IndraLab
Statements
reach
"Nevertheless, Prp8 crosslinking was more specific in the presence of Cwc24 (lane 8), and spread through positions -3 to -9 in the absence of Cwc24 (lane 7) (tracing of lanes 7 and 8 shown in Supplementary Figure S2), suggesting that Cwc24 may promote specific interactions of Prp8 with the 5′ splice site.Interestingly, our previous study demonstrated that Prp8 and Cwc24 displayed major crosslinks at positions +1 and +2 of the 5′ splice site on post-activation spliceosomes (32)."
reach
"The absence of NTC or Cwc24 allows Prp8 to interact immediately with the 5′ splice site upon the release of U4, but results in mislocalization of U5 and U6 on the 5′ splice site.A model for how Cwc24 may mediate formation of the RNA catalytic core through Prp2-mediated spliceosome remodeling is presented in Figure 8. Upon the release of U4, Cwc24 may bind onto the 5′ splice site immediately upon the binding of NTC, with its ZF-domain contacting the +1 and +2 positions of the intron."