2 can also be ubiquitinated and degraded in a pVHL-dependent manner.pVHL mediates the degradation of hVDU2 by proteasome. we have demonstrated that both VDU1 and VDU2 also bind to the β-domain of pVHL and several naturally occurring mutations in the β-domain disrupt the interaction between VDU1/VDU2 and pVHL. If pVHL is mutated either in the α-domain or β-domain, VDU1 and VDU2 would not be ubiquitinated and degraded properly. This could lead to accumulation of these two proteins in the cells. Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL-related tumorigenesis."

12056827

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"Finally, we demonstrate that VDU1 is able to be ubiquitinated via a pVHL-dependent pathway for proteasomal degradation, and VHL mutations that disrupt the interaction between VDU1 and pVHL abrogate the ubiquitination of VDU1. Our findings indicate that VDU1, a novel ubiquitin-specific processing protease, is a downstream target for ubiquitination and degradation by pVHL E3 ligase. Targeted degradation of VDU1 by pVHL could be crucial for regulating the ubiquitin-proteasome degradation pathway."

sparser

"We next examined how CDK1-mediated phosphorylation of USP33 affects its ability to stabilize SIN1."

40695806

➶

sparser

"As shown in Fig. xref , phosphorylation of USP33 was detected in PANC-1 cells using a phospho-CDK substrate antibody."

40695806

➶

sparser

"A recent study revealed that AKT regulates the desensitization of D 2 -like receptors by phosphorylating the deubiquitinase USP33 [ xref ]."

38566235

➶

sparser

"The phosphorylated Akt subsequently phosphorylated USP33, which was recruited to the D 3 R under desensitization conditions (Figs. xref and xref )."

36658650

➶

sparser

"These results suggest that CDK1-mediated phosphorylation of USP33 is essential for its activity to regulating the ubiquitination and degradation of SIN1."

40695806

➶

sparser

"To assess the functional consequence of CDK1-mediated phosphorylation of USP33, we examined its role in SIN1-dependent malignant processes."

40695806

➶

sparser

"These results demonstrate that CDK1-mediated phosphorylation of USP33 is crucial for stabilizing SIN1 and promoting SIN1-dependent tumor progression in PDAC."

40695806

➶

sparser

"We show that CDK1-catalyzed phosphorylation of USP33 is a critical post-translational modification controlling SIN1 stability and its oncogenic activity."

40695806

USP33 is phosphorylated on S650. 3 / 3

| 3

➶

sparser

"CDK1 binds and phosphorylates USP33 at Ser650 and Ser843."

40695806

➶

sparser

"These results suggest that CDK1 regulates the phosphorylation of USP33 at Ser650 and Ser843."

40695806

➶

sparser

"First, we demonstrate that CDK1 binds and phosphorylates USP33 at Ser650 and Ser843, and the phosphorylation-deficient 2A mutation of USP33 failed to undergo phosphorylation (Fig. xref and Supplementary Fig. xref )."

40695806

USP33 is phosphorylated on S843. 3 / 3

| 3

➶

sparser

40695806

➶

sparser

"These results suggest that CDK1 regulates the phosphorylation of USP33 at Ser650 and Ser843."

40695806

➶

sparser

"CDK1 binds and phosphorylates USP33 at Ser650 and Ser843."

40695806

USP33 is phosphorylated on S439. 2 / 2

2 |

➶

hprd

No evidence text available

18669648

➶

hprd

No evidence text available

18669648

USP33 is ubiquitinated.

| 6

USP33 is ubiquitinated. 6 / 6

| 6

➶

sparser

11739384

➶

sparser

"Conversely, it has been reported that β-TrCP promotes the ubiquitination and degradation of deubiquitinase USP33 independently of the DSG motif, despite with the canonical degron motif DSGxxS [ xref ]."

40367710

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"Unexpectedly, we found that overexpression of β-TrCP can also increase the ubiquitination of FH-USP33(△DSG) ( Fig. 2 D ), and knockdown of β-TrCP also decreased the ubiquitin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

➶

sparser

"Here, our study for the first time uncovered that β-TrCP plays the roles in tumor cell proliferation and invasion by promoting ubiquitination and degradation of USP33."

28506875

➶

sparser

"USP33 was found to be ubiquitinated in a VHL-dependent manner which targeted it for proteasomal degradation, and VHL mutations that disrupt the protein’s interaction with the DUB abrogated USP33 ubiquitination."

18687060

➶

sparser

"Data from the literature indicate that pVHL30 mediates the ubiquitination and degradation of both USP33 and USP20, in turn down-regulating the pathways controlled by these two proteins [ xref ]."

35205757

ARRB2 affects USP33

9 | 4 10

ARRB2 binds USP33. 10 / 23

9 | 4 9

➶

sparser

23764054

➶

biogrid

No evidence text available

19363159

➶

biogrid

No evidence text available

19363159

➶

biogrid

No evidence text available

19363159

➶

sparser

"The immunoprecipitation assay showed that USP33 can interact with β-arrestin2 in an agonist-dependent manner (Figure xref )."

27835898

➶

biogrid

No evidence text available

27835898

➶

biogrid

No evidence text available

24377933

➶

biogrid

No evidence text available

24377933

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No evidence text available

19363159

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reach

"Upon beta 2 AR activation, the recruited beta-arrestins are rapidly ubiquitinated and deubiquitinated, and this is facilitated by a high-affinity interaction between beta-arrestin2 and USP33."

21680031

AR binds ARRB2 and USP33. 1 / 1

| 1

➶

sparser

23764054

USP33 affects TGFBR2

5 | 15 3

USP33 binds TGFBR2.

5 | 1 3

USP33 binds TGFBR2. 8 / 8

5 | 3

➶

biogrid

No evidence text available

37322017

➶

biogrid

No evidence text available

37322017

➶

sparser

"Our previous experiments proved that USP33 interacted with TGFBR2, while the effect of USP33 in TGFBR2 was unclear."

37322017

➶

sparser

"Mechanistically, USP33 interacted with TGFBR2 through DUSP1 domain of USP33, moreover, USP33 enhanced the protein stability of TGFBR2 by removing the K63-linked ubiquitin chains on TGFBR2."

37322017

➶

biogrid

No evidence text available

37322017

➶

sparser

"Further Co-IP experiments showed a similar result with our luciferase complementation assay, TGFBR2 and USP33 could bind to each other while the other candidates had no interaction with USP33 (Fig. xref )."

37322017

➶

biogrid

No evidence text available

37322017

➶

biogrid

No evidence text available

37322017

USP33 binds TGFBR2 and DUSP1 domain. 1 / 1

| 1

➶

reach

"All in all, our data revealed that USP33 regulated the K63 deubiquitination of TGFBR2 and promoted the its protein stability, meanwhile, the binding between USP33 and TGFBR2 was dependent on the DUSP1 domain of USP33."

37322017

USP33 activates TGFBR2.

| 6

USP33 activates TGFBR2. 6 / 6

| 6

➶

reach

"The IF assay showed that after the overexpression of USP33, the colocalization of TGFBR2 with lysosome was impaired, meanwhile USP33 overexpression promoted the accumulation of TGFBR2 in cell membrane (Fig. 5K)."

37322017

➶

reach

"Moreover, our data showed that USP33 promoted the recycling of TGFBR2 to cell membrane in PC cells during its internalization (Fig. S2)."

37322017

➶

reach

"In conclusion, our study suggested that USP33 prevented the degradation of TGFBR2 by lysosome by promote its recycling by the recycling endosome."

37322017

➶

reach

"Mechanistically, USP33 interacted with TGFBR2 through DUSP1 domain of USP33, moreover, USP33 enhanced the protein stability of TGFBR2 by removing the K63-linked ubiquitin chains on TGFBR2."

37322017

➶

reach

"Mechanistically, USP33 deubiquitinated and stabilized the TGFBR2 protein in PC cells and therefore enhanced the signaling of TGF-β pathway, USP33 removed the K63-linked ubiquitin conjugates from TGFBR2 and prevented its degradation by lysosome, meanwhile, USP33 promoted the recycling of TGFBR2 to cell membrane and eventually enhanced the signaling in TGF-β pathway."

37322017

➶

reach

"USP33 promoted the recycling of TGFBR2."

37322017

USP33 deubiquitinates TGFBR2.

| 4

USP33 deubiquitinates TGFBR2. 4 / 4

| 4

➶

reach

"Liu et al. reported that USP33 expression was increased among PC samples, which was correlated with poor survival of patients with PC; they found that USP33 could promote PC cell growth, migration, and invasion by triggering the deubiquitination of TGFBR2 (Liu et al. 2023)."

39589547

➶

reach

"The USP33-regulated deubiquitination of TGFBR2 avoided its lysosomal degradation, meanwhile USP33 promoted the colocalization of TGFBR2 with recycling endosome which led to its accumulation on the membrane and the activation of TGFβ signaling."

37322017

➶

reach

"Mechanistically, USP33 triggered the deubiquitination of TGFBR2 and prevented its degradation by lysosome, therefore promoted TGFBR2 accumulation in cell membrane and eventually contributed to the sustained activation of TGF-beta signaling."

37322017

➶

reach

"Our results demonstrated that the K63R-Ub mutant and USP33 co-transfection had no effect on TGFBR2 ubiquitination, while the co-transfection of USP33 with other Ub mutants impaired the ubiquitination of TGFBR2 (Fig. 6E)."

37322017

USP33 increases the amount of TGFBR2.

| 2

USP33 increases the amount of TGFBR2. 2 / 2

| 2

➶

reach

"The IHC results of xenografted tumor showed that USP33 depletion inhibited the expression of Ki67, PCNA, TGFBR2 and ZEB1 (Fig. 8G, H)."

6 2 | 6 7

VHL binds USP33. 10 / 19

6 1 | 6 6

➶

sparser

"The USP33-VHL interaction may have clinical significance because naturally occurring mutations that have been found in the beta-domain of USP33 disrupt the interaction between the two proteins."

18687060

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reach

12056827

➶

reach

12056827

➶

biogrid

No evidence text available

19706539

➶

biogrid

No evidence text available

19706539

➶

reach

12056827

➶

sparser

19571111

➶

reach

11739384

➶

biogrid

No evidence text available

11739384

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19118533

VHL binds USP33 and USP20. 1 / 1

| 1

➶

sparser

"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

12056827

ELOC binds USP33, ELOB, and VHL. 1 / 1

1 |

➶

hprd

No evidence text available

11739384

USP33 deubiquitinates VHL.

| 1

USP33 deubiquitinates ubiquitinated VHL. 1 / 1

| 1

➶

reach

"Can VDU1 or VDU2 deubiquitinate the ubiquitinated pVHL to salvage it from degradation on certain conditions?"

12056827

USP20 affects USP33

2 | 7 12

USP20 binds USP33.

2 | 6 12

USP20 binds USP33. 10 / 13

2 | 6 4

➶

biogrid

No evidence text available

23486064

➶

biogrid

No evidence text available

23486064

➶

sparser

"USP33 and USP20 likely interact with the ER membrane bilayer via their amphipathic helices since they do not have transmembrane domains [ xref ]."

35357021

➶

reach

"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."

38874156

➶

reach

12056827

➶

reach

12056827

➶

reach

23486064

➶

reach

12056827

➶

sparser

"Two DUBs, USP33 (or VDU-1) and USP20 (or VDU-2), interact with and regulate D2 [ xref ]."

29892818

➶

reach

"Interestingly, several naturally occurring mutations in the VDU-binding domain of pVHL were found to disrupt the interaction between VDU1/VDU2 and pVHL, thus suggesting a functional relevance in pVHL-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

17961905

GroEL2 binds USP33 and USP20. 4 / 4

| 4

➶

sparser

"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

24874852

➶

sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

➶

sparser

"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."

24874852

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

➶

sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

➶

sparser

23486064

VHL binds USP33 and USP20. 1 / 1

| 1

➶

sparser

"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

12056827

ARs binds USP33 and USP20. 1 / 1

| 1

➶

sparser

"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."

23764054

USP20 activates USP33.

| 1

USP20 activates USP33. 1 / 1

| 1

➶

reach

"The closely related DUBs, USP20 and USP33, deubiquitylate activated beta-adrenergic receptors as well as the adaptor and signalling protein beta-arrestin (in the case of USP33), to favour recycling from endosomes, despite largely being associated with the secretory pathway 75, 76, 77."

28064438

USP33 affects Neoplasm Metastasis

| 1 20

USP33 activates Neoplasm Metastasis.

| 1 17

USP33 activates Neoplasm Metastasis. 10 / 18

| 1 17

➶

reach

"Functionally, our experiments demonstrate that USP33 significantly enhances ovarian cancer proliferation and metastasis in a CBX2-dependent manner."

39256572

➶

reach

"In conclusion, our study found that USP33 contributed to the proliferation and metastasis of pancreatic cancer through a positive feedback loop with TGF-beta signaling pathway."

37322017

➶

reach

"For example, a recent study revealed that enhanced USP33 expression was associated with reduced survival rates in OC patients; mechanistically, USP33 promotes OC cell growth and metastasis via deubiquitinating and stabilizing CBX2 (Chen et al. 2024)."

39589547

➶

reach

"For example, in hepatocellular carcinoma (HCC), the deubiquitinase USP33 stabilizes Sp1, leading to increased c-Met expression and enhanced HCC metastasis (29)."

40125551

➶

reach

"We found that USP33 knockdown inhibited the invasion and metastasis in HCC cells both in vitro and in vivo, which was partly dependent on c-Met."

32835698

➶

reach

"Our results reveal that USP33 acts as the deubiquitinating enzyme of SP1 and contributes to HCC invasion and metastasis through activation of the SP1 and c-Met axis."

32835698

➶

reach

"USP33/TGFBR2 axis promoted the tumor growth and metastasis of PC in vivo."

37322017

➶

reach

"The overexpression of USP33 accelerated the proliferation, invasion and metastasis of PC in vitro and in vivo."

37322017

➶

reach

"Additionally, results from in vivo and in vitro studies showed that USP33 knockdown inhibits invasion, migration, and metastasis in HCC cells."

35924961

➶

reach

"USP33 is an integrin alpha6 deubiquitinase and promotes esophageal squamous cell carcinoma cell migration and metastasis."

39589547

USP33 inhibits Neoplasm Metastasis.

| 3

USP33 inhibits Neoplasm Metastasis. 3 / 3

| 3

➶

reach

"USP33 silencing can promote the migration, invasion and metastasis of lung adenocarcinoma cells in vitro, and the mechanism may involve IL-6 and SLIT2 and ROBO1 signaling pathways."

30187867

➶

reach

"USP33 knockdown significantly upregulated tumor cell proliferation and metastasis."

29802710

➶

reach

"[USP33 suppresses lung adenocarcinoma lung cell invasion and metastasis by down-regulating SLIT2 and ROBO1 signaling pathway]."

30187867

USP33 affects MYC

2 1 | 11 6

USP33 binds MYC.

2 | 6 6

USP33 binds MYC. 10 / 14

2 | 6 6

➶

sparser

"IP experiments showed that USP33 interacted with c-Myc ( xref ), and USP33 knockdown profoundly enhanced c-Myc ubiquitination ( xref )."

33718387

➶

sparser

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

biogrid

No evidence text available

33718387

➶

sparser

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

sparser

"USP33 can bind to c-Myc."

36966306

➶

reach

"USP33 could bind and deubiquitinate c-Myc."

33718387

➶

reach

"IP experiments showed that USP33 interacted with c-Myc (XREF_FIG), and USP33 knockdown profoundly enhanced c-Myc ubiquitination (XREF_FIG)."

33718387

➶

reach

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

reach

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

sparser

"Clinically, high USP33 expression correlates with poor prognosis, suggesting that the USP33-c-Myc axis may serve as both a prognostic biomarker and a potential therapeutic target."

40532745

USP33 increases the amount of MYC.

| 3

USP33 increases the amount of MYC. 2 / 2

| 2

➶

reach

"In addition, the protein levels of α-SMA, COL1A2, LDHA, and c -Myc were significantly reduced by Usp33 knockdown in primary hepatic stellate cells ( Fig. 3 F and G)."

37267862

➶

reach

"Here, our data showed knockdown of USP33 significantly decreased ECAR, the release of lactic acid, ATP synthase, and expression of c -Myc and LDHA in primary hepatic stellate cells, suggesting the gly[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

5 1 | 10 1

USP33 deubiquitinates PRKN.

1 | 7

USP33 deubiquitinates PRKN. 4 / 4

1 | 3

➶

reach

"USP33 deubiquitinates the E3 ubiquitin‐protein ligase parkin (PRKN)."

35924961

➶

reach

"USP33 deubiquitinates PRKN and parkin and antagonizes its role in mitophagy."

31432739

➶

reach

"Cellular and in vitro assays illustrated that USP33 deubiquitinates PRKN in a DUB activity dependent manner."

31432739

➶

ubibrowser

"Our findings convincingly demonstrate that USP33 is a novel PRKN deubiquitinase antagonizing its regulatory roles in mitophagy and SH-SY5Y neuron-like cell survival"

31432739

USP33 deubiquitinates PRKN on K435. 4 / 4

| 4

➶

reach

"USP33 prefers to remove K6, K11, K48 and K63 linked ubiquitin conjugates from PRKN, and deubiquitinates PRKN mainly at Lys435."

31432739

➶

reach

"USP33 Deubiquitinates K63-Linked Ubiquitin on Parkin at Lys435."

36831318

➶

reach

"USP33 deubiquitinates Lys435 of parkin and inhibits its mediated clearance of damaged mitochondria."

36260224

➶

reach

"Different from USP30 which regulates substrates of Parkin, USP33 can directly and selectively deubiquitinate Parkin at Lys435 and preferentially remove K6, K11, K48, and K63-linked ubiquitin conjugates from Parkin."

34804365

USP33 binds PRKN.

5 | 1

PRKN binds USP33. 6 / 6

5 | 1

➶

biogrid

No evidence text available

31432739

➶

sparser

"Additionally, USP33 directly interacts with parkin, and the knockdown of USP33 increases K63-linked ubiquitination of parkin under CCCP treatment, thereby enhancing mitophagy (Niu et al. xref )."

41387651

➶

biogrid

No evidence text available

31432739

➶

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No evidence text available

31432739

➶

3 | 5 3

USP33 binds CCP110. 9 / 13

3 | 5 1

➶

biogrid

No evidence text available

23486064

➶

reach

"Alternatively, excessive amounts of USP33-DM might bind CP110, blocking it from accessing SCF Cyclin F."

23486064

➶

sparser

"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain ( xref )."

23486064

➶

reach

23486064

➶

biogrid

No evidence text available

23486064

➶

reach

"USP33 interacts with CP110 and localizes to centrioles primarily during S and G2/M phase, the period during which centrioles duplicate and elongate."

23486064

➶

reach

23486064

➶

reach

"We investigated the interaction between USP33 and CP110 and found that CP110 binds to a region of USP33 within its catalytic domain (XREF_FIG)."

23486064

➶

biogrid

No evidence text available

23486064

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

➶

sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

➶

sparser

23486064

CCP110 inhibits USP33.

| 1

CCP110 inhibits USP33. 1 / 1

| 1

➶

reach

"We found that centrosomal targeting of USP33 is partially dependent on CP110 because depletion of CP110 reduced USP33 localization to centrosomes (see below) (XREF_SUPPLEMENTARY)."

23486064

USP33 affects cell migration

| 14

USP33 activates cell migration.

| 11

USP33 activates cell migration. 10 / 12

| 11

➶

reach

"These findings are the first to describe that USP33 promoted laminin-dependent cell migration by stabilizing integrin α6, which provides an insight into the mechanistic roles of USP33-integrin α6 axis in ESCC metastasis."

39589547

➶

reach

"Deficiency of HIF1A or USP33 suppressed cell migration, proliferation and microtubule formation of hypoxia-exposed HRECs."

39320536

➶

reach

"Expression of the C163A-mutant USP33 abolished Slit suppression of H1299 cell migration, supporting that deubiquitinating activity of USP33 is important in mediating Slit activity in inhibiting lung cancer cell migration."

24981056

➶

reach

"Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT."

38487955

➶

reach

"Moreover, we demonstrated that USP33 could mediate ESCC cell migration and metastasis through integrin α6."

39589547

➶

reach

"USP33 is an integrin alpha6 deubiquitinase and promotes esophageal squamous cell carcinoma cell migration and metastasis."

39589547

➶

reach

"Interestingly, overexpression of USP33 significantly increased the cell migration (Fig. 3C) but not the proliferation (Fig. 3B) ability upon laminin coating."

39589547

➶

reach

"Mechanistically, USP33 mediates cell migration through binding, deubiquinating, and stabilizing integrin alpha6."

39589547

➶

reach

"USP33 knockdown could inhibit ESCC cell migration and metastasis majorly through integrin alpha6."

39589547

➶

reach

"We found that integrin α6 and USP33 overexpression in USP33-knockdown cells can largely rescue cell migration abilities on laminin in both KYSE-150 and KYSE-450 cells compared to indicated knockdown cells (Fig. 5C), which highlights the importance of integrin α6 in USP33-mediated ESCC cell migration on laminin."

39589547

USP33 inhibits cell migration.

| 3

USP33 inhibits cell migration. 3 / 3

| 3

➶

reach

"As examples, USP33 was down-regulated in lung cancer patients and correlated with poor prognosis, inhibiting lung cancer cell migration through stabilization of the Robo1 protein (Wen et al. 2014)."

39589547

➶

reach

"In addition, USP33 also inhibits breast cancer cell migration via regulating Slit signaling [ 35 ]."

37267862

➶

reach

"Similarly, the downregulation of USP33 (a ubiquitin ligase enzyme) reduces the stability of the ROBO1 receptor in lung cancer cells 29 and breast cancer cell migration 30."

33318575

GroEL2 affects USP33

| 6 8

GroEL2 binds USP33. 10 / 10

| 6 4

➶

reach

"The results indicate that the selenium is required for the hSP56 and VDU1 interaction.We compared selenium binding to hSP56 in non reducing or reducing SDS-PAGE to determine the nature of the interact[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19118533

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sparser

"The full-length VDU1 interacts specifically with the selenium-replete form of hSP56 (hSP56/Se) in vitro , strongly suggesting that a physiological partner of VDU1 may be hSP56/Se ( Fig. 2 C)."

19118533

➶

sparser

"Notably, the results indicate that the interaction between hSP56 and VDU1 is direct, since purified proteins were used ( Fig. 1 B and C)."

19118533

➶

reach

"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA and anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm (XREF_FIG D)."

24874852

➶

sparser

"We then examined the interaction of the full-length VDU1 and hSP56 without or with pre-incubation of hSP56 with selenium."

19118533

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reach

"HSP56 interacts with both VDU1 and VDU2."

24874852

➶

sparser

"HSP56 interacted strongly with VDU1 f , compared to no interaction with GST alone or a trivial interaction with GST-β2m ( Fig. 1 B)."

19118533

➶

reach

"HSP56 interacted strongly with VDU1 f, compared to no interaction with GST alone or a trivial interaction with GST-beta2m."

19118533

➶

reach

"Notably, the results indicate that the interaction between hSP56 and VDU1 is direct, since purified proteins were used."

19118533

➶

reach

"HSP56 and VDU1 were present together in the cytoplasm as demonstrated in the merged images, consistent with physiologically relevant interactions.We then examined the interaction of the full-length VD[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

19118533

GroEL2 binds USP33 and USP20. 4 / 4

| 4

➶

sparser

24874852

➶

sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

➶

sparser

24874852

MYC affects USP33

2 | 6 6

USP33 binds MYC. 10 / 14

2 | 6 6

➶

sparser

"IP experiments showed that USP33 interacted with c-Myc ( xref ), and USP33 knockdown profoundly enhanced c-Myc ubiquitination ( xref )."

33718387

➶

sparser

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

biogrid

No evidence text available

33718387

➶

sparser

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

sparser

"USP33 can bind to c-Myc."

36966306

➶

reach

"USP33 could bind and deubiquitinate c-Myc."

33718387

➶

reach

"IP experiments showed that USP33 interacted with c-Myc (XREF_FIG), and USP33 knockdown profoundly enhanced c-Myc ubiquitination (XREF_FIG)."

33718387

➶

reach

"These data suggested that USP33 may bind and deubiquitinate c-Myc in 22RV1 cells."

33718387

➶

reach

"IP experiments revealed that USP33 could bind and deubiquitinate c-Myc."

33718387

➶

sparser

"Clinically, high USP33 expression correlates with poor prognosis, suggesting that the USP33-c-Myc axis may serve as both a prognostic biomarker and a potential therapeutic target."

40532745

CDK1 affects USP33

| 14

CDK1 binds USP33.

| 7

CDK1 binds USP33. 7 / 7

| 7

➶

sparser

"CDK1 binds and phosphorylates USP33 at Ser650 and Ser843."

40695806

➶

sparser

"Specifically, we demonstrate a previously uncharacterized tumor-promoting activity of the CDK1–USP33 axis in PDAC through stabilizing SIN1, a key mediator of mTORC2-AKT activation."

40695806

➶

sparser

"In conclusion, our study provides compelling preclinical evidence that targeting the CDK1–USP33 axis may effectively destabilize SIN1, thereby inhibiting tumor growth and overcoming gemcitabine resistance in PDAC."

40695806

➶

sparser

"Moreover, our preclinical data demonstrate that targeting the CDK1-USP33 axis effectively suppresses tumor progression in PDAC via destabilizing SIN1 (Fig. xref )."

40695806

➶

sparser

"Our results provide compelling preclinical evidence that targeting the CDK1–USP33 axis may offer a promising therapeutic strategy to destabilize SIN1 and overcome chemoresistance in PDAC and potentially other aggressive cancers."

40695806

➶

sparser

40695806

➶

sparser

"In a cell-free system, purified GST-USP33, but not GST or GST-SIN1 protein, bound to His-CDK1, suggesting a direct interaction between CDK1 and USP33 (Fig. xref )."

40695806

CDK1 phosphorylates USP33.

| 6

CDK1 phosphorylates USP33. 6 / 6

| 6

➶

sparser

40695806

➶

sparser

"As shown in Fig. xref , active CDK1 phosphorylated GST-fused USP33 WT in vitro, while the 2A mutant exhibited significantly reduced phosphorylation."

40695806

➶

sparser

"CDK1 binds and phosphorylates USP33 at Ser650 and Ser843."

40695806

➶

sparser

"We next explored whether CDK1 may phosphorylate USP33 and affect its tumor-promoting function in PDAC cells."

40695806

➶

sparser

"Additionally, we show that CDK1 directly phosphorylates USP33, enhancing its deubiquitinase activity toward SIN1 and driving PDAC progression."

40695806

➶

sparser

"To further confirm that CDK1 directly phosphorylates USP33, we performed an in vitro kinase assay, where GST-fused USP33 WT or the 2A mutant was incubated with active CDK1."

40695806

CDK1 activates USP33.

| 1

CDK1 activates USP33. 1 / 1

| 1

➶

sparser

"Unexpectedly, we find that cyclin-dependent kinase 1 (CDK1) directly activates USP33 to deubiquitinate SIN1, while targeting CDK1 significantly sensitizes PDAC cells to chemotherapy in a SIN1-dependent manner."

40695806

USP33 affects Pancreatic Neoplasms

| 13

USP33 activates Pancreatic Neoplasms.

| 12

USP33 activates Pancreatic Neoplasms. 10 / 12

| 12

➶

reach

"The overexpression of USP33 accelerated the proliferation, invasion and metastasis of PC in vitro and in vivo."

37322017

➶

reach

"Taking together, our results demonstrated that USP33 promoted the tumor growth and metastasis of PC in a TGFBR2-dependent manner."

37322017

➶

reach

"Moreover, we labeled EdU-positive PC cells to monitor the proliferation rate of PC cells, our data showed that EdU-positive cells was markedly reduced after USP33 downregulation while USP33 overexpression increased the proliferation rate of PC cells (Fig. 2F, G)."

37322017

➶

reach

"To assess whether USP33 affected the invasiveness and migration of PC cells, we performed the transwell migration and invasion assays and found that USP33 knockdown significantly inhibited the migration and invasiveness of PC cells while USP33 overexpression showed the opposite effect (Fig. 2H–J)."

37322017

➶

reach

"Our further experiments revealed that USP33 mediated the progression of PC cells through TGFBR2 which was a key regulator of TGFβ signaling pathway."

37322017

➶

reach

"To investigate the role of TGFBR2 in USP33-mediated PC cell malignance, we performed the corresponding rescue experiments."

37322017

➶

reach

"Through our function experiments, we found that USP33 promoted the proliferation, migration and invasion of PC in vitro and in vivo."

37322017

➶

reach

"USP33/TGFBR2 axis promoted the tumor growth and metastasis of PC in vivo."

37322017

➶

reach

"USP33 promoted the proliferation, migration and invasion of PC cells."

37322017

➶

reach

"The results showed that knockdown of USP33 inhibited the liver metastasis of PC and increased the OS of PC bearing nude mice, while the overexpression of TGFBR2 remedied this phenotype (Fig. 8D–F)."

37322017

USP33 inhibits Pancreatic Neoplasms.

| 1

USP33 inhibits Pancreatic Neoplasms. 1 / 1

| 1

➶

reach

37322017

USP33 affects E7

7 4 | 1 1

E7 binds USP33. 4 / 4

2 1 | 1

➶

reach

"The Gaussia princeps luciferase protein complementation assay shows that USP15 interacts with the key oncoprotein human papillomavirus (HPV) E6, while USP29 and USP33 bind to E7 protein, suggesting that cellular DUBs impact HPV tumorigenesis by regulating the stability of the viral proteins [45] ."

33922750

➶

virhostnet

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

E7 binds USP33. 2 / 2

2 |

➶

biogrid

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

E7 binds USP33. 2 / 2

1 1 |

➶

virhostnet

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

E7 binds USP33. 2 / 2

1 1 |

➶

biogrid

No evidence text available

28786561

➶

virhostnet

No evidence text available

28786561

USP33 binds E7. 1 / 1

1 |

➶

virhostnet

No evidence text available

28786561

USP29 binds USP33 and E7. 1 / 1

| 1

➶

sparser

33922750

E7 binds USP33. 1 / 1

1 |

➶

biogrid

No evidence text available

28786561

USP33 affects BTRC

2 | 3 8

BTRC binds USP33. 10 / 13

2 | 3 8

➶

reach

"We found that USP33 (^ DSG) can also interact with beta-TrCP."

28506875

➶

sparser

"Β-TrCP can also interact with FH-USP33(△DSG) ( Fig. 3 B ), indicating that the interaction between β-TrCP and USP33 is independent of DSG motif of USP33."

28506875

➶

sparser

"The interaction of β-TrCP with these USP33 mutants was evaluated."

28506875

➶

sparser

"Β-TrCP is able to interact with USP33, and promotes the ubiquitination and proteasomal degradation of USP33."

28506875

➶

sparser

"Our data showed that USP33 was able to interact with β-TrCP-WT, but unable to interact with β-TrCP-△WD40 ( Fig. 3 D )."

28506875

➶

sparser

"Collectively, we found that the WD40 motif of β-TrCP and 201–400 amino acid motif of USP33 are necessary for the interaction between β-TrCP and USP33."

28506875

➶

biogrid

No evidence text available

28506875

➶

biogrid

No evidence text available

28506875

➶

sparser

"We found that USP33(△DSG) can also interact with β-TrCP ( Fig. 3 B )."

28506875

➶

sparser

"Since β-TrCP promotes USP33 ubiquitin-dependent degradation, then we assessed the interaction between β-TrCP and USP33."

28506875

E7 affects USP33

7 4 | 1 1

E7 binds USP33. 4 / 4

2 1 | 1

➶

reach

33922750

➶

virhostnet

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

E7 binds USP33. 2 / 2

2 |

➶

biogrid

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

E7 binds USP33. 2 / 2

1 1 |

➶

virhostnet

No evidence text available

28786561

➶

biogrid

No evidence text available

28786561

E7 binds USP33. 2 / 2

1 1 |

➶

biogrid

No evidence text available

28786561

➶

virhostnet

No evidence text available

28786561

USP33 binds E7. 1 / 1

1 |

➶

virhostnet

No evidence text available

28786561

USP29 binds USP33 and E7. 1 / 1

| 1

➶

sparser

33922750

E7 binds USP33. 1 / 1

1 |

➶

biogrid

No evidence text available

28786561

TGFBR2 affects USP33

5 | 4 3

TGFBR2 binds USP33.

5 | 1 3

USP33 binds TGFBR2. 8 / 8

5 | 3

➶

biogrid

No evidence text available

37322017

➶

biogrid

No evidence text available

37322017

➶

sparser

"Our previous experiments proved that USP33 interacted with TGFBR2, while the effect of USP33 in TGFBR2 was unclear."

37322017

➶

sparser

"Mechanistically, USP33 interacted with TGFBR2 through DUSP1 domain of USP33, moreover, USP33 enhanced the protein stability of TGFBR2 by removing the K63-linked ubiquitin chains on TGFBR2."

37322017

➶

biogrid

No evidence text available

37322017

➶

sparser

37322017

➶

biogrid

No evidence text available

37322017

➶

biogrid

No evidence text available

37322017

USP33 binds TGFBR2 and DUSP1 domain. 1 / 1

| 1

➶

reach

37322017

TGFBR2 activates USP33.

| 2

TGFBR2 activates USP33. 2 / 2

| 2

➶

reach

"Moreover, the transwell assay showed that TGFBR2 overexpression rescued the effect of USP33 on the migration and invasion of PC cells (Fig. 4I)."

37322017

➶

reach

"Our results showed that USP33 deficiency significantly decreased the tumor growth of subcutaneous xenograft nude mice while the overexpression of TGFBR2 rescued the effect of USP33 (Fig. 8A–C)."

37322017

TGFBR2 inhibits USP33.

| 1

TGFBR2 inhibits USP33. 1 / 1

| 1

➶

reach

"Moreover, our results showed that USP33 knockdown led to a decrease in the phosphorylation and translocation of SMAD2/3, while the TGFBR2 overexpression rescued the effect of USP33 knockdown (Fig. 3K)."

37322017

USP33 affects TP53

| 2 9

USP33 binds TP53.

| 9

USP33 binds TP53. 9 / 9

| 9

➶

sparser

"We then examined the association of USP33 with p53 under DNA damage condition."

39694539

➶

sparser

"In particular, DNA damage agents can enhance the association of USP33 and p53 resulting in the removal of K48 polyubiquitination on p53 and consequent protein stabilisation."

39694539

➶

sparser

"To further verify the interaction between p53 and USP33, 293T whole cell lysates were incubated with control IgG or anti‐USP33 antibody, followed by Western blotting analysis on the pull‐down complex."

39694539

➶

sparser

"The result showed that the interaction between USP33 and p53 was substantially enhanced upon cisplatin, CPT or Dox treatment (Figure xref )."

39694539

➶

sparser

"Consistently, the interaction between USP33 and p53 showed a marked enhancement after CPT treatment (Figure xref )."

39694539

➶

sparser

"In an effort to search for the downstream effector of USP33, we found p53 protein level was substantially decreased upon USP33 knockdown, which drives us to explore the association of USP33 and p53 under pathophysiological conditions."

39694539

➶

sparser

"We then performed GST‐pulldown assay, and the results confirmed a direct interaction between p53 and USP33 (Figure xref )."

39694539

➶

sparser

"We further determined which domains of p53 interacts with USP33 by constructing different functional domains of Flag‐p53‐N, Flag‐p53‐M and Flag‐p53‐C, and found that both Flag‐p53‐N, Flag‐p53‐M, instead of Flag‐p53‐C, interact with USP33 (Figure xref )."

2 | 1 3 1

USP33 binds DUSP1. 5 / 5

2 | 1 2

➶

biogrid

No evidence text available

31857702

➶

biogrid

No evidence text available

31857702

➶

reach

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

➶

sparser

"USP33 directly bound dual specificity protein phosphatase 1 (DUSP1) and reduced its ubiquitination, thereby impairing JNK activation and apoptosis in prostate cancer [ xref ]."

39694539

➶

sparser

"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

USP33 binds DUSP1. 1 / 1

| 1

➶

| 7

MiR-365a-3p activates USP33. 7 / 7

| 7

➶

reach

29743814

➶

reach

"This suggested that miR-365a-3p directly targets and binds the USP33 3 ' UTR."

29743814

➶

reach

"Functional rescue assays demonstrated that miR-365a-3p promoted proliferation, migration, and invasion by downregulating USP33."

29743814

➶

reach

"This further suggested that miR-365a-3p promotes the proliferation of lung cancer cells by downregulating USP33."

29743814

➶

reach

"The above series of in vitro experiments suggested that miR-365a-3p promotes lung cancer progression by downregulating USP33; however, the signalling pathways involved in this process remain unknown."

29743814

➶

| 2 8

➶

sparser

"The result showed that USP33 could interact with integrin α6 and β4 (Fig. xref B)."

39589547

➶

sparser

"Altogether, these results indicate that USP33 could bind and stabilize integrin α6 through deubiquitination."

39589547

➶

reach

"The interaction between USP33 and integrins was detected by immunoprecipitation, and the deubiquitination was performed by deubiquitination assay."

39589547

➶

sparser

"USP33 binds and stabilizes integrin α6 through deubiquitination."

39589547

➶

sparser

"As described above, USP33 could bind and deubiquitinate integrin α6 to stabilize its expression; it is reasonable to infer that the USP33-integrin α6 axis plays an essential role in ESCC cell migration on laminin."

39589547

➶

sparser

"In addition, we detected the effect of USP33-integrin α6 regulation on cell metastasis in vivo."

39589547

➶

reach

"USP33 binds and stabilizes integrin alpha6 through deubiquitination."

39589547

➶

sparser

"The interaction between USP33 and integrins was detected by immunoprecipitation, and the deubiquitination was performed by deubiquitination assay."

39589547

➶

sparser

"Mechanistically, USP33 can interact with integrin α6 and subsequently promote its deubiquitination and stabilization."

39589547

➶

sparser

39589547

USP33 affects ARRB

| 9

USP33 binds ARRB. 8 / 8

| 8

➶

sparser

"Thus, it could be speculated that AKT exerts two distinct effects on the homologous desensitization of GPCRs: one mechanism involves AKT regulating receptor desensitization by promoting the nuclear entry of Mdm2, while an alternative mechanism involves the promotion of the interaction of USP33 with β-arrestin."

38566235

➶

sparser

"The detailed steps to assay the kinetics of β-arrestin–USP33 interaction are described later."

24377933

➶

sparser

"Thus, USP33-beta-arrestin interaction is a key regulatory step in 7TMR trafficking and signal transmission from the activated receptors to downstream effectors."

19363159

➶

sparser

"Conversely, stimulation of the vasopressin V2R, a Class B receptor known to have prolonged interaction with β-arrestins, does not enhance β-arrestin 2-USP33 interaction."

21476968

➶

sparser

"These DUBs are constitutively bound to the cell-surface β 2 ARs; however, β 2 AR–USP association decreases upon agonist activation, while simultaneously agonist stimulation leads to the recruitment of[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

23764054

➶

sparser

"Specific conformations of β-arrestins induced by different 7TMR binding can also determine the kinetics and stability of interaction of β-arrestin with the deubiquitinating enzyme USP33 [ xref ]."

21680031

➶

sparser

"Further, the association of the deubiquitinase USP33 with β-arrestin 2 was shown to regulate 7TMR-β-arrestin 2 interaction with greater USP33-β-arrestin 2 association resulting in decreased 7TMR-β-arrestin 2 interaction and loss of localized intracellular signaling [ xref ]."

21476968

➶

sparser

"The association of USP33 and β-arrestin 2 itself appears to be dependent on the class of 7TMR as activation of the β2-adrenergic receptor (β2AR), a Class A receptor known to have a transient interaction with β-arrestins, enhances the association of β-arrestin 2 with USP33 [ xref ]."

21476968

USP33 binds ARRB and 7TMR. 1 / 1

| 1

➶

sparser

21476968

TP53 affects USP33

| 9

USP33 binds TP53. 9 / 9

| 9

➶

sparser

"We then examined the association of USP33 with p53 under DNA damage condition."

39694539

➶

sparser

"In particular, DNA damage agents can enhance the association of USP33 and p53 resulting in the removal of K48 polyubiquitination on p53 and consequent protein stabilisation."

39694539

➶

sparser

39694539

➶

sparser

"The result showed that the interaction between USP33 and p53 was substantially enhanced upon cisplatin, CPT or Dox treatment (Figure xref )."

39694539

➶

sparser

"Consistently, the interaction between USP33 and p53 showed a marked enhancement after CPT treatment (Figure xref )."

39694539

➶

sparser

39694539

➶

sparser

"We then performed GST‐pulldown assay, and the results confirmed a direct interaction between p53 and USP33 (Figure xref )."

39694539

➶

sparser

39694539

➶

sparser

"The findings of USP33 directly interacting with p53 drove us to determine whether USP33 deubiquitinates p53 and further regulates its stability."

39694539

ARRB affects USP33

| 9

USP33 binds ARRB. 8 / 8

| 8

➶

sparser

38566235

➶

sparser

"The detailed steps to assay the kinetics of β-arrestin–USP33 interaction are described later."

24377933

➶

sparser

"Thus, USP33-beta-arrestin interaction is a key regulatory step in 7TMR trafficking and signal transmission from the activated receptors to downstream effectors."

19363159

➶

sparser

"Conversely, stimulation of the vasopressin V2R, a Class B receptor known to have prolonged interaction with β-arrestins, does not enhance β-arrestin 2-USP33 interaction."

21476968

➶

sparser

23764054

➶

sparser

"Specific conformations of β-arrestins induced by different 7TMR binding can also determine the kinetics and stability of interaction of β-arrestin with the deubiquitinating enzyme USP33 [ xref ]."

21680031

➶

sparser

21476968

➶

sparser

21476968

USP33 binds ARRB and 7TMR. 1 / 1

| 1

➶

sparser

21476968

USP33 affects SELENBP1

2 | 2 2

SELENBP1 binds USP33. 6 / 8

2 | 2 2

➶

reach

"SELENBP1 has also been implicated in ubiquitination from a study demonstrating the binding of SELENBP1 to von Hippel- Lindau protein (pVHL) - interacting deubiquitinating enzyme 1 (VDU1) that was abolished with the incubation of beta-mercaptoethanol, which dissociates selenium, suggesting a selenium dependent interaction of SELENBP1 and VDU1."

23805071

➶

reach

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells (Jeong et al., 2009), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

➶

sparser

"SELENBP1 has also been implicated in ubiquitination from a study demonstrating the binding of SELENBP1 to von Hippel- Lindau protein (pVHL)- interacting deubiquitinating enzyme 1 (VDU1) that was abolished with the incubation of β-mercaptoethanol, which dissociates selenium (Jeong et al., xref ), suggesting a selenium dependent interaction of SELENBP1 and VDU1."

23805071

➶

biogrid

No evidence text available

19118533

➶

biogrid

No evidence text available

19118533

➶

sparser

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells ( xref ), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

USP33 affects IRF9

| 2 6

USP33 binds IRF9.

| 6

IRF9 binds USP33. 6 / 6

| 6

➶

sparser

"Disruption of the USP33-IRF9 axis stimulates the noncanonical activation of pro-inflammatory genes from microglia and leads to severe neuroinflammation in the central nervous system (CNS)."

36593971

➶

sparser

"Collectively, SARS-CoV-2 not only regulates the levels of circulating miRNAs activating cytokines, but also mediates the alternative pathways of CNS injury by inhibiting the USP33-IRF9 axis."

36593971

➶

sparser

"Therefore, the hyperactivation of the USP33-IRF9 axis regulated by exosomal miRNA in human microglia uncovers a bystander pathway of SARS-CoV-2-mediated CNS damage."

36977948

➶

sparser

"They also deregulated the USP33-IRF9 network following their internalization in human microglia cells."

36077138

➶

sparser

"Disruption of USP33-IRF9 axis stimulate the non-canonical activation of pro-inflammatory genes from microglia and lead to severe neuroinflammation inside CNS ( xref )."

2 | 2 2

➶

reach

23805071

➶

reach

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells (Jeong et al., 2009), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

➶

sparser

"SELENBP1 has also been implicated in ubiquitination from a study demonstrating the binding of SELENBP1 to von Hippel- Lindau protein (pVHL)- interacting deubiquitinating enzyme 1 (VDU1) that was abolished with the incubation of β-mercaptoethanol, which dissociates selenium (Jeong et al., xref ), suggesting a selenium dependent interaction of SELENBP1 and VDU1."

23805071

➶

biogrid

No evidence text available

19118533

➶

biogrid

No evidence text available

19118533

➶

sparser

"The interaction between USP33 and SELENBP1 was detected by a two-hybrid assay in human prostate cancer cells ( xref ), indicating SELENBP1 may play a role in epigenetics regulation."

36386843

AKT affects USP33

| 3 5

AKT binds USP33.

| 1 4

AKT binds USP33. 5 / 5

| 1 4

➶

reach

"We observed that whereas the interaction between Akt and USP33 increased under the desensitization conditions (Fig. 6A), this response was absent when we used USP33 harboring a mutated T154 residue, which is a known consensus Akt phosphorylation site (Fig. 6B)."

36658650

➶

sparser

"The desensitization-induced increase in the interaction between Akt and USP33 (Fig. xref A), and the nuclear translocation of Gβγ and arrestin (Fig. xref B) were also inhibited by pretreatment with AG1478."

39468452

➶

sparser

"Indeed, biochemical changes in cellular components constituting the GPCR desensitization cascade were evoked when cells were treated with EGF, such as phosphorylation of Src (Fig. xref A), Akt (Fig. xref B), the interaction between Akt and USP33 (Fig. xref C), which culminated in the deubiquitination of arrestins (Fig. xref D)."

39468452

➶

| 1 3

➶

sparser

"Protein interaction between USP33 and TRAF3 was identified by immunofluorescence staining."

39637362

➶

sparser

"Mechanistically, USP33 interacted with TRAF3 and induced TRAF3 deubiquitination to up-regulate its expression (P < 0.05)."

39637362

➶

sparser

"Moreover, USP33-TRAF3 activated the NF-κB pathway (P < 0.05)."

39637362

➶

reach

"Protein interaction between USP33 and TRAF3 was identified by immunofluorescence staining.Results: Caerulein dose-dependently induced apoptosis, oxidative stress, and inflammatory response in HPDE6-C7 cells and promoted macrophage M1 polarization to enhance inflammation (P < 0.05)."

39637362

USP33 deubiquitinates TRAF3.

| 3

USP33 deubiquitinates TRAF3. 3 / 3

| 3

➶

reach

"USP33 promotes caerulein-induced apoptotic, oxidative and inflammatory injuries in acute pancreatitis by deubiquitinating TRAF3."

39637362

➶

reach

"Conclusion: USP33 promoted caerulein-induced apoptosis, oxidative stress and inflammation in pancreatic ductal cells by deubiquitinating TRAF3, indicating a novel insight into the pathogenesis of AP."

39637362

➶

reach

"Mechanistically, USP33 interacted with TRAF3 and induced TRAF3 deubiquitination to up-regulate its expression (P < 0.05)."

39637362

USP33 affects JAK2

1 | 4 2

USP33 binds JAK2.

1 | 1 2

USP33 binds JAK2. 4 / 4

1 | 1 2

➶

sparser

"In the present experiment, we found for the first time that USP33 could directly interact with JAK2 and maintain its protein stability by reducing its ubiquitination modification, thus activating JAK2/STAT3 signaling pathway."

37256445

➶

sparser

"Then, we further determined the interaction between USP33 and JAK2."

37256445

➶

reach

"Then, we further determined the interaction between USP33 and JAK2."

37256445

➶

biogrid

No evidence text available

37256445

USP33 deubiquitinates JAK2.

| 3

USP33 deubiquitinates JAK2. 3 / 3

| 3

➶

reach

"From the UbiBrowser _V2 data, we found that JAK2 was a potential target for USP33, suggesting that USP33 probably participated in the deubiquitination of JAK2 protein (Fig. 5A)."

37256445

➶

reach

"Further in vivo ubiquitination assay verified that USP33 overexpression significantly decreased JAK2 ubiquitination compared with the control group (Fig. 5C)."

37256445

➶

reach

"All those results demonstrated that USP33 overexpression activated JAK2/STAT3 signaling pathway through deubiquitinating JAK2."

37256445

USP33 affects GCGR

1 | 5 1

USP33 deubiquitinates GCGR.

| 3

USP33 deubiquitinates GCGR. 3 / 3

| 3

➶

reach

"USP33 knockdown drastically increases GCGR ubiquitination in quiescent cells; one possibility is that USP33 deubiquitinates and protects newly synthesized receptors from 26S proteasomal degradation."

32967969

➶

reach

"Our data suggest that USP33 constitutively deubiquitinates the GCGR, whereas both STAMBP and USP33 deubiquitinate agonist activated GCGRs at early endosomes."

33453899

➶

reach

"Our data suggest that USP33 constitutively deubiquitinates the GCGR, whereas both STAMBP and USP33 deubiquitinate agonist-activated GCGRs at early endosomes."

32967969

USP33 binds GCGR.

1 | 1 1

GCGR binds USP33. 3 / 3

1 | 1 1

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sparser

"The USP33-GCGR complex likely dissembles at later times of agonist, as suggested by the decreasing interaction seen in the pulldown assay at 60 min of agonist stimulation ( xref , C and D )."

32967969

➶

reach

"The USP33-GCGR complex likely dissembles at later times of agonist, as suggested by the decreasing interaction seen in the pulldown assay at 60 min of agonist stimulation (Fig. 7, C and D)."

32967969

➶

biogrid

No evidence text available

32967969

USP33 activates GCGR.

| 1

USP33 activates GCGR. 1 / 1

| 1

➶

reach

"Overall, these data strongly suggest that both STAMBP and USP33 deubiquitinate agonist-activated GCGR, whereas the basal ubiquitination of GCGR is governed only by USP33 activity."

32967969

USP33 affects CXCR4

| 5

USP33 inhibits CXCR4.

| 3

USP33 inhibits CXCR4. 3 / 5

| 3

➶

reach

"On the other hand, we tested the level of total receptors after SDF-1 stimulation and demonstrated that USP33 knock-down can increase the degradation of CXCR4, while USP33 overexpression can inhibit its degradation."

27835898

➶

reach

"We demonstrated that USP33 overexpression can attenuate CXCR4 internalization, while USP33 knock-down showed the opposite effect."

27835898

➶

reach

"We also demonstrated that in the LoVo cells transfected with CXCR4, overexpression of beta-arrestin2 can further promote cell proliferation, while co-overexpression of USP33 can attenuate the oncogenetic effects of CXCR4 (XREF_SUPPLEMENTARY)."

27835898

USP33 activates CXCR4.

| 2

USP33 activates CXCR4. 2 / 2

| 2

➶

reach

"and silencing USP33 can promote internalization and degradation of CXCR4."

27835898

➶

reach

"We have proved that USP33 knock-down can promote the internalization and degradation of CXCR4."

27835898

USP33 affects CTNNB1

3 | 3 1

USP33 binds CTNNB1.

3 | 1 1

USP33 binds CTNNB1. 5 / 5

3 | 1 1

➶

sparser

"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

37076992

➶

reach

"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

37076992

➶

biogrid

No evidence text available

37076992

➶

biogrid

No evidence text available

| 7

➶

sparser

"CDK1 binds and phosphorylates USP33 at Ser650 and Ser843."

40695806

➶

sparser

"Specifically, we demonstrate a previously uncharacterized tumor-promoting activity of the CDK1–USP33 axis in PDAC through stabilizing SIN1, a key mediator of mTORC2-AKT activation."

40695806

➶

sparser

40695806

➶

sparser

"Moreover, our preclinical data demonstrate that targeting the CDK1-USP33 axis effectively suppresses tumor progression in PDAC via destabilizing SIN1 (Fig. xref )."

40695806

➶

sparser

40695806

➶

sparser

40695806

➶

sparser

"In a cell-free system, purified GST-USP33, but not GST or GST-SIN1 protein, bound to His-CDK1, suggesting a direct interaction between CDK1 and USP33 (Fig. xref )."

40695806

DUSP1 affects USP33

2 | 1 3 1

USP33 binds DUSP1. 5 / 5

2 | 1 2

➶

biogrid

No evidence text available

31857702

➶

biogrid

No evidence text available

31857702

➶

reach

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

➶

sparser

"USP33 directly bound dual specificity protein phosphatase 1 (DUSP1) and reduced its ubiquitination, thereby impairing JNK activation and apoptosis in prostate cancer [ xref ]."

39694539

➶

sparser

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USP33 binds DUSP1. 1 / 1

| 1

➶

isi

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

JNK binds USP33 and DUSP1. 1 / 1

| 1

➶

sparser

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

DIO2 affects USP33

5 1 | 1

DIO2 binds USP33.

5 1 |

DIO2 binds USP33. 6 / 6

5 1 |

➶

biogrid

No evidence text available

12865408

➶

biogrid

No evidence text available

12865408

➶

biogrid

No evidence text available

24196352

➶

hprd

No evidence text available

12865408

➶

biogrid

No evidence text available

12865408

➶

biogrid

No evidence text available

17452445

DIO2 deubiquitinates USP33.

| 1

DIO2 deubiquitinates USP33. 1 / 1

| 1

➶

reach

"DIO2 works as a dimer that associates with Hedgehog inducible ubiquitin ligase WD repeat and SOCS box containing 1 (WSB-1) [XREF_BIBR], ubiquitin conjugases UBC6 and UBC-7 [XREF_BIBR], and DIO2 specific deubiquitinating enzymes ubiquitin specific peptidase 20 (USP20), and ubiquitin specific peptidase 33 (USP33) [XREF_BIBR]."

31222623

Rep affects USP33

6 |

Rep binds USP33. 6 / 6

6 |

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

37704626

➶

biogrid

No evidence text available

34672954

➶

biogrid

No evidence text available

| DOI

USP33 affects apoptotic process

| 6

USP33 inhibits apoptotic process. 6 / 6

| 6

➶

reach

"Previous studies showed that USP33 promotes cell proliferation and reduces apoptosis in retinoblastoma [ 36 ]."

37267862

➶

reach

"More importantly, DUSP1 overexpression could reverse the USP33 knockdown induced JNK activation and apoptosis in docetaxel treated prostate cancer cells."

31857702

➶

reach

"Additionally, accumulated evidence has demonstrated that USP33 was elevated in prostates cancer and USP33 knockdown increased apoptosis of prostates cancer cells [ 37 ]."

37267862

➶

reach

"Ubiquitin specific peptidase 33 promotes cell proliferation and reduces apoptosis through regulation of the SP1/PI3K/AKT pathway in retinoblastoma."

34470581

➶

reach

"In retinoblastoma, USP33 was reported to regulate cell proliferation and inhibit apoptosis via stabilizing SP1 and activation of SP1/PI3K/Akt signaling pathway [47]."

36582601

➶

reach

"USP33 overexpression impedes paclitaxel-triggered apoptosis in resistant prostate cancer cells."

38702734

USP33 affects Slit-Robo

| 6

USP33 activates Slit-Robo. 6 / 6

| 6

➶

reach

"Recently, Huang et al. found that the USP33 mediates Slit-Robo signaling in inhibiting CRC cell migration [XREF_BIBR]."

26674478

➶

reach

"USP33 mediates Slit-Robo signaling in lung cancer cell migration."

24981056

➶

reach

"USP33, a new player in lung cancer, mediates Slit-Robo signaling."

24981056

➶

reach

"USP33 mediates Slit-Robo signaling in inhibiting colorectal cancer cell migration."

25242263

➶

reach

"USP33 can also inhibit tumor progression of colorectal cancer by targeting Slit-Robo signaling pathway or beta-arrestin signaling [XREF_BIBR, XREF_BIBR]."

29574466

➶

reach

"USP33 can regulate centrosome amplication [XREF_BIBR], recycling of beta-adrenergic receptor [XREF_BIBR, XREF_BIBR], and mediate Slit-Robo signaling in carcinoma [XREF_BIBR, XREF_BIBR]."

27835898

USP33 affects RALB

2 1 | 3

USP33 deubiquitinates RALB.

1 | 3

USP33 deubiquitinates RALB. 4 / 4

1 | 3

➶

ubibrowser

"26445063"

26445063

➶

reach

"Another example in the context of early signaling events is the deubiquitination of RALB by USP33, which enables the interaction of non modified RALB with Beclin 1 complexes in order to induce autophagy."

26445063

➶

reach

"Deubiquitylation of RALB by USP33 promotes the assembly of complexes which contain Beclin-1 and which stimulate autophagosome formation ( Simicek et al., 2013 )."

25444757

➶

reach

"Starvation induced deubiquitination of RALB by USP33 (XREF_FIG c) prevents the association with SEC5 and triggers the interaction to another exocyst subunit, EXO84 [XREF_BIBR]."

26445063

USP33 binds RALB.

2 |

RALB binds USP33. 2 / 2

2 |

➶

biogrid

No evidence text available

24056301

➶

biogrid

No evidence text available

24056301

USP33 affects FGFR2

| 6

USP33 binds FGFR2. 6 / 6

| 6

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sparser

"In the FIGHT-101 trial, a patient with KRAS WT PDAC harboring an FGFR2-USP33 fusion achieved a partial response to pemigatinib that was maintained for 10.7 months before disease progression [ xref ]."

36551707

➶

sparser

"In the FIGHT-101 clinical trial, one of five patients with pancreatic cancer showed a partial response to anti-FGFR1-3 treatment (pemigatinib); this case was positive for FGFR2::USP33 fusion [ xref ]."

38542259

➶

sparser

"C382R mutation, and FGF3,4,19 amplifications), and 1 each with gallbladder cancer ( FGFR2-BICC1 fusion), pancreatic cancer ( FGFR2-USP33 fusion), urothelial cancer ( FGFR3 p."

35176457

➶

sparser

"Following disease progression on this trial, genomic testing revealed an FGFR2-USP33 fusion, and she subsequently started a basket trial for the FGFR-selective inhibitor pemigatinib where she exhibited a partial response with a 43% reduction in tumor size (NCT02393248) and remained on therapy for 28 months (Fig. xref , Supplementary Figs. xref , xref , xref ) xref ."

39289482

➶

sparser

"One in four patients with pancreatic cancer had FGFR2-USP33 fusion and maintained a response for 10.7 months."

37893023

➶

sparser

"Before starting pemigatinib, her plasma cfDNA FGFR2-USP33 fusion was detected at 1.1% VAF and subsequently decreased to an undetectable level two weeks after starting treatment (Fig. xref )."

39289482

USP33 affects DIO2

5 1 |

DIO2 binds USP33. 6 / 6

5 1 |

➶

biogrid

No evidence text available

12865408

➶

biogrid

No evidence text available

12865408

➶

biogrid

No evidence text available

24196352

➶

hprd

No evidence text available

12865408

➶

biogrid

No evidence text available

12865408

➶

biogrid

No evidence text available

17452445

USP33 affects ARR3

1 | 5

ARR3 binds USP33. 6 / 6

1 | 5

➶

biogrid

No evidence text available

36658650

➶

reach

"As shown in Fig. 3A and B, interactions between USP33, D R, and arrestin3 remained similar under non-desensitization conditions (30 nM DA, w +), but increased under desensitization conditions (100 nM Quin, w +)."

36658650

➶

reach

"In addition, an interaction between USP33 and arrestin3 occurred in an Mdm2-dependent manner (Fig. 3C)."

36658650

➶

reach

"Immunocytochemical analysis showed that USP33 formed a complex with arrestin3 under desensitization conditions (Fig. 3D; Quin, w +)."

36658650

➶

reach

"Akt facilitates the interaction between USP33 and arrestin3."

36658650

➶

reach

"Consistent with the inhibition of arrestin3 deubiquitination by pretreatment with triciribine (Fig. 5B), this pretreatment was found to block the interaction between arrestin3 and USP33 (Fig. 6E)."

36658650

PRKN affects USP33

5 | 1

PRKN binds USP33. 6 / 6

5 | 1

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biogrid

No evidence text available

31432739

➶

sparser

"Additionally, USP33 directly interacts with parkin, and the knockdown of USP33 increases K63-linked ubiquitination of parkin under CCCP treatment, thereby enhancing mitophagy (Niu et al. xref )."

41387651

➶

biogrid

No evidence text available

31432739

➶

biogrid

No evidence text available

31432739

➶

biogrid

No evidence text available

31432739

➶

biogrid

No evidence text available

31432739

IRF9 affects USP33

| 6

IRF9 binds USP33. 6 / 6

| 6

➶

sparser

"Disruption of the USP33-IRF9 axis stimulates the noncanonical activation of pro-inflammatory genes from microglia and leads to severe neuroinflammation in the central nervous system (CNS)."

36593971

➶

sparser

"Collectively, SARS-CoV-2 not only regulates the levels of circulating miRNAs activating cytokines, but also mediates the alternative pathways of CNS injury by inhibiting the USP33-IRF9 axis."

36593971

➶

sparser

"Therefore, the hyperactivation of the USP33-IRF9 axis regulated by exosomal miRNA in human microglia uncovers a bystander pathway of SARS-CoV-2-mediated CNS damage."

36977948

➶

sparser

"They also deregulated the USP33-IRF9 network following their internalization in human microglia cells."

36077138

➶

sparser

"Disruption of USP33-IRF9 axis stimulate the non-canonical activation of pro-inflammatory genes from microglia and lead to severe neuroinflammation inside CNS ( xref )."

33936086

➶

sparser

"Mishra, R. and Banerjea, A. C., SARS-CoV-2 Spike Targets USP33-IRF9 Axis via Exosomal miR-148a to Activate Human Microglia."

| DOI

FGFR2 affects USP33

| 6

USP33 binds FGFR2. 6 / 6

| 6

➶

sparser

36551707

➶

sparser

38542259

➶

sparser

"C382R mutation, and FGF3,4,19 amplifications), and 1 each with gallbladder cancer ( FGFR2-BICC1 fusion), pancreatic cancer ( FGFR2-USP33 fusion), urothelial cancer ( FGFR3 p."

35176457

➶

sparser

39289482

➶

sparser

"One in four patients with pancreatic cancer had FGFR2-USP33 fusion and maintained a response for 10.7 months."

37893023

➶

sparser

"Before starting pemigatinib, her plasma cfDNA FGFR2-USP33 fusion was detected at 1.1% VAF and subsequently decreased to an undetectable level two weeks after starting treatment (Fig. xref )."

39289482

ARR3 affects USP33

1 | 5

ARR3 binds USP33. 6 / 6

1 | 5

➶

biogrid

No evidence text available

36658650

➶

reach

36658650

➶

reach

"In addition, an interaction between USP33 and arrestin3 occurred in an Mdm2-dependent manner (Fig. 3C)."

36658650

➶

reach

"Immunocytochemical analysis showed that USP33 formed a complex with arrestin3 under desensitization conditions (Fig. 3D; Quin, w +)."

36658650

➶

reach

"Akt facilitates the interaction between USP33 and arrestin3."

36658650

➶

reach

"Consistent with the inhibition of arrestin3 deubiquitination by pretreatment with triciribine (Fig. 5B), this pretreatment was found to block the interaction between arrestin3 and USP33 (Fig. 6E)."

36658650

Lipopolysaccharide affects USP33

| 5

Lipopolysaccharide activates USP33. 5 / 5

| 5

➶

reach

"Correction: miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway."

37432634

➶

reach

"miR-206 alleviates LPS-induced inflammatory injury in cardiomyocytes via directly targeting USP33 to inhibit the JAK2/STAT3 signaling pathway."

37256445

➶

reach

"Further RT-qPCR experiments showed that LPS stimulation induced significant downregulation of miR-206 (Fig. 1B) and remarkable upregulation of USP33 (Fig. 1C)."

37256445

➶

reach

"In addition, the activation of JAK2/STAT3 by coumermycin A1 remarkably reversed the protective role of miR-206 in LPS-induced inflammatory injuries, suggesting JAK2/STAT3 signaling was a key downstream pathway of miR-206/USP33 in SCM.In this study, our data revealed that miR-206 alleviated LPS-induced inflammatory injuries by directly targeting USP33 and suppressing the activation of JAK2/STAT3 signaling pathway."

37256445

➶

reach

"Gain-of-function of miR-206 promoted the proliferation but suppressed the inflammatory responses and apoptosis of LPS-induced cardiomyocytes by targeting USP33 and suppressing the JAK2/STAT3 signaling pathway."

37256445

USP33 affects SP1

| 1 2 2

USP33 binds SP1.

| 2 2

USP33 binds SP1. 4 / 4

| 2 2

➶

reach

"Mechanistically, USP33 directly bound SP1 and decreased its ubiquitination, thereby upregulating c-Met expression."

32835698

➶

sparser

"Intriguingly, the results indicated that endogenous USP33 and SP1 are directly bound in HCC cells ( Fig. 5 C and Supplemental Fig. 7A)."

32835698

➶

reach

"Mechanistically, USP33 directly bound SP1 protein and decrease its ubiquitination and enhance its protein stability, thereby upregulating c-Met expression which eventually promoted the migration and invasion of HCC [40]."

37322017

➶

sparser

"To this end, we first performed Co-IP experiments to detect the interaction between USP33 and SP1 proteins in HCC cells."

32835698

USP33 deubiquitinates SP1.

| 1

USP33 deubiquitinates SP1. 1 / 1

| 1

➶

trips

"USP33 regulates c-Met expression by deubiquitinating SP1 to facilitate metastasis in hepatocellular carcinoma."

32835698

USP33 affects M

4 1 |

M binds USP33. 5 / 5

4 1 |

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

37478010

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

34597346

➶

virhostnet

No evidence text available

34232536

USP33 affects GST

| 5

USP33 binds GST. 5 / 5

| 5

➶

sparser

"Moreover, purified GST-USP33, but not GST alone, interacted with purified SIN1 under cell-free conditions (Fig. xref )."

40695806

➶

sparser

"In a cell-free system, purified GST-USP33, but not GST or GST-SIN1 protein, bound to His-CDK1, suggesting a direct interaction between CDK1 and USP33 (Fig. xref )."

40695806

➶

sparser

"GST‐USP33 purified from bacteria and ubiquitinated p53 protein from 293T cells were used."

39694539

➶

sparser

"In vitro assay confirmed that purified GST-USP33 WT, rather than the inactive mutant, efficiently cleaved polyubiquitinated SIN1 (Fig. xref )."

40695806

➶

sparser

"As shown in Figure xref , GST‐USP33 effectively deubiquitinated p53 in vitro."

39694539

USP33 affects DUSP2

| 5

DUSP2 binds USP33. 5 / 5

| 5

➶

sparser

"We were successful in producing the USP4-DUSP, USP11-DUSP, USP15-DUSP, USP20-DUSP2 and USP33-DUSP2 recombinant proteins in Escherichia coli and purified them to near homogeneity from the soluble fract[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

➶

sparser

"Under native conditions, size-exclusion chromatography (SEC) demonstrated that each DUSP protein existed as a stable monomer, except USP33-DUSP2, which eluted as two peaks with one peak corresponding [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

➶

sparser

"Finally, among six UbVs selected for binding to USP33-DUSP2, three exhibited some non-specific binding to SAV (33D2.4–6) but the other three exhibited absolute specificity for their cognate target (33[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

➶

sparser

"For USP15-DUSP and USP33-DUSP2, the best UbVs exhibited affinities in the low nanomolar range (K D ∼ 10 nM), whereas for USP11-DUSP and USP20-DUSP2, affinities were in the mid-nanomolar range (K D ∼ 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

34666042

➶

sparser

34666042

USP33 affects AR

| 3 2

USP33 binds AR.

| 1 2

USP33 binds AR. 2 / 2

| 1 1

➶

sparser

"Usp33 interacts with β 2 AR but mechanistically how this occurs is not known."

23201781

➶

reach

"Usp33 interacts with beta 2 AR but mechanistically how this occurs is not known."

23201781

AR binds ARRB2 and USP33. 1 / 1

| 1

➶

sparser

23764054

USP33 deubiquitinates AR.

| 2

USP33 leads to the deubiquitination of AR. 2 / 2

| 2

➶

reach

"Ubiquitination of the beta 2 AR is reversed by the deubiquitinases USP20 and USP33, which tonically associate with the beta 2 AR."

23166351

➶

reach

"104 Two DUBs USP20 and USP33 have been shown to mediate deubiquitination of beta 2 AR."

26055058

USP33 affects AKT

| 1 4

AKT binds USP33. 5 / 5

| 1 4

➶

reach

36658650

➶

sparser

39468452

➶

sparser

39468452

➶

sparser

36658650

➶

sparser

36658650

USP33 affects ADRB2

2 1 | 2

USP33 deubiquitinates ADRB2.

1 | 1

USP33 deubiquitinates ADRB2. 2 / 2

1 | 1

➶

4 1 |

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

37478010

➶

biogrid

No evidence text available

| DOI

➶

biogrid

No evidence text available

34597346

➶

virhostnet

No evidence text available

34232536

GST affects USP33

| 5

USP33 binds GST. 5 / 5

| 5

➶

sparser

"Moreover, purified GST-USP33, but not GST alone, interacted with purified SIN1 under cell-free conditions (Fig. xref )."

40695806

➶

sparser

"In a cell-free system, purified GST-USP33, but not GST or GST-SIN1 protein, bound to His-CDK1, suggesting a direct interaction between CDK1 and USP33 (Fig. xref )."

40695806

➶

sparser

"GST‐USP33 purified from bacteria and ubiquitinated p53 protein from 293T cells were used."

39694539

➶

sparser

"In vitro assay confirmed that purified GST-USP33 WT, rather than the inactive mutant, efficiently cleaved polyubiquitinated SIN1 (Fig. xref )."

40695806

➶

sparser

"As shown in Figure xref , GST‐USP33 effectively deubiquitinated p53 in vitro."

39694539

DUSP2 affects USP33

| 5

DUSP2 binds USP33. 5 / 5

| 5

➶

sparser

34666042

➶

sparser

34666042

➶

sparser

34666042

➶

sparser

34666042

➶

sparser

34666042

CTNNB1 affects USP33

3 | 1 1

USP33 binds CTNNB1. 5 / 5

3 | 1 1

➶

sparser

"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

37076992

➶

reach

"USP33 interacts with CTNNB1, and stabilizes CTNNB1 by suppressing its degradation."

37076992

➶

biogrid

No evidence text available

37076992

➶

biogrid

No evidence text available

37076992

➶

biogrid

No evidence text available

30323974

Valproic acid affects USP33

4 |

Valproic acid decreases the amount of USP33.

3 |

Valproic acid decreases the amount of USP33. 3 / 3

3 |

➶

ctd

No evidence text available

25192806

➶

ctd

No evidence text available

23179753

➶

ctd

No evidence text available

27188386

Valproic acid increases the amount of USP33.

1 |

Valproic acid increases the amount of USP33. 1 / 1

1 |

➶

ctd

No evidence text available

17183730

GroEL2 affects USP20

| 4

GroEL2 binds USP33 and USP20. 4 / 4

| 4

➶

sparser

24874852

➶

sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

➶

sparser

24874852

ZEB1 affects USP33

| 3 1

ZEB1 increases the amount of USP33.

| 2

ZEB1 increases the amount of USP33. 2 / 2

| 2

➶

reach

"Moreover, the downstream gene of TGFβ named ZEB1 was intriguing the transcriptional regulator of USP33, we found that ZEB1 promoted the transcription of USP33 through binding to the 622-632 site of USP33 promoter."

| 4

➶

reach

39589547

➶

reach

39589547

➶

reach

"USP33 promotes laminin-dependent cell adhesion and migration."

39589547

➶

reach

"USP33 promoted laminin-dependent adhesion, spreading, and migration of ESCC cells but not their proliferation."

39589547

USP33 affects glycolytic process

| 4

USP33 activates glycolytic process. 4 / 4

| 4

➶

reach

37267862

➶

reach

"Moreover, overexpression of USP33 caused marked enhancements in glycolysis, which was reversed by c -Myc inhibitor 10058-F4."

37267862

➶

reach

"It is suggested that USP33 promotes aerobic glycolysis in OS cells and inhibits mitochondrial respiration."

38288377

➶

reach

"Those results suggested that Usp33 knockdown inhibits activation and glycolysis of primary hepatic stellate cells.Usp33 overexpression promotes activation and glycolysis of primary hepatic stellate ce[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

37267862

USP33 affects cell growth

| 4

USP33 inhibits cell growth.

| 2

USP33 inhibits cell growth. 2 / 2

| 2

➶

reach

"MTT results showed that USP33 can inhibit cellular growth (XREF_FIG)."

29802710

➶

reach

"Unlike USP33, which has been reported to inhibit mitogen activated protein kinase (MAPK) activation pathway and suppress hepatoma cell growth 26, our study suggests that USP21 activates ERK1/2 to promote HCC cell proliferation."

29706623

USP33 activates cell growth.

| 2

USP33 activates cell growth. 2 / 2

| 2

➶

reach

"Functional assays suggested that USP33 promoted OS cell growth through the induction of aerobic glycolysis."

37034227

➶

reach

39589547

USP33 affects TGFB

| 3 1

USP33 activates TGFB.

| 2

USP33 activates TGFB. 2 / 2

| 2

➶

reach

| 2

USP33 inhibits Slit. 2 / 2

| 2

➶

reach

"Downregulation of USP33 inhibits Slit and Robo signaling pathway and is associated with poor patient survival of glioma."

32972109

➶

reach

"Mechanistically, USP33 inhibits the migration of glioma cells by regulating the function of the Slit/Robo signaling pathway [76]."

40149733

USP33 activates Slit.

| 2

USP33 activates Slit. 2 / 2

| 2

➶

reach

"USP33 mediates Slit signaling in lung cancer cells, and this requires the enzymatic activity of USP33, because expression of USP33 mutant (C163A) abolishes the Slit activity in lung cancer cells."

24981056

➶

reach

"Our previous work on breast cancer and the current data from lung cancer suggest that USP33 may mediate Slit signaling in different cancer cells through different mechanisms."

24981056

USP33 affects PFKFB3

2 | 1 1

USP33 binds PFKFB3.

2 | 1

USP33 binds PFKFB3. 3 / 3

2 | 1

➶

sparser

"Collectively, these findings reveal a mechanism by which OS cells survive in a dystrophic tumor microenvironment, with the USP33-PFKFB3 axis as a critical driver of aerobic glycolysis and OS proliferation."

37034227

➶

| 2

USP33 activates Neoplasms. 2 / 2

| 2

➶

reach

"USP33/TGFBR2 axis promoted the tumor growth and metastasis of PC in vivo."

37322017

➶

reach

"Taking together, our results demonstrated that USP33 promoted the tumor growth and metastasis of PC in a TGFBR2-dependent manner."

37322017

USP33 affects MAPKAP1

| 4

USP33 binds MAPKAP1.

| 3

USP33 binds MAPKAP1. 3 / 3

| 3

➶

sparser

"As shown in Supplementary Fig. xref , only the full‐length (FL) and the M4 (717–942aa) and M5 (811–942aa) mutants of USP33, but not other mutants, interacted with SIN1."

40695806

➶

| 2

USP33 activates Glioma. 2 / 2

| 2

➶

reach

"Under hypoxic conditions, deubiquitinase USP33 promotes glioblastoma growth and maintains stemness of glioma stem cells by stabilizing hypoxia inducible factor-2α [12]."

38863009

➶

reach

"Silencing of USP33 disrupted glioma stem cells maintenance, reduced tumor vascularization, and inhibited glioblastoma growth."

35191554

USP33 affects EPAS1

2 | 1 1

USP33 binds EPAS1.

2 | 1

EPAS1 binds USP33. 3 / 3

2 | 1

➶

biogrid

No evidence text available

35191554

➶

sparser

"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha, leading to its stabilization through deubiquitination."

35191554

➶

biogrid

No evidence text available

35191554

USP33 deubiquitinates EPAS1.

1 |

➶

hprd

No evidence text available

11739384

USP33 affects E

2 | 2

E binds USP33. 4 / 4

2 | 2

➶

biogrid

No evidence text available

38569033

➶

biogrid

No evidence text available

| DOI

➶

sparser

"Moreover, we predicted more precise regions and sites of the interaction between E protein and USP33 T2 domain by AlphaFold 3."

39301916

➶

sparser

"The CO‐IP assay confirmed the existence of an interaction between USP33 and the E protein (Figure xref , Supporting Information)."

39301916

USP20 affects groEL2

| 4

GroEL2 binds USP33 and USP20. 4 / 4

| 4

➶

sparser

24874852

➶

sparser

"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

24874852

➶

sparser

"HSP56 interacts with both VDU1 and VDU2."

24874852

➶

sparser

24874852

TRAF3 affects USP33

| 1 3

USP33 binds TRAF3. 4 / 4

| 1 3

➶

sparser

"Protein interaction between USP33 and TRAF3 was identified by immunofluorescence staining."

39637362

➶

sparser

"Mechanistically, USP33 interacted with TRAF3 and induced TRAF3 deubiquitination to up-regulate its expression (P < 0.05)."

39637362

➶

sparser

"Moreover, USP33-TRAF3 activated the NF-κB pathway (P < 0.05)."

39637362

➶

reach

39637362

SP1 affects USP33

| 2 2

USP33 binds SP1. 4 / 4

| 2 2

➶

reach

"Mechanistically, USP33 directly bound SP1 and decreased its ubiquitination, thereby upregulating c-Met expression."

32835698

➶

sparser

"Intriguingly, the results indicated that endogenous USP33 and SP1 are directly bound in HCC cells ( Fig. 5 C and Supplemental Fig. 7A)."

32835698

➶

reach

37322017

➶

sparser

"To this end, we first performed Co-IP experiments to detect the interaction between USP33 and SP1 proteins in HCC cells."

32835698

JAK2 affects USP33

1 | 1 2

USP33 binds JAK2. 4 / 4

1 | 1 2

➶

sparser

37256445

➶

sparser

"Then, we further determined the interaction between USP33 and JAK2."

37256445

➶

reach

"Then, we further determined the interaction between USP33 and JAK2."

37256445

➶

biogrid

No evidence text available

37256445

HERC2 affects USP33

1 | 2 1

HERC2 binds USP33.

1 | 1 1

HERC2 binds USP33. 2 / 2

1 | 1

➶

reach

"13 HERC2 also interacts with the deubiquitinating enzyme USP33 and the SCF protein FBXL5, regulating their stability through ubiquitin mediated proteasomal degradation."

25476789

➶

biogrid

No evidence text available

31427565

HERC2 binds USP33 and FBXL5. 1 / 1

| 1

➶

sparser

"More recently, HERC2 was shown to interact with p53 through the p53 tetramerization domain, thus affecting p53 oligomerization and downstream transcriptional activity. xref HERC2 also interacts with the deubiquitinating enzyme USP33 and the SCF protein FBXL5, regulating their stability through ubiquitin-mediated proteasomal degradation. xref , xref HERC2 has also been shown to regulate centrosome morphology and ubiquitin ligase activity through interactions with NEURL4 and UBE3A (also known as E6AP), respectively."

25476789

HERC2 inhibits USP33.

| 1

HERC2 inhibits USP33. 1 / 1

| 1

➶

reach

"HERC2 knockdown increased levels of the USP33 protein and decreased levels of the p21 protein."

27528230

GCGR affects USP33

1 | 2 1

GCGR binds USP33.

1 | 1 1

GCGR binds USP33. 3 / 3

1 | 1 1

➶

sparser

"The USP33-GCGR complex likely dissembles at later times of agonist, as suggested by the decreasing interaction seen in the pulldown assay at 60 min of agonist stimulation ( xref , C and D )."

32967969

➶

reach

"The USP33-GCGR complex likely dissembles at later times of agonist, as suggested by the decreasing interaction seen in the pulldown assay at 60 min of agonist stimulation (Fig. 7, C and D)."

32967969

➶

biogrid

No evidence text available

32967969

GCGR activates USP33.

1 |

➶

hprd

No evidence text available

11739384

ELAVL1 affects USP33

sparser

"We demonstrated that hVDU1 could interact with the β-domain of pVHL and this interaction mediated the ubiquitination and degradation of VDU1 by pVHL E3 ligase [30] ."

12056827

E3_Ub_ligase increases the amount of USP33.

| 1

E3_Ub_ligase increases the amount of USP33. 1 / 1

| 1

➶

reach

"However, how cellular USP33 levels are regulated remains largely unexplored.Here we discovered that the E3 ligase beta-TrCP did not reduce USP33 mRNA levels, but significantly downregulated USP33 prot[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

28506875

E3_Ub_ligase decreases the amount of USP33.

| 1

E3_Ub_ligase decreases the amount of USP33. 1 / 1

| 1

➶

reach

28506875

E affects USP33

2 | 2

E binds USP33. 4 / 4

2 | 2

➶

biogrid

No evidence text available

38569033

➶

biogrid

No evidence text available

| DOI

➶

sparser

"Moreover, we predicted more precise regions and sites of the interaction between E protein and USP33 T2 domain by AlphaFold 3."

39301916

➶

sparser

"The CO‐IP assay confirmed the existence of an interaction between USP33 and the E protein (Figure xref , Supporting Information)."

39301916

Bisphenol A affects USP33

3 |

Bisphenol A increases the amount of USP33.

2 |

Bisphenol A increases the amount of USP33. 2 / 2

2 |

➶

ctd

No evidence text available

33296240

➶

ctd

No evidence text available

25181051

Bisphenol A decreases the amount of USP33.

| 3

USP33 increases the amount of α-SMA.

| 2

USP33 increases the amount of α-SMA. 2 / 2

| 2

➶

reach

"Conversely, the expression of α-SMA, COL1A2, and Hyp content were decreased by the USP33 knockdown, suggesting the hepatic stellate cell activation was downregulated by the USP33 knockdown."

37267862

➶

reach

"In addition, the protein levels of α-SMA, COL1A2, LDHA, and c -Myc were significantly reduced by Usp33 knockdown in primary hepatic stellate cells ( Fig. 3 F and G)."

37267862

USP33 activates α-SMA.

| 1

USP33 activates α-SMA. 1 / 1

| 1

➶

reach

"Moreover, the upregulation of Usp33, α-SMA, COL1A2, and LDHA induced by Usp33 were suppressed by the 10058-F4 ( Fig. 4 F)."

37267862

USP33 affects integrin α6

| 3

USP33 binds integrin α6.

| 2

USP33 binds integrin α6. 2 / 2

| 2

➶

reach

"Altogether, these results indicate that USP33 could bind and stabilize integrin α6 through deubiquitination."

39589547

➶

reach

39589547

USP33 activates integrin α6.

| 1

USP33 activates integrin α6. 1 / 1

| 1

➶

reach

39589547

USP33 affects centriole

| 3

USP33 activates centriole. 3 / 3

| 3

➶

eidos

"Moreover , knockdown of USP33 reverses centriole amplification in both NudCL2 KO and HERC2-depleted cells ."

31427565

➶

eidos

"Knockdown of USP33 suppressed centriole amplification in NudCL2 KO cells ( Fig. 6g-i ) ."

31427565

➶

eidos

"Moreover , downregulation of USP33 reverses centriole amplification induced by NudCL2 KO or HERC2 knockdown ( Figs ."

31427565

USP33 affects cell adhesion

| 3

USP33 activates cell adhesion. 3 / 3

| 3

➶

reach

"USP33 promotes laminin-dependent cell adhesion and migration."

39589547

➶

reach

"Consistent with the observations in these types of cancer, we found that USP33 promoted cell adhesion and migration under laminin-coating conditions, indicating candidate mediator proteins in laminin receptors are involved in USP33-mediate cell migratory ability."

39589547

➶

reach

"USP33 promoted laminin-dependent adhesion, spreading, and migration of ESCC cells but not their proliferation."

39589547

USP33 affects arrestins

| 3

USP33 deubiquitinates arrestins.

| 2

USP33 deubiquitinates arrestins. 1 / 1

| 1

➶

reach

"These results suggest that USP33 likely locally, rather than globally, deubiquitinates arrestins associated with D R/Mdm2.Knockdown of USP33 abolished the deubiquitination of arrestin3 (Fig. 4A), the desensitization of D R (Fig. 4B), and the nuclear entry of Gβγ and arrestin3 (Fig. 4C)."

| 3

USP33 inhibits USP33.

| 2

USP33 inhibits USP33. 2 / 2

| 2

➶

reach

"Furthermore, beta-TrCP also increased the ubiquitination of USP33 (^ DSG) and decreased the protein stability of USP33 (^ DSG)."

28506875

➶

reach

"The miR-148a carried within these EVs inhibits the expression level of the ubiquitin-specific peptidase 33 (USP33) protein."

38255898

USP33 increases the amount of USP33.

| 1

USP33 increases the amount of USP33. 1 / 1

| 1

➶

reach

37322017

USP33 affects TRAF6

2 | 1

USP33 binds TRAF6.

2 |

USP33 binds TRAF6. 2 / 2

2 |

➶

biogrid

No evidence text available

21525354

➶

biogrid

No evidence text available

21525354

USP33 deubiquitinates TRAF6.

| 3

USP33 inhibits SLIT2.

| 2

USP33 inhibits SLIT2. 2 / 2

| 2

➶

reach

"Enhanced USP33 expression increased Slit2 inhibition of cell migration (XREF_FIG), whereas knocking down USP33 blocked the inhibitory effect of Slit on cell migration (XREF_FIG)."

25242263

➶

reach

"Ectopic expression of Slit2 significantly reduced the number of lung metastasis sites, and USP33 overexpression enhanced the inhibitory effect of Slit2 on tumor metastasis (XREF_FIG, XREF_SUPPLEMENTARY)."

25242263

USP33 increases the amount of SLIT2.

sparser

23486064

USP33 binds NEURL4. 1 / 1

1 |

➶

biogrid

No evidence text available

23486064

USP33 affects MET

| 2 1

USP33 activates MET.

| 2

USP33 activates MET. 2 / 2

| 2

➶

eidos

"We found that USP33 knockdown inhibited the invasion and metastasis in HCC cells both in vitro and in vivo , which was partly dependent on c-Met ."

32835698

➶

eidos

"Mechanistically , USP33 directly bound SP1 and decreased its ubiquitination , thereby upregulating c-Met expression ."

32835698

USP33 binds MET.

| 1

USP33 binds MET. 1 / 1

| 1

➶

sparser

"Unexpectedly, the direct interaction between USP33 and c-Met proteins was not observed in HCC cells by coimmunoprecipitation (Co-IP) experiments ( Fig. 4 A and Supplemental Fig. 5A)."

32835698

USP33 affects LDHA

| 3

USP33 increases the amount of LDHA.

| 2

USP33 increases the amount of LDHA. 2 / 2

| 2

➶

reach

"In addition, the protein levels of α-SMA, COL1A2, LDHA, and c -Myc were significantly reduced by Usp33 knockdown in primary hepatic stellate cells ( Fig. 3 F and G)."

37267862

reach

"13 HERC2 also interacts with the deubiquitinating enzyme USP33 and the SCF protein FBXL5, regulating their stability through ubiquitin mediated proteasomal degradation."

25476789

➶

biogrid

No evidence text available

31427565

HERC2 binds USP33 and FBXL5. 1 / 1

| 1

➶

sparser

25476789

USP33 affects FLVCR1

| 3

USP33 activates FLVCR1. 3 / 3

| 3

➶

reach

"Thus, USP33 promotes PCa cell proliferation and self-renewal by inhibiting the degradation of CTNNB1."

37076992

➶

reach

"Herein, we report that USP33 silencing inhibits PCa cell survival and self-renewal."

37076992

➶

reach

"USP33 promotes the development of PCa through two pathways."

36811009

USP33 affects Cell Survival

| 1 2

USP33 activates Cell Survival.

| 1 1

USP33 activates Cell Survival. 2 / 2

| 1 1

➶

reach

"Herein, we report that USP33 silencing inhibits PCa cell survival and self-renewal."

37076992

➶

eidos

"USP33 enhances cell survival and stemness by deubiquitinating CTNNB1 in BXPC-3 and SW1990 cells ."

37076992

USP33 inhibits Cell Survival.

| 1

USP33 inhibits Cell Survival. 1 / 1

| 1

➶

reach

"Knockdown of USP33 increased cell viability and inhibited cellular pyroptosis, accompanied by decreases in IL-1β, IL-18, and Caspase-1."

39506557

USP33 affects CRC

| 3

CRC binds ROBO1 and USP33. 2 / 2

| 2

➶

sparser

"In CRC cells, USP33 interacts with Robo1 and mediates the inhibitory activity of Slit2 on CRC cell migration by deubiquitinating and stabilizing Robo1."

25242263

➶

sparser

"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

2 1 |

USP33 binds ARRB1.

2 |

USP33 binds ARRB1. 2 / 2

2 |

➶

biogrid

No evidence text available

19363159

➶

biogrid

No evidence text available

19363159

USP33 deubiquitinates ARRB1.

sparser

"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

25242263

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

| 1

➶

sparser

"These results confirmed that Robo1 could interact with srGAP1 and USP33 in CRC cells, and srGAP1 could not interact with USP33 directly."

27923383

PFKFB3 affects USP33

2 | 1

USP33 binds PFKFB3. 3 / 3

2 | 1

➶

sparser

37034227

➶

biogrid

No evidence text available

37034227

➶

biogrid

No evidence text available

37034227

NEURL4 affects USP33

1 | 2

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

| 2

➶

sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

➶

sparser

23486064

USP33 binds NEURL4. 1 / 1

1 |

➶

biogrid

No evidence text available

23486064

MAPKAP1 affects USP33

| 3

USP33 binds MAPKAP1. 3 / 3

| 3

➶

sparser

"As shown in Supplementary Fig. xref , only the full‐length (FL) and the M4 (717–942aa) and M5 (811–942aa) mutants of USP33, but not other mutants, interacted with SIN1."

40695806

➶

sparser

40695806

➶

sparser

"Third, CDK1-mediated phosphorylation was essential for USP33 binding to SIN1 and promoting tumor progression (Fig. xref )."

40695806

Hypoxia affects USP33

| 1 2

Hypoxia activates USP33. 3 / 3

| 1 2

➶

reach

"ELAVL1 decrease repressed the proliferation and microtubule formation of hypoxia-induced HRECs by reducing USP33."

39320536

➶

eidos

"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha , leading to its stabilization through deubiquitination ."

35191554

➶

reach

"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha, leading to its stabilization through deubiquitination."

35191554

EPAS1 affects USP33

2 | 1

EPAS1 binds USP33. 3 / 3

2 | 1

➶

biogrid

No evidence text available

35191554

➶

sparser

"USP33 is preferentially induced in glioma stem cells by hypoxia and interacts with HIF-2alpha, leading to its stabilization through deubiquitination."

35191554

➶

biogrid

No evidence text available

35191554

CRC affects USP33

| 3

CRC binds ROBO1 and USP33. 2 / 2

| 2

➶

sparser

"In CRC cells, USP33 interacts with Robo1 and mediates the inhibitory activity of Slit2 on CRC cell migration by deubiquitinating and stabilizing Robo1."

25242263

➶

sparser

"To test the interaction between Robo1 and USP33 in CRC cells, we performed co-IP assays."

reach

"Usp33 interacts with beta 2 AR but mechanistically how this occurs is not known."

23201781

AR binds ARRB2 and USP33. 1 / 1

| 1

➶

sparser

23764054

7b affects USP33

2 | 1

7b binds USP33. 3 / 3

2 | 1

➶

biogrid

No evidence text available

2 |

➶

biogrid

No evidence text available

28514442

➶

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

| 2

| 2

➶

reach

"USP33 can also inhibit tumor progression of colorectal cancer by targeting Slit-Robo signaling pathway or beta-arrestin signaling [XREF_BIBR, XREF_BIBR]."

29574466

➶

reach

28064438

USP33 affects ZNRF4

2 |

ZNRF4 binds USP33. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

2 |

➶

biogrid

No evidence text available

28514442

➶

2 |

➶

biogrid

No evidence text available

29576527

➶

biogrid

No evidence text available

24377933

USP33 affects TNF

| 2

USP33 inhibits TNF.

| 1

USP33 inhibits TNF. 1 / 1

| 1

➶

reach

"Reduced USP33 has been shown to decrease the stability of a suppressor molecule of major proinflammatory gene expression pathways of TNF-α, NF-κB, and IFN-β (22)."

35046947

USP33 increases the amount of TNF.

reach

"Two T. brucei orthologues of human deubiquitinases Usp7 and Vdu1 designated TbUsp7 and TbVdu1 were confirmed to be expressed in the parasite, and their knockdown increased the turnover of ISG75 and ISG65 (Zoltner et al., 2015)."

37088149

➶

| 2

➶

sparser

"In marked contrast, HA-VDU1 interacted strongly with hSP56/Se ( Fig. 2 C)."

19118533

➶

sparser

"No significant interaction between HA-VDU1 and BSA or hSP56 was observed."

19118533

USP33 affects Glioblastoma

| 2

USP33 activates Glioblastoma. 2 / 2

| 2

➶

reach

"Under hypoxic conditions, deubiquitinase USP33 promotes glioblastoma growth and maintains stemness of glioma stem cells by stabilizing hypoxia inducible factor-2α [12]."

38863009

1 |

➶

No evidence text available

33961781

USP33 affects AKT1

2 |

AKT1 binds USP33. 2 / 2

2 |

➶

biogrid

No evidence text available

36658650

➶

biogrid

No evidence text available

36658650

USP33 affects (S)-lactic acid

| 2

USP33 increases the amount of (S)-lactic acid. 2 / 2

| 2

➶

reach

37267862

➶

reach

37267862

USP20 affects NEURL4

| 2

| 2

➶

sparser

"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

23486064

➶

sparser

23486064

LPCAT4 affects USP33

2 |

LPCAT4 binds USP33. 2 / 2

2 |

➶

biogrid

No evidence text available

33961781

➶

| 2

➶

sparser

"In marked contrast, HA-VDU1 interacted strongly with hSP56/Se ( Fig. 2 C)."

19118533

➶

sparser

"No significant interaction between HA-VDU1 and BSA or hSP56 was observed."

19118533

Gentamicins affects USP33

2 |

Gentamicins increases the amount of USP33. 2 / 2

2 |

➶

ctd

No evidence text available

33387578

➶

ctd

No evidence text available

22061828

ELOB affects USP33

1 1 |

ELOB binds USP33. 1 / 1

1 |

➶

| 2

➶

sparser

"We also tested a catalytically inactive USP33 in which the cysteine and histidine residues of the active site are mutated (USP33-CH) for its effect on GCGR ubiquitination."

32967969

➶

sparser

32967969

CCP110 affects USP20

| 2

reach

38874156

USP33 affects USP8

| 1

USP33 inhibits USP8. 1 / 1

| 1

➶

reach

37322017

USP33 affects SMAD

| 1

| 1

➶

sparser

21476968

USP33 affects 3a

1 |

3a binds USP33. 1 / 1

1 |

➶

biogrid

No evidence text available

37478010

USP29 affects USP33

| 1

➶

reach

37322017

TGFBR1 affects USP33

1 |

USP33 binds TGFBR1. 1 / 1

1 |

➶

biogrid

No evidence text available

37322017

TGFB affects USP33

| 1

➶

reach

37322017

SLIT3 affects USP33

| 1

reach

30528740

HERC2 affects FBXL5

| 1

| 1

➶

sparser

33922750

DUSP1 affects JNK, and USP33

| 1

JNK binds USP33 and DUSP1. 1 / 1

| 1

➶

sparser

"Furthermore, we found that USP33 could interact with the phosphatase DUSP1 to negatively regulate the activation of JNK, while USP33 knockdown promoted the proteasomal degradation of DUSP1."

31857702

DUSP1 domain affects USP33

| 1

| 1

➶

reach

12865408

(+)-JQ1 compound affects USP33

1 |

(+)-JQ1 compound decreases the amount of USP33. 1 / 1

1 |

➶

ctd

No evidence text available

23086925

4,4'-sulfonyldiphenol affects USP33

1 |

4,4'-sulfonyldiphenol decreases the amount of USP33. 1 / 1

1 |

➶

ctd

No evidence text available

36041667

IndraLab

Statements

USP33 affects ROBO1

USP33 binds ROBO1.

ROBO1 binds USP33. 10 / 34

CRC binds ROBO1 and USP33. 2 / 2

ROBO1 binds CRC, SRGAP1, and USP33. 1 / 1

ROBO1 binds HTATIP2 and USP33. 1 / 1

USP33 deubiquitinates ROBO1.

USP33 deubiquitinates ROBO1. 9 / 9

Modified USP33 leads to the deubiquitination of ROBO1. 1 / 1

USP33 deubiquitinates ROBO1 on lysine. 1 / 1

USP33 increases the amount of ROBO1.

USP33 increases the amount of ROBO1. 2 / 2

Modified USP33 increases the amount of ubiquitinated ROBO1. 1 / 1

Modified USP33 increases the amount of ROBO1. 1 / 1

USP33 inhibits ROBO1.

USP33 inhibits ROBO1. 2 / 3

USP33 decreases the amount of ROBO1.

USP33 decreases the amount of ubiquitinated ROBO1. 2 / 2

USP33 decreases the amount of ROBO1. 1 / 1

USP33 activates ROBO1.

USP33 activates ROBO1. 1 / 2

USP33 ubiquitinates ROBO1.

USP33 leads to the ubiquitination of ROBO1. 1 / 1

USP33 affects ARRB2

USP33 binds ARRB2.

ARRB2 binds USP33. 10 / 23

AR binds ARRB2 and USP33. 1 / 1

USP33 deubiquitinates ARRB2.

USP33 deubiquitinates ARRB2. 7 / 7

USP33 ubiquitinates ARRB2.

USP33 leads to the ubiquitination of ARRB2. 2 / 2

USP33 decreases the amount of ARRB2.

USP33 decreases the amount of ARRB2. 2 / 2

USP33 increases the amount of ARRB2.

USP33 increases the amount of ARRB2. 1 / 1

USP33 affects CCP110

USP33 binds CCP110.

USP33 binds CCP110. 9 / 13

USP20 binds USP33, NEURL4, and CCP110. 2 / 2

USP33 activates CCP110.

USP33 activates CCP110. 4 / 8

USP33 deubiquitinates CCP110.

USP33 deubiquitinates CCP110. 5 / 5

USP33 inhibits CCP110.

USP33 inhibits CCP110. 1 / 3

USP33 ubiquitinates CCP110.

USP33 ubiquitinates CCP110. 1 / 1

USP33 affects cell population proliferation

USP33 activates cell population proliferation.

USP33 activates cell population proliferation. 10 / 18

USP33 inhibits cell population proliferation.

USP33 inhibits cell population proliferation. 10 / 12

BTRC affects USP33

BTRC binds USP33.

BTRC binds USP33. 10 / 13

BTRC activates USP33.

BTRC activates USP33. 8 / 8

BTRC ubiquitinates USP33.

BTRC leads to the ubiquitination of USP33. 2 / 2

Modified BTRC leads to the ubiquitination of USP33. 1 / 1

BTRC decreases the amount of USP33.

BTRC decreases the amount of USP33. 2 / 3

BTRC inhibits USP33.

BTRC inhibits USP33. 2 / 2

USP33 affects Neoplasm Invasiveness

USP33 activates Neoplasm Invasiveness.

USP33 activates Neoplasm Invasiveness. 10 / 19

USP33 inhibits Neoplasm Invasiveness.

USP33 inhibits Neoplasm Invasiveness. 9 / 9

VHL affects USP33

VHL binds USP33.

VHL binds USP33. 10 / 19

VHL binds USP33 and USP20. 1 / 1

ELOC binds USP33, ELOB, and VHL. 1 / 1

VHL ubiquitinates USP33.

VHL bound to RBX1, CUL2, ELOC, and ELOB ubiquitinates USP33. 2 / 2

VHL ubiquitinates USP33. 2 / 2

VHL decreases the amount of USP33.