IndraLab

Statements

DUSP2 binds USP33. 5 / 5
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"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"We were successful in producing the USP4-DUSP, USP11-DUSP, USP15-DUSP, USP20-DUSP2 and USP33-DUSP2 recombinant proteins in Escherichia coli and purified them to near homogeneity from the soluble fract[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Under native conditions, size-exclusion chromatography (SEC) demonstrated that each DUSP protein existed as a stable monomer, except USP33-DUSP2, which eluted as two peaks with one peak corresponding [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Finally, among six UbVs selected for binding to USP33-DUSP2, three exhibited some non-specific binding to SAV (33D2.4–6) but the other three exhibited absolute specificity for their cognate target (33[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"For USP15-DUSP and USP33-DUSP2, the best UbVs exhibited affinities in the low nanomolar range (K D ∼ 10 nM), whereas for USP11-DUSP and USP20-DUSP2, affinities were in the mid-nanomolar range (K D ∼ 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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