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CDK1 phosphorylates USP33. 6 / 6
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"First, we demonstrate that CDK1 binds and phosphorylates USP33 at Ser650 and Ser843, and the phosphorylation-deficient 2A mutation of USP33 failed to undergo phosphorylation (Fig. xref and Supplementary Fig. xref )."
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"As shown in Fig. xref , active CDK1 phosphorylated GST-fused USP33 WT in vitro, while the 2A mutant exhibited significantly reduced phosphorylation."
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"CDK1 binds and phosphorylates USP33 at Ser650 and Ser843."
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"We next explored whether CDK1 may phosphorylate USP33 and affect its tumor-promoting function in PDAC cells."
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"Additionally, we show that CDK1 directly phosphorylates USP33, enhancing its deubiquitinase activity toward SIN1 and driving PDAC progression."
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"To further confirm that CDK1 directly phosphorylates USP33, we performed an in vitro kinase assay, where GST-fused USP33 WT or the 2A mutant was incubated with active CDK1."
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