IndraLab
Statements
reach
"The absence of NTC or Cwc24 allows Prp8 to interact immediately with the 5′ splice site upon the release of U4, but results in mislocalization of U5 and U6 on the 5′ splice site.A model for how Cwc24 may mediate formation of the RNA catalytic core through Prp2-mediated spliceosome remodeling is presented in Figure 8. Upon the release of U4, Cwc24 may bind onto the 5′ splice site immediately upon the binding of NTC, with its ZF-domain contacting the +1 and +2 positions of the intron."