IndraLab

Statements


USP20 is modified
42 | 2 158 16
USP20 is phosphorylated.
42 | 147 16
USP20 is phosphorylated. 10 / 87
| 73 14

rlimsp
"Upon replication stress, ATR-mediated phosphorylation of USP20 promotes dissociation of HERC2 from USP20, stabilizes USP20 and its association with CLASPIN, thus increasing CALSPIN stability and ensuring CHK1 activation."

rlimsp
"Currently, we do not know how USP20 phosphorylation affects its binding to HERC2."

rlimsp
"One is that USP20 phosphorylation itself affects the binding between USP20 and HERC2."

sparser
"Subsequently, it was also found that long-term high-sugar and high-fat diet induced increased phosphorylation of USP20, stabilized HMGCR protein and increased cholesterol, which caused metabolic diseases."

sparser
"Subsequently, the upregulation of postprandial glucose and insulin levels promotes the phosphorylation of USP20 ( xref )."

sparser
"ATR-mediated phosphorylation of USP20 promotes its dissociation from HERC2."

sparser
"We then wanted to determine the biological function of ATR-mediated phosphorylation of USP20."

rlimsp
"ATR-mediated phosphorylation of USP20 promotes its dissociation from HERC2."

rlimsp
"Our findings reveal a dynamic regulation of USP20 by site-specific phosphorylation as well as the interdependence of signal transduction and trafficking pathways in balancing adrenergic stimulation and maintaining cellular homeostasis."

sparser
"We recently showed that USP20 phosphorylation is significantly elevated in SMCs from human atherosclerotic segments compared with SMCs from nonatherosclerotic segments xref ."
USP20 is phosphorylated on S333. 10 / 50
| 50

sparser
"Taken together, these data suggest that phosphorylation of USP20 on Ser334 augments SMC proliferation and migration, particularly to agonists distinct from PDGF, like IL-1β."

sparser
"Preventing USP20 phosphorylation on Ser334 augments association with and deubiquitination of TRAF6."

sparser
"To understand mechanisms by which phosphorylation of USP20 on Ser334 may regulate SMC and vascular inflammation, we first asked whether USP20-S334A retains a WT level of ubiquitin-binding activity—because substrate binding is the rate-limiting step in DUB activity ( xref )."

sparser
"Thus, it seems that preventing USP20 phosphorylation on Ser334 augments the association of USP20 with its intracellular substrate TRAF6, even though it has no effect on the association of USP20 with the idealized substrate Ub-VME ( xref , A and B )."

sparser
"Recent studies have shown that β 2 AR activation triggers site-specific phosphorylation of USP20 by the cAMP-activated protein kinase A (PKA) at serine 333 of USP20 [ xref ]."

sparser
"Next, we performed the proteome profiler assay using the proteins extracted from sprouted aortic rings of USP20-S334D and congenic WT ( xref , xref ) in order to identify specific angiogenic factors regulated by S334 phosphorylation of USP20."

sparser
"Reversible phosphorylation of USP20 at S333 could be a critical factor that determines the rate of down-regulation of β 2 ARs via autophagosomes during catecholamine stress [ xref ]."

sparser
"Residing within the motif RMKDRKF S 334 WGQQRTN, the Ser334 of USP20 is phosphorylated by PKAα—even though the amino acid sequence slightly diverges from the canonical RRxS PKA motif ( xref , xref )."

sparser
"Phosphorylation of human USP20 on Ser333 in the absence of purified kinase was attributed to the activity of one or more kinases in the COS-7 cells."

sparser
"We conclude that USP20 expression inversely correlates with the extent of angiogenesis, and that inhibiting USP20 Ser334 phosphorylation could be a useful strategy to constrain inflammation-driven angiogenesis under pathological circumstances, like cancer and atherosclerosis."
USP20 is phosphorylated on S132. 10 / 22
10 | 12

sparser
"Phosphorylation of USP20 at Ser132 and Ser368 residues was detected through MS analysis (Figure xref )."

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sparser
"And the phosphorylation of USP20 at Ser132 and Ser368 requires the activation of ATR induced by DNA damage xref ."

sparser
"USP20 phosphorylation at Ser132 and Ser368 enhanced its stability and thus conferred OXA and ferroptosis resistance of HCC cells (Figure  xref )."

sparser
"Most importantly, DNA damage‐induced ATR activation is required for Ser132 and Ser368 phosphorylation of USP20."

sparser
"USP20 phosphorylation at Ser132 and Ser368 enhances its stability and thus confers OXA and ferroptosis resistance of HCC cells."

sparser
"Postprandial increases in insulin and glucose concentrations synergistically activated mTORC1 and phosphorylate USP20 at S132 and S134."

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USP20 is phosphorylated on S134. 10 / 15
10 | 5

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sparser
"Postprandial increases in insulin and glucose levels stimulate mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is recruited to the HMGCR complex and counteracts its degradation."

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sparser
"Specifically, feeding mice a high-sucrose, low-fat diet stimulated mTORC1 to phosphorylate USP20 at S132 and S134."

sparser
"Elevated postprandial glucose and insulin levels stimulate mTORC1 to phosphorylate S132 and S134 in USP20."

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USP20 is phosphorylated on S368. 7 / 7
| 7

sparser
"Phosphorylation of USP20 at Ser132 and Ser368 residues was detected through MS analysis (Figure xref )."

sparser
"And the phosphorylation of USP20 at Ser132 and Ser368 requires the activation of ATR induced by DNA damage xref ."

sparser
"USP20 phosphorylation at Ser132 and Ser368 enhanced its stability and thus conferred OXA and ferroptosis resistance of HCC cells (Figure  xref )."

sparser
"Most importantly, DNA damage‐induced ATR activation was required for Ser132 and Ser368 phosphorylation of USP20."

sparser
"Most importantly, DNA damage‐induced ATR activation is required for Ser132 and Ser368 phosphorylation of USP20."

sparser
"USP20 phosphorylation at Ser132 and Ser368 enhances its stability and thus confers OXA and ferroptosis resistance of HCC cells."

sparser
"USP20 phosphorylation at Ser132 and Ser368 enhanced its stability and thus conferred OXA and ferroptosis resistance of HCC cells."
USP20 is phosphorylated on S373. 4 / 4
4 |

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USP20 is phosphorylated on S413. 4 / 4
4 |

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USP20 is phosphorylated on S407. 4 / 4
4 |

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USP20 is phosphorylated on T377. 2 / 2
2 |

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USP20 is phosphorylated on T258. 2 / 2
2 |

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USP20 is phosphorylated on S372. 2 / 2
2 |

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USP20 is phosphorylated on S406. 2 / 2
2 |

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USP20 is phosphorylated on S263. 2 / 2
2 |

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USP20 is phosphorylated. 1 / 1
| 1

rlimsp
"Although we have not tested the FFAT-like motifs in USP20 and RMD3, our analysis suggests a possible mechanism to regulate the function both of USP20 in receptor recycling [56] and of RMD3 in apoptosis [55]: by phosphorylation of their FFAT-like motifs."
USP20 is phosphorylated on S333. 1 / 1
| 1

rlimsp
"In this process, phosphorylation of USP20 at serine residue 333 is required for its activity providing an additional level of regulation [148]."
USP20 is ubiquitinated.
| 2 10
USP20 is ubiquitinated. 10 / 12
| 2 10

sparser
"To confirm this, we examined the ubiquitination of USP20 in cells depleted of HERC2."

sparser
"Downregulation of HERC2 resulted in significant decrease in USP20 ubiquitination in vivo (Figure xref )."

sparser
"The HECT domain of WT HERC but not the catalytic inactive mutant (CA) ubiquitinated USP20 in vitro (Figure xref )."

sparser
"ATR inhibition results in increased ubiquitination of wild-type USP20."

sparser
"As USP20 ubiquitination decreases following replication stress, we next studied whether the USP20-HERC2 interaction is subjected to regulation by the DDR pathway."

reach
"Although pVHL functions as a master control for HIF-1alpha stabilization, as pVHL-E3 ligase mediates the ubiquitination of both HIF-1alpha and VDU2, the balance between the pVHL mediated ubiquitination and VDU2 mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."

sparser
"On the other hand, when cells recover from DNA damage, HERC2 mediates USP20 ubiquitination, turning off the DDR signaling."

sparser
"Additionally, USP20 could be ubiquitinated in a pVHL-dependent manner, inducing itself rapid degradation ( xref )."

reach
"The balance between the VHL mediated ubiquitination and VDU2 mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."

sparser
"Although pVHL functions as a master control for HIF-1alpha stabilization, as pVHL-E3 ligase mediates the ubiquitination of both HIF-1alpha and VDU2, the balance between the pVHL-mediated ubiquitination and VDU2-mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."
USP20 is dephosphorylated.
| 1
USP20 is dephosphorylated. 1 / 1
| 1

sparser
"Only dephosphorylated USP20 shows robust activity for deubiquitinating the β 2 AR."
HERC2 affects USP20
4 8 1 | 26 29
HERC2 binds USP20.
8 | 15 26
8 | 15 25

sparser
"Moreover, we show that p38 acts as a modulator of this pathway, since p38 activation disrupts HERC2-USP20 interaction, leading to increased USP20 and LC3-II protein levels."

reach
"In unstressed cells, HERC2 binds USP20 to maintain a low basal level of USP20 in cells."

reach
"During cell recovery from genotoxic stress, USP20 binds to HERC2 again and gets degraded."

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sparser
"Protein interaction of HERC2 with USP20 is regulated by p38 activation."

reach
"Chk1 activation is indirectly regulated via modulation of Claspin stability by ubiquitination and deubiquitination events, involving the HERC2 and USP20 complex [49,50]."

sparser
"To delve deeper into the molecular mechanisms underpinning HERC2-dependent autophagy regulation, we sought to determine whether HERC2 and USP20 proteins interact."

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sparser
"The results confirmed the interaction between HERC2 and USP20 proteins."

sparser
"Then, we hypothesized that p38 activity might modulate the interaction between HERC2 and USP20."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
HERC2 ubiquitinates USP20.
2 1 | 4 3
HERC2 ubiquitinates USP20. 10 / 11
2 1 | 4 3

"USP20 is regulated by HERC2 following DNA damage or replication stress.The HECT domain of WT HERC but not the catalytic inactive mutant (CA) ubiquitinated USP20 in vitro (Figure ​(Figure4G).4G). Taken together, these results suggested that HERC2 functions as an E3 ligase of USP20 and negatively regulates USP20 in unstressed cells."

reach
"To determine whether HERC2 directly ubiquitinates USP20, we performed an in vitro ubiquitination assay."

"HERC2 promotes USP20 degradation.|Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

reach
"On the other hand, when cells recover from DNA damage, HERC2 mediates USP20 ubiquitination, turning off the DDR signaling."

sparser
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination-mediated proteasomal degradation of USP20, regulating the status of K48-linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

reach
"This phosphorylation event could trigger the dissociation of the HERC2-USP20 interaction, thereby preventing the ubiquitylation of USP20 by HERC2 and subsequently stabilizing USP20 protein levels."

reach
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

sparser
"To determine whether HERC2 directly ubiquitinates USP20, we performed an in vitro ubiquitination assay."

sparser
"This phosphorylation event could trigger the dissociation of the HERC2-USP20 interaction, thereby preventing the ubiquitylation of USP20 by HERC2 and subsequently stabilizing USP20 protein levels."
HERC2 inhibits USP20.
| 5
HERC2 inhibits USP20. 5 / 5
| 5

reach
"HERC2 promotes USP20 degradation."

reach
"Taken together, these results suggested that HERC2 functions as an E3 ligase of USP20 and negatively regulates USP20 in unstressed cells."

reach
"In HERC2 depleted cells, USP20 and its substrates Claspin were significantly upregulated and the phosphorylation of CHK1 is more sustained, even at later time points."

reach
"Here we found that HERC2 antagonized USP20 function and negatively regulated the replication stress signaling pathway."

reach
"Mechanistically, HERC2 promotes proteasomal degradation of USP20 (a deubiquitinase that catalyses deubiquitination of K48-linked ubiquitinated substrates) and thereby facilitates proteasomal degradati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
HERC2 decreases the amount of USP20.
2 | 1
HERC2 decreases the amount of USP20. 3 / 4
2 | 1

"USP20 is regulated by HERC2 following DNA damage or replication stress.The HECT domain of WT HERC but not the catalytic inactive mutant (CA) ubiquitinated USP20 in vitro (Figure ​(Figure4G).4G). Taken together, these results suggested that HERC2 functions as an E3 ligase of USP20 and negatively regulates USP20 in unstressed cells."

reach
"Mechanistically, we corroborate an interaction between HERC2 and the deubiquitylating enzyme USP20; and demonstrate that HERC2 deficiency leads to increased USP20 protein levels."

"HERC2 promotes USP20 degradation.|Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."
HERC2 activates USP20.
| 1
HERC2 activates USP20. 1 / 3
| 1

reach
"On the other hand, HERC2 has been shown to modulate the stability of USP20 by promoting its ubiquitylation-mediated proteasomal degradation [23, 24]."
ATR affects USP20
3 1 | 19 18 11
ATR phosphorylates USP20.
2 | 17 16 11
ATR phosphorylates USP20. 10 / 41
2 | 13 14 11

sparser
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU-induced replication stress."

sparser
"Upon replication stress, ATR phosphorylates USP20 promoting its dissociation from the complex."

rlimsp
"USP20 itself is phosphorylated by ATR, resulting in its stabilization and further activating ATR-Chk1 signaling following replication stress."

rlimsp
"Taken together, these data demonstrated that USP20 is phosphorylated by ATR in response to HU-induced replication stress."

reach
"Upon replication stress, ATR phosphorylates USP20 promoting its dissociation from the complex."

sparser
"Upon DNA damage, ATR phosphorylates USP20, a deubiquitinating enzyme which is a degradation target of HERC2 ( xref A)."

reach
"Inhibition of ATR by BAY1895344 or siRNA markedly decreased the phosphorylation of USP20 as well as its protein levels (Figures 6C and S7C)."

reach
"We generated the USP20 mutant vector (USP20‐S132A/S368A) by replacing serine with alanine, and our results indicated that ATR phosphorylates wild‐type USP20 (USP20‐WT) but not USP20‐S132A/S368A (Figure 6D)."

reach
"As ATR phosphorylates USP20 and increases its protein levels, we further examined the effects of DNA‐damage induced ATR activation on ferroptosis."

reach
"IP assay indicated that USP20 could be phosphorylated by ATR."
ATR phosphorylates USP20 on S368. 3 / 3
| 2 1

reach
"We then performed point mutation and IP to verify that ATR phosphorylates S132 and S368 sites of USP20."

sparser
"OXA induced DNA damage activates ATR, which phosphorylates USP20 at Ser132 and Ser368."

reach
"Most importantly, DNA damage-induced ATR activation was required for Ser132 and Ser368 phosphorylation of USP20."
ATR phosphorylates USP20 on S132. 3 / 3
| 2 1

sparser
"OXA induced DNA damage activates ATR, which phosphorylates USP20 at Ser132 and Ser368."

reach
"We then performed point mutation and IP to verify that ATR phosphorylates S132 and S368 sites of USP20."

reach
"Most importantly, DNA damage-induced ATR activation was required for Ser132 and Ser368 phosphorylation of USP20."
ATR binds USP20.
1 | 1 2
1 | 1 2

sparser
"We further identified the replication stress signal, ATR, which interacts with USP20."

sparser
"We conducted Co‐IP analysis in Huh‐7 cell lines and observed the interaction between ATR and USP20 (Figure  xref )."

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reach
"We conducted Co‐IP analysis in Huh‐7 cell lines and observed the interaction between ATR and USP20 (Figure 6A)."
ATR inhibits USP20.
1 |
ATR inhibits USP20. 1 / 2
1 |

"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization.|USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."
ATR ubiquitinates USP20.
| 1
ATR leads to the ubiquitination of USP20. 1 / 1
| 1

reach
"In our present study, we found that inhibition of ATR significantly reduced the phosphorylation of USP20 and further increased the polyubiquitination of USP20, resulting its destabilization."
USP20 affects HERC2
8 | 15 26
8 | 15 25

sparser
"Moreover, we show that p38 acts as a modulator of this pathway, since p38 activation disrupts HERC2-USP20 interaction, leading to increased USP20 and LC3-II protein levels."

reach
"In unstressed cells, HERC2 binds USP20 to maintain a low basal level of USP20 in cells."

reach
"During cell recovery from genotoxic stress, USP20 binds to HERC2 again and gets degraded."

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sparser
"Protein interaction of HERC2 with USP20 is regulated by p38 activation."

reach
"Chk1 activation is indirectly regulated via modulation of Claspin stability by ubiquitination and deubiquitination events, involving the HERC2 and USP20 complex [49,50]."

sparser
"To delve deeper into the molecular mechanisms underpinning HERC2-dependent autophagy regulation, we sought to determine whether HERC2 and USP20 proteins interact."

No evidence text available

sparser
"The results confirmed the interaction between HERC2 and USP20 proteins."

sparser
"Then, we hypothesized that p38 activity might modulate the interaction between HERC2 and USP20."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
USP20 affects CLSPN
2 7 1 | 24 5
USP20 binds CLSPN.
7 | 4 5
7 | 4 4

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sparser
"Further supporting a protective role for USP20/Claspin in cancer, it has been shown that Claspin expression in gastric cancer samples correlates with USP20 expression and that low Claspin and USP20 le[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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sparser
"We confirmed the USP20-Claspin interaction by Co-IP."

reach
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex and both USP20 and CLASPIN were in the HERC2 immunocomplex, suggesting that HERC2, USP20 and CLASPIN form a complex."

sparser
"To determine whether the interaction between USP20 and Claspin is direct, we generated and purified recombinant USP20 and Claspin."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
USP20 deubiquitinates CLSPN.
1 1 | 11
USP20 deubiquitinates CLSPN. 10 / 12
1 1 | 10

reach
"USP20 deubiquitinates and stabilizes Claspin."

reach
"CLASPIN was also deubiquitinated by bacterially produced GST-USP20, but not by the catalytically inactive mutant GST USP20 (C154S), in the in vitro deubiquitination assays."

reach
"Next, we tested whether USP20 deubiquitinates Claspin."

reach
"These results suggest that USP20 deubiquitinates Claspin in cells."

reach
"To determine whether USP20 directly deubiquitinates Claspin, we performed an in vitro deubiquitination assay."

reach
"These results revealed that USP20 mediated Claspin downregulation did not occur at the gene transcription level, just as the prior study reported that USP20 deubiquitinate and stabilize Claspin."

reach
"USP20 in turn deubiquitinates and stabilizes Claspin and enhances the activation of ATR-Chk1 signaling."

"USP20 deubiquitinates and stabilizes Claspin."

reach
"Taken together, these results suggest that USP20 deubiquitinates Claspin both in vitro and in vivo."

reach
"Unbound USP20 is enzymatically active and mediates Claspin deubiquitination and stabilization, this way promoting Chk1 activation [49,50]."
USP20 deubiquitinates ubiquitinated CLSPN. 1 / 1
| 1

reach
"USP20 in turn deubiquitinates K48-linked-polyubiquitinated CLASPIN, stabilizing CLASPIN and ultimately promoting CHK1 phosphorylation and CHK1 directed checkpoint activation."
USP20 activates CLSPN.
| 3
USP20 activates CLSPN. 3 / 9
| 3

reach
"USP20 promotes CLASPIN stability through deubiquitination."

reach
"These results revealed that USP20 mediated Claspin downregulation did not occur at the gene transcription level, just as the prior study reported that USP20 deubiquitinate and stabilize Claspin."

reach
"Given that HERC2 is a component of the replication complex, our data shown in this report suggest that HERC2 and USP20 coordinately modulate CLASPIN stability during S-phase and in response to replication stress."
USP20 increases the amount of CLSPN.
1 | 2
USP20 increases the amount of CLSPN. 2 / 3
1 | 1

reach
"Furthermore, reconstitution of WT USP20 but not catalytically inactive mutant of USP20 (USP20CA) in USP20 depleted cells restored Claspin protein levels."

"USP20 deubiquitinates and stabilizes Claspin."
Mutated USP20 increases the amount of CLSPN. 1 / 1
| 1

reach
"Furthermore, reconstitution of WT USP20 but not catalytically inactive mutant of USP20 (USP20CA) in USP20 depleted cells restored Claspin protein levels."
USP20 ubiquitinates CLSPN.
| 2
USP20 ubiquitinates CLSPN. 2 / 2
| 2

reach
"As shown in Figure XREF_FIG, overexpressing USP20 resulted in a significant decrease in polyubiquitination of Claspin, while overexpressing USP20CA mutant had no such effect."

reach
"Conversely, knocking-down USP20 increased polyubiquitination of Claspin."
USP20 inhibits CLSPN.
| 2
USP20 inhibits CLSPN. 2 / 2
| 2

reach
"This finding suggested that USP20 could inhibit cell proliferation of gastric cancer cells by regulating Claspin."

reach
"Furthermore, downregulation of USP20 blocked UV induced Claspin upregulation."
USP20 affects ULK1
4 1 | 12 16
USP20 binds ULK1.
4 | 5 16
4 | 5 16

sparser
"Notably, USP20 upregulation correlates with enhanced stability of the autophagy initiating kinase ULK1, highlighting the role of HERC2 as an autophagy regulator factor through the USP20-ULK1 axis."

sparser
"Our investigation unveils a novel role for HERC2 as an autophagy regulator factor through the USP20-ULK1 axis, providing new insights into the pathomolecular basis of HERC2-related disorder and Angelman syndrome."

sparser
"HERC2 regulates protein levels of the USP20-ULK1 axis."

sparser
"However, to date, no direct connection between HERC2, the USP20-ULK1 axis, and the regulation of autophagy has been reported."

sparser
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [ xref ]."

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reach
"At basal state, USP20 binds to and stabilizes ULK1 by removing the ubiquitin moiety, thereby interfering with the lysosomal degradation of ULK1."
USP20 deubiquitinates ULK1.
1 | 4
USP20 deubiquitinates ULK1. 5 / 5
1 | 4

"The deubiquitination of <span class="match term1">ULK1</span> by <span class="match term0">USP20</span> allows for its stabilization and promotion of autophagy induction"

reach
"USP20 has previously been shown to regulate autophagy by deubiquitinating and stabilizing ULK1, thus promoting autophagy [36]."

reach
"These observations are consistent with previously published data, which have established that USP20 deubiquitylates ULK1, safeguarding it from lysosomal degradation and enabling this kinase to effectively initiate the autophagic process [22]."

reach
"USP20 mediates ULK1 deubiquitination in the basal state, thus interfering with lysosome-dependent degradation of ULK1 and playing a key role in autophagy initiation [76]."

reach
"USP20 interacts with and deubiquitinates ULK1, thus protecting it from degradation."
USP20 activates ULK1.
| 2
USP20 activates ULK1. 2 / 2
| 2

reach
"It was also reported that USP20 promotes autophagy initiation by stabilizing ULK1 [21]."

reach
"USP20 induces autophagy by stabilizing ULK1 [41]."
USP20 ubiquitinates ULK1.
| 1
USP20 leads to the ubiquitination of ULK1. 1 / 1
| 1

reach
"The degradable ULK1 ubiquitination is reversed by USP20."
USP20 affects TRAF6
4 1 | 21 6
USP20 deubiquitinates TRAF6.
1 | 13
USP20 deubiquitinates TRAF6. 10 / 14
1 | 13

reach
"β-arrestin2 and USP20 inhibit the ubiquitination of TRAF6 through the shared mechanism."

reach
"54 Ubiquitinated beta-arrestin2 promotes NF-kappaB signaling in SMCs, whereas deubiquitinated beta-arrestin2 attenuates NF-kappaB signaling by scaffolding the deubiquitinase USP20, that in turn deubiquitinates TRAF6, which blocks the NF-kappaB signaling cascade."

"With purified proteins and in intact cells, our protein interaction studies showed that <span class="match term1">TRAF6</span>/<span class="match term0">USP20</span> association and subsequent <span class="match term0">USP20</span>-mediated <span class="match term1">TRAF6</span> deubiquitination were beta-arrestin2-dependent."

reach
"USP20 deubiquitinates TRAF6 and β-arrestin2, and the overexpression of β-arrestin2 is more effective in linking USP20 to TRAF6."

reach
"Here, we report that ubiquitin specific peptidase USP20 deubiquitinates TRAF6 and Tax and suppresses interleukin 1beta (IL-1beta) - and Tax induced NF-kappaB activation."

reach
"For example, Junichiro et al. demonstrated that USP21 constitutively deubiquitinates RIP1 in vitro and in vivo [XREF_BIBR]; and a previous study has reported that USP20 deubiquitinates TRAF6 and Tax in vivo [XREF_BIBR]."

reach
"For example, USP11 negatively regulates TNFalpha induced NF-kappaB activation associated with IkappaBalpha and attenuates IkappaBalpha degradation [XREF_BIBR]; USP20 deubiquitinates TRAF6 and suppresses interleukin 1beta (IL-1beta) - and Tax induced NF-kappaB activation [XREF_BIBR]; Katrin et al. showed that USP15 regulates IkappaBalpha and NF-kappaB by deubiquitinylation IkappaBalpha [XREF_BIBR]; and USP31 inhibits TNFalpha, CD40, TRAF2, TRAF6 and IKKbeta mediated NF-kappaB activation [XREF_BIBR]."

reach
"Together, these results suggest that IRAK1 inhibits USP20-mediated deubiquitination of TRAF6 by phosphorylating USP20 on Ser334 and reducing USP20:TRAF6 association."

reach
"Previous study indicated that USP20 can deubiquitinate TRAF6 and eliminate NF-κB-dependent gene expression, thereby suppressing inflammatory response during atherosclerosis development [13] ."

reach
"For example, USP20 deubiquitinates not only TRAF6 and β-arrestin2 to reduce TLR4-induced NFκB activation (3) but also RIPK1 to prevent TNFR-induced NFκB activation (4)."
USP20 binds TRAF6.
4 | 5
4 | 5

sparser
"The serine/threonine kinase IRAK1 is a key mediator of TRAF6 activation downstream of TLR4 and the IL-1R. Therefore, IRAK1 could not only promote TRAF6 activation but also prevent TRAF6 inactivation (deubiquitination)—if, indeed, IRAK1 phosphorylates USP20 on Ser334 (and thereby reduces the association of USP20 with TRAF6)."

No evidence text available

sparser
"Thus, it seems that preventing USP20 phosphorylation on Ser334 augments the association of USP20 with its intracellular substrate TRAF6, even though it has no effect on the association of USP20 with the idealized substrate Ub-VME ( xref , A and B )."

sparser
"Thus, IRAK1 not only transduces IL-1R signaling ( xref ) but also prevents its termination: phosphorylation of USP20 on Ser334 reduces USP20’s binding to TRAF6 and thereby diminishes (a) USP20-mediated deubiquitination/deactivation of TRAF6 and (b) consequent attenuation of downstream NFκB activation."

sparser
"Our findings reveal novel mechanisms regulating IL-1β-induced proinflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation, and neointimal hyperplasia."

No evidence text available

No evidence text available

sparser
"Together, these results suggest that IRAK1 inhibits USP20-mediated deubiquitination of TRAF6 by phosphorylating USP20 on Ser334 and reducing USP20:TRAF6 association."

No evidence text available
USP20 ubiquitinates TRAF6.
| 4 1
USP20 ubiquitinates TRAF6. 5 / 5
| 4 1

reach
"USP20 suppresses IL-1β- and Tax-induced NF-κB activation, by deubiquitinating TRAF6 and Tax, thus, inhibiting HTLV-1-transformed cell growth [132] ."

reach
"K63-linked poly-ubiquitination is significant for the activation of TRAF6, deubiquitinase A20, CYLD and USP20 have been reported to inhibit signal pathway transduction by removing the ubiquitination o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"(3) As a scaffold protein, β-arrestin2 forms a ternary complex with TRAF6 and ubiquitin-specific protease 20 (USP20), promoting the ubiquitination of TRAF6 by USP20 [ 33 ]."

reach
"The knockout of β-arrestin2 or USP20 enhances the ubiquitination of TRAF6 without cumulative effect [ 21 ]."

sparser
"The C-terminal of β-arrestin2 binds to TRAF6 after the activation of TLR4, preventing oligomerization and self-ubiquitination of TRAF6 to negatively regulate the activation of NFκB [ 33 , 56 ]. (2) Th[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 activates TRAF6.
| 3
USP20 activates TRAF6. 3 / 3
| 3

reach
"For example, USP17 and USP20 were found to target RIG-I and TRAF6 respectively, thereby functioning as novel regulators of antiviral innate immune responses [XREF_BIBR, XREF_BIBR]."

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"USP20, by promoting the stability of TRAF6 and Tax, contributes to the activation of NF-κB, including its activation induced by IL-1β."
USP20 inhibits TRAF6.
| 1
USP20 inhibits TRAF6. 1 / 1
| 1

reach
"Thus, IRAK1 not only transduces IL-1R signaling (1) but also prevents its termination: phosphorylation of USP20 on Ser334 reduces USP20’s binding to TRAF6 and thereby diminishes (a) USP20-mediated deubiquitination/deactivation of TRAF6 and (b) consequent attenuation of downstream NFκB activation.USP20 is a member of the USP subfamily of DUBs, which are endowed with protein interaction domains that also participate in the activation process of these enzymes (31)."
VHL affects USP20
1 4 1 | 14 5
VHL binds USP20.
4 | 8 3
4 | 8 2

reach
"Interestingly, several naturally occurring mutations in the VDU-binding domain of pVHL were found to disrupt the interaction between VDU1/VDU2 and pVHL, thus suggesting a functional relevance in pVHL-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Meanwhile, the structural basis for the interactions between USP20 DUSPs and its functional relevant proteins such as D2 and pVHL have not been elucidated yet."

sparser
"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."

No evidence text available

reach
"This mutation did not affect the interaction between hVDU2 and pVHL as predicted.VDU1 and VDU2 exhibit a very high homology at their N-terminal and C-terminal region as shown in Fig. 1, and both prote[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The interaction between hVDU2 and pVHL was also tested by in vivo co-immunoprecipitation."

No evidence text available

reach
"PVHL was precipitated by an anti-GFP antibody due to the interaction between hVDU2 and pVHL."

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
VHL ubiquitinates USP20.
1 | 6 2
VHL ubiquitinates USP20. 9 / 9
1 | 6 2

reach
"Moreover, VHL can ubiquitinate USP20 in return and target it for degradation [XREF_BIBR]."

reach
"Because pVHL can ubiquitylate both HIF-1α and VDU2, cellular HIF-1α levels might be determined by the balance between the pVHL-mediated ubiquitylation and VDU2-mediated de-ubiquitylation."

reach
"VDU2 itself can be ubiquitylated and degraded by the pVHL E3 ligase complex [32] ."

"Although <span class="match term0">pVHL</span> functions as a master control for HIF-1alpha stabilization, as <span class="match term0">pVHL</span>-E3 ligase mediates the ubiquitination of both HIF-1alpha and <span class="match term1">VDU2</span>, the balance between the <span class="match term0">pVHL</span>-mediated ubiquitination and <span class="match term1">VDU2</span>-mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization"

sparser
"Moreover, VHL can ubiquitinate USP20 in return and target it for degradation [ xref ]."

reach
"These results suggest that like hVDU1, the ubiquitination of hVDU2 is dependent on the presence of pVHL.To test whether hVDU2 ubiquitination mediated by pVHL could result in rapid turnover of hVDU2 pr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"To test whether hVDU2 can be ubiquitinated by pVHL E3 ubiquitin ligase complex, we performed ubiquitination assay in vitro and in vivo."

sparser
"Because pVHL can ubiquitylate both HIF-1α and VDU2, cellular HIF-1α levels might be determined by the balance between the pVHL-mediated ubiquitylation and VDU2-mediated de-ubiquitylation."

reach
"Although pVHL functions as a master control for HIF-1alpha stabilization, as pVHL-E3 ligase mediates the ubiquitination of both HIF-1alpha and VDU2, the balance between the pVHL mediated ubiquitination and VDU2 mediated deubiquitination of HIF-1alpha provides another level of control for HIF-1alpha stabilization."
VHL decreases the amount of USP20.
1 |
VHL bound to RBX1, CUL2, ELOC, and ELOB decreases the amount of USP20. 1 / 1
1 |

"VDU1 and VDU2 could be important substrates of pVHL E3 ligase complex. Finally, we demonstrate that VDU2 can also be ubiquitinated and degraded in a pVHL-dependent manner.pVHL mediates the degradation of hVDU2 by proteasome. we have demonstrated that both VDU1 and VDU2 also bind to the β-domain of pVHL and several naturally occurring mutations in the β-domain disrupt the interaction between VDU1/VDU2 and pVHL. If pVHL is mutated either in the α-domain or β-domain, VDU1 and VDU2 would not be ubiquitinated and degraded properly. This could lead to accumulation of these two proteins in the cells. Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL-related tumorigenesis."
ULK1 affects USP20
4 | 5 16
4 | 5 16

sparser
"Notably, USP20 upregulation correlates with enhanced stability of the autophagy initiating kinase ULK1, highlighting the role of HERC2 as an autophagy regulator factor through the USP20-ULK1 axis."

sparser
"Our investigation unveils a novel role for HERC2 as an autophagy regulator factor through the USP20-ULK1 axis, providing new insights into the pathomolecular basis of HERC2-related disorder and Angelman syndrome."

sparser
"HERC2 regulates protein levels of the USP20-ULK1 axis."

sparser
"However, to date, no direct connection between HERC2, the USP20-ULK1 axis, and the regulation of autophagy has been reported."

sparser
"However, the interaction between ULK1 and USP20 is weakened upon prolonged induction of autophagy (4 to 8 h), leading to a reduction of the level of ULK1 protein while its ubiquitinated form accumulates [ xref ]."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

reach
"At basal state, USP20 binds to and stabilizes ULK1 by removing the ubiquitin moiety, thereby interfering with the lysosomal degradation of ULK1."
USP20 affects YAP1
| 12 10
USP20 binds YAP1.
| 2 10
| 2 10

sparser
"USP20 directly interacts with YAP1 in a DUB-dependent protease-related regulatory mechanism."

sparser
"To investigate the regulatory mechanism of USP20-mediated upregulation of YAP1 expression, reciprocal co-immunoprecipitation was performed in T24 cells to identify a potential physical interaction between USP20 and YAP1 ( xref A)."

sparser
"The results of a GST pull-down assay confirmed that USP20 and YAP1 directly bind to each other ( xref B)."

sparser
"We monitored the direct interaction between USP20 and YAP1 using immunofluorescence ( xref D) and Duolink proximity ligation assays ( xref F)."

sparser
"These results indicated that USP20 directly interacts with YAP1 and increases its stability by preventing ubiquitin-proteasome pathway activity."

reach
"To investigate the regulatory mechanism of USP20-mediated upregulation of YAP1 expression, reciprocal co-immunoprecipitation was performed in T24 cells to identify a potential physical interaction between USP20 and YAP1 (Fig. 4A)."

reach
"Thus, we performed further experiments to determine the details of the interaction between USP20 and YAP1."

sparser
"Co-IP and GST pull-down assays indicated that USP20 directly interacted with YAP1 ( xref )."

sparser
"Thus, we performed further experiments to determine the details of the interaction between USP20 and YAP1."

sparser
"For interaction analysis, deleted constructs were cloned and analyzed, which revealed that the WW domain of YAP1 is essential for its interaction with USP20 ( xref A) and that the UCH domain is required for the direct interaction of USP20 with YAP1 ( xref B)."
USP20 increases the amount of YAP1.
| 5
USP20 increases the amount of YAP1. 5 / 5
| 5

reach
"Further analysis revealed that USP20 directly regulated the expression of YAP1 and its downstream target genes connective tissue growth factor and cysteine-rich angiogenic inducer 61."

reach
"Overexpression of USP20 upregulated YAP1 expression."

reach
"USP20 overexpression in BC cells significantly increased YAP1 expression, whereas treatment with si-USP20 decreased YAP1 expression (Fig.S1A)."

reach
"USP20 knockdown in BC cells significantly decreased YAP1 expression, whereas YAP1 overexpression reversed this effect (Fig.S3)."

reach
"USP20 knockdown significantly decreased the protein levels of YAP1, whereas MG132 treatment diminished this effect (Fig. 4C)."
USP20 activates YAP1.
| 3
USP20 activates YAP1. 3 / 3
| 3

reach
"Based on the above results, we investigated whether USP20-mediated YAP1 stabilization was mediated by the direct deubiquitination of USP20."

reach
"Our results indicated that USP20 deubiquitinated and increased the protein stability of YAP1 by removing the K48-linked polyubiquitin chains from YAP1."

reach
"Through direct interaction with YAP1, USP20 modulates the activity and stability of YAP1 to exert its biological functions via the transition between activated or deactivated Hippo.Combination therapy has only slightly improved the overall survival of patients with BC."
USP20 decreases the amount of YAP1.
| 2
USP20 decreases the amount of YAP1. 2 / 2
| 2

reach
"USP20 overexpression in BC cells significantly increased YAP1 expression, whereas treatment with si-USP20 decreased YAP1 expression (Fig.S1A)."

reach
"Furthermore, an in vitro ubiquitination experiment demonstrated that the purified USP20 protein decreased the ubiquitination level of YAP1, but the USP20 form did not (Fig. 6E)."
USP20 affects HIF1A
3 1 | 10 5
USP20 binds HIF1A.
3 | 3 5
3 | 3 5

reach
"The interaction between HIF-1alpha and VDU2 was shown using an immunoprecipitation assay."

sparser
"However, by using in vitro pull down assays with GST-HIF-1α (amino acid 530–826) and co-immunoprecipitation experiments in COS-7 cells it was shown that only VDU2 but not VDU1 could interact with HIF-1α (Li et al., xref )."

sparser
"In contrast to VDU2 which directly interacts with HIF-1α, USP28 forms a ternary complex with HIF-1α via its association with HIF-1α bound Fbw7 (Flügel et al., xref )."

sparser
"VDU2 can interact with HIF-1α, a tumor suppressor, to deubiquitinate and stabilize HIF-1α ( xref )."

reach
"Molecular and cellular experiments showed that VDU2 interacted with HIF-1alpha in order to induce the expression of HIF-1 downstream genes, such as VEGF, through the deubiquitination and stabilization of HIF-1alpha."

sparser
"VDU2 binds and de-ubiquitylates HIF-1α in a pVHL-dependent manner, hence salvaging it from proteasomal degradation."

sparser
"According to those findings, USP20 binds to and stabilizes HIF-1α."

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."

No evidence text available

No evidence text available
USP20 deubiquitinates HIF1A.
1 | 4
USP20 deubiquitinates HIF1A. 5 / 5
1 | 4

reach
"VDU2 can specifically deubiquitinate and stabilize HIF-1alpha and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF)."

reach
"One of them, USP-20, deubiquitinates HIF-1alpha and therefore tends to stabilize it [46] by preventing its proteasomal degradation."

reach
"In addition, VDU2, but not VDU1, is able to interact with and deubiquitinate HIF1alpha, and rescue it from proteasome mediated degradation."

"<span class="match term0">USP20</span> stabilizes <span class="match term1">HIF-1ALPHA</span> by abolishing von Hippel-Lindau protein (pVHL)-E3 ligase complex-mediated <span class="match term1">HIF-1ALPHA</span> degradation"

reach
"In addition, it was shown that VDU2 but not VDU1 can deubiquitylate HIF-1alpha and increase it 's half-life."
USP20 activates HIF1A.
| 2
USP20 activates HIF1A. 2 / 4
| 2

reach
"Therefore, targeting USP20 can induce degradation of HIF-1alpha and be a potential drug target for human cancer."

reach
"Up-regulation of Usp20, a deubiquitinase that acts on Hif1a and other proteins 24, should reduce degradation of Hif1a."
USP20 increases the amount of HIF1A.
| 1
USP20 increases the amount of HIF1A. 1 / 1
| 1

reach
"The previous study has shown that Usp20 increases the expression of HIF1A XREF_BIBR, XREF_BIBR, which is reported as a metabolic switch for an early stage of iPSC 20."
USP33 affects USP20
2 | 6 12
2 | 6 4

No evidence text available

No evidence text available

sparser
"USP33 and USP20 likely interact with the ER membrane bilayer via their amphipathic helices since they do not have transmembrane domains [ xref ]."

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."

reach
"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays (XREF_SUPPLEMENTARY) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 (XREF_SUPPLEMENTARY)."

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Two DUBs, USP33 (or VDU-1) and USP20 (or VDU-2), interact with and regulate D2 [ xref ]."

reach
"Interestingly, several naturally occurring mutations in the VDU-binding domain of pVHL were found to disrupt the interaction between VDU1/VDU2 and pVHL, thus suggesting a functional relevance in pVHL-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
ARs binds USP33 and USP20. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."

reach
"Low expression of USP20 significantly promoted cell proliferation and accelerated the transition from G1 to S phase of the cell cycle [60]."

reach
"Depletion of several DUBs caused either resistance or hypersensitivity to HU treatment; among them, USP20 knockdown showed most significant hypersensitivity to HU treatment, while knockdown of USP20 slightly decreased cell proliferation in untreated cells."

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."

reach
"However, these data are contradicted by recent findings showing that USP20 promotes proliferation, invasion, migration, and chemoresistance of multiple cancer cells via deubiquitylation of β-catenin [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"67 A recent study reported that USP20 stabilizes β‐catenin and activates Wnt/β‐catenin signaling pathway, thereby promoting the migration, invasion, and proliferation of cancer cells."

reach
"These finding revealed that USP20 could modulate GC cell proliferation."

reach
"And they also observed that USP20 overexpression markedly increased cell proliferation, migration and invasion in these cancer cell lines."

reach
"Basic experiments verified that USP20 overexpression promoted both the proliferation and migration of breast cancer cells."

reach
"Altogether, these findings and Figure 4 suggest that phosphorylation of USP20 on Ser334 augments neointimal hyperplasia primarily by increasing inflammatory signaling and associated proliferation of SMCs.To model neointimal hyperplasia in vitro (16, 17), we assessed proliferation and migration of SMCs derived from WT and USP20-S334A mice."

reach
"The EdU assay indicated that USP20 silencing significantly decreased the number of EdU-positive BC cells, whereas USP20 overexpression increased the proliferation rate of BC cells (Fig. 3C)."

reach
"This finding suggested that USP20 could inhibit cell proliferation of gastric cancer cells by regulating Claspin."

reach
"In line with the clinical outcomes, we found that the overexpression of USP20 could, on one hand, suppress cell proliferation and delay G1-S cell cycle transition, and on the other hand, enhance autophagy in GC cells."

reach
"Silencing of USP20 increases proliferation in GC cells."

reach
"USP20 stabilizes claspin and inhibits proliferation, G1–S cell cycle transition, and autophagy in gastric cancer cells [23] ."

reach
"Experimentally, small interfering RNA mediated knockdown of USP20 expression significantly promoted cell proliferation, accelerated G1-S phase transition and attenuated the autophagy activity."

reach
"Since overexpression of USP20 inhibits the proliferation of ATL cells, it raises the possibility that induction of USP20 could be used to inhibit HTLV induced oncogenesis."

reach
"Past reports have indicated that USP20 overexpression inhibited cell proliferation and delayed the cell cycle transition from the G1 to S phase by stabilizing Claspin [43,170]."

reach
"In HTLV-1-transformed cells, the transcription of USP20 is reduced compared with HTLV-1-negative T cells, and ectopic USP20 expression was found to inhibit the proliferation of an HTLV-1-transformed cell line, MT4."
| PMC

reach
"Through deubiquitination of TRAF6 and Tax, USP20 suppresses activation of NF-κB signaling and inhibits proliferation of leukemia cells (53)."
USP20 affects USP33
2 | 6 12
2 | 6 4

No evidence text available

No evidence text available

sparser
"USP33 and USP20 likely interact with the ER membrane bilayer via their amphipathic helices since they do not have transmembrane domains [ xref ]."

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."

reach
"In the current studies and together with our previous report [30], we have demonstrated that both VDU1 and VDU2 also bind to the beta-domain of pVHL and several naturally occurring mutations in the be[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays (XREF_SUPPLEMENTARY) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 (XREF_SUPPLEMENTARY)."

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Two DUBs, USP33 (or VDU-1) and USP20 (or VDU-2), interact with and regulate D2 [ xref ]."

reach
"Interestingly, several naturally occurring mutations in the VDU-binding domain of pVHL were found to disrupt the interaction between VDU1/VDU2 and pVHL, thus suggesting a functional relevance in pVHL-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
ARs binds USP33 and USP20. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP20 affects STING1
3 1 | 5 11
USP20 binds STING1.
3 | 10
3 | 6

No evidence text available

sparser
"The present study demonstrated that USP20 interacted with STING and facilitated its degradation, consequently alleviating the myocardial inflammation and improving ventricular remodeling and heart failure induced by diabetes."

sparser
"Domain‐focused Co‐IP analyzes revealed that USP20 binds exclusively to STING via its catalytic USP domain, a conclusion supported by the complete loss of interaction in the catalytic USP domain mutant (Figure  xref )."

sparser
"In mechanism, USP20 directly binds to STING and promotes its degradation through the autophagy pathway by deubiquitinating p62 via its active site C154, thereby alleviating the myocardial inflammation and improving ventricular remodeling and heart failure induced by diabetes."

No evidence text available

sparser
"USP20 directly binds to STING and promotes the degradation of STING through the autophagy pathway by deubiquitinating p62 via its active site C154, thereby alleviating myocardial inflammation and improving ventricular remodeling and heart failure induced by diabetes."

No evidence text available

sparser
"Notably, STING emerged as the only overlapping candidate substrate across all three groups (Figure  xref ), prompting us to investigate whether an interaction exists between USP20 and STING."

sparser
"We then examined the specific domains through which USP20 interacts with STING."
| 4

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."
USP20 inhibits STING1.
| 2
USP20 inhibits STING1. 2 / 2
| 2

reach
"On the other hand, OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation, thus maintain the stability of STING and promote activation of STING pathway (87–89)."

reach
"USP18 deficiency or knockdown of USP20 resulted in enhanced K48 linked ubiquitination and accelerated degradation of STING, and impaired activation of IRF3 and NF-kappaB as well as induction of downstream genes after infection with DNA virus HSV-1 or transfection of various DNA ligands."
USP20 deubiquitinates STING1.
1 | 1
USP20 leads to the deubiquitination of STING1. 2 / 2
1 | 1

reach
"The USP family of DUBs is very important, with USP13, USP18, USP20, USP21, USP44, and USP49 shown to mediate the deubiquitination of STING (Chen et al., 2021)."

"USP18 recruits <span class="match term0">USP20</span> to promote innate antiviral response through deubiquitinating <span class="match term1">STING</span>/MITA"
USP20 activates STING1.
| 2
USP20 activates STING1. 2 / 2
| 2

reach
"On the other hand, OTUD5 and USP20 catalyze the K48-linked deubiquitination of STING and inhibit STING degradation, thus maintain the stability of STING and promote activation of STING pathway (87–89)."

reach
"Besides, USP18 can interact with USP20, and USP20 can enhance STING stability [48] ."
USP20 phosphorylates STING1.
| 1
USP20 phosphorylates STING1. 1 / 1
| 1

sparser
"Given that higher levels of the STING protein are expected to correlate with increased phosphorylated STING, it might be hypothesized that USP20 naturally enhances STING phosphorylation in cardiomyocytes."
USP20 affects SQSTM1
2 1 | 3 14
USP20 binds SQSTM1.
2 | 1 14
2 | 1 14

sparser
"Based on this speculation, our current experiments provide evidence that the p62-USP20 axis is required for PKCζ-mediated NF-κB activation upon TNFα treatment."

sparser
"These findings strongly suggest that the USP20-p62 axis plays an essential role in NF-κB-mediated cell survival induced by the TNFα-atypical PKCζ signaling pathway."

sparser
"Herein, we find that USP20 binds to p62 at 5 min upon TNFα treatment and thus stabilizes it through removing the lysine 48 (K48)-linked polyubiquitin chains."

sparser
"USP20 specifically binds to p62 and acts as a positive regulator for NF-κB activation by TNFα through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival."

sparser
"We speculate that the USP20-p62 axis works on the upstream of RIPK1 in non-classical PKCζ-mediated NF-κB activation upon TNFα treatment and USPS20 depletion decreases p62 scaffolding activity, resulting in the release of RIPK1."

sparser
"This report is the first to reveal the role of the USP20-p62 axis in NF-κB-mediated cell survival."

No evidence text available

sparser
"This is the first report that reveals the molecular mechanism of the USP20-p62 axis regarding NF-κB-mediated cell survival."

sparser
"Co‐IP assays were conducted, confirming that USP20 interacts with p62 but not with LC3 (Figure  xref )."

No evidence text available
USP20 activates SQSTM1.
| 2
USP20 activates SQSTM1. 2 / 2
| 2

reach
"In their study, they found that USP20 increased the stability of p62 by deubiquitinating lysine 48 (K48)-linked polyubiquitination, promoting cell survival."

reach
"This study was conducted in a cervical cancer model and uncovered that USP20-mediated p62 stabilization and subsequent upregulation of p65/p50 activity contribute to cancer cell survival (24)."
USP20 deubiquitinates SQSTM1.
1 |
USP20 deubiquitinates SQSTM1. 1 / 1
1 |

"<span class="match term0">USP20</span> specifically binds to <span class="match term1">p62</span> and acts as a positive regulator for NF-kappaB activation by TNFalpha through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival"
USP20 affects HSPA2
3 | 5 12
USP20 binds HSPA2.
3 | 2 12
3 | 2 12

No evidence text available

sparser
"These results were consistent with our findings that USP20 interacted with HSPA2 and stabilized HSPA2 via deubiquitination."

sparser
"Our study identified a new pathway involved in SG where the downregulation of USP20-HSPA2 axis improved lipid metabolism."

sparser
"The downregulation of the USP20-HSPA2 axis in diet-induced obese mice following SG improved lipid dysmetabolism, indicating that USP20-HSPA2 axis was a noninvasive therapeutic target to be investigated in the future."

sparser
"Therefore, fatty acid biosynthesis and cholesterol transport are the possible pathways by which the USP20-HSPA2 axis achieves biological functions."

reach
"These results indicated an interaction between USP20 and HSPA2, and owing to the deubiquitinating enzyme function of USP20, we tested whether USP20 induced the deubiquitination of HSPA2."

sparser
"Some limitations of this study include the necessity of in vivo experiments to verify the effect of the USP20-HSPA2 axis on lipid metabolism."

sparser
"The downstream pathway of the USP20-HSPA2 axis needs further studies for validation."

sparser
"In conclusion, our study provides evidence that the USP20-HSPA2 axis is downregulated in the SG group."

sparser
"To gain insight into the USP20-HSPA2 axis mechanism, we first examined whether there was an interaction between USP20 and HSPA2."
USP20 deubiquitinates HSPA2.
| 2
USP20 leads to the deubiquitination of HSPA2. 2 / 2
| 2

reach
"As shown in Figure 5E , Myc-HSPA2 was ubiquitinated in the presence of HA-ubiquitin, and the co-expression of Flag-USP20 reduced the ubiquitination of Myc-HSPA2."

reach
"These results indicated an interaction between USP20 and HSPA2, and owing to the deubiquitinating enzyme function of USP20, we tested whether USP20 induced the deubiquitination of HSPA2."
USP20 inhibits HSPA2.
| 1
USP20 inhibits HSPA2. 1 / 1
| 1

reach
"The results indicated that overexpression and knockdown of USP20 caused respective upregulation and downregulation of HSPA2 at the protein level ( Figure 4A )."
SQSTM1 affects USP20
2 | 1 14
2 | 1 14

sparser
"Based on this speculation, our current experiments provide evidence that the p62-USP20 axis is required for PKCζ-mediated NF-κB activation upon TNFα treatment."

sparser
"These findings strongly suggest that the USP20-p62 axis plays an essential role in NF-κB-mediated cell survival induced by the TNFα-atypical PKCζ signaling pathway."

sparser
"Herein, we find that USP20 binds to p62 at 5 min upon TNFα treatment and thus stabilizes it through removing the lysine 48 (K48)-linked polyubiquitin chains."

sparser
"USP20 specifically binds to p62 and acts as a positive regulator for NF-κB activation by TNFα through deubiquitinating lysine 48 (K48)-linked polyubiquitination, eventually contributing to cell survival."

sparser
"We speculate that the USP20-p62 axis works on the upstream of RIPK1 in non-classical PKCζ-mediated NF-κB activation upon TNFα treatment and USPS20 depletion decreases p62 scaffolding activity, resulting in the release of RIPK1."

sparser
"This report is the first to reveal the role of the USP20-p62 axis in NF-κB-mediated cell survival."

No evidence text available

sparser
"This is the first report that reveals the molecular mechanism of the USP20-p62 axis regarding NF-κB-mediated cell survival."

sparser
"Co‐IP assays were conducted, confirming that USP20 interacts with p62 but not with LC3 (Figure  xref )."

No evidence text available
HSPA2 affects USP20
3 | 2 12
3 | 2 12

No evidence text available

sparser
"These results were consistent with our findings that USP20 interacted with HSPA2 and stabilized HSPA2 via deubiquitination."

sparser
"Our study identified a new pathway involved in SG where the downregulation of USP20-HSPA2 axis improved lipid metabolism."

sparser
"The downregulation of the USP20-HSPA2 axis in diet-induced obese mice following SG improved lipid dysmetabolism, indicating that USP20-HSPA2 axis was a noninvasive therapeutic target to be investigated in the future."

sparser
"Therefore, fatty acid biosynthesis and cholesterol transport are the possible pathways by which the USP20-HSPA2 axis achieves biological functions."

reach
"These results indicated an interaction between USP20 and HSPA2, and owing to the deubiquitinating enzyme function of USP20, we tested whether USP20 induced the deubiquitination of HSPA2."

sparser
"Some limitations of this study include the necessity of in vivo experiments to verify the effect of the USP20-HSPA2 axis on lipid metabolism."

sparser
"The downstream pathway of the USP20-HSPA2 axis needs further studies for validation."

sparser
"In conclusion, our study provides evidence that the USP20-HSPA2 axis is downregulated in the SG group."

sparser
"To gain insight into the USP20-HSPA2 axis mechanism, we first examined whether there was an interaction between USP20 and HSPA2."
USP20 affects VHL
4 | 9 3
USP20 binds VHL.
4 | 8 3
4 | 8 2

reach
"Interestingly, several naturally occurring mutations in the VDU-binding domain of pVHL were found to disrupt the interaction between VDU1/VDU2 and pVHL, thus suggesting a functional relevance in pVHL-[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Meanwhile, the structural basis for the interactions between USP20 DUSPs and its functional relevant proteins such as D2 and pVHL have not been elucidated yet."

sparser
"The proteasome has both ubiquitin ligases and DUBs that associate with it (Crosas et al., 2006) , and several DUB-ligase pairs interact directly, including BRCC36-BRCA1, BAP1-BRCA1, USP4-Ro52, USP7-MDM2, USP8-GRAIL, USP20-pVHL, USP33-pVHL and USP44-APC (Kee and Huibregtse, 2007; Marfany and Denuc, 2008; Ventii and Wilkinson, 2008) ."

No evidence text available

reach
"This mutation did not affect the interaction between hVDU2 and pVHL as predicted.VDU1 and VDU2 exhibit a very high homology at their N-terminal and C-terminal region as shown in Fig. 1, and both prote[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The interaction between hVDU2 and pVHL was also tested by in vivo co-immunoprecipitation."

No evidence text available

reach
"PVHL was precipitated by an anti-GFP antibody due to the interaction between hVDU2 and pVHL."

reach
"Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL related tumorigenesis.In conclusion, our previously published results and current data indicate that the interaction between [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Further investigations are warranted to probe the biochemical and biological functions of the interaction between pVHL and VDU1 and VDU2 and to dissect functional and physiological differences between[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
USP20 activates VHL.
| 1
USP20 activates VHL. 1 / 1
| 1

reach
"Considering that USP20 is a substrate of the pVHL protein, we speculate that pVHL protein inactivation induces decreased degradation of USP20, promoting the progression of VHL disease."
CLSPN affects USP20
7 | 4 5
7 | 4 4

No evidence text available

No evidence text available

No evidence text available

No evidence text available

sparser
"Further supporting a protective role for USP20/Claspin in cancer, it has been shown that Claspin expression in gastric cancer samples correlates with USP20 expression and that low Claspin and USP20 le[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

No evidence text available

No evidence text available

sparser
"We confirmed the USP20-Claspin interaction by Co-IP."

reach
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex and both USP20 and CLASPIN were in the HERC2 immunocomplex, suggesting that HERC2, USP20 and CLASPIN form a complex."

sparser
"To determine whether the interaction between USP20 and Claspin is direct, we generated and purified recombinant USP20 and Claspin."
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
IRAK1 affects USP20
| 7 8
IRAK1 phosphorylates USP20.
| 6 8
IRAK1 phosphorylates USP20 on S333. 10 / 10
| 5 5

sparser
"We hypothesized that in the context of IL-1β signaling, perhaps other serine/threonine kinases, including IRAK1 might also phosphorylate USP20 Ser334."

sparser
"Thus, IRAK1 can phosphorylate USP20 on Ser333 (of the human USP20 sequence) in preparations of purified proteins."

sparser
"Does IRAK1 phosphorylate mouse USP20 on Ser334 in intact SMCs?"

sparser
"Hence, we infer that IRAK1 knockdown is specific in SMCs, and SMC IRAK1 phosphorylates USP20 Ser334."

reach
"Therefore, IRAK1 could not only promote TRAF6 activation but also prevent TRAF6 inactivation (deubiquitination)—if, indeed, IRAK1 phosphorylates USP20 on Ser334 (and thereby reduces the association of USP20 with TRAF6)."

reach
"Thus, IRAK1 can phosphorylate USP20 on Ser333 (of the human USP20 sequence) in preparations of purified proteins.Does IRAK1 phosphorylate mouse USP20 on Ser334 in intact SMCs?"

reach
"Together, these results suggest that IRAK1 inhibits USP20-mediated deubiquitination of TRAF6 by phosphorylating USP20 on Ser334 and reducing USP20:TRAF6 association."

reach
"In preparations of purified proteins and in intact SMCs, we found that phosphorylation of USP20 on Ser334 is mediated by IRAK1."

sparser
"Our studies revealed that USP20 Ser334 is phosphorylated by IRAK1."

reach
"Our studies revealed that USP20 Ser334 is phosphorylated by IRAK1."
IRAK1 phosphorylates USP20. 4 / 4
| 1 3

sparser
"IRAK1 phosphorylates USP20 in purified preparations and in intact SMCs."

sparser
"To test this possibility, we used purified IRAK1 to phosphorylate human USP20 purified from COS-7 cells ( xref )."

reach
"IRAK1 phosphorylates USP20 in purified preparations and in intact SMCs."

sparser
"IL-1β signaling was also found to be promoted by IRAK1 induced phosphorylation of USP20 in vascular smooth muscle cells and vascular inflammation [ xref , xref ]."
IRAK1 dephosphorylates USP20.
| 1
IRAK1 dephosphorylates USP20 on S333. 1 / 1
| 1

reach
"Together, these results suggest that IRAK1 inhibits USP20-mediated deubiquitination of TRAF6 by phosphorylating USP20 on Ser334 and reducing USP20:TRAF6 association."
USP20 affects SLC7A11
| 11 3
USP20 deubiquitinates SLC7A11.
| 5
USP20 deubiquitinates SLC7A11. 5 / 5
| 5

reach
"Ectopic expression of USP20‐WT, but not USP20 , markedly reduced the ubiquitylation of SLC7A11."

reach
"In vivo deubiquitylation assays showed that USP20 reduced SLC7A11 polyubiquitination in a time‐ and dose‐dependent manner."

reach
"To Further analysis indicated that K30 and K37 are the key sites on SLC7A11 deubiquitinated by USP20."

reach
"Conversely, the deubiquitinating enzymes OTUB1 and ubiquitin specific peptidase 20 (USP20) mediate the deubiquitination of SLC7A11, thereby stabilizing SLC7A11 levels in cancer cells [6,122]."

reach
"Ubiquitination assay indicated that K30 and K37 were the key sites on SLC7A11 deubiquitinated by USP20 (Figure 5G)."
USP20 increases the amount of SLC7A11.
| 4
USP20 increases the amount of SLC7A11. 4 / 4
| 4

reach
"Conversely, the deubiquitinating enzymes OTUB1 and ubiquitin specific peptidase 20 (USP20) mediate the deubiquitination of SLC7A11, thereby stabilizing SLC7A11 levels in cancer cells [6,122]."

reach
"We observed that USP20 depletion significantly reduced the protein expression of SLC7A11, and such effect could be blocked by the addition of MG132 (proteasome inhibitor) or overexpression of wild‐type USP20, but not its catalytically inactive mutant (Figures 4B and C)."

reach
"As expected, USP20 depletion remarkably reduced the protein levels of SLC7A11 without influence on the mRNA level (Figure 4A)."

reach
"Since USP20 is a DUB belonging to the ubiquitin‐specific processing protease family and USP20 depletion decreased SLC7A11 protein levels, suggesting that USP20 may control the protein stability of SLC7A11 via the Ub–proteasome system."
USP20 binds SLC7A11.
| 1 2
| 1 2

reach
"Results of Co‐IP revealed the association between SLC7A11 and USP20."

sparser
"First, USP20 and SLC7A11 binds with each other."

sparser
"Recently, it was reported that USP20 directly interacts with xCT and cleaves K48‐linked polyubiquitinated xCT at K30 and K37. [ xref ] TRIM3, an E3 ubiquitin ligase, promoted xCT proteasome‐dependent degradation by catalyzing K11‐linked ubiquitination of xCT at K37. [ xref ] Notably, no reports have identified any E3 ligase‐mediated ubiquitination of the xCT protein at K4 or K12, leaving an open question regarding the identification of another antagonistic regulator responsible for ferroptosis sensitivity and ubiquitination modification of xCT at K4 or K12 in BLCA in future studies."
USP20 ubiquitinates SLC7A11.
| 1 1
USP20 leads to the ubiquitination of SLC7A11. 2 / 2
| 1 1

reach
"Conversely, ectopic expression of USP20‐WT significantly decreased ubiquitinated SLC7A11 in cells (Figure 5B)."

sparser
"Conversely, ectopic expression of USP20‐WT significantly decreased ubiquitinated SLC7A11 in cells (Figure  xref )."
USP20 affects MCL1
| 12 2
USP20 increases the amount of MCL1.
| 5
USP20 increases the amount of MCL1. 5 / 5
| 5

reach
"We found that USP20 but not its enzymatically inactive mutant USP20–C154S could up-regulate the protein level of MCL1 ( Fig. 2 a)."

reach
"Consistent with our above finding, when contrasted with full-length USP20, only the USP20 construct bearing the enzymatic domain, aa 145–689 but not aa 1–144 and 690–914, significantly increased the M[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Moreover, knockdown of USP20 could reduce the level of MCL1 and render cells more sensitive to the BH3 mimetic compound, ABT-263."

reach
"Moreover, USP20 expression could also up-regulate the endogenous MCL1 protein level without influencing the MCL1 RNA expression level ( Fig. 1 c and d)."

reach
"Furthermore, knockdown of USP20 decreased MCL1 protein levels ( Fig. 1 e)."
USP20 deubiquitinates MCL1.
| 3
USP20 deubiquitinates MCL1. 3 / 3
| 3

reach
"We identified that USP20 knockdown significantly increased the polyubiquitination of MCL1 compared to the control (Ctrl) cells ( Fig. 3 f)."

reach
"Since MCL1 plays a key role in the development of human cancers, it is highly probable that USP20 could promote the deubiquitination and stabilization of MCL1 in human cancers."

reach
"Our findings indicate that USP20 deubiquitinates MCL1 and regulates its downstream cellular functions."
USP20 binds MCL1.
| 1 2
| 1 2

reach
"As shown in Fig. 3 a and b, the coimmunoprecipitation assay revealed a significant endogenous binding between USP20 and MCL1."

sparser
"To further probe the mechanism by which USP20 regulates MCL1, we examined whether USP20 interacted with MCL1."

sparser
"We also found that USP20 could interact with MCL1 by removing its K48-linked ubiquitin chain."
USP20 inhibits MCL1.
| 2
USP20 inhibits MCL1. 2 / 2
| 2

reach
"As shown in Fig. 4 d, USP20 diminished the down-regulation of MCL1 induced by Sorafenib in ECA-109 cells."

reach
"Next, we investigated whether the overexpression of USP20 could reverse the down-regulation of MCL1 induced by Sorafenib."
USP20 activates MCL1.
| 1
USP20 activates MCL1. 1 / 1
| 1

reach
"As shown in Fig. 1 f, USP20 expression significantly increased the stability of the MCL1 protein.Next, we investigated how USP20 stabilizes MCL1."
YAP1 affects USP20
| 3 10
YAP1 binds USP20.
| 2 10
| 2 10

sparser
"USP20 directly interacts with YAP1 in a DUB-dependent protease-related regulatory mechanism."

sparser
"To investigate the regulatory mechanism of USP20-mediated upregulation of YAP1 expression, reciprocal co-immunoprecipitation was performed in T24 cells to identify a potential physical interaction between USP20 and YAP1 ( xref A)."

sparser
"The results of a GST pull-down assay confirmed that USP20 and YAP1 directly bind to each other ( xref B)."

sparser
"We monitored the direct interaction between USP20 and YAP1 using immunofluorescence ( xref D) and Duolink proximity ligation assays ( xref F)."

sparser
"These results indicated that USP20 directly interacts with YAP1 and increases its stability by preventing ubiquitin-proteasome pathway activity."

reach
"To investigate the regulatory mechanism of USP20-mediated upregulation of YAP1 expression, reciprocal co-immunoprecipitation was performed in T24 cells to identify a potential physical interaction between USP20 and YAP1 (Fig. 4A)."

reach
"Thus, we performed further experiments to determine the details of the interaction between USP20 and YAP1."

sparser
"Co-IP and GST pull-down assays indicated that USP20 directly interacted with YAP1 ( xref )."

sparser
"Thus, we performed further experiments to determine the details of the interaction between USP20 and YAP1."

sparser
"For interaction analysis, deleted constructs were cloned and analyzed, which revealed that the WW domain of YAP1 is essential for its interaction with USP20 ( xref A) and that the UCH domain is required for the direct interaction of USP20 with YAP1 ( xref B)."
YAP1 activates USP20.
| 1
YAP1 activates USP20. 1 / 1
| 1

reach
"Thus, the inhibition of Hippo-YAP1 pathway-related DUBs may directly enhance the ubiquitination and degradation of the YAP1 protein, which could be a promising strategy to disrupt the function of the Hippo-YAP1 pathway and inhibit the progression of BC.Our siRNA screening assay identified USP20 as a critical USP that regulates YAP1 stability and the malignant phenotype of BC."
USP20 affects Tmc1
| 13
| 13

sparser
"The angiogenic sprouting of MCECs is diminished by the expression of WT and phospho-resistant USP20-S334A, while it is exacerbated by USP20-DN and phospho-mimetic USP20-S334D."

sparser
"To test the role of USP20 and its phosphorylation status in EC sprouting, we performed the spheroid-based sprouting assay using MCECs transduced with recombinant adenoviruses, expressing USP20-WT, USP20-DN, USP20-S334A, and USP20-S334D ( xref – xref )."

sparser
"NFκB activity was augmented in MCECs with overexpression of USP20-DN but diminished with exogenous expression of USP20 WT ( xref )."

sparser
"We therefore evaluated the effect of USP20 (USP20-WT), inactive USP20 (USP20-dominant negative or USP20-DN), phospho-resistant USP20 (USP20-S334A), and phospho-mimetic USP20 (USP20-S334D) on NFκB activation triggered by IL-1β."

sparser
"Assessed by scratch-wound healing and spheroid assays, migration and angiogenesis of MCECs, respectively, were (a) increased by USP20-DN or USP20(S334D), and (b) decreased by USP20-WT or USP20(S334A)."

sparser
"USP20-WT and USP20-S334A transduced MCECs formed weak or no sprouts from spheroids compared with the GFP-treated MCECs ( xref ), whereas angiogenic sprouting was significantly increased in USP20-DN and USP20-S334D transduced MCECs compared with the GFP-treated MCECs ( xref )."

sparser
"Data from our in vitro spheroid assay also demonstrated that MCECs transduced with USP20-DN adenovirus increased angiogenic sprouting compared to the GFP control, whereas MCECs transduced with USP20-WT adenovirus decreased angiogenic sprouting ( xref )."

sparser
"We detected significantly more NFκB activity in cells expressing USP20-DN and USP20-S334D than in cells expressing USP20, USP20-S334A, and GFP ( xref – xref )."

sparser
"Similarly, NFκB activity is elevated in the MCECs transduced with recombinant AVs encoding USP20-DN and USP20-S334D mutants ( xref )."

sparser
"Vascular smooth muscle cell-specific expression of dominant-negative-USP20 (USP20-DN) increases vascular inflammation, NFκB activation, and atherosclerotic lesions in Ldlr −/− mice xref ."
USP20 affects RETREG1
| 13
USP20 deubiquitinates RETREG1.
| 10
USP20 deubiquitinates RETREG1. 10 / 10
| 10

reach
"Additionally, denaturing immunoprecipitation experiments provided evidence that USP20 could still deubiquitinate RETREG1 even when its RHD domain or the C-terminal domain was deleted (Figure S5E)."

reach
"Deubiquitination of RETREG1 by USP20 promotes its binding with LC3B and consequently enhances reticulophagy."

reach
"We then investigated the potential role of VAPs in mediating the deubiquitination of RETREG1 by USP20."

reach
"Surprisingly, even in the absence of the USP20 FFAT motifs, we observed that USP20 was still able to bind to and deubiquitinate RETREG1 (Figure S6A-B)."

reach
"Our study demonstrates that USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1 to facilitate reticulophagy."

reach
"During this stage, USP20 deubiquitinates RETREG1, stabilizing it and facilitating the recruitment of VAPs, along with early autophagy-related proteins, to the ER subdomain for the initiation of autophagy."

reach
"USP20 specifically mediates the deubiquitination of the reticulophagy receptor RETREG1."

reach
"The results indicated that RETREG1 is specifically deubiquitinated by USP20, as the ubiquitination level of RETREG1 significantly decreased under conditions of USP20 overexpression (Figure S4A)."

reach
"These findings indicate that USP20 participates in the regulation of RETREG1 degradation through the proteasome pathway.Next, we sought to identify the specific sites where USP20 deubiquitinates RETREG1."

reach
"Furthermore, USP20 was still able to effectively deubiquitinate RETREG1 even in the presence of these mutations."
USP20 binds RETREG1.
| 2
| 2

reach
"Moreover, the immunoprecipitation assay demonstrated an interaction between MYC-USP20 and FLAG-RETREG1, indicating their potential binding capability (Figure 3D)."

reach
"In addition, the endogenous interaction between USP20 and RETREG1 was confirmed through immunoprecipitation experiments using antibodies against RETREG1 (Figure 3E)."
USP20 activates RETREG1.
| 1
USP20 activates RETREG1. 1 / 1
| 1

reach
"Remarkably, our results indicated that overexpression of USP20 significantly enhanced the stability of RETREG1, whereas knockdown of USP20 led to reduced RETREG1 protein stability (Figure 3G-J)."
| 13

reach
"USP20 may promote tumor metastasis and is associated with immune infiltration and drug resistance in CRC."

reach
"In the metastasis assay, USP20 knockdown reduced lung metastasis, and this was reversed by YAP1 overexpression (Fig. 5G)."

reach
"USP20 also promotes metastasis of CRC cells and is closely correlated with several immune checkpoint genes, including ADORA2A (adenosine A2a receptor), CD160 (cluster of differentiation 160), CD27 (cluster of differentiation 27), and TNFRSF25 (TNF receptor superfamily member 25)[79]."

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."

reach
"USP20 enhances CRC cells metastasis and is associated with immune infiltration, immune checkpoints, and chemotherapy resistance."

reach
"Some researchers found that USP20 promotes the metastasis of breast cancer (13)."

reach
"USP20 promotes the metastasis of CRC cells."

reach
"Research has shown that USP20, a deubiquitinase, promotes breast cancer metastasis by stabilizing SNAI2 [39]."

reach
"Moreover, a study reported that USP20 promotes breast cancer metastasis by stabilizing the metastasis-promoting transcription factor SNAI2 (snail family transcriptional repressor 2) [52]."

reach
"In addition, USP20 can promote breast cancer metastasis by stabilizing SNAI2 [32]."
USP20 affects HMGCR
| 10 3
USP20 activates HMGCR.
| 6
USP20 activates HMGCR. 6 / 6
| 6

reach
"This licenses USP20’s recruitment into the HMGCR complex and antagonizes its degradation, thereby stabilizing HMGCR and promoting cholesterol synthesis [133]."

reach
"Inhibition of USP20 can promote HMGCR degradation and decrease lipid synthesis."

reach
"Besides decreasing HMGCR and lipid biosynthesis, knock-out or silencing of Usp20 led to the increase of UCP1 and energy expenditure in BAT and iWAT (Figs."

reach
"Owing to the chronic activity of p-mTOR, more USP20-mediated stabilization of HMGCR was observed in the HFD group than in the control group upon re-feeding."

reach
"This observation suggests that alternative regulatory mechanisms may be involved in mediating TBBPA’s effects on hepatic lipid metabolism.Our findings reveal a previously unrecognized mechanism underlying TBBPA-induced dyslipidemia, centered on post-transcriptional regulation of cholesterol synthesis through USP20-mediated HMGCR stabilization."

reach
"By contrast, USP20 inhibition promotes HMGCR degradation, reduces lipid biosynthesis, enhances succinate production, and promotes heat generation."
USP20 binds HMGCR.
| 1 3
| 1 3

reach
"HMGCR recruited phosphorylated USP20 to form the HMGCR-USP20 complex, which protected HMGCR from proteasome-mediated degradation [177]."

sparser
"HMGCR recruited phosphorylated USP20 to form the HMGCR-USP20 complex, which protected HMGCR from proteasome-mediated degradation [ xref ]."

sparser
"By doing so, mTORC1 enhanced the USP20-HMGCR interaction and prevented HMGCR degradation."

sparser
"Furthermore, Lu XY et al. reported that mTORC1 might control cholesterol synthesis by regulating the USP20-HMGCR axis [53] , emphasizing the importance of mTORC1 activation."
USP20 inhibits HMGCR.
| 1
USP20 inhibits HMGCR. 1 / 1
| 1

reach
"Lu et al. demonstrated that mechanistic target of rapamycin complex 1 phosphorylated USP20 at serine 132 and serine 134 and then increased the stability of HMG-CoA reductase (HMGCR), the rate-limiting enzyme in the cholesterol biosynthetic pathway [43]."
USP20 increases the amount of HMGCR.
| 1
USP20 increases the amount of HMGCR. 1 / 1
| 1

reach
"Silence of Usp20 inhibit statin-induced upregulation of HMGCR expression."
USP20 deubiquitinates HMGCR.
| 1
Phosphorylated USP20 deubiquitinates HMGCR. 1 / 1
| 1

reach
"In contrast to ubiquitination, HMGCR is deubiquitinated by mTORC1-phosphorylated USP20, which preferentially hydrolyzes K48 and K63 linkages, and stabilized HMGCR increases cholesterol synthesis in the feeding state (Lu et al., 2020)."
USP20 affects Flag
| 13
| 13

sparser
"Recent studies reported that the posttranslational modifications of DUBs can influence their stability and deubiquitinase activity. xref , xref , xref We then ectopic expressed FlagUSP20 in HCC cells and performed immunoprecipitation (IP)‐based MS."

sparser
"Next, we verified the binding between USP20 and STAT3 by co‐transfection of FlagUSP20 and His‐STAT3 plasmids into NIH3T3 cells, with no significant binding detected in the cells transfected with FlagUSP20 or His‐STAT3 and IgG control group. (Figure  xref )."

sparser
"To verify whether USP20 targeted HSPA2, HepG2 cells were transfected with Flag-USP20 cDNA and USP20 short hairpin RNA."

sparser
"Interestingly, we discovered that ATR, a key player in replication stress signaling, xref was copurified with FlagUSP20 (Figure xref )."

sparser
"As shown in Fig. 4 H, HA-PTEN was co-immunoprecipitated with Flag-USP20 using anti-Flag antibody only when both plasmids were transfected."

sparser
"Overexpression of FlagUSP20 did not increase the STAT3 protein level in NIH3T3 cells ( Figure   xref )."

sparser
"Immunoblotting suggested that co-immunoprecipitation occurred when Flag-USP20 and Myc-HSPA2 were co-expressed in HEK-293T cells ( xref )."

sparser
"After co‐transfection of these mutants with FlagUSP20 plasmid in HEK293T cells, result showed that the absence of amino acids 130–320, which constitute the CCD domain, disrupted the binding between STAT3 and USP20 (Figure xref , Supporting Information)."

sparser
"As shown in xref , Myc-HSPA2 was ubiquitinated in the presence of HA-ubiquitin, and the co-expression of Flag-USP20 reduced the ubiquitination of Myc-HSPA2."

sparser
"A consistent trend was observed in USP20 ‐NIH/3 T3 cells transferred with FlagUSP20 and FlagUSP20C154A (Figures  xref and xref )."
Tmc1 affects USP20
| 13
| 13

sparser
"The angiogenic sprouting of MCECs is diminished by the expression of WT and phospho-resistant USP20-S334A, while it is exacerbated by USP20-DN and phospho-mimetic USP20-S334D."

sparser
"To test the role of USP20 and its phosphorylation status in EC sprouting, we performed the spheroid-based sprouting assay using MCECs transduced with recombinant adenoviruses, expressing USP20-WT, USP20-DN, USP20-S334A, and USP20-S334D ( xref – xref )."

sparser
"NFκB activity was augmented in MCECs with overexpression of USP20-DN but diminished with exogenous expression of USP20 WT ( xref )."

sparser
"We therefore evaluated the effect of USP20 (USP20-WT), inactive USP20 (USP20-dominant negative or USP20-DN), phospho-resistant USP20 (USP20-S334A), and phospho-mimetic USP20 (USP20-S334D) on NFκB activation triggered by IL-1β."

sparser
"Assessed by scratch-wound healing and spheroid assays, migration and angiogenesis of MCECs, respectively, were (a) increased by USP20-DN or USP20(S334D), and (b) decreased by USP20-WT or USP20(S334A)."

sparser
"USP20-WT and USP20-S334A transduced MCECs formed weak or no sprouts from spheroids compared with the GFP-treated MCECs ( xref ), whereas angiogenic sprouting was significantly increased in USP20-DN and USP20-S334D transduced MCECs compared with the GFP-treated MCECs ( xref )."

sparser
"Data from our in vitro spheroid assay also demonstrated that MCECs transduced with USP20-DN adenovirus increased angiogenic sprouting compared to the GFP control, whereas MCECs transduced with USP20-WT adenovirus decreased angiogenic sprouting ( xref )."

sparser
"We detected significantly more NFκB activity in cells expressing USP20-DN and USP20-S334D than in cells expressing USP20, USP20-S334A, and GFP ( xref – xref )."

sparser
"Similarly, NFκB activity is elevated in the MCECs transduced with recombinant AVs encoding USP20-DN and USP20-S334D mutants ( xref )."

sparser
"Vascular smooth muscle cell-specific expression of dominant-negative-USP20 (USP20-DN) increases vascular inflammation, NFκB activation, and atherosclerotic lesions in Ldlr −/− mice xref ."
STING1 affects USP20
3 | 10
3 | 6

No evidence text available

sparser
"The present study demonstrated that USP20 interacted with STING and facilitated its degradation, consequently alleviating the myocardial inflammation and improving ventricular remodeling and heart failure induced by diabetes."

sparser
"Domain‐focused Co‐IP analyzes revealed that USP20 binds exclusively to STING via its catalytic USP domain, a conclusion supported by the complete loss of interaction in the catalytic USP domain mutant (Figure  xref )."

sparser
"In mechanism, USP20 directly binds to STING and promotes its degradation through the autophagy pathway by deubiquitinating p62 via its active site C154, thereby alleviating the myocardial inflammation and improving ventricular remodeling and heart failure induced by diabetes."

No evidence text available

sparser
"USP20 directly binds to STING and promotes the degradation of STING through the autophagy pathway by deubiquitinating p62 via its active site C154, thereby alleviating myocardial inflammation and improving ventricular remodeling and heart failure induced by diabetes."

No evidence text available

sparser
"Notably, STING emerged as the only overlapping candidate substrate across all three groups (Figure  xref ), prompting us to investigate whether an interaction exists between USP20 and STING."

sparser
"We then examined the specific domains through which USP20 interacts with STING."
| 4

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."
PKA affects USP20
| 5 6 1
PKA phosphorylates USP20.
| 5 5 1
PKA phosphorylates USP20. 8 / 8
| 4 4

sparser
"PKA-dependent phosphorylation of the DUB USP20 was recently shown to perturb post-endocytic trafficking of the β-andregenic receptor under cellular stress conditions."

reach
"Phosphorylation of USP20 by protein kinase A has been reported to regulate β2 adrenergic receptors intracellular trafficking (Yu et al., 2019)."

reach
"Most recently, it was described that upon physiological stress, USP20 is phosphorylated by protein kinase A and this process regulates trafficking of beta 2 AR to autophagosomes [XREF_BIBR]."

sparser
"Phosphorylation of USP20 by protein kinase A has been reported to regulate β2 adrenergic receptors intracellular trafficking ( xref )."

sparser
"This process requires the protein kinase A-dependent phosphorylation and deactivation of the deubiquitinating enzyme USP20, allowing for the ubiquitination of β2AR to persist and act as a sorting signal to autophagosomes [ xref ]."

reach
"Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of beta2 adrenergic receptors to autophagosomes during physiological stress."

reach
"PKA dependent phosphorylation of the DUB USP20 was recently shown to perturb post-endocytic trafficking of the beta-andregenic receptor under cellular stress conditions."

sparser
"Most recently, it was described that upon physiological stress, USP20 is phosphorylated by protein kinase A and this process regulates trafficking of β 2 AR to autophagosomes [ xref ]."
PKA phosphorylates USP20 on S333. 2 / 3
| 1 1

sparser
"Subsequently, they found that protein kinase A phosphorylates USP20 on serine 333, inhibits the trafficking of the substrate β2AR (β2 adrenergic receptors) and decreases degradation via autophagosomes [ xref ]."

reach
"Subsequently, they found that protein kinase A phosphorylates USP20 on serine 333, inhibits the trafficking of the substrate β2AR (β2 adrenergic receptors) and decreases degradation via autophagosomes [41]."
PKA phosphorylates USP20. 1 / 1
| 1

rlimsp
"Most recently, it was described that upon physiological stress, USP20 is phosphorylated by protein kinase A and this process regulates trafficking of β2AR to autophagosomes [112]."
PKA inhibits USP20.
| 1
PKA inhibits USP20. 1 / 1
| 1

sparser
"Concomitant inhibition of the deubiquitinase USP20 (Ubiquitin-specific-processing protease 20) by PKA favors ubiquitylation and degradation of the receptor by NEDD4 ( xref ; xref )."
Flag affects USP20
| 13
| 13

sparser
"Recent studies reported that the posttranslational modifications of DUBs can influence their stability and deubiquitinase activity. xref , xref , xref We then ectopic expressed FlagUSP20 in HCC cells and performed immunoprecipitation (IP)‐based MS."

sparser
"Next, we verified the binding between USP20 and STAT3 by co‐transfection of FlagUSP20 and His‐STAT3 plasmids into NIH3T3 cells, with no significant binding detected in the cells transfected with FlagUSP20 or His‐STAT3 and IgG control group. (Figure  xref )."

sparser
"To verify whether USP20 targeted HSPA2, HepG2 cells were transfected with Flag-USP20 cDNA and USP20 short hairpin RNA."

sparser
"Interestingly, we discovered that ATR, a key player in replication stress signaling, xref was copurified with FlagUSP20 (Figure xref )."

sparser
"As shown in Fig. 4 H, HA-PTEN was co-immunoprecipitated with Flag-USP20 using anti-Flag antibody only when both plasmids were transfected."

sparser
"Overexpression of FlagUSP20 did not increase the STAT3 protein level in NIH3T3 cells ( Figure   xref )."

sparser
"Immunoblotting suggested that co-immunoprecipitation occurred when Flag-USP20 and Myc-HSPA2 were co-expressed in HEK-293T cells ( xref )."

sparser
"After co‐transfection of these mutants with FlagUSP20 plasmid in HEK293T cells, result showed that the absence of amino acids 130–320, which constitute the CCD domain, disrupted the binding between STAT3 and USP20 (Figure xref , Supporting Information)."

sparser
"As shown in xref , Myc-HSPA2 was ubiquitinated in the presence of HA-ubiquitin, and the co-expression of Flag-USP20 reduced the ubiquitination of Myc-HSPA2."

sparser
"A consistent trend was observed in USP20 ‐NIH/3 T3 cells transferred with FlagUSP20 and FlagUSP20C154A (Figures  xref and xref )."
USP20 affects PINK1
| 12
| 12

sparser
"We subsequently explored whether PINK1 directly binds to USP20 in the absence or presence of CCCP treatment."

sparser
"Cell lysates were immunoprecipitated with anti-Flag antiserum, and immunoblotting with anti-Myc antibodies revealed that ectopically expressed USP20 binds to PINK1 (Fig.  xref A)."

sparser
"Co-IP analysis revealed that ectopically expressed PINK1 interacts with USP20 in the basal state."

sparser
"The model confidence score (pLDDT) of AlphaFold3-prediction and the average distance between both the N-lobe and C-lobe of PINK1 and USP20 (approximately 3–4 Å) supports a direct physical interaction between PINK1 and USP20 (Supple Fig. xref )."

sparser
"To validate the endogenous interaction, we additionally conducted a Proximity Ligation Assay (PLA), which demonstrated that endogenous PINK1 interacts with USP20 in the cytosol (Fig.  xref H)."

sparser
"Overall, our data indicates that USP20 binds to PINK1 in the cytosol of mammalian cells."

sparser
"PINK1 Kinase Domain Interacts with the Catalytic Domain of USP20."

sparser
"Future studies in neuronal and in vivo models are warranted to validate these findings and further elucidate the physiological and pathological relevance of the USP20PINK1 axis."

sparser
"PINK1 interacts with USP20 when the intact kinase domain of PINK1 is present (Fig.  xref D)."

sparser
"However, deletion of the N-lobe of the kinase domain abolished the PINK1 binding to USP20."
USP20 affects NFkappaB
| 1 8 2
USP20 inhibits NFkappaB.
| 1 5 1
| 1 5 1

reach
"USP18 deficiency or knockdown of USP20 resulted in enhanced K48 linked ubiquitination and accelerated degradation of STING, and impaired activation of IRF3 and NF-kappaB as well as induction of downstream genes after infection with DNA virus HSV-1 or transfection of various DNA ligands."

eidos
"Ubiquitin-specific peptidase 20 ( USP20 ) reduces NFkappaB activation triggered by pro-inflammatory stimuli , and USP20 activity attenuates atherosclerosis in mice ."

reach
"XREF_BIBR This study showed that USP18 or USP20 deficiency significantly inhibited HSV-I or cytosolic DNA activation of both NF-kappaB and IRF3, and type-I IFN and proinflammatory cytokine expression."

reach
"Ubiquitin-specific peptidase 20 (USP20) reduces NFkappaB activation triggered by proinflammatory stimuli, and USP20 activity attenuates atherosclerosis in mice."

reach
"Silencing USP20 in SMCs with siRNA (small interfering RNA) augmented NFkappaB activation by = ~ 50% in response to either TNF or IL-1beta (interleukin-1beta)."

reach
"XREF_BIBR USP20 inhibits NF-kappaB activation via Toll like receptor (TLR) 4, XREF_BIBR IL-1R, and the TNF XREF_BIBR inflammatory pathway in VSMCs."

sparser
"USPs are the most abundant DUBs, with approximately 60 proteases in humans. xref USP20 inhibits NF-κB activation via Toll-like receptor (TLR)4, xref IL-1R, and the TNF xref inflammatory pathway in VSMCs."
USP20 activates NFkappaB.
| 3
| 3

reach
"Our findings reveal novel mechanisms regulating IL-1beta-induced proinflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFkappaB activation, SMC inflammation, and neointimal hyperplasia."

reach
"USP20 is downregulated in HTLV-1 infected cell lines, presumably to promote high levels of NF-kappaB activation, although the exact mechanism is unclear [XREF_BIBR]."

reach
"Coimmunoprecipitation experiments revealed that USP20 associates with several components of the TNFR1 (TNF receptor-1) signaling pathway, including RIPK1 (receptor interacting protein kinase 1), a critical checkpoint in TNF induced NFkappaB activation and inflammation."
USP20 binds NFkappaB.
| 1

sparser
"Co-immunoprecipitation showed that USP20 interacts with several NF-κB signaling components, including receptor-interacting protein kinase 1 (RIPK1) [ xref ]."
PINK1 affects USP20
| 12
| 12

sparser
"We subsequently explored whether PINK1 directly binds to USP20 in the absence or presence of CCCP treatment."

sparser
"Cell lysates were immunoprecipitated with anti-Flag antiserum, and immunoblotting with anti-Myc antibodies revealed that ectopically expressed USP20 binds to PINK1 (Fig.  xref A)."

sparser
"Co-IP analysis revealed that ectopically expressed PINK1 interacts with USP20 in the basal state."

sparser
"The model confidence score (pLDDT) of AlphaFold3-prediction and the average distance between both the N-lobe and C-lobe of PINK1 and USP20 (approximately 3–4 Å) supports a direct physical interaction between PINK1 and USP20 (Supple Fig. xref )."

sparser
"To validate the endogenous interaction, we additionally conducted a Proximity Ligation Assay (PLA), which demonstrated that endogenous PINK1 interacts with USP20 in the cytosol (Fig.  xref H)."

sparser
"Overall, our data indicates that USP20 binds to PINK1 in the cytosol of mammalian cells."

sparser
"PINK1 Kinase Domain Interacts with the Catalytic Domain of USP20."

sparser
"Future studies in neuronal and in vivo models are warranted to validate these findings and further elucidate the physiological and pathological relevance of the USP20PINK1 axis."

sparser
"PINK1 interacts with USP20 when the intact kinase domain of PINK1 is present (Fig.  xref D)."

sparser
"However, deletion of the N-lobe of the kinase domain abolished the PINK1 binding to USP20."
HIF1A affects USP20
3 | 3 5
3 | 3 5

reach
"The interaction between HIF-1alpha and VDU2 was shown using an immunoprecipitation assay."

sparser
"However, by using in vitro pull down assays with GST-HIF-1α (amino acid 530–826) and co-immunoprecipitation experiments in COS-7 cells it was shown that only VDU2 but not VDU1 could interact with HIF-1α (Li et al., xref )."

sparser
"In contrast to VDU2 which directly interacts with HIF-1α, USP28 forms a ternary complex with HIF-1α via its association with HIF-1α bound Fbw7 (Flügel et al., xref )."

sparser
"VDU2 can interact with HIF-1α, a tumor suppressor, to deubiquitinate and stabilize HIF-1α ( xref )."

reach
"Molecular and cellular experiments showed that VDU2 interacted with HIF-1alpha in order to induce the expression of HIF-1 downstream genes, such as VEGF, through the deubiquitination and stabilization of HIF-1alpha."

sparser
"VDU2 binds and de-ubiquitylates HIF-1α in a pVHL-dependent manner, hence salvaging it from proteasomal degradation."

sparser
"According to those findings, USP20 binds to and stabilizes HIF-1α."

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."

No evidence text available

No evidence text available
USP20 affects autophagy
| 11
USP20 activates autophagy.
| 10
| 10

reach
"USP20 promotes autophagy at multiple steps."

reach
"Our result found that silencing of USP20 could inhibit autophagy, not singly but in pairs, the overexpression of USP33, which is the homology of USP20, could deubiquitinate RALB at Lys 47, and then regulate assembly of the RALB, EXO84, and beclin1 complex that initiated autophagy."

reach
"These findings strongly indicate that knockdown of USP20 inhibits autophagy flux."

reach
"USP20 facilitates RETREG1-VAPB association and enhances early autophagy protein recruitment for reticulophagy."

reach
"Similarly, the kinase ULK1 is stabilized by USP20, which is necessary to initiate autophagy ."

reach
"On the contrary, autophagy activity was enhanced by the USP20 overexpression (XREF_FIG)."

reach
"While the deubiquitinating enzyme USP20 and STAMBP/AMSH promote autophagy initiation by stabilizing ULK1 [21, 46], USP24 was found to negatively regulate autophagy and the stability of ULK1 [47]."

reach
"Experimentally, small interfering RNA mediated knockdown of USP20 expression significantly promoted cell proliferation, accelerated G1-S phase transition and attenuated the autophagy activity."

reach
"Thus, whether the autophagy caused by USP20 could contribute to the inhibition of tumor growth is worthy of investigation in a further study."

reach
"USP20 induces autophagy by stabilizing ULK1 [41]."
USP20 inhibits autophagy.
| 1
| 1

reach
"USP20 stabilizes claspin and inhibits proliferation, G1–S cell cycle transition, and autophagy in gastric cancer cells [23] ."
USP20 affects CTNNB1
1 1 | 6 3
USP20 deubiquitinates CTNNB1.
1 | 3
USP20 deubiquitinates CTNNB1. 4 / 4
1 | 3

"Mechanistically, <span class="match term0">USP20</span> regulates the deubiquitination of <span class="match term1">BETA-CATENIN</span> to control its stability, thereby inducing proliferation, invasion and migration of cancer cells"

reach
"Moreover, knockdown of USP20 increases beta-catenin polyubiquitination, which enhances beta-catenin turnover and cell sensitivity to chemotherapy."

reach
"Importantly, the inhibition of β-catenin and Twist1 effectively suppresses the stemness induced by USP4.Previous studies have shown that USP8 and USP20 can deubiquitinate β-catenin, while DUB3 and USP51 target Twist1 [50–53]."

reach
"In addition, USP20 depletion promotes the polyubiquitination of β-catenin, which facilitates β-catenin turnover and increases cell sensitivity to chemotherapy [31]."
USP20 binds CTNNB1.
1 | 3
1 | 3

sparser
"Several ubiquitin-specific proteases (USPs), including USP2a [ xref ], USP39 [ xref ], USP47 [ xref ], and USP20 [ xref ] also directly bind to and deubiquitinate β-catenin in the cytoplasm, leading to elevated expression of Wnt target genes."

sparser
"To further investigate the role of the USP20-β-catenin axis in cancers, they detected the protein and mRNA levels of USP20 and β-catenin in colon cancer and other cancer cell lines, including osteosarcoma, cervical, breast, ovarian, and colorectal cancer cell lines [ xref ]."

No evidence text available

sparser
"Thus, the USP20-β-catenin axis may be an emerging therapeutic target in colon cancer."
USP20 activates CTNNB1.
| 3
USP20 activates CTNNB1. 3 / 3
| 3

reach
"USP20 and USP4 induce drug resistance in breast cancer and colorectal cancer cells by stabilizing β-catenin, the key modulator of the Wnt pathway [249, 250]."

reach
"Previous publications have identified β-catenin as an oncogene [62], prompting us to speculate that USP20 promotes tumorigenesis by regulating β-catenin stability."

reach
"USP20 and USP4 promote the stability of β-catenin, thus continuously activating the Wnt-β-catenin signaling pathway and mediating drug resistance in breast cancer and CRC ( Fig. 3 )."
USP20 affects CHEK1
| 7
USP20 activates CHEK1.
| 4
USP20 activates CHEK1. 4 / 8
| 4

reach
"USP20 promotes CHK1 activation in response to replication stress or UV radiation."

reach
"Taken together, these results indicate that USP20 promotes CHK1 activation in response to HU induced replication stress or UV irradiation."

reach
"Upon replication stress, ATR mediated phosphorylation of USP20 promotes dissociation of HERC2 from USP20, stabilizes USP20 and its association with CLASPIN, thus increasing CALSPIN stability and ensuring CHK1 activation."

reach
"To test if USP20 mediated stability of CLASPIN promotes CHK1 activation upon replication stress, we expressed wild-type FLAG-CLASPIN in USP20 depleted cells."
USP20 dephosphorylates CHEK1.
| 2
USP20 leads to the dephosphorylation of CHEK1. 2 / 2
| 2

reach
"Furthermore, depletion of USP20 can not further reduce Chk1 phosphorylation in Claspin knocking-down cells."

reach
"As shown in Figure XREF_FIG, depletion of USP20 in cells dramatically reduced Chk1 phosphorylation following DNA damage."
USP20 inhibits CHEK1.
| 1
USP20-C154S inhibits CHEK1. 1 / 1
| 1

reach
"Concomitantly, expression of the catalytically inactive mutant FLAG USP20 (C154S) attenuated HU induced CHK1 activation."
GroEL2 affects USP20
| 3 7
| 3 3

reach
"Interestingly, we observed a stronger interaction between VDU2 and hSP56 than between VDU1 and hSP56 (XREF_FIG B)."

sparser
"We previously identified pVHL-interacting deubiquitinating enzyme 1 (VDU1) as a protein interacting with hSP56 xref , thus extended this finding to examine whether hSP56 interacts also with VDU2, a closely related isoform of VDU1."

reach
"HSP56 interacts with both VDU1 and VDU2."

sparser
"Interestingly, we observed a stronger interaction between VDU2 and hSP56 than between VDU1 and hSP56 ( xref B)."

sparser
"These results suggest that the interaction of hSP56 with VDU2 is relevant."

reach
"We previously identified pVHL interacting deubiquitinating enzyme 1 (VDU1) as a protein interacting with hSP56 XREF_BIBR, thus extended this finding to examine whether hSP56 interacts also with VDU2, a closely related isoform of VDU1."
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
| 5 1

reach
"Notably, mechanistic analysis indicated that USP20 directly interacted with PTEN, thereafter reducing inflammatory response in microglial cells and mitigating neuronal death in cortical neurons upon O[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Thus, USP20 inhibits SMC inflammation in response to the atherogenic cytokine IL-1β, as it does to the atherogenic activation of TLR4. xref "

reach
"Smooth muscle cell (SMC) USP20 activity suppresses vascular inflammation, as demonstrated in the context of carotid endothelial denudation in nonatherogenic mice (3) and atherosclerosis in Ldlr mice (4)."

reach
"Preventing USP20 phosphorylation on Ser334 attenuates vascular inflammation."

reach
"USP20 (Ubiquitin Specific Protease 20) Inhibits TNF (Tumor Necrosis Factor)-Triggered Smooth Muscle Cell Inflammation and Attenuates Atherosclerosis."

reach
"In vivo, specific overexpression of USP20 in VSMCs significantly reduces vascular inflammation and ameliorates atherosclerosis."
| 4

reach
"Coimmunoprecipitation experiments revealed that USP20 associates with several components of the TNFR1 (TNF receptor-1) signaling pathway, including RIPK1 (receptor interacting protein kinase 1), a critical checkpoint in TNF induced NFkappaB activation and inflammation."

reach
"Phosphorylation of USP20 on Ser334 by IRAK1 promotes IL-1beta-evoked signaling in vascular smooth muscle cells and vascular inflammation."

reach
"Our findings reveal novel mechanisms regulating IL-1beta-induced proinflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFkappaB activation, SMC inflammation, and neointimal hyperplasia."

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects groEL2
| 3 7
| 3 3

reach
"Interestingly, we observed a stronger interaction between VDU2 and hSP56 than between VDU1 and hSP56 (XREF_FIG B)."

sparser
"We previously identified pVHL-interacting deubiquitinating enzyme 1 (VDU1) as a protein interacting with hSP56 xref , thus extended this finding to examine whether hSP56 interacts also with VDU2, a closely related isoform of VDU1."

reach
"HSP56 interacts with both VDU1 and VDU2."

sparser
"Interestingly, we observed a stronger interaction between VDU2 and hSP56 than between VDU1 and hSP56 ( xref B)."

sparser
"These results suggest that the interaction of hSP56 with VDU2 is relevant."

reach
"We previously identified pVHL interacting deubiquitinating enzyme 1 (VDU1) as a protein interacting with hSP56 XREF_BIBR, thus extended this finding to examine whether hSP56 interacts also with VDU2, a closely related isoform of VDU1."
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
USP20 affects ferroptosis
| 10
USP20 inhibits ferroptosis.
| 5
| 5

reach
"Pharmacological inhibition or knockdown of USP20 in HCC cells triggered ferroptosis and reversed OXA resistance both in vitro and in vivo."

reach
"Ubiquitin specific peptidase 20 (USP 20) maintains the stability of SLC 7A 11 protein by removing K48 linked polyubiquitination of SLC 7A 11 protein at K30 and K37, inhibits the occurrence of ferroptosis in liver cancer, and increases the tolerance to oxaliplatin (64)."

reach
"These results indicated that inhibition of USP20 by GSK2643943A could induce ferroptosis of HCC in vivo."

reach
"USP20 contributes OXA resistance and suppress ferroptosis in HCC."

reach
"Pharmacological inhibition or knockdown of USP20 triggered ferroptosis and increased the sensitivity of HCC cells to OXA both in vitro and in vivo."
USP20 activates ferroptosis.
| 5
| 5

reach
"Therefore, targeting USP20 may mitigate the development of drug resistance and promote ferroptosis of HCC in patients receiving chemotherapy.4 METHODS."

reach
"We observed that USP20 depletion reduced the cell viability upon the treatment of erastin, which could be rescued by Ferr‐1, suggesting that the mechanism is specific in cell ferroptosis (Figures 2F, G and S2G)."

reach
"Therefore, targeting USP20 may mitigate the development of drug resistance and promote ferroptosis of HCC in patients receiving chemotherapy."

reach
"USP20 depletion also reduced the cell viability upon the treatment of erastin, which could be recovered by Ferr‐1, suggesting that the mechanism is specific in cell ferroptosis."

reach
"Targeting USP20 may mitigate the development of drug resistance and promote ferroptosis of HCC in patients receiving chemotherapy.2 RESULTS."
USP20 affects USP20
| 1 9
USP20 phosphorylates USP20.
| 4
USP20 phosphorylates USP20 on S333. 3 / 3
| 3

reach
"However, incubation of this purified USP20 with IRAK1 increased human USP20 Ser333 phosphorylation >2-fold, as detected by our phospho-USP20(Ser333) IgG (Fig. 9, A and B)."

reach
"Thus, IRAK1 can phosphorylate USP20 on Ser333 (of the human USP20 sequence) in preparations of purified proteins.Does IRAK1 phosphorylate mouse USP20 on Ser334 in intact SMCs?"

reach
"Thus, IRAK1 can phosphorylate USP20 on Ser333 (of the human USP20 sequence) in preparations of purified proteins.Does IRAK1 phosphorylate mouse USP20 on Ser334 in intact SMCs?"
USP20 phosphorylates USP20 on S132. 1 / 1
| 1

reach
"Lu et al. demonstrated the post-prandial increase in insulin and the glucose concentration stimulates mTORC1 to phosphorylate the deubiquitylase ubiquitin-specific peptidase 20 (USP20) at S132 and S134, which is recruited to the HMGCR complex and antagonizes its degradation (Lu et al., 2020)."
USP20 inhibits USP20.
| 3
USP20 inhibits USP20. 3 / 3
| 3

reach
"Our findings reveal novel mechanisms regulating IL-1beta-induced proinflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFkappaB activation, SMC inflammation, and neointimal hyperplasia."

reach
"Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

reach
"Phosphorylation of USP20 on Ser334 substantially attenuates the anti-inflammatory activity of USP20-WT, whether USP20-WT expression is physiologic or supraphysiologic."
USP20 activates USP20.
| 1 1
USP20 activates USP20. 2 / 2
| 1 1

eidos
"] Although deubiquitinating STING alone , USP20 can be recruited by USP18 , thus causing USP20 to remove K48-linked polyubiquitin chains more efficiently , even at a low dose ."

reach
"These suggest that ATR mediated phosphorylation of USP20 promotes dissociation of USP20 from HERC2, and this dissociation would stabilize CLASPIN and promote CHK1 activation."
USP20 increases the amount of USP20.
| 1
USP20 increases the amount of USP20. 1 / 1
| 1

reach
"RT-qPCR and western blotting assays confirmed that local injection of LV-USP20 dramatically increased USP20 expression in brain tissues of mice compared to the mice injected with LV-Vec ( Fig. 1 H and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects USP18
| 8
| 4

sparser
"In 2016, Zhang et al. [ xref ] reported that USP18 interacts with the deubiquitinase USP20 and recruits it to stimulator of interferon genes (STING)."

sparser
"USP18-USP20 mediated the accumulation of STING that regulates oHSV-1 T1012G viral lytic replication in SCC9 cells."

sparser
"All the above results indicate that USP18-USP20 mediated the accumulation of STING function with limited oHSV-1 T1012G virus yields in SCC9 cells."

sparser
"Immunoprecipitation revealed that STING, USP18 and USP20 are arranged as USP20-USP18-STING, but both USP20 and USP18 were associated with the N-terminus of STING [ xref ]."
| 4

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."
USP20 affects RAD17
4 1 | 3 2
USP20 binds RAD17.
4 | 2
4 | 2

No evidence text available

No evidence text available

No evidence text available

sparser
"We demonstrate that USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure."

sparser
"ATM/ATR-mediated phosphorylation of USP20 does not affect its DUB activity; rather, this USP20 phosphorylation enhances the association of USP20 with Rad17 and thereby enhances USP20-mediated deubiquitination of Rad17 ( xref )."

No evidence text available
USP20 increases the amount of RAD17.
| 2
Modified USP20 increases the amount of RAD17. 1 / 1
| 1

reach
"It was also noted that inhibition of USP20 expression decreased RAD17 protein levels."
USP20 increases the amount of RAD17. 1 / 1
| 1

reach
"Consistent with this report, we found knockdown of USP20 in cells decreased Rad17 levels."
USP20 deubiquitinates RAD17.
1 | 1
USP20 deubiquitinates RAD17. 2 / 2
1 | 1

"Similar to Chk1 and <span class="match term1">Rad17</span>, which enhance recombinational repair of collapsed replication forks, we demonstrate that <span class="match term0">USP20</span> depletion impairs DNA double strand break repair by homologous recombination."

reach
"ATM/ATR-mediated phosphorylation of USP20 does not affect its DUB activity; rather, this USP20 phosphorylation enhances the association of USP20 with Rad17 and thereby enhances USP20-mediated deubiquitination of Rad17 (41)."
USP20 affects MAPK6
2 1 | 5 2
USP20 binds MAPK6.
2 | 2 2
2 | 2 2

sparser
"They demonstrated that USP20 overexpression could enhance HeLa cell migration, and they further examined the impact of the USP20-ERK3 axis in regulating the migration of breast cancer cell lines [ xref ]."

sparser
"These results indicate that the USP20-ERK3 axis can promote the migration of breast cancer cell lines, suggesting that USP20 may play an important role in the tumorigenesis of breast cancer."

reach
"USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."

No evidence text available

reach
"The interaction between USP20 and ERK3 was demonstrated at endogenous levels of both the proteins in cultured cells."

No evidence text available
USP20 deubiquitinates MAPK6.
1 | 3
USP20 deubiquitinates MAPK6. 4 / 4
1 | 3

reach
"One study showed that USP20 deubiquitinates ERK3 both in vitro and in cultured cells, leading to its stabilization [35]."

reach
"USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."

reach
"A later study confirmed USP20 de-ubiquitinates ERK3 to increase stability during C2C12 myoblast differentiation [35]."

"<span class="match term0">USP20</span> interacts with and deubiquitinates <span class="match term1">ERK3</span> both in vitro and in intact cells"
USP18 affects USP20
| 8
| 4

sparser
"In 2016, Zhang et al. [ xref ] reported that USP18 interacts with the deubiquitinase USP20 and recruits it to stimulator of interferon genes (STING)."

sparser
"USP18-USP20 mediated the accumulation of STING that regulates oHSV-1 T1012G viral lytic replication in SCC9 cells."

sparser
"All the above results indicate that USP18-USP20 mediated the accumulation of STING function with limited oHSV-1 T1012G virus yields in SCC9 cells."

sparser
"Immunoprecipitation revealed that STING, USP18 and USP20 are arranged as USP20-USP18-STING, but both USP20 and USP18 were associated with the N-terminus of STING [ xref ]."
| 4

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."
USP20 affects HYCC1
| 9
USP20 activates HYCC1.
| 6
USP20 activates HYCC1. 6 / 6
| 6

reach
"Targeting USP20 may mitigate the development of drug resistance and promote ferroptosis of HCC in patients receiving chemotherapy.2 RESULTS."

reach
"Huh‐7, Hep3B, and HCC primary cell line were treated with increasing concentration of a glutamate–cystine antiporter system Xc− inhibitor (erastin), and found that USP20 depletion inhibited the viability of HCC cells, and cells depleted with USP20 were more sensitive to ferroptosis induced by erastin treatment (Figures 2A–C and S2E)."

reach
"Therefore, targeting USP20 may mitigate the development of drug resistance and promote ferroptosis of HCC in patients receiving chemotherapy.4 METHODS."

reach
"We observed that USP20 depletion remarkably inhibited the oncosphere formation of Huh‐7, Hep3B and HCC primary cells (Figures S2K, S5A, andB)."

reach
"We also observed that knockdown or pharmacologically inhibition of USP20 could significantly reduce the proliferation and viability of HCC cells, which was consistent with previous studies."

reach
"Therefore, targeting USP20 may mitigate the development of drug resistance and promote ferroptosis of HCC in patients receiving chemotherapy."
USP20 inhibits HYCC1.
| 2
USP20 inhibits HYCC1. 2 / 2
| 2

reach
"We then stably expressed USP20 WT, S132A/368A, and S132D/S368D in HCC primary cell line (from sample T7 with low USP20 expression) to examine the functions of USP20 phosphorylation in HCC cells,. Overexpression the wild type and S132D/S368D of USP20 decreased the sensitivity of the HCC primary cell line to OXA, while USP20 S132A/368A almost had no effect (Figure S8)."

reach
"These results indicated that inhibition of USP20 by GSK2643943A could induce ferroptosis of HCC in vivo."
USP20 decreases the amount of HYCC1.
| 1
USP20 decreases the amount of HYCC1. 1 / 1
| 1

reach
"Consistent with the results identified above, USP20 WT and S132D/S368D reduced cell sensitivity to erastin and increased the GSH levels, while ferrous iron levels decreased and lipid ROS levels of HCC cells (Figures 6H–L)."
USP20 affects CD274
| 6 3
USP20 increases the amount of CD274.
| 3
USP20 increases the amount of CD274. 3 / 3
| 3

reach
"After verifying its depletion and efficiency, we found that USP20 knockdown downregulated the expression of PD-L1 (Fig. 3E, F)."

reach
"USP20 overexpression rescued the reduction in PD-L1 expression after TINCR knockdown (Fig. 3I, J)."

reach
"In addition, USP20 overexpression had no influence on PD-L1 mRNA levels, but upregulated PD-L1 protein levels (Fig. 3G, H)."
USP20 binds CD274.
| 3
| 3

sparser
"Wang et al. demonstrated that USP20 directly interacts with PD-L1 [ xref ]."

sparser
"A recent study demonstrated that USP20 interacts with PD-L1 and deubiquitinates it, which can be regulated by a long non-coding RNA, tissue differentiation inducing non-protein coding RNA (TINCR)."

sparser
"Under physiological conditions, USP20PD-L1 interactions were validated using endogenous immunoprecipitation (Fig. xref )."
USP20 deubiquitinates CD274.
| 2
USP20 deubiquitinates CD274. 2 / 2
| 2

reach
"Consequently, USP20 deubiquitinates PD-L1, increasing BC cell resistance to PD-L1 inhibitors [213]."

reach
"USP20 overexpression inhibits PD-L1 ubiquitination."
USP20 ubiquitinates CD274.
| 1
USP20 leads to the ubiquitination of CD274. 1 / 1
| 1

reach
"USP20 depletion induced an increase in ubiquitinated PD-L1 in vivo, which was largely reduced after the rescue of USP20 (Fig. 3L)."
TRAF6 affects USP20
4 | 5
4 | 5

sparser
"The serine/threonine kinase IRAK1 is a key mediator of TRAF6 activation downstream of TLR4 and the IL-1R. Therefore, IRAK1 could not only promote TRAF6 activation but also prevent TRAF6 inactivation (deubiquitination)—if, indeed, IRAK1 phosphorylates USP20 on Ser334 (and thereby reduces the association of USP20 with TRAF6)."

No evidence text available

sparser
"Thus, it seems that preventing USP20 phosphorylation on Ser334 augments the association of USP20 with its intracellular substrate TRAF6, even though it has no effect on the association of USP20 with the idealized substrate Ub-VME ( xref , A and B )."

sparser
"Thus, IRAK1 not only transduces IL-1R signaling ( xref ) but also prevents its termination: phosphorylation of USP20 on Ser334 reduces USP20’s binding to TRAF6 and thereby diminishes (a) USP20-mediated deubiquitination/deactivation of TRAF6 and (b) consequent attenuation of downstream NFκB activation."

sparser
"Our findings reveal novel mechanisms regulating IL-1β-induced proinflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFκB activation, SMC inflammation, and neointimal hyperplasia."

No evidence text available

No evidence text available

sparser
"Together, these results suggest that IRAK1 inhibits USP20-mediated deubiquitination of TRAF6 by phosphorylating USP20 on Ser334 and reducing USP20:TRAF6 association."

No evidence text available
MTOR affects USP20
| 6 3
MTOR phosphorylates USP20. 3 / 3
| 2 1

sparser
"After insulin stimulation, USP20 is phosphorylated by the mammalian target of rapamycin complex 1 (mTORC1) and then stabilizes the HMGCR complex on the endoplasmic reticulum via interaction with Gp78 [ xref ]."

reach
"After insulin stimulation, USP20 is phosphorylated by the mammalian target of rapamycin complex 1 (mTORC1) and then stabilizes the HMGCR complex on the endoplasmic reticulum via interaction with Gp78 [50]."

reach
"In feeding state, glucose and insulin activate mammalian target of rapamycin 1 that phosphorylates the deubiquitylase ubiquitin-specific peptidase 20 (USP20)."
MTOR phosphorylates USP20 on S132. 3 / 3
| 2 1

sparser
"Lu et al. demonstrated that mammalian target of rapamycin complex 1 phosphorylates USP20 at S132 and S134 and then stabilizes HMGCR, increasing cholesterol biosynthesis in the liver [ xref ]."

reach
"Lu et al. demonstrated that mammalian target of rapamycin complex 1 phosphorylates USP20 at S132 and S134 and then stabilizes HMGCR, increasing cholesterol biosynthesis in the liver [43]."

reach
"Lu et al. demonstrated that mechanistic target of rapamycin complex 1 phosphorylated USP20 at serine 132 and serine 134 and then increased the stability of HMG-CoA reductase (HMGCR), the rate-limiting enzyme in the cholesterol biosynthetic pathway [43]."
MTOR phosphorylates USP20 on S134. 3 / 3
| 2 1

sparser
"Lu et al. demonstrated that mammalian target of rapamycin complex 1 phosphorylates USP20 at S132 and S134 and then stabilizes HMGCR, increasing cholesterol biosynthesis in the liver [ xref ]."

reach
"Lu et al. demonstrated that mammalian target of rapamycin complex 1 phosphorylates USP20 at S132 and S134 and then stabilizes HMGCR, increasing cholesterol biosynthesis in the liver [43]."

reach
"Lu et al. demonstrated that mechanistic target of rapamycin complex 1 phosphorylated USP20 at serine 132 and serine 134 and then increased the stability of HMG-CoA reductase (HMGCR), the rate-limiting enzyme in the cholesterol biosynthetic pathway [43]."
USP20 affects SNAI2
1 | 5 2
USP20 activates SNAI2.
| 3
USP20 activates SNAI2. 3 / 3
| 3

reach
"Li et al. first identified SNAI2 as one substrate of USP20, they found that USP20 can increase the stability of SNAI2, and subsequently increase the migration and invasion of ER breast cancer cell lines [67]."

reach
"In addition, USP20 can promote breast cancer metastasis by stabilizing SNAI2 [32]."

reach
"Research has shown that USP20, a deubiquitinase, promotes breast cancer metastasis by stabilizing SNAI2 [39]."
USP20 increases the amount of SNAI2.
| 2
USP20 increases the amount of SNAI2. 2 / 2
| 2

reach
"Knockdown of USP20 suppressed cell proliferation, migration, and invasion and decreased the levels of SOX4, N-cadherin, Snail, and Slug but increased the E-cadherin level[53]."

reach
"Further investigation demonstrated that USP20 knockdown inhibited the migration and invasion of breast cancer cells by modulating the expression of Slug [ 89 ]."
USP20 binds SNAI2.
| 2
| 2

sparser
"Another important regulating mechanism in breast cancer is the USP20-SNAI2 axis."

sparser
"Thus, their study suggested that USP20-SNAI2 axis may serve as a novel therapeutic target axis in breast cancer."
USP20 deubiquitinates SNAI2.
1 |
USP20 deubiquitinates SNAI2. 1 / 1
1 |

"Here, we conducted comprehensive gain- and loss-of-function screens using a human DUB cDNA library of 65 genes and an siRNA library of 98 genes, and identified <span class="match term0">USP20</span> as a deubiquitinase (DUB) that regulates <span class="match term1">SNAI2</span> ubiquitination and stability"
| 2 6
| 2 3
| 2 3

reach
"Ubiquitin-specific peptidase 20 (USP20) reduces NFkappaB activation triggered by proinflammatory stimuli, and USP20 activity attenuates atherosclerosis in mice."

eidos
"147 In addition , USP20 attenuated atherosclerosis in an LDLr - / - mouse model by inhibiting NF-kappaB activation in vivo.148 Therefore , increased expression / activity of USP20 may offer protection against inflammatory pathways in patients with ISR ."

reach
"Considering that vascular inflammation contributes to the pathogenesis of atherosclerosis, USP20 in smooth muscle cells is likely to attenuate the incidence of atherosclerosis."

eidos
"Ubiquitin-specific peptidase 20 ( USP20 ) reduces NFkappaB activation triggered by pro-inflammatory stimuli , and USP20 activity attenuates atherosclerosis in mice ."

reach
"Moreover, ectopic expression of Usp20 in smooth muscle cells significantly reduced aortic atherosclerosis in LdlrKO mice, while the introduction of the dominant negative form of USP20 increased atherosclerotic plaques [216]."
USP20 activates Atherosclerosis.
| 3

reach
"In conclusion, inhibition of Usp20 by an LNP–siRNA complex can alleviate hyperlipidemia and prevent atherosclerosis."

reach
"The results showed that silencing Usp20 by LNP-siRNA reduced lipid contents in serum, decreased body weight, and prevented atherosclerosis."

reach
"In Ldlr -/- mice, silencing Usp20 by siRNA decreased lipid levels and prevented atherosclerosis."
INS affects USP20
| 8
INS phosphorylates USP20. 4 / 4
| 4

reach
"In cancer cells, feeding-induced nutrient stress caused the accumulation of insulin and glucose, which stimulated mTORC1 to phosphorylate USP20."

reach
"They found that elevated postprandial blood glucose and insulin levels activate mTORC1 (mechanistic target of rapamycin complex 1), which stabilizes HMGCR (3-hydroxy-methylglutaryl coenzyme A reductase), phosphorylates USP20 (ubiquitin-specific peptidase 20), and upregulates cholesterol biosynthesis."

reach
"The postprandial increase in insulin and glucose activates mammalian target of rapamycin 1 to phosphorylate the deubiquitylase ubiquitin-specific peptidase 20 (USP20)."

reach
"Subsequently, the upregulation of postprandial glucose and insulin levels promotes the phosphorylation of USP20 (122)."
INS phosphorylates USP20 on S134. 2 / 2
| 2

reach
"Lu et al. [28] found that elevated insulin and glucose concentrations stimulate mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is then recruited to HMGCR to stabilize its structure and antagoni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Elevated postprandial glucose and insulin levels stimulate mTORC1 to phosphorylate S132 and S134 in USP20."
INS phosphorylates USP20 on S132. 2 / 2
| 2

reach
"Lu et al. [28] found that elevated insulin and glucose concentrations stimulate mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is then recruited to HMGCR to stabilize its structure and antagoni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Elevated postprandial glucose and insulin levels stimulate mTORC1 to phosphorylate S132 and S134 in USP20."
VAPB affects USP20
5 | 1 1
5 | 1 1

sparser
"These findings imply a potential functional interaction between USP20 and VAPB in the regulation of reticulophagy, particularly under starvation conditions."

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available

reach
"These findings imply a potential functional interaction between USP20 and VAPB in the regulation of reticulophagy, particularly under starvation conditions."
USP20 affects RIPK1
2 1 | 3 1
USP20 deubiquitinates RIPK1.
1 | 2
USP20 deubiquitinates RIPK1. 3 / 3
1 | 2

reach
"TNF evoked = ~ 2-fold more RIPK1 ubiquitination in SMC-DN-USP20-transgenic than in control SMCs, and RIPK1 was deubiquitinated by purified USP20 in vitro."

reach
"USP20 reduces atherogenic signaling in smooth muscle cells by deubiquitinating RIPK1 (receptor interacting serine/threonine kinase 1) in response to TNF (tumor necrosis factor) and IL1B (interleukin 1 beta) stimulation [51]."

"TNF evoked -2-fold more <span class="match term1">RIPK1</span> ubiquitination in SMC-DN-<span class="match term0">USP20</span>-Tg than in control SMCs, and <span class="match term1">RIPK1</span> was deubiquitinated by purified <span class="match term0">USP20</span> in vitro."
USP20 ubiquitinates RIPK1.
| 1 1
USP20 ubiquitinates RIPK1. 2 / 2
| 1 1

reach
"USP20 reduces atherogenic signaling in smooth muscle cells by deubiquitinating RIPK1 (receptor interacting serine/threonine kinase 1) in response to TNF (tumor necrosis factor) and IL1B (interleukin 1 beta) stimulation [51]."

sparser
"Antagonizing USP20 activity in SMCs by low-level overexpression of DN-USP20 augmented TNF-induced RIPK1 ubiquitination ( xref )."
USP20 binds RIPK1.
2 |
2 |

No evidence text available

No evidence text available
USP20 affects PKM2
| 4 3
USP20 binds PKM2.
| 2 3
| 2 3

reach
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability."

sparser
"Additionally, Baek et al. found that USP20 interacts with PKM2 and downregulates the ubiquitination level of PKM2 [ xref ]."

reach
"In addition, both USP20 [20] and HAUSP [44] can bind to PKM2 to regulate PKM2’s ubiquitination.The present study identified USP4 as a PKM2 interaction protein and found that USP4 can decrease the ubiquitination of PKM2."

sparser
"Apart from interacting with E3 ubiquitin ligases, PKM2 does also bind the deubiquitin enzyme USP20 (ubiquitin-specific protease, a deubiquitin enzyme)."

sparser
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability ( xref )."
USP20 decreases the amount of PKM2.
| 1
USP20 decreases the amount of PKM2. 1 / 1
| 1

reach
"Additionally, Baek et al. found that USP20 interacts with PKM2 and downregulates the ubiquitination level of PKM2 [20]."
USP20 activates PKM2.
| 1
USP20 activates PKM2. 1 / 1
| 1

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects Neoplasms
| 7
| 7

reach
"Animal studies demonstrated that USP20 depletion significantly inhibited tumor growth and increased the antitumor efficacy of OXA."

reach
"Third, the mechanism by which USP20 promotes tumor progression in this review has been elucidated in a few types of cancer cells, but the mechanism is unclear in most cancer cell lines that highly express USP20."

reach
"USP20 may promote tumor metastasis and is associated with immune infiltration and drug resistance in CRC."

reach
"USP20 can support progression of variety of tumors."

reach
"It is found that knockdown of USP20 significantly reduced tumor growth and increased the antitumor efficacy of OXA (5 mg/kg, twice a week) 20 in vivo (Figure S2D)."

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We speculate that USP20 may promote tumor progression by promoting lymph node metastasis."

reach
"However, these data are contradicted by recent findings showing that USP20 promotes proliferation, invasion, migration, and chemoresistance of multiple cancer cells via deubiquitylation of β-catenin [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"67 A recent study reported that USP20 stabilizes β‐catenin and activates Wnt/β‐catenin signaling pathway, thereby promoting the migration, invasion, and proliferation of cancer cells."

reach
"And they also observed that USP20 overexpression markedly increased cell proliferation, migration and invasion in these cancer cell lines."

reach
"The results showed that overexpression of USP20 significantly enhanced SW480 cell migration and invasion ( Figures 8D–G )."

reach
"Transwell results showed that knockdown of USP20 significantly decreased SW480 cell migration and invasion ( Figures 8K–N )."

reach
"Li et al. first identified SNAI2 as one substrate of USP20, they found that USP20 can increase the stability of SNAI2, and subsequently increase the migration and invasion of ER breast cancer cell lines [67]."

reach
"Another DUB, USP20, promoted transwell migration and invasion through the deubiquitination of SNAIL family transcriptional repressor 2 (SNAI2)."
USP20 affects IL1B
| 1 6
USP20 inhibits IL1B.
| 1 2
USP20 inhibits IL1B. 3 / 3
| 1 2

reach
"Silencing USP20 in SMCs with siRNA (small interfering RNA) augmented NFkappaB activation by = ~ 50% in response to either TNF or IL-1beta (interleukin-1beta)."

eidos
"For example , USP11 negatively regulates TNFalpha-induced NF-kappaB activation associated with IkappaBalpha and attenuates IkappaBalpha degradation [ 34 ] ; USP20 deubiquitinates TRAF6 and suppresses interleukin 1beta ( IL-1beta ) - and Tax-induced NF-kappaB activation [ 40 ] ; Katrin et al. showed that USP15 regulates IkappaBalpha / NF-kappaB by deubiquitinylation IkappaBalpha [ 44 ] ; and USP31 inhibits TNFalpha , CD40 , TRAF2 , TRAF6 and IKKbeta-mediated NF-kappaB activation [ 45 ] ."

reach
"At the same time, Usp20 silencing strongly augmented NF-κB activation in response to stimulation with TNF-α or IL-1β [216]."
USP20 activates IL1B.
| 2
USP20 activates IL1B. 2 / 2
| 2

reach
"We immunoprecipitated endogenously expressed TRAF6 from SMCs that had been transduced with equivalent levels of HA-tagged USP20-WT or HA-tagged USP20-S334A and stimulated with IL-1β."

reach
"IL-1β signaling was also found to be promoted by IRAK1 induced phosphorylation of USP20 in vascular smooth muscle cells and vascular inflammation [107,108]."
USP20 ubiquitinates IL1B.
| 1
USP20 ubiquitinates IL1B. 1 / 1
| 1

reach
"USP20 reduces atherogenic signaling in smooth muscle cells by deubiquitinating RIPK1 (receptor interacting serine/threonine kinase 1) in response to TNF (tumor necrosis factor) and IL1B (interleukin 1 beta) stimulation [51]."
USP20 deubiquitinates IL1B.
| 1
USP20 deubiquitinates IL1B. 1 / 1
| 1

reach
"USP20 reduces atherogenic signaling in smooth muscle cells by deubiquitinating RIPK1 (receptor interacting serine/threonine kinase 1) in response to TNF (tumor necrosis factor) and IL1B (interleukin 1 beta) stimulation [51]."
USP20 affects CTSL
| 7
| 7

sparser
"Co‐immunoprecipitation (co‐IP) assays demonstrated a robust interaction between endogenous USP20 and CTSL in both FaDu and HN8 cells, rather than USP14 and USP12 (Figure  xref and Figure xref )."

sparser
"These findings not only advance our understanding of EMT regulation but also underscore the therapeutic value of targeting the USP20CTSL axis in epithelial cancers."

sparser
"Next, we examined whether Diclofenac interferes with the USP20CTSL axis."

sparser
"In this study, the USP20CTSL axis was identified as a molecular marker and potential therapeutic target for metastatic HNSCC."

sparser
"Mechanistically, our data suggest that USP20 interacts with CTSL and promotes its expression by preventing STUB1‐mediated ubiquitination and degradation."

sparser
"To further assess the therapeutic potential of targeting the USP20CTSL axis, mice inoculated with tumour cells via tail vein injection were treated with the small‐molecule USP20 inhibitor G‐3A. As illustrated in the experimental scheme (Figure  xref ), compared with the control group, G‐3A significantly reduced the frequency and size of lung metastases, whereas CTSL overexpression partially restored the metastatic burden (Figure  xref )."

sparser
"In this study, the USP20CTSL axis was identified as a molecular marker and potential therapeutic target for metastatic HNSCC, providing new insights for the development of USP20‐targeted therapies."
USP20 affects CCP110
2 | 1 3
2 | 1 1

No evidence text available

sparser
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays ( xref ) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 ( xref )."

No evidence text available

reach
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
MAPK6 affects USP20
2 | 3 2
MAPK6 binds USP20.
2 | 2 2
2 | 2 2

sparser
"They demonstrated that USP20 overexpression could enhance HeLa cell migration, and they further examined the impact of the USP20-ERK3 axis in regulating the migration of breast cancer cell lines [ xref ]."

sparser
"These results indicate that the USP20-ERK3 axis can promote the migration of breast cancer cell lines, suggesting that USP20 may play an important role in the tumorigenesis of breast cancer."

reach
"USP20 interacts with and deubiquitinates ERK3 both in vitro and in intact cells."

No evidence text available

reach
"The interaction between USP20 and ERK3 was demonstrated at endogenous levels of both the proteins in cultured cells."

No evidence text available
MAPK6 activates USP20.
| 1
MAPK6 activates USP20. 1 / 1
| 1

reach
"Mechanistically, ERK3 expression level in cancer cells was shown to be upregulated by BRAF (through increasing ERK3 mRNA) 11, BMI1 (by suppressing let-7i which targets ERK3 mRNA) 12 and USP20 (by deubiquitinating and stabilizing ERK3 protein) 13."
CTSL affects USP20
| 7
| 7

sparser
"Co‐immunoprecipitation (co‐IP) assays demonstrated a robust interaction between endogenous USP20 and CTSL in both FaDu and HN8 cells, rather than USP14 and USP12 (Figure  xref and Figure xref )."

sparser
"These findings not only advance our understanding of EMT regulation but also underscore the therapeutic value of targeting the USP20CTSL axis in epithelial cancers."

sparser
"Next, we examined whether Diclofenac interferes with the USP20CTSL axis."

sparser
"In this study, the USP20CTSL axis was identified as a molecular marker and potential therapeutic target for metastatic HNSCC."

sparser
"Mechanistically, our data suggest that USP20 interacts with CTSL and promotes its expression by preventing STUB1‐mediated ubiquitination and degradation."

sparser
"To further assess the therapeutic potential of targeting the USP20CTSL axis, mice inoculated with tumour cells via tail vein injection were treated with the small‐molecule USP20 inhibitor G‐3A. As illustrated in the experimental scheme (Figure  xref ), compared with the control group, G‐3A significantly reduced the frequency and size of lung metastases, whereas CTSL overexpression partially restored the metastatic burden (Figure  xref )."

sparser
"In this study, the USP20CTSL axis was identified as a molecular marker and potential therapeutic target for metastatic HNSCC, providing new insights for the development of USP20‐targeted therapies."
CCP110 affects USP20
2 | 1 3
2 | 1 1

No evidence text available

sparser
"The interactions with USP20/33 are most likely direct because (1) bacterially expressed GST-USP33 and USP20 bind to in vitro translated CP110 in binding assays ( xref ) and (2) Neurl4 depletion does not affect the binding between USP33 and CP110 ( xref )."

No evidence text available

reach
"Since USP33 and USP20 interact with CP110 at centrosomes, and CP110 protein levels correlated with USP33 and USP20 during the cell cycle, we investigated whether USP33 or USP20 might stabilize CP110."
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."

reach
"Genetic deletion or pharmacological inhibition of USP20 significantly reduce diet-induced weight gain, decrease lipid levels in the serum and liver, improve insulin sensitivity, and increase energy ex[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Gene deletion or pharmacological inhibition of USP20 significantly reduces diet‐induced weight gain, lowers serum and hepatic lipid levels, improves insulin sensitivity, and increases energy expenditure [47]."

reach
"Genetic deletion or pharmacological inhibition of USP20 markedly decreases diet induced body weight gain, reduces lipid levels in the serum and liver, improves insulin sensitivity and increases energy expenditure."

reach
"We demonstrated that silencing of hepatic Usp20 by siRNA decreased body weight, improved insulin sensitivity, and increased energy expenditure through elevating UCP1."

reach
"USP20 links feeding induced cholesterol synthesis and energy expenditure."

reach
"Genetic deletion or pharmacological inhibition of USP20 reduces body weight, increases energy expenditure, and improves glucose clearance."

reach
"Activation of mTORC1 after feeding increases phosphorylation of ubiquitin-specific peptidase 20 (USP20) which stabilizes HMG-CoA reductase (HMGCR) and therefore promotes cholesterol synthesis."

reach
"This licenses USP20’s recruitment into the HMGCR complex and antagonizes its degradation, thereby stabilizing HMGCR and promoting cholesterol synthesis [133]."

reach
"USP20 stabilizes 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) to increase liver cholesterol biosynthesis, which aggravates metabolic disorders [14]."

reach
"In sum, these findings suggest that TBBPA enhances cholesterol synthesis by increasing USP20 protein levels, which in turn stabilizes the HMGCR protein."

reach
"USP20 was recruited to the HMGCR complex and antagonized its degradation, up-regulating cholesterol synthesis and converting absorbed glucose and other nutrients into cholesterol."

reach
"They found that elevated postprandial blood glucose and insulin levels activate mTORC1 (mechanistic target of rapamycin complex 1), which stabilizes HMGCR (3-hydroxy-methylglutaryl coenzyme A reductase), phosphorylates USP20 (ubiquitin-specific peptidase 20), and upregulates cholesterol biosynthesis."
USP20 affects Tax
| 6
USP20 deubiquitinates Tax.
| 4
USP20 deubiquitinates Tax. 4 / 4
| 4

reach
"For example, Junichiro et al. demonstrated that USP21 constitutively deubiquitinates RIP1 in vitro and in vivo [XREF_BIBR]; and a previous study has reported that USP20 deubiquitinates TRAF6 and Tax in vivo [XREF_BIBR]."

reach
"We find that USP20 deubiquitinates Tax and inhibits Tax induced NF-kappaB activation, consistent with Tax being a substrate of USP20."
| PMC

reach
"Here, we report that ubiquitin specific peptidase USP20 deubiquitinates TRAF6 and Tax and suppresses interleukin 1beta (IL-1beta) - and Tax induced NF-kappaB activation."

reach
"In further support of this, the deubiquitinating enzyme (DUB) USP20 deubiquitinates Tax to negatively regulate activation of IKK [XREF_BIBR]."
USP20 activates Tax.
| 2
USP20 activates Tax. 2 / 2
| 2

reach
"USP20, by promoting the stability of TRAF6 and Tax, contributes to the activation of NF-κB, including its activation induced by IL-1β."

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects ATR
1 | 2 2
USP20 binds ATR.
1 | 1 2
1 | 1 2

sparser
"We further identified the replication stress signal, ATR, which interacts with USP20."

sparser
"We conducted Co‐IP analysis in Huh‐7 cell lines and observed the interaction between ATR and USP20 (Figure  xref )."

No evidence text available

reach
"We conducted Co‐IP analysis in Huh‐7 cell lines and observed the interaction between ATR and USP20 (Figure 6A)."
USP20 activates ATR.
| 1
USP20 activates ATR. 1 / 2
| 1

reach
"59 , 60 , 61 , 62 , 63 , 64 USP20 mediates ATR‐mediated DNA damage response through the stabilization of Claspin."
RAD17 affects USP20
4 | 2
4 | 2

No evidence text available

No evidence text available

No evidence text available

sparser
"We demonstrate that USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure."

sparser
"ATM/ATR-mediated phosphorylation of USP20 does not affect its DUB activity; rather, this USP20 phosphorylation enhances the association of USP20 with Rad17 and thereby enhances USP20-mediated deubiquitination of Rad17 ( xref )."

No evidence text available
Luteolin affects USP20
| 5
Luteolin phosphorylates USP20 on S134. 2 / 2
| 2

reach
"Lu et al. demonstrated that mammalian target of rapamycin complex 1 phosphorylates USP20 at S132 and S134 and then stabilizes HMGCR, increasing cholesterol biosynthesis in the liver [43]."

reach
"Lu et al. demonstrated that mechanistic target of rapamycin complex 1 phosphorylated USP20 at serine 132 and serine 134 and then increased the stability of HMG-CoA reductase (HMGCR), the rate-limiting enzyme in the cholesterol biosynthetic pathway [43]."
Luteolin phosphorylates USP20 on S132. 2 / 2
| 2

reach
"Lu et al. demonstrated that mammalian target of rapamycin complex 1 phosphorylates USP20 at S132 and S134 and then stabilizes HMGCR, increasing cholesterol biosynthesis in the liver [43]."

reach
"Lu et al. demonstrated that mechanistic target of rapamycin complex 1 phosphorylated USP20 at serine 132 and serine 134 and then increased the stability of HMG-CoA reductase (HMGCR), the rate-limiting enzyme in the cholesterol biosynthetic pathway [43]."
Luteolin phosphorylates USP20. 1 / 1
| 1

reach
"Recently, Lu et al. reported that activation of mTORC1 could phosphorylate USP20 to antagonist HMGCR degradation [44]."
VAPA affects USP20
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
5 |
USP20 is inactive.
4 |
Ubiquitinated USP20 is inactive. 3 / 3
3 |

"HERC2 promotes USP20 degradation.|Under unperturbed condition, HERC2 ubiquitinates USP20 and promotes ubiquitination mediated proteasomal degradation of USP20, regulating the status of K48 linked polyubiquitination of CLASPIN and ensuring appropriate protein levels of CLASPIN during the S-phase."

"USP20 is regulated by HERC2 following DNA damage or replication stress.The HECT domain of WT HERC but not the catalytic inactive mutant (CA) ubiquitinated USP20 in vitro (Figure ​(Figure4G).4G). Taken together, these results suggested that HERC2 functions as an E3 ligase of USP20 and negatively regulates USP20 in unstressed cells."

"VDU1 and VDU2 could be important substrates of pVHL E3 ligase complex. Finally, we demonstrate that VDU2 can also be ubiquitinated and degraded in a pVHL-dependent manner.pVHL mediates the degradation of hVDU2 by proteasome. we have demonstrated that both VDU1 and VDU2 also bind to the β-domain of pVHL and several naturally occurring mutations in the β-domain disrupt the interaction between VDU1/VDU2 and pVHL. If pVHL is mutated either in the α-domain or β-domain, VDU1 and VDU2 would not be ubiquitinated and degraded properly. This could lead to accumulation of these two proteins in the cells. Together, our results suggest that VDU1 and VDU2 might be relevent to pVHL-related tumorigenesis."
Phosphorylated USP20 is inactive. 1 / 1
1 |

"On the other hand, USP20 is phosphorylated by ATR, which disrupts the interaction between USP20 and HERC2, resulting in USP20 stabilization.|USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation."
USP20 is active.
1 |
Phosphorylated USP20 is active. 1 / 1
1 |

"USP20 phosphorylation by ATR is important for its stabilization and checkpoint activation"
USP20 affects VAPA
5 |
5 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP20 affects TNF
| 4 1
USP20 inhibits TNF.
| 4
USP20 inhibits TNF. 4 / 4
| 4

reach
"Silencing USP20 in SMCs with siRNA (small interfering RNA) augmented NFkappaB activation by = ~ 50% in response to either TNF or IL-1beta (interleukin-1beta)."

reach
"USP20 (Ubiquitin Specific Protease 20) Inhibits TNF (Tumor Necrosis Factor)-Triggered Smooth Muscle Cell Inflammation and Attenuates Atherosclerosis."

reach
"Conclusions- USP20 attenuates TNF- and IL-1beta-evoked atherogenic signaling in SMCs, by deubiquitinating RIPK1, among other signaling intermediates."

reach
"XREF_BIBR USP20 inhibits NF-kappaB activation via Toll like receptor (TLR) 4, XREF_BIBR IL-1R, and the TNF XREF_BIBR inflammatory pathway in VSMCs."
USP20 binds TNF.
| 1
| 1

sparser
"Co-immunoprecipitation experiments revealed that USP20 associates with several components of the TNF receptor-1 signaling pathway including receptor-interacting protein kinase 1 (RIPK1), a critical checkpoint in TNF-induced NFκB activation and inflammation."
USP20 affects STAT3
| 5
| 5

sparser
"We next performed validation of the mass spectrometry by immunoprecipitation and found that USP20 interacts with STAT3 in heart tissues and neonatal rat cardiomyocytes (NRCMs) following Ang II treatment (Figure  xref ), suggesting that USP20 directly binds to the STAT3 protein in cardiomyocytes."

sparser
"By co‐transfecting STAT3 and the mutated USP20 plasmids into NIH3T3 cells, we found that USP20 could not bind to STAT3 when amino acids 791 to 894 were deleted, whereas mutations in other domains did not affect such binding (Figure  xref )."

sparser
"In this research, we identified an unannotated cardiomyocyte‐specific USP20STAT3 axis, highlighting its role in regulation of cardiac hypertrophy ( Structured Graphical Abstract )."

sparser
"We hypothesized that USP20STAT3 axis ameliorates cardiac hypertrophy through transcriptional regulation of Carm1 ."

sparser
"We observed that the K177R mutation did not disrupt USP20STAT3 binding but resulted in significantly lower STAT3 ubiquitination compared to the wild‐type, and this reduction was not further decreased by USP20 overexpression (Figure  xref )."
USP20 affects SOX4
1 | 1 2 1
USP20 binds SOX4.
1 | 1 1
1 | 1 1

sparser
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."

No evidence text available

reach
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."
USP20 increases the amount of SOX4.
| 1
USP20 increases the amount of SOX4. 1 / 1
| 1

reach
"USP20 was found to modulate SOX4 protein expression."
USP20 activates SOX4.
| 1
USP20 activates SOX4. 1 / 1
| 1

eidos
"Finally , through rescue assays , we revealed that USP20 mediated SOX4 expression to accelerate CRC progression ."
USP20 affects PTEN
| 5
| 5

sparser
"Together, these results suggested that USP20 could interact with PTEN to regulate neuroinflammation and neuronal death in microglia and cortical neurons, respectively."

sparser
"Among the numerous USP members, USP20 interacts with PTEN and affects neuroinflammation via Toll-like receptor signaling pathway (Pan et al. xref ; Jean-Charles et al. xref )."

sparser
"Here, in our study, we found that USP20 over-expression promoted PTEN expression, and importantly USP20 could directly bind to PTEN."

sparser
"Notably, mechanistic analysis indicated that USP20 directly interacted with PTEN, thereafter reducing inflammatory response in microglial cells and mitigating neuronal death in cortical neurons upon O[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"These findings indicated that USP20 interacted with PTEN in cells."
USP20 affects NEURL4
3 | 2
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
USP20 affects HIF1
| 5
USP20 deubiquitinates HIF1.
| 2
USP20 deubiquitinates HIF1. 2 / 2
| 2

reach
"VDU2 can interact with HIF-1α, a tumor suppressor, to deubiquitinate and stabilize HIF-1α (Li et al., 2005)."

reach
"VDU2 (pVHL-interacting deubiquitinating enzyme 2) interacts with and deubiquitinates HIF-1α and is itself ubiquitinated in a VHL-E3 ligase-dependent (but oxygen-independent) manner [101,102] ."
USP20 activates HIF1.
| 2
USP20 activates HIF1. 2 / 2
| 2

reach
"The deubiquitinating enzymes USP20, USP8 and UCHL1 can modulate the HIF-1α level and stability, and regulate VEGF expression (32)."

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 ubiquitinates HIF1.
| 1
USP20 ubiquitinates HIF1. 1 / 1
| 1

reach
"We identified the ubiquitin specific peptidase 20 (Usp20), which de‐ubiquitinates hypoxia‐inducible factor 1ɑ (HIF1ɑ) and prevents it from proteasomal degradation, as Foxp1 direct target gene."
| 5
| 5

reach
"Knockdown of USP20 and p62 in HeLa cells decreases cell viability and number while increasing the expression of cleaved caspase-8, caspase-3 and PARP (153)."

reach
"We observed that USP20 depletion reduced the cell viability upon the treatment of erastin, which could be rescued by Ferr‐1, suggesting that the mechanism is specific in cell ferroptosis (Figures 2F, G and S2G)."

reach
"Furthermore, reconstitution of WT USP20 but not USP20-4 mut rescued the cell viability after UV treatment and restored UV or HU induced intra-S-phase checkpoint."

reach
"Consistent with the USP20‐knockdown results, GSK2643943A decreased the cell viability and increased erastin‐induced viability inhibition and increased ferrous iron levels and lipid ROS levels, while decreased the GSH levels in Huh‐7 cells (Figures 3A–G)."

reach
"USP20 depletion also reduced the cell viability upon the treatment of erastin, which could be recovered by Ferr‐1, suggesting that the mechanism is specific in cell ferroptosis."
STAT3 affects USP20
| 5
| 5

sparser
"We next performed validation of the mass spectrometry by immunoprecipitation and found that USP20 interacts with STAT3 in heart tissues and neonatal rat cardiomyocytes (NRCMs) following Ang II treatment (Figure  xref ), suggesting that USP20 directly binds to the STAT3 protein in cardiomyocytes."

sparser
"By co‐transfecting STAT3 and the mutated USP20 plasmids into NIH3T3 cells, we found that USP20 could not bind to STAT3 when amino acids 791 to 894 were deleted, whereas mutations in other domains did not affect such binding (Figure  xref )."

sparser
"In this research, we identified an unannotated cardiomyocyte‐specific USP20STAT3 axis, highlighting its role in regulation of cardiac hypertrophy ( Structured Graphical Abstract )."

sparser
"We hypothesized that USP20STAT3 axis ameliorates cardiac hypertrophy through transcriptional regulation of Carm1 ."

sparser
"We observed that the K177R mutation did not disrupt USP20STAT3 binding but resulted in significantly lower STAT3 ubiquitination compared to the wild‐type, and this reduction was not further decreased by USP20 overexpression (Figure  xref )."
PTEN affects USP20
| 5
| 5

sparser
"Together, these results suggested that USP20 could interact with PTEN to regulate neuroinflammation and neuronal death in microglia and cortical neurons, respectively."

sparser
"Among the numerous USP members, USP20 interacts with PTEN and affects neuroinflammation via Toll-like receptor signaling pathway (Pan et al. xref ; Jean-Charles et al. xref )."

sparser
"Here, in our study, we found that USP20 over-expression promoted PTEN expression, and importantly USP20 could directly bind to PTEN."

sparser
"Notably, mechanistic analysis indicated that USP20 directly interacted with PTEN, thereafter reducing inflammatory response in microglial cells and mitigating neuronal death in cortical neurons upon O[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"These findings indicated that USP20 interacted with PTEN in cells."
PKM2 affects USP20
| 2 3
| 2 3

reach
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability."

sparser
"Additionally, Baek et al. found that USP20 interacts with PKM2 and downregulates the ubiquitination level of PKM2 [ xref ]."

reach
"In addition, both USP20 [20] and HAUSP [44] can bind to PKM2 to regulate PKM2’s ubiquitination.The present study identified USP4 as a PKM2 interaction protein and found that USP4 can decrease the ubiquitination of PKM2."

sparser
"Apart from interacting with E3 ubiquitin ligases, PKM2 does also bind the deubiquitin enzyme USP20 (ubiquitin-specific protease, a deubiquitin enzyme)."

sparser
"USP20 interaction with PKM2 was suggested to apparently enhance its protein stability ( xref )."
NEURL4 affects USP20
3 | 2
3 |

No evidence text available

No evidence text available

No evidence text available
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
MTORC1 affects USP20
| 3 1
MTORC1 phosphorylates USP20. 4 / 4
| 3 1

sparser
"USP20 stabilizes HMG-CoA reductase (HMGCR), the rate-limiting enzyme in the cholesterol biosynthetic pathway, and USP20 was phosphorylated by mTORC1 at S132 and S134 xref ."

reach
"It has been reported that mTORC1 promotes cholesterol synthesis by phosphorylating USP20 to protect the HMGCR complex from degradation [ 30 ] and trigger the production of mature SREBP2 [ 33 ] to posi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"After insulin stimulation, USP20 is phosphorylated by the mammalian target of rapamycin complex 1 (mTORC1) and then stabilizes the HMGCR complex on the endoplasmic reticulum via interaction with Gp78 [50]."

reach
"They found that elevated postprandial blood glucose and insulin levels activate mTORC1 (mechanistic target of rapamycin complex 1), which stabilizes HMGCR (3-hydroxy-methylglutaryl coenzyme A reductase), phosphorylates USP20 (ubiquitin-specific peptidase 20), and upregulates cholesterol biosynthesis."
GroEL2 affects USP33
| 4
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
Glucose affects USP20
| 4
Glucose phosphorylates USP20. 2 / 2
| 2

reach
"In cancer cells, feeding-induced nutrient stress caused the accumulation of insulin and glucose, which stimulated mTORC1 to phosphorylate USP20."

reach
"The postprandial increase in insulin and glucose activates mammalian target of rapamycin 1 to phosphorylate the deubiquitylase ubiquitin-specific peptidase 20 (USP20)."
Glucose phosphorylates USP20 on S132. 1 / 1
| 1

reach
"Lu et al. [28] found that elevated insulin and glucose concentrations stimulate mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is then recruited to HMGCR to stabilize its structure and antagoni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
Glucose phosphorylates USP20 on S134. 1 / 1
| 1

reach
"Lu et al. [28] found that elevated insulin and glucose concentrations stimulate mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is then recruited to HMGCR to stabilize its structure and antagoni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP33 affects groEL2
| 4
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
| 4
| 4

reach
"The results demonstrated that in USP20 knockdown cells, the expression of wild-type USP20 or RETREG1 fully restored reticulophagy function, whereas expression of the catalytically inactive mutant USP20 or the FFAT1/2-deleted mutant USP20[ΔFFAT1/2] failed to initiate reticulophagy (Figure 7A-B)."

reach
"These observations strongly support the notion that USP20 positively regulates reticulophagy."

reach
"Additionally, USP20’s interaction with VAPs further enhances its role in promoting efficient reticulophagy."

reach
"USP20 deubiquitinates and stabilizes the reticulophagy receptor RETREG1/FAM134B to drive reticulophagy."
| 4

reach
"They demonstrated that USP20 overexpression could enhance HeLa cell migration, and they further examined the impact of the USP20-ERK3 axis in regulating the migration of breast cancer cell lines [58]."

reach
"Transwell results showed that knockdown of USP20 significantly decreased SW480 cell migration and invasion ( Figures 8K–N )."

reach
"The results showed that overexpression of USP20 significantly enhanced SW480 cell migration and invasion ( Figures 8D–G )."

reach
"In addition, a novel role for USP20 in promoting actin cytoskeleton organization and cell migration was revealed and linked to its regulation of ERK3 expression."
USP20 affects USP33, and groEL2
| 4
| 4

sparser
"Similar to our previous demonstration with hSP56 and VDU1 interactions (14), VDU2 (red fluorescence from anti-HA/anti-mouse IgG-Texas Red) and hSP56 (green fluorescence from hSP56-EGFP) overlapped in the cytoplasm ( xref D)."

sparser
"We investigated the interactions of hSP56 with VDU1 and VDU2 further by co-immunoprecipitation."

sparser
"HSP56 interacts with both VDU1 and VDU2."

sparser
"HSP56 was co-immunoprecipitated specifically with either VDU1-HA or VDU2-HA using anti-HA antibody, but not using normal mouse IgG ( xref C), confirming that hSP56 interacts with both VDU1 and VDU2."
USP20 affects DUSP2
| 4
| 4

sparser
"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We were successful in producing the USP4-DUSP, USP11-DUSP, USP15-DUSP, USP20-DUSP2 and USP33-DUSP2 recombinant proteins in Escherichia coli and purified them to near homogeneity from the soluble fract[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"For USP15-DUSP and USP33-DUSP2, the best UbVs exhibited affinities in the low nanomolar range (K D ∼ 10 nM), whereas for USP11-DUSP and USP20-DUSP2, affinities were in the mid-nanomolar range (K D ∼ 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We only isolated one UbV binding to USP20-DUSP2 (20D2.1), but fortunately, it proved to be absolutely specific for its cognate protein."
| 3

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Previous publications have identified β-catenin as an oncogene [62], prompting us to speculate that USP20 promotes tumorigenesis by regulating β-catenin stability."

reach
"USP20 promotes the tumorigenesis of several cancer types, such as breast and cervical cancer; however, it exhibits inhibitory effects on GC [16]."
USP20 affects CS
| 4
CS binds USP20. 4 / 4
| 4

sparser
"As expected, the wild-type USP20 significantly decreased the polyubiquitination of PINK1, whereas the USP20-CS mutant did not (Fig.  xref A and B)."

sparser
"HEK293 cells were transfected with a plasmid encoding the catalytically inactive USP20-CS mutant and/or treated with CCCP."

sparser
"To confirm this, HEK293 cells were transfected for 24 h with a plasmid encoding PINK1-Myc, either alone or together with a plasmid encoding USP20-WT or its catalytically inactive USP20-CS mutant, and an immunoprecipitation assay was performed (Fig.  xref A)."

sparser
"Immunoblot analysis of the lysates showed that the LC3-II level increased under CCCP treatment and slightly decreased with the overexpression of the USP20-CS mutant (Fig.  xref A)."
USP20 affects CBX7
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
USP20 affects Body Weight
| 4
| 4

reach
"We demonstrated that silencing of hepatic Usp20 by siRNA decreased body weight, improved insulin sensitivity, and increased energy expenditure through elevating UCP1."

reach
"The results showed that silencing Usp20 by LNP-siRNA reduced lipid contents in serum, decreased body weight, and prevented atherosclerosis."

reach
"Liver specific genetic deletion of USP20 or its pharmacological inhibition decreases diet-induced body weight gain and changes a number of other metabolic parameters accordingly [69] ."

reach
"Genetic deletion or pharmacological inhibition of USP20 reduces body weight, increases energy expenditure, and improves glucose clearance."
USP20 affects ARRB2
1 | 2 1
USP20 deubiquitinates ARRB2.
| 2
USP20 deubiquitinates ARRB2. 2 / 2
| 2

reach
"54 Ubiquitinated beta-arrestin2 promotes NF-kappaB signaling in SMCs, whereas deubiquitinated beta-arrestin2 attenuates NF-kappaB signaling by scaffolding the deubiquitinase USP20, that in turn deubiquitinates TRAF6, which blocks the NF-kappaB signaling cascade."

reach
"Importantly, a double knockdown of USP20 and USP33 enhances the extent of beta-arrestin-2 ubiquitination and increases beta 2 AR degradation."
USP20 binds ARRB2.
1 | 1
1 | 1

No evidence text available

sparser
"The association of USP20 with β-arrestin2 was augmented when β-arrestin2 ubiquitination was prevented and reduced when β-arrestin2 ubiquitination was rendered constitutive."
USP20 affects APBA2
4 |
4 |

No evidence text available

No evidence text available

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No evidence text available
USP20 affects AMFR
| 1 3
| 1 3

reach
"USP20 then binds gp78 and antagonizes the degradation of HMGCR."

sparser
"One such example, USP20 interacts with gp78 and reduced the ubiquitination of HMGCR upon feeding-induced activation [ xref ]."

sparser
"When it is phosphorylated on Ser132/Ser134 by mTORC1, USP20 associates with the ubiquitin E3 ligase autocrine motility factor receptor (GP78)."

sparser
"USP20 then binds gp78 and antagonizes the degradation of HMGCR."

reach
"Animal studies demonstrated that USP20 depletion significantly inhibited tumor growth and increased the antitumor efficacy of OXA."

reach
"USP20 contributes OXA resistance and suppress ferroptosis in HCC."

reach
"Pharmacological inhibition or knockdown of USP20 in HCC cells triggered ferroptosis and reversed OXA resistance both in vitro and in vivo."

reach
"It is found that knockdown of USP20 significantly reduced tumor growth and increased the antitumor efficacy of OXA (5 mg/kg, twice a week) 20 in vivo (Figure S2D)."
USP18 affects STING1
| 4
| 4

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."
TINCR affects USP20
| 4
TINCR activates USP20. 4 / 4
| 4

reach
"Specifically, TINCR acts as a molecular sponge for miR-199a-5p and upregulates the USP20 stability through a ceRNA regulatory mechanism, promoting the upregulation of PD-L1 protein by inhibiting its ubiquitination."

reach
"Then we extended the processing time and confirmed that IFN-γ Stimulation can indeed downregulate the expression of miR-199a-5p.This is the first study to clarify the mechanism by which TINCR upregulates USP20 and PD-L1 through the dual role of ceRNA interaction and miR-199a-5p transcription inhibition."

reach
"In the cytoplasm, TINCR sponges miR-199a-5p to increase USP20 mRNA stability."

reach
"Furthermore, in the cytoplasm, TINCR potentially acts as a molecular sponge of miR-199a-5p and upregulates the stability of USP20 mRNA through a competing endogenous RNA (ceRNA) regulatory mechanism, thus promoting PD-L1 expression by decreasing its ubiquitination level."
STING1 affects USP18, and USP20
| 4
| 4

sparser
"We found that USP18 and USP20 both interact with STING in SCC9 cells."

sparser
"Immunoprecipitation revealed that both USP18 and USP20 interacted with the N-terminal region of the STING protein."

sparser
"Collectively, these results showed that USP18 and USP20 interact with STING and that a deficiency of either USP18 or USP20 affects the stability of STING in SCC9 cells."

sparser
"Our above results demonstrated that (1) USP18 and USP20 correlate with limited oHSV-1 T1012G virus yields in SCC9 cells; (2) USP18 and USP20 interact with STING and alter the levels of STING proteins in SCC9 cells; and (3) altered STING accumulation regulates viral replication in SCC9 and SCC25 cells, while we need to further examine whether the DUB activity of USP18 or USP20 is crucial for the regulation of STING stabilization and oHSV-1 T1012G virus replication."
HMGCR affects USP20
| 1 3
| 1 3

reach
"HMGCR recruited phosphorylated USP20 to form the HMGCR-USP20 complex, which protected HMGCR from proteasome-mediated degradation [177]."

sparser
"HMGCR recruited phosphorylated USP20 to form the HMGCR-USP20 complex, which protected HMGCR from proteasome-mediated degradation [ xref ]."

sparser
"By doing so, mTORC1 enhanced the USP20-HMGCR interaction and prevented HMGCR degradation."

sparser
"Furthermore, Lu XY et al. reported that mTORC1 might control cholesterol synthesis by regulating the USP20-HMGCR axis [53] , emphasizing the importance of mTORC1 activation."
DUSP2 affects USP20
| 4
| 4

sparser
"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We were successful in producing the USP4-DUSP, USP11-DUSP, USP15-DUSP, USP20-DUSP2 and USP33-DUSP2 recombinant proteins in Escherichia coli and purified them to near homogeneity from the soluble fract[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"For USP15-DUSP and USP33-DUSP2, the best UbVs exhibited affinities in the low nanomolar range (K D ∼ 10 nM), whereas for USP11-DUSP and USP20-DUSP2, affinities were in the mid-nanomolar range (K D ∼ 1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We only isolated one UbV binding to USP20-DUSP2 (20D2.1), but fortunately, it proved to be absolutely specific for its cognate protein."
CTNNB1 affects USP20
1 | 3
1 | 3

sparser
"Several ubiquitin-specific proteases (USPs), including USP2a [ xref ], USP39 [ xref ], USP47 [ xref ], and USP20 [ xref ] also directly bind to and deubiquitinate β-catenin in the cytoplasm, leading to elevated expression of Wnt target genes."

sparser
"To further investigate the role of the USP20-β-catenin axis in cancers, they detected the protein and mRNA levels of USP20 and β-catenin in colon cancer and other cancer cell lines, including osteosarcoma, cervical, breast, ovarian, and colorectal cancer cell lines [ xref ]."

No evidence text available

sparser
"Thus, the USP20-β-catenin axis may be an emerging therapeutic target in colon cancer."
CS affects USP20
| 4
CS binds USP20. 4 / 4
| 4

sparser
"As expected, the wild-type USP20 significantly decreased the polyubiquitination of PINK1, whereas the USP20-CS mutant did not (Fig.  xref A and B)."

sparser
"HEK293 cells were transfected with a plasmid encoding the catalytically inactive USP20-CS mutant and/or treated with CCCP."

sparser
"To confirm this, HEK293 cells were transfected for 24 h with a plasmid encoding PINK1-Myc, either alone or together with a plasmid encoding USP20-WT or its catalytically inactive USP20-CS mutant, and an immunoprecipitation assay was performed (Fig.  xref A)."

sparser
"Immunoblot analysis of the lysates showed that the LC3-II level increased under CCCP treatment and slightly decreased with the overexpression of the USP20-CS mutant (Fig.  xref A)."
CBX7 affects USP20
4 |
4 |

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No evidence text available

No evidence text available
APBA2 affects USP20
4 |
4 |

No evidence text available

No evidence text available

No evidence text available

No evidence text available
AMFR affects USP20
| 1 3
| 1 3

reach
"USP20 then binds gp78 and antagonizes the degradation of HMGCR."

sparser
"One such example, USP20 interacts with gp78 and reduced the ubiquitination of HMGCR upon feeding-induced activation [ xref ]."

sparser
"When it is phosphorylated on Ser132/Ser134 by mTORC1, USP20 associates with the ubiquitin E3 ligase autocrine motility factor receptor (GP78)."

sparser
"USP20 then binds gp78 and antagonizes the degradation of HMGCR."
Rep affects USP20
3 |
3 |

No evidence text available
| DOI

No evidence text available
| DOI

No evidence text available
| DOI
3 |
Pirinixic acid increases the amount of USP20. 3 / 3
3 |

No evidence text available

No evidence text available

No evidence text available
VSX2 affects USP20
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
VAP affects USP20
| 3
VAP binds USP20. 3 / 3
| 3

sparser
"To gain insight into this mechanism, we investigated the interaction between USP20 and VAPs."

sparser
"The interaction of USP20 with VAPs facilitates VAPs localization to specific ER subdomains under conditions of nutrient deprivation, where USP20 forms puncta with the reticulophagy receptor RETREG1."

sparser
"Additionally, USP20’s interaction with VAPs further enhances its role in promoting efficient reticulophagy."
USP20 affects β-arrestin2
| 3
USP20 deubiquitinates β-arrestin2.
| 2
USP20 deubiquitinates β-arrestin2. 2 / 2
| 2

reach
"For example, USP20 deubiquitinates not only TRAF6 and β-arrestin2 to reduce TLR4-induced NFκB activation (3) but also RIPK1 to prevent TNFR-induced NFκB activation (4)."

reach
"USP20 deubiquitinates TRAF6 and β-arrestin2, and the overexpression of β-arrestin2 is more effective in linking USP20 to TRAF6."
USP20 activates β-arrestin2.
| 1
USP20 activates β-arrestin2. 1 / 1
| 1

reach
"Furthermore, changes in USP20 expression could mediate inflammatory responses partly through defining the ubiquitination status of β-arrestin2 [9] ."
USP20 affects rep
3 |
3 |

No evidence text available
| DOI

No evidence text available
| DOI

No evidence text available
| DOI
| 3

reach
"An apoptosis assay showed that USP20 silencing increased the apoptosis rate of T24 and UMUC-3 cells (Fig. 3B)."

reach
"USP20 knockdown was suggested to promote apoptosis in cells [8,27] ."

reach
"FACS analysis of PI/annexin V staining showed that USP20 knockdown increased apoptosis in BC cells, but this was reversed by YAP1 overexpression (Fig. 5B)."
USP20 affects VSX2
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
USP20 affects VAP
| 3
VAP binds USP20. 3 / 3
| 3

sparser
"To gain insight into this mechanism, we investigated the interaction between USP20 and VAPs."

sparser
"The interaction of USP20 with VAPs facilitates VAPs localization to specific ER subdomains under conditions of nutrient deprivation, where USP20 forms puncta with the reticulophagy receptor RETREG1."

sparser
"Additionally, USP20’s interaction with VAPs further enhances its role in promoting efficient reticulophagy."
USP20 affects UCP1
| 3
USP20 inhibits UCP1.
| 1
USP20 inhibits UCP1. 1 / 1
| 1

reach
"These data suggest that silencing of hepatic Usp20 increases heat production through succinate-induced elevation of UCP1 in BAT and iWAT."
USP20 increases the amount of UCP1.
| 1
USP20 increases the amount of UCP1. 1 / 1
| 1

reach
"Ablation of hepatic Usp20 increases energy expenditure and UCP1 expression."
USP20 activates UCP1.
| 1
USP20 activates UCP1. 1 / 1
| 1

reach
"We demonstrated that silencing of hepatic Usp20 by siRNA decreased body weight, improved insulin sensitivity, and increased energy expenditure through elevating UCP1."
USP20 affects IRF3
| 3
USP20 inhibits IRF3.
| 2
USP20 inhibits IRF3. 2 / 2
| 2

reach
"USP18 deficiency or knockdown of USP20 resulted in enhanced K48 linked ubiquitination and accelerated degradation of STING, and impaired activation of IRF3 and NF-kappaB as well as induction of downstream genes after infection with DNA virus HSV-1 or transfection of various DNA ligands."

reach
"XREF_BIBR This study showed that USP18 or USP20 deficiency significantly inhibited HSV-I or cytosolic DNA activation of both NF-kappaB and IRF3, and type-I IFN and proinflammatory cytokine expression."
USP20 phosphorylates IRF3.
| 1
USP20 leads to the phosphorylation of IRF3. 1 / 1
| 1

reach
"Deletion of USP20 accelerates HSV-1-induced degradation of MITA and impairs phosphorylation of IRF3 and IkappaBalpha as well as subsequent induction of type I IFNs and proinflammatory cytokines after HSV-1 infection or cytoplasmic DNA challenge."
USP20 affects FOXP3
| 2 1
USP20 binds FOXP3.
| 1 1
| 1

sparser
"We found that USP20 can bind and deubiquitinate FOXP3 (Fig.  xref )."
| 1

reach
"We found that USP20 can bind and deubiquitinate FOXP3 (Fig. 4d, e)."
USP20 deubiquitinates FOXP3.
| 1
USP20 deubiquitinates FOXP3. 1 / 1
| 1

reach
"We observed that both USP11 and USP20 can act as DUBs to deubiquitinate FOXP3 (Fig. 4a)."
USP20 affects DBNL
3 |
3 |

No evidence text available

No evidence text available

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SOX4 affects USP20
1 | 1 1
1 | 1 1

sparser
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."

No evidence text available

reach
"The interaction between USP20 and SOX4 was notarized through Co-IP assay."
SLC7A11 affects USP20
| 1 2
| 1 2

reach
"Results of Co‐IP revealed the association between SLC7A11 and USP20."

sparser
"First, USP20 and SLC7A11 binds with each other."

sparser
"Recently, it was reported that USP20 directly interacts with xCT and cleaves K48‐linked polyubiquitinated xCT at K30 and K37. [ xref ] TRIM3, an E3 ubiquitin ligase, promoted xCT proteasome‐dependent degradation by catalyzing K11‐linked ubiquitination of xCT at K37. [ xref ] Notably, no reports have identified any E3 ligase‐mediated ubiquitination of the xCT protein at K4 or K12, leaving an open question regarding the identification of another antagonistic regulator responsible for ferroptosis sensitivity and ubiquitination modification of xCT at K4 or K12 in BLCA in future studies."
PRKACA affects USP20
1 2 |
PRKACA phosphorylates USP20.
1 1 |
PRKACA phosphorylates USP20 on S333. 2 / 2
1 1 |

No evidence text available

"Upon β2AR activation, a specific isoform of the second messenger cAMP-dependent protein kinase A (PKAα) rapidly phosphorylates USP20 on serine 333 located in its unique insertion domain. This phosphorylation of USP20 correlates with a characteristic SDS-PAGE mobility shift of the protein, blocks its deubiquitinase activity, promotes its dissociation from the activated β2AR complex, and facilitates trafficking of the ubiquitinated β2AR to autophagosomes, which fuse with lysosomes to form autolysosomes where receptors are degraded."
PRKACA decreases the amount of USP20.
1 |
PRKACA decreases the amount of USP20. 1 / 1
1 |

"Upon β2AR activation, a specific isoform of the second messenger cAMP-dependent protein kinase A (PKAα) rapidly phosphorylates USP20 on serine 333 located in its unique insertion domain. This phosphorylation of USP20 correlates with a characteristic SDS-PAGE mobility shift of the protein, blocks its deubiquitinase activity, promotes its dissociation from the activated β2AR complex, and facilitates trafficking of the ubiquitinated β2AR to autophagosomes, which fuse with lysosomes to form autolysosomes where receptors are degraded."
OGD/R affects USP20
| 3
OGD/R ubiquitinates USP20.
| 1
OGD/R leads to the ubiquitination of USP20. 1 / 1
| 1

reach
"We then found that OGD/R stimulation led to hyperubiquitination of USP20 in the cultured microglia and neurons ( Fig. 4 G)."
OGD/R increases the amount of USP20.
| 1
OGD/R increases the amount of USP20. 1 / 1
| 1

reach
"The in vitro experiments indicated that OGD/R treatment remarkably increased USP20 expression following reoxygenation within 3 h."
OGD/R decreases the amount of USP20.
| 1
OGD/R decreases the amount of USP20. 1 / 1
| 1

reach
"As expected, OGD/R stimulation markedly decreased USP20 expression in microglial cells and neurons, which were, however, considerably restored by USP20 over-expression ( Fig. 4 C-F)."
MCL1 affects USP20
| 1 2
| 1 2

reach
"As shown in Fig. 3 a and b, the coimmunoprecipitation assay revealed a significant endogenous binding between USP20 and MCL1."

sparser
"To further probe the mechanism by which USP20 regulates MCL1, we examined whether USP20 interacted with MCL1."

sparser
"We also found that USP20 could interact with MCL1 by removing its K48-linked ubiquitin chain."
IL1B affects USP20
| 1 2
IL1B phosphorylates USP20 on S333. 3 / 3
| 1 2

sparser
"In contrast, IL-1β-induced phosphorylation of USP20 on Ser334 was abolished in SMCs transfected with IRAK1-targeting siRNA ( xref , C and D )."

sparser
"Using in vitro phosphorylation with purified IRAK1 and siRNA-mediated gene silencing of IRAK1 in SMCs, we identified IRAK1 as a novel kinase for IL-1β–induced USP20 Ser334 phosphorylation."

reach
"We hypothesized that in the context of IL-1β signaling, perhaps other serine/threonine kinases, including IRAK1 might also phosphorylate USP20 Ser334."
| 2 1
E3_Ub_ligase ubiquitinates USP20.
| 2
E3_Ub_ligase ubiquitinates USP20. 2 / 2
| 2

reach
"To test whether hVDU2 can be ubiquitinated by pVHL E3 ubiquitin ligase complex, we performed ubiquitination assay in vitro and in vivo."

reach
"VDU2 itself can be ubiquitylated and degraded by the pVHL E3 ligase complex [32] ."
| 1

sparser
"When USP20 is thus phosphorylated, the association of USP20 with the E3 ubiquitin ligase HERC2 diminishes and, consequently, K48 polyubiquitination of USP20 diminishes."
DBNL affects USP20
3 |
3 |

No evidence text available

No evidence text available

No evidence text available
CD274 affects USP20
| 3
| 3

sparser
"Wang et al. demonstrated that USP20 directly interacts with PD-L1 [ xref ]."

sparser
"A recent study demonstrated that USP20 interacts with PD-L1 and deubiquitinates it, which can be regulated by a long non-coding RNA, tissue differentiation inducing non-protein coding RNA (TINCR)."

sparser
"Under physiological conditions, USP20PD-L1 interactions were validated using endogenous immunoprecipitation (Fig. xref )."
2 |
Sodium arsenite increases the amount of USP20. 2 / 2
2 |

No evidence text available

No evidence text available
P38 affects USP20
| 1 1
P38 phosphorylates USP20. 2 / 2
| 1 1

reach
"These observations introduce an additional layer of USP20 regulation, suggesting that p38 may phosphorylate USP20."

sparser
"These observations introduce an additional layer of USP20 regulation, suggesting that p38 may phosphorylate USP20."
VMP1 affects USP20
| 1 1
| 1 1

reach
"On the other hand, limited reports have indicated that VMP1 interacts with ubiquitin specific peptidase 20 (USP20) (Zhang et al., 2024) and USP9x (Grasso et al., 2011) at the ER membrane, which are responsible for its deubiquitination."

sparser
"On the other hand, limited reports have indicated that VMP1 interacts with ubiquitin specific peptidase 20 (USP20) ( xref ) and USP9x ( xref ) at the ER membrane, which are responsible for its deubiquitination."
USP33 affects NEURL4
| 2
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
USP21 affects USP20
2 |
2 |

No evidence text available

No evidence text available

reach
"USP20 and USP4 promote the stability of β-catenin, thus continuously activating the Wnt-β-catenin signaling pathway and mediating drug resistance in breast cancer and CRC ( Fig. 3 )."

reach
"67 A recent study reported that USP20 stabilizes β‐catenin and activates Wnt/β‐catenin signaling pathway, thereby promoting the migration, invasion, and proliferation of cancer cells."

reach
"Besides decreasing HMGCR and lipid biosynthesis, knock-out or silencing of Usp20 led to the increase of UCP1 and energy expenditure in BAT and iWAT (Figs."

reach
"By contrast, USP20 inhibition promotes HMGCR degradation, reduces lipid biosynthesis, enhances succinate production, and promotes heat generation."
USP20 affects glutathione
| 2
USP20 increases the amount of glutathione. 2 / 2
| 2

reach
"Consistent with the results identified above, USP20 WT and S132D/S368D reduced cell sensitivity to erastin and increased the GSH levels, while ferrous iron levels decreased and lipid ROS levels of HCC cells (Figures 6H–L)."

reach
"It is also found that USP20 depletion decreased the GSH levels in HCC cells, while increased lipid ROS levels and ferrous iron levels (Figures 2H–M and S2H–J)."
USP20 affects cholesterol
| 2
USP20 decreases the amount of cholesterol.
| 1
USP20 decreases the amount of cholesterol. 1 / 1
| 1

reach
"Deletion of USP20 attenuated hyperlipidemia and weight gain and was suggested as an attractive therapeutic target to reduce cholesterol levels [ 23 ]."
USP20 activates cholesterol.
| 1
| 1

reach
"USP20 links feeding induced cholesterol synthesis and energy expenditure."
USP20 affects cell death
| 1 1
| 1 1

reach
"As shown in Fig. 4 a, knockdown of USP20 importantly enhanced ABT-263-induced cell death in oesophageal cancer cells."

eidos
"Because our results suggest that the USP20-p62 axis is responsible for PKCzeta-mediated NF-kappaB activation for cell survival , USP20 depletion may promote cell death ."
USP20 affects cell cycle
| 2
| 2

reach
"In line with the clinical outcomes, we found that the overexpression of USP20 could, on one hand, suppress cell proliferation and delay G1-S cell cycle transition, and on the other hand, enhance autophagy in GC cells."

reach
"As shown in XREF_FIG, the silencing of USP20 expression accelerated the G1-S cell cycle transition which is accompanied by decrease in the percentage of G1 cells (down to 54.05%) and increase in the percentage of cells in the S phase (up to 37.62%), and the tendency was also consistent with MKN45-siUSP20 cells and BGC-823-siUSP20 cells (P < 0.05; XREF_FIG and XREF_FIG)."
USP20 affects Weight Gain
| 2
| 2

reach
"Suppression of USP20 significantly reduces weight gain, lipid levels in serum and liver, and improves insulin sensitivity and energy expenditure (122)."

reach
"The liver-specific deletion of Usp20 slowed weight gain without altering food intake (Fig. 5B, C)."
USP20 affects VMP1
| 1 1
| 1 1

reach
"On the other hand, limited reports have indicated that VMP1 interacts with ubiquitin specific peptidase 20 (USP20) (Zhang et al., 2024) and USP9x (Grasso et al., 2011) at the ER membrane, which are responsible for its deubiquitination."

sparser
"On the other hand, limited reports have indicated that VMP1 interacts with ubiquitin specific peptidase 20 (USP20) ( xref ) and USP9x ( xref ) at the ER membrane, which are responsible for its deubiquitination."
USP20 affects USP21
2 |
2 |

No evidence text available

No evidence text available
USP20 affects UBC
2 |
2 |

No evidence text available

No evidence text available
USP20 affects TRAF2
1 | 1
1 | 1

No evidence text available

sparser
"With these co-immunoprecipitation assays, we found that USP20 associated with the ubiquitin E3 ligases TRAF2, cIAP1, and two components of LUBAC xref : HOIL-1-interacting protein (HOIP, or RNF31) and SHARPIN (also known as SIPL1) ( xref )."
USP20 affects TCF4
| 1 1
| 1 1

reach
"Besides, β-catenin agonist (SKL2001) facilitated USP20 transcription by promoting TCF4 binding to USP20 promoter."

sparser
"Besides, β-catenin agonist (SKL2001) facilitated USP20 transcription by promoting TCF4 binding to USP20 promoter."
| 2
| 1

reach
"Silencing of USP20 increases proliferation in GC cells."
| 1

reach
"These finding revealed that USP20 could modulate GC cell proliferation."
USP20 affects STUB1
| 2
| 2

sparser
"Our study reveals a novel mechanism in which USP20 competitively interacts with STUB1 to stabilize CTSL and promote tumour progression."

sparser
"We demonstrate that USP20 competitively binds STUB1, inhibiting STUB1‐mediated ubiquitination and degradation of CTSL, thus stabilizing CTSL protein levels."
USP20 affects SELENBP1
| 1

sparser
"SELENBP1 also interacted with VDU2, a closely related isoform of VDU1 ( xref )."
USP20 decreases the amount of Reactive Oxygen Species. 2 / 2
| 2

reach
"It was found that USP20 knockdown increased lipid ROS levels and ferrous iron levels, while decreased the GSH levels."

reach
"Consistent with the results identified above, USP20 WT and S132D/S368D reduced cell sensitivity to erastin and increased the GSH levels, while ferrous iron levels decreased and lipid ROS levels of HCC cells (Figures 6H–L)."
USP20 affects RAB8A
| 2
| 2

sparser
"USP20-RAB8A signaling axis restricts pancreatic cancer progression by disrupting GLUT1 vesicular trafficking and inhibiting glucose uptake."

sparser
"Functional assays in vitro and in vivo validate the tumor-suppressive role of the USP20-RAB8A signaling axis."
USP20 affects OGD/R
| 2
USP20 inhibits OGD/R. 2 / 2
| 2

reach
"UCMSCs-derived exosomal SLIT2 activated TCF4 by promoting β-catenin nuclear translocation, which transcriptionally upregulated USP20 expression, thereby attenuating OGD/R-induced neuroncell damage and ultimately leading to inhibition of IS progression."

reach
"Intriguingly, USP20-inhibited inflammation and neuron damage were considerably abrogated by PTEN suppression in OGD/R-incubated cells."
USP20 affects NFKBIA
| 1 1
USP20 phosphorylates NFKBIA.
| 1
USP20 leads to the phosphorylation of NFKBIA. 1 / 1
| 1

reach
"Deletion of USP20 accelerates HSV-1-induced degradation of MITA and impairs phosphorylation of IRF3 and IkappaBalpha as well as subsequent induction of type I IFNs and proinflammatory cytokines after HSV-1 infection or cytoplasmic DNA challenge."
USP20 binds NFKBIA.
| 1
| 1

sparser
"For example, both β-arrestin2 xref and USP20 ( xref ) associate with IκBα; therefore, it is possible that a ternary complex of USP20, β-arrestin2 and IκBα facilitates deubiquitination of IκBα—a possibility made more plausible by the observation that β-arrestin2 and its homolog β-arrestin1 can inhibit IκBα degradation and NFκB signaling downstream of TNFR1. xref "
USP20 affects MYO1C
2 |
2 |

No evidence text available

No evidence text available
USP20 affects MYO1A
2 |
2 |

No evidence text available

No evidence text available
USP20 affects MDFI
2 |
2 |

No evidence text available

No evidence text available
USP20 affects MAP7D2
2 |
2 |

No evidence text available

No evidence text available
USP20 affects M
2 |
M binds USP20. 2 / 2
2 |

No evidence text available
| DOI

No evidence text available
| DOI
USP20 affects IKKalpha beta phosphorylation
| 2
USP20 activates IKKalpha beta phosphorylation. 2 / 2
| 2

eidos
"Furthermore , USP20 depletion significantly reduced IKKalpha / beta phosphorylation and delayed IkappaBalpha degradation in the presence of TNFalpha ( Figure 3E ) , which was similarly observed in p62-depleted HeLa cells ( Figure S1 ) ."

eidos
"In addition , USP20 depletion significantly decreased IKKalpha / beta phosphorylation and delayed IkappaBalpha degradation , resulting in the reduction of NF-kappaB-mediated cell survival ."
USP20 affects IHO1
2 |
2 |

No evidence text available

No evidence text available
USP20 affects Hyperplasia
| 2
| 2

reach
"Our findings reveal novel mechanisms regulating IL-1beta-induced proinflammatory signaling: by phosphorylating USP20 Ser334, IRAK1 diminishes the association of USP20 with TRAF6 and thus augments NFkappaB activation, SMC inflammation, and neointimal hyperplasia."

reach
"Altogether, these findings and Figure 4 suggest that phosphorylation of USP20 on Ser334 augments neointimal hyperplasia primarily by increasing inflammatory signaling and associated proliferation of SMCs.To model neointimal hyperplasia in vitro (16, 17), we assessed proliferation and migration of SMCs derived from WT and USP20-S334A mice."
USP20 affects FATE1
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2 |

No evidence text available

No evidence text available
| 2

reach
"The association of USP20 with its substrates triggers deubiquitinase activity; this association is regulated by phosphorylation of USP20 on Ser334 (mouse) or Ser333 (human)."

reach
"As shown in Figure 1F, we observed that USP20 was the most significantly upregulated DUB in Huh‐7/OXA cells."
USP20 affects Death
| 2
USP20 inhibits Death. 2 / 2
| 2

reach
"USP20 overexpression also alleviates the enhancement of inflammatory Iba-1 and NF-κB signaling in microglia, suggesting that USP20 reduces neuroinflammation and neuronal death."

reach
"Considering the contradictory role of PTEN by us and previous studies, more studies are still required to thoroughly explore the correlation between USP20 and PTEN during ischemic stroke progression o[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects DUSP
| 1 1
USP20 inhibits DUSP.
| 1
USP20 inhibits DUSP. 1 / 1
| 1

reach
"While the molecular details remain to be determined, these results show that the catalytic activity of USP15, USP11, and USP20 can be inhibited by targeting the DUSP rather than the catalytic domain i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 binds DUSP.
| 1
| 1

sparser
"20D2 binding to the USP20-DUSP ( Figure 5 (C)) could inhibit catalytic activity of these enzymes."
USP20 affects DIO2
1 1 |
1 1 |

No evidence text available

No evidence text available
USP20 affects CTAG1B
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No evidence text available

No evidence text available
USP20 affects CRC
| 2
USP20 activates CRC. 2 / 2
| 2

reach
"To examined whether USP20 promotes CRC progression, the cells were transiently transfected with USP20 plasmid or negative plasmid."

reach
"Overall, these results suggest that USP20 overexpression may promote CRC metastasis to promote cancer progression in CRC."
USP20 affects CRAMP1
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2 |

No evidence text available

No evidence text available
USP20 affects CNTN2
| 2
USP20 deubiquitinates CNTN2.
| 1
USP20 deubiquitinates CNTN2. 1 / 1
| 1

reach
"The ubiquitin specific peptidase USP20 (ubiquitin specific peptidase) deubiquitinates Tax-1 and suppresses IL-1beta- and Tax-1-induced NF-kappaB activation XREF_BIBR."
USP20 binds CNTN2.
| 1
| 1

reach
"Tax-1 interacts with NRP and optineurin and TAX1BP1, and with the ubiquitin specific peptidase USP20."
USP20 affects CASP3
| 2
USP20 inhibits CASP3. 2 / 2
| 2

reach
"Western blot analysis confirmed that USP20 over-expression significantly reduced Caspase-3 cleavage, while improved the expression of anti-apoptotic signal Bcl-2 in MCAO-operated mice ( Fig. 3 H and I[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Moreover, Caspase-3 activity caused by OGD/R was also greatly reduced by USP20 ( Fig. 3 L)."
USP20 affects BEND7
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2 |

No evidence text available

No evidence text available
USP20 affects Arrb2
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2 |

No evidence text available

No evidence text available
USP20 affects AR
| 2
USP20 ubiquitinates AR.
| 1
USP20 leads to the ubiquitination of AR. 1 / 1
| 1

reach
"Ubiquitination of the beta 2 AR is reversed by the deubiquitinases USP20 and USP33, which tonically associate with the beta 2 AR."
USP20 activates AR.
| 1
USP20 activates AR. 1 / 1
| 1

reach
"Therefore, USP20 phosphorylation/dephosphorylation may serve as a causative factor for disease progression, irreversible USP20 serine phosphorylation may contribute to βAR desensitization in heart fai[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects AGO2
| 1 1
| 1 1

reach
"USP20, TINCR, and miR-199a-5p were enriched in Ago2-containing miRNA containing ribonucleoprotein complexes relative to control IgG immunoprecipitates, suggesting that the Ago2 protein binds directly to USP20, TINCR, and miR-199a-5p in MDA-MB-231 cells (Fig. 4L–O)."

sparser
"USP20, TINCR, and miR-199a-5p were enriched in Ago2-containing miRNA containing ribonucleoprotein complexes relative to control IgG immunoprecipitates, suggesting that the Ago2 protein binds directly to USP20, TINCR, and miR-199a-5p in MDA-MB-231 cells (Fig. xref )."
USP20 affects ADRB2
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No evidence text available

No evidence text available
UBC affects USP20
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2 |

No evidence text available

No evidence text available
TRAF2 affects USP20
1 | 1
1 | 1

No evidence text available

sparser
"With these co-immunoprecipitation assays, we found that USP20 associated with the ubiquitin E3 ligases TRAF2, cIAP1, and two components of LUBAC xref : HOIL-1-interacting protein (HOIP, or RNF31) and SHARPIN (also known as SIPL1) ( xref )."
TCF4 affects USP20
| 1 1
| 1 1

reach
"Besides, β-catenin agonist (SKL2001) facilitated USP20 transcription by promoting TCF4 binding to USP20 promoter."

sparser
"Besides, β-catenin agonist (SKL2001) facilitated USP20 transcription by promoting TCF4 binding to USP20 promoter."
STUB1 affects USP20
| 2
| 2

sparser
"Our study reveals a novel mechanism in which USP20 competitively interacts with STUB1 to stabilize CTSL and promote tumour progression."

sparser
"We demonstrate that USP20 competitively binds STUB1, inhibiting STUB1‐mediated ubiquitination and degradation of CTSL, thus stabilizing CTSL protein levels."
SNAI2 affects USP20
| 2
| 2

sparser
"Another important regulating mechanism in breast cancer is the USP20-SNAI2 axis."

sparser
"Thus, their study suggested that USP20-SNAI2 axis may serve as a novel therapeutic target axis in breast cancer."
SELENBP1 affects USP20
| 1

sparser
"SELENBP1 also interacted with VDU2, a closely related isoform of VDU1 ( xref )."
RIPK1 affects USP20
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2 |

No evidence text available

No evidence text available
RETREG1 affects USP20
| 2
| 2

reach
"Moreover, the immunoprecipitation assay demonstrated an interaction between MYC-USP20 and FLAG-RETREG1, indicating their potential binding capability (Figure 3D)."

reach
"In addition, the endogenous interaction between USP20 and RETREG1 was confirmed through immunoprecipitation experiments using antibodies against RETREG1 (Figure 3E)."
RAB8A affects USP20
| 2
| 2

sparser
"USP20-RAB8A signaling axis restricts pancreatic cancer progression by disrupting GLUT1 vesicular trafficking and inhibiting glucose uptake."

sparser
"Functional assays in vitro and in vivo validate the tumor-suppressive role of the USP20-RAB8A signaling axis."
NEURL4 affects USP33
| 2
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
MYO1C affects USP20
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No evidence text available

No evidence text available
MYO1A affects USP20
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2 |

No evidence text available

No evidence text available
MDFI affects USP20
2 |
2 |

No evidence text available

No evidence text available
MAP7D2 affects USP20
2 |
2 |

No evidence text available

No evidence text available
M affects USP20
2 |
M binds USP20. 2 / 2
2 |

No evidence text available
| DOI

No evidence text available
| DOI
IHO1 affects USP20
2 |
2 |

No evidence text available

No evidence text available
GSK2643943A affects USP20
| 1 1
GSK2643943A inhibits USP20. 2 / 2
| 1 1

eidos
"For example , 8-mercapto-N - ( ( tetrahydro-3-furanyl ) methyl ) -4 - quinoline carboxamide , LND-57444 , VLX1570 , ML323 , ( ADC-01 , ADC-03 , HBX41108 , HBX19818 , P5091 , P22077 ) , 9 - ( ethoxyimino ) -9 H-indeno ( 1,2 - b ) pyrazine-2 ,3 - dicarbonitrile , WP1130 , Mitoxantrone and GSK2643943A are able to inhibit PSMD14 , UCHL1 , UCHL5 and USP14 , USP1 , USP7 , USP8 , USP9X , USP11 and USP20 , respectively ."

reach
"These results indicated that inhibition of USP20 by GSK2643943A could induce ferroptosis of HCC in vivo."
FOXP3 affects USP20
| 1 1
| 1

sparser
"We found that USP20 can bind and deubiquitinate FOXP3 (Fig.  xref )."
| 1

reach
"We found that USP20 can bind and deubiquitinate FOXP3 (Fig. 4d, e)."
FATE1 affects USP20
2 |
2 |

No evidence text available

No evidence text available
DNA Damage affects USP20
| 2
| 2

reach
"However, in cells depleted with USP20, such effect was not observed, suggesting that OXA induced DNA damage leads to USP20/SLC7A11‐dependent ferroptosis resistance in HCC cells."

reach
"These results suggest that OXA induced DNA damage leads to USP20/SLC7A11‐dependent ferroptosis resistance in HCC cells.3 DISCUSSION."
DIO2 affects USP20
1 1 |
1 1 |

No evidence text available

No evidence text available
CTAG1B affects USP20
2 |
2 |

No evidence text available

No evidence text available
CRAMP1 affects USP20
2 |
2 |

No evidence text available

No evidence text available
CCP110 affects USP33
| 2
| 2

sparser
"Thus, we sought to examine whether USP20 and USP33 interact with the CP110-Neurl4 complex to regulate centrosome function."

sparser
"We immunoprecipitated each protein from cell lysates and found that endogenous USP33 and USP20 specifically interact with CP110 and Neurl4, but not with two other CP110-interacting proteins, Cep97 and cyclin F ( xref )."
BEND7 affects USP20
2 |
2 |

No evidence text available

No evidence text available
Arrb2 affects USP20
2 |
2 |

No evidence text available

No evidence text available
ARRB2 affects USP20
1 | 1
1 | 1

No evidence text available

sparser
"The association of USP20 with β-arrestin2 was augmented when β-arrestin2 ubiquitination was prevented and reduced when β-arrestin2 ubiquitination was rendered constitutive."
AGO2 affects USP20
| 1 1
| 1 1

reach
"USP20, TINCR, and miR-199a-5p were enriched in Ago2-containing miRNA containing ribonucleoprotein complexes relative to control IgG immunoprecipitates, suggesting that the Ago2 protein binds directly to USP20, TINCR, and miR-199a-5p in MDA-MB-231 cells (Fig. 4L–O)."

sparser
"USP20, TINCR, and miR-199a-5p were enriched in Ago2-containing miRNA containing ribonucleoprotein complexes relative to control IgG immunoprecipitates, suggesting that the Ago2 protein binds directly to USP20, TINCR, and miR-199a-5p in MDA-MB-231 cells (Fig. xref )."
ADRB2 affects USP20
2 |
2 |

No evidence text available

No evidence text available
Urethane affects USP20
1 |
Urethane increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Trichloroethene increases the amount of USP20. 1 / 1
1 |

No evidence text available
Tetrachloromethane decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Sorafenib affects USP20
| 1
| 1

reach
"Interestingly, Sorafenib also induced degradation of USP20 in a proteasome-dependent manner ( Fig. 4 c)."
Protein kinase A-alpha affects USP20
| 1
Protein kinase A-alpha phosphorylates USP20 on S333. 1 / 1
| 1

reach
"beta1AR induced phosphorylation of USP20 Ser333 by protein kinase A-alpha (PKAalpha) was required for optimal USP20 mediated regulation of beta1AR lysosomal trafficking."
Potassium chromate increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Phenobarbital increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |

No evidence text available
1 |
Methamphetamine increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Hydrogen peroxide decreases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Hydrogen cyanide decreases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
Hsa-miR-26b-5p decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Folic acid affects USP20
1 |
Folic acid decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Flutamide affects USP20
1 |
Flutamide increases the amount of USP20. 1 / 1
1 |

No evidence text available
Fenthion affects USP20
1 |
Fenthion decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Estrogen affects USP20
1 |
Estrogen decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Cholesterol affects USP20
| 1
| 1

reach
"Endogenous cholesterol production can be greatly reduced by inhibiting USP20 function or reducing its expression in the liver."
Bisphenol A affects USP20
1 |
Bisphenol A decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Bis(2-ethylhexyl) phthalate increases the amount of USP20. 1 / 1
1 |

No evidence text available
1 |
1 |

No evidence text available
1 |
Benzo[a]pyrene increases the amount of USP20. 1 / 1
1 |

No evidence text available
ZXDB affects USP20
1 |
1 |

No evidence text available
ZNF620 affects USP20
1 |
1 |

No evidence text available
ZG16B affects USP20
1 |
1 |

No evidence text available
ZFYVE26 affects USP20
1 |
1 |

No evidence text available
YUH1 affects USP20
| 1
| 1

sparser
"Co‐immunoprecipitation assays revealed that the USP20UCH domain (amino acids 145–647) binds to CTSL (Figure xref )."
XPO7 affects USP20
1 |
1 |

No evidence text available
WASHC1 affects USP20
1 |
1 |

No evidence text available
1 |
Vehicle Emissions decreases the amount of USP20. 1 / 1
1 |

No evidence text available
VHL affects USP33
| 1
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
VAX1 affects USP20
1 |
1 |

No evidence text available
UbV.20D2 affects USP20
| 1
UbV.20D2 binds USP20. 1 / 1
| 1

reach
"To further validate the applicability of our DUSP UbV probes, we also tested if UbV.11D.2 binding to the USP11-DUSP ( Figure 5 (B)) or UbV.20D2 binding to the USP20-DUSP ( Figure 5 (C)) could inhibit [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP7 affects USP20
| 1
| 1

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
USP49 affects USP20
| 1
| 1

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
USP33 affects VHL
| 1
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
USP33 affects ARs
| 1
ARs binds USP33 and USP20. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP28 affects USP20
1 |
1 |

No evidence text available
USP20 affects β2-adrenergic receptor
| 1
USP20 leads to the ubiquitination of β2-adrenergic receptor. 1 / 1
| 1

reach
"USP33 and its homolog USP20 were shown to decrease β2-adrenergic receptor ubiquitination, which reduced receptor lysosomal degradation, and enhanced recycling and cellular resensitization (42)."
USP20 affects β2-AR
| 1
USP20 activates β2-AR. 1 / 1
| 1

reach
"It has been confirmed that USP20 can cause D2, HIF-1α, β2-AR, TRAF6, Tax and PKM2 to deubiquitinate, thereby regulating thyroid hormone activation, cell cycle regulation, inflammatory response, tumori[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects β2 adrenergic receptors
| 1
USP20 inhibits β2 adrenergic receptors. 1 / 1
| 1

reach
"Subsequently, they found that protein kinase A phosphorylates USP20 on serine 333, inhibits the trafficking of the substrate β2AR (β2 adrenergic receptors) and decreases degradation via autophagosomes [41]."
USP20 affects β -adrenergic receptor
| 1
USP20 ubiquitinates β -adrenergic receptor. 1 / 1
| 1

reach
"The Ser334 phosphorylation status of USP20 affects neither the binding of USP20 to the active-site directed probe Ub-VME nor USP20-mediated deubiquitination per se: whereas phosphorylation of USP20 on Ser334 reduces USP20-mediated deubiquitination of the β -adrenergic receptor, it increases USP20-mediated deubiquitination of the β -adrenergic receptor (10, 11)."
USP20 affects ubiquitin-proteasome
| 1
USP20 activates ubiquitin-proteasome. 1 / 1
| 1

reach
"These results indicated that USP20 directly interacts with YAP1 and increases its stability by preventing ubiquitin-proteasome pathway activity."
USP20 affects type I IFNs
| 1
USP20 inhibits type I IFNs. 1 / 1
| 1

reach
"Consistently, Usp20 -/- mice produce decreased type I IFNs and proinflammatory cytokines, exhibit increased susceptibility to lethal HSV-1 infection, and aggravated HSV-1 replication compared with Usp20 +/+ mice."

reach
"Taken together, these data suggest that phosphorylation of USP20 on Ser334 augments SMC proliferation and migration, particularly to agonists distinct from PDGF, like IL-1β."
USP20 affects signaling
| 1
USP20 inhibits signaling. 1 / 1
| 1

sparser
"Thus, RIPK1 itself appears to be a substrate of USP20—and, by directly deubiquitinating RIPK1, USP20 can inhibit TNFR1-triggered NFκB signaling."
USP20 affects signaling complex composed p62 PKCzeta RIPK1
| 1
USP20 activates signaling complex composed p62 PKCzeta RIPK1. 1 / 1
| 1

eidos
"To validate this possibility , we examined whether USP20 depletion disrupts formation of the signaling complex composed of p62 , PKCzeta and RIPK1 upon TNFalpha treatment ."

reach
"USP20 and USP4 promote the stability of β-catenin, thus continuously activating the Wnt-β-catenin signaling pathway and mediating drug resistance in breast cancer and CRC ( Fig. 3 )."

reach
"Cell cycle analysis indicated that USP20 depletion strongly induced cell cycle arrest, which was partially reversed by YAP1 overexpression in BC cells (Fig. 5A)."
USP20 affects post-endocytic trafficking autophagosomes
| 1
USP20 activates post-endocytic trafficking autophagosomes. 1 / 1
| 1

eidos
"However , solely USP20 was shown to promote the deubiquitination of beta2-ARs and their post-endocytic trafficking to autophagosomes ."
USP20 affects oxaliplatin
| 1
| 1

reach
"Ubiquitin specific peptidase 20 (USP 20) maintains the stability of SLC 7A 11 protein by removing K48 linked polyubiquitination of SLC 7A 11 protein at K30 and K37, inhibits the occurrence of ferroptosis in liver cancer, and increases the tolerance to oxaliplatin (64)."
USP20 affects neointimal hyperplasia caused carotid endothelial denudation transgenic mice model
| 1
USP20 inhibits neointimal hyperplasia caused carotid endothelial denudation transgenic mice model. 1 / 1
| 1

eidos
"Intriguingly , USP20 impedes TLR4-induced NF-kappaB activation by deubiquitinating TNF receptor-associated factor 6,147 whereas it inhibits IL-1R and TNF-triggered NF-kappaB activation by deubiquitinating receptor-interacting protein kinase 1.148 Consistent with the results of the in vitro experiments , USP20 decreased neointimal hyperplasia caused by carotid endothelial denudation in a transgenic mice model ."
USP20 affects navitoclax
| 1
| 1

reach
"Furthermore, the overexpression of USP20 rather than its catalytically inactive mutant USP20–C154S decreased the toxicity of ABT-263 in oesophageal cancer cells ( Fig. 4 b)."
| 1
| 1

reach
"Our experiments revealed that mutations in the FFAT motifs of USP20 disrupted its localization to the ER, underscoring the critical importance of these domains in this process (Figure 5A-H)."
USP20 affects ischemic stroke mice
| 1
USP20 inhibits ischemic stroke mice. 1 / 1
| 1

eidos
"USP20 mitigates ischemic stroke in mice by suppressing neuroinflammation and neuron death via regulating PTEN signal ."
| 1

reach
"By contrast, USP20 inhibition promotes HMGCR degradation, reduces lipid biosynthesis, enhances succinate production, and promotes heat generation."

reach
"USP20 facilitates RETREG1-VAPB association and enhances early autophagy protein recruitment for reticulophagy."
USP20 affects deubiquitination beta2-ARs
| 1
USP20 activates deubiquitination beta2-ARs. 1 / 1
| 1

eidos
"However , solely USP20 was shown to promote the deubiquitination of beta2-ARs and their post-endocytic trafficking to autophagosomes ."
USP20 affects cell migration invasion breast cancer
| 1
USP20 activates cell migration invasion breast cancer. 1 / 1
| 1

eidos
"USP4 and USP20 can promote the cell migration and invasion activities in breast cancer by inhibiting NFkappaB activation via deubiquitination of TRAF2 and TRAF6 [ 74 ] ."
USP20 affects cell growth
| 1
| 1

reach
"As shown in XREF_FIG, silencing of USP20 significantly promoted cell growth in monolayer culture, and further experimental results revealed that the proliferation ability of MGC-803-USP20 cells were lower than that of MGC-803-NC cells (XREF_FIG)."

reach
"In their study, they demonstrated USP20 regulated the stability of Claspin and thus modulating the activation of the cell cycle checkpoint."
USP20 affects beta-arrestins
| 1
USP20 leads to the deubiquitination of beta-arrestins. 1 / 1
| 1

reach
"Deubiquitination of beta-arrestins is also regulated by agonist activation of the beta2AR and other GPCRs and is mediated by USP20 and USP33 and is important for beta, arrestin, and beta2AR complex disassembly, lysosomal sorting and signaling [XREF_BIBR, XREF_BIBR] (XREF_FIG)."
USP20 affects behavior
| 1
| 1

eidos
"Notably , suppressing PTEN with its specific inhibitor dramatically abolished the function of USP20 to ameliorate neuroinflammation and neuron death induced by OGD / R. Collectively , our results illustrated that USP20 could effectively mitigate the severity of cerebral ischemic stroke and improve behavior deficits in MCAO-operated mice , and identified the USP20 / PTEN axis as a promising therapeutic target for ischemic stroke treatment ."
USP20 affects apoptosis induced cycloheximide TNFalpha TAK1
| 1
USP20 activates apoptosis induced cycloheximide TNFalpha TAK1. 1 / 1
| 1

eidos
"Furthermore , depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor ."
USP20 affects andB
| 1
USP20 activates andB. 1 / 1
| 1

reach
"We observed that USP20 depletion remarkably inhibited the oncosphere formation of Huh‐7, Hep3B and HCC primary cells (Figures S2K, S5A, andB)."
USP20 affects activity
| 1
USP20 inhibits activity. 1 / 1
| 1

sparser
"To identify potential substrates of USP20 and elucidate mechanisms by which USP20 inhibits TNF-evoked NFκB activity, we immunoprecipitated HA-tagged USP20 from HEK-293 cells and immunoblotted for endogenous proteins that constitute TNFR1-activated signaling intermediates."
USP20 affects activation
| 1
USP20 inhibits activation. 1 / 1
| 1

sparser
"Furthermore, host DUBs that interact with HTLV-1Tax oncoprotein were investigated, and consequently ubiquitin-specific peptidase USP20 was found to deubiquitinate Tax and inhibit Tax-induced NF-κB activation [ xref ]."

reach
"In addition, a novel role for USP20 in promoting actin cytoskeleton organization and cell migration was revealed and linked to its regulation of ERK3 expression."
USP20 affects ZXDB
1 |
1 |

No evidence text available
USP20 affects ZNF620
1 |
1 |

No evidence text available
USP20 affects ZG16B
1 |
1 |

No evidence text available
USP20 affects ZFYVE26
1 |
1 |

No evidence text available
USP20 affects YUH1
| 1
| 1

sparser
"Co‐immunoprecipitation assays revealed that the USP20UCH domain (amino acids 145–647) binds to CTSL (Figure xref )."
USP20 affects XPO7
1 |
1 |

No evidence text available
USP20 affects WASHC1
1 |
1 |

No evidence text available
USP20 affects VAX1
1 |
1 |

No evidence text available
USP20 affects UbV.20D2
| 1
UbV.20D2 binds USP20. 1 / 1
| 1

reach
"To further validate the applicability of our DUSP UbV probes, we also tested if UbV.11D.2 binding to the USP11-DUSP ( Figure 5 (B)) or UbV.20D2 binding to the USP20-DUSP ( Figure 5 (C)) could inhibit [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects USP7
| 1
| 1

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
USP20 affects USP49
| 1
| 1

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
USP20 affects USP33, and VHL
| 1
VHL binds USP33 and USP20. 1 / 1
| 1

sparser
"In conclusion, our previously published results and current data indicate that the interaction between pVHL and VDU1 or VDU2 mediates the ubiquitination and degradation of these two proteins."
USP20 affects USP28
1 |
1 |

No evidence text available
USP20 affects USP15
1 |
1 |

No evidence text available
USP20 affects UBA52
1 |
1 |

No evidence text available
USP20 affects Tax-induced NF-kappaB activation
| 1
USP20 inhibits Tax-induced NF-kappaB activation. 1 / 1
| 1

eidos
"For example , USP11 negatively regulates TNFalpha-induced NF-kappaB activation associated with IkappaBalpha and attenuates IkappaBalpha degradation [ 34 ] ; USP20 deubiquitinates TRAF6 and suppresses interleukin 1beta ( IL-1beta ) - and Tax-induced NF-kappaB activation [ 40 ] ; Katrin et al. showed that USP15 regulates IkappaBalpha / NF-kappaB by deubiquitinylation IkappaBalpha [ 44 ] ; and USP31 inhibits TNFalpha , CD40 , TRAF2 , TRAF6 and IKKbeta-mediated NF-kappaB activation [ 45 ] ."
USP20 affects TUBB
1 |
1 |

No evidence text available
USP20 affects TTC23L
1 |
1 |

No evidence text available
USP20 affects TRIM23
1 |
1 |

No evidence text available
USP20 affects TRIB3
1 |
1 |

No evidence text available
USP20 affects TPM3
1 |
1 |

No evidence text available
USP20 affects TNNI1
1 |
1 |

No evidence text available
USP20 affects TNFalpha-mediated
| 1
USP20 binds TNFalpha-mediated. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
USP20 affects TNFalpha-induced NF-kappaB activation regarding PKCzeta
| 1
USP20 activates TNFalpha-induced NF-kappaB activation regarding PKCzeta. 1 / 1
| 1

eidos
"Based on our data that USP20 is responsible for NF-kappaB-mediated cell survival through stabilizing p62 , we also suggest that USP20 depletion causes the blockade of TNFalpha-induced NF-kappaB activation regarding PKCzeta and thus may accelerate cell death pathways ."
USP20 affects TNFRSF25
| 1
| 1

reach
"USP20 also promotes metastasis of CRC cells and is closely correlated with several immune checkpoint genes, including ADORA2A (adenosine A2a receptor), CD160 (cluster of differentiation 160), CD27 (cluster of differentiation 27), and TNFRSF25 (TNF receptor superfamily member 25)[79]."
USP20 affects TLR4
| 1
| 1

reach
"Among them, Usp8, Usp9x, and Usp20 bound to TLR4 most tightly (Figure 1J)."
USP20 affects TGM3
1 |
1 |

No evidence text available
USP20 affects TEAD4
1 |
1 |

No evidence text available
USP20 affects SYTL4
1 |
1 |

No evidence text available
USP20 affects SUN2
1 |
1 |

No evidence text available
USP20 affects STIM1
1 |
1 |

No evidence text available
USP20 affects STAMBP
| 1
| 1

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
USP20 affects SRGAP2B
1 |
1 |

No evidence text available
USP20 affects SPTAN1
1 |
1 |

No evidence text available
USP20 affects SPRY2
1 |
1 |

No evidence text available
USP20 affects SPAST
1 |
1 |

No evidence text available
USP20 affects SOX2
| 1
USP20 increases the amount of SOX2. 1 / 1
| 1

reach
"In addition, depletion of USP20 significantly decreased the expression of pluripotent transcription factors Nanog, Sox2, c‐Myc, and Oct4 (Figures S5C and D)."
USP20 affects SNAI1
| 1
USP20 increases the amount of SNAI1. 1 / 1
| 1

reach
"Knockdown of USP20 suppressed cell proliferation, migration, and invasion and decreased the levels of SOX4, N-cadherin, Snail, and Slug but increased the E-cadherin level[53]."
USP20 affects SLC39A10
1 |

No evidence text available
USP20 affects SHARPIN
1 |
1 |

No evidence text available
USP20 affects SEC62
1 |
1 |

No evidence text available
USP20 affects S phase
| 1
USP20 activates S phase. 1 / 1
| 1

reach
"Low expression of USP20 significantly promoted cell proliferation and accelerated the transition from G1 to S phase of the cell cycle [60]."
USP20 affects RPS5
1 |
1 |

No evidence text available
USP20 affects RPS18
1 |
1 |

No evidence text available
USP20 affects RPS10
1 |
1 |

No evidence text available
USP20 affects RPL7A
1 |
1 |

No evidence text available
USP20 affects RPL3
1 |
1 |

No evidence text available
USP20 affects RPL13
1 |
1 |

No evidence text available
USP20 affects RGPD6
1 |
1 |

No evidence text available
USP20 affects RFC4
1 |
1 |

No evidence text available
USP20 affects RFC3
1 |
1 |

No evidence text available

reach
"Moreover, ectopic expression of Usp20 in smooth muscle cells significantly reduced aortic atherosclerosis in LdlrKO mice, while the introduction of the dominant negative form of USP20 increased atherosclerotic plaques [216]."
USP20 affects PSMD6
1 |
1 |

No evidence text available
USP20 affects PRKN
| 1
USP20 phosphorylates PRKN on S65. 1 / 1
| 1

sparser
"Subsequently, we examined the phosphorylation of parkin in HEK293 cells and found that USP20 significantly increased the phosphorylation of parkin at S65, compared to treatment with CCCP alone. (Fig.  xref F)."
USP20 affects PRKCZ
1 |
1 |

No evidence text available
USP20 affects POLDIP3
1 |
1 |

No evidence text available
USP20 affects PLK1
1 |
1 |

No evidence text available
USP20 affects PLEKHA7
1 |
1 |

No evidence text available
USP20 affects PKCzeta-RIPK1-p62
| 1
USP20 binds PKCzeta-RIPK1-p62. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
USP20 affects PJA1
1 |
1 |

No evidence text available
USP20 affects PD-L1 inhibitors
| 1
USP20 deubiquitinates PD-L1 inhibitors. 1 / 1
| 1

reach
"Consequently, USP20 deubiquitinates PD-L1, increasing BC cell resistance to PD-L1 inhibitors [213]."
USP20 affects PBX4
1 |
1 |

No evidence text available
USP20 affects NIPA1
1 |
1 |

No evidence text available
USP20 affects NFASC
| 1
USP20 inhibits NFASC. 1 / 1
| 1

reach
"These data suggest that preventing USP20 Ser334 phosphorylation reduces NFκB activation in the context of neointimal hyperplasia.Because neointimal hyperplasia integrally involves SMC proliferation (16), we asked whether USP20-S334A reduced SMC proliferation in the injured carotid arteries."
USP20 affects NF-κBp65 subunit
| 1
Mutated USP20 leads to the phosphorylation of NF-κBp65 subunit. 1 / 1
| 1

reach
"In addition, the introduction of an enzymatically inactive mutant of Usp20 substantially increased the phosphorylation of the NF-κBp65 subunit and upregulated the expression of vascular cell adhesion molecule 1 (VCAM-1) in mouse carotid arteries [215]."
USP20 affects NANOG
| 1
USP20 increases the amount of NANOG. 1 / 1
| 1

reach
"In addition, depletion of USP20 significantly decreased the expression of pluripotent transcription factors Nanog, Sox2, c‐Myc, and Oct4 (Figures S5C and D)."
USP20 affects N4BP3
1 |
1 |

No evidence text available
USP20 affects MYO1D
1 |
1 |

No evidence text available
USP20 affects MYL6
1 |
1 |

No evidence text available
USP20 affects MYL12B
1 |
1 |

No evidence text available
USP20 affects MYH7
| 1
USP20 activates MYH7. 1 / 1
| 1

reach
"In this mouse model, they find that USP20 deubiquitinates and prevents the protein degradation of Myosin Heavy Chain 7 (MYH7) through lysine-48 polyubiquitination [158]."
USP20 affects MYH10
1 |
1 |

No evidence text available
USP20 affects MYC
| 1
| 1

sparser
"Employing a stable cell line expressing FLAG-GFP-RETREG1 and utilizing FLAG for pulldown of the conjugated ubiquitin signal, we observed an increase in the conjugated ubiquitin signal on RETREG1 upon USP20 knockdown ( xref ), aligning with the data obtained from treating cells with the USP20 inhibitor GSK2643943A. Moreover, the immunoprecipitation assay demonstrated an interaction between MYC-USP20 and FLAG-RETREG1, indicating their potential binding capability ( xref )."
USP20 affects MRPL28
1 |
1 |

No evidence text available
USP20 affects MEOX2
1 |
1 |

No evidence text available
USP20 affects MCCD1
1 |
1 |

No evidence text available
USP20 affects MCAO-operated mice
| 1
USP20 inhibits MCAO-operated mice. 1 / 1
| 1

eidos
"Notably , suppressing PTEN with its specific inhibitor dramatically abolished the function of USP20 to ameliorate neuroinflammation and neuron death induced by OGD / R. Collectively , our results illustrated that USP20 could effectively mitigate the severity of cerebral ischemic stroke and improve behavior deficits in MCAO-operated mice , and identified the USP20 / PTEN axis as a promising therapeutic target for ischemic stroke treatment ."
USP20 affects MBD3L1
1 |
1 |

No evidence text available
USP20 affects LMO7
1 |
1 |

No evidence text available
USP20 affects LMNA
1 |
1 |

No evidence text available
USP20 affects LC3-II
| 1
USP20 inhibits LC3-II. 1 / 1
| 1

reach
"Interestingly, the single knockdown of USP20 triggered an increased accumulation of LC3-II (Fig. 4B, lane 1 compared with lane 3)."
USP20 affects KPRP
1 |
1 |

No evidence text available
USP20 affects KCNF1
1 |
1 |

No evidence text available
USP20 affects K48-
| 1
USP20 deubiquitinates K48- on K63. 1 / 1
| 1

reach
"In our study, we found that USP20 deubiquitinates K48- and K63-linked ubiquitin chains on RETREG1 and promotes its stabilization."
USP20 affects JUP
1 |
1 |

No evidence text available
USP20 affects Interferon
| 1
| 1

reach
"XREF_BIBR This study showed that USP18 or USP20 deficiency significantly inhibited HSV-I or cytosolic DNA activation of both NF-kappaB and IRF3, and type-I IFN and proinflammatory cytokine expression."
USP20 affects Infections
| 1
| 1

reach
"The complementation of STING into Usp20 (-/-) cells remarkably restores HSV-1-triggered signaling and inhibits HSV-1 infection (92)."
USP20 affects ITGB3BP
1 |
1 |

No evidence text available
USP20 affects IRAK1BP1
1 |

No evidence text available
USP20 affects IGLV2-11
1 |

No evidence text available
USP20 affects HSPA5
1 |
1 |

No evidence text available
USP20 affects HSF2BP
1 |
1 |

No evidence text available
USP20 affects HSD17B11
1 |

No evidence text available
USP20 affects HRNR
1 |
1 |

No evidence text available
USP20 affects HNRNPCL1
1 |

No evidence text available
USP20 affects HIF-1alpha target
| 1
USP20 activates HIF-1alpha target. 1 / 1
| 1

eidos
"As a consequence of this interaction , USP20 induces the expression of HIF-1alpha target genes , such as vascular endothelial growth factor ( VEGF ) ."
USP20 affects HIF-1alpha [
| 1
USP20 binds HIF-1alpha [. 1 / 1
| 1

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."
USP20 affects HIF)-1alpha
| 1
USP20 deubiquitinates HIF)-1alpha. 1 / 1
| 1

reach
"Indeed, USP20 deubiquitinates and stabilizes its associated protein, hypoxia inducible factor (HIF)-1alpha, resulting in the promotion of cancer cell proliferation, invasion, and metastasis [XREF_BIBR, XREF_BIBR]."
USP20 affects HERC2P2
1 |
1 |

No evidence text available
USP20 affects HAND2
1 |
1 |

No evidence text available
USP20 affects H2BC21
1 |
1 |

No evidence text available

reach
"USP20 promotes genome stability and suppresses xenograft tumor growth."
USP20 affects GTF2IRD1
1 |

No evidence text available
USP20 affects GPT2
1 |
1 |

No evidence text available
USP20 affects GPRASP3
1 |
1 |

No evidence text available
USP20 affects GPRASP2
1 |
1 |

No evidence text available
USP20 affects GOT
| 1
USP20 activates GOT. 1 / 1
| 1

reach
"Simultaneously, si-Usp20 induced a slight increase in serum AST (supplemental Fig. S3F), indicating that long-term high-dose siRNA treatment may cause some liver damage, which should be investigated further."
USP20 affects FOXP1
| 1
| 1

sparser
"Chip‐qPCR showed an association of Foxp1 with the promoter of Usp20 (Figure  xref ; Table xref , Supporting Information) and luciferase assay confirmed that Foxp1 expression vector could dose‐dependently repress the promoter of Usp20 (Figure  xref ) containing FXOP1 binding sites in NIH‐3T3 cells."
USP20 affects FLG2
1 |
1 |

No evidence text available
USP20 affects ERAP1
1 |
1 |

No evidence text available
USP20 affects EPAS1
1 |
1 |

No evidence text available
USP20 affects ENKD1
1 |
1 |

No evidence text available
USP20 affects EMD
1 |
1 |

No evidence text available
USP20 affects ELOVL5
1 |
1 |

No evidence text available
USP20 affects EIF3C
1 |
1 |

No evidence text available
USP20 affects EEF1A1
1 |
1 |

No evidence text available
| 1

sparser
"When USP20 is thus phosphorylated, the association of USP20 with the E3 ubiquitin ligase HERC2 diminishes and, consequently, K48 polyubiquitination of USP20 diminishes."
USP20 affects DUSP16
1 |
1 |

No evidence text available
USP20 affects DUSP1
| 1
| 1

sparser
"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
USP20 affects DSP
1 |
1 |

No evidence text available
USP20 affects DSG1
1 |
1 |

No evidence text available

reach
"59 , 60 , 61 , 62 , 63 , 64 USP20 mediates ATR‐mediated DNA damage response through the stabilization of Claspin."
USP20 affects DDR
| 1
USP20 phosphorylates DDR. 1 / 1
| 1

sparser
"These results establish the important role of USP20 phosphorylation in ATR-dependent DDR."
USP20 affects CTSD
1 |
1 |

No evidence text available
USP20 affects CLAPSIN
| 1
USP20 binds CLAPSIN. 1 / 1
| 1

reach
"We then wanted to determine the functional significance of the interaction between USP20 and CLAPSIN."
USP20 affects CDK2AP1
1 |
1 |

No evidence text available
USP20 affects CALM1
1 |
1 |

No evidence text available
USP20 affects C1QTNF2
1 |
1 |

No evidence text available
| 1

reach
"USP20 was shown to promote breast tumor metastasis, and proliferation of oral squamous carcinoma cells."
USP20 affects BRPF1
1 |
1 |

No evidence text available
USP20 affects BCAM
| 1
USP20 activates BCAM. 1 / 1
| 1

reach
"Lu et al. demonstrated the post-prandial increase in insulin and the glucose concentration stimulates mTORC1 to phosphorylate the deubiquitylase ubiquitin-specific peptidase 20 (USP20) at S132 and S134, which is recruited to the HMGCR complex and antagonizes its degradation (Lu et al., 2020)."
USP20 affects BC
| 1
USP20 activates BC. 1 / 1
| 1

reach
"Rescue experiments were performed to explore whether USP20 modulates BC progression through YAP1."

reach
"In contrast, the overexpression of Usp26 decreased the number of AP + colonies, while overexpression of Usp20 increased the number of AP + colonies."
USP20 affects AZGP1
1 |
1 |

No evidence text available
USP20 affects AURKA
1 |
1 |

No evidence text available
USP20 affects ATR-CLASPIN-CHK1
| 1
USP20 activates ATR-CLASPIN-CHK1. 1 / 1
| 1

reach
"Our results demonstrated that USP20 positively regulates the ATR-CLASPIN-CHK1 signaling during replication stress."
USP20 affects ATP6AP2
1 |
1 |

No evidence text available
USP20 affects ASCL1
1 |
1 |

No evidence text available
USP20 affects ARs
| 1
ARs binds USP33 and USP20. 1 / 1
| 1

sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
USP20 affects ARG1
1 |
1 |

No evidence text available
USP20 affects ALB
1 |
1 |

No evidence text available
USP20 affects AGR2
1 |
1 |

No evidence text available
USP20 affects ADRB1
| 1
USP20 inhibits ADRB1. 1 / 1
| 1

reach
"For example, ubiquitin carboxyl-terminal hydrolase 20 (USP20) knockout reduces beta1-adrenergic receptor (beta1AR)-induced cardiac contractility and relaxation."
USP20 affects ADORA2A
| 1
USP20 activates ADORA2A. 1 / 1
| 1

reach
"USP20 also promotes metastasis of CRC cells and is closely correlated with several immune checkpoint genes, including ADORA2A (adenosine A2a receptor), CD160 (cluster of differentiation 160), CD27 (cluster of differentiation 27), and TNFRSF25 (TNF receptor superfamily member 25)[79]."
USP15 affects USP20
1 |
1 |

No evidence text available
UBA52 affects USP20
1 |
1 |

No evidence text available
TUBB affects USP20
1 |
1 |

No evidence text available
TTC23L affects USP20
1 |
1 |

No evidence text available
TRIM23 affects USP20
1 |
1 |

No evidence text available
TRIB3 affects USP20
1 |
1 |

No evidence text available
TPM3 affects USP20
1 |
1 |

No evidence text available
TNNI1 affects USP20
1 |
1 |

No evidence text available
TNFalpha-mediated affects USP20
| 1
USP20 binds TNFalpha-mediated. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
TNF affects USP20
| 1
| 1

sparser
"Co-immunoprecipitation experiments revealed that USP20 associates with several components of the TNF receptor-1 signaling pathway including receptor-interacting protein kinase 1 (RIPK1), a critical checkpoint in TNF-induced NFκB activation and inflammation."
TLR4 affects USP20
| 1
| 1

reach
"Among them, Usp8, Usp9x, and Usp20 bound to TLR4 most tightly (Figure 1J)."
TGM3 affects USP20
1 |
1 |

No evidence text available
TEAD4 affects USP20
1 |
1 |

No evidence text available
Smoke affects USP20
1 |
Smoke decreases the amount of USP20. 1 / 1
1 |

No evidence text available
Si-Usp20-1 affects USP20
| 1
Si-Usp20-1 inhibits USP20. 1 / 1
| 1

reach
"Si-Usp20-1 was able to effectively decrease Usp20 mRNA and protein levels in primary hepatocytes (supplemental Fig. S1C, D)."
SYTL4 affects USP20
1 |
1 |

No evidence text available
SUN2 affects USP20
1 |
1 |

No evidence text available
STIM1 affects USP20
1 |
1 |

No evidence text available
STAMBP affects USP20
| 1
| 1

reach
"Additionally, we conducted Co-IP experiments in NRK-52E cells to verify the binding situation, and the results demonstrated varying degrees of binding between Usp49, Stambp, Usp8, Usp9x, Usp33, Usp20, and Usp7 with TLR4."
SRGAP2B affects USP20
1 |
1 |

No evidence text available
SPTAN1 affects USP20
1 |
1 |

No evidence text available
SPRY2 affects USP20
1 |
1 |

No evidence text available
SPAST affects USP20
1 |
1 |

No evidence text available
SLC39A10 affects USP20
1 |

No evidence text available
SHARPIN affects USP20
1 |
1 |

No evidence text available
SEC62 affects USP20
1 |
1 |

No evidence text available
RPS5 affects USP20
1 |
1 |

No evidence text available
RPS18 affects USP20
1 |
1 |

No evidence text available
RPS10 affects USP20
1 |
1 |

No evidence text available
RPL7A affects USP20
1 |
1 |

No evidence text available
RPL3 affects USP20
1 |
1 |

No evidence text available
RPL13 affects USP20
1 |
1 |

No evidence text available
RORC affects USP20
| 1
RORC leads to the phosphorylation of USP20. 1 / 1
| 1

reach
"In the post-prandial phase, high glucose and insulin levels lead to TOR activation and phosphorylation of USP20."
RGPD6 affects USP20
1 |
1 |

No evidence text available
RFC4 affects USP20
1 |
1 |

No evidence text available
RFC3 affects USP20
1 |
1 |

No evidence text available
Particulate Matter decreases the amount of USP20. 1 / 1
1 |

No evidence text available
PSMD6 affects USP20
1 |
1 |

No evidence text available
PRKCZ affects USP20
1 |
1 |

No evidence text available
POLDIP3 affects USP20
1 |
1 |

No evidence text available
PLK1 affects USP20
1 |
1 |

No evidence text available
PLEKHA7 affects USP20
1 |
1 |

No evidence text available
PKCzeta-RIPK1-p62 affects USP20
| 1
USP20 binds PKCzeta-RIPK1-p62. 1 / 1
| 1

isi
"Furthermore, depletion of USP20 disrupts formation of the atypical PKCzeta-RIPK1-p62 complex required for TNFalpha-mediated NF-kappaB activation and significantly increases the apoptosis induced by TNFalpha plus cycloheximide or TNFalpha plus TAK1 inhibitor."
PJA1 affects USP20
1 |
1 |

No evidence text available
PDIA3 affects USP20
| 1
PDIA3 leads to the phosphorylation of USP20. 1 / 1
| 1

reach
"Moreover, ubiquitin specific peptidase 20 (USP20) improved FTO level through inhibiting FTO degradation while PDIA3 increased FTO level by enhancing USP20 phosphorylation during osteogenic differentiation of MC3T3-E1 cells, suggesting a positive feedback regulatory loop between PDIA3 and FTO."
PBX4 affects USP20
1 |
1 |

No evidence text available
NIPA1 affects USP20
1 |
1 |

No evidence text available
NFkappaB affects USP20
| 1

sparser
"Co-immunoprecipitation showed that USP20 interacts with several NF-κB signaling components, including receptor-interacting protein kinase 1 (RIPK1) [ xref ]."
NFKBIA affects USP20
| 1
| 1

sparser
"For example, both β-arrestin2 xref and USP20 ( xref ) associate with IκBα; therefore, it is possible that a ternary complex of USP20, β-arrestin2 and IκBα facilitates deubiquitination of IκBα—a possibility made more plausible by the observation that β-arrestin2 and its homolog β-arrestin1 can inhibit IκBα degradation and NFκB signaling downstream of TNFR1. xref "
N4BP3 affects USP20
1 |
1 |

No evidence text available
MYO1D affects USP20
1 |
1 |

No evidence text available
MYL6 affects USP20
1 |
1 |

No evidence text available
MYL12B affects USP20
1 |
1 |

No evidence text available
MYH10 affects USP20
1 |
1 |

No evidence text available
MYC affects USP20
| 1
| 1

sparser
"Employing a stable cell line expressing FLAG-GFP-RETREG1 and utilizing FLAG for pulldown of the conjugated ubiquitin signal, we observed an increase in the conjugated ubiquitin signal on RETREG1 upon USP20 knockdown ( xref ), aligning with the data obtained from treating cells with the USP20 inhibitor GSK2643943A. Moreover, the immunoprecipitation assay demonstrated an interaction between MYC-USP20 and FLAG-RETREG1, indicating their potential binding capability ( xref )."
MRPL28 affects USP20
1 |
1 |

No evidence text available
MEOX2 affects USP20
1 |
1 |

No evidence text available
MCCD1 affects USP20
1 |
1 |

No evidence text available
MBD3L1 affects USP20
1 |
1 |

No evidence text available
LMO7 affects USP20
1 |
1 |

No evidence text available
LMNA affects USP20
1 |
1 |

No evidence text available
KPRP affects USP20
1 |
1 |

No evidence text available
KCNF1 affects USP20
1 |
1 |

No evidence text available
JUP affects USP20
1 |
1 |

No evidence text available
ITGB3BP affects USP20
1 |
1 |

No evidence text available
IRAK1BP1 affects USP20
1 |

No evidence text available
IL1 affects USP20
| 1
IL1 phosphorylates USP20 on S333. 1 / 1
| 1

reach
"In contrast, IL-1β-induced phosphorylation of USP20 on Ser334 was abolished in SMCs transfected with IRAK1-targeting siRNA (Fig. 9, C and D)."
IGLV2-11 affects USP20
1 |

No evidence text available
HSPA5 affects USP20
1 |
1 |

No evidence text available
HSF2BP affects USP20
1 |
1 |

No evidence text available
HSD17B11 affects USP20
1 |

No evidence text available
HRNR affects USP20
1 |
1 |

No evidence text available
HNRNPCL1 affects USP20
1 |

No evidence text available
HIF-1alpha [ affects USP20
| 1
USP20 binds HIF-1alpha [. 1 / 1
| 1

reach
"For example, VHL interacting deubiquitinating enzyme 2 (VDU2) interacts with HIF-1alpha and specifically deubiquitinates and stabilizes HIF-1alpha [XREF_BIBR]."
HERC2P2 affects USP20
1 |
1 |

No evidence text available
HERC2 affects CLSPN
| 1
| 1

sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
HAND2 affects USP20
1 |
1 |

No evidence text available
H2BC21 affects USP20
1 |
1 |

No evidence text available
Gentamicins affects USP20
1 |
Gentamicins increases the amount of USP20. 1 / 1
1 |

No evidence text available
Gastrectomy affects USP20
| 1
Gastrectomy decreases the amount of USP20. 1 / 1
| 1

reach
"Our research demonstrated that SG downregulates the expression level of USP20, which attenuates lipid accumulation in vitro."
GTF2IRD1 affects USP20
1 |

No evidence text available
GPT2 affects USP20
1 |
1 |

No evidence text available
GPRASP3 affects USP20
1 |
1 |

No evidence text available
GPRASP2 affects USP20
1 |
1 |

No evidence text available
FOXP1 affects USP20
| 1
| 1

sparser
"Chip‐qPCR showed an association of Foxp1 with the promoter of Usp20 (Figure  xref ; Table xref , Supporting Information) and luciferase assay confirmed that Foxp1 expression vector could dose‐dependently repress the promoter of Usp20 (Figure  xref ) containing FXOP1 binding sites in NIH‐3T3 cells."
FLG2 affects USP20
1 |
1 |

No evidence text available
ERAP1 affects USP20
1 |
1 |

No evidence text available
EPAS1 affects USP20
1 |
1 |

No evidence text available
ENKD1 affects USP20
1 |
1 |

No evidence text available
EMD affects USP20
1 |
1 |

No evidence text available
ELOVL5 affects USP20
1 |
1 |

No evidence text available
EIF3C affects USP20
1 |
1 |

No evidence text available
EEF1A1 affects USP20
1 |
1 |

No evidence text available
DUSP16 affects USP20
1 |
1 |

No evidence text available
DUSP1 affects USP20
| 1
| 1

sparser
"DNA sequences encoding the following residues from each USP were cloned into a pET-21b vector using Gibson Assembly 45 : USP4-DUSP, 8–129; USP15-DUSP, 1–125; USP11-DUSP, 71–191; USP20-DUSP1, 687–780; [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
DUSP affects USP20
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sparser
"20D2 binding to the USP20-DUSP ( Figure 5 (C)) could inhibit catalytic activity of these enzymes."
DSP affects USP20
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No evidence text available
DSG1 affects USP20
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No evidence text available
CTSD affects USP20
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No evidence text available
CNTN2 affects USP20
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reach
"Tax-1 interacts with NRP and optineurin and TAX1BP1, and with the ubiquitin specific peptidase USP20."
CLSPN affects HERC2, and USP20
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sparser
"Co-immunoprecipitation assays revealed that both HERC2 and CLASPIN were present in the USP20 immunocomplex (Figure xref ) and both USP20 and CLASPIN were in the HERC2 immunocomplex (Figure  xref ), suggesting that HERC2, USP20 and CLASPIN form a complex."
CLAPSIN affects USP20
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USP20 binds CLAPSIN. 1 / 1
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reach
"We then wanted to determine the functional significance of the interaction between USP20 and CLAPSIN."
CDK2AP1 affects USP20
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No evidence text available
CALM1 affects USP20
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No evidence text available
C1QTNF2 affects USP20
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No evidence text available
BRPF1 affects USP20
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No evidence text available
BCAM affects USP20
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BCAM phosphorylates USP20 on S132. 1 / 1
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reach
"Lu et al. [28] found that elevated insulin and glucose concentrations stimulate mTORC1 to phosphorylate USP20 at S132 and S134; USP20 is then recruited to HMGCR to stabilize its structure and antagoni[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
Arrestins affects USP20
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Arrestins deubiquitinates USP20. 1 / 1
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reach
"Arrestins also recruit deubiquitinating enzymes USP33 and USP20, important for recycling and resensitisation to the beta2-adrenergic receptor."
AZGP1 affects USP20
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No evidence text available
AURKA affects USP20
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No evidence text available
ATP6AP2 affects USP20
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No evidence text available
ATM/ATR affects USP20
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ATM/ATR phosphorylates USP20. 1 / 1
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rlimsp
"Using an antibody against consensus ATM/ATR phosphorylation sites (anti-phospho-SQ/TQ), we found that phosphorylation of USP20 at SQ/TQ motifs following HU or UV treatment and USP20 phosphorylation was blocked by caffeine (Figure 5B and C)."
ATM affects USP20
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ATM phosphorylates USP20 on S132. 1 / 1
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sparser
"For example, the phosphorylation of USP20 at Ser132 and Ser368 by ataxia telangiectasia and Rad3-related (ATR) activation enhances its stability and then confers resistance to ferroptosis via the deubiquitination of SLC7A11."
ASCL1 affects USP20
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No evidence text available
ARs affects USP33
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ARs binds USP33 and USP20. 1 / 1
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sparser
"Plasma membrane-localized β 2 ARs can bind to two closely related DUBs, USP33 and USP20 (also known as VDU2)."
ARG1 affects USP20
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No evidence text available
ALB affects USP20
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No evidence text available
AGR2 affects USP20
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No evidence text available
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No evidence text available
2-hydroxypropanoic acid decreases the amount of USP20. 1 / 1
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No evidence text available
17alpha-ethynylestradiol decreases the amount of USP20. 1 / 1
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No evidence text available
1,2-dimethylhydrazine decreases the amount of USP20. 1 / 1
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No evidence text available
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reach
"VDU1, but not VDU2, is markedly increased in brown adipocytes by norepinephrine or cold exposure, further amplifying the increase in D2 activity that results from catecholamine stimulated de novo synthesis."