IndraLab

Statements

PRPF8 is modified

19 | 30 1

PRPF8 is phosphorylated.

18 | 22 1

PRPF8 is phosphorylated. 10 / 21

| 20 1

➶

sparser

"We suggest that phosphorylation of eIF-4E is more complicated than previously recognized and that hyperphosphorylation of eIF-4E and p220 recruits more p220 into the protein complex that associates with mRNA caps."

8444875

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sparser

"Treatment of MDCK-1 cells with the tyrosine kinase inhibitor herbimycin A reverted the cell phenotype to a more MDCK-like morphology in culture, with a concomitant reduction in the tyrosine phosphorylation predominantly of p220."

8886986

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sparser

"When incubations were carried out in the presence of Mn" and low levels of Zn^, the phosphorylation of P180 and P220 was inhibited while the phosphorylation of P20 was not affected, thus sugg[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

4062988

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sparser

"Phosphorylation of both the p25 and p220 subunits of eIF-4F is stimulated typically by 2.5-5-fold, with a 2-4-fold increase in phosphorylation of eIF-4B and eIF-3 p120."

2191953

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sparser

"Moreover, anti-TNF-alpha antibodies inhibit LPS-stimulated phosphorylation of eIF-4E and p220 by 43% (+/- 6%) and 50% (+/- 5%), respectively."

1345341

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sparser

"In reactions carried out in the absence of Zn^, resistant membranes contained phosphorylated P20, P180 and P220 (Fig. 2D) and proteins of similar molecular weights were either absent or present in[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

4062988

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sparser

"Because insulin-like growth factor I stimulation of glucose uptake is not inhibited in cells with A/K1018 receptors while pp220 and pp170 phosphorylation is inhibited, it is unlikely that either pp220 or pp170 are involved in mediating the stimulation of glucose transport."

2153140

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sparser

"Partial digests of phosphorylated p180 and p220 produced with chymotrypsin or V8 protease reveal that each protein has a distinct phosphopeptide pattern."

4063966

➶

sparser

"To facilitate histone genes transcription during cell cycle cyclin E/CDK2 phosphorylates p220(NPAT) [ xref ]."

26059433

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sparser

"P220, P180 and P20 were not phosphorylated in membranes from cells that had reverted to drug sensitivity, suggesting that these three proteins may have an important role in adriamycin resistance."

4062988

PRPF8 is phosphorylated on Y254. 2 / 2

2 |

➶

hprd

No evidence text available

20068231

➶

hprd

No evidence text available

18452278

PRPF8 is phosphorylated on serine. 2 / 2

| 2

➶

sparser

"Phosphoamino acid analysis of the isolated proteins demonstrated that both P220 and P180 were phosphorylated only at serine residues (Fig. 3, A and B)."

1 |

➶

hprd

No evidence text available

20068231

PRPF8 is ubiquitinated.

| 8

PRPF8 is ubiquitinated. 8 / 8

| 8

➶

sparser

"Shortly afterwards, it was also demonstrated that the E3 ligase named Prp19 increases the affinity between the ubiquitinated Prp3 protein and Prp8, thus contributing to stabilization of the triple snRNP."

| PMC

➶

sparser

"A model was proposed in which inhibition of Brr2 by the ubiquitinated Prp8 leads to spliceosome assembly and stabilization of the triple snRNP (U4/U6–U5)."

| PMC

➶

sparser

"Finally, we show that the conserved splicing factor Prp8 is ubiquitinated within purified triple snRNPs."

18425143

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sparser

"It was further demonstrated that Prp8 is ubiquitinated within the tri-snRNP ( xref )."

20462492

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"Important questions that can be addressed using our minimal system include determining whether the Prp8-CTF also promotes Brr2-depending unwinding of U2 and U6, and whether helicase activity is inhibited by Prp8 ubiquitination."

19098916

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sparser

"In addition to recent studies suggesting that ubiquitination of Prp8 regulates tri-snRNP disassembly, xref , xref there are other indications that ubiquitin or ubiquitin-like proteins (such as Sumo and Hub1) play additional roles in splicing."

20672149

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sparser

"Prp8, a key RNA-binding splicing factor, is ubiquitylated to suppress the U4/U6 snRNAs unwinding activity of Brr2 helicase and promote spliceosome assembly."

27203182

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sparser

"Staley and Sontheimer uncovered a regulatory mechanism, namely that the activity of Brr2 is negatively regulated by ubiquitination of Prp8 (ref. xref )."

19098916

PRPF8 is acetylated.

1 |

PRPF8 is acetylated on K1463. 1 / 1

1 |

➶

hprd

No evidence text available

19608861

PRPF8 affects EFTUD2

9 | 5 24

PRPF8 binds EFTUD2.

9 | 4 24

PRPF8 binds EFTUD2. 10 / 20

9 | 3 8

➶

biogrid

No evidence text available

9774689

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biogrid

No evidence text available

27173435

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sparser

"To determine whether the interactions between R2TP and PRPF8 or EFTUD2 occur at an early cytoplasmic stage, we implemented a corecruitment assay using a fusion of RPAP3 with a fragment of p54, which targets the fusion protein to cytoplasmic P-bodies ( xref )."

28515276

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biogrid

No evidence text available

25263594

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sparser

"We also identified several U5 chaperones such as AAR2, ZNHIT2, RUVBL1, RUVBL2, and ECD and a putative chaperone EAPP that has been shown to interact with U5 proteins PRPF8 and EFTUD2 xref , xref , xref ."

34131137

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sparser

"The HSP90/R2TP machinery binds unassembled cytoplasmic forms of PRPF8 and EFTUD2."

28515276

➶

reach

"An intermediate complex is formed with the recruitment of PRPF8 and EFTUD2 to the R2TP and HSP90 complex along with AAR2, ZNHIT2, and other assembly factors."

28884116

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biogrid

No evidence text available

22939629

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sparser

"SNRNP200 is a large nuclear protein of 200 kDa, which interacts extensively with U5-specific proteins EFTUD2 (hSnu114) and Prpf8."

27302685

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biogrid

No evidence text available

26344197

PRPF8 binds EFTUD2 and SNRNP200. 7 / 7

| 1 6

➶

sparser

"AAR2, ZNHIT2, and the HSP90/R2TP complex associate with the U5-specific proteins EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

"Remarkably, the GFP-ZNHIT2 SILAC IP revealed a strong association of this factor with EFTUD2, PRPF8, and SNRNP200."

28515276

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sparser

"Notably, depletion of the spliceosome components BRR2 and EFTUD2, which interact directly with PRPF8 to form the U5 snRNP [ xref ], most strongly phenocopy PRPF8 deficiency (Fig. xref ; Additional file xref b, c)."

26392272

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sparser

"Similarly, in humans, yeast two-hybrid and in vitro binding assays have demonstrated physical interactions between EFTUD2, SNRNP200 and PRPF8 proteins ( xref , xref ), and these interactions have been confirmed by recent cryo-EM structures of the human U4/U6."

33584830

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sparser

"Importantly, our results suggest that components of Complex B, and more specifically BRR2 and EFTUD2, which interact directly with PRPF8 to form the U5 snRNP complex [ xref ], phenocopy PRPF8 most strongly."

26392272

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sparser

"Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200."

34131137

➶

reach

"In addition to the PRPF8, EFTUD2, and SNRNP200 complex, our proteomics analysis as well as previous analyses found that SNW1 was in complex with another splicing subcomplex, PRP19 complex."

25450007

AAR2 binds PRPF8 and EFTUD2. 2 / 2

| 2

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sparser

"These results support the idea that PRPF8, AAR2, and EFTUD2 form an assembly intermediate in human cells and that some of the mutants are trapped at this stage."

| 1

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sparser

"Association of RUVBL2 with either EFTUD2 or PRPF8 was greatly compromised attesting to the efficiency of the knockdown ( xref , xref )."

28561026

PRPF8 activates EFTUD2.

| 1

PRPF8 activates EFTUD2. 1 / 1

| 1

➶

reach

"Depletion of U5 proteins and PRPF8 RP mutation both increase the association of PRPF8 and EFTUD2 with R2TP."

28515276

PRPF8 affects SNRNP200

7 | 5 19

PRPF8 binds SNRNP200.

7 | 2 16

PRPF8 binds SNRNP200. 10 / 13

7 | 1 5

➶

biogrid

No evidence text available

23704370

➶

biogrid

No evidence text available

22939629

➶

biogrid

No evidence text available

27173435

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biogrid

No evidence text available

16723661

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sparser

"Moreover, they also report that a loss of PHF6 results in increased genomic instability at rDNA genes and a cell cycle delay at G2/M. While this was the first indication of a nucleolar function for PHF6, it has also been reported to interact with PRPF8 and SNRNP200, both constituents of the U4/U6-U5 tri-snRNP pre-mRNA splicing ribonucleoprotein complex [ xref ]."

26103525

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sparser

"In addition, a small but reproducible reduction of SNRNP200 and PRPF8 binding was also observed in the case of the Δ201–250 deletion mutant."

34131137

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

17317632

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biogrid

No evidence text available

16723661

➶

reach

"The conservative missense variant p.PRPF8-Arg2310Lys that is not altering the global charge of the C-terminal tail, and variants located at the basis of the C-terminal tail show milder clinical phenotypes, in accordance with functional data on PRPF8 and SNRNP200 interactions in yeast."

29087248

PRPF8 binds EFTUD2 and SNRNP200. 7 / 7

| 1 6

➶

sparser

"AAR2, ZNHIT2, and the HSP90/R2TP complex associate with the U5-specific proteins EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

"Remarkably, the GFP-ZNHIT2 SILAC IP revealed a strong association of this factor with EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

26392272

➶

sparser

33584830

➶

sparser

26392272

➶

sparser

"Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200."

| 1 3

PRPF8 inhibits SNRNP200. 4 / 4

| 1 3

➶

sparser

"In humans, phosphorylation of Prp6 that occurs after the tri-snRNP being integrated into the B-complex may release the inhibition of Brr2 by Prp8 and is important for spliceosomal B-complex activation [ xref , xref ]."

27058959

➶

sparser

"A model was proposed in which inhibition of Brr2 by the ubiquitinated Prp8 leads to spliceosome assembly and stabilization of the triple snRNP (U4/U6–U5)."

| PMC

➶

sparser

"Given the mechanistic analogy between histone mRNA processing and splicing, we thus envisage that SLBP ubiquitylation may suppress hPrp43 helicase activity in histone mRNA processing similar to Prp8 u[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

27203182

➶

reach

"The RNase H domain of PRPF8 inhibits loading of SNRNP200 to U4 snRNA, and a C-terminal part of PRPF8 modulates the SNRNP200 activity for the unwinding of U4/U6 snRNA duplex XREF_BIBR - XREF_BIBR."

25450007

PRPF8 activates SNRNP200.

| 2

PRPF8 activates SNRNP200. 2 / 2

| 2

➶

reach

"Although it has been demonstrated that the PRPF8 RNase H domain inhibits SNRNP200 activity and GTP bound EFTUD2 and that the C-terminus of PRPF8 induces the activation of SNRNP200 XREF_BIBR - XREF_BIBR, the detailed molecular mechanisms of the activation of SNRNP200 remains to be determined."

25450007

➶

reach

"The RNase H domain of PRPF8 inhibits loading of SNRNP200 to U4 snRNA, and a C-terminal part of PRPF8 modulates the SNRNP200 activity for the unwinding of U4/U6 snRNA duplex XREF_BIBR - XREF_BIBR."

25450007

SNRNP200 affects PRPF8

7 | 3 16

SNRNP200 binds PRPF8.

7 | 2 16

PRPF8 binds SNRNP200. 10 / 13

7 | 1 5

➶

biogrid

No evidence text available

23704370

➶

biogrid

No evidence text available

22939629

➶

biogrid

No evidence text available

27173435

➶

biogrid

No evidence text available

16723661

➶

sparser

26103525

➶

sparser

"In addition, a small but reproducible reduction of SNRNP200 and PRPF8 binding was also observed in the case of the Δ201–250 deletion mutant."

34131137

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

17317632

➶

biogrid

No evidence text available

16723661

➶

reach

29087248

PRPF8 binds EFTUD2 and SNRNP200. 7 / 7

| 1 6

➶

sparser

"AAR2, ZNHIT2, and the HSP90/R2TP complex associate with the U5-specific proteins EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

"Remarkably, the GFP-ZNHIT2 SILAC IP revealed a strong association of this factor with EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

26392272

➶

sparser

33584830

➶

sparser

26392272

➶

sparser

"Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200."

34131137

➶

reach

"In addition to the PRPF8, EFTUD2, and SNRNP200 complex, our proteomics analysis as well as previous analyses found that SNW1 was in complex with another splicing subcomplex, PRP19 complex."

25450007

PRPF8 binds SNRNP40, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"Because Snrnp40 is tightly bound to Prpf8, Eftud2, and Snrnp200, it is likely that Snrnp40 also participates and facilitates these processes."

31427773

PRPF8 binds COPS5 and SNRNP200. 1 / 1

| 1

➶

sparser

33584830

PRPF8 binds LAP3, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"The proteomic analysis of the GFP IPs showed a strong association of PRPF8-LAP with its partners EFTUD2 and SNRNP200, and conversely, GFP-EFTUD2 strongly bound PRPF8 and SNRNP200."

28515276

PRPF8 binds SNRNP200 and TSSC4. 1 / 1

| 1

➶

sparser

34131137

PRPF31 binds PRPF8, PRPF3, SNRNP200, and ATXN3. 1 / 1

| 1

➶

sparser

22819011

SNRNP200 deubiquitinates PRPF8.

| 1

SNRNP200 deubiquitinates PRPF8. 1 / 1

| 1

➶

reach

"Following de-ubiquitination of Prp8, Brr2 is free to promote unwinding of U4/U6, resulting in activation of the spliceosome [48]."

| PMC

PRPF8 affects AAR2

5 | 2 9

AAR2 binds PRPF8. 10 / 12

5 | 2 5

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

22939629

➶

sparser

"Based on the structure of the AAR2-PRPF8 RH complex, the importance of the interacting regions and residues was probed and AAR2 variants were designed that failed to stably bind PRPF8 in vitro."

36322420

➶

sparser

"Nevertheless, the GFP-AAR2 IP purified only a few other U5 proteins, which is in agreement with the idea that AAR2 binds PRPF8 mostly before its association with U5 snRNA."

28515276

➶

sparser

"The results showed that the interactions of PRPF8 with R2TP and AAR2 increased in the absence of PIH1D1."

28515276

➶

sparser

"Yeast and human PRPF8 associate with the chaperone AAR2, which helps to properly fold PRPF8 xref – xref ."

34131137

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

28514442

➶

reach

"Nevertheless, the GFP-AAR2 IP purified only a few other U5 proteins, which is in agreement with the idea that AAR2 binds PRPF8 mostly before its association with U5 snRNA."

28515276

➶

sparser

"CK2α1 and SGK2 kinases can abrogate the interaction between the spliceosomal proteins AAR2 and PRPF8, and NEK6 kinase was found to mediate the estrogen receptor (ERα) interaction with 14-3-3 proteins."

35225431

AAR2 binds PRPF8 and EFTUD2. 2 / 2

| 2

➶

sparser

"These results support the idea that PRPF8, AAR2, and EFTUD2 form an assembly intermediate in human cells and that some of the mutants are trapped at this stage."

28515276

➶

sparser

"Collectively, these data provide strong support for the idea that PRPF8 associates with EFTUD2 and AAR2 early in its assembly pathway."

28515276

AAR2 binds PRPF8, EFTUD2, and RP. 1 / 1

| 1

➶

sparser

"To test whether this was also the case in human cells, we tested the association of PRPF8 RP mutants with AAR2 and EFTUD2."

28515276

AAR2 binds ECD and PRPF8. 1 / 1

| 1

➶

sparser

"We characterized the interactions between R2TP, PRPF8, ZNHIT2, ECD and AAR2 proteins."

34951455

SART3 affects PRPF8

1 | 4 7

SART3 binds PRPF8. 10 / 11

1 | 4 6

➶

sparser

"Furthermore, miR-10b- U6 chimeras have been detected in the cross-linked and ligated SART3 and PRPF8 complexes, similar to the Ago complexes (Fig. xref b) indicative of the miR-10b interaction with U6 snRNP in the splicing complex."

35033060

➶

sparser

"Conversely, Ago2 and pan-Ago were immunoprecipitated in the SART3 and PRPF8 complexes (Supplementary Fig. xref b-e)."

35033060

➶

biogrid

No evidence text available

22939629

➶

sparser

"We further demonstrate that miR-10b modulates U6 N-6-adenosine methylation and pseudouridylation, U6 binding to splicing factors SART3 and PRPF8, and regulates U6 stability, conformation, and levels."

35033060

➶

reach

"At least two pieces of evidence provide support for the nuclear miR-10b maturation scenario: first, the finding of Ago2 and, more recently, of Dicer in the nucleus of specific cells, including glioma (Suppementary Fig. 5a) and [12, 56–58], and second, the co-IP of the spliceosomal SART3 and PRPF8 complexes with Ago2, the core endonucleolytic components of RISC that efficiently pools-down the entire miR-10b population from glioma cells."

35033060

➶

sparser

"At least two pieces of evidence provide support for the nuclear miR-10b maturation scenario: first, the finding of Ago2 and, more recently, of Dicer in the nucleus of specific cells, including glioma (Suppementary Fig. xref a) and [ xref , xref – xref ], and second, the co-IP of the spliceosomal SART3 and PRPF8 complexes with Ago2, the core endonucleolytic components of RISC that efficiently pools-down the entire miR-10b population from glioma cells."

35033060

➶

sparser

"These results indicate that miR-10b interferes with U6 incorporation into SART3 and PRPF8 snRNPs."

35033060

➶

sparser

"Both U6 and miR-10b have been significantly enriched in the spliceosomal SART3 and PRPF8 complexes (Fig. xref a and Supplementary Fig. xref a, b)."

35033060

➶

reach

"Conversely, Ago2 and pan-Ago were immunoprecipitated in the SART3 and PRPF8 complexes (Supplementary Fig. 5b-e)."

35033060

➶

reach

"Both U6 and miR-10b have been significantly enriched in the spliceosomal SART3 and PRPF8 complexes (Fig. 3a and Supplementary Fig. 4a, b)."

35033060

SART3 binds PRPF8 and AGO2. 1 / 1

| 1

➶

sparser

"At least two pieces of evidence provide support for the nuclear miR-10b maturation scenario: first, the finding of Ago2 and, more recently, of Dicer in the nucleus of specific cells, including glioma (Suppementary Fig. xref a) and [ xref , xref – xref ], and second, the co-IP of the spliceosomal SART3 and PRPF8 complexes with Ago2, the core endonucleolytic components of RISC that efficiently pools-down the entire miR-10b population from glioma cells."

35033060

SNRNP40 affects PRPF8

9 | 1

PRPF8 binds SNRNP40. 9 / 9

9 |

➶

biogrid

No evidence text available

26186194

➶

biogrid

No evidence text available

17353931

➶

biogrid

No evidence text available

22939629

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

9774689

➶

biogrid

No evidence text available

16723661

➶

biogrid

No evidence text available

16723661

➶

biogrid

No evidence text available

9774689

➶

biogrid

No evidence text available

28514442

PRPF8 binds SNRNP40, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"Because Snrnp40 is tightly bound to Prpf8, Eftud2, and Snrnp200, it is likely that Snrnp40 also participates and facilitates these processes."

31427773

SNRNP200 affects EFTUD2

| 1 8

PRPF8 binds EFTUD2 and SNRNP200. 7 / 7

| 1 6

➶

sparser

"AAR2, ZNHIT2, and the HSP90/R2TP complex associate with the U5-specific proteins EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

"Remarkably, the GFP-ZNHIT2 SILAC IP revealed a strong association of this factor with EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

26392272

➶

sparser

33584830

➶

sparser

26392272

➶

sparser

"Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200."

34131137

➶

reach

"In addition to the PRPF8, EFTUD2, and SNRNP200 complex, our proteomics analysis as well as previous analyses found that SNW1 was in complex with another splicing subcomplex, PRP19 complex."

25450007

PRPF8 binds SNRNP40, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"Because Snrnp40 is tightly bound to Prpf8, Eftud2, and Snrnp200, it is likely that Snrnp40 also participates and facilitates these processes."

31427773

PRPF8 binds LAP3, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"The proteomic analysis of the GFP IPs showed a strong association of PRPF8-LAP with its partners EFTUD2 and SNRNP200, and conversely, GFP-EFTUD2 strongly bound PRPF8 and SNRNP200."

28515276

PRPF8 affects PRPF3

3 | 2 4

PRPF8 binds PRPF3.

3 | 1 4

PRPF8 binds PRPF3. 6 / 6

3 | 1 2

➶

biogrid

No evidence text available

20595234

➶

sparser

"It is interesting to note that the interaction of PRPF8 with ubiquitinated PRPF3 is regulated by the deubiquitinating enzyme USP4, and that loss of USP4 prevents the correct splicing of mRNAs including those for α-tubulin xref ."

26167768

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

22939629

➶

sparser

"We also found that PRPF19 regulates MDM4 splicing by promoting the physical interaction of other splicing factors, PRPF3 and PRPF8, which are key components of the core spliceosome, U4/U6."

34144037

➶

reach

"The SNRNP200, PRPF8, PRPF3, and PRPF31 gene products are all triple-snRNP components, 15,31 and PAP1 binds PRPF3 to negatively regulate splicing, 32,49 suggesting a common mechanism for pathogenesis."

19878916

PRPF31 binds PRPF8, PRPF3, SNRNP200, and ATXN3. 1 / 1

| 1

➶

sparser

sparser

"Drosophila and human ECD proteins bind to PRPF8, an essential scaffolding component of the spliceosome machinery ( xref , xref – xref ), suggesting a role of ECD in splicing."

34670863

➶

sparser

"Notably, mammalian PRPF8 also interacts with ECD ( xref ), and another study found ECD, PRPF8, and R2TP subunits in a single complex ( xref )."

33941617

➶

biogrid

No evidence text available

22939629

➶

biogrid

No evidence text available

28514442

➶

sparser

"Such a role was consistent with our’ and others’ findings that ECD associates with the core spliceosome scaffold protein PRPF8 ( xref – xref )."

34670863

➶

biogrid

No evidence text available

28561026

➶

sparser

"The interaction of human ECD with human PRPF8 has been confirmed [ xref ]."

28492064

AAR2 binds ECD and PRPF8. 1 / 1

| 1

➶

sparser

"We characterized the interactions between R2TP, PRPF8, ZNHIT2, ECD and AAR2 proteins."

34951455

EFTUD2 affects SNRNP200

| 1 8

PRPF8 binds EFTUD2 and SNRNP200. 7 / 7

| 1 6

➶

sparser

"AAR2, ZNHIT2, and the HSP90/R2TP complex associate with the U5-specific proteins EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

"Remarkably, the GFP-ZNHIT2 SILAC IP revealed a strong association of this factor with EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

26392272

➶

sparser

33584830

➶

sparser

26392272

➶

sparser

"Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200."

34131137

➶

reach

"In addition to the PRPF8, EFTUD2, and SNRNP200 complex, our proteomics analysis as well as previous analyses found that SNW1 was in complex with another splicing subcomplex, PRP19 complex."

25450007

PRPF8 binds SNRNP40, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"Because Snrnp40 is tightly bound to Prpf8, Eftud2, and Snrnp200, it is likely that Snrnp40 also participates and facilitates these processes."

31427773

PRPF8 binds LAP3, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"The proteomic analysis of the GFP IPs showed a strong association of PRPF8-LAP with its partners EFTUD2 and SNRNP200, and conversely, GFP-EFTUD2 strongly bound PRPF8 and SNRNP200."

28515276

ECD affects PRPF8

4 | 5

ECD binds PRPF8. 8 / 8

4 | 4

➶

biogrid

No evidence text available

26711270

➶

sparser

"Drosophila and human ECD proteins bind to PRPF8, an essential scaffolding component of the spliceosome machinery ( xref , xref – xref ), suggesting a role of ECD in splicing."

34670863

➶

sparser

"Notably, mammalian PRPF8 also interacts with ECD ( xref ), and another study found ECD, PRPF8, and R2TP subunits in a single complex ( xref )."

33941617

➶

biogrid

No evidence text available

22939629

➶

biogrid

No evidence text available

28514442

➶

sparser

"Such a role was consistent with our’ and others’ findings that ECD associates with the core spliceosome scaffold protein PRPF8 ( xref – xref )."

34670863

➶

biogrid

No evidence text available

28561026

➶

sparser

"The interaction of human ECD with human PRPF8 has been confirmed [ xref ]."

28492064

AAR2 binds ECD and PRPF8. 1 / 1

| 1

➶

sparser

"We characterized the interactions between R2TP, PRPF8, ZNHIT2, ECD and AAR2 proteins."

34951455

PRPF3 affects PRPF8

3 | 1 4

PRPF8 binds PRPF3. 6 / 6

3 | 1 2

➶

biogrid

No evidence text available

20595234

➶

sparser

26167768

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

22939629

➶

sparser

"We also found that PRPF19 regulates MDM4 splicing by promoting the physical interaction of other splicing factors, PRPF3 and PRPF8, which are key components of the core spliceosome, U4/U6."

34144037

➶

reach

19878916

PRPF31 binds PRPF8, PRPF3, SNRNP200, and ATXN3. 1 / 1

| 1

➶

sparser

22819011

SF3B1 binds U2AF1, PRPF8, and PRPF3. 1 / 1

| 1

➶

sparser

28703423

SERPINB2 affects PRPF8

2 1 | 2 2

PRPF8 binds SERPINB2. 7 / 7

2 1 | 2 2

➶

hprd

No evidence text available

15346200

➶

reach

"PAI-2 could bind PRPF8 C-terminal in both the inside and outside of nuclear."

15346200

➶

biogrid

No evidence text available

15346200

➶

biogrid

No evidence text available

15346200

➶

sparser

"PAI-2 could bind PRPF8 C-terminal in both the inside and outside of nuclear."

15346200

➶

sparser

"Co-immunoprecipitation experiments confirmed that PAI-2 could interact with PRPF8 in vivo."

15346200

➶

reach

"Co-immunoprecipitation experiments confirmed that PAI-2 could interact with PRPF8 in vivo."

15346200

EFTUD2 affects PRPF8, and SNRNP200

| 1 6

PRPF8 binds EFTUD2 and SNRNP200. 7 / 7

| 1 6

➶

sparser

"AAR2, ZNHIT2, and the HSP90/R2TP complex associate with the U5-specific proteins EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

"Remarkably, the GFP-ZNHIT2 SILAC IP revealed a strong association of this factor with EFTUD2, PRPF8, and SNRNP200."

28515276

➶

sparser

26392272

➶

sparser

33584830

➶

sparser

26392272

➶

sparser

"Specifically, TSSC4 interacts with U5-specific proteins PRPF8, EFTUD2 and SNRNP200."

34131137

➶

reach

"In addition to the PRPF8, EFTUD2, and SNRNP200 complex, our proteomics analysis as well as previous analyses found that SNW1 was in complex with another splicing subcomplex, PRP19 complex."

25450007

PRPF8 affects cell population proliferation

| 1 5

PRPF8 activates cell population proliferation.

| 1 4

PRPF8 activates cell population proliferation. 5 / 5

| 1 4

➶

reach

"Meanwhile, circRNA-UBAP2 represses apoptosis and contributes to the proliferation of ovarian cancer by miR-382-5p/PRPF8 axis (Xu et al., 2020)."

34122108

➶

reach

"CircRNA-UBAP2 can be used as a ceRNA of spongy miR-382-5p in ovarian cancer, increasing the expression level of PRPF8, promoting the proliferation of ovarian cancer cells, and inhibiting cell apoptosis (Xu et al., 2020)."

34504842

➶

eidos

"( C ) The expression location of Prp8 was explored via immunofluorescence ( D ) CCK8 assay revealed that loss of Prp8 reduced cell proliferation ."

32547101

➶

reach

"Consequently, circRNA-UBAP2 upregulated expression level of PRPF8 by sponged miR-382-5p acted as a ceRNA, promoted proliferation and inhibited apoptosis in ovarian cancer cells."

32690086

➶

reach

"We show that PRPF8 lesions lead to neomorphic spliceosomal activity and increased cellular proliferation resulting in a distinct phenotype of aggressive myeloid malignancies with increased RS."

24781015

PRPF8 inhibits cell population proliferation.

| 1

PRPF8 inhibits cell population proliferation. 1 / 1

| 1

➶

reach

"Unlike SF3B1, XREF_BIBR, XREF_BIBR PRPF8 knockdown leads to increased cellular proliferation and increased clonogenicity."

24781015

PRPF8 affects PRP8

| 6

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

"Thus the Prp8p-Aar2p complex that accumulates in the prp8-rp mutants is nuclear and therefore, unlike the situation with ΔNLS-Prp8p, has the possibility to interact with Brr2p but does so with reduced efficiency as a consequence of the poor association of Brr2p with Prp8-rp mutant proteins."

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

PRPF8 binds CDC40 and PRP8. 1 / 1

| 1

➶

sparser

"On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction."

10628969

SRRM2 binds PRPF8 and PRP8. 1 / 1

| 1

➶

sparser

"Complementary chemical cross-linking experiments reveal reciprocal protein footprints between the interacting Prp8 and Cwc21 proteins, identifying the conserved cwf21 domain in Cwc21p as a Prp8p binding site."

19854871

PRPF8 binds PRP8. 1 / 1

| 1

➶

sparser

"K63-linked ubiquitylation of PRP31 by PRP19 regulates its association with PRP8 and the U5 snRNP."

35205626

PRPF8 binds EFTUD2 and PRP8. 1 / 1

sparser

"Therefore we have concluded that either the eIF-4AI and the eIF-4AII proteins were resolved from each other in the purification of rabbit reticulocyte eIF-4A or that eIF-4AII preferentially associates with the p220 and p24 subunits of eIF-4F. Evidence favoring the latter possibility is discussed."

2241157

PRPF8 binds EIF4G1 and docking protein-1. 1 / 1

| 1

➶

sparser

"As a polymeric protein, pp62 protein is co-assembled with another polymeric protein, pp220, to form the inner core shell of ASFV ( xref )."

35097046

PRPF8 affects CD2BP2

6 |

CD2BP2 binds PRPF8. 6 / 6

6 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

27173435

➶

biogrid

No evidence text available

17353931

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

22939629

PRPF19 affects PRPF8

5 | 1

PRPF19 binds PRPF8.

5 |

PRPF8 binds PRPF19. 5 / 5

5 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

22939629

PRPF19 ubiquitinates PRPF8.

| 1

PRPF19 ubiquitinates PRPF8. 1 / 1

| 1

➶

sparser

"On the other hand, ubiquitination of PRP8 at the C-terminal by PRP19 was not as significant and PRP4B kinase and USP39 were not ubiquitinated by PRP19."

28088760

PRP8 affects PRPF8

| 6

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

PRPF8 binds CDC40 and PRP8. 1 / 1

| 1

➶

sparser

"On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction."

10628969

| 6

PRPF8 binds TMED9, EIF4G1, HRES1, and LCN2. 2 / 2

| 2

➶

sparser

7880899

➶

sparser

"Chemical cross-linking of eIF-4F to cap-labeled mRNA, showed that phosphorylation increased the interaction of both the p25 and p220 subunits of eIF-4F with the 5' end of mRNA."

2002015

TP73 binds PRPF8, EIF4G1, and capsid protein. 2 / 2

| 2

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold 1998, Cobbold 1996, Heath 2003)."

17765705

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold and Wileman, 1998; Cobbold et al., 1996; Heath et al., 2003) ."

17765705

TMED2 binds CD9, PRPF8, TPPP, and EIF4G1. 1 / 1

| 1

➶

sparser

2241157

PRPF8 binds EIF4G1 and docking protein-1. 1 / 1

| 1

➶

sparser

"As a polymeric protein, pp62 protein is co-assembled with another polymeric protein, pp220, to form the inner core shell of ASFV ( xref )."

35097046

CD2BP2 affects PRPF8

6 |

CD2BP2 binds PRPF8. 6 / 6

6 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

27173435

➶

biogrid

No evidence text available

17353931

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

22939629

SART1 affects PRPF8

3 | 1 1

SART1 binds PRPF8. 4 / 4

3 | 1

➶

biogrid

No evidence text available

11350945

➶

biogrid

No evidence text available

26344197

➶

reach

"An independent set of 88 NBs in which survival outcome was also known showed an association between high SART1 and PRPF8 with inferior event-free survival (EFS) and overall survival and with SF3B1 with EFS alone (XREF_SUPPLEMENTARY)."

24556687

➶

biogrid

No evidence text available

22939629

SART1 binds SF3B1, UBL5, and PRPF8. 1 / 1

| 1

➶

sparser

"Using an expression data set of 649 NBs with associated prognostic information, the poor prognosis variables of age >18 months at diagnosis, stage 4 disease and MYCN amplification were all highly associated with high expression of SART1 , UBL5 , PRPF8 and SF3B1 at P <0.001, except for PRPF8 and MYCN amplification ( P =0.11; xref )."

24556687

PRPF8 affects PRPF6

5 |

PRPF6 binds PRPF8. 5 / 5

5 |

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

16723661

➶

biogrid

No evidence text available

27173435

➶

biogrid

No evidence text available

16723661

➶

biogrid

No evidence text available

22939629

PRPF8 affects PRPF19

5 |

PRPF8 binds PRPF19. 5 / 5

5 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

22939629

PRPF6 affects PRPF8

5 |

PRPF6 binds PRPF8. 5 / 5

5 |

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

16723661

➶

biogrid

No evidence text available

27173435

➶

biogrid

No evidence text available

16723661

➶

biogrid

No evidence text available

22939629

WDR83 affects PRPF8

4 |

PRPF8 binds WDR83. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

TSSC4 affects PRPF8

3 | 1

TSSC4 binds PRPF8. 3 / 3

3 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

26186194

PRPF8 binds SNRNP200 and TSSC4. 1 / 1

| 1

➶

sparser

34131137

SNW1 affects PRPF8

3 | 1

SNW1 binds PRPF8. 3 / 3

3 |

➶

biogrid

No evidence text available

12840015

➶

biogrid

No evidence text available

20467437

➶

biogrid

No evidence text available

26344197

SNW1 binds PRPF8 and CWC15. 1 / 1

| 1

➶

sparser

"Finally, PPIG interacts with SNW1, CWC15, and PRPF8, all through the isomerase domain ( xref )."

30518120

SLU7 affects PRPF8

4 |

SLU7 binds PRPF8. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

SF3B2 affects PRPF8

4 |

SF3B2 binds PRPF8. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

SF3B1 affects PRPF8

1 | 3

SART1 binds SF3B1, UBL5, and PRPF8. 1 / 1

| 1

➶

sparser

24556687

SF3B1 binds PRPF8. 1 / 1

1 |

➶

biogrid

No evidence text available

22939629

SF3B1 binds U2AF1, PRPF8, and PRPF3. 1 / 1

| 1

➶

sparser

28703423

SF3B1 binds PRPF8 and ribE. 1 / 1

| 1

➶

sparser

"Nevertheless, the association of SF3B1 and PRPF8 mutations with the RS phenotype, suggests that there may be common genes or pathways affected by both SF3B1 and PRPF8 ."

24781015

RNF113A affects PRPF8

| 4

RNF113A activates PRPF8.

| 3

RNF113A activates PRPF8. 3 / 3

| 3

➶

reach

"Cwc24 modulates the interaction of Prp8 with the 5' splice site."

31504764

➶

reach

"Nevertheless, Prp8 crosslinking was more specific in the presence of Cwc24 (lane 8), and spread through positions -3 to -9 in the absence of Cwc24 (lane 7) (tracing of lanes 7 and 8 shown in Supplementary Figure S2), suggesting that Cwc24 may promote specific interactions of Prp8 with the 5′ splice site.Interestingly, our previous study demonstrated that Prp8 and Cwc24 displayed major crosslinks at positions +1 and +2 of the 5′ splice site on post-activation spliceosomes (32)."

31504764

➶

reach

"The absence of NTC or Cwc24 allows Prp8 to interact immediately with the 5′ splice site upon the release of U4, but results in mislocalization of U5 and U6 on the 5′ splice site.A model for how Cwc24 may mediate formation of the RNA catalytic core through Prp2-mediated spliceosome remodeling is presented in Figure 8. Upon the release of U4, Cwc24 may bind onto the 5′ splice site immediately upon the binding of NTC, with its ZF-domain contacting the +1 and +2 positions of the intron."

31504764

RNF113A inhibits PRPF8.

| 1

RNF113A inhibits PRPF8. 1 / 1

| 1

➶

reach

"Interestingly, Prp8 was not detected to crosslink to the 5′ splice site on the Cwc24-associated spliceosomes, except for rather weak crosslinks at the -5 and -6 positions, indicating that the presence of Cwc24 prevents Prp8 from contacting the 5′ splice site on activated spliceosomes."

31504764

PRPF8 affects WDR83

4 |

PRPF8 binds WDR83. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF8 affects TSSC4

3 | 1

TSSC4 binds PRPF8. 3 / 3

3 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

26186194

PRPF8 binds SNRNP200 and TSSC4. 1 / 1

| 1

➶

sparser

34131137

PRPF8 affects SNW1

3 | 1

SNW1 binds PRPF8. 3 / 3

3 |

➶

biogrid

No evidence text available

12840015

➶

biogrid

No evidence text available

20467437

➶

biogrid

No evidence text available

26344197

SNW1 binds PRPF8 and CWC15. 1 / 1

| 1

➶

sparser

"Finally, PPIG interacts with SNW1, CWC15, and PRPF8, all through the isomerase domain ( xref )."

30518120

PRPF8 affects SLU7

4 |

SLU7 binds PRPF8. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF8 affects SF3B2

4 |

SF3B2 binds PRPF8. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF8 affects SF3B1

1 | 3

SART1 binds SF3B1, UBL5, and PRPF8. 1 / 1

| 1

➶

sparser

24556687

SF3B1 binds PRPF8. 1 / 1

1 |

➶

biogrid

No evidence text available

22939629

SF3B1 binds U2AF1, PRPF8, and PRPF3. 1 / 1

| 1

➶

sparser

28703423

SF3B1 binds PRPF8 and ribE. 1 / 1

| 1

➶

sparser

"Nevertheless, the association of SF3B1 and PRPF8 mutations with the RS phenotype, suggests that there may be common genes or pathways affected by both SF3B1 and PRPF8 ."

24781015

PRPF8 affects PRPF31

2 | 1 1

PRPF8 binds PRPF31.

2 | 1

PRPF31 binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

28088760

PRPF31 binds PRPF8, PRPF3, SNRNP200, and ATXN3. 1 / 1

| 1

➶

sparser

22819011

PRPF8 activates PRPF31.

| 1

PRPF8 activates PRPF31. 1 / 1

| 1

➶

reach

"Mutations of the splicing factor genes PRPF31, PRPF8, and HPRP3 cause RP11, 13, and 18, respectively (Baehr and Chen, 2001; Chakarova et al., 2002)."

14659595

PRPF8 affects PLIN2

| 4

PRPF8 activates PLIN2. 4 / 4

| 4

➶

reach

"PRPF8 C-terminal mutations cause an early onset and severe form of adRP, and structural analysis of this region shows that it inserts into the RNA binding tunnel of the SNRNP200 helicase."

26593421

➶

reach

"In addition, both truncation and missense mutations in PRPF8 have been reported to cause adRP, but the pathogenicity of missense mutations in PRPF8 is suspicious in some cases since missense variants in PRPF8 are common based on the gnomAD database; rare damaging variants in PRPF8 had a sum allele frequency of 2.17 × 10 (614/282896), which is higher than the prevalence of RP (1/3000)."

30689892

➶

reach

"Nine mutations, six of which are novel, in the pre-mRNA splicing-factor genes PRPF3, PRPF8, and PRPF31, causing adRP have been identified in the Spanish population."

12714658

➶

reach

"Relevant to both these interpretations is the observation that adRP linked to chromosome 17p is caused by mutations in PRPC8 (McKie et al., 2001), the human ortholog of yeast splicing factor Prp8p (Br[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

11545739

PRPF8 affects PCBP1

4 |

PRPF8 binds PCBP1. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF8 affects MT-ATP6

| 1 1 2

PRPF8 binds MT-ATP6.

| 2

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

PRPF8 activates MT-ATP6.

| 1 1

PRPF8 activates MT-ATP6. 2 / 2

| 1 1

➶

reach

"The spliceosome is composed of 5 small nuclear RNAs (snRNAs), U1–U5, and many proteins including pre-mRNA splicing factors PRPF3, PRPF4, PRPF6, PRPF8, and SNRNP200, all of which are also genetic causes of RP (Ruzickova and Stanek, 2016)."

30967900

➶

eidos

"Interestingly , while Snu114p / EFTUD2 interacts with both Brr2p / SNRNP200 and Prp8p / PRPF8 , EFTUD2 is associated with a craniofacial phenotype while SNRNP200 and PRPF8 both cause RP ( Figure 1 ) ."

33584830

PRPF8 affects Cell Survival

| 4

PRPF8 activates Cell Survival. 4 / 4

| 4

➶

eidos

"Functionally , the loss of Prp8 may specifically inhibit cell viability and metastasis and activated EMT and PI3K / Akt in HCC cells ."

32547101

➶

eidos

"Upregulation of Prp8 promoted cell viability and migration in a human hepatic astrocyte line ."

32547101

➶

eidos

"The upregulation of Prp8 promoted cell viability , metastasis and the activity of the PI3K / Akt pathway in hepatic astrocytes cells and HCC cells ."

32547101

➶

eidos

"Upregulation of Prp8 promoted cell viability and migration in HCC cells ."

32547101

PRPF8 affects Bloc1s3

| 2 2

PRPF8 binds Bloc1s3.

| 2

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

PRPF8 activates Bloc1s3.

| 2

PRPF8 activates Bloc1s3. 2 / 2

| 2

➶

reach

"For example, a previous study suggested that mutations in PRPF8 may cause RP by disrupting the nuclear association of Brr2p with the U5 snRNP and, consequently, the assembly of the triple snRNP."

19878916

➶

reach

"Herein, we sought to correlate the retinal cell-type-specific disease phenotype with the splicing profile shown by a patient with autosomal recessive RP, caused by a mutation in pre-mRNA splicing factor 8 (PRPF8)."

33994920

PRPF8 affects BCAS2

4 |

PRPF8 binds BCAS2. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF31 affects PRPF8

2 | 1 1

PRPF31 binds PRPF8.

2 | 1

PRPF31 binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

28088760

PRPF31 binds PRPF8, PRPF3, SNRNP200, and ATXN3. 1 / 1

| 1

➶

sparser

22819011

PRPF31 activates PRPF8.

| 1

PRPF31 activates PRPF8. 1 / 1

| 1

➶

reach

"Ubiquitination of Prp31 increases its affinity for Prp8 and this PTM is reversed by USP15."

| PMC

PCBP1 affects PRPF8

4 |

PRPF8 binds PCBP1. 4 / 4

4 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

HSP90 affects PRPF8

| 3 1

HSP90 binds PRPF8.

| 2 1

HSP90 binds PRPF8. 2 / 2

| 2

➶

reach

"The HSP90 and R2TP machinery binds unassembled cytoplasmic forms of PRPF8 and EFTUD2."

28515276

➶

reach

"In this study, we show that the U5 specific proteins PRPF8 and EFTUD2 interact in vivo with HSP90 and R2TP and that these interactions arise early in the cytoplasm."

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

17alpha-ethynylestradiol affects PRPF8

4 |

17alpha-ethynylestradiol increases the amount of PRPF8.

3 |

17alpha-ethynylestradiol increases the amount of PRPF8. 3 / 3

3 |

➶

ctd

No evidence text available

17108234

➶

ctd

No evidence text available

17942748

➶

ctd

No evidence text available

24096037

17alpha-ethynylestradiol decreases the amount of PRPF8.

sparser

"This is in agreement with the association of PRPF8 and RBM22 with intron lariats as part of the human catalytic step I spliceosome xref ."

31570875

PRPF8 binds RBM22 and TPS-sab. 1 / 1

| 1

➶

2 | 1

PRPF8 binds.

2 |

PRPF8 binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

17317632

➶

biogrid

No evidence text available

16723661

PRPF8 translocates.

| 1

PRPF8 translocates to the nucleus. 1 / 1

| 1

➶

reach

"PRPF8 is synthesized in the cytoplasm and must be transported to the nucleus to perform its role in splicing."

28515276

PRPF8 affects apoptotic process

| 3

PRPF8 inhibits apoptotic process. 3 / 3

| 3

➶

reach

"Consequently, circRNA-UBAP2 upregulated expression level of PRPF8 by sponged miR-382-5p acted as a ceRNA, promoted proliferation and inhibited apoptosis in ovarian cancer cells."

32690086

➶

reach

"Knockdown of pan-cancer drivers PRPF8 caused cell apoptosis in breast cancer, which dedicated that PRPF8 is a proto-oncogene regulating cell viability [10]."

32690086

➶

reach

34504842

PRPF8 affects ZNF830

3 |

PRPF8 binds ZNF830. 3 / 3

3 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF8 affects SNRPE

3 |

SNRPE binds PRPF8. 3 / 3

3 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

22939629

➶

biogrid

No evidence text available

26344197

PRPF8 affects SF3B4

3 |

SF3B4 binds PRPF8. 3 / 3

3 |

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

➶

biogrid

No evidence text available

22365833

PRPF8 affects RBM22

| 1 2

PRPF8 binds RBM22. 2 / 2

| 1 1

➶

reach

35474897

➶

sparser

"This is in agreement with the association of PRPF8 and RBM22 with intron lariats as part of the human catalytic step I spliceosome xref ."

31570875

PRPF8 binds RBM22 and TPS-sab. 1 / 1

| 1

➶

3 |

PRPF8 binds PRPF4. 3 / 3

3 |

➶

biogrid

No evidence text available

19615732

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

26496610

PRPF8 affects ISPH

| 3

PRPF8 inhibits ISPH.

| 2

PRPF8 inhibits ISPH. 2 / 2

| 2

➶

reach

"Depletion of PRPF8 and treatment with a small molecule spliceosome inhibitor (PlaB) disrupts HDR, SSA, and end resection."

29212152

➶

reach

"Using two independent siRNAs targeting PRPF8 (siPRPF8-2, siPRPF8-4), as well as a previously described siRNA targeting XAB2 (siXAB2-4), we found that PRPF8 and XAB2 depletion cause a significantly greater decrease in the frequency of HDR and SSA, compared to EJ."

29212152

PRPF8 activates ISPH.

| 1

PRPF8 activates ISPH. 1 / 1

| 1

➶

reach

"We also found that transient expression of a myc tagged siRNA resistant PRPF8 rescued the HDR and SSA defects caused by siPRPF8-2 treatment."

29212152

PRPF8 affects HSP90

| 2 1

HSP90 binds PRPF8. 2 / 2

| 2

➶

reach

"The HSP90 and R2TP machinery binds unassembled cytoplasmic forms of PRPF8 and EFTUD2."

28515276

➶

reach

"In this study, we show that the U5 specific proteins PRPF8 and EFTUD2 interact in vivo with HSP90 and R2TP and that these interactions arise early in the cytoplasm."

1 | 2

PRPF8 inhibits E6.

sparser

"Collectively, these data provide strong support for the idea that PRPF8 associates with EFTUD2 and AAR2 early in its assembly pathway."

| 1 2

Bloc1s3 binds PRPF8.

| 2

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

Bloc1s3 activates PRPF8.

| 1

Bloc1s3 activates PRPF8. 1 / 1

| 2

➶

sparser

"P220 is phosphorylated by p34cdc2 kinase and MAP kinase in M phase, and concomitantly loses its MT-binding and MT-stabilizing activities."

15216896

➶

| 2

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold and Wileman, 1998; Cobbold et al., 1996; Heath et al., 2003) ."

17765705

ZNHIT2 affects PRPF8

2 |

ZNHIT2 binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

28561026

U2AF2 affects PRPF8

1 | 1

PRPF8 binds U2AF2. 2 / 2

1 | 1

➶

biogrid

No evidence text available

26641092

➶

reach

35474897

U2AF1 affects PRPF8

1 | 1

U2AF1 binds PRPF8. 1 / 1

1 |

➶

biogrid

No evidence text available

22939629

SF3B1 binds U2AF1, PRPF8, and PRPF3. 1 / 1

| 1

➶

sparser

28703423

TP73 affects capsid protein

| 2

TP73 binds PRPF8, EIF4G1, and capsid protein. 2 / 2

| 2

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold 1998, Cobbold 1996, Heath 2003)."

17765705

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold and Wileman, 1998; Cobbold et al., 1996; Heath et al., 2003) ."

17765705

TMED9 affects PRPF8

| 2

PRPF8 binds TMED9, EIF4G1, HRES1, and LCN2. 2 / 2

| 2

➶

sparser

7880899

➶

sparser

"Chemical cross-linking of eIF-4F to cap-labeled mRNA, showed that phosphorylation increased the interaction of both the p25 and p220 subunits of eIF-4F with the 5' end of mRNA."

2002015

SRRM2 affects PRPF8

1 | 1

SRRM2 binds PRPF8 and PRP8. 1 / 1

| 1

1 |

➶

biogrid

No evidence text available

28561026

RUVBL2 binds PRPF8 and EFTUD2. 1 / 1

| 1

➶

sparser

"Association of RUVBL2 with either EFTUD2 or PRPF8 was greatly compromised attesting to the efficiency of the knockdown ( xref , xref )."

28561026

RPAP3 affects PRPF8

| 1 1

PRPF8 binds RPAP3. 2 / 2

| 1 1

➶

reach

"This demonstrated that the interaction between PRPF8 and RPAP3 can take place in the cytoplasm and is independent of EFTUD2."

28515276

➶

2 |

RNPS1 binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

26344197

➶

biogrid

No evidence text available

17353931

R2TP affects EFTUD2

| 2

HSP90 binds PRPF8, EFTUD2, and R2TP. 1 / 1

| 1

➶

sparser

"In this study, we show that the U5-specific proteins PRPF8 and EFTUD2 interact in vivo with HSP90 and R2TP and that these interactions arise early in the cytoplasm."

28515276

PRPF8 binds EFTUD2 and R2TP. 1 / 1

| 1

➶

sparser

"Depletion of U5 proteins and PRPF8 RP mutation both increase the association of PRPF8 and EFTUD2 with R2TP."

28515276

PRPF8 affects ribE

| 2

PRPF8 binds ribE. 1 / 1

| 1

➶

1 | 1

➶

biogrid

No evidence text available

26641092

➶

reach

35474897

PRPF8 affects U2AF1

1 | 1

U2AF1 binds PRPF8. 1 / 1

1 |

| 2

➶

sparser

7880899

➶

sparser

"Chemical cross-linking of eIF-4F to cap-labeled mRNA, showed that phosphorylation increased the interaction of both the p25 and p220 subunits of eIF-4F with the 5' end of mRNA."

2002015

PRPF8 affects SRRM2

1 | 1

SRRM2 binds PRPF8 and PRP8. 1 / 1

| 1

1 |

➶

biogrid

No evidence text available

28561026

RUVBL2 binds PRPF8 and EFTUD2. 1 / 1

| 1

➶

sparser

"Association of RUVBL2 with either EFTUD2 or PRPF8 was greatly compromised attesting to the efficiency of the knockdown ( xref , xref )."

28561026

PRPF8 affects RPAP3

| 1 1

PRPF8 binds RPAP3. 2 / 2

| 1 1

➶

reach

"This demonstrated that the interaction between PRPF8 and RPAP3 can take place in the cytoplasm and is independent of EFTUD2."

28515276

➶

| 2

PRPF8 inhibits RO60.

| 1

PRPF8 inhibits RO60. 1 / 1

| 1

➶

reach

29212152

PRPF8 activates RO60.

| 2

PRPF8 activates Neoplasm Metastasis. 2 / 2

| 2

➶

eidos

"Functionally , the loss of Prp8 may specifically inhibit cell viability and metastasis and activated EMT and PI3K / Akt in HCC cells ."

32547101

➶

eidos

"The upregulation of Prp8 promoted cell viability , metastasis and the activity of the PI3K / Akt pathway in hepatic astrocytes cells and HCC cells ."

32547101

PRPF8 affects M

2 |

PRPF8 binds M. 1 / 1

1 |

➶

virhostnet

No evidence text available

25464832

PRPF8 binds M. 1 / 1

1 |

➶

virhostnet

No evidence text available

25464832

PRPF8 affects LCN2

| 2

2 |

PRPF8 binds EAPP. 2 / 2

2 |

➶

biogrid

No evidence text available

28561026

➶

biogrid

No evidence text available

28514442

PRPF8 affects Death

| 2

PRPF8 activates Death. 1 / 1

| 1

| 1

➶

sparser

33584830

PRPF8 binds COPS5. 1 / 1

| 1

➶

sparser

"For crystallographic analyses, we used N-terminally truncated hBrr2 T1 in complex with the Jab1 domain of the hPrp8 protein lacking a Brr2-inhibiting C-terminal tail (hJab1 ΔC )."

34048711

PRPF8 affects CDC40

1 | 1

PRPF8 binds CDC40 and PRP8. 1 / 1

| 1

➶

sparser

"On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction."

10628969

PRPF8 binds CDC40. 1 / 1

1 |

➶

biogrid

No evidence text available

26344197

PRPF8 affects BSG

2 |

BSG binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

PRPF8 affects ATXN3

| 2

PRPF8 binds ATXN3. 1 / 1

| 1

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

PA affects PRPF8

2 |

PRPF8 binds PA. 1 / 1

1 |

➶

virhostnet

No evidence text available

26789921

PRPF8 binds PA. 1 / 1

1 |

➶

virhostnet

No evidence text available

25464832

MT-ATP6 affects PRPF8

| 2

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

M affects PRPF8

2 |

PRPF8 binds M. 1 / 1

1 |

➶

virhostnet

No evidence text available

25464832

PRPF8 binds M. 1 / 1

1 |

➶

virhostnet

No evidence text available

25464832

LCN2 affects TMED9

| 2

| 2

➶

sparser

7880899

➶

sparser

"Chemical cross-linking of eIF-4F to cap-labeled mRNA, showed that phosphorylation increased the interaction of both the p25 and p220 subunits of eIF-4F with the 5' end of mRNA."

2002015

EIF4G1 affects PRPF8, TP73, and capsid protein

| 2

TP73 binds PRPF8, EIF4G1, and capsid protein. 2 / 2

| 2

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold 1998, Cobbold 1996, Heath 2003)."

17765705

➶

sparser

"The p73 capsid protein and pp220 polyprotein associate with membranes before assembly into viruses (Cobbold and Wileman, 1998; Cobbold et al., 1996; Heath et al., 2003) ."

17765705

EIF4G1 affects LCN2

| 2

PRPF8 binds TMED9, EIF4G1, HRES1, and LCN2. 2 / 2

| 2

➶

sparser

7880899

➶

2 |

PRPF8 binds EBNA-LP. 1 / 1

1 |

➶

| 1

➶

sparser

33584830

PRPF8 binds COPS5. 1 / 1

| 1

➶

sparser

"For crystallographic analyses, we used N-terminally truncated hBrr2 T1 in complex with the Jab1 domain of the hPrp8 protein lacking a Brr2-inhibiting C-terminal tail (hJab1 ΔC )."

34048711

CDC40 affects PRPF8

1 | 1

PRPF8 binds CDC40 and PRP8. 1 / 1

| 1

➶

sparser

"On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction."

10628969

PRPF8 binds CDC40. 1 / 1

1 |

➶

biogrid

No evidence text available

26344197

Bloc1s3 affects PRP8

| 2

PRPF8 binds MT-ATP6, PRP8, and Bloc1s3. 2 / 2

| 2

➶

sparser

17934474

➶

sparser

"Compared to wild-type Prp8p ( xref ), the Prp8-rp mutant proteins were associated with less Brr2p ( xref , lanes 1-7) and with more Aar2p ( xref , lanes 9-13)."

17934474

BSG affects PRPF8

2 |

BSG binds PRPF8. 2 / 2

2 |

➶

biogrid

No evidence text available

28514442

➶

biogrid

No evidence text available

26186194

ATXN3 affects PRPF8

| 2

PRPF8 binds ATXN3. 1 / 1

| 1

➶

sparser

34131137

SNRNP200 affects SNRNP40

| 1

PRPF8 binds SNRNP40, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"Because Snrnp40 is tightly bound to Prpf8, Eftud2, and Snrnp200, it is likely that Snrnp40 also participates and facilitates these processes."

31427773

SNRNP200 affects PRPF31

| 1

PRPF31 binds PRPF8, PRPF3, SNRNP200, and ATXN3. 1 / 1

| 1

➶

sparser

22819011

SNRNP200 affects LAP3

| 1

PRPF8 binds LAP3, EFTUD2, and SNRNP200. 1 / 1

| 1

➶

sparser

"The proteomic analysis of the GFP IPs showed a strong association of PRPF8-LAP with its partners EFTUD2 and SNRNP200, and conversely, GFP-EFTUD2 strongly bound PRPF8 and SNRNP200."

28515276