IndraLab
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"To interrogate the functionality of the modeled interaction between SNU66, SNRP27, and PRP8, we used a CRISPR–cas9 system coupled with an oligonucleotide-directed recombination repair-based screen to randomize snu-66 at position H765 and prp-8 at position H1573, and screened for viable mutants.We initially recovered nine independent strains, with five different amino acid substitutions at snu-66 position H765; G, L, S, C, and T."