IndraLab

Statements

ATR phosphorylates USP20. 40 / 41
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sparser
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU-induced replication stress."
25326330
sparser
"Upon replication stress, ATR phosphorylates USP20 promoting its dissociation from the complex."
28942358
rlimsp
"USP20 itself is phosphorylated by ATR, resulting in its stabilization and further activating ATR-Chk1 signaling following replication stress."
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rlimsp
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU-induced replication stress."
25326330
reach
"Upon replication stress, ATR phosphorylates USP20 promoting its dissociation from the complex."
28942358
sparser
"Upon DNA damage, ATR phosphorylates USP20, a deubiquitinating enzyme which is a degradation target of HERC2 ( xref A)."
32580485
reach
"Inhibition of ATR by BAY1895344 or siRNA markedly decreased the phosphorylation of USP20 as well as its protein levels (Figures 6C and S7C)."
38124786
reach
"We generated the USP20 mutant vector (USP20‐S132A/S368A) by replacing serine with alanine, and our results indicated that ATR phosphorylates wild‐type USP20 (USP20‐WT) but not USP20‐S132A/S368A (Figure 6D)."
38124786
reach
"As ATR phosphorylates USP20 and increases its protein levels, we further examined the effects of DNA‐damage induced ATR activation on ferroptosis."
38124786
reach
"IP assay indicated that USP20 could be phosphorylated by ATR."
38124786
reach
"In our present study, we found that inhibition of ATR significantly reduced the phosphorylation of USP20 and further increased the polyubiquitination of USP20, resulting its destabilization."
38124786
sparser
"Besides, ATR phosphorylates and also stabilizes USP20 by unbinding it from HERC2."
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reach
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU induced replication stress."
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rlimsp
"We next set out to determine the ATR phosphorylation sites of USP20."
25355518
rlimsp
"USP20 is phosphorylated by ATR, resulting in disassociation of the E3 ubiquitin ligase HERC2 from USP20 and USP20 stabilization."
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signor
"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation"
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rlimsp
"These results suggest that ATR-mediated phosphorylation of USP20 is required for USP20 stabilization through inducing disassociation of HERC2 and USP20."
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signor
"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization.|USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."
25355518
sparser
"USP20 is phosphorylated by ATR, resulting in disassociation of the E3 ubiquitin ligase HERC2 from USP20 and USP20 stabilization."
25355518
reach
"USP20 is phosphorylated by ATR, resulting in disassociation of the E3 ubiquitin ligase HERC2 from USP20 and USP20 stabilization."
25355518
rlimsp
"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."
25355518
reach
"USP20 itself is phosphorylated by ATR, resulting in its stabilization and further activating ATR-Chk1 signaling following replication stress."
25355518
sparser
"ATR phosphorylates and stabilizes USP20."
38124786
sparser
"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."
25355518
sparser
"We then performed point mutation and IP to verify that ATR phosphorylates S132 and S368 sites of USP20."
38124786
sparser
"We generated the USP20 mutant vector (USP20‐S132A/S368A) by replacing serine with alanine, and our results indicated that ATR phosphorylates wild‐type USP20 (USP20‐WT) but not USP20‐S132A/S368A (Figure  xref )."
38124786
reach
"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."
25355518
rlimsp
"ATR-mediated phosphorylation of USP20 promotes its dissociation from HERC2."
25326330
sparser
"As ATR phosphorylates USP20 and increases its protein levels, we further examined the effects of DNA‐damage induced ATR activation on ferroptosis."
38124786
reach
"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization."
25355518
reach
"Mechanistically, the phosphorylation of USP20 by ATR disrupts the interaction between HERC2 and USP20 and is responsible for USP20 upregulation following the DDR."
25355518
reach
"USP20 phosphorylation by ATR does not affect its E3 ligase activity per se, suggesting that USP20 levels, but not catalytic activity, are subjected to ATR regulation."
25355518
sparser
"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization."
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rlimsp
"(B and C) USP20 was phosphorylated by ATR in response to HU treatment."
25326330
sparser
"IP assay indicated that USP20 could be phosphorylated by ATR."
38124786
rlimsp
"USP20 phosphorylation by ATR does not affect its E3 ligase activity per se, suggesting that USP20 levels, but not catalytic activity, are subjected to ATR regulation."
25355518
rlimsp
"Mechanistically, the phosphorylation of USP20 by ATR disrupts the interaction between HERC2 and USP20 and is responsible for USP20 upregulation following the DDR."
25355518
sparser
"Mechanistically, the phosphorylation of USP20 by ATR disrupts the interaction between HERC2 and USP20 and is responsible for USP20 upregulation following the DDR."
25355518
rlimsp
"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization."
25355518
sparser
"USP20 phosphorylation by ATR does not affect its E3 ligase activity per se , suggesting that USP20 levels, but not catalytic activity, are subjected to ATR regulation."
25355518