IndraLab

Statements

HERC2 ubiquitinates USP20. 10 / 11
2 1 | 4 3
signor
"USP20 is regulated by HERC2 following DNA damage or replication stress.The HECT domain of WT HERC but not the catalytic inactive mutant (CA) ubiquitinated USP20 in vitro (Figure ​(Figure4G).4G). Taken together, these results suggested that HERC2 functions as an E3 ligase of USP20 and negatively regulates USP20 in unstressed cells."
25355518
reach
"To determine whether HERC2 directly ubiquitinates USP20, we performed an in vitro ubiquitination assay."
25355518
signor
"HERC2 promotes USP20 degradation.|Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."
25326330
reach
"On the other hand, when cells recover from DNA damage, HERC2 mediates USP20 ubiquitination, turning off the DDR signaling."
25355518
sparser
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination-mediated proteasomal degradation of USP20, regulating the status of K48-linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."
25326330
reach
"This phosphorylation event could trigger the dissociation of the HERC2-USP20 interaction, thereby preventing the ubiquitylation of USP20 by HERC2 and subsequently stabilizing USP20 protein levels."
38570483
reach
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."
25326330
ubibrowser
"UbiNet 2.0"
25355518
sparser
"To determine whether HERC2 directly ubiquitinates USP20, we performed an in vitro ubiquitination assay."
25355518
sparser
"This phosphorylation event could trigger the dissociation of the HERC2-USP20 interaction, thereby preventing the ubiquitylation of USP20 by HERC2 and subsequently stabilizing USP20 protein levels."
38570483