IndraLab

Statements


USP20 is modified
42 | 2 47 14
USP20 is phosphorylated.
42 | 36 14
USP20 is phosphorylated. 10 / 37
| 23 14

rlimsp
"As shown in Figure 5D and Supplementary Figure S4C, ATR depletion compromised USP20 phosphorylation at the SQ/TQ motifs and resulted in USP20 downregulation, suggesting that ATR is the major kinase responsible for USP20 phosphorylation and upregulation following replication stress."

sparser
"Upon replication stress, ATR-mediated phosphorylation of USP20 promotes dissociation of HERC2 from USP20, stabilizes USP20 and its association with CLASPIN, thus increasing CALSPIN stability and ensuring CHK1 activation."

sparser
"USP20 phosphorylation by ATR does not affect its E3 ligase activity per se , suggesting that USP20 levels, but not catalytic activity, are subjected to ATR regulation."

sparser
"As shown in Figure xref and Supplementary Figure S4C, ATR depletion compromised USP20 phosphorylation at the SQ/TQ motifs and resulted in USP20 downregulation, suggesting that ATR is the major kinase responsible for USP20 phosphorylation and upregulation following replication stress."

sparser
"Similar to other DNA repair proteins, USP20 is phosphorylated post DNA damage, and its depletion sensitizes cancer cells to damaging agents that form blocks ahead of the replication forks."

rlimsp
"We found that individual mutation at these sites partially affected USP20 phosphorylation, while mutating all four sites (4 mut) abolished USP20 phosphorylation (Figure 5E and F)."

sparser
"This occurred in a phosphorylation-dependent manner, because only the non-phosphorylated USP20 was active."

sparser
"These suggest that ATR-mediated phosphorylation of USP20 promotes dissociation of USP20 from HERC2, and this dissociation would stabilize CLASPIN and promote CHK1 activation."

sparser
"Under replication stress, ATR-mediated phosphorylation of USP20 results in the disassociation of HERC2 from USP20."

rlimsp
"(D) USP20 was phosphorylated on its S/TQ motifs. 293T cells were transfected with FLAG-VEC, FLAG-USP20 or FLAG-USP20(4SA), in which all the four S/TQ sites within the USP20 polypeptide were mutated to AQ."
USP20 is phosphorylated on S132. 10 / 11
10 | 1

No evidence text available

sparser
"The post-prandial increase in insulin and glucose concentration stimulates mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is recruited to the HMGCR complex and antagonizes its degradation."

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USP20 is phosphorylated on S134. 10 / 11
10 | 1

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USP20 is phosphorylated on S333. 10 / 11
| 11

sparser
"To determine whether USP20 Ser334 phosphorylation regulates pro-inflammatory signaling, we created USP20-S334A mice using CRISPR/Cas9-mediated gene editing."

sparser
"Reversible phosphorylation of USP20 at S333 could be a critical factor that determines the rate of down-regulation of β 2 ARs via autophagosomes during catecholamine stress [ xref ]."

sparser
"In this process, phosphorylation of USP20 at serine residue 333 is required for its activity providing an additional level of regulation [148]."

sparser
"The association of USP20 with its substrates triggers deubiquitinase activity; this association is regulated by phosphorylation of USP20 on Ser334 (mouse) or Ser333 (human)."

sparser
"In this process, phosphorylation of USP20 at serine residue 333 is required for its activity providing an additional level of regulation [ xref ]."

sparser
"Recent studies have shown that β 2 AR activation triggers site-specific phosphorylation of USP20 by the cAMP-activated protein kinase A (PKA) at serine 333 of USP20 [ xref ]."

sparser
"Our findings reveal novel mechanisms regulating IL-1β-induced pro-inflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation and neointimal hyperplasia."

sparser
"The association of USP20 with its substrates triggers deubiquitinase activity; this association is regulated by phosphorylation of USP20 on Ser334 (mouse) or Ser333 (human)."

sparser
"USP20 Ser333 phosphorylation was greater in SMCs of atherosclerotic segments of human arteries as compared with non-atherosclerotic segments."

sparser
"WT carotid SMCs showed substantial phosphorylation of USP20 Ser334, and WT carotids demonstrated greater NFκB activation, VCAM-1 expression, and SMC proliferation than USP20-S334A carotids."
USP20 is phosphorylated on S373. 4 / 4
4 |

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USP20 is phosphorylated on S413. 4 / 4
4 |

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USP20 is phosphorylated on S407. 4 / 4
4 |

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USP20 is phosphorylated on T377. 2 / 2
2 |

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USP20 is phosphorylated on T258. 2 / 2
2 |

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USP20 is phosphorylated on S372. 2 / 2
2 |

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USP20 is phosphorylated on S406. 2 / 2
2 |

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USP20 is phosphorylated on S263. 2 / 2
2 |

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USP20 is ubiquitinated.
| 2 10
USP20 is ubiquitinated. 10 / 12
| 2 10

sparser
"Additionally, USP20 could be ubiquitinated in a pVHL-dependent manner, inducing itself rapid degradation ( xref )."

sparser
"Data from the literature indicate that pVHL30 mediates the ubiquitination and degradation of both USP33 and USP20, in turn down-regulating the pathways controlled by these two proteins [ xref ]."

sparser
"Although pVHL functions as a master control for HIF-1alpha stabilization, as pVHL-E3 ligase mediates the ubiquitination of both HIF-1alpha and VDU2, the balance between the pVHL-mediated ubiquitination and VDU2-mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."

reach
"The balance between the VHL mediated ubiquitination and VDU2 mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."

sparser
"The HECT domain of WT HERC but not the catalytic inactive mutant (CA) ubiquitinated USP20 in vitro (Figure xref )."

sparser
"Finally, we demonstrate that VDU2 can also be ubiquitinated and degraded in a pVHL-dependent manner."

sparser
"As USP20 ubiquitination decreases following replication stress, we next studied whether the USP20-HERC2 interaction is subjected to regulation by the DDR pathway."

sparser
"We found that USP20 was ubiquitinated in unstressed cells and USP20 ubiquitination was dramatically decreased after UV or HU treatment (Figure xref ), suggesting that USP20 is regulated by the ubiquitin-proteasome pathway."

sparser
"On the other hand, when cells recover from DNA damage, HERC2 mediates USP20 ubiquitination, turning off the DDR signaling."

sparser
"Downregulation of HERC2 resulted in significant decrease in USP20 ubiquitination in vivo (Figure xref )."
USP20 is dephosphorylated.
| 1
USP20 is dephosphorylated. 1 / 1
| 1

sparser
"Only dephosphorylated USP20 shows robust activity for deubiquitinating the β 2 AR."
USP20 affects CLSPN
7 1 | 27 5
USP20 binds CLSPN.
7 | 4 5
7 | 4 4

reach
"To determine whether the interaction between USP20 and Claspin is direct, we generated and purified recombinant USP20 and Claspin."

reach
"However, depletion of HERC2 did not affect the binding between USP20 and Claspin."

No evidence text available

sparser
"We confirmed the USP20-Claspin interaction by Co-IP."

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reach
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex and both USP20 and CLASPIN were in the HERC2 immunocomplex, suggesting that HERC2, USP20 and CLASPIN form a complex."

No evidence text available

reach
"As shown in Figure XREF_FIG, GST-USP20 was able to interact with His Claspin under cell-free conditions, suggesting a direct interaction between USP20 and Claspin."

sparser
"To determine whether the interaction between USP20 and Claspin is direct, we generated and purified recombinant USP20 and Claspin."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
USP20 deubiquitinates CLSPN.
1 | 12
USP20 deubiquitinates CLSPN. 10 / 11
1 | 10

reach
"USP20 deubiquitinates and stabilizes Claspin."

reach
"USP20 deubiquitinates and stabilizes Claspin, which in turn facilitate the activation of cell-cycle checkpoint following DNA damage."

reach
"To determine whether USP20 directly deubiquitinates Claspin, we performed an in vitro deubiquitination assay."

reach
"CLASPIN was also deubiquitinated by bacterially produced GST-USP20, but not by the catalytically inactive mutant GST USP20 (C154S), in the in vitro deubiquitination assays."

reach
"These results revealed that USP20 mediated Claspin downregulation did not occur at the gene transcription level, just as the prior study reported that USP20 deubiquitinate and stabilize Claspin."

reach
"USP20 in turn deubiquitinates and stabilizes Claspin and enhances the activation of ATR-Chk1 signaling."

reach
"Next, we tested whether USP20 deubiquitinates Claspin."

reach
"These results suggest that USP20 deubiquitinates Claspin in cells."

reach
"Unbound USP20 is enzymatically active and mediates Claspin deubiquitination and stabilization, this way promoting Chk1 activation [49,50]."

"USP20 in turn deubiquitinates and stabilizes Claspin and enhances the activation of ATR-Chk1 signaling."
USP20-C154S deubiquitinates CLSPN. 1 / 1
| 1

reach
"CLASPIN was also deubiquitinated by bacterially produced GST-USP20, but not by the catalytically inactive mutant GST USP20 (C154S), in the in vitro deubiquitination assays."
USP20 deubiquitinates ubiquitinated CLSPN. 1 / 1
| 1

reach
"USP20 in turn deubiquitinates K48-linked-polyubiquitinated CLASPIN, stabilizing CLASPIN and ultimately promoting CHK1 phosphorylation and CHK1 directed checkpoint activation."
USP20 activates CLSPN.
| 3
USP20 activates CLSPN. 3 / 9
| 3

reach
"USP20 promotes CLASPIN stability through deubiquitination."

reach
"Given that HERC2 is a component of the replication complex, our data shown in this report suggest that HERC2 and USP20 coordinately modulate CLASPIN stability during S-phase and in response to replication stress."

reach
"These results revealed that USP20 mediated Claspin downregulation did not occur at the gene transcription level, just as the prior study reported that USP20 deubiquitinate and stabilize Claspin."
USP20 increases the amount of CLSPN.
| 3
USP20 increases the amount of CLSPN. 1 / 2
| 1

reach
"Furthermore, reconstitution of WT USP20 but not catalytically inactive mutant of USP20 (USP20CA) in USP20 depleted cells restored Claspin protein levels."
Modified USP20 increases the amount of CLSPN. 1 / 1
| 1

reach
"As shown in XREF_FIG, overexpression of USP20 increased the protein level of Claspin without the change of mRNA level."
Mutated USP20 increases the amount of CLSPN. 1 / 1
| 1

reach
"Furthermore, reconstitution of WT USP20 but not catalytically inactive mutant of USP20 (USP20CA) in USP20 depleted cells restored Claspin protein levels."
USP20 ubiquitinates CLSPN.
| 2
USP20 ubiquitinates CLSPN. 2 / 2
| 2

reach
"Conversely, knocking-down USP20 increased polyubiquitination of Claspin."

reach
"As shown in Figure XREF_FIG, overexpressing USP20 resulted in a significant decrease in polyubiquitination of Claspin, while overexpressing USP20CA mutant had no such effect."
USP20 inhibits CLSPN.
| 2
USP20-C154S inhibits CLSPN. 1 / 1
| 1

reach
"Concomitantly, expression of the catalytically inactive mutant FLAG USP20 (C154S) reduced the half-life of CLASPIN."
USP20 inhibits CLSPN. 1 / 1
| 1

reach
"Furthermore, downregulation of USP20 blocked UV induced Claspin upregulation."
USP20 decreases the amount of CLSPN.
| 1
USP20 decreases the amount of CLSPN. 1 / 1
| 1

reach
"Prior studies have demonstrated that USP20 regulate the cell proliferation through the ATK-Claspin-CHK signaling pathway, and downregulation of USP20, could reduce the expression of Claspin through the ubiquitin-proteasome degradation and promote the DNA synthesis, suggesting a defect in the intra-S-phase checkpoint."
HERC2 affects USP20
7 1 | 20 16
HERC2 binds USP20.
7 | 12 14
7 | 12 13

reach
"As shown in Figure XREF_FIG, the interaction between HERC2 and USP20 decreased at early time point following DNA damage and then increased at later time point."

reach
"In unstressed cells, HERC2 binds USP20 to maintain a low basal level of USP20 in cells."

reach
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex and both USP20 and CLASPIN were in the HERC2 immunocomplex, suggesting that HERC2, USP20 and CLASPIN form a complex."

sparser
"We found that treating cells with the pan PIKK inhibitor caffeine blocked the disassociation of USP20 and HERC2 after UV treatment (Figure xref ), suggesting that PIKKs might be involved in the regulation of USP20-HERC2 interaction."

No evidence text available

reach
"We found that the presence of USP20 in the HERC2 immunocomplex decreased upon HU treatment, so did the presence of HERC2 in the FALG-USP20 immunocomplex, whereas the interaction between USP20 (4SA) and HERC2 did not decrease in response to HU treatment."

sparser
"We next studied how HERC2-USP20 interaction is regulated by the DDR pathway."

reach
"We confirmed the interaction between USP20 and HERC2 by endogenous Co-IP."

reach
"One is that USP20 phosphorylation itself affects the binding between USP20 and HERC2."

sparser
"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
HERC2 ubiquitinates USP20.
1 | 3 2
HERC2 ubiquitinates USP20. 6 / 7
1 | 3 2

sparser
"To determine whether HERC2 directly ubiquitinates USP20, we performed an in vitro ubiquitination assay."

reach
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

reach
"To determine whether HERC2 directly ubiquitinates USP20, we performed an in vitro ubiquitination assay."

sparser
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination-mediated proteasomal degradation of USP20, regulating the status of K48-linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

reach
"On the other hand, when cells recover from DNA damage, HERC2 mediates USP20 ubiquitination, turning off the DDR signaling."
HERC2 inhibits USP20.
| 4
HERC2 inhibits USP20. 4 / 4
| 4

reach
"Here we found that HERC2 antagonized USP20 function and negatively regulated the replication stress signaling pathway."

reach
"In HERC2 depleted cells, USP20 and its substrates Claspin were significantly upregulated and the phosphorylation of CHK1 is more sustained, even at later time points."

reach
"Taken together, these results suggested that HERC2 functions as an E3 ligase of USP20 and negatively regulates USP20 in unstressed cells."

reach
"HERC2 promotes USP20 degradation."
HERC2 increases the amount of USP20.
| 1
HERC2 increases the amount of USP20. 1 / 1
| 1

reach
"As shown in Figure XREF_FIG and XREF_FIG, downregulation of HERC2 resulted in upregulation of USP20 levels and protein stability."
USP20 affects USP33
2 | 5 15
USP20 binds USP33.
2 | 4 15
2 | 4 4

No evidence text available

reach
"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Moreover, pVHL30 is also known to interact with both USP33 and USP20, two enzymes belonging to the USP protein family, whose USP7 is a further member [ xref ]."

sparser
"Two DUBs, USP33 (or VDU-1) and USP20 (or VDU-2), interact with and regulate D2 [ xref ]."

No evidence text available

reach
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays (XREF_SUPPLEMENTARY) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 (XREF_SUPPLEMENTARY)."

sparser
"Like VDU1, VDU2 interacts with pVHL beta-domain and these two proteins can compete with each other to bind to pVHL."

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In the current studies and together with our previous report [30] , we have demonstrated that both VDU1 and VDU2 also bind to the β-domain of pVHL and several naturally occurring mutations in the β-d[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
USP20 binds VHL and USP33. 2 / 2
| 2

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

sparser
"Based on their amino acid sequence homology and functional interaction with pVHL, VDU1 and VDU2 define a subfamily of ubiquitin specific processing proteases."
USP20 binds USP33 and AR. 1 / 1
| 1

sparser
"Because carvedilol enhanced β 2 AR ubiquitination and degradation independently of the known β 2 AR E3 ligase Nedd4, we reasoned that carvedilol might diminish β 2 AR deubiquitination by reducing the association of the β 2 AR with USP20 and/or USP33."
| 1

sparser
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
USP20 binds USP33 and ARs. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP20 activates USP33.
| 1
USP20 activates USP33. 1 / 1
| 1

reach
"The closely related DUBs, USP20 and USP33, deubiquitylate activated beta-adrenergic receptors as well as the adaptor and signalling protein beta-arrestin (in the case of USP33), to favour recycling from endosomes, despite largely being associated with the secretory pathway 75, 76, 77."
ATR affects USP20
1 | 9 9 3
ATR phosphorylates USP20.
| 8 9 3
ATR phosphorylates USP20. 10 / 21
| 8 9 3

rlimsp
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU-induced replication stress."

rlimsp
"ATR-mediated phosphorylation of USP20 promotes its dissociation from HERC2."

reach
"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."

sparser
"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."

sparser
"Upon DNA damage, ATR phosphorylates USP20, a deubiquitinating enzyme which is a degradation target of HERC2 ( xref A)."

sparser
"Besides, ATR phosphorylates and also stabilizes USP20 by unbinding it from HERC2."

reach
"USP20 phosphorylation by ATR does not affect its E3 ligase activity per se, suggesting that USP20 levels, but not catalytic activity, are subjected to ATR regulation."

rlimsp
"(B and C) USP20 was phosphorylated by ATR in response to HU treatment."

reach
"Mechanistically, the phosphorylation of USP20 by ATR disrupts the interaction between HERC2 and USP20 and is responsible for USP20 upregulation following the DDR."

sparser
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU-induced replication stress."
ATR binds USP20.
1 |
1 |

No evidence text available
ATR activates USP20.
| 1
ATR activates USP20. 1 / 1
| 1

reach
"Since USP20 is upregulated after replication stress, we sought to determine whether USP20 is modulated by ATR in response to replication stress."
VHL affects USP20
4 2 | 11 5
VHL binds USP20.
4 | 7 3
4 | 7 1

No evidence text available

reach
"This mutation did not affect the interaction between hVDU2 and pVHL as predicted.VDU1 and VDU2 exhibit a very high homology at their N-terminal and C-terminal region as shown in Fig. 1, and both prote[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."

reach
"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The interaction between hVDU2 and pVHL was also tested by in vivo co-immunoprecipitation."

reach
"PVHL was precipitated by an anti-GFP antibody due to the interaction between hVDU2 and pVHL."

No evidence text available

reach
"Our data have demonstrated that several naturally occurring mutations in the pVHL beta-domain disrupt the interaction between hVDU2 and pVHL, which underscores the functional relevance of this interac[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 binds VHL and USP33. 2 / 2
| 2

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

sparser
"Based on their amino acid sequence homology and functional interaction with pVHL, VDU1 and VDU2 define a subfamily of ubiquitin specific processing proteases."
VHL ubiquitinates USP20.
2 | 4 2
VHL ubiquitinates USP20. 8 / 8
2 | 4 2

reach
"To test whether hVDU2 can be ubiquitinated by pVHL E3 ubiquitin ligase complex, we performed ubiquitination assay in vitro and in vivo."

reach
"These results suggest that like hVDU1, the ubiquitination of hVDU2 is dependent on the presence of pVHL.To test whether hVDU2 ubiquitination mediated by pVHL could result in rapid turnover of hVDU2 pr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"In this report we show that another uncharacterized deubiquitinating enzyme, named VDU2 (pVHL-interacting deubiquitinating enzyme-2), is a substrate of pVHL"

reach
"Moreover, VHL can ubiquitinate USP20 in return and target it for degradation [XREF_BIBR]."

sparser
"Moreover, VHL can ubiquitinate USP20 in return and target it for degradation [78]."

"Although pVHL functions as a master control for HIF-1alpha stabilization, as pVHL-E3 ligase mediates the ubiquitination of both HIF-1alpha and VDU2, the balance between the pVHL-mediated ubiquitination and VDU2-mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization"

sparser
"Moreover, VHL can ubiquitinate USP20 in return and target it for degradation [ xref ]."

reach
"Although pVHL functions as a master control for HIF-1alpha stabilization, as pVHL-E3 ligase mediates the ubiquitination of both HIF-1alpha and VDU2, the balance between the pVHL mediated ubiquitination and VDU2 mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."
USP33 affects USP20
2 | 4 15
2 | 4 4

No evidence text available

reach
"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Moreover, pVHL30 is also known to interact with both USP33 and USP20, two enzymes belonging to the USP protein family, whose USP7 is a further member [ xref ]."

sparser
"Two DUBs, USP33 (or VDU-1) and USP20 (or VDU-2), interact with and regulate D2 [ xref ]."

No evidence text available

reach
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays (XREF_SUPPLEMENTARY) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 (XREF_SUPPLEMENTARY)."

sparser
"Like VDU1, VDU2 interacts with pVHL beta-domain and these two proteins can compete with each other to bind to pVHL."

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In the current studies and together with our previous report [30] , we have demonstrated that both VDU1 and VDU2 also bind to the β-domain of pVHL and several naturally occurring mutations in the β-d[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
USP20 binds VHL and USP33. 2 / 2
| 2

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

sparser
"Based on their amino acid sequence homology and functional interaction with pVHL, VDU1 and VDU2 define a subfamily of ubiquitin specific processing proteases."
USP20 binds USP33 and AR. 1 / 1
| 1

sparser
"Because carvedilol enhanced β 2 AR ubiquitination and degradation independently of the known β 2 AR E3 ligase Nedd4, we reasoned that carvedilol might diminish β 2 AR deubiquitination by reducing the association of the β 2 AR with USP20 and/or USP33."
| 1

sparser
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
USP20 binds USP33 and ARs. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP20 affects HIF1A
3 1 | 11 3
USP20 binds HIF1A.
3 | 3 3
3 | 3 3

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."

sparser
"In contrast to VDU2 which directly interacts with HIF-1α, USP28 forms a ternary complex with HIF-1α via its association with HIF-1α bound Fbw7 (Flügel et al., xref )."

sparser
"According to those findings, USP20 binds to and stabilizes HIF-1α."

reach
"Molecular and cellular experiments showed that VDU2 interacted with HIF-1alpha in order to induce the expression of HIF-1 downstream genes, such as VEGF, through the deubiquitination and stabilization of HIF-1alpha."

No evidence text available

sparser
"However, by using in vitro pull down assays with GST-HIF-1α (amino acid 530–826) and co-immunoprecipitation experiments in COS-7 cells it was shown that only VDU2 but not VDU1 could interact with HIF-1α (Li et al., xref )."

No evidence text available

No evidence text available

reach
"The interaction between HIF-1alpha and VDU2 was shown using an immunoprecipitation assay."
USP20 deubiquitinates HIF1A.
1 | 4
USP20 deubiquitinates HIF1A. 5 / 5
1 | 4

"USP20 stabilizes HIF-1α by abolishing von Hippel-Lindau protein (pVHL)-E3 ligase complex-mediated HIF-1α degradation."

reach
"VDU2 can specifically deubiquitinate and stabilize HIF-1alpha and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF)."

reach
"In addition, VDU2, but not VDU1, is able to interact with and deubiquitinate HIF1alpha, and rescue it from proteasome mediated degradation."

reach
"In addition, it was shown that VDU2 but not VDU1 can deubiquitylate HIF-1alpha and increase it 's half-life."

reach
"One of them, USP-20, deubiquitinates HIF-1alpha and therefore tends to stabilize it [46] by preventing its proteasomal degradation."
USP20 activates HIF1A.
| 2
USP20 activates HIF1A. 2 / 4
| 2

reach
"Up-regulation of Usp20, a deubiquitinase that acts on Hif1a and other proteins 24, should reduce degradation of Hif1a."

reach
"Therefore, targeting USP20 can induce degradation of HIF-1alpha and be a potential drug target for human cancer."
USP20 increases the amount of HIF1A.
| 2
Modified USP20 increases the amount of HIF1A. 1 / 1
| 1

reach
"Thus, overexpression of VDU2 increases expression of HIF-1alpha targeted genes, such as VEGF."
USP20 increases the amount of HIF1A. 1 / 1
| 1

reach
"The previous study has shown that Usp20 increases the expression of HIF1A XREF_BIBR, XREF_BIBR, which is reported as a metabolic switch for an early stage of iPSC 20."
CLSPN affects USP20
7 | 5 5
CLSPN binds USP20.
7 | 4 5
7 | 4 4

reach
"To determine whether the interaction between USP20 and Claspin is direct, we generated and purified recombinant USP20 and Claspin."

reach
"However, depletion of HERC2 did not affect the binding between USP20 and Claspin."

No evidence text available

sparser
"We confirmed the USP20-Claspin interaction by Co-IP."

No evidence text available

No evidence text available

reach
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex and both USP20 and CLASPIN were in the HERC2 immunocomplex, suggesting that HERC2, USP20 and CLASPIN form a complex."

No evidence text available

reach
"As shown in Figure XREF_FIG, GST-USP20 was able to interact with His Claspin under cell-free conditions, suggesting a direct interaction between USP20 and Claspin."

sparser
"To determine whether the interaction between USP20 and Claspin is direct, we generated and purified recombinant USP20 and Claspin."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
CLSPN activates USP20.
| 1
CLSPN activates USP20. 1 / 1
| 1

reach
"Nevertheless, in USP20 knockdown cells, reconstitution of Claspin was able to rescue phospho-CHK1 levels and significantly suppress tumorigenesis, even if Rad17 levels was decreased, suggesting that Claspin is an important target of USP20 in mediating checkpoint activation and tumor suppression."
USP20 affects VHL
4 | 8 3
USP20 binds VHL.
4 | 7 3
4 | 7 1

No evidence text available

reach
"This mutation did not affect the interaction between hVDU2 and pVHL as predicted.VDU1 and VDU2 exhibit a very high homology at their N-terminal and C-terminal region as shown in Fig. 1, and both prote[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."

reach
"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The interaction between hVDU2 and pVHL was also tested by in vivo co-immunoprecipitation."

reach
"PVHL was precipitated by an anti-GFP antibody due to the interaction between hVDU2 and pVHL."

No evidence text available

reach
"Our data have demonstrated that several naturally occurring mutations in the pVHL beta-domain disrupt the interaction between hVDU2 and pVHL, which underscores the functional relevance of this interac[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 binds VHL and USP33. 2 / 2
| 2

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

sparser
"Based on their amino acid sequence homology and functional interaction with pVHL, VDU1 and VDU2 define a subfamily of ubiquitin specific processing proteases."
USP20 deubiquitinates VHL.
| 1
USP20 deubiquitinates ubiquitinated VHL. 1 / 1
| 1

reach
"Can VDU1 or VDU2 deubiquitinate the ubiquitinated pVHL to salvage it from degradation on certain conditions?"
USP20 affects ULK1
1 | 7 6
USP20 binds ULK1.
| 5 6
| 5 6

sparser
"USP20 can bind, de-ubiquitinate and stabilize ULK1 at a basal level and plays an important role in autophagy initiation."

reach
"USP20 interacts with and deubiquitinates ULK1, thus protecting it from degradation."

reach
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [XREF_BIBR]."

sparser
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [64]."

reach
"Under prolonged starvation, the binding of USP20 to ULK1 is diminished, leading to autophagy inhibition [43]."

sparser
"Under prolonged starvation, the binding of USP20 to ULK1 is diminished, leading to autophagy inhibition [ xref ]."

reach
"At basal state, USP20 binds to and stabilizes ULK1 by removing the ubiquitin moiety, thereby interfering with the lysosomal degradation of ULK1."

sparser
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [ xref ]."

sparser
"During prolonged starvation, NEDD4L catalyzes the K27 and K29 ubiquitination on ULK1 [ xref ], whereas the interaction between USP20 and ULK1 is attenuated [ xref ]."

reach
"During prolonged starvation, NEDD4L catalyzes the K27 and K29 ubiquitination on ULK1 [58], whereas the interaction between USP20 and ULK1 is attenuated [43]."
USP20 deubiquitinates ULK1.
1 | 1
USP20 deubiquitinates ULK1. 2 / 2
1 | 1

reach
"USP20 interacts with and deubiquitinates ULK1, thus protecting it from degradation."

"The deubiquitination of ULK1 by USP20 allows for its stabilization and promotion of autophagy induction."
USP20 ubiquitinates ULK1.
| 1
USP20 leads to the ubiquitination of ULK1. 1 / 1
| 1

reach
"The degradable ULK1 ubiquitination is reversed by USP20."
USP20 affects TRAF6
4 1 | 9
USP20 deubiquitinates TRAF6.
1 | 8
USP20 deubiquitinates TRAF6. 9 / 9
1 | 8

reach
"For example, Junichiro et al. demonstrated that USP21 constitutively deubiquitinates RIP1 in vitro and in vivo [40]; and a previous study has reported that USP20 deubiquitinates TRAF6 and Tax in vivo [40]."

reach
"For example, USP11 negatively regulates TNFα-induced NF-κB activation associated with IκBα and attenuates IκBα degradation [34]; USP20 deubiquitinates TRAF6 and suppresses interleukin 1β (IL-1β)- and Tax-induced NF-κB activation [40]; Katrin et al. showed that USP15 regulates IκBα/NF-κB by deubiquitinylation IκBα[44]; and USP31 inhibits TNFα, CD40, TRAF2, TRAF6 and IKKβ-mediated NF-κB activation [45]."

reach
"For example, Junichiro et al. demonstrated that USP21 constitutively deubiquitinates RIP1 in vitro and in vivo [XREF_BIBR]; and a previous study has reported that USP20 deubiquitinates TRAF6 and Tax in vivo [XREF_BIBR]."

reach
"We report that two ubiquitin specific peptidases, USP20 and USP33, deubiquitinate TRAF6 and suppress IL-1beta-induced NF-kappaB activation."
| PMC

reach
"Here, we report that ubiquitin specific peptidase USP20 deubiquitinates TRAF6 and Tax and suppresses interleukin 1beta (IL-1beta) - and Tax induced NF-kappaB activation."

reach
"54 Ubiquitinated beta-arrestin2 promotes NF-kappaB signaling in SMCs, whereas deubiquitinated beta-arrestin2 attenuates NF-kappaB signaling by scaffolding the deubiquitinase USP20, that in turn deubiquitinates TRAF6, which blocks the NF-kappaB signaling cascade."

reach
"TRAF6 activity can be impeded by deubiquitinating enzymes like ubiquitin specific protease 20 (USP20), which can reverse TRAF6 autoubiquitination, and by association with the multifunctional adaptor protein beta-arrestin2."

"With purified proteins and in intact cells, our protein interaction studies showed that TRAF6/USP20 association and subsequent USP20-mediated TRAF6 deubiquitination were β-arrestin2-dependent."

reach
"For example, USP11 negatively regulates TNFalpha induced NF-kappaB activation associated with IkappaBalpha and attenuates IkappaBalpha degradation [XREF_BIBR]; USP20 deubiquitinates TRAF6 and suppresses interleukin 1beta (IL-1beta) - and Tax induced NF-kappaB activation [XREF_BIBR]; Katrin et al. showed that USP15 regulates IkappaBalpha and NF-kappaB by deubiquitinylation IkappaBalpha [XREF_BIBR]; and USP31 inhibits TNFalpha, CD40, TRAF2, TRAF6 and IKKbeta mediated NF-kappaB activation [XREF_BIBR]."
USP20 binds TRAF6.
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP20 activates TRAF6.
| 1
USP20 activates TRAF6. 1 / 1
| 1

reach
"For example, USP17 and USP20 were found to target RIG-I and TRAF6 respectively, thereby functioning as novel regulators of antiviral innate immune responses [XREF_BIBR, XREF_BIBR]."
USP20 affects SQSTM1
| 1 12 1
| 1 12

sparser
"This is the first report that reveals the molecular mechanism of the USP20-p62 axis regarding NF-κB-mediated cell survival."

sparser
"Immunoprecipitation assays supported the endogenous interactions between USP20 and p62 upon TNFα treatment in HeLa cells ( xref B)."

sparser
"These findings strongly suggest that the USP20-p62 axis plays an essential role in NF-κB-mediated cell survival induced by the TNFα-atypical PKCζ signaling pathway."

sparser
"Our finding that USP20 stabilizes the p62 protein prompted us to examine whether USP20 directly binds to p62."

sparser
"USP20 specifically binds to p62 and acts as a positive regulator for NF-κB activation by TNFα through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival."

sparser
"Based on this speculation, our current experiments provide evidence that the p62-USP20 axis is required for PKCζ-mediated NF-κB activation upon TNFα treatment."

sparser
"This time point coincided with the timing of endogenous p62 interaction with USP20 ( xref B)."

sparser
"Co-immunoprecipitation assays revealed the direct interaction of USP20 with p62 when plasmids encoding HA-USP20 and Flag-p62 were ectopically expressed in HeLa cells ( xref A)."

sparser
"Based on this finding, we next examined the endogenous interaction of USP20 with p62 upon TNFα treatment."

sparser
"Herein, we find that USP20 binds to p62 at 5 min upon TNFα treatment and thus stabilizes it through removing the lysine 48 (K48)-linked polyubiquitin chains."
SQSTM1 binds USP20. 1 / 1
| 1

isi
"USP20 specifically binds to p62 and acts as a positive regulator for NF-kappaB activation by TNFalpha through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival."
USP20 affects STING1
2 1 | 1 5 4
USP20 binds STING1.
2 | 4
| 4

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."
2 |

No evidence text available

No evidence text available
USP20 inhibits STING1.
| 1 3
USP20 inhibits STING1. 4 / 4
| 1 3

eidos
"On the other hand , OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation , thus maintain the stability of STING and promote activation of STING pathway ( 87 ) ( 88 ) ( 89 ) ."

reach
"On the other hand, OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation, thus maintain the stability of STING and promote activation of STING pathway (87) (88) (89) ."

reach
"On the other hand, OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation, thus maintain the stability of STING and promote activation of STING pathway (87–89)."

reach
"USP18 deficiency or knockdown of USP20 resulted in enhanced K48 linked ubiquitination and accelerated degradation of STING, and impaired activation of IRF3 and NF-kappaB as well as induction of downstream genes after infection with DNA virus HSV-1 or transfection of various DNA ligands."
USP20 activates STING1.
| 2
USP20 activates STING1. 2 / 2
| 2

reach
"On the other hand, OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation, thus maintain the stability of STING and promote activation of STING pathway (87–89)."

reach
"On the other hand, OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation, thus maintain the stability of STING and promote activation of STING pathway (87) (88) (89) ."
USP20 deubiquitinates STING1.
1 |
USP20 deubiquitinates STING1. 1 / 1
1 |

"USP18 recruits USP20 to promote innate antiviral response through deubiquitinating STING/MITA"
USP20 affects USP18
| 10
| 6

sparser
"Immunoprecipitation revealed that STING, USP18 and USP20 are arranged as USP20-USP18-STING, but both USP20 and USP18 were associated with the N-terminus of STING [ xref ]."

sparser
"USP18-USP20 mediated the accumulation of STING that regulates oHSV-1 T1012G viral lytic replication in SCC9 cells."

sparser
"In 2016, Zhang et al. [61] reported that USP18 interacts with the deubiquitinase USP20 and recruits it to stimulator of interferon genes (STING)."

sparser
"Immunoprecipitation revealed that STING, USP18 and USP20 are arranged as USP20-USP18-STING, but both USP20 and USP18 were associated with the N-terminus of STING [41]."

sparser
"All the above results indicate that USP18-USP20 mediated the accumulation of STING function with limited oHSV-1 T1012G virus yields in SCC9 cells."

sparser
"In 2016, Zhang et al. [ xref ] reported that USP18 interacts with the deubiquitinase USP20 and recruits it to stimulator of interferon genes (STING)."
| 4

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."
USP20 affects CHEK1
| 8
USP20 activates CHEK1.
| 4
USP20 activates CHEK1. 4 / 8
| 4

reach
"USP20 promotes CHK1 activation in response to replication stress or UV radiation."

reach
"Taken together, these results indicate that USP20 promotes CHK1 activation in response to HU induced replication stress or UV irradiation."

reach
"To test if USP20 mediated stability of CLASPIN promotes CHK1 activation upon replication stress, we expressed wild-type FLAG-CLASPIN in USP20 depleted cells."

reach
"Upon replication stress, ATR mediated phosphorylation of USP20 promotes dissociation of HERC2 from USP20, stabilizes USP20 and its association with CLASPIN, thus increasing CALSPIN stability and ensuring CHK1 activation."
USP20 inhibits CHEK1.
| 2
USP20 inhibits CHEK1. 1 / 1
| 1

reach
"In a candidate screen of ubiquitin specific processing proteases (USPs) for modulating CHK1 activation, we revealed that inhibition of USP20 expression in 293T cells delayed HU induced CHK1 activation, whereas expression of siRNA resistant form of FLAG-USP20res in the endogenous USP20 depleted cells restored HU induced CHK1 activation kinetics."
USP20-C154S inhibits CHEK1. 1 / 1
| 1

reach
"Concomitantly, expression of the catalytically inactive mutant FLAG USP20 (C154S) attenuated HU induced CHK1 activation."
USP20 dephosphorylates CHEK1.
| 2
USP20 leads to the dephosphorylation of CHEK1. 2 / 2
| 2

reach
"As shown in Figure XREF_FIG, depletion of USP20 in cells dramatically reduced Chk1 phosphorylation following DNA damage."

reach
"Furthermore, depletion of USP20 can not further reduce Chk1 phosphorylation in Claspin knocking-down cells."
USP18 affects USP20
| 10
| 6

sparser
"Immunoprecipitation revealed that STING, USP18 and USP20 are arranged as USP20-USP18-STING, but both USP20 and USP18 were associated with the N-terminus of STING [ xref ]."

sparser
"USP18-USP20 mediated the accumulation of STING that regulates oHSV-1 T1012G viral lytic replication in SCC9 cells."

sparser
"In 2016, Zhang et al. [61] reported that USP18 interacts with the deubiquitinase USP20 and recruits it to stimulator of interferon genes (STING)."

sparser
"Immunoprecipitation revealed that STING, USP18 and USP20 are arranged as USP20-USP18-STING, but both USP20 and USP18 were associated with the N-terminus of STING [41]."

sparser
"All the above results indicate that USP18-USP20 mediated the accumulation of STING function with limited oHSV-1 T1012G virus yields in SCC9 cells."

sparser
"In 2016, Zhang et al. [ xref ] reported that USP18 interacts with the deubiquitinase USP20 and recruits it to stimulator of interferon genes (STING)."
| 4

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."
ULK1 affects USP20
| 5 6
| 5 6

sparser
"USP20 can bind, de-ubiquitinate and stabilize ULK1 at a basal level and plays an important role in autophagy initiation."

reach
"USP20 interacts with and deubiquitinates ULK1, thus protecting it from degradation."

reach
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [XREF_BIBR]."

sparser
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [64]."

reach
"Under prolonged starvation, the binding of USP20 to ULK1 is diminished, leading to autophagy inhibition [43]."

sparser
"Under prolonged starvation, the binding of USP20 to ULK1 is diminished, leading to autophagy inhibition [ xref ]."

reach
"At basal state, USP20 binds to and stabilizes ULK1 by removing the ubiquitin moiety, thereby interfering with the lysosomal degradation of ULK1."

sparser
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [ xref ]."

sparser
"During prolonged starvation, NEDD4L catalyzes the K27 and K29 ubiquitination on ULK1 [ xref ], whereas the interaction between USP20 and ULK1 is attenuated [ xref ]."

reach
"During prolonged starvation, NEDD4L catalyzes the K27 and K29 ubiquitination on ULK1 [58], whereas the interaction between USP20 and ULK1 is attenuated [43]."
GroL2 affects USP20
| 3 7
| 3 3

reach
"HSP56 interacts with both VDU1 and VDU2."

sparser
"We previously identified pVHL-interacting deubiquitinating enzyme 1 (VDU1) as a protein interacting with hSP56 xref , thus extended this finding to examine whether hSP56 interacts also with VDU2, a closely related isoform of VDU1."

reach
"We previously identified pVHL interacting deubiquitinating enzyme 1 (VDU1) as a protein interacting with hSP56 XREF_BIBR, thus extended this finding to examine whether hSP56 interacts also with VDU2, a closely related isoform of VDU1."

sparser
"Interestingly, we observed a stronger interaction between VDU2 and hSP56 than between VDU1 and hSP56 ( xref B)."

sparser
"These results suggest that the interaction of hSP56 with VDU2 is relevant."

reach
"Interestingly, we observed a stronger interaction between VDU2 and hSP56 than between VDU1 and hSP56 (XREF_FIG B)."
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."
USP20 affects NFkappaB
| 1 6 1 1
USP20 inhibits NFkappaB.
| 1 4 1
| 1 4 1

reach
"Silencing USP20 in SMCs with siRNA (small interfering RNA) augmented NFkappaB activation by = ~ 50% in response to either TNF or IL-1beta (interleukin-1beta)."

reach
"USP18 deficiency or knockdown of USP20 resulted in enhanced K48 linked ubiquitination and accelerated degradation of STING, and impaired activation of IRF3 and NF-kappaB as well as induction of downstream genes after infection with DNA virus HSV-1 or transfection of various DNA ligands."

reach
"XREF_BIBR USP20 inhibits NF-kappaB activation via Toll like receptor (TLR) 4, XREF_BIBR IL-1R, and the TNF XREF_BIBR inflammatory pathway in VSMCs."

reach
"XREF_BIBR This study showed that USP18 or USP20 deficiency significantly inhibited HSV-I or cytosolic DNA activation of both NF-kappaB and IRF3, and type-I IFN and proinflammatory cytokine expression."

sparser
"USPs are the most abundant DUBs, with approximately 60 proteases in humans. xref USP20 inhibits NF-κB activation via Toll-like receptor (TLR)4, xref IL-1R, and the TNF xref inflammatory pathway in VSMCs."

eidos
"Ubiquitin-specific peptidase 20 ( USP20 ) reduces NFkappaB activation triggered by pro-inflammatory stimuli , and USP20 activity attenuates atherosclerosis in mice ."
USP20 activates NFkappaB.
| 2
| 2

reach
"USP20 is downregulated in HTLV-1 infected cell lines, presumably to promote high levels of NF-kappaB activation, although the exact mechanism is unclear [XREF_BIBR]."

reach
"Coimmunoprecipitation experiments revealed that USP20 associates with several components of the TNFR1 (TNF receptor-1) signaling pathway, including RIPK1 (receptor interacting protein kinase 1), a critical checkpoint in TNF induced NFkappaB activation and inflammation."
USP20 binds NFkappaB.
| 1
NFkappaB binds USP20. 1 / 1
| 1

isi
"USP20 specifically binds to p62 and acts as a positive regulator for NF-kappaB activation by TNFalpha through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival."
PKA affects USP20
| 4 5 1
PKA phosphorylates USP20.
| 4 4 1
PKA phosphorylates USP20. 9 / 9
| 4 4 1

reach
"PKA dependent phosphorylation of the DUB USP20 was recently shown to perturb post-endocytic trafficking of the beta-andregenic receptor under cellular stress conditions."

reach
"Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of beta2 adrenergic receptors to autophagosomes during physiological stress."

rlimsp
"Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during physiological stress."

sparser
"Most recently, it was described that upon physiological stress, USP20 is phosphorylated by protein kinase A and this process regulates trafficking of β 2 AR to autophagosomes [ xref ]."

sparser
"Phosphorylation of USP20 by protein kinase A has been reported to regulate β2 adrenergic receptors intracellular trafficking ( xref )."

sparser
"PKA-dependent phosphorylation of the DUB USP20 was recently shown to perturb post-endocytic trafficking of the β-andregenic receptor under cellular stress conditions."

reach
"Phosphorylation of USP20 by protein kinase A has been reported to regulate β2 adrenergic receptors intracellular trafficking (Yu et al., 2019)."

reach
"Most recently, it was described that upon physiological stress, USP20 is phosphorylated by protein kinase A and this process regulates trafficking of beta 2 AR to autophagosomes [XREF_BIBR]."

sparser
"This process requires the protein kinase A-dependent phosphorylation and deactivation of the deubiquitinating enzyme USP20, allowing for the ubiquitination of β2AR to persist and act as a sorting signal to autophagosomes [ xref ]."
PKA inhibits USP20.
| 1
PKA inhibits USP20. 1 / 1
| 1

sparser
"Concomitant inhibition of the deubiquitinase USP20 (Ubiquitin-specific-processing protease 20) by PKA favors ubiquitylation and degradation of the receptor by NEDD4 ( xref ; xref )."
HIF1A affects USP20
3 | 3 3
3 | 3 3

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."

sparser
"In contrast to VDU2 which directly interacts with HIF-1α, USP28 forms a ternary complex with HIF-1α via its association with HIF-1α bound Fbw7 (Flügel et al., xref )."

sparser
"According to those findings, USP20 binds to and stabilizes HIF-1α."

reach
"Molecular and cellular experiments showed that VDU2 interacted with HIF-1alpha in order to induce the expression of HIF-1 downstream genes, such as VEGF, through the deubiquitination and stabilization of HIF-1alpha."

No evidence text available

sparser
"However, by using in vitro pull down assays with GST-HIF-1α (amino acid 530–826) and co-immunoprecipitation experiments in COS-7 cells it was shown that only VDU2 but not VDU1 could interact with HIF-1α (Li et al., xref )."

No evidence text available

No evidence text available

reach
"The interaction between HIF-1alpha and VDU2 was shown using an immunoprecipitation assay."

reach
"Silencing of USP20 increases proliferation in GC cells."

reach
"In HTLV-1-transformed cells, the transcription of USP20 is reduced compared with HTLV-1-negative T cells, and ectopic USP20 expression was found to inhibit the proliferation of an HTLV-1-transformed cell line, MT4."
| PMC

reach
"In line with the clinical outcomes, we found that the overexpression of USP20 could, on one hand, suppress cell proliferation and delay G1-S cell cycle transition, and on the other hand, enhance autophagy in GC cells."

reach
"Since overexpression of USP20 inhibits the proliferation of ATL cells, it raises the possibility that induction of USP20 could be used to inhibit HTLV induced oncogenesis."

reach
"Experimentally, small interfering RNA mediated knockdown of USP20 expression significantly promoted cell proliferation, accelerated G1-S phase transition and attenuated the autophagy activity."

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."

reach
"These finding revealed that USP20 could modulate GC cell proliferation."

reach
"Depletion of several DUBs caused either resistance or hypersensitivity to HU treatment; among them, USP20 knockdown showed most significant hypersensitivity to HU treatment, while knockdown of USP20 slightly decreased cell proliferation in untreated cells."
USP20 affects RAD17
4 1 | 2 1
USP20 binds RAD17.
4 | 1
4 | 1

No evidence text available

sparser
"We demonstrate that USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure."

No evidence text available

No evidence text available

No evidence text available
USP20 increases the amount of RAD17.
| 2
Modified USP20 increases the amount of RAD17. 1 / 1
| 1

reach
"It was also noted that inhibition of USP20 expression decreased RAD17 protein levels."
USP20 increases the amount of RAD17. 1 / 1
| 1

reach
"Consistent with this report, we found knockdown of USP20 in cells decreased Rad17 levels."
USP20 deubiquitinates RAD17.
1 |
USP20 deubiquitinates RAD17. 1 / 1
1 |

"Similar to Chk1 and Rad17, which enhance recombinational repair of collapsed replication forks, we demonstrate that USP20 depletion impairs DNA double strand break repair by homologous recombination."
USP20 affects CCP110
2 | 1 4
2 | 1 1

No evidence text available

sparser
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays ( xref ) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 ( xref )."

No evidence text available

reach
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
| 1

sparser
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
USP20 affects MAPK6
2 1 | 3
USP20 binds MAPK6.
2 | 1
2 | 1

reach
"USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."

No evidence text available

No evidence text available
USP20 deubiquitinates MAPK6.
1 | 1
USP20 deubiquitinates MAPK6. 2 / 2
1 | 1

"In this study, we carried out an unbiased functional loss-of-function screen of the human deubiquitinating enzyme (DUB) family and identified ubiquitin-specific protease 20 (USP20) as a novel ERK3 regulator.| USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."

reach
"USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."
USP20 decreases the amount of MAPK6.
| 1
USP20 decreases the amount of MAPK6. 1 / 1
| 1

reach
"The overexpression of USP20 results in the stabilization and accumulation of the ERK3 protein, whereas USP20 depletion reduces the levels of ERK3."
STING1 affects USP20
2 | 4
| 4

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."
2 |

No evidence text available

No evidence text available
RAD17 affects USP20
4 | 1 1
RAD17 binds USP20.
4 | 1
4 | 1

No evidence text available

sparser
"We demonstrate that USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure."

No evidence text available

No evidence text available

No evidence text available
RAD17 activates USP20.
| 1
RAD17 activates USP20. 1 / 1
| 1

reach
"Nevertheless, in USP20 knockdown cells, reconstitution of Claspin was able to rescue phospho-CHK1 levels and significantly suppress tumorigenesis, even if Rad17 levels was decreased, suggesting that Claspin is an important target of USP20 in mediating checkpoint activation and tumor suppression."
| 3

reach
"Our findings reveal novel mechanisms regulating IL-1β-induced pro-inflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation and neointimal hyperplasia."

reach
"Coimmunoprecipitation experiments revealed that USP20 associates with several components of the TNFR1 (TNF receptor-1) signaling pathway, including RIPK1 (receptor interacting protein kinase 1), a critical checkpoint in TNF induced NFkappaB activation and inflammation."

reach
"Phosphorylation of USP20 on Ser334 by IRAK-1 Promotes IL-1β-evoked Signaling in Vascular Smooth Muscle Cells and Vascular Inflammation."
| 1 1

reach
"USP20 (Ubiquitin Specific Protease 20) Inhibits TNF (Tumor Necrosis Factor)-Triggered Smooth Muscle Cell Inflammation and Attenuates Atherosclerosis."

sparser
"Thus, USP20 inhibits SMC inflammation in response to the atherogenic cytokine IL-1β, as it does to the atherogenic activation of TLR4. xref "
USP20 affects autophagy
| 5
| 4

reach
"Experimentally, small interfering RNA mediated knockdown of USP20 expression significantly promoted cell proliferation, accelerated G1-S phase transition and attenuated the autophagy activity."

reach
"Our result found that silencing of USP20 could inhibit autophagy, not singly but in pairs, the overexpression of USP33, which is the homology of USP20, could deubiquitinate RALB at Lys 47, and then regulate assembly of the RALB, EXO84, and beclin1 complex that initiated autophagy."

reach
"Thus, whether the autophagy caused by USP20 could contribute to the inhibition of tumor growth is worthy of investigation in a further study."

reach
"On the contrary, autophagy activity was enhanced by the USP20 overexpression (XREF_FIG)."
USP20 bound to ULK1 activates autophagy. 1 / 1
| 1

reach
"Under prolonged starvation, the binding of USP20 to ULK1 is diminished, leading to autophagy inhibition [43]."
USP20 affects USP20
| 1 4
USP20 inhibits USP20.
| 2
USP20 inhibits USP20. 2 / 2
| 2

reach
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

reach
"Our findings reveal novel mechanisms regulating IL-1β-induced pro-inflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation and neointimal hyperplasia."
USP20 activates USP20.
| 1 1
USP20 activates USP20. 2 / 2
| 1 1

eidos
"] Although deubiquitinating STING alone , USP20 can be recruited by USP18 , thus causing USP20 to remove K48-linked polyubiquitin chains more efficiently , even at a low dose ."

reach
"These suggest that ATR mediated phosphorylation of USP20 promotes dissociation of USP20 from HERC2, and this dissociation would stabilize CLASPIN and promote CHK1 activation."
USP20 phosphorylates USP20.
| 1
USP20 phosphorylates USP20 on S132. 1 / 1
| 1

reach
"Lu et al. demonstrated the post-prandial increase in insulin and the glucose concentration stimulates mTORC1 to phosphorylate the deubiquitylase ubiquitin-specific peptidase 20 (USP20) at S132 and S134, which is recruited to the HMGCR complex and antagonizes its degradation (Lu et al., 2020)."
USP20 affects Tax
| 5
USP20 deubiquitinates Tax. 5 / 5
| 5

reach
"Here, we report that ubiquitin specific peptidase USP20 deubiquitinates TRAF6 and Tax and suppresses interleukin 1beta (IL-1beta) - and Tax induced NF-kappaB activation."

reach
"We find that USP20 deubiquitinates Tax and inhibits Tax induced NF-kappaB activation, consistent with Tax being a substrate of USP20."
| PMC

reach
"For example, Junichiro et al. demonstrated that USP21 constitutively deubiquitinates RIP1 in vitro and in vivo [XREF_BIBR]; and a previous study has reported that USP20 deubiquitinates TRAF6 and Tax in vivo [XREF_BIBR]."

reach
"In further support of this, the deubiquitinating enzyme (DUB) USP20 deubiquitinates Tax to negatively regulate activation of IKK [XREF_BIBR]."

reach
"For example, Junichiro et al. demonstrated that USP21 constitutively deubiquitinates RIP1 in vitro and in vivo [40]; and a previous study has reported that USP20 deubiquitinates TRAF6 and Tax in vivo [40]."
USP20 affects TNF
| 4 1
USP20 inhibits TNF.
| 4
USP20 inhibits TNF. 4 / 4
| 4

reach
"USP20 (Ubiquitin Specific Protease 20) Inhibits TNF (Tumor Necrosis Factor)-Triggered Smooth Muscle Cell Inflammation and Attenuates Atherosclerosis."

reach
"XREF_BIBR USP20 inhibits NF-kappaB activation via Toll like receptor (TLR) 4, XREF_BIBR IL-1R, and the TNF XREF_BIBR inflammatory pathway in VSMCs."

reach
"Silencing USP20 in SMCs with siRNA (small interfering RNA) augmented NFkappaB activation by = ~ 50% in response to either TNF or IL-1beta (interleukin-1beta)."

reach
"Conclusions- USP20 attenuates TNF- and IL-1beta-evoked atherogenic signaling in SMCs, by deubiquitinating RIPK1, among other signaling intermediates."
USP20 binds TNF.
| 1
| 1

sparser
"Co-immunoprecipitation experiments revealed that USP20 associates with several components of the TNF receptor-1 signaling pathway including receptor-interacting protein kinase 1 (RIPK1), a critical checkpoint in TNF-induced NFκB activation and inflammation."
USP20 affects RIPK1
2 | 1 2
USP20 deubiquitinates RIPK1.
2 | 1
USP20 deubiquitinates RIPK1. 3 / 3
2 | 1

"RIPK1 was deubiquitinated by purified USP20 in vitro."

"Conclusions- USP20 attenuates TNF- and IL-1β-evoked atherogenic signaling in SMCs, by deubiquitinating RIPK1, among other signaling intermediates."

reach
"TNF evoked = ~ 2-fold more RIPK1 ubiquitination in SMC-DN-USP20-transgenic than in control SMCs, and RIPK1 was deubiquitinated by purified USP20 in vitro."
USP20 ubiquitinates RIPK1.
| 1
USP20 ubiquitinates RIPK1. 1 / 1
| 1

sparser
"Antagonizing USP20 activity in SMCs by low-level overexpression of DN-USP20 augmented TNF-induced RIPK1 ubiquitination ( xref )."
USP20 binds RIPK1.
| 1
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
USP20 affects NEURL4
3 | 2
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
USP20 affects ARRB2
| 3 2
USP20 deubiquitinates ARRB2.
| 2
USP20 deubiquitinates ARRB2. 2 / 2
| 2

reach
"Importantly, a double knockdown of USP20 and USP33 enhances the extent of beta-arrestin-2 ubiquitination and increases beta 2 AR degradation."

reach
"54 Ubiquitinated beta-arrestin2 promotes NF-kappaB signaling in SMCs, whereas deubiquitinated beta-arrestin2 attenuates NF-kappaB signaling by scaffolding the deubiquitinase USP20, that in turn deubiquitinates TRAF6, which blocks the NF-kappaB signaling cascade."
USP20 binds ARRB2.
| 2
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
| 1

sparser
"The association of USP20 with β-arrestin2 was augmented when β-arrestin2 ubiquitination was prevented and reduced when β-arrestin2 ubiquitination was rendered constitutive."
USP20 ubiquitinates ARRB2.
| 1
USP20 leads to the ubiquitination of ARRB2. 1 / 1
| 1

reach
"Importantly, a knockdown of USP20 and USP33 enhances beta-arrestin2 ubiquitination and increases beta 2 AR degradation [XREF_BIBR, XREF_BIBR]."
NEURL4 affects USP20
3 | 2
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
GroL2 affects USP33
| 4
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."
USP20 affects SOX4
| 1 2 1
USP20 binds SOX4.
| 1 1
| 1 1

sparser
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."

reach
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."
USP20 increases the amount of SOX4.
| 1
USP20 increases the amount of SOX4. 1 / 1
| 1

reach
"USP20 was found to modulate SOX4 protein expression."
USP20 activates SOX4.
| 1
USP20 activates SOX4. 1 / 1
| 1

eidos
"Finally , through rescue assays , we revealed that USP20 mediated SOX4 expression to accelerate CRC progression ."
USP20 affects PKM
1 | 1 2
USP20 binds PKM.
| 1 2
| 1 2

reach
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability."

sparser
"Apart from interacting with E3 ubiquitin ligases, PKM2 does also bind the deubiquitin enzyme USP20 (ubiquitin-specific protease, a deubiquitin enzyme)."

sparser
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability ( xref )."
USP20 deubiquitinates PKM.
1 |
USP20 deubiquitinates PKM. 1 / 1
1 |

"From this evidence, it is expected that USP20 would be associated with the metabolic pathway through the regulation of PKM2 ubiquitination."
USP18 affects STING1
| 4
| 4

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."
TRAF6 affects USP20
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
STING1 affects USP18, and USP20
| 4
| 4

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."
MAPK6 affects USP20
2 | 2
MAPK6 binds USP20.
2 | 1
2 | 1

reach
"USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."

No evidence text available

No evidence text available
MAPK6 activates USP20.
| 1
MAPK6 activates USP20. 1 / 1
| 1

reach
"Mechanistically, ERK3 expression level in cancer cells was shown to be upregulated by BRAF (through increasing ERK3 mRNA) 11, BMI1 (by suppressing let-7i which targets ERK3 mRNA) 12 and USP20 (by deubiquitinating and stabilizing ERK3 protein) 13."
3 |
Pirinixic acid increases the amount of USP20. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
VAPB affects USP20
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
VAPA affects USP20
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
USP33 affects CCP110
| 3
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
| 1

sparser
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
USP20 affects VAPB
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
USP20 affects VAPA
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
USP20 affects TNFRSF1A
| 3

sparser
"Thus, it appears that USP20 constitutively associates with components of the TNFR1 signaling complex to regulate NFκB signaling and inflammation."

sparser
"Coimmunoprecipitation experiments revealed that USP20 associates with several components of the TNFR1 (TNF receptor-1) signaling pathway, including RIPK1 (receptor-interacting protein kinase 1), a critical checkpoint in TNF-induced NFκB activation and inflammation."
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."

reach
"Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2."

reach
"USP20 enhances CRC cells metastasis and is associated with immune infiltration, immune checkpoints, and chemotherapy resistance."
USP20 affects NFKBIA
| 1 2
USP20 binds NFKBIA.
| 2
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
| 1

sparser
"For example, both β-arrestin2 xref and USP20 ( xref ) associate with IκBα; therefore, it is possible that a ternary complex of USP20, β-arrestin2 and IκBα facilitates deubiquitination of IκBα—a possibility made more plausible by the observation that β-arrestin2 and its homolog β-arrestin1 can inhibit IκBα degradation and NFκB signaling downstream of TNFR1. xref "
USP20 phosphorylates NFKBIA.
| 1
USP20 leads to the phosphorylation of NFKBIA. 1 / 1
| 1

reach
"Deletion of USP20 accelerates HSV-1-induced degradation of MITA and impairs phosphorylation of IRF3 and IkappaBalpha as well as subsequent induction of type I IFNs and proinflammatory cytokines after HSV-1 infection or cytoplasmic DNA challenge."
USP20 affects IRF3
| 3
USP20 inhibits IRF3.
| 2
USP20 inhibits IRF3. 2 / 2
| 2

reach
"XREF_BIBR This study showed that USP18 or USP20 deficiency significantly inhibited HSV-I or cytosolic DNA activation of both NF-kappaB and IRF3, and type-I IFN and proinflammatory cytokine expression."

reach
"USP18 deficiency or knockdown of USP20 resulted in enhanced K48 linked ubiquitination and accelerated degradation of STING, and impaired activation of IRF3 and NF-kappaB as well as induction of downstream genes after infection with DNA virus HSV-1 or transfection of various DNA ligands."
USP20 phosphorylates IRF3.
| 1
USP20 leads to the phosphorylation of IRF3. 1 / 1
| 1

reach
"Deletion of USP20 accelerates HSV-1-induced degradation of MITA and impairs phosphorylation of IRF3 and IkappaBalpha as well as subsequent induction of type I IFNs and proinflammatory cytokines after HSV-1 infection or cytoplasmic DNA challenge."
USP20 affects DIO2
1 1 1 |
USP20 binds DIO2.
1 1 |
1 1 |

No evidence text available

No evidence text available
USP20 deubiquitinates DIO2.
1 |
USP20 deubiquitinates DIO2. 1 / 1
1 |

"Ubiquitinated D2 can be either targeted to proteasomal degradation or reactivated by deubiquitination, a process that is mediated by the deubiquitinases USP20/33 and is important in adaptive thermogenesis."
| 2 1
| 2 1

eidos
"Ubiquitin-specific peptidase 20 ( USP20 ) reduces NFkappaB activation triggered by pro-inflammatory stimuli , and USP20 activity attenuates atherosclerosis in mice ."

reach
"Ubiquitin-specific peptidase 20 (USP20) reduces NFκB activation triggered by pro-inflammatory stimuli, and USP20 activity attenuates atherosclerosis in mice."

eidos
"147 In addition , USP20 attenuated atherosclerosis in an LDLr - / - mouse model by inhibiting NF-kappaB activation in vivo.148 Therefore , increased expression / activity of USP20 may offer protection against inflammatory pathways in patients with ISR ."
USP20 affects AR
| 2 1
USP20 ubiquitinates AR.
| 1
USP20 leads to the ubiquitination of AR. 1 / 1
| 1

reach
"Ubiquitination of the beta 2 AR is reversed by the deubiquitinases USP20 and USP33, which tonically associate with the beta 2 AR."
USP20 deubiquitinates AR.
| 1
USP20 leads to the deubiquitination of AR. 1 / 1
| 1

reach
"104 Two DUBs USP20 and USP33 have been shown to mediate deubiquitination of beta 2 AR."
USP20 binds AR.
| 1
USP20 binds USP33 and AR. 1 / 1
| 1

sparser
"Because carvedilol enhanced β 2 AR ubiquitination and degradation independently of the known β 2 AR E3 ligase Nedd4, we reasoned that carvedilol might diminish β 2 AR deubiquitination by reducing the association of the β 2 AR with USP20 and/or USP33."
USP20 affects ADRB2
2 1 |
USP20 binds ADRB2.
2 |
2 |

No evidence text available

No evidence text available
USP20 deubiquitinates ADRB2.
1 |
USP20 deubiquitinates ADRB2. 1 / 1
1 |
TNFRSF1A affects USP20
| 3

sparser
"Thus, it appears that USP20 constitutively associates with components of the TNFR1 signaling complex to regulate NFκB signaling and inflammation."

sparser
"Coimmunoprecipitation experiments revealed that USP20 associates with several components of the TNFR1 (TNF receptor-1) signaling pathway, including RIPK1 (receptor-interacting protein kinase 1), a critical checkpoint in TNF-induced NFκB activation and inflammation."
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."
PKM affects USP20
| 1 2
| 1 2

reach
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability."

sparser
"Apart from interacting with E3 ubiquitin ligases, PKM2 does also bind the deubiquitin enzyme USP20 (ubiquitin-specific protease, a deubiquitin enzyme)."

sparser
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability ( xref )."
CCP110 affects USP33
| 3
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
| 1

sparser
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
VSX2 affects USP20
2 |
2 |

No evidence text available

No evidence text available
VHL affects USP33
| 2
USP20 binds VHL and USP33. 2 / 2
| 2

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

sparser
"Based on their amino acid sequence homology and functional interaction with pVHL, VDU1 and VDU2 define a subfamily of ubiquitin specific processing proteases."
USP33 affects NEURL4
| 2
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
USP21 affects USP20
2 |
2 |

No evidence text available

No evidence text available

reach
"Genetic deletion or pharmacological inhibition of USP20 markedly decreases diet induced body weight gain, reduces lipid levels in the serum and liver, improves insulin sensitivity and increases energy expenditure."

reach
"USP20 links feeding induced cholesterol synthesis and energy expenditure."
USP20 affects cell cycle
| 2
| 2

reach
"As shown in XREF_FIG, the silencing of USP20 expression accelerated the G1-S cell cycle transition which is accompanied by decrease in the percentage of G1 cells (down to 54.05%) and increase in the percentage of cells in the S phase (up to 37.62%), and the tendency was also consistent with MKN45-siUSP20 cells and BGC-823-siUSP20 cells (P < 0.05; XREF_FIG and XREF_FIG)."

reach
"In line with the clinical outcomes, we found that the overexpression of USP20 could, on one hand, suppress cell proliferation and delay G1-S cell cycle transition, and on the other hand, enhance autophagy in GC cells."
USP20 affects VSX2
2 |
2 |

No evidence text available

No evidence text available
USP20 affects USP33, and VHL
| 2
USP20 binds VHL and USP33. 2 / 2
| 2

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."

sparser
"Based on their amino acid sequence homology and functional interaction with pVHL, VDU1 and VDU2 define a subfamily of ubiquitin specific processing proteases."
USP20 affects USP21
2 |
2 |

No evidence text available

No evidence text available
| 2
| 1

reach
"Silencing of USP20 increases proliferation in GC cells."
| 1

reach
"These finding revealed that USP20 could modulate GC cell proliferation."
USP20 affects MCL1
| 1 1
USP20 deubiquitinates MCL1.
| 1
USP20 leads to the deubiquitination of MCL1. 1 / 1
| 1

reach
"In addition, depletion of USP20 could increase the polyubiquitination of MCL1, thereby increasing the conversion rate of MCL1 and the sensitivity of cells to chemotherapy."
USP20 binds MCL1.
| 1
| 1

sparser
"Overall, our findings indicate that the USP20-MCL1 axis might play a key role in the apoptotic signaling pathway."
USP20 affects K33-
| 2
USP20 deubiquitinates K33- on K48. 2 / 2
| 2

reach
"USP20 deubiquitinated K33- or K48 linked ubiquitin of STING [XREF_BIBR]."

reach
"USP20 deubiquitinated K33- or K48-linked ubiquitin of STING [41]."
USP20 affects IL1B
| 1 1
USP20 inhibits IL1B. 2 / 2
| 1 1

eidos
"For example , USP11 negatively regulates TNFalpha-induced NF-kappaB activation associated with IkappaBalpha and attenuates IkappaBalpha degradation [ 34 ] ; USP20 deubiquitinates TRAF6 and suppresses interleukin 1beta ( IL-1beta ) - and Tax-induced NF-kappaB activation [ 40 ] ; Katrin et al. showed that USP15 regulates IkappaBalpha / NF-kappaB by deubiquitinylation IkappaBalpha [ 44 ] ; and USP31 inhibits TNFalpha , CD40 , TRAF2 , TRAF6 and IKKbeta-mediated NF-kappaB activation [ 45 ] ."

reach
"Silencing USP20 in SMCs with siRNA (small interfering RNA) augmented NFkappaB activation by = ~ 50% in response to either TNF or IL-1beta (interleukin-1beta)."
USP20 affects IKKalpha beta phosphorylation
| 2
USP20 activates IKKalpha beta phosphorylation. 2 / 2
| 2

eidos
"In addition , USP20 depletion significantly decreased IKKalpha / beta phosphorylation and delayed IkappaBalpha degradation , resulting in the reduction of NF-kappaB-mediated cell survival ."

eidos
"Furthermore , USP20 depletion significantly reduced IKKalpha / beta phosphorylation and delayed IkappaBalpha degradation in the presence of TNFalpha ( Figure 3E ) , which was similarly observed in p62-depleted HeLa cells ( Figure S1 ) ."
USP20 affects IFN-I
| 1 1
USP20 activates IFN-I. 2 / 2
| 1 1

eidos
"Among the DUBs that interact with STING , five members , namely CYLD , OTUD5 , USP18 ( also termed UBP43 ) , USP20 , and USP44 , have been demonstrated to promote IFN-I production and antiviral responses ."

reach
"Among the DUBs that interact with STING, five members, namely CYLD, OTUD5, USP18 (also termed UBP43), USP20, and USP44, have been demonstrated to promote IFN-I production and antiviral responses."
USP20 affects HSPA2
| 2
| 2

sparser
"Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice."

sparser
"The downregulation of the USP20-HSPA2 axis in diet-induced obese mice following SG improved lipid dysmetabolism, indicating that USP20-HSPA2 axis was a noninvasive therapeutic target to be investigated in the future."
USP20 affects DBNL
2 |
2 |

No evidence text available

No evidence text available

reach
"USP18 and USP20 restrict oHSV-1 replication in resistant human oral squamous carcinoma cell line SCC9 and affect the viability of SCC9 cells."

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."
USP20 affects CNTN2
| 2
USP20 deubiquitinates CNTN2.
| 1
USP20 deubiquitinates CNTN2. 1 / 1
| 1

reach
"The ubiquitin specific peptidase USP20 (ubiquitin specific peptidase) deubiquitinates Tax-1 and suppresses IL-1beta- and Tax-1-induced NF-kappaB activation XREF_BIBR."
USP20 binds CNTN2.
| 1
| 1

reach
"Tax-1 interacts with NRP and optineurin and TAX1BP1, and with the ubiquitin specific peptidase USP20."
USP20 affects CBX7
2 |
2 |

No evidence text available

No evidence text available
USP20 affects APBA2
2 |
2 |

No evidence text available

No evidence text available
SOX4 affects USP20
| 1 1
| 1 1

sparser
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."

reach
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."
PTEN affects USP20
| 1 1
PTEN binds USP20.
| 1
| 1

sparser
"Mechanistically, we found that USP20 directly interacted with PTEN."
PTEN activates USP20.
| 1
PTEN activates USP20. 1 / 1
| 1

reach
"Notably, suppressing PTEN with its specific inhibitor dramatically abolished the function of USP20 to ameliorate neuroinflammation and neuron death induced by OGD/R."
PRKACA affects USP20
1 1 |
PRKACA phosphorylates USP20.
1 |
PRKACA phosphorylates USP20 on S333. 1 / 1
1 |

No evidence text available
PRKACA binds USP20.
1 |
1 |

No evidence text available
NFKBIA affects USP20
| 2
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
| 1

sparser
"For example, both β-arrestin2 xref and USP20 ( xref ) associate with IκBα; therefore, it is possible that a ternary complex of USP20, β-arrestin2 and IκBα facilitates deubiquitination of IκBα—a possibility made more plausible by the observation that β-arrestin2 and its homolog β-arrestin1 can inhibit IκBα degradation and NFκB signaling downstream of TNFR1. xref "
NEURL4 affects USP33
| 2
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
IRAK1 affects USP20
| 2
IRAK1 phosphorylates USP20 on S333. 1 / 1
| 1

sparser
"Phosphorylation of USP20 on Ser334 by IRAK-1 Promotes IL-1β-evoked Signaling in Vascular Smooth Muscle Cells and Vascular Inflammation."
IRAK1 phosphorylates USP20 on S333. 1 / 1
| 1

sparser
"Phosphorylation of USP20 on Ser334 by IRAK-1 Promotes IL-1β-evoked Signaling in Vascular Smooth Muscle Cells and Vascular Inflammation."
HSPA2 affects USP20
| 2
| 2

sparser
"Sleeve gastrectomy improves lipid dysmetabolism by downregulating the USP20-HSPA2 axis in diet-induced obese mice."

sparser
"The downregulation of the USP20-HSPA2 axis in diet-induced obese mice following SG improved lipid dysmetabolism, indicating that USP20-HSPA2 axis was a noninvasive therapeutic target to be investigated in the future."
DIO2 affects USP20
1 1 |
1 1 |

No evidence text available

No evidence text available
DBNL affects USP20
2 |
2 |

No evidence text available

No evidence text available
CCP110 affects NEURL4, USP20, and USP33
| 2
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
CBX7 affects USP20
2 |
2 |

No evidence text available

No evidence text available
| 1

rlimsp
"Upon β2AR activation, a specific isoform of the second messenger cAMP-dependent protein kinase A (PKAα) rapidly phosphorylates USP20 on serine 333 located in its unique insertion domain. This phosphorylation of USP20 correlates with a characteristic SDS-PAGE mobility shift of the protein, blocks its deubiquitinase activity, promotes its dissociation from the activated β2AR complex, and facilitates trafficking of the ubiquitinated β2AR to autophagosomes, which fuse with lysosomes to form autolysosomes where receptors are degraded."
Ataxia Telangiectasia phosphorylates USP20 on S333. 1 / 1
| 1

rlimsp
"Upon β2AR activation, a specific isoform of the second messenger cAMP-dependent protein kinase A (PKAα) rapidly phosphorylates USP20 on serine 333 located in its unique insertion domain."
ARRB2 affects USP20
| 2
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
| 1

sparser
"The association of USP20 with β-arrestin2 was augmented when β-arrestin2 ubiquitination was prevented and reduced when β-arrestin2 ubiquitination was rendered constitutive."
APBA2 affects USP20
2 |
2 |

No evidence text available

No evidence text available
ADRB2 affects USP20
2 |
2 |

No evidence text available

No evidence text available
3,4-methylenedioxymethamphetamine increases the amount of USP20.
1 |
1 |

No evidence text available
3,4-methylenedioxymethamphetamine decreases the amount of USP20.
1 |
1 |

No evidence text available
Urethane affects USP20
1 |
Urethane increases the amount of USP20. 1 / 1
1 |

No evidence text available
Tetrachloromethane decreases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Sodium arsenite increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Silicon dioxide decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Protein kinase A-alpha affects USP20
| 1
Protein kinase A-alpha phosphorylates USP20 on S333. 1 / 1
| 1

reach
"beta1AR induced phosphorylation of USP20 Ser333 by protein kinase A-alpha (PKAalpha) was required for optimal USP20 mediated regulation of beta1AR lysosomal trafficking."
1 |
Progesterone increases the amount of USP20. 1 / 1
1 |

No evidence text available
Potassium chromate increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Phenobarbital increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
1 |

No evidence text available
1 |
Methamphetamine increases the amount of USP20. 1 / 1
1 |

No evidence text available
MTORC1 affects USP20
| 1
MTORC1 phosphorylates USP20. 1 / 1
| 1

sparser
"In contrast to ubiquitination, HMGCR is deubiquitinated by mTORC1-phosphorylated USP20, which preferentially hydrolyzes K48 and K63 linkages, and stabilized HMGCR increases cholesterol synthesis in the feeding state (Lu et al., 2020)."
Lipopolysaccharide increases the amount of USP20. 1 / 1
1 |

No evidence text available
Leflunomide affects USP20
1 |
Leflunomide decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Interaction affects USP20
| 1
Interaction activates USP20. 1 / 1
| 1

eidos
"As a consequence of this interaction , USP20 induces the expression of HIF-1alpha target genes , such as vascular endothelial growth factor ( VEGF ) ."
1 |
Hydroperoxide decreases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-26b-5p decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Genistein affects USP20
1 |
Genistein decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Flutamide affects USP20
1 |
Flutamide increases the amount of USP20. 1 / 1
1 |

No evidence text available
Ethoxyimino H-indeno b pyrazine-2 dicarbonitrile affects USP20
| 1
Ethoxyimino H-indeno b pyrazine-2 dicarbonitrile inhibits USP20. 1 / 1
| 1

eidos
"For example , 8-mercapto-N - ( ( tetrahydro-3-furanyl ) methyl ) -4 - quinoline carboxamide , LND-57444 , VLX1570 , ML323 , ( ADC-01 , ADC-03 , HBX41108 , HBX19818 , P5091 , P22077 ) , 9 - ( ethoxyimino ) -9 H-indeno ( 1,2 - b ) pyrazine-2 ,3 - dicarbonitrile , WP1130 , Mitoxantrone and GSK2643943A are able to inhibit PSMD14 , UCHL1 , UCHL5 and USP14 , USP1 , USP7 , USP8 , USP9X , USP11 and USP20 , respectively ."
1 |
Carbon nanotube decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Caffeine affects USP20
| 1
Caffeine leads to the dephosphorylation of USP20. 1 / 1
| 1

reach
"Using an antibody against consensus ATM and ATR phosphorylation sites (anti-phospho-SQ/TQ), we found that phosphorylation of USP20 at SQ/TQ motifs following HU or UV treatment and USP20 phosphorylation was blocked by caffeine."
Bisphenol A affects USP20
1 |
Bisphenol A decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Bis(2-ethylhexyl) phthalate increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
1 |

No evidence text available
1 |
Benzo[a]pyrene increases the amount of USP20. 1 / 1
1 |

No evidence text available
All-trans-retinoic acid increases the amount of USP20. 1 / 1
1 |

No evidence text available

sparser
"USP20 is also associated with β2 adrenergic receptor recycling."
ZNF770 affects USP20
1 |
1 |

No evidence text available
WP1130 affects USP20
| 1
WP1130 inhibits USP20. 1 / 1
| 1

eidos
"For example , 8-mercapto-N - ( ( tetrahydro-3-furanyl ) methyl ) -4 - quinoline carboxamide , LND-57444 , VLX1570 , ML323 , ( ADC-01 , ADC-03 , HBX41108 , HBX19818 , P5091 , P22077 ) , 9 - ( ethoxyimino ) -9 H-indeno ( 1,2 - b ) pyrazine-2 ,3 - dicarbonitrile , WP1130 , Mitoxantrone and GSK2643943A are able to inhibit PSMD14 , UCHL1 , UCHL5 and USP14 , USP1 , USP7 , USP8 , USP9X , USP11 and USP20 , respectively ."
USP33 affects ARs
| 1
USP20 binds USP33 and ARs. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP33 affects AR
| 1
USP20 binds USP33 and AR. 1 / 1
| 1

sparser
"Because carvedilol enhanced β 2 AR ubiquitination and degradation independently of the known β 2 AR E3 ligase Nedd4, we reasoned that carvedilol might diminish β 2 AR deubiquitination by reducing the association of the β 2 AR with USP20 and/or USP33."
USP28 affects USP20
1 |
1 |

No evidence text available
1 |
1 |

No evidence text available
USP20 affects ubiquitinated HIF-1alpha
| 1
USP20 activates ubiquitinated HIF-1alpha. 1 / 1
| 1

reach
"These findings suggest that ubiquitination of HIF-1alpha is a dynamic process and that ubiquitinated HIF-1alpha might be rescued from degradation by VDU2 through deubiquitination."
USP20 affects type I IFNs
| 1
USP20 inhibits type I IFNs. 1 / 1
| 1

reach
"Consistently, Usp20 -/- mice produce decreased type I IFNs and proinflammatory cytokines, exhibit increased susceptibility to lethal HSV-1 infection, and aggravated HSV-1 replication compared with Usp20 +/+ mice."
USP20 affects stability STING
| 1
USP20 activates stability STING. 1 / 1
| 1

eidos
"Mechanistically , USP20 is recruited by USP18 to deconjugate K48-linked ubiquitination chains from STING and thus promotes the stability of STING and the expression of type I IFNs and proinflammatory cytokines after DNA virus infection ( 91 ) ."
USP20 affects signaling
| 1
USP20 inhibits signaling. 1 / 1
| 1

sparser
"Thus, RIPK1 itself appears to be a substrate of USP20—and, by directly deubiquitinating RIPK1, USP20 can inhibit TNFR1-triggered NFκB signaling."
USP20 affects signaling complex composed p62 PKCzeta RIPK1
| 1
USP20 activates signaling complex composed p62 PKCzeta RIPK1. 1 / 1
| 1

eidos
"To validate this possibility , we examined whether USP20 depletion disrupts formation of the signaling complex composed of p62 , PKCzeta and RIPK1 upon TNFalpha treatment ."
USP20 affects severity cerebral ischemic stroke
| 1
USP20 inhibits severity cerebral ischemic stroke. 1 / 1
| 1

eidos
"Notably , suppressing PTEN with its specific inhibitor dramatically abolished the function of USP20 to ameliorate neuroinflammation and neuron death induced by OGD / R. Collectively , our results illustrated that USP20 could effectively mitigate the severity of cerebral ischemic stroke and improve behavior deficits in MCAO-operated mice , and identified the USP20 / PTEN axis as a promising therapeutic target for ischemic stroke treatment ."
USP20 affects sensitivity cells chemotherapy
| 1
USP20 inhibits sensitivity cells chemotherapy. 1 / 1
| 1

eidos
"In addition , depletion of USP20 could increase the polyubiquitination of MCL1 , thereby increasing the conversion rate of MCL1 and the sensitivity of cells to chemotherapy ."
USP20 affects post-endocytic trafficking autophagosomes
| 1
USP20 activates post-endocytic trafficking autophagosomes. 1 / 1
| 1

eidos
"However , solely USP20 was shown to promote the deubiquitination of beta2-ARs and their post-endocytic trafficking to autophagosomes ."
USP20 affects polyubiquitination MCL1
| 1
USP20 inhibits polyubiquitination MCL1. 1 / 1
| 1

eidos
"In addition , depletion of USP20 could increase the polyubiquitination of MCL1 , thereby increasing the conversion rate of MCL1 and the sensitivity of cells to chemotherapy ."
USP20 affects pVHL-E3 ligase
| 1
USP20 deubiquitinates pVHL-E3 ligase. 1 / 1
| 1

reach
"Can VDU1 and VDU2 deubiquitinate the ubiquitinated downstream targets of pVHL-E3 ligase, such as HIF-1alpha, therefore, counteract or modify the ubiquitinating function of VCB-E3 ligase?"
USP20 affects p62 protein
| 1
USP20 activates p62 protein. 1 / 1
| 1

eidos
"USP20 depletion did not affect the levels of p62 mRNA ( Figure 1A ) , but significantly decreased the expression of p62 protein ( Figure 1B ) ."
USP20 affects neointimal hyperplasia caused carotid endothelial denudation transgenic mice model
| 1
USP20 inhibits neointimal hyperplasia caused carotid endothelial denudation transgenic mice model. 1 / 1
| 1

eidos
"Intriguingly , USP20 impedes TLR4-induced NF-kappaB activation by deubiquitinating TNF receptor-associated factor 6,147 whereas it inhibits IL-1R and TNF-triggered NF-kappaB activation by deubiquitinating receptor-interacting protein kinase 1.148 Consistent with the results of the in vitro experiments , USP20 decreased neointimal hyperplasia caused by carotid endothelial denudation in a transgenic mice model ."
USP20 affects ischemic stroke mice
| 1
USP20 inhibits ischemic stroke mice. 1 / 1
| 1

eidos
"USP20 mitigates ischemic stroke in mice by suppressing neuroinflammation and neuron death via regulating PTEN signal ."

reach
"VDU1 and VDU2, a closely related isoform, have been shown to increase half-life of type 2 iodothyronine deiodinase by their deubiquitinating activity [23]."

reach
"USP20 also deubiquitinates hypoxia inducible factor-1 alpha (HIF-1alpha), promoting HIF-1alpha stability and consequently the expression of its target genes."
USP20 affects deubiquitination beta2-ARs
| 1
USP20 activates deubiquitination beta2-ARs. 1 / 1
| 1

eidos
"However , solely USP20 was shown to promote the deubiquitination of beta2-ARs and their post-endocytic trafficking to autophagosomes ."
USP20 affects conversion MCL1
| 1
USP20 inhibits conversion MCL1. 1 / 1
| 1

eidos
"In addition , depletion of USP20 could increase the polyubiquitination of MCL1 , thereby increasing the conversion rate of MCL1 and the sensitivity of cells to chemotherapy ."
USP20 affects cleaved caspase-8 caspase-3 PARP TNFalpha CHX compared control HeLa cells causing basal cleaved proteins
| 1
USP20 inhibits cleaved caspase-8 caspase-3 PARP TNFalpha CHX compared control HeLa cells causing basal cleaved proteins. 1 / 1
| 1

eidos
"In addition , immunoblot analysis showed that USP20 depletion increases the expression of cleaved caspase-8 , caspase-3 and PARP in the presence of TNFalpha / CHX , compared to control HeLa cells , as well as causing increased basal levels of these cleaved proteins without TNFalpha / CHX ( Figure 4D ) ."
USP20 affects cholesterol
| 1
| 1

reach
"USP20 links feeding induced cholesterol synthesis and energy expenditure."
USP20 affects cell migration invasion breast cancer
| 1
USP20 activates cell migration invasion breast cancer. 1 / 1
| 1

eidos
"USP4 and USP20 can promote the cell migration and invasion activities in breast cancer by inhibiting NFkappaB activation via deubiquitination of TRAF2 and TRAF6 [ 74 ] ."
USP20 affects cell growth
| 1
| 1

reach
"As shown in XREF_FIG, silencing of USP20 significantly promoted cell growth in monolayer culture, and further experimental results revealed that the proliferation ability of MGC-803-USP20 cells were lower than that of MGC-803-NC cells (XREF_FIG)."
USP20 affects cell death
| 1
| 1

eidos
"Because our results suggest that the USP20-p62 axis is responsible for PKCzeta-mediated NF-kappaB activation for cell survival , USP20 depletion may promote cell death ."
USP20 affects cell death pathways
| 1
USP20 inhibits cell death pathways. 1 / 1
| 1

eidos
"Based on our data that USP20 is responsible for NF-kappaB-mediated cell survival through stabilizing p62 , we also suggest that USP20 depletion causes the blockade of TNFalpha-induced NF-kappaB activation regarding PKCzeta and thus may accelerate cell death pathways ."
USP20 affects beta2AR
| 1
USP20 activates beta2AR. 1 / 1
| 1

reach
"Conversely, depletion of both USP33 and USP20 was shown to increase downregulation of the beta2AR, suggesting a specific role of these DUBs in regulating recycling of this GPCR, in a manner similar to that described for AMSH on the EGFR."
USP20 affects beta2-ARs
| 1
USP20 leads to the deubiquitination of beta2-ARs. 1 / 1
| 1

reach
"However, solely USP20 was shown to promote the deubiquitination of beta2-ARs and their post-endocytic trafficking to autophagosomes."
USP20 affects beta-arrestins
| 1
USP20 leads to the deubiquitination of beta-arrestins. 1 / 1
| 1

reach
"Deubiquitination of beta-arrestins is also regulated by agonist activation of the beta2AR and other GPCRs and is mediated by USP20 and USP33 and is important for beta, arrestin, and beta2AR complex disassembly, lysosomal sorting and signaling [XREF_BIBR, XREF_BIBR] (XREF_FIG)."
USP20 affects beta-arrestin
| 1
USP20 activates beta-arrestin. 1 / 1
| 1

reach
"The closely related DUBs, USP20 and USP33, deubiquitylate activated beta-adrenergic receptors as well as the adaptor and signalling protein beta-arrestin (in the case of USP33), to favour recycling from endosomes, despite largely being associated with the secretory pathway 75, 76, 77."
USP20 affects beta-andregenic receptor
| 1
USP20 inhibits beta-andregenic receptor. 1 / 1
| 1

reach
"PKA dependent phosphorylation of the DUB USP20 was recently shown to perturb post-endocytic trafficking of the beta-andregenic receptor under cellular stress conditions."
USP20 affects behavior
| 1
| 1

eidos
"Notably , suppressing PTEN with its specific inhibitor dramatically abolished the function of USP20 to ameliorate neuroinflammation and neuron death induced by OGD / R. Collectively , our results illustrated that USP20 could effectively mitigate the severity of cerebral ischemic stroke and improve behavior deficits in MCAO-operated mice , and identified the USP20 / PTEN axis as a promising therapeutic target for ischemic stroke treatment ."
USP20 affects atypical PKCzeta-RIPK1-p62 complex required NF-kappaB activation
| 1
USP20 activates atypical PKCzeta-RIPK1-p62 complex required NF-kappaB activation. 1 / 1
| 1

eidos
"Furthermore , depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor ."
USP20 affects apoptosis induced cycloheximide TNFalpha TAK1
| 1
USP20 activates apoptosis induced cycloheximide TNFalpha TAK1. 1 / 1
| 1

eidos
"Furthermore , depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor ."
USP20 affects antiviral responses
| 1
USP20 activates antiviral responses. 1 / 1
| 1

eidos
"Among the DUBs that interact with STING , five members , namely CYLD , OTUD5 , USP18 ( also termed UBP43 ) , USP20 , and USP44 , have been demonstrated to promote IFN-I production and antiviral responses ."

sparser
"USP20 is also associated with β2 adrenergic receptor recycling."
USP20 affects activity
| 1
USP20 inhibits activity. 1 / 1
| 1

sparser
"To identify potential substrates of USP20 and elucidate mechanisms by which USP20 inhibits TNF-evoked NFκB activity, we immunoprecipitated HA-tagged USP20 from HEK-293 cells and immunoblotted for endogenous proteins that constitute TNFR1-activated signaling intermediates."
USP20 affects activation
| 1
USP20 inhibits activation. 1 / 1
| 1

sparser
"Furthermore, host DUBs that interact with HTLV-1Tax oncoprotein were investigated, and consequently ubiquitin-specific peptidase USP20 was found to deubiquitinate Tax and inhibit Tax-induced NF-κB activation [ xref ]."
USP20 affects activation STING pathway
| 1
USP20 activates activation STING pathway. 1 / 1
| 1

eidos
"On the other hand , OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation , thus maintain the stability of STING and promote activation of STING pathway ( 87 ) ( 88 ) ( 89 ) ."
USP20 affects ZNF770
1 |
1 |

No evidence text available
USP20 affects USP28
1 |
1 |

No evidence text available
USP20 affects USP18-induced deubiquitination STING knockdown
| 1
USP20 activates USP18-induced deubiquitination STING knockdown. 1 / 1
| 1

eidos
"Subsequently , they searched for DUBs that interact with USP18 and found that knockdown of USP20 inhibited USP18-induced deubiquitination of STING and knockdown of USP18 inhibited USP20-induced deubiquitination of STING [ 41 ] ."
USP20 affects USP15
1 |
1 |

No evidence text available
USP20 affects UCHL3
1 |
1 |

No evidence text available
USP20 affects Tax-induced NF-kappaB activation
| 1
USP20 inhibits Tax-induced NF-kappaB activation. 1 / 1
| 1

eidos
"For example , USP11 negatively regulates TNFalpha-induced NF-kappaB activation associated with IkappaBalpha and attenuates IkappaBalpha degradation [ 34 ] ; USP20 deubiquitinates TRAF6 and suppresses interleukin 1beta ( IL-1beta ) - and Tax-induced NF-kappaB activation [ 40 ] ; Katrin et al. showed that USP15 regulates IkappaBalpha / NF-kappaB by deubiquitinylation IkappaBalpha [ 44 ] ; and USP31 inhibits TNFalpha , CD40 , TRAF2 , TRAF6 and IKKbeta-mediated NF-kappaB activation [ 45 ] ."
USP20 affects TRIM23
1 |
1 |

No evidence text available
USP20 affects TRAF2
| 1
| 1

sparser
"With these co-immunoprecipitation assays, we found that USP20 associated with the ubiquitin E3 ligases TRAF2, cIAP1, and two components of LUBAC xref : HOIL-1-interacting protein (HOIP, or RNF31) and SHARPIN (also known as SIPL1) ( xref )."
USP20 affects TNFalpha-mediated
| 1
TNFalpha-mediated binds USP20. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
USP20 affects TNFalpha-induced NF-kappaB activation regarding PKCzeta
| 1
USP20 activates TNFalpha-induced NF-kappaB activation regarding PKCzeta. 1 / 1
| 1

eidos
"Based on our data that USP20 is responsible for NF-kappaB-mediated cell survival through stabilizing p62 , we also suggest that USP20 depletion causes the blockade of TNFalpha-induced NF-kappaB activation regarding PKCzeta and thus may accelerate cell death pathways ."
USP20 affects TLR4-induced NF-kappaB
| 1
USP20 inhibits TLR4-induced NF-kappaB. 1 / 1
| 1

eidos
"Intriguingly , USP20 impedes TLR4-induced NF-kappaB activation by deubiquitinating TNF receptor-associated factor 6,147 whereas it inhibits IL-1R and TNF-triggered NF-kappaB activation by deubiquitinating receptor-interacting protein kinase 1.148 Consistent with the results of the in vitro experiments , USP20 decreased neointimal hyperplasia caused by carotid endothelial denudation in a transgenic mice model ."
USP20 affects Signaling
| 1
USP20 inhibits Signaling. 1 / 1
| 1

sparser
"USP20 Inhibits IL-1R-evoked Inflammatory Signaling in SMCs."
USP20 affects SYTL4
1 |
1 |

No evidence text available
USP20 affects SUN2
1 |
1 |

No evidence text available
USP20 affects SPRY2
1 |
1 |

No evidence text available
USP20 affects SNAI2
| 1
USP20 activates SNAI2. 1 / 1
| 1

reach
"Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2."

reach
"All these effects mediated by USP20 during cerebral I/R injury were confirmed in the cultured primary microglial cells and cortical neurons stimulated by oxygen-glucose deprivation and reoxygenation (OGD/R)."
USP20 affects RGPD5
1 |
1 |

No evidence text available
USP20 affects RFC4
1 |
1 |

No evidence text available
USP20 affects RFC3
1 |
1 |

No evidence text available
USP20 affects RFC2
1 |
1 |

No evidence text available
USP20 affects PTEN
| 1
| 1

sparser
"Mechanistically, we found that USP20 directly interacted with PTEN."
USP20 affects PSMD7
1 |
1 |

No evidence text available
USP20 affects PSMD6
1 |
1 |

No evidence text available
USP20 affects PSMD12
1 |
1 |

No evidence text available
USP20 affects PSMD11
1 |
1 |

No evidence text available
USP20 affects PRKCI
1 |
1 |

No evidence text available
USP20 affects PRKACA
1 |
1 |

No evidence text available
USP20 affects POLDIP3
1 |
1 |

No evidence text available
USP20 affects PLK1
1 |
1 |

No evidence text available
USP20 affects PLEKHA7
1 |
1 |

No evidence text available
USP20 affects PLEC
1 |
1 |

No evidence text available
USP20 affects PLA2G2A
1 |
1 |

No evidence text available
USP20 affects PKCzeta-RIPK1-p62
| 1
PKCzeta-RIPK1-p62 binds USP20. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
USP20 affects PJA1
1 |
1 |

No evidence text available
USP20 affects Neoplasms
| 1
| 1

reach
"USP20 can support progression of variety of tumors."
USP20 affects NUP153
1 |
1 |

No evidence text available
USP20 affects NFASC
| 1
USP20 activates NFASC. 1 / 1
| 1

reach
"Our findings reveal novel mechanisms regulating IL-1β-induced pro-inflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation and neointimal hyperplasia."
USP20 affects NF-kappaB-mediated target
| 1
USP20 activates NF-kappaB-mediated target. 1 / 1
| 1

eidos
"In addition , quantitative real-time RT-PCR analysis revealed that USP20 depletion decreases expression of NF-kappaB-mediated target genes such as BFL1 and cFLIP , which are related to cell survival , upon TNFalpha treatment ( Figure 3D ) ."
USP20 affects N4BP3
1 |
1 |

No evidence text available
USP20 affects Mice
| 1
USP20 inhibits Mice. 1 / 1
| 1

reach
"Ubiquitin-specific peptidase 20 (USP20) reduces NFκB activation triggered by pro-inflammatory stimuli, and USP20 activity attenuates atherosclerosis in mice."
USP20 affects MYO1C
1 |
1 |

No evidence text available
USP20 affects MYO1A
1 |
1 |

No evidence text available
USP20 affects MDFI
1 |
1 |

No evidence text available
USP20 affects MCAO-operated mice
| 1
USP20 inhibits MCAO-operated mice. 1 / 1
| 1

eidos
"Notably , suppressing PTEN with its specific inhibitor dramatically abolished the function of USP20 to ameliorate neuroinflammation and neuron death induced by OGD / R. Collectively , our results illustrated that USP20 could effectively mitigate the severity of cerebral ischemic stroke and improve behavior deficits in MCAO-operated mice , and identified the USP20 / PTEN axis as a promising therapeutic target for ischemic stroke treatment ."
USP20 affects MCAO-induced ischemic brain injury
| 1
USP20 activates MCAO-induced ischemic brain injury. 1 / 1
| 1

eidos
"We then found that promoting USP20 expression remarkably ameliorated MCAO-induced ischemic brain injury , along with significantly reduced infarct volume , neurological scores and brain water contents ."
USP20 affects MAP7D2
1 |
1 |

No evidence text available
USP20 affects MAP7D1
1 |
1 |

No evidence text available
USP20 affects MAGEA11
1 |
1 |

No evidence text available
USP20 affects LRRC59
1 |
1 |

No evidence text available
USP20 affects LMNA
1 |
1 |

No evidence text available
USP20 affects K48-linked deubiquitination STING
| 1
USP20 activates K48-linked deubiquitination STING. 1 / 1
| 1

eidos
"On the other hand , OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation , thus maintain the stability of STING and promote activation of STING pathway ( 87 ) ( 88 ) ( 89 ) ."
USP20 affects K48-
| 1
USP20 activates K48-. 1 / 1
| 1

reach
"The DUB USP20 mediates removal of both K48- and K63 linked polyubiquitin chains."
USP20 affects Interferon
| 1
| 1

reach
"XREF_BIBR This study showed that USP18 or USP20 deficiency significantly inhibited HSV-I or cytosolic DNA activation of both NF-kappaB and IRF3, and type-I IFN and proinflammatory cytokine expression."
USP20 affects Infections
| 1
| 1

reach
"The complementation of STING into Usp20 (-/-) cells remarkably restores HSV-1-triggered signaling and inhibits HSV-1 infection (92)."
USP20 affects INS
| 1
USP20 activates INS. 1 / 1
| 1

reach
"Genetic deletion or pharmacological inhibition of USP20 markedly decreases diet induced body weight gain, reduces lipid levels in the serum and liver, improves insulin sensitivity and increases energy expenditure."
USP20 affects IL1
| 1
USP20 activates IL1. 1 / 1
| 1

reach
"Phosphorylation of USP20 on Ser334 by IRAK-1 Promotes IL-1β-evoked Signaling in Vascular Smooth Muscle Cells and Vascular Inflammation."
USP20 affects IKBKG
| 1
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."
USP20 affects IFNs proinflammatory cytokines
| 1
USP20 activates IFNs proinflammatory cytokines. 1 / 1
| 1

eidos
"Mechanistically , USP20 is recruited by USP18 to deconjugate K48-linked ubiquitination chains from STING and thus promotes the stability of STING and the expression of type I IFNs and proinflammatory cytokines after DNA virus infection ( 91 ) ."
USP20 affects Hyperplasia
| 1
| 1

reach
"Our findings reveal novel mechanisms regulating IL-1β-induced pro-inflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation and neointimal hyperplasia."
USP20 affects HMGCR
| 1
Phosphorylated USP20 deubiquitinates HMGCR. 1 / 1
| 1

reach
"In contrast to ubiquitination, HMGCR is deubiquitinated by mTORC1-phosphorylated USP20, which preferentially hydrolyzes K48 and K63 linkages, and stabilized HMGCR increases cholesterol synthesis in the feeding state (Lu et al., 2020)."
USP20 affects HIF-1alpha target
| 1
USP20 activates HIF-1alpha target. 1 / 1
| 1

eidos
"As a consequence of this interaction , USP20 induces the expression of HIF-1alpha target genes , such as vascular endothelial growth factor ( VEGF ) ."
USP20 affects HIF-1alpha [
| 1
USP20 binds HIF-1alpha [. 1 / 1
| 1

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."
USP20 affects HIF)-1alpha
| 1
USP20 deubiquitinates HIF)-1alpha. 1 / 1
| 1

reach
"Indeed, USP20 deubiquitinates and stabilizes its associated protein, hypoxia inducible factor (HIF)-1alpha, resulting in the promotion of cancer cell proliferation, invasion, and metastasis [XREF_BIBR, XREF_BIBR]."
USP20 affects HERC2P2
1 |
1 |

No evidence text available

reach
"USP20 promotes genome stability and suppresses xenograft tumor growth."
USP20 affects GTF2IRD1
1 |

No evidence text available
USP20 affects GPT2
1 |
1 |

No evidence text available
USP20 affects GCGR
| 1
USP20 deubiquitinates GCGR. 1 / 1
| 1

reach
"USP20, which regulates β - and β -ARs (18, 19), also does not deubiquitinate GCGR, because in cells with USP20 knockdown, the extent of glucagon-induced deubiquitination is identical to cells with control knockdown (Fig. S4, C and D)."
USP20 affects FECH
1 |
1 |

No evidence text available
USP20 affects FATE1
1 |
1 |

No evidence text available
USP20 affects EIF3E
1 |
1 |

No evidence text available
USP20 affects EIF3C
1 |
1 |

No evidence text available
USP20 affects ECI2
1 |
1 |

No evidence text available
| 1

reach
"The association of USP20 with its substrates triggers deubiquitinase activity; this association is regulated by phosphorylation of USP20 on Ser334 (mouse) or Ser333 (human)."

sparser
"These results establish the important role of USP20 phosphorylation in ATR-dependent DDR."
USP20 affects DDX58
| 1
USP20 activates DDX58. 1 / 1
| 1

reach
"For example, USP17 and USP20 were found to target RIG-I and TRAF6 respectively, thereby functioning as novel regulators of antiviral innate immune responses [XREF_BIBR, XREF_BIBR]."
USP20 affects Cellular Antiviral Responses
| 1
USP20 activates Cellular Antiviral Responses. 1 / 1
| 1

reach
"USP20 Promotes Cellular Antiviral Responses via Deconjugating K48 Linked Ubiquitination of MITA."
| 1
| 1

reach
"Furthermore, reconstitution of WT USP20 but not USP20-4 mut rescued the cell viability after UV treatment and restored UV or HU induced intra-S-phase checkpoint."
USP20 affects CTNNB1
| 1
USP20 leads to the deubiquitination of CTNNB1. 1 / 1
| 1

reach
"Moreover, knockdown of USP20 increases beta-catenin polyubiquitination, which enhances beta-catenin turnover and cell sensitivity to chemotherapy."
USP20 affects CRAMP1
1 |
1 |

No evidence text available
USP20 affects CLAPSIN
| 1
USP20 binds CLAPSIN. 1 / 1
| 1

reach
"We then wanted to determine the functional significance of the interaction between USP20 and CLAPSIN."
USP20 affects CI
| 1
| 1

sparser
"After plasmids encoding Flag-p62 and HA-ubiquitin (HA-Ubi) were co-transfected into HeLa cells, together with wild-type (WT) USP20 or catalytically inactive mutant USP20 (USP20-CI), immunoprecipitation and immunoblot assays were performed."
USP20 affects CCDC36
1 |
1 |

No evidence text available
USP20 affects CALM1
1 |
1 |

No evidence text available
| 1

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."
USP20 affects BIRC3
| 1
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."
USP20 affects BEND7
1 |
1 |

No evidence text available
USP20 affects BEND5
1 |
1 |

No evidence text available
USP20 affects BCAM
| 1
USP20 activates BCAM. 1 / 1
| 1

reach
"Lu et al. demonstrated the post-prandial increase in insulin and the glucose concentration stimulates mTORC1 to phosphorylate the deubiquitylase ubiquitin-specific peptidase 20 (USP20) at S132 and S134, which is recruited to the HMGCR complex and antagonizes its degradation (Lu et al., 2020)."
USP20 affects Ala-Pro
| 1
USP20 activates Ala-Pro. 1 / 1
| 1

reach
"In contrast, the overexpression of Usp26 decreased the number of AP + colonies, while overexpression of Usp20 increased the number of AP + colonies."
USP20 affects ATR-Chk1 [
| 1
USP20 activates ATR-Chk1 [. 1 / 1
| 1

reach
"USP20 serves as a novel regulator of ATR dependent DNA damage checkpoint signaling through the deubiquitination and stabilization of claspin and enhances the activation of ATR-Chk1 [XREF_BIBR]."
USP20 affects ATR-CLASPIN-CHK1
| 1
USP20 activates ATR-CLASPIN-CHK1. 1 / 1
| 1

reach
"Our results demonstrated that USP20 positively regulates the ATR-CLASPIN-CHK1 signaling during replication stress."
USP20 affects ATR
1 |
1 |

No evidence text available
USP20 affects ATP6AP2
1 |
1 |

No evidence text available
USP20 affects ATAD3B
1 |
1 |

No evidence text available
USP20 affects ARs
| 1
USP20 binds USP33 and ARs. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP20 affects ALAS1
1 |
1 |

No evidence text available
USP20 affects ADRB1
| 1
USP20 inhibits ADRB1. 1 / 1
| 1

reach
"For example, ubiquitin carboxyl-terminal hydrolase 20 (USP20) knockout reduces beta1-adrenergic receptor (beta1AR)-induced cardiac contractility and relaxation."
USP20 affects ACADM
1 |
1 |

No evidence text available
USP15 affects USP20
1 |
1 |

No evidence text available
UCHL3 affects USP20
1 |
1 |

No evidence text available
TRIM23 affects USP20
1 |
1 |

No evidence text available
TRAF2 affects USP20
| 1
| 1

sparser
"With these co-immunoprecipitation assays, we found that USP20 associated with the ubiquitin E3 ligases TRAF2, cIAP1, and two components of LUBAC xref : HOIL-1-interacting protein (HOIP, or RNF31) and SHARPIN (also known as SIPL1) ( xref )."
TNFalpha-mediated affects USP20
| 1
TNFalpha-mediated binds USP20. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
TNFRSF1A affects IKBKG
| 1
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."
TNF affects USP20
| 1
| 1

sparser
"Co-immunoprecipitation experiments revealed that USP20 associates with several components of the TNF receptor-1 signaling pathway including receptor-interacting protein kinase 1 (RIPK1), a critical checkpoint in TNF-induced NFκB activation and inflammation."
TINCR affects USP20
| 1
TINCR activates USP20. 1 / 1
| 1

reach
"Furthermore, in the cytoplasm, TINCR potentially acts as a molecular sponge of miR-199a-5p and upregulates the stability of USP20 mRNA through a competing endogenous RNA (ceRNA) regulatory mechanism, thus promoting PD-L1 expression by decreasing its ubiquitination level."
SYTL4 affects USP20
1 |
1 |

No evidence text available
SUN2 affects USP20
1 |
1 |

No evidence text available
SPRY2 affects USP20
1 |
1 |

No evidence text available
RIPK1 affects USP20
| 1
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
RGPD5 affects USP20
1 |
1 |

No evidence text available
RFC4 affects USP20
1 |
1 |

No evidence text available
RFC3 affects USP20
1 |
1 |

No evidence text available
RFC2 affects USP20
1 |
1 |

No evidence text available
PSMD7 affects USP20
1 |
1 |

No evidence text available
PSMD6 affects USP20
1 |
1 |

No evidence text available
PSMD12 affects USP20
1 |
1 |

No evidence text available
PSMD11 affects USP20
1 |
1 |

No evidence text available
PRKCI affects USP20
1 |
1 |

No evidence text available
POLDIP3 affects USP20
1 |
1 |

No evidence text available
PLK1 affects USP20
1 |
1 |

No evidence text available
PLEKHA7 affects USP20
1 |
1 |

No evidence text available
PLEC affects USP20
1 |
1 |

No evidence text available
PLA2G2A affects USP20
1 |
1 |

No evidence text available
PKCzeta-RIPK1-p62 affects USP20
| 1
PKCzeta-RIPK1-p62 binds USP20. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
PJA1 affects USP20
1 |
1 |

No evidence text available
P22077 affects USP20
| 1
P22077 inhibits USP20. 1 / 1
| 1

eidos
"P22077 inhibited only USP7 , USP9x , USP10 and USP20 appreciably at 1 muM ( USP47 was not tested ) ."
Niclosamide affects USP20
1 |
Niclosamide increases the amount of USP20. 1 / 1
1 |

No evidence text available
NUP153 affects USP20
1 |
1 |

No evidence text available
NFkappaB affects USP20
| 1
NFkappaB binds USP20. 1 / 1
| 1

isi
"USP20 specifically binds to p62 and acts as a positive regulator for NF-kappaB activation by TNFalpha through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival."
NFKBIA affects RIPK1
| 1
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
N4BP3 affects USP20
1 |
1 |

No evidence text available

reach
"Our experiments showed that hVDU2 protein was extremely stable in the group without co-transfection of pVHL during cycloheximide treatment, and MG132 did not significantly increase the level of VDU2 p[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
MYO1C affects USP20
1 |
1 |

No evidence text available
MYO1A affects USP20
1 |
1 |

No evidence text available
MDFI affects USP20
1 |
1 |

No evidence text available
MCL1 affects USP20
| 1
| 1

sparser
"Overall, our findings indicate that the USP20-MCL1 axis might play a key role in the apoptotic signaling pathway."
MAP7D2 affects USP20
1 |
1 |

No evidence text available
MAP7D1 affects USP20
1 |
1 |

No evidence text available
MAGEA11 affects USP20
1 |
1 |

No evidence text available
LRRC59 affects USP20
1 |
1 |

No evidence text available
LMNA affects USP20
1 |
1 |

No evidence text available
IL1B affects USP20
| 1
IL1B phosphorylates USP20 on S333. 1 / 1
| 1

sparser
"Using in vitro phosphorylation with purified IRAK1 and siRNA-mediated gene silencing of IRAK1 in SMCs, we identified IRAK1 as a novel kinase for IL-1β-induced USP20 Ser334 phosphorylation."
IKBKG affects USP20
| 1
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."
HIF-1alpha [ affects USP20
| 1
USP20 binds HIF-1alpha [. 1 / 1
| 1

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."
HERC2P2 affects USP20
1 |
1 |

No evidence text available
HERC2 affects CLSPN
| 1
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
GTF2IRD1 affects USP20
1 |

No evidence text available
GSK2643943A affects USP20
| 1
GSK2643943A inhibits USP20. 1 / 1
| 1

eidos
"For example , 8-mercapto-N - ( ( tetrahydro-3-furanyl ) methyl ) -4 - quinoline carboxamide , LND-57444 , VLX1570 , ML323 , ( ADC-01 , ADC-03 , HBX41108 , HBX19818 , P5091 , P22077 ) , 9 - ( ethoxyimino ) -9 H-indeno ( 1,2 - b ) pyrazine-2 ,3 - dicarbonitrile , WP1130 , Mitoxantrone and GSK2643943A are able to inhibit PSMD14 , UCHL1 , UCHL5 and USP14 , USP1 , USP7 , USP8 , USP9X , USP11 and USP20 , respectively ."
GPT2 affects USP20
1 |
1 |

No evidence text available
FECH affects USP20
1 |
1 |

No evidence text available
FATE1 affects USP20
1 |
1 |

No evidence text available
EIF3E affects USP20
1 |
1 |

No evidence text available
EIF3C affects USP20
1 |
1 |

No evidence text available
ECI2 affects USP20
1 |
1 |

No evidence text available
| 1
E3_Ub_ligase ubiquitinates USP20. 1 / 1
| 1

reach
"To test whether hVDU2 can be ubiquitinated by pVHL E3 ubiquitin ligase complex, we performed ubiquitination assay in vitro and in vivo."
CRAMP1 affects USP20
1 |
1 |

No evidence text available
CNTN2 affects USP20
| 1
| 1

reach
"Tax-1 interacts with NRP and optineurin and TAX1BP1, and with the ubiquitin specific peptidase USP20."
CLSPN affects HERC2, and USP20
| 1
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
CLAPSIN affects USP20
| 1
USP20 binds CLAPSIN. 1 / 1
| 1

reach
"We then wanted to determine the functional significance of the interaction between USP20 and CLAPSIN."
CI affects USP20
| 1
| 1

sparser
"After plasmids encoding Flag-p62 and HA-ubiquitin (HA-Ubi) were co-transfected into HeLa cells, together with wild-type (WT) USP20 or catalytically inactive mutant USP20 (USP20-CI), immunoprecipitation and immunoblot assays were performed."
CHEK1 affects USP20
| 1
CHEK1 activates USP20. 1 / 1
| 1

sparser
"We found that overexpression of FLAG-CLASPIN corrected the delay of USP20 depletion-induced CHK1 activation in response to HU treatment (Figure xref )."
CCP110 affects USP20, and USP33
| 1
| 1

sparser
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
CCDC36 affects USP20
1 |
1 |

No evidence text available
CALM1 affects USP20
1 |
1 |

No evidence text available
BIRC3 affects USP20
| 1
| 1

sparser
"Of these USP20-associating proteins, only β-arrestin2 has previously been described. xref In contrast, we could not detect specific association of USP20 with TNFR1, cIAP2, or IKKγ ( xref )."
BEND7 affects USP20
1 |
1 |

No evidence text available
BEND5 affects USP20
1 |
1 |

No evidence text available
Arrestins affects USP20
| 1
Arrestins deubiquitinates USP20. 1 / 1
| 1

reach
"Arrestins also recruit deubiquitinating enzymes USP33 and USP20, important for recycling and resensitisation to the beta2-adrenergic receptor."
AZM551248 affects USP20
1 |
AZM551248 decreases the amount of USP20. 1 / 1
1 |

No evidence text available
ATP6AP2 affects USP20
1 |
1 |

No evidence text available
ATAD3B affects USP20
1 |
1 |

No evidence text available
ARs affects USP33
| 1
USP20 binds USP33 and ARs. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
ARRB2 affects RIPK1
| 1
| 1

sparser
"In addition, USP20 associated with RIPK1, IκBα, and β-arrestin2 ( xref )."
AR affects USP33
| 1
USP20 binds USP33 and AR. 1 / 1
| 1

sparser
"Because carvedilol enhanced β 2 AR ubiquitination and degradation independently of the known β 2 AR E3 ligase Nedd4, we reasoned that carvedilol might diminish β 2 AR deubiquitination by reducing the association of the β 2 AR with USP20 and/or USP33."
ALAS1 affects USP20
1 |
1 |

No evidence text available
ACADM affects USP20
1 |
1 |

No evidence text available
2-hydroxypropanoic acid decreases the amount of USP20. 1 / 1
1 |

No evidence text available
17alpha-ethynylestradiol decreases the amount of USP20. 1 / 1
1 |

No evidence text available
| 1

reach
"VDU1, but not VDU2, is markedly increased in brown adipocytes by norepinephrine or cold exposure, further amplifying the increase in D2 activity that results from catecholamine stimulated de novo synthesis."
1 |
(+)-JQ1 compound increases the amount of USP20. 1 / 1
1 |

No evidence text available