IndraLab

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"For localization studies of PRPF8 and XAB2, cells were treated with 10 Gy of IR, but allowed to recover for 30 min, and were treated with pre-extraction buffer (20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 300 mM sucrose, 0.25% Triton-X 100) just prior to fixation."